Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014434

Physical Location & Seq

Genomic Coordinates Reverse (-)

33540969 .. 33547441

Gene Structure 6473 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1267600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3369 bp

ATGGGAAAGCAAAAGCAGCAAGTTATTTCCCGCTTCTTCGCTCCCAAACCCAAAACCCCAGACCCCTCATCTCCTTCCTCCTCCTCATTTCCTCCCTCATCGCCGCCGTCAAACCCTAAAGACCCACCCACCCCACCTCCCAAAATCACAGCCACAGTCGCCTTCTCTCCCGCCAAACGCGCCCTCATCTCCTCCCACCTCGCCGTCTCTTCCTCTCCCAAACCAGCCAAACTCCCCAAACTCTCTCCTCACACCCACAACCCCATACCCGCCGCAGCCAATCCCTCCCTCCACCAAAAATTCCTCCAGAAGCTTCTAGAACCCGCCTCCGACGTTCCAGAACCTCCCCCTTCCTCCAACCAACCCGCCAAGTTCACGCCTTTGGAGCAGCAGGTGGTGGAATTGAAGAAGCGCCACCCGGATGTTCTTCTGATGGTGGAAGTCGGTTACAAGTACCGGTTTTTCGGCGAAGACGCCGAAATTGCTGCGAGGGTTTTGGGGATTTATGCCCACATGGATCACAATTTCTTGACCGCAAGTGTCCCAACCTTCCGGTTGAATGTCCACGTCAGGAGGCTGGTGGGTGCCGGGTATAAGGTCGGCGTGGTGAAGCAGACCGAAACCGCCGCCATTAAGGCACATGGGTCGAACCGAGCCGGTCCATTTGACCGTGGATTGTCGGCATTGTACACGAAGGCGACTCTGGAGGCGGCGGAGGATGTGGGGGGAAAAGAGGAGGGCTGTGGTGGGGATAGTAATTATTTGGCTTGTGTTGTGGACAAGAGCGTTTCAGTGGAAAATGTGGATGGTGGAGTTGAGAGCGGTGTGGAAGTGAAAATTGGAATTGTGGCAGTGGAGGTTTCGACGGGCGACGTTGTCTATGGAGAGTTTGATGATAATTTTATGAGGAGTGGGCTTGAGGCTGTGGTTTTGAGCTTGTCACCTGCTGAGTTGCTTCTTGGGGAGCCACTCTCCAAACAAACAGATAAGATGTTACTTGCTTTTTCTGGACCGGCTTCAAATGTCCGCGTGGAGCGTGTCTCACGAGATTGCTTCAAGGACAGTGGTGCTTTCGCTGAAGTAATGTCTTTATATGAAAACATGGAGGGTGGTGATTTAACAGATCATTCAAAGGTAAATACAGATGTGAAAGAACAGAGTAATAAGCACTTGGGAATTGAGGGATTCATGAACATGCCAAATATGGCAGTCCAAGCATTGGCCCTGACTATTTATCATCTGAAACAATTTGGTTTGCAAAGGATCCTGCGCCTAGGAGCTTCTTTTAGGCCCCTCTCAAGCAGCATGGAGATGACTCTCTCGGCCAATGCACTTCAGCAATTGGAGGTATTGAAGAACAATGCTGATGGATCCGAGTCTGGCTCCTTGCTGCAGTCTATGAATCATACTCTTACCATATTTGGTTCAAGGCTTCTTAGACACTGGGTATCTCACCCTTTATGTGATCGAACCATGATTTCTGCTCGTCTAGATGCTGTTTCTGAGATTGCAGAATCTATGGGGTCTTCTATATCTCCTCACAATATTGAACAGCTAGATGTGGAAGATTCGTTTGCAACAAATGTGAACCCAGAGCTGACTTATATACTTTCTTCAGTTCTGACGACTTTGGGACGGTCGCCTGATATTCAACGTGGGATAACAAGAATCTTTCATCGGACTGCCACTCCACCTGAGTTCATTGCAGTTATTCAAGCTATTTTATATGCTGGAAAACAACTTCAACAACTTCAAATTAAAGAAGAGGGAAGCAAAGGAAATACGAGGGGAAAAAGTGTACGCTCTGAGTTGTTGAGGAAGTTGATATTGACTGCTTCGTCGTCCACTGTAGTTGGAAAAGCTGCAAAATTGTTGTCTGCCCTCAACAAGGAAGCAGCTGACAAACAGGATCTACTAAACCTAATCATCTCTGATGGCCAATTTCCTGAGGTTGCTAAAGCAAGGAAGGAGGTTCAATTGGCAAATGAGAAATTGGATTCTCTCATCAGTTTGTACCGGAAACAGCTTGGAATGCGCAAGTTGGAATTCCTTAGCGTGTCTGGAACAACACACTTGATAGAGTTGACCTTAGATGTAAAGGTGCCCTCAAATTGGGTTAAGATTAATAGTACCAAAAAGACAGTCCGGTATCACCCACCTGAAGTCCTGACTGCTTTAGACCATCTAGCTCTTGCAAGTGAGCAACTCACTGTTGTTTGTCGTGCTGCTTGGGATAGCTTTCTAAGTGGTTTTGGTAAATATTATGCTGAGTTCCAAGCTGCTGTTCAAGCACTAGCCAGTTTAGACTGTCTTCATTCACTTGCTGTCCTTTCAAGAAATAAGAACTATGTTCGTCCAATGTTTGTATATGATGATGAACCGGTCCAGATACACATCTCCTCTGGTCGTCATCCGGTTTTGGAGACTATATTACAAGACAATTTTGTTCCCAATGATACAGATTTGGAGGCAGATGGGGAGTATTGTCAGATTATTACTGGACCCAATATGGGTGGAAAGAGTTGCTACATTCGCCAAGTTGCTCTCATTGCTATCATGGCTCAGGTTGGTTCCTTTGTTCCAGCATCATCGGCAAAACTGCATGTGCTGGACAGCATTTTCACTCGAATGGGTGCTTCTGACAGTATTCACCAAGGGAGAAGCACCTTTCTTGAAGAACTGAGCGAGGCTTCACATATACTTCACAATTGTACATCACGCTCATTGGTTATCATTGATGAGCTTGGGAGAGGCACGAGCACACACGATGGTGTGGCTATTGCTTATGCTACATTAAATCATCTACTACAGCAGAAAAGATGCATGGTCCTATTTGTCACGCACTACCCAAAAATTGCTCATATCAGAACTGAATTCCCAGGCTTAGTGGAGGCGTACCATGTTTCTTATCTGACGTCAAATAGAGATATGGGTTCGACAGGCATTCAATCCGAAAATCAAGATGTCACTTACTTATATAAGCTTGTGCCTGGTGTTTCAGAGAGGAGTTTCGGATTTAAGGTTGCAGAGCTTGCACAGCTACCTTCCTCATGCATCAGACGAGCGACTGTCATGGCTGCTAGGTTGGAGGCAGTAGTAAGCAGCCGAACAAGAAATAAGGATGACAAAAAGTGGTTGCTAAAATCACTGCCAACAGAACAAAAGCAGGAAGAGCAAGATGTGATGCTGGAATCTCCCGAGTGCCTTCGTGTGGGATGGAGCTCGATTTTAGGGGACATAGATGGTGCCCTGTACGAGAAATTCTTTAAGAATTTGAAAACCACATTACTTGATGACAGCGACCCCATAAAAAGCGTTGAGAACTTGAATCACACAAGAAGTATTGCAAGAGAATTAGTAAGCAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_IV MutS_V

Protein Analysis

1123

Amino Acids

122.93

Weight (kDa)

6.93

Isoelectric Point (pI)

49.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	126 - 236	1.4e-25	MutS domain I
MutS_II	PF05188	275 - 335	4.5e-06	MutS domain II
MutS_III	PF05192	443 - 775	8.5e-35	MutS domain III
MutS_V	PF00488	830 - 1030	8.8e-71	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25540
fragaria_vesca	FvH4_7g29360
malus_domestica	MD07G1267600.v1.1
prunus_persica	Prupe.2G293300_v2.0.a1 Prupe.6G163500_v2.0.a1
pyrus_communis	pycom01g20970 pycom07g24520
rosa_chinensis	RchiOBHm_Chr1g0377121
rosa_laevigata	RLG00000026541
rosa_multiflora	Rmu_ssc0000155.1_g000018
rosa_roxburghii	Rroxscaffold_4G00281430
rosa_rugosa	Rorug01G0401600
