Rw0G022460
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01044
OG0004085
Physical Location & Seq
Reverse (-)
12364 .. 14196
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022460.1

Sequence Viewer

Length: 1443 bp
ATGGCAGCCGGGTTAGTGATAACAAGTGAATACAGGCATTACAATGGCAAGTTGACCCTGATTGTGGTAATATCTTGCATGGTTGCTGCCATGGGAGGTATCATTTTCGGCTATGATATTGGAATTTCAGGTGGAGTGACTTCAATGGAACCATTTCTGAAGAAATTTTTCCCAGAGGTTTACAGAAAGATGAAAGAAGACACGAAAGTCAGCAACTACTGCAAATTTGATAGCCATCTTTTAACCACGTTTACATCCTCTCTCTACATAGCTGGCCTTGTAGCTTCCTTCTTTGCCTCTTCTATAACCGGAGCATTTGGCCGCAAGCCTTCGATCCTGGTAGCTGGTGCCTCATTCCTTGCTGGTTCGGCTCTAGGTGGCGCAGCTGTTAACTTGTTCATGCTAATATCTGGTCGCATCTTGCTTGGTATTGGAGTTGGCTTTGGAAACCAGGCAGTCCCACTTTATCTCTCAGAAATGGCACCCACCAACATTCAGAGGAGCAATCAACATAGGCTTCCAATTATGTGTTTGGCATTGGAGTACTATCAGCTAACCTCATCAACTTTGGCACCGAAAAGATCAGAAGCGGCTGGGGTTGGCGAATCTCCCCTAGCCATGGCAGCAGTTCCAGCTGCATTACTAACACTAGGTGCTCTTTTCCTACCAGAAACACCAAACAGCTTAATCCAACGCACAAATGACCACGAAAGGGCCAAGCTGATGCTACAACGTACACGAGGAATGGTGGATGTGCAAGCGGAACTGGATGATCTCATCGAAGCAAGTAACAAATCAAAAACAATCGATCACCCTTTCAGAAAAATTGTACAAAGAAAGTATAGGCCTCAACTTCTGACTGGAATAAACGTCATCTCTTTCTATGCTCCAATCCTCTTTCGGACACTCGGTTTAAGTGAAAGCATGTCACTTTTGTCTGCAGTCATTAGCACAGGAGTGGTGGGGACTGCAGCGACCTTCCTATCAATGCTTATGATGTTTTTGTCCCAAATCATAGTAGGCAGCATTATGGCAGCTAAGCTTGGTGACGTTGGTGGCTTGAGCAAAGTATATGCATATTTGGTGCTGTTCTTCGTATGTATATATGTAGCTGGGTTTGCGTGGTCATGGGGGCCTTTAGGATGGTTGATTCCGAGTGAAATTTTTCCGTTGGAGATTCGATCAGCTGGACAAAGTATTACAGTGGCAGTGAACTTTCTATTCACTTTTGGTATTGCTCAGACATTTCTTTCTATGCTTTGCTGCTTCAAGTCTGGTATCTTCTTTTTCTTTGGAGGATGGGTGATGGTAATGACTGCATTTGTGTACTTCTTGTTGCCTGAGACTACAAATATTCCTATTGAGAAAATGGATGAAGTGTGGGGAGAGCATTGGTTTTGGAAGAGGATTGTGGGTGAAGTGAGGGAGGACATTAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

480

Amino Acids

52.53

Weight (kDa)

