Rorug05G0458600

Oxidoreductase family, C-terminal alpha/beta domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009638
Physical Location & Seq
Reverse (-)
63160007 .. 63166912
6906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0458600.1

Sequence Viewer

Length: 3693 bp
ATGATGGAGGACGACGAGTACTGGGCTTGGGATTCCTTCTATTCAGATTGGAGCACCTTCTACAGTGGGTCTCATGATTCAGCTCCTCATTGGAAGTACGATGTGTTTTTGAGTTTTAGGGGCGAAGACACGCGAACCAGTTTCACTGACCATCTTTACCAAAAATTGGTGTGGCGAGGAATCAAAACTTTCAGGGATGACGCAGAAATTGAAAGGGGGTCAACTATTTCTGCAGAGCTCTCGGCAGCCATTAAAGATTCAAGGTTCGCAGTCATTGTTCTCTCCCCAAACTACGCTTCTTCCACTTGGTGCTTGGATGAACTTGAAATGATTAATCAATCGCGTGGAATCTCCAGACGTTTGCCGGTCTTCTATCATGTTGATCCTTCTGATGTTCGAAGACAAACTGGCCCTTTCGCCCAAGCCTTCACTAAACATGAACATGACTTCAAGGCATACCCGAAGAAGGTGCGTTGGTGGAGAGAGGCTTTGACAAGTGTGAGTAATGTTGCTGGATGGACTACTTCTAAGTATAGGTATGAAACAGAGCTTATCAAAGAAATTGTAGATGTAATAGGGGAGAAAGTGTATCCTACGTTCACATTGTCAGATTCGACAGAGAACTTGTTTGGAATTGATTTTAGATTACAGTACATAGATTTGCTTTTATATACTAGCGTAACTGATGTTCGCTTTTTGGGGATATGGGGGATGGGTGGGGTGGGTAAAACAACGATTGCTAGATTAGTTTATGAGAGAATTTCCCATAAGTATGAGGTTAGCTACTTTCTTGCTAATGTCAGACAGCAATCTGCCGAACATGGTATAGTTCATCTGCAAAAACAACTTATTTCCCTTGTATTGGAGGAAAGAGTTGCACAAGTTTGTGATGCTTATGGTGGAGCTGCAATGACCAAGAATTTATTATGTAATAAAAAGGTTCTTCTTGTTCTGGATGATGTGGATCAATTGAACCAACTTGAAATGCTTGCCGGAACTAAAGACTGGTTTGGTCCGGGGAGCAGAATTATCATCACAACAAGAGATGAACGTCTGCTGATTGAACATGGTATAGAGAAACCGTACGAGCTCCAGGGTTTAAATGACAATCAAGCTCTTCAGCTCTTTTCATGGAAAGCCTTCAAAAAATATCATCCAGAAGAAGATTATTTGGAACTGTCCAAATGTTTCGTGGATTATGCGGGAGGTCTTCCATTAGCTCTTAGAACTCTTGGATCTTCTTTGTATAAAAGAGGTCGAGATGCCTGGATCAGTACATTGGATAAACTCAAGAAAGCTCCTAATAAGACAATCTTTGAGACTCTCAAGATAAGTTACGATGGACTGGATGATATGGAAAAGAGAATTTTTCTCAATGTTGCATGCTTTCATAAGGGTGGCTACAAGGAGCGAGTAATTGAAATACTGGATAGTTGTGGCTTTCCAGCTTGTATTGTGATAGATATTCTGGTTGAGAAATCTCTCTTAACTATTTTTGACGACTATGTCATGATGCATGATCTGATACAAGAAATGGGACGGGATATTGTTCGTCAAGAATCTTATGAGGAACCTGGGCAACGTAGTCGATTGTGGCTTGATAAAGACATCTTTCATGTATTTTCGAAGAATACAGGAACTGAAGCAATTGAAGGAATAGTCCTATACTTGCCTGAATTTGAACAAGCATGCTTGAATCCTGAAACTTTCTCCAAGATGTCTAGACTAAAGTTGCTCCAAATTCATAATCTGAACCTATCTCCAGGCCCAAAATATCTGTCTAATTCCTTGAGATTTCTGGAATGGAGTTTGTATCCTTCAAAATCTCTCCCACCAACTTTTCAACCAGATGGTCTTACTGAACTTAATTTGCGTCATAGCAAAGTTGATCGGCTTTGGAATGGGAAAAAGCACTTGGGTAACTTGAAGTATATTGACCTTAGTTTCTCTGAAAATTTGATCAAGACCCCAGATTTCACTGGTATTCCGAATCTTGAAAGGTTGGTTCTTGAAAGTTGCACGAATGTAGTTGAGATTCACTCATCTATTGCATTTCTCAAAAAGCTTAAAATTTTGAATTTCACAAACTGTAAAAGTATCAGAAGTCTTCCAAGTGAGATAGAAATGGAATCTCTTGAAGTTTGTATACTTTCGGGATGCTCAAAATTGAAAAGCATTCCAGAATTTTTGGGACCAATGAAAAATTTATCAATGCTTTCTTCGGATGGGACAGCTATTGAGCAATTACATCCATCATTTGAATGTTTGGTTGGCCTTATTTCACTAGATCTAAGAGATTGCAAAAGTTTATTGTGTCTTCCGAGTGTCCTCTGTCGCATGAAATCTCTTAGAAGTCTCAATATGTCTGGATGCTCACAACTGTGCAAATTCCCTGATAGTGTAGGGCAGATTGAATGCTTGAAAGAGTTAGATCTTAGTGGAACTGCATTAAGCGAGCTTCCATCCTCCATTGTTCTGATGAAAAATCTGGAAGTATTGTCTTTTCGTGGATGTAAAGGGCCACCACCTAAATCATGGCATTTGTTTCCCCCTTTTGGCTCATTTCAAAGAAATAGTCCCGAGCCTGCGGGTCTAGTGATGGCTTCTTTAAATAATTTGCATTCAGTGAGGAAACTGGATTTAAGCAACTGCAGTTTTGGTGAAGGAGCAATCCCAGATGATATTGGCTGTTTGTCCTCTTTAGAAGAATTAAATCTTGGGGGAAACAATTTTATCAGCCTTCCTGCAAGCATCAGATGGCTCTCTCAGCTTATTTTATTAAACTTGGAGGGCTGCAAAAAGCTTCAGCGGTTGCCTGACCTCCCATCAAATGAAGAATTAACTATAACAATTGACGATTGTACTTCCTTGGAAATGTTGCCTGATCCACCAAAGTTGAGCAGATTGAGGTGGTTTTCTTTTAGATGTGTCAATTGTTACAAATTGGTTGGCAGTGAAGACAGCACTAATAGACCATTTTCAATGTTAAAGCGATTCCTGCAGGGAACCCCTCCTTTTATTGTTCACAGTTTCAATATTGTTACTCCTGGAAGCGAAATTCCTGAGTGGTTCAGTAATCAAACTGTGGGAGATTCAGTTGTTGTGCAACTACCTGCTCGACCAAGTGATAACAAGTCAGTGGGGTTTGCTCTATGTGTTGTCTTTGAAGCTGAAGAAAATCCAACTGCCGTTGAACTCAATTATATACGAAGTCATGCATGTGGAATTAAATGTCTTTCCAAAGTAGAAGGATCTTTGTCAACCTCTTTACTTGTAAAAGGGCTTTTTCATCATAAAGTAGGCCAGGTTATGTCAGATCACCTTTGGTTACTCTATGCATCTTTCAAAGACTATGATCCTAGAAATTATTGGAAAGGAGTATTCTATGAGATTGAGTTTTCATTTAAAACCTTTTGTTCTATGGAAAACAACAAGTGTTTGAAGCTGAAGAAGTGTGGGGTCCGTGTGGTGGACAAGCAAGATGTGGTAGATCTTAACAAAACAATGAACCAATCCAACAGCAGCTTTTATCTCGACAAGGCTATGTATGACCTCCATTGTGATCTTGACAAGTCAGCAAGTGTTCAATGTGCTGAAGTTGGGCAAACAATTGAACATTCTGATAAGGCAGGACCAAGTGGAAGTGGTATCTCTGACATGGAATCACTCAGCAAAAGATTGAAACACGAGTAA

Protein Analysis

1230

Amino Acids

139.52

Weight (kDa)

6.05

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 32 - 211 5.7e-49 TIR domain
TIR_2 PF13676 35 - 129 4.1e-15 TIR domain
NB-ARC PF00931 228 - 383 4.1e-19 NB-ARC domain
NACHT PF05729 235 - 376 1.9e-06 NACHT domain
