pycom01g23480

Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012990
Physical Location & Seq
Reverse (-)
20776828 .. 20782028
5201 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23480.1

Sequence Viewer

Length: 762 bp
ATGGCGGACCAGTGTGAGAAGAAGGTGGAGAAGTTTCAGACGGAGGAGGAAGTGGCGGTGCATCTGGCGAAGTACACCGCCGACCTGTCGGCCAAGTTTGTGAAGGAGAGGGGAGTTTTCACCGTCGTCTTGTCCGGCGGCTCTCTCATTTACTCGCTCAGGAAATTAGCGGAAGAGCCGTATAAGGATACGGTGGAGTGGGGGAAATGGCATGTGTTCTGGCTGGACGAGAGAGTGGTGAAGAAGGATCACCCCGACAGTAACTATAAACTTGCTTATGATGGTTTACTCTGTAAGGTACCGATTCCCCCTGGTCAGGTTTATGCCATTAATGATGCACTTTCGGCTGAGGGAGCAGCAGAAGATTATGAAACCTGTCTTAAACATTTGGTTCAGCGCAATGTGATTGCCACTTCAAAAGTTACTGGATTTCCGAAGTTTGATCTCATACTTCTGGGTATGGGTCCAGATGGACATCTTGCTTCTCTATTCCCTGGTCATCCGCGTTGCAACGAGAAGACAAAATGGGTGACTCACATCGAGGACTCACCAAAACCACCTCCAGAGAGGATTACATTTACCTTCCCAGTGATCAACTCTGCTGCATATAATGCAATGGTGGTGGCAGGCGATAATAAAGCTGATGCCGTGCAGAGAGCACTGGGAAACAGTCAACATCCTGATACTTTGCCTGTTCAGTTGCTTGCGGCTGAAGACGAGTTAACTTGGTTCTTAGACCAGGGTGCAGCTTCCAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko01100 ko01110 ko01120 ko01130 ko01200 map00030 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
Glucosamine_iso

Protein Analysis

254

Amino Acids

28.09

Weight (kDa)

5.64

Isoelectric Point (pI)

