MD07G1296700.v1.1

Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012990
Physical Location & Seq
Reverse (-)
35692198 .. 35694549
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1296700.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGACTGTCGGTGCCCGGTCTGCCATTTTCTCAGCCAGTAAGCCCGGGTGCGCAGTGCGCATGGCCTCCATATGCTCCCCGACCCGTCTTTCCCTCTCACTCCGCACGACTCTCTCCTCATTGCGTACATCTCGCGCGCCGGGAATCTCACTCCGATCGTCACCAATATCTCTATCCCTCTCGATTAAACCCGTTCGCTCGAATTTCAAGAACCCAGTTTCCGCATCATCGGCGTCAGCAGATATGGCGGGTCAGTGTCAGAAGAAGGTGGAGAAGTTTCAGACGGAGGAGGAAGTGGCGGTGCGTCTGGCGAAGTACACTGCCGACCTCTCGGCCAAGTTTGAGAAGGAGAGGGGAGCCTTCACCGTCGTTTTGTCCGGCGGCTCTCTCATTTACACGCTCAGGAAATTAGCGGAAGAGCCGTATATGTATATGGTGGAATGGGAGAAATGGCATGTGTTCTGGGTGGATGAGAGAGTGGTGAAAAGAGATCACGCCGACAGTAACTATAAACTTGCTCTTGAAGGTTTACTCTGCAAGGTACCGATTCCTCCTGGCCAGGTTTACGCCATTAACGACACACTTTCAGCCGAGGGAGCAGCTGAGGATTACGAAACCTGTCTTAAACATTTGGTTGAGCGCAATGTGATTGCCACTTCAAAAGCTACTGGATTTCCAAAGTTTGATCTCATGCTTCTGGGTATGGGGCCAGATGGACATCTTGCTTCTCTATTCCCTGGCCATCCGCTTTGCAACGAGAAGACGAAATGGGTGACTCACATTACGGACTCGCCAAAACCACCTCCAGAGAGGATTACATATACCTTCCCGGTGATCAACTCCGCTGCGTATAATGCAATGGTGGTGACAGGTGACGATAAAGCTGATGCCGTGCAGAGAGCGCTAGGAAATAGTCAGCATCCTGATACTTTGCCTGTTCAGTCGCTTGCAGCCGAAGACGAGTTAACTTGGTTTTTAGACCAGGGTGCAGCGTCCAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko01100 ko01110 ko01120 ko01130 ko01200 map00030 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
Glucosamine_iso

Protein Analysis

335

Amino Acids

36.48

Weight (kDa)