WHD_ROQ1 PF23282 452 - 521 1.8e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 747 - 838 2e-09 Leucine-rich repeat region
LRR_13 PF23286 797 - 964 5.6e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 867 - 976 1.2e-09 Leucine-rich repeat region
C-JID PF20160 1016 - 1162 1.3e-13 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1505
Acc36I ACCTGC 1 cut(s) 3121
AccB7I CCANNNNNTGG 1 cut(s) 166
AccI GTMKAC 1 cut(s) 2146
AccII CGCG 2 cut(s) 133, 343
AciI CCGC 3 cut(s) 1202, 2588, 2809
AclWI GGATC 7 cut(s) 377, 972, 1243, 1277, 2879, 3259, 3350
AcuI CTGAAG 6 cut(s) 1103, 1662, 2789, 3192, 3467, 3615
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 6 cut(s) 20, 98, 653, 1085, 1276, 2863
AfiI CCNNNNNNNGG 5 cut(s) 166, 466, 862, 2555, 3469
AjnI CCWGG 6 cut(s) 1092, 1265, 1573, 1762, 3046, 3303
AjuI GAANNNNNNNTTGG 8 cut(s) 280, 312, 1898, 1930, 2253, 2285, 2700, 2732
AleI CACNNNNGTG 1 cut(s) 2380
Alw21I GWGCWC 3 cut(s) 56, 240, 1092
Alw26I GTCTC 3 cut(s) 75, 1313, 2360
AlwI GGATC 7 cut(s) 377, 972, 1243, 1277, 2879, 3259, 3350
AlwNI CAGNNNCTG 1 cut(s) 1640
Ama87I CYCGRG 1 cut(s) 2579
AoxI GGCC 5 cut(s) 409, 1765, 2272, 2519, 3301
ApeKI GCWGC 4 cut(s) 245, 905, 2793, 3522
ArsI GACNNNNNNTTYG 4 cut(s) 1763, 1795, 2560, 2592
AseI ATTAAT 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 1139
AspS9I GGNCC 7 cut(s) 410, 1013, 1766, 2192, 2519, 3460, 3632
AsuC2I CCSGG 1 cut(s) 1017
AsuHPI GGTGA 2 cut(s) 2672, 3311
AsuII TTCGAA 2 cut(s) 397, 1625
AvaI CYCGRG 1 cut(s) 2579
AvaII GGWCC 4 cut(s) 1013, 2192, 3460, 3632
BanII GRGCYC 2 cut(s) 240, 1092
BarI GAAGNNNNNNTAC 2 cut(s) 2130, 2162
BauI CACGAG 1 cut(s) 3686
BbsI GAAGAC 7 cut(s) 132, 361, 406, 1202, 2099, 2309, 2964
Bbv12I GWGCWC 3 cut(s) 56, 240, 1092
BbvI GCAGC 4 cut(s) 257, 892, 2780, 3534
BceAI ACGGC 1 cut(s) 3173
BcgI CGANNNNNNTGC 6 cut(s) 222, 256, 451, 485, 1568, 1602
BciT130I CCWGG 6 cut(s) 1094, 1267, 1575, 1764, 3048, 3305
BciVI GTATCC 2 cut(s) 600, 1824
BclI TGATCA 1 cut(s) 1959
BcnI CCSGG 1 cut(s) 1017
BcoDI GTCTC 3 cut(s) 75, 1313, 2360
BfaI CTAG 6 cut(s) 675, 741, 1722, 2285, 2594, 3360
BfmI CTRYAG 4 cut(s) 61, 231, 2650, 2999
BfuAI ACCTGC 1 cut(s) 3121