rosa_samantha	Rh1AG419000 Rh1BG378100 Rh1CG392100 Rh1DG408800
rosa_wichuraiana	Rw1G036750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	382, 952
AasI	GACNNNNNNGTC	1 cut(s)	1837
AatII	GACGTC	1 cut(s)	2918
Acc16I	TGCGCA	1 cut(s)	2036
Acc36I	ACCTGC	2 cut(s)	382, 952
AccB1I	GGYRCC	3 cut(s)	584, 2101, 3245
AccB7I	CCANNNNNTGG	1 cut(s)	1219
AccBSI	CCGCTC	1 cut(s)	822
AccII	CGCG	2 cut(s)	180, 1029
AclWI	GGATC	6 cut(s)	525, 1258, 1271, 1365, 1378, 1917
AcoI	YGGCCR	2 cut(s)	1323, 1936
AcsI	RAATTY	5 cut(s)	299, 2045, 2873, 3260, 3271
AcuI	CTGAAG	4 cut(s)	1098, 1319, 1599, 2181
AcyI	GRCGYC	2 cut(s)	474, 2915
AfaI	GTAC	8 cut(s)	455, 689, 1800, 2015, 2131, 2713, 2897, 3254
AfiI	CCNNNNNNNGG	5 cut(s)	1219, 1888, 2112, 2510, 3211
AgeI	ACCGGT	2 cut(s)	456, 2380
AjiI	CACGTC	1 cut(s)	568
AjnI	CCWGG	2 cut(s)	2878, 2989
AjuI	GAANNNNNNNTTGG	4 cut(s)	822, 854, 2268, 2300
AleI	CACNNNNGTG	1 cut(s)	2769
Alw21I	GWGCWC	2 cut(s)	2762, 3224
Alw26I	GTCTC	3 cut(s)	211, 1045, 2417
AlwI	GGATC	6 cut(s)	525, 1258, 1271, 1365, 1378, 1917
Ama87I	CYCGRG	1 cut(s)	3197
AoxI	GGCC	4 cut(s)	1221, 1289, 1323, 1936
ApoI	RAATTY	5 cut(s)	299, 2045, 2873, 3260, 3271
ArsI	GACNNNNNNTTYG	4 cut(s)	1859, 1891, 2127, 2159
AseI	ATTAAT	1 cut(s)	2124
AsiGI	ACCGGT	2 cut(s)	456, 2380
AspA2I	CCTAGG	1 cut(s)	1273
AspLEI	GCGC	4 cut(s)	182, 414, 1272, 2037
AspS9I	GGNCC	7 cut(s)	659, 1010, 1222, 1290, 2383, 2501, 2827
AsuC2I	CCSGG	2 cut(s)	419, 589
AsuHPI	GGTGA	6 cut(s)	619, 933, 1124, 1445, 2144, 2642
AvaI	CYCGRG	1 cut(s)	3197
AvaII	GGWCC	5 cut(s)	659, 1010, 2383, 2501, 2827
AvrII	CCTAGG	1 cut(s)	1273
AxyI	CCTNAGG	1 cut(s)	1947
BaeGI	GKGCMC	2 cut(s)	2106, 3250
BalI	TGGCCA	1 cut(s)	1938
BamHI	GGATCC	2 cut(s)	1263, 1370
BanI	GGYRCC	3 cut(s)	584, 2101, 3245
BanII	GRGCYC	1 cut(s)	3224
BauI	CACGAG	2 cut(s)	1044, 2755
BbsI	GAAGAC	3 cut(s)	477, 1518, 2303
Bbv12I	GWGCWC	2 cut(s)	2762, 3224
BccI	CCATC	9 cut(s)	427, 800, 1361, 1928, 2190, 2468, 2762, 3210, 3236
BceAI	ACGGC	2 cut(s)	91, 188
BcgI	CGANNNNNNTGC	4 cut(s)	467, 501, 627, 661
BciT130I	CCWGG	2 cut(s)	2880, 2991
BcnI	CCSGG	2 cut(s)	419, 589
BcoDI	GTCTC	3 cut(s)	211, 1045, 2417
BfaI	CTAG	7 cut(s)	317, 1274, 1490, 1556, 2186, 2294, 3081
BfmI	CTRYAG	3 cut(s)	1391, 1848, 2807