30.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucosamine_iso PF01182 14 - 243 6.4e-72 Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012990)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49360 AT5G24400
fragaria_vesca FvH4_7g32550
malus_domestica MD01G1226300.v1.1 MD07G1296700.v1.1
prunus_persica Prupe.2G316300_v2.0.a1
pyrus_communis pycom01g23480 pycom07g26940
rosa_chinensis RchiOBHm_Chr1g0382391
rosa_laevigata RLG00000026181
rosa_multiflora Rmu_sc0000110.1_g000044
rosa_roxburghii Rroxscaffold_4G00278140
rosa_rugosa Rorug01G0434000
rosa_samantha Rh1AG458700 Rh1BG415000 Rh1CG429100 Rh1DG447100
rosa_wichuraiana Rw1G041230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 298
AccII CGCG 1 cut(s) 505
AciI CCGC 7 cut(s) 5, 56, 78, 138, 170, 503, 707
AclWI GGATC 1 cut(s) 255
AcoI YGGCCR 1 cut(s) 90
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 2 cut(s) 74, 300
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 417
AjnI CCWGG 3 cut(s) 310, 493, 738
AluBI AGCT 3 cut(s) 641, 749, 757
AluI AGCT 3 cut(s) 641, 749, 757
Alw21I GWGCWC 1 cut(s) 661
AlwI GGATC 1 cut(s) 255
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 356, 602, 746
AseI ATTAAT 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 298
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 7, 464
AsuHPI GGTGA 5 cut(s) 112, 242, 250, 540, 541
AvaII GGWCC 2 cut(s) 7, 464
BanI GGYRCC 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 524, 720
Bbv12I GWGCWC 1 cut(s) 661
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 368, 589, 758
BccI CCATC 2 cut(s) 275, 464
BceAI ACGGC 2 cut(s) 163, 632
BciT130I CCWGG 3 cut(s) 312, 495, 740
BciVI GTATCC 1 cut(s) 181
BclI TGATCA 1 cut(s) 591
BfmI CTRYAG 1 cut(s) 758
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 5 cut(s) 139, 357, 603, 708, 747
BlsI GCNGC 5 cut(s) 140, 358, 604, 709, 748
Bme1390I CCNGG 3 cut(s) 312, 495, 740
Bme18I GGWCC 2 cut(s) 7, 464
BmgT120I GGNCC 2 cut(s) 7, 464
BmiI GGNNCC 2 cut(s) 300, 465
BmrFI CCNGG 3 cut(s) 312, 495, 740
BmrI ACTGGG 2 cut(s) 581, 671
BmsI GCATC 3 cut(s) 70, 325, 634
BmuI ACTGGG 2 cut(s) 581, 671
BpiI GAAGAC 2 cut(s) 524, 720
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 2 cut(s) 158, 348
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 3 cut(s) 310, 493, 739
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 4 cut(s) 10, 430, 587, 666
Bse3DI GCAATG 2 cut(s) 406, 621
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 3 cut(s) 312, 495, 740
BseDI CCNNGG 3 cut(s) 310, 493, 739
BseGI GGATG 2 cut(s) 499, 676
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 2 cut(s) 406, 621
BseMII CTCAG 2 cut(s) 172, 339
BseNI ACTGG 4 cut(s) 10, 430, 587, 666
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 3 cut(s) 368, 589, 758
BsgI GTGCAG 1 cut(s) 671
Bsh1236I CGCG 1 cut(s) 505
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 661
BsiSI CCGG 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 3 cut(s) 247, 442, 591
BspACI CCGC 7 cut(s) 5, 56, 78, 138, 170, 503, 707
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 2 cut(s) 171, 340
BspFNI CGCG 1 cut(s) 505
BspLI GGNNCC 2 cut(s) 300, 465
BspPI GGATC 1 cut(s) 255
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 406, 621
BsrI ACTGG 4 cut(s) 10, 430, 587, 666
BssECI CCNNGG 3 cut(s) 310, 493, 739
BssMI GATC 3 cut(s) 247, 442, 591
Bst2UI CCWGG 3 cut(s) 312, 495, 740
Bst4CI ACNGT 4 cut(s) 124, 193, 260, 671
Bst6I CTCTTC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 2 cut(s) 628, 705
BstDEI CTNAG 3 cut(s) 158, 348, 733
BstF5I GGATG 2 cut(s) 499, 676
BstFNI CGCG 1 cut(s) 505
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 3 cut(s) 250, 445, 594
BstMBI GATC 3 cut(s) 247, 442, 591
BstMWI GCNNNNNNNGC 3 cut(s) 344, 353, 611
BstNI CCWGG 3 cut(s) 312, 495, 740
BstNSI RCATGY 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 310, 493, 738
BstSFI CTRYAG 1 cut(s) 758
BstUI CGCG 1 cut(s) 505
BstV1I GCAGC 3 cut(s) 368, 589, 758
BstV2I GAAGAC 2 cut(s) 524, 720
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 2 cut(s) 499, 676