BfuI GTATCC 2 cut(s) 600, 1824
BglII AGATCT 3 cut(s) 2287, 2431, 3490
BisI GCNGC 4 cut(s) 246, 906, 2794, 3523
BlsI GCNGC 4 cut(s) 247, 907, 2795, 3524
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 7 cut(s) 1017, 1094, 1267, 1575, 1764, 3048, 3305
Bme18I GGWCC 4 cut(s) 1013, 2192, 3460, 3632
BmeT110I CYCGRG 1 cut(s) 2579
BmgT120I GGNCC 7 cut(s) 410, 1013, 1766, 2192, 2519, 3460, 3632
BmiI GGNNCC 4 cut(s) 1572, 2193, 3007, 3461
BmrFI CCNGG 7 cut(s) 1017, 1094, 1267, 1575, 1764, 3048, 3305
BmrI ACTGGG 1 cut(s) 31
BmsI GCATC 7 cut(s) 880, 1252, 1503, 2147, 2360, 2760, 3347
BmuI ACTGGG 1 cut(s) 31
BpiI GAAGAC 7 cut(s) 132, 361, 406, 1202, 2099, 2309, 2964
BplI GAGNNNNNCTC 2 cut(s) 2024, 2056
BpmI CTGGAG 3 cut(s) 337, 1076, 1746
Bpu14I TTCGAA 2 cut(s) 397, 1625
BpuEI CTTGAG 3 cut(s) 1274, 1310, 1810
BpuMI CCSGG 1 cut(s) 1017
BsaBI GATNNNNATC 1 cut(s) 963
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 4 cut(s) 1016, 1093, 1574, 2868
BsaXI ACNNNNNCTCC 4 cut(s) 572, 602, 3081, 3111
Bsc4I CCNNNNNNNGG 5 cut(s) 166, 466, 862, 2555, 3469
Bse118I RCCGGY 1 cut(s) 364
Bse1I ACTGG 8 cut(s) 26, 138, 412, 1010, 1350, 1431, 1984, 2640
Bse3DI GCAATG 1 cut(s) 915
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 6 cut(s) 1094, 1267, 1575, 1764, 3048, 3305
BseDI CCNNGG 4 cut(s) 1016, 1093, 1574, 2868
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 5 cut(s) 166, 466, 862, 2555, 3469
BseMI GCAATG 1 cut(s) 915
BseMII CTCAG 3 cut(s) 2780, 3054, 3682
BseNI ACTGG 8 cut(s) 26, 138, 412, 1010, 1350, 1431, 1984, 2640
BseRI GAGGAG 1 cut(s) 75
BseXI GCAGC 4 cut(s) 257, 892, 2780, 3534
Bsh1236I CGCG 2 cut(s) 133, 343
BshFI GGCC 5 cut(s) 411, 1767, 2274, 2521, 3303
BsiHKAI GWGCWC 3 cut(s) 56, 240, 1092
BsiHKCI CYCGRG 1 cut(s) 2579
BsiSI CCGG 3 cut(s) 365, 993, 1016
BsiWI CGTACG 1 cut(s) 1083
BslFI GGGAC 4 cut(s) 1551, 2205, 2242, 2562
BslI CCNNNNNNNGG 5 cut(s) 166, 466, 862, 2555, 3469
BsmAI GTCTC 3 cut(s) 75, 1313, 2360
BsmFI GGGAC 4 cut(s) 1551, 2205, 2242, 2562
BsmI GAATGC 3 cut(s) 2175, 2420, 2620
BsnI GGCC 5 cut(s) 411, 1767, 2274, 2521, 3303
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 2579