BfoI	RGCGCY	1 cut(s)	415
BfuAI	ACCTGC	2 cut(s)	382, 952
BglI	GCCNNNNNGGC	1 cut(s)	635
BlnI	CCTAGG	1 cut(s)	1273
Bme1390I	CCNGG	4 cut(s)	419, 589, 2880, 2991
Bme18I	GGWCC	5 cut(s)	659, 1010, 2383, 2501, 2827
BmeT110I	CYCGRG	1 cut(s)	3197
BmgBI	CACGTC	1 cut(s)	568
BmgT120I	GGNCC	7 cut(s)	659, 1010, 1222, 1290, 2383, 2501, 2827
BmrFI	CCNGG	4 cut(s)	419, 589, 2880, 2991
BmrI	ACTGGG	1 cut(s)	1453
BmsI	GCATC	5 cut(s)	1483, 2594, 2810, 3063, 3174
BmuI	ACTGGG	1 cut(s)	1453
BpiI	GAAGAC	3 cut(s)	477, 1518, 2303
BplI	GAGNNNNNCTC	6 cut(s)	956, 988, 1025, 1057, 1367, 1399
BpmI	CTGGAG	2 cut(s)	290, 725
Bpu10I	CCTNAGC	2 cut(s)	2051, 2562
BpuEI	CTTGAG	2 cut(s)	938, 1282
BpuMI	CCSGG	2 cut(s)	419, 589
BsaBI	GATNNNNATC	1 cut(s)	2393
BsaHI	GRCGYC	2 cut(s)	474, 2915
BsaJI	CCNNGG	4 cut(s)	670, 1273, 2653, 2878
BsaWI	WCCGGW	6 cut(s)	456, 552, 2016, 2145, 2380, 2413
BsaXI	ACNNNNNCTCC	4 cut(s)	121, 151, 1672, 1702
Bsc4I	CCNNNNNNNGG	5 cut(s)	1219, 1888, 2112, 2510, 3211
Bse118I	RCCGGY	4 cut(s)	456, 656, 1012, 2380
Bse1I	ACTGG	3 cut(s)	1448, 2298, 2503
Bse21I	CCTNAGG	1 cut(s)	1947
Bse3DI	GCAATG	2 cut(s)	1701, 2547
Bse8I	GATNNNNATC	1 cut(s)	2393
BseBI	CCWGG	2 cut(s)	2880, 2991
BseDI	CCNNGG	4 cut(s)	670, 1273, 2653, 2878
BseGI	GGATG	6 cut(s)	427, 724, 811, 2410, 3127, 3221
BseJI	GATNNNNATC	1 cut(s)	2393
BseLI	CCNNNNNNNGG	5 cut(s)	1219, 1888, 2112, 2510, 3211
BseMI	GCAATG	2 cut(s)	1701, 2547
BseMII	CTCAG	8 cut(s)	939, 1494, 1686, 1797, 1938, 2259, 2576, 2672
BseNI	ACTGG	3 cut(s)	1448, 2298, 2503
BseRI	GAGGAG	9 cut(s)	70, 73, 181, 237, 749, 922, 1527, 2389, 3019
BseSI	GKGCMC	2 cut(s)	2106, 3250
Bsh1236I	CGCG	2 cut(s)	180, 1029
Bsh1285I	CGRYCG	1 cut(s)	1640
BshFI	GGCC	4 cut(s)	1223, 1291, 1325, 1938
BshNI	GGYRCC	3 cut(s)	584, 2101, 3245
BshTI	ACCGGT	2 cut(s)	456, 2380
BsiEI	CGRYCG	1 cut(s)	1640
BsiHKAI	GWGCWC	2 cut(s)	2762, 3224
BsiHKCI	CYCGRG	1 cut(s)	3197
BslFI	GGGAC	3 cut(s)	527, 1647, 3248
BslI	CCNNNNNNNGG	5 cut(s)	1219, 1888, 2112, 2510, 3211
BsmAI	GTCTC	3 cut(s)	211, 1045, 2417
BsmBI	CGTCTC	1 cut(s)	211
BsmFI	GGGAC	3 cut(s)	527, 1647, 3248
BsmI	GAATGC	2 cut(s)	2037, 2943
BsnI	GGCC	4 cut(s)	1223, 1291, 1325, 1938
BsoBI	CYCGRG	1 cut(s)	3197
Bsp1286I	GDGCHC	4 cut(s)	2106, 2762, 3224, 3250
Bsp1407I	TGTACA	2 cut(s)	687, 2711
