MD07G1300100.v1.1

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009638
Physical Location & Seq
Reverse (-)
35898859 .. 35899764
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1300100.v1.1.491

Sequence Viewer

Length: 906 bp
ATGTGTGGGGTCTGCCAGCAAGCCCCAGCCGTCGTCACCTGCAAGGCTGACGCCGCTGCTCTCTGCGTCAGCTGCGACACCGACATCCACTCAGCTAACCCCATCGTCCGCCGCCACGAGTGGGTCCCGGTCGAGCCTTTTTACGACGCCGCCGAGTCTATCGTCGTCAAGTCAACAACTGGCCCGTCATCCGCCGCCGCGGCTCTCAACTACCTCGTCCCAAACGGAGACGTTGTATTTAAAGACATTGAAAACAACGCCGCGTCGAGCTGGCTGATCCCCAACCCGAATTTCAACTCGAAGCTCCATATGGATATCGCTCCGGACATCCTAAAGTCCTCCGATGATCTCATCTTCCCCGAAATTGATTCACTGCTGGAGTTCGATTACCCTACCTCAGTACACACTATTTCGGGTTCGGGTGCGTGTAATGACAGCGTTGTTCCGGTTCAACCCGACCCGATTCCACCGCCATCGTTCAACATGAACTACAACATTTCGGGTCCCGCCGATCACAACTGCTTCGACCTGGACTTTTGCAGAAGCAAGCTCTATTCTTCATTCAACAACCCCCTCAATCTCCACTCCTACAAGCACTCTGGGTTGGCAAACAAGAAAACCGTGACCATTCAGGGTGTGGGCAAAGATCAATCTGTGCTGCTGGCAACAAGTAAGTCCAAGAAGCAGAACAAGCCTGCTGCTCTGCTGCATAAGTCTGTGATGAAGCAGGAGTTCCGCAGGATGGCGAAGGCGGTCGGTAATCAGGTGGCGGATAACCATTACAGGCCGGATCTGAAAAAAGCAGCCCTTGCAAGGTTGAGTGCTGTTCACAAGAGCCTCAAGATTGCCAAGTCTGGTGTCAAGAAGAGGAACAGGCAAGCTGTTAAAGTCTATGGCAGGAAATGA

Protein Analysis

302

Amino Acids

32.77

Weight (kDa)