BtsIMutI CAGTG 3 cut(s) 17, 594, 659
Cac8I GCNNGC 2 cut(s) 628, 705
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 7, 464
Csp6I GTAC 2 cut(s) 73, 299
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviAII CATG 1 cut(s) 212
CviJI RGCY 9 cut(s) 92, 141, 178, 223, 347, 641, 710, 749, 757
CviKI_1 RGCY 9 cut(s) 92, 141, 178, 223, 347, 641, 710, 749, 757
CviQI GTAC 2 cut(s) 73, 299
DdeI CTNAG 3 cut(s) 158, 348, 733
DpnI GATC 3 cut(s) 249, 444, 593
DpnII GATC 3 cut(s) 247, 442, 591
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 7, 464
Eco57I CTGAAG 1 cut(s) 732
EcoRII CCWGG 3 cut(s) 310, 493, 738
FaeI CATG 1 cut(s) 215
FatI CATG 1 cut(s) 211
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 5 cut(s) 139, 357, 603, 708, 747
FokI GGATG 2 cut(s) 486, 663
Fsp4HI GCNGC 5 cut(s) 139, 357, 603, 708, 747
GlaI GCGC 1 cut(s) 398
GluI GCNGC 5 cut(s) 139, 357, 603, 708, 747
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 135
HhaI GCGC 1 cut(s) 399
Hin1II CATG 1 cut(s) 215
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 2 cut(s) 674, 723
HindII GTYRAC 2 cut(s) 674, 723
HinfI GANTC 3 cut(s) 304, 532, 545
HpaI GTTAAC 1 cut(s) 723
HpaII CCGG 1 cut(s) 135
HphI GGTGA 5 cut(s) 112, 242, 250, 540, 541
Hpy166II GTNNAC 4 cut(s) 75, 287, 674, 723
Hpy188I TCNGA 2 cut(s) 39, 435
Hpy188III TCNNGA 4 cut(s) 160, 467, 563, 680
Hpy8I GTNNAC 4 cut(s) 75, 287, 674, 723
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 4 cut(s) 16, 97, 238, 592
HpyCH4III ACNGT 4 cut(s) 124, 193, 260, 671
HpyCH4V TGCA 7 cut(s) 61, 338, 510, 605, 614, 652, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 353, 611
HpyF3I CTNAG 3 cut(s) 158, 348, 733
Hsp92II CATG 1 cut(s) 215
HspAI GCGC 1 cut(s) 397
KpnI GGTACC 1 cut(s) 302
Ksp22I TGATCA 1 cut(s) 591
KspAI GTTAAC 1 cut(s) 723
Kzo9I GATC 3 cut(s) 247, 442, 591
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 3 cut(s) 368, 589, 758
LweI GCATC 3 cut(s) 70, 325, 634
MaeIII GTNAC 3 cut(s) 260, 421, 529
MalI GATC 3 cut(s) 249, 444, 593
MboI GATC 3 cut(s) 247, 442, 591
MboII GAAGA 6 cut(s) 31, 185, 253, 374, 529, 725
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 1 cut(s) 164
MlyI GAGTC 2 cut(s) 526, 539
MnlI CCTC 7 cut(s) 37, 40, 102, 343, 535, 561, 570
MseI TTAA 3 cut(s) 330, 381, 722
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 312, 495, 740
MvaI CCWGG 3 cut(s) 312, 495, 740
MvnI CGCG 1 cut(s) 505
MwoI GCNNNNNNNGC 3 cut(s) 344, 353, 611
NdeII GATC 3 cut(s) 247, 442, 591
NlaIII CATG 1 cut(s) 215
NlaIV GGNNCC 2 cut(s) 300, 465
NmuCI GTSAC 1 cut(s) 529
NspI RCATGY 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 168
PfeI GAWTC 1 cut(s) 304
PkrI GCNGC 5 cut(s) 140, 358, 604, 709, 748
PleI GAGTC 2 cut(s) 526, 539
PpsI GAGTC 2 cut(s) 526, 539
PshBI ATTAAT 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 310, 493, 738
PspGI CCWGG 3 cut(s) 310, 493, 738
PspN4I GGNNCC 2 cut(s) 300, 465
PspPI GGNCC 2 cut(s) 7, 464
RsaI GTAC 2 cut(s) 74, 300
RsaNI GTAC 2 cut(s) 73, 299
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 3 cut(s) 330, 381, 722
SatI GCNGC 5 cut(s) 139, 357, 603, 708, 747
Sau3AI GATC 3 cut(s) 247, 442, 591
Sau96I GGNCC 2 cut(s) 7, 464
SchI GAGTC 2 cut(s) 526, 539
ScrFI CCNGG 3 cut(s) 312, 495, 740
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 3 cut(s) 70, 325, 634
SfcI CTRYAG 1 cut(s) 758
SinI GGWCC 2 cut(s) 7, 464
Sse9I AATT 1 cut(s) 164
SsiI CCGC 7 cut(s) 5, 56, 78, 138, 170, 503, 707
StyD4I CCNGG 3 cut(s) 310, 493, 738
TaaI ACNGT 4 cut(s) 124, 193, 260, 671
TaqI TCGA 1 cut(s) 540
TasI AATT 1 cut(s) 164
TatI WGTACW 1 cut(s) 72
TauI GCSGC 2 cut(s) 141, 710
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 3 cut(s) 330, 381, 722
Tru9I TTAA 3 cut(s) 330, 381, 722
TscAI CASTG 3 cut(s) 17, 594, 666
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 3 cut(s) 356, 602, 746
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 3 cut(s) 17, 594, 666
VpaK11BI GGWCC 2 cut(s) 7, 464
VspI ATTAAT 1 cut(s) 330
XceI RCATGY 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.