Bsp143I	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
BspANI	GGCC	4 cut(s)	1223, 1291, 1325, 1938
BspCNI	CTCAG	8 cut(s)	940, 1495, 1687, 1798, 1939, 2260, 2575, 2673
BspFNI	CGCG	2 cut(s)	180, 1029
BspHI	TCATGA	1 cut(s)	1188
BspMAI	CTGCAG	1 cut(s)	1395
BspMI	ACCTGC	2 cut(s)	382, 952
BspPI	GGATC	6 cut(s)	525, 1258, 1271, 1365, 1378, 1917
BspQI	GCTCTTC	1 cut(s)	3165
BspT107I	GGYRCC	3 cut(s)	584, 2101, 3245
BsrBI	CCGCTC	1 cut(s)	822
BsrDI	GCAATG	2 cut(s)	1701, 2547
BsrFI	RCCGGY	4 cut(s)	456, 656, 1012, 2380
BsrGI	TGTACA	2 cut(s)	687, 2711
BsrI	ACTGG	3 cut(s)	1448, 2298, 2503
BssAI	RCCGGY	4 cut(s)	456, 656, 1012, 2380
BssECI	CCNNGG	4 cut(s)	670, 1273, 2653, 2878
BssMI	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
BssNI	GRCGYC	2 cut(s)	474, 2915
BssSI	CACGAG	2 cut(s)	1044, 2755
BssT1I	CCWWGG	2 cut(s)	1273, 2653
Bst2BI	CACGAG	2 cut(s)	1044, 2755
Bst2UI	CCWGG	2 cut(s)	2880, 2991
Bst6I	CTCTTC	3 cut(s)	214, 1758, 3165
BstACI	GRCGYC	2 cut(s)	474, 2915
BstAPI	GCANNNNNTGC	1 cut(s)	3032
BstAUI	TGTACA	2 cut(s)	687, 2711
BstC8I	GCNNGC	1 cut(s)	3033
BstDSI	CCRYGG	1 cut(s)	670
BstENI	CCTNNNNNAGG	1 cut(s)	1886
BstF5I	GGATG	6 cut(s)	427, 724, 811, 2410, 3127, 3221
BstFNI	CGCG	2 cut(s)	180, 1029
BstH2I	RGCGCY	1 cut(s)	415
BstHHI	GCGC	4 cut(s)	182, 414, 1272, 2037
BstKTI	GATC	6 cut(s)	520, 1126, 1266, 1373, 1468, 1912
BstMAI	GTCTC	3 cut(s)	211, 1045, 2417
BstMBI	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
BstMCI	CGRYCG	1 cut(s)	1640
BstNI	CCWGG	2 cut(s)	2880, 2991
BstNSI	RCATGY	2 cut(s)	1198, 2606
BstSCI	CCNGG	4 cut(s)	417, 587, 2878, 2989
BstSFI	CTRYAG	3 cut(s)	1391, 1848, 2807
BstSLI	GKGCMC	2 cut(s)	2106, 3250
BstUI	CGCG	2 cut(s)	180, 1029
BstV2I	GAAGAC	3 cut(s)	477, 1518, 2303
BstX2I	RGATCY	3 cut(s)	1263, 1370, 1909
BstYI	RGATCY	3 cut(s)	1263, 1370, 1909
Bsu36I	CCTNAGG	1 cut(s)	1947
BsuRI	GGCC	4 cut(s)	1223, 1291, 1325, 1938
BtgI	CCRYGG	1 cut(s)	670
BtgZI	GCGATG	1 cut(s)	84
BtrI	CACGTC	1 cut(s)	568
BtsCI	GGATG	6 cut(s)	427, 724, 811, 2410, 3127, 3221
BtsI	GCAGTG	2 cut(s)	858, 3146
BtsIMutI	CAGTG	7 cut(s)	798, 858, 1069, 1441, 1845, 2208, 3146
BveI	ACCTGC	2 cut(s)	382, 952
Cac8I	GCNNGC	1 cut(s)	3033
CciI	TCATGA	1 cut(s)	1188
CfoI	GCGC	4 cut(s)	182, 414, 1272, 2037