8.87

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 26 - 461 8.8e-75 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccB1I GGYRCC 3 cut(s) 347, 481, 571
AciI CCGC 3 cut(s) 322, 590, 761
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 4 cut(s) 123, 164, 224, 1161
AcuI CTGAAG 1 cut(s) 179
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 4 cut(s) 545, 736, 831, 1328
AfiI CCNNNNNNNGG 3 cut(s) 64, 619, 712
AgsI TTSAA 2 cut(s) 144, 1270
AjnI CCWGG 2 cut(s) 336, 450
AleI CACNNNNGTG 1 cut(s) 956
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 1 cut(s) 1337
AlwI GGATC 1 cut(s) 328
AoxI GGCC 5 cut(s) 274, 319, 714, 845, 1133
ApeKI GCWGC 9 cut(s) 5, 86, 383, 623, 635, 971, 1023, 1034, 1263
ApoI RAATTY 4 cut(s) 123, 164, 224, 1161
Asp700I GAANNNNTTC 3 cut(s) 153, 167, 1164
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 714, 1133
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 4 cut(s) 803, 1058, 1315, 1427
BanI GGYRCC 3 cut(s) 347, 481, 571
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 9 cut(s) 17, 73, 395, 622, 635, 983, 1035, 1046, 1250
BccI CCATC 4 cut(s) 243, 1137, 1293, 1300
BciT130I CCWGG 2 cut(s) 338, 452
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 1337
BfaI CTAG 3 cut(s) 374, 614, 650
BfmI CTRYAG 2 cut(s) 939, 969
BlpI GCTNAGC 1 cut(s) 1038
BmcAI AGTACT 1 cut(s) 545
Bme1390I CCNGG 3 cut(s) 10, 338, 452
BmgT120I GGNCC 2 cut(s) 714, 1133
BmiI GGNNCC 5 cut(s) 150, 349, 483, 573, 1134
BmrFI CCNGG 3 cut(s) 10, 338, 452
BmsI GCATC 2 cut(s) 426, 714
BpiI GAAGAC 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 1038
BpuEI CTTGAG 1 cut(s) 1081
BpuMI CCSGG 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 807
BsaBI GATNNNNATC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 90, 618
BsaWI WCCGGW 1 cut(s) 308
BsaXI ACNNNNNCTCC 2 cut(s) 1287, 1317
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 619, 712
Bse1I ACTGG 2 cut(s) 771, 865
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 2 cut(s) 338, 452
BseCI ATCGAT 1 cut(s) 807
BseDI CCNNGG 2 cut(s) 90, 618
BseGI GGATG 6 cut(s) 254, 757, 775, 1148, 1304, 1378
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 3 cut(s) 64, 619, 712
BseMII CTCAG 3 cut(s) 486, 1253, 1332
BseNI ACTGG 2 cut(s) 771, 865
BseRI GAGGAG 1 cut(s) 514
BseXI GCAGC 9 cut(s) 17, 73, 395, 622, 635, 983, 1035, 1046, 1250
BseYI CCCAGC 2 cut(s) 593, 1112
BshFI GGCC 5 cut(s) 276, 321, 716, 847, 1135
BshNI GGYRCC 3 cut(s) 347, 481, 571
BshVI ATCGAT 1 cut(s) 807
BsiHKAI GWGCWC 1 cut(s) 658
BsiSI CCGG 2 cut(s) 9, 309
BslFI GGGAC 3 cut(s) 443, 979, 991
BslI CCNNNNNNNGG 3 cut(s) 64, 619, 712
BsmAI GTCTC 1 cut(s) 1337
BsmFI GGGAC 3 cut(s) 443, 979, 991
BsnI GGCC 5 cut(s) 276, 321, 716, 847, 1135
Bsp1286I GDGCHC 1 cut(s) 658
Bsp1407I TGTACA 1 cut(s) 829
Bsp143I GATC 5 cut(s) 333, 581, 772, 808, 1181
Bsp1720I GCTNAGC 1 cut(s) 1038
Bsp19I CCATGG 2 cut(s) 90, 618
BspACI CCGC 3 cut(s) 322, 590, 761
BspANI GGCC 5 cut(s) 276, 321, 716, 847, 1135
BspCNI CTCAG 3 cut(s) 485, 1252, 1333
BspDI ATCGAT 1 cut(s) 807
BspLI GGNNCC 5 cut(s) 150, 349, 483, 573, 1134
BspMAI CTGCAG 2 cut(s) 943, 973
BspPI GGATC 1 cut(s) 328
BspT107I GGYRCC 3 cut(s) 347, 481, 571
BsrGI TGTACA 1 cut(s) 829