8.13

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucosamine_iso PF01182 95 - 324 6.5e-70 Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012990)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49360 AT5G24400
fragaria_vesca FvH4_7g32550
malus_domestica MD01G1226300.v1.1 MD07G1296700.v1.1
prunus_persica Prupe.2G316300_v2.0.a1
pyrus_communis pycom01g23480 pycom07g26940
rosa_chinensis RchiOBHm_Chr1g0382391
rosa_laevigata RLG00000026181
rosa_multiflora Rmu_sc0000110.1_g000044
rosa_roxburghii Rroxscaffold_4G00278140
rosa_rugosa Rorug01G0434000
rosa_samantha Rh1AG458700 Rh1BG415000 Rh1CG429100 Rh1DG447100
rosa_wichuraiana Rw1G041230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 52, 59
Acc65I GGTACC 1 cut(s) 541
AccB1I GGYRCC 2 cut(s) 11, 541
AccII CGCG 2 cut(s) 135, 137
AciI CCGC 8 cut(s) 103, 222, 248, 299, 381, 413, 746, 843
AcoI YGGCCR 3 cut(s) 333, 556, 739
AcsI RAATTY 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 3 cut(s) 127, 317, 543
AfeI AGCGCT 1 cut(s) 903
AgsI TTSAA 3 cut(s) 208, 524, 660
AjnI CCWGG 4 cut(s) 553, 558, 736, 981
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
AluBI AGCT 4 cut(s) 602, 665, 884, 1000
AluI AGCT 4 cut(s) 602, 665, 884, 1000
Ama87I CYCGRG 1 cut(s) 44
Aor51HI AGCGCT 1 cut(s) 903
AoxI GGCC 5 cut(s) 63, 333, 556, 707, 739
ApeKI GCWGC 4 cut(s) 599, 845, 950, 989
ApoI RAATTY 1 cut(s) 202
Asp718I GGTACC 1 cut(s) 541
AspLEI GCGC 6 cut(s) 53, 60, 137, 139, 642, 904
AspS9I GGNCC 1 cut(s) 707
AsuC2I CCSGG 5 cut(s) 16, 45, 46, 141, 830
AsuHPI GGTGA 7 cut(s) 153, 355, 493, 784, 844, 877, 884
AvaI CYCGRG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 16
BalI TGGCCA 2 cut(s) 558, 741
BanI GGYRCC 2 cut(s) 11, 541
BbsI GAAGAC 2 cut(s) 767, 963
BbvCI CCTCAGC 1 cut(s) 603
BbvI GCAGC 4 cut(s) 611, 832, 962, 1001
BccI CCATC 2 cut(s) 707, 750
BceAI ACGGC 2 cut(s) 406, 875
BciT130I CCWGG 4 cut(s) 555, 560, 738, 983
BclI TGATCA 1 cut(s) 834
BcnI CCSGG 5 cut(s) 16, 45, 46, 141, 830
BfaI CTAG 1 cut(s) 905
BfmI CTRYAG 1 cut(s) 1001
BfoI RGCGCY 1 cut(s) 905
BisI GCNGC 5 cut(s) 382, 600, 846, 951, 990
BlsI GCNGC 5 cut(s) 383, 601, 847, 952, 991
Bme1390I CCNGG 9 cut(s) 16, 45, 46, 141, 555, 560, 738, 830, 983
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 707
BmiI GGNNCC 4 cut(s) 13, 358, 543, 708
BmrFI CCNGG 9 cut(s) 16, 45, 46, 141, 555, 560, 738, 830, 983
BmrI ACTGGG 1 cut(s) 209
BmsI GCATC 3 cut(s) 233, 877, 928
BmuI ACTGGG 1 cut(s) 209
BpiI GAAGAC 2 cut(s) 767, 963
BpmI CTGGAG 1 cut(s) 789
Bpu10I CCTNAGC 2 cut(s) 401, 603
BpuMI CCSGG 5 cut(s) 16, 45, 46, 141, 830
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 4 cut(s) 44, 591, 736, 982
Bse1I ACTGG 3 cut(s) 36, 215, 673
Bse3DI GCAATG 3 cut(s) 119, 649, 864
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 4 cut(s) 555, 560, 738, 983
BseDI CCNNGG 4 cut(s) 44, 591, 736, 982
BseGI GGATG 3 cut(s) 475, 742, 919
BseJI GATNNNNATC 1 cut(s) 717
BseMI GCAATG 3 cut(s) 119, 649, 864
BseMII CTCAG 3 cut(s) 45, 415, 594
BseNI ACTGG 3 cut(s) 36, 215, 673
BsePI GCGCGC 1 cut(s) 135
BseRI GAGGAG 2 cut(s) 106, 302
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 4 cut(s) 611, 832, 962, 1001
BsgI GTGCAG 1 cut(s) 914
Bsh1236I CGCG 2 cut(s) 135, 137
Bsh1285I CGRYCG 1 cut(s) 158
BshFI GGCC 5 cut(s) 65, 335, 558, 709, 741
BshNI GGYRCC 2 cut(s) 11, 541
BsiEI CGRYCG 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 5 cut(s) 16, 45, 140, 378, 830