9.08

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28e PF01778 184 - 279 9.4e-23 Ribosomal L28e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 47
AccII CGCG 2 cut(s) 200, 263
AccIII TCCGGA 1 cut(s) 322
AclWI GGATC 2 cut(s) 271, 798
AcsI RAATTY 1 cut(s) 289
AcyI GRCGYC 2 cut(s) 51, 147
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 3 cut(s) 121, 742, 813
AgsI TTSAA 5 cut(s) 251, 295, 452, 481, 565
AjnI CCWGG 1 cut(s) 528
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 6 cut(s) 72, 95, 270, 304, 550, 881
AluI AGCT 6 cut(s) 72, 95, 270, 304, 550, 881
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 2 cut(s) 271, 798
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 2 cut(s) 181, 785
ApeKI GCWGC 6 cut(s) 56, 72, 658, 698, 706, 803
ApoI RAATTY 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 124, 182, 503
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 28
AvaII GGWCC 2 cut(s) 124, 503
BauI CACGAG 1 cut(s) 116
BbvI GCAGC 6 cut(s) 43, 59, 645, 685, 693, 815
BccI CCATC 3 cut(s) 110, 481, 736
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 530
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 47
BglI GCCNNNNNGGC 1 cut(s) 200
Bme1390I CCNGG 2 cut(s) 128, 530
Bme18I GGWCC 2 cut(s) 124, 503
BmgT120I GGNCC 3 cut(s) 124, 182, 503
BmiI GGNNCC 4 cut(s) 125, 126, 504, 505
BmrFI CCNGG 2 cut(s) 128, 530
BpmI CTGGAG 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 51, 147
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 2 cut(s) 322, 445
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 742, 813
Bse1I ACTGG 1 cut(s) 184
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 4 cut(s) 84, 188, 327, 747
BseLI CCNNNNNNNGG 3 cut(s) 121, 742, 813
BseMII CTCAG 2 cut(s) 105, 411
BseNI ACTGG 1 cut(s) 184
BseXI GCAGC 6 cut(s) 43, 59, 645, 685, 693, 815
BseYI CCCAGC 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 200, 263
Bsh1285I CGRYCG 2 cut(s) 132, 756
BshFI GGCC 2 cut(s) 183, 787
BsiEI CGRYCG 2 cut(s) 132, 756
BsiSI CCGG 4 cut(s) 128, 323, 446, 788
BslFI GGGAC 3 cut(s) 110, 203, 489
BslI CCNNNNNNNGG 3 cut(s) 121, 742, 813
BsmAI GTCTC 1 cut(s) 222
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 3 cut(s) 110, 203, 489
BsnI GGCC 2 cut(s) 183, 787
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 5 cut(s) 276, 346, 511, 646, 790
BspANI GGCC 2 cut(s) 183, 787
BspCNI CTCAG 2 cut(s) 104, 410
BspEI TCCGGA 1 cut(s) 322
BspFNI CGCG 2 cut(s) 200, 263
BspLI GGNNCC 4 cut(s) 125, 126, 504, 505
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 271, 798
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 5 cut(s) 276, 346, 511, 646, 790
BssNI GRCGYC 2 cut(s) 51, 147
BssSI CACGAG 1 cut(s) 116
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 1 cut(s) 860
BstACI GRCGYC 2 cut(s) 51, 147
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 7 cut(s) 17, 21, 272, 548, 663, 696, 879
BstDEI CTNAG 2 cut(s) 91, 397
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 4 cut(s) 84, 188, 327, 747
BstFNI CGCG 2 cut(s) 200, 263
BstKTI GATC 5 cut(s) 279, 349, 514, 649, 793
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 5 cut(s) 276, 346, 511, 646, 790
BstMCI CGRYCG 2 cut(s) 132, 756
BstMWI GCNNNNNNNGC 5 cut(s) 53, 72, 200, 691, 809
BstNI CCWGG 1 cut(s) 530
BstSCI CCNGG 2 cut(s) 126, 528
BstUI CGCG 2 cut(s) 200, 263
BstV1I GCAGC 6 cut(s) 43, 59, 645, 685, 693, 815
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BsuRI GGCC 2 cut(s) 183, 787
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 4 cut(s) 84, 188, 327, 747
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 371
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 7 cut(s) 17, 21, 272, 548, 663, 696, 879
Cfr13I GGNCC 3 cut(s) 124, 182, 503
Cfr42I CCGCGG 1 cut(s) 201
CseI GACGC 4 cut(s) 55, 59, 155, 252
Csp6I GTAC 1 cut(s) 401
CviAII CATG 1 cut(s) 484
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 2 cut(s) 91, 397
DpnI GATC 5 cut(s) 278, 348, 513, 648, 792
DpnII GATC 5 cut(s) 276, 346, 511, 646, 790