BsrI ACTGG 2 cut(s) 771, 865
BssECI CCNNGG 2 cut(s) 90, 618
BssMI GATC 5 cut(s) 333, 581, 772, 808, 1181
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 2 cut(s) 90, 618
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 2 cut(s) 338, 452
Bst4CI ACNGT 1 cut(s) 1204
Bst6I CTCTTC 2 cut(s) 304, 1397
BstAPI GCANNNNNTGC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 829
BstC8I GCNNGC 3 cut(s) 274, 326, 759
BstDEI CTNAG 4 cut(s) 472, 1038, 1239, 1341
BstDSI CCRYGG 2 cut(s) 90, 618
BstF5I GGATG 6 cut(s) 254, 757, 775, 1148, 1304, 1378
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 5 cut(s) 336, 584, 775, 811, 1184
BstMAI GTCTC 1 cut(s) 1337
BstMBI GATC 5 cut(s) 333, 581, 772, 808, 1181
BstMWI GCNNNNNNNGC 5 cut(s) 219, 368, 623, 632, 1118
BstNI CCWGG 2 cut(s) 338, 452
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 3 cut(s) 8, 336, 450
BstSFI CTRYAG 2 cut(s) 939, 969
BstV1I GCAGC 9 cut(s) 17, 73, 395, 622, 635, 983, 1035, 1046, 1250
BstV2I GAAGAC 1 cut(s) 204
Bsu15I ATCGAT 1 cut(s) 807
BsuRI GGCC 5 cut(s) 276, 321, 716, 847, 1135
BsuTUI ATCGAT 1 cut(s) 807
BtgI CCRYGG 2 cut(s) 90, 618
BtsCI GGATG 6 cut(s) 254, 757, 775, 1148, 1304, 1378
BtsI GCAGTG 1 cut(s) 1215
BtsIMutI CAGTG 2 cut(s) 1209, 1215
Cac8I GCNNGC 3 cut(s) 274, 326, 759
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 714, 1133
ClaI ATCGAT 1 cut(s) 807
Csp6I GTAC 4 cut(s) 544, 735, 830, 1327
CviAII CATG 6 cut(s) 79, 91, 400, 619, 925, 1128
CviQI GTAC 4 cut(s) 544, 735, 830, 1327
DdeI CTNAG 4 cut(s) 472, 1038, 1239, 1341
DpnI GATC 5 cut(s) 335, 583, 774, 810, 1183
DpnII GATC 5 cut(s) 333, 581, 772, 808, 1181
DraIII CACNNNGTG 1 cut(s) 653
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 304, 1397
EarI CTCTTC 2 cut(s) 304, 1397
Eco130I CCWWGG 2 cut(s) 90, 618
Eco147I AGGCCT 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 1133
EcoRII CCWGG 2 cut(s) 336, 450
EcoT14I CCWWGG 2 cut(s) 90, 618
EcoT22I ATGCAT 1 cut(s) 1078
ErhI CCWWGG 2 cut(s) 90, 618
FaeI CATG 6 cut(s) 82, 94, 403, 622, 928, 1131
FaqI GGGAC 3 cut(s) 443, 979, 991
FatI CATG 6 cut(s) 78, 90, 399, 618, 924, 1127
FokI GGATG 6 cut(s) 241, 764, 782, 1155, 1311, 1385
FspBI CTAG 3 cut(s) 374, 614, 650
GlaI GCGC 1 cut(s) 382
GsaI CCCAGC 2 cut(s) 597, 1116
HaeIII GGCC 5 cut(s) 276, 321, 716, 847, 1135
HapII CCGG 2 cut(s) 9, 309
HhaI GCGC 1 cut(s) 383
Hin1II CATG 6 cut(s) 82, 94, 403, 622, 928, 1131
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HincII GTYRAC 2 cut(s) 54, 391
HindII GTYRAC 2 cut(s) 54, 391
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 3 cut(s) 605, 1150, 1177
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 9, 309
HphI GGTGA 4 cut(s) 803, 1058, 1315, 1427
Hpy166II GTNNAC 7 cut(s) 54, 181, 252, 391, 737, 1213, 1327
Hpy188I TCNGA 9 cut(s) 159, 475, 498, 586, 821, 858, 903, 1155, 1242
Hpy8I GTNNAC 7 cut(s) 54, 181, 252, 391, 737, 1213, 1327
HpyAV CCTTC 3 cut(s) 298, 339, 988
HpyCH4III ACNGT 1 cut(s) 1204
HpyCH4IV ACGT 4 cut(s) 248, 733, 870, 1050
HpyCH4V TGCA 8 cut(s) 78, 222, 638, 757, 941, 971, 1076, 1319
HpyF10VI GCNNNNNNNGC 5 cut(s) 219, 368, 623, 632, 1118
HpyF3I CTNAG 4 cut(s) 472, 1038, 1239, 1341
HpySE526I ACGT 4 cut(s) 248, 733, 870, 1050
Hsp92II CATG 6 cut(s) 82, 94, 403, 622, 928, 1131