BsnI GGCC 5 cut(s) 65, 335, 558, 709, 741
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 4 cut(s) 155, 490, 685, 834
BspACI CCGC 8 cut(s) 103, 222, 248, 299, 381, 413, 746, 843
BspANI GGCC 5 cut(s) 65, 335, 558, 709, 741
BspCNI CTCAG 3 cut(s) 44, 414, 595
BspFNI CGCG 2 cut(s) 135, 137
BspLI GGNNCC 4 cut(s) 13, 358, 543, 708
BspQI GCTCTTC 1 cut(s) 411
BspT107I GGYRCC 2 cut(s) 11, 541
BsrDI GCAATG 3 cut(s) 119, 649, 864
BsrI ACTGG 3 cut(s) 36, 215, 673
BssECI CCNNGG 4 cut(s) 44, 591, 736, 982
BssHII GCGCGC 1 cut(s) 135
BssMI GATC 4 cut(s) 155, 490, 685, 834
BssNI GRCGYC 1 cut(s) 233
Bst2UI CCWGG 4 cut(s) 555, 560, 738, 983
Bst4CI ACNGT 3 cut(s) 7, 367, 503
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 137, 948
BstDEI CTNAG 3 cut(s) 31, 401, 603
BstF5I GGATG 3 cut(s) 475, 742, 919
BstFNI CGCG 2 cut(s) 135, 137
BstH2I RGCGCY 1 cut(s) 905
BstHHI GCGC 6 cut(s) 53, 60, 137, 139, 642, 904
BstKTI GATC 4 cut(s) 158, 493, 688, 837
BstMBI GATC 4 cut(s) 155, 490, 685, 834
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 7 cut(s) 20, 57, 230, 245, 596, 854, 901
BstNI CCWGG 4 cut(s) 555, 560, 738, 983
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 9 cut(s) 14, 43, 44, 139, 553, 558, 736, 828, 981
BstSFI CTRYAG 1 cut(s) 1001
BstSLI GKGCMC 1 cut(s) 16
BstUI CGCG 2 cut(s) 135, 137
BstV1I GCAGC 4 cut(s) 611, 832, 962, 1001
BstV2I GAAGAC 2 cut(s) 767, 963
BsuRI GGCC 5 cut(s) 65, 335, 558, 709, 741
BtsCI GGATG 3 cut(s) 475, 742, 919
BtsI GCAGTG 2 cut(s) 60, 318
BtsIMutI CAGTG 3 cut(s) 60, 260, 318
Cac8I GCNNGC 2 cut(s) 137, 948
CfoI GCGC 6 cut(s) 53, 60, 137, 139, 642, 904
Cfr13I GGNCC 1 cut(s) 707
Cfr9I CCCGGG 1 cut(s) 44
CseI GACGC 3 cut(s) 222, 293, 981
Csp6I GTAC 3 cut(s) 126, 316, 542
CviAII CATG 3 cut(s) 61, 455, 691
CviQI GTAC 3 cut(s) 126, 316, 542
DdeI CTNAG 3 cut(s) 31, 401, 603
DpnI GATC 4 cut(s) 157, 492, 687, 836
DpnII GATC 4 cut(s) 155, 490, 685, 834
EaeI YGGCCR 3 cut(s) 333, 556, 739
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco47III AGCGCT 1 cut(s) 903
Eco88I CYCGRG 1 cut(s) 44
EcoRII CCWGG 4 cut(s) 553, 558, 736, 981
FaeI CATG 3 cut(s) 64, 458, 694
FatI CATG 3 cut(s) 60, 454, 690
FauI CCCGC 1 cut(s) 241
FauNDI CATATG 1 cut(s) 71
FbaI TGATCA 1 cut(s) 834
Fnu4HI GCNGC 5 cut(s) 382, 600, 846, 951, 990
FokI GGATG 3 cut(s) 482, 729, 906
Fsp4HI GCNGC 5 cut(s) 382, 600, 846, 951, 990
FspAI RTGCGCAY 1 cut(s) 59
FspBI CTAG 1 cut(s) 905
FspI TGCGCA 2 cut(s) 52, 59
GlaI GCGC 6 cut(s) 52, 59, 136, 138, 641, 903
GluI GCNGC 5 cut(s) 382, 600, 846, 951, 990
GsuI CTGGAG 1 cut(s) 789
HaeII RGCGCY 1 cut(s) 905
HaeIII GGCC 5 cut(s) 65, 335, 558, 709, 741
HapII CCGG 5 cut(s) 16, 45, 140, 378, 830
HgaI GACGC 3 cut(s) 222, 293, 981
HhaI GCGC 6 cut(s) 53, 60, 137, 139, 642, 904
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 3 cut(s) 64, 458, 694
Hin6I GCGC 6 cut(s) 51, 58, 135, 137, 640, 902
HinP1I GCGC 6 cut(s) 51, 58, 135, 137, 640, 902
HincII GTYRAC 1 cut(s) 966
HindII GTYRAC 1 cut(s) 966
HinfI GANTC 5 cut(s) 109, 144, 547, 775, 788
HpaI GTTAAC 1 cut(s) 966
HpaII CCGG 5 cut(s) 16, 45, 140, 378, 830
HphI GGTGA 7 cut(s) 153, 355, 493, 784, 844, 877, 884
Hpy166II GTNNAC 4 cut(s) 318, 530, 565, 966
Hpy188I TCNGA 3 cut(s) 155, 261, 282
Hpy188III TCNNGA 6 cut(s) 181, 208, 403, 521, 806, 923
Hpy8I GTNNAC 4 cut(s) 318, 530, 565, 966
Hpy99I CGWCG 1 cut(s) 371
HpyAV CCTTC 5 cut(s) 259, 340, 370, 518, 835