Cfr10I	RCCGGY	4 cut(s)	456, 656, 1012, 2380
Cfr13I	GGNCC	7 cut(s)	659, 1010, 1222, 1290, 2383, 2501, 2827
CseI	GACGC	1 cut(s)	482
Csp6I	GTAC	8 cut(s)	454, 688, 1799, 2014, 2130, 2712, 2896, 3253
CspAI	ACCGGT	2 cut(s)	456, 2380
CspCI	CAANNNNNGTGG	8 cut(s)	1045, 1080, 1834, 1869, 2494, 2529, 3271, 3306
CviQI	GTAC	8 cut(s)	454, 688, 1799, 2014, 2130, 2712, 2896, 3253
DpnI	GATC	6 cut(s)	519, 1125, 1265, 1372, 1467, 1911
DpnII	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
DrdI	GACNNNNNNGTC	1 cut(s)	1837
DseDI	GACNNNNNNGTC	1 cut(s)	1837
EaeI	YGGCCR	2 cut(s)	1323, 1936
Eam1104I	CTCTTC	3 cut(s)	214, 1758, 3165
EarI	CTCTTC	3 cut(s)	214, 1758, 3165
EciI	GGCGGA	1 cut(s)	728
Ecl136II	GAGCTC	1 cut(s)	3222
Eco130I	CCWWGG	2 cut(s)	1273, 2653
Eco24I	GRGCYC	1 cut(s)	3224
Eco47I	GGWCC	5 cut(s)	659, 1010, 2383, 2501, 2827
Eco53kI	GAGCTC	1 cut(s)	3222
Eco57I	CTGAAG	4 cut(s)	1098, 1319, 1599, 2181
Eco81I	CCTNAGG	1 cut(s)	1947
Eco88I	CYCGRG	1 cut(s)	3197
EcoICRI	GAGCTC	1 cut(s)	3222
EcoNI	CCTNNNNNAGG	1 cut(s)	1886
EcoO109I	RGGNCCY	1 cut(s)	1290
EcoRI	GAATTC	2 cut(s)	2045, 2873
EcoRII	CCWGG	2 cut(s)	2878, 2989
EcoT14I	CCWWGG	2 cut(s)	1273, 2653
EcoT22I	ATGCAT	2 cut(s)	2825, 3056
EcoT38I	GRGCYC	1 cut(s)	3224
ErhI	CCWWGG	2 cut(s)	1273, 2653
Esp3I	CGTCTC	1 cut(s)	211
FaqI	GGGAC	3 cut(s)	527, 1647, 3248
FauI	CCCGC	5 cut(s)	38, 178, 277, 331, 373
FokI	GGATG	6 cut(s)	434, 731, 818, 2397, 3134, 3228
FriOI	GRGCYC	1 cut(s)	3224
FspBI	CTAG	7 cut(s)	317, 1274, 1490, 1556, 2186, 2294, 3081
FspI	TGCGCA	1 cut(s)	2036
GlaI	GCGC	4 cut(s)	181, 413, 1271, 2036
GsuI	CTGGAG	2 cut(s)	290, 725
HaeII	RGCGCY	1 cut(s)	415
HaeIII	GGCC	4 cut(s)	1223, 1291, 1325, 1938
HgaI	GACGC	1 cut(s)	482
HhaI	GCGC	4 cut(s)	182, 414, 1272, 2037
Hin1I	GRCGYC	2 cut(s)	474, 2915
Hin6I	GCGC	4 cut(s)	180, 412, 1270, 2035
HinP1I	GCGC	4 cut(s)	180, 412, 1270, 2035
HincII	GTYRAC	1 cut(s)	2085
HindII	GTYRAC	1 cut(s)	2085
HindIII	AAGCTT	2 cut(s)	311, 2981
HphI	GGTGA	6 cut(s)	619, 933, 1124, 1445, 2144, 2642
Hpy166II	GTNNAC	8 cut(s)	375, 565, 690, 778, 1588, 1799, 1845, 2085
Hpy8I	GTNNAC	8 cut(s)	375, 565, 690, 778, 1588, 1799, 1845, 2085
Hpy99I	CGWCG	4 cut(s)	335, 868, 875, 1843
HpyAV	CCTTC	8 cut(s)	84, 172, 360, 559, 688, 1960, 3054, 3215