HspAI GCGC 1 cut(s) 381
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 5 cut(s) 333, 581, 772, 808, 1181
LmnI GCTCC 3 cut(s) 311, 501, 892
Lsp1109I GCAGC 9 cut(s) 17, 73, 395, 622, 635, 983, 1035, 1046, 1250
LweI GCATC 2 cut(s) 426, 714
MaeI CTAG 3 cut(s) 374, 614, 650
MaeII ACGT 4 cut(s) 248, 733, 870, 1050
MaeIII GTNAC 4 cut(s) 136, 788, 927, 1046
MalI GATC 5 cut(s) 335, 583, 774, 810, 1183
MboI GATC 5 cut(s) 333, 581, 772, 808, 1181
MboII GAAGA 6 cut(s) 172, 209, 291, 1084, 1273, 1414
MhlI GDGCHC 1 cut(s) 658
MluCI AATT 6 cut(s) 123, 164, 224, 522, 825, 1161
MmeI TCCRAC 2 cut(s) 715, 1152
Mph1103I ATGCAT 1 cut(s) 1078
MroXI GAANNNNTTC 3 cut(s) 153, 167, 1164
MseI TTAA 5 cut(s) 242, 390, 686, 914, 1434
MslI CAYNNNNRTG 2 cut(s) 42, 956
MspA1I CMGCKG 3 cut(s) 386, 635, 1187
MspI CCGG 2 cut(s) 9, 309
MspR9I CCNGG 3 cut(s) 10, 338, 452
MvaI CCWGG 2 cut(s) 338, 452
MwoI GCNNNNNNNGC 5 cut(s) 219, 368, 623, 632, 1118
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 2 cut(s) 90, 618
NdeII GATC 5 cut(s) 333, 581, 772, 808, 1181
NlaIII CATG 6 cut(s) 82, 94, 403, 622, 928, 1131
NlaIV GGNNCC 5 cut(s) 150, 349, 483, 573, 1134
NmuCI GTSAC 3 cut(s) 136, 927, 1046
NsiI ATGCAT 1 cut(s) 1078
NspI RCATGY 1 cut(s) 928
OliI CACNNNNGTG 1 cut(s) 956
PceI AGGCCT 1 cut(s) 847
PdmI GAANNNNTTC 3 cut(s) 153, 167, 1164
PfeI GAWTC 3 cut(s) 605, 1150, 1177
Psp6I CCWGG 2 cut(s) 336, 450
PspFI CCCAGC 2 cut(s) 593, 1112
PspGI CCWGG 2 cut(s) 336, 450
PspN4I GGNNCC 5 cut(s) 150, 349, 483, 573, 1134
PspPI GGNCC 2 cut(s) 714, 1133
PstI CTGCAG 2 cut(s) 943, 973
PvuII CAGCTG 3 cut(s) 386, 635, 1187
RsaI GTAC 4 cut(s) 545, 736, 831, 1328
RsaNI GTAC 4 cut(s) 544, 735, 830, 1327
RseI CAYNNNNRTG 2 cut(s) 42, 956
SaqAI TTAA 5 cut(s) 242, 390, 686, 914, 1434
Sau3AI GATC 5 cut(s) 333, 581, 772, 808, 1181
Sau96I GGNCC 2 cut(s) 714, 1133
ScaI AGTACT 1 cut(s) 545
ScrFI CCNGG 3 cut(s) 10, 338, 452
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 2 cut(s) 426, 714
SfcI CTRYAG 2 cut(s) 939, 969
SmiMI CAYNNNNRTG 2 cut(s) 42, 956
SmlI CTYRAG 1 cut(s) 1060
SmoI CTYRAG 1 cut(s) 1060
Sse9I AATT 6 cut(s) 123, 164, 224, 522, 825, 1161
SseBI AGGCCT 1 cut(s) 847
SsiI CCGC 3 cut(s) 322, 590, 761
SspI AATATT 1 cut(s) 1354
SspMI CTAG 3 cut(s) 374, 614, 650
StuI AGGCCT 1 cut(s) 847
StyD4I CCNGG 3 cut(s) 8, 336, 450
StyI CCWWGG 2 cut(s) 90, 618
TaaI ACNGT 1 cut(s) 1204
TaiI ACGT 4 cut(s) 251, 736, 873, 1053
TaqI TCGA 4 cut(s) 332, 780, 807, 1180
TasI AATT 6 cut(s) 123, 164, 224, 522, 825, 1161
TatI WGTACW 3 cut(s) 543, 829, 1326
TauI GCSGC 2 cut(s) 324, 593
TfiI GAWTC 3 cut(s) 605, 1150, 1177
Tru1I TTAA 5 cut(s) 242, 390, 686, 914, 1434
Tru9I TTAA 5 cut(s) 242, 390, 686, 914, 1434
TscAI CASTG 2 cut(s) 1209, 1215
TseFI GTSAC 3 cut(s) 136, 927, 1046
TseI GCWGC 9 cut(s) 5, 86, 383, 623, 635, 971, 1023, 1034, 1263
Tsp45I GTSAC 3 cut(s) 136, 927, 1046
TspDTI ATGAA 3 cut(s) 206, 388, 1389
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 2 cut(s) 1209, 1215
XapI RAATTY 4 cut(s) 123, 164, 224, 1161
XceI RCATGY 1 cut(s) 928
XmnI GAANNNNTTC 3 cut(s) 153, 167, 1164
XspI CTAG 3 cut(s) 374, 614, 650
ZrmI AGTACT 1 cut(s) 545
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.