Bsp119I TTCGAA 2 cut(s) 397, 1625
Bsp1286I GDGCHC 3 cut(s) 56, 240, 1092
BspACI CCGC 3 cut(s) 1202, 2588, 2809
BspANI GGCC 5 cut(s) 411, 1767, 2274, 2521, 3303
BspCNI CTCAG 3 cut(s) 2779, 3055, 3681
BspFNI CGCG 2 cut(s) 133, 343
BspHI TCATGA 2 cut(s) 73, 1509
BspLI GGNNCC 4 cut(s) 1572, 2193, 3007, 3461
BspMAI CTGCAG 3 cut(s) 235, 2654, 3003
BspMI ACCTGC 1 cut(s) 3121
BspPI GGATC 7 cut(s) 377, 972, 1243, 1277, 2879, 3259, 3350
BspQI GCTCTTC 1 cut(s) 1122
BspT104I TTCGAA 2 cut(s) 397, 1625
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 915
BsrFI RCCGGY 1 cut(s) 364
BsrI ACTGG 8 cut(s) 26, 138, 412, 1010, 1350, 1431, 1984, 2640
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 4 cut(s) 1016, 1093, 1574, 2868
BssNAI GTATAC 1 cut(s) 2147
BssSI CACGAG 1 cut(s) 3686
BssT1I CCWWGG 1 cut(s) 2868
Bst1107I GTATAC 1 cut(s) 2147
Bst2BI CACGAG 1 cut(s) 3686
Bst2UI CCWGG 6 cut(s) 1094, 1267, 1575, 1764, 3048, 3305
Bst4CI ACNGT 8 cut(s) 65, 651, 1083, 1179, 2090, 2382, 3029, 3085
Bst6I CTCTTC 1 cut(s) 1122
BstBI TTCGAA 2 cut(s) 397, 1625
BstC8I GCNNGC 6 cut(s) 990, 1384, 1690, 2456, 2586, 2749
BstDEI CTNAG 9 cut(s) 528, 1223, 1940, 2291, 2348, 2435, 2766, 3063, 3668
BstFNI CGCG 2 cut(s) 133, 343
BstMAI GTCTC 3 cut(s) 75, 1313, 2360
BstMWI GCNNNNNNNGC 2 cut(s) 2767, 2998
BstNI CCWGG 6 cut(s) 1094, 1267, 1575, 1764, 3048, 3305
BstNSI RCATGY 3 cut(s) 1386, 1692, 3222
BstSCI CCNGG 7 cut(s) 1015, 1092, 1265, 1573, 1762, 3046, 3303
BstSFI CTRYAG 4 cut(s) 61, 231, 2650, 2999
BstUI CGCG 2 cut(s) 133, 343
BstV1I GCAGC 4 cut(s) 257, 892, 2780, 3534
BstV2I GAAGAC 7 cut(s) 132, 361, 406, 1202, 2099, 2309, 2964
BstX2I RGATCY 5 cut(s) 1235, 2287, 2431, 3251, 3490
BstYI RGATCY 5 cut(s) 1235, 2287, 2431, 3251, 3490
BstZ17I GTATAC 1 cut(s) 2147
BsuI GTATCC 2 cut(s) 600, 1824
BsuRI GGCC 5 cut(s) 411, 1767, 2274, 2521, 3303
BtsI GCAGTG 1 cut(s) 2959
BtsIMutI CAGTG 6 cut(s) 70, 144, 1977, 2631, 2959, 3144
BveI ACCTGC 1 cut(s) 3121
Cac8I GCNNGC 6 cut(s) 990, 1384, 1690, 2456, 2586, 2749
CaiI CAGNNNCTG 1 cut(s) 1640
CciI TCATGA 2 cut(s) 73, 1509
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 7 cut(s) 410, 1013, 1766, 2192, 2519, 3460, 3632