HpyCH4IV	ACGT	5 cut(s)	333, 567, 873, 1654, 2915
HpySE526I	ACGT	5 cut(s)	333, 567, 873, 1654, 2915
Hsp92I	GRCGYC	2 cut(s)	474, 2915
HspAI	GCGC	4 cut(s)	180, 412, 1270, 2035
Kzo9I	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
LguI	GCTCTTC	1 cut(s)	3165
LmnI	GCTCC	7 cut(s)	46, 385, 964, 1033, 1277, 1388, 3219
LweI	GCATC	5 cut(s)	1483, 2594, 2810, 3063, 3174
MaeI	CTAG	7 cut(s)	317, 1274, 1490, 1556, 2186, 2294, 3081
MaeII	ACGT	5 cut(s)	333, 567, 873, 1654, 2915
MaeIII	GTNAC	5 cut(s)	446, 939, 993, 2836, 2965
MalI	GATC	6 cut(s)	519, 1125, 1265, 1372, 1467, 1911
MbiI	CCGCTC	1 cut(s)	822
MboI	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
MfeI	CAATTG	3 cut(s)	1340, 1976, 2707
MflI	RGATCY	3 cut(s)	1263, 1370, 1909
MhlI	GDGCHC	4 cut(s)	2106, 2762, 3224, 3250
MlsI	TGGCCA	1 cut(s)	1938
MluNI	TGGCCA	1 cut(s)	1938
MlyI	GAGTC	3 cut(s)	694, 1309, 1385
MmeI	TCCRAC	5 cut(s)	354, 381, 1834, 2022, 3066
Mox20I	TGGCCA	1 cut(s)	1938
Mph1103I	ATGCAT	2 cut(s)	2825, 3056
MscI	TGGCCA	1 cut(s)	1938
MseI	TTAA	8 cut(s)	633, 1118, 1758, 2118, 2124, 2795, 3018, 3267
MslI	CAYNNNNRTG	5 cut(s)	420, 1193, 1310, 2627, 2769
Msp20I	TGGCCA	1 cut(s)	1938
MspA1I	CMGCKG	1 cut(s)	1898
MspR9I	CCNGG	4 cut(s)	419, 589, 2880, 2991
MunI	CAATTG	3 cut(s)	1340, 1976, 2707
Mva1269I	GAATGC	2 cut(s)	2037, 2943
MvaI	CCWGG	2 cut(s)	2880, 2991
MvnI	CGCG	2 cut(s)	180, 1029
NciI	CCSGG	2 cut(s)	419, 589
NdeII	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
NmeAIII	GCCGAG	1 cut(s)	1301
NmuCI	GTSAC	3 cut(s)	939, 2836, 2965
NsbI	TGCGCA	1 cut(s)	2036
NsiI	ATGCAT	2 cut(s)	2825, 3056
NspI	RCATGY	2 cut(s)	1198, 2606
OliI	CACNNNNGTG	1 cut(s)	2769
PagI	TCATGA	1 cut(s)	1188
PaqCI	CACCTGC	2 cut(s)	382, 952
PciSI	GCTCTTC	1 cut(s)	3165
PctI	GAATGC	2 cut(s)	2037, 2943
PfeI	GAWTC	8 cut(s)	1185, 1402, 1514, 1568, 1668, 1997, 3191, 3328
PflFI	GACNNNGTC	1 cut(s)	875
PflMI	CCANNNNNTGG	1 cut(s)	1219
PinAI	ACCGGT	2 cut(s)	456, 2380
PleI	GAGTC	3 cut(s)	694, 1309, 1384
PpsI	GAGTC	3 cut(s)	694, 1309, 1384
PshBI	ATTAAT	1 cut(s)	2124
Psp124BI	GAGCTC	1 cut(s)	3224
Psp6I	CCWGG	2 cut(s)	2878, 2989
PspGI	CCWGG	2 cut(s)	2878, 2989
PspPI	GGNCC	7 cut(s)	659, 1010, 1222, 1290, 2383, 2501, 2827
PstI	CTGCAG	1 cut(s)	1395