HpyCH4III ACNGT 3 cut(s) 7, 367, 503
HpyCH4V TGCA 6 cut(s) 537, 753, 857, 895, 950, 989
HpyF10VI GCNNNNNNNGC 7 cut(s) 20, 57, 230, 245, 596, 854, 901
HpyF3I CTNAG 3 cut(s) 31, 401, 603
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 3 cut(s) 64, 458, 694
HspAI GCGC 6 cut(s) 51, 58, 135, 137, 640, 902
KpnI GGTACC 1 cut(s) 545
Ksp22I TGATCA 1 cut(s) 834
KspAI GTTAAC 1 cut(s) 966
Kzo9I GATC 4 cut(s) 155, 490, 685, 834
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 3 cut(s) 80, 356, 596
Lsp1109I GCAGC 4 cut(s) 611, 832, 962, 1001
LweI GCATC 3 cut(s) 233, 877, 928
MaeI CTAG 1 cut(s) 905
MaeIII GTNAC 5 cut(s) 159, 503, 772, 865, 872
MalI GATC 4 cut(s) 157, 492, 687, 836
MboI GATC 4 cut(s) 155, 490, 685, 834
MboII GAAGA 4 cut(s) 274, 428, 772, 968
MhlI GDGCHC 1 cut(s) 16
MlsI TGGCCA 2 cut(s) 558, 741
MluCI AATT 2 cut(s) 202, 407
MluNI TGGCCA 2 cut(s) 558, 741
MlyI GAGTC 3 cut(s) 103, 769, 782
Mox20I TGGCCA 2 cut(s) 558, 741
MscI TGGCCA 2 cut(s) 558, 741
MseI TTAA 4 cut(s) 186, 573, 624, 965
Msp20I TGGCCA 2 cut(s) 558, 741
MspA1I CMGCKG 2 cut(s) 602, 845
MspI CCGG 5 cut(s) 16, 45, 140, 378, 830
MspR9I CCNGG 9 cut(s) 16, 45, 46, 141, 555, 560, 738, 830, 983
MvaI CCWGG 4 cut(s) 555, 560, 738, 983
MvnI CGCG 2 cut(s) 135, 137
MwoI GCNNNNNNNGC 7 cut(s) 20, 57, 230, 245, 596, 854, 901
NciI CCSGG 5 cut(s) 16, 45, 46, 141, 830
NdeI CATATG 1 cut(s) 71
NdeII GATC 4 cut(s) 155, 490, 685, 834
NlaIII CATG 3 cut(s) 64, 458, 694
NlaIV GGNNCC 4 cut(s) 13, 358, 543, 708
NmeAIII GCCGAG 2 cut(s) 311, 616
NmuCI GTSAC 4 cut(s) 159, 772, 865, 872
NsbI TGCGCA 2 cut(s) 52, 59
NspI RCATGY 1 cut(s) 458
PauI GCGCGC 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PfeI GAWTC 2 cut(s) 144, 547
PkrI GCNGC 5 cut(s) 383, 601, 847, 952, 991
Ple19I CGATCG 1 cut(s) 158
PleI GAGTC 3 cut(s) 103, 769, 782
PpsI GAGTC 3 cut(s) 103, 769, 782
Psp6I CCWGG 4 cut(s) 553, 558, 736, 981
PspGI CCWGG 4 cut(s) 553, 558, 736, 981
PspN4I GGNNCC 4 cut(s) 13, 358, 543, 708
PspPI GGNCC 1 cut(s) 707
PteI GCGCGC 1 cut(s) 135
PvuI CGATCG 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 3 cut(s) 127, 317, 543
RsaNI GTAC 3 cut(s) 126, 316, 542
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 4 cut(s) 186, 573, 624, 965
SatI GCNGC 5 cut(s) 382, 600, 846, 951, 990
Sau3AI GATC 4 cut(s) 155, 490, 685, 834
Sau96I GGNCC 1 cut(s) 707
SchI GAGTC 3 cut(s) 103, 769, 782
ScrFI CCNGG 9 cut(s) 16, 45, 46, 141, 555, 560, 738, 830, 983
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 3 cut(s) 233, 877, 928
SfcI CTRYAG 1 cut(s) 1001
SmaI CCCGGG 1 cut(s) 46
Sse9I AATT 2 cut(s) 202, 407
SsiI CCGC 8 cut(s) 103, 222, 248, 299, 381, 413, 746, 843
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 9 cut(s) 14, 43, 44, 139, 553, 558, 736, 828, 981
TaaI ACNGT 3 cut(s) 7, 367, 503
TaqI TCGA 2 cut(s) 182, 200
TasI AATT 2 cut(s) 202, 407
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 2 cut(s) 144, 547
Tru1I TTAA 4 cut(s) 186, 573, 624, 965
Tru9I TTAA 4 cut(s) 186, 573, 624, 965
TscAI CASTG 3 cut(s) 60, 260, 325
TseFI GTSAC 4 cut(s) 159, 772, 865, 872
TseI GCWGC 4 cut(s) 599, 845, 950, 989
Tsp45I GTSAC 4 cut(s) 159, 772, 865, 872
TspGWI ACGGA 2 cut(s) 299, 800
TspMI CCCGGG 1 cut(s) 44
TspRI CASTG 3 cut(s) 60, 260, 325
XapI RAATTY 1 cut(s) 202
XceI RCATGY 1 cut(s) 458
XmaI CCCGGG 1 cut(s) 44
XspI CTAG 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.