DraI TTTAAA 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 860
EarI CTCTTC 1 cut(s) 860
EciI GGCGGA 3 cut(s) 98, 181, 785
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 124, 503
EcoO109I RGGNCCY 2 cut(s) 124, 503
EcoRII CCWGG 1 cut(s) 528
EcoRV GATATC 1 cut(s) 316
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 1 cut(s) 487
FaiI YATR 5 cut(s) 309, 311, 485, 711, 894
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 3 cut(s) 110, 203, 489
FatI CATG 1 cut(s) 483
FauI CCCGC 1 cut(s) 514
FauNDI CATATG 1 cut(s) 309
FokI GGATG 4 cut(s) 71, 175, 314, 754
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 398
HaeIII GGCC 2 cut(s) 183, 787
HapII CCGG 4 cut(s) 128, 323, 446, 788
HgaI GACGC 4 cut(s) 55, 59, 155, 252
Hin1I GRCGYC 2 cut(s) 51, 147
Hin1II CATG 1 cut(s) 487
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HinfI GANTC 3 cut(s) 155, 368, 463
HpaII CCGG 4 cut(s) 128, 323, 446, 788
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 3 cut(s) 174, 403, 829
Hpy188I TCNGA 2 cut(s) 343, 795
Hpy188III TCNNGA 3 cut(s) 323, 841, 862
Hpy8I GTNNAC 3 cut(s) 174, 403, 829
Hpy99I CGWCG 4 cut(s) 35, 149, 167, 268
HpyAV CCTTC 1 cut(s) 742
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 4 cut(s) 42, 540, 709, 812
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 72, 200, 691, 809
HpyF3I CTNAG 2 cut(s) 91, 397
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 2 cut(s) 51, 147
Hsp92II CATG 1 cut(s) 487
KflI GGGWCCC 2 cut(s) 124, 503
Kpn2I TCCGGA 1 cut(s) 322
KspI CCGCGG 1 cut(s) 201
Kzo9I GATC 5 cut(s) 276, 346, 511, 646, 790
LmnI GCTCC 2 cut(s) 309, 325
Lsp1109I GCAGC 6 cut(s) 43, 59, 645, 685, 693, 815
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 34, 622
MalI GATC 5 cut(s) 278, 348, 513, 648, 792
MboI GATC 5 cut(s) 276, 346, 511, 646, 790
MboII GAAGA 3 cut(s) 346, 549, 877
MflI RGATCY 1 cut(s) 790
MluCI AATT 2 cut(s) 289, 363
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 6 cut(s) 224, 349, 406, 584, 848, 861
MroI TCCGGA 1 cut(s) 322
MseI TTAA 2 cut(s) 240, 885
MspA1I CMGCKG 3 cut(s) 56, 72, 200
MspI CCGG 4 cut(s) 128, 323, 446, 788
MspR9I CCNGG 2 cut(s) 128, 530
MvaI CCWGG 1 cut(s) 530
MvnI CGCG 2 cut(s) 200, 263
MwoI GCNNNNNNNGC 5 cut(s) 53, 72, 200, 691, 809
NciI CCSGG 1 cut(s) 128
NdeI CATATG 1 cut(s) 309
NdeII GATC 5 cut(s) 276, 346, 511, 646, 790
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 4 cut(s) 125, 126, 504, 505
NmeAIII GCCGAG 1 cut(s) 178
NmuCI GTSAC 2 cut(s) 34, 622
PaqCI CACCTGC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 2 cut(s) 368, 463
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PpuMI RGGWCCY 2 cut(s) 124, 503
Psp5II RGGWCCY 2 cut(s) 124, 503
Psp6I CCWGG 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 4 cut(s) 125, 126, 504, 505
PspPI GGNCC 3 cut(s) 124, 182, 503
PspPPI RGGWCCY 2 cut(s) 124, 503
PsuI RGATCY 1 cut(s) 790
PvuII CAGCTG 1 cut(s) 72
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
SacII CCGCGG 1 cut(s) 201
SaqAI TTAA 2 cut(s) 240, 885
Sau3AI GATC 5 cut(s) 276, 346, 511, 646, 790
Sau96I GGNCC 3 cut(s) 124, 182, 503
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 2 cut(s) 128, 530
Sfr303I CCGCGG 1 cut(s) 201
SgrBI CCGCGG 1 cut(s) 201
SinI GGWCC 2 cut(s) 124, 503
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 2 cut(s) 289, 363
StyD4I CCNGG 2 cut(s) 126, 528
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 1 cut(s) 234
TaqI TCGA 5 cut(s) 132, 266, 299, 384, 525
TasI AATT 2 cut(s) 289, 363
TatI WGTACW 1 cut(s) 400
TauI GCSGC 7 cut(s) 56, 114, 152, 197, 200, 203, 263
TfiI GAWTC 2 cut(s) 368, 463
Tru1I TTAA 2 cut(s) 240, 885
Tru9I TTAA 2 cut(s) 240, 885
TscAI CASTG 1 cut(s) 378
TseFI GTSAC 2 cut(s) 34, 622
TseI GCWGC 6 cut(s) 56, 72, 658, 698, 706, 803
Tsp45I GTSAC 2 cut(s) 34, 622
TspDTI ATGAA 3 cut(s) 500, 549, 737
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 1 cut(s) 378
VpaK11BI GGWCC 2 cut(s) 124, 503
XapI RAATTY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.