PsuI	RGATCY	3 cut(s)	1263, 1370, 1909
PsyI	GACNNNGTC	1 cut(s)	875
PvuII	CAGCTG	1 cut(s)	1898
RsaI	GTAC	8 cut(s)	455, 689, 1800, 2015, 2131, 2713, 2897, 3254
RsaNI	GTAC	8 cut(s)	454, 688, 1799, 2014, 2130, 2712, 2896, 3253
RseI	CAYNNNNRTG	5 cut(s)	420, 1193, 1310, 2627, 2769
SacI	GAGCTC	1 cut(s)	3224
SapI	GCTCTTC	1 cut(s)	3165
SaqAI	TTAA	8 cut(s)	633, 1118, 1758, 2118, 2124, 2795, 3018, 3267
Sau3AI	GATC	6 cut(s)	517, 1123, 1263, 1370, 1465, 1909
Sau96I	GGNCC	7 cut(s)	659, 1010, 1222, 1290, 2383, 2501, 2827
SchI	GAGTC	3 cut(s)	694, 1309, 1385
ScrFI	CCNGG	4 cut(s)	419, 589, 2880, 2991
SduI	GDGCHC	4 cut(s)	2106, 2762, 3224, 3250
SfaNI	GCATC	5 cut(s)	1483, 2594, 2810, 3063, 3174
SfcI	CTRYAG	3 cut(s)	1391, 1848, 2807
SinI	GGWCC	5 cut(s)	659, 1010, 2383, 2501, 2827
SmiMI	CAYNNNNRTG	5 cut(s)	420, 1193, 1310, 2627, 2769
SmlI	CTYRAG	2 cut(s)	917, 1297
SmoI	CTYRAG	2 cut(s)	917, 1297
SspI	AATATT	2 cut(s)	1546, 2261
SspMI	CTAG	7 cut(s)	317, 1274, 1490, 1556, 2186, 2294, 3081
SstI	GAGCTC	1 cut(s)	3224
StyD4I	CCNGG	4 cut(s)	417, 587, 2878, 2989
StyI	CCWWGG	2 cut(s)	1273, 2653
TaiI	ACGT	5 cut(s)	336, 570, 876, 1657, 2918
TaqI	TCGA	6 cut(s)	647, 863, 1468, 2626, 2936, 3224
TaqII	GACCGA	1 cut(s)	632
TatI	WGTACW	2 cut(s)	687, 2711
TauI	GCSGC	4 cut(s)	106, 275, 629, 713
TfiI	GAWTC	8 cut(s)	1185, 1402, 1514, 1568, 1668, 1997, 3191, 3328
Tru1I	TTAA	8 cut(s)	633, 1118, 1758, 2118, 2124, 2795, 3018, 3267
Tru9I	TTAA	8 cut(s)	633, 1118, 1758, 2118, 2124, 2795, 3018, 3267
TscAI	CASTG	7 cut(s)	798, 858, 1069, 1448, 1852, 2215, 3153
TseFI	GTSAC	3 cut(s)	939, 2836, 2965
Tsp45I	GTSAC	3 cut(s)	939, 2836, 2965
TspDTI	ATGAA	8 cut(s)	1110, 1177, 1205, 1415, 1664, 1690, 2303, 2391
TspRI	CASTG	7 cut(s)	798, 858, 1069, 1448, 1852, 2215, 3153
Tth111I	GACNNNGTC	1 cut(s)	875
Van91I	CCANNNNNTGG	1 cut(s)	1219
VpaK11BI	GGWCC	5 cut(s)	659, 1010, 2383, 2501, 2827
VspI	ATTAAT	1 cut(s)	2124
XagI	CCTNNNNNAGG	1 cut(s)	1886
XapI	RAATTY	5 cut(s)	299, 2045, 2873, 3260, 3271
XbaI	TCTAGA	2 cut(s)	316, 1489
XceI	RCATGY	2 cut(s)	1198, 2606
XmaJI	CCTAGG	1 cut(s)	1273
XspI	CTAG	7 cut(s)	317, 1274, 1490, 1556, 2186, 2294, 3081
ZraI	GACGTC	1 cut(s)	2916
Zsp2I	ATGCAT	2 cut(s)	2825, 3056

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.