CseI GACGC 2 cut(s) 209, 1862
Csp6I GTAC 6 cut(s) 19, 97, 652, 1084, 1275, 2862
CspCI CAANNNNNGTGG 2 cut(s) 2877, 2912
CviQI GTAC 6 cut(s) 19, 97, 652, 1084, 1275, 2862
DdeI CTNAG 9 cut(s) 528, 1223, 1940, 2291, 2348, 2435, 2766, 3063, 3668
DraI TTTAAA 3 cut(s) 1101, 2610, 3406
DraIII CACNNNGTG 1 cut(s) 309
DrdI GACNNNNNNGTC 1 cut(s) 1505
DseDI GACNNNNNNGTC 1 cut(s) 1505
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Ecl136II GAGCTC 2 cut(s) 238, 1090
Eco130I CCWWGG 1 cut(s) 2868
Eco24I GRGCYC 2 cut(s) 240, 1092
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 4 cut(s) 1013, 2192, 3460, 3632
Eco53kI GAGCTC 2 cut(s) 238, 1090
Eco57I CTGAAG 6 cut(s) 1103, 1662, 2789, 3192, 3467, 3615
Eco88I CYCGRG 1 cut(s) 2579
EcoICRI GAGCTC 2 cut(s) 238, 1090
EcoRII CCWGG 6 cut(s) 1092, 1265, 1573, 1762, 3046, 3303
EcoT14I CCWWGG 1 cut(s) 2868
EcoT22I ATGCAT 3 cut(s) 1518, 3220, 3340
EcoT38I GRGCYC 2 cut(s) 240, 1092
ErhI CCWWGG 1 cut(s) 2868
FalI AAGNNNNNCTT 2 cut(s) 1298, 1330
FaqI GGGAC 4 cut(s) 1551, 2205, 2242, 2562
FauI CCCGC 2 cut(s) 1195, 2581
FbaI TGATCA 1 cut(s) 1959
FblI GTMKAC 1 cut(s) 2146
Fnu4HI GCNGC 4 cut(s) 246, 906, 2794, 3523
FriOI GRGCYC 2 cut(s) 240, 1092
Fsp4HI GCNGC 4 cut(s) 246, 906, 2794, 3523
FspBI CTAG 6 cut(s) 675, 741, 1722, 2285, 2594, 3360
GluI GCNGC 4 cut(s) 246, 906, 2794, 3523
GsuI CTGGAG 3 cut(s) 337, 1076, 1746
HaeIII GGCC 5 cut(s) 411, 1767, 2274, 2521, 3303
HapII CCGG 3 cut(s) 365, 993, 1016
HgaI GACGC 2 cut(s) 209, 1862
HincII GTYRAC 2 cut(s) 222, 3261
HindII GTYRAC 2 cut(s) 222, 3261
HindIII AAGCTT 2 cut(s) 2063, 2801
HpaII CCGG 3 cut(s) 365, 993, 1016
HphI GGTGA 2 cut(s) 2672, 3311
Hpy166II GTNNAC 6 cut(s) 222, 600, 2147, 3025, 3261, 3472
Hpy8I GTNNAC 6 cut(s) 222, 600, 2147, 3025, 3261, 3472
Hpy99I CGWCG 1 cut(s) 17
HpyCH4III ACNGT 8 cut(s) 65, 651, 1083, 1179, 2090, 2382, 3029, 3085
HpyCH4IV ACGT 4 cut(s) 358, 596, 1051, 1582
HpyF10VI GCNNNNNNNGC 2 cut(s) 2767, 2998
HpyF3I CTNAG 9 cut(s) 528, 1223, 1940, 2291, 2348, 2435, 2766, 3063, 3668
HpySE526I ACGT 4 cut(s) 358, 596, 1051, 1582
Ksp22I TGATCA 1 cut(s) 1959
LguI GCTCTTC 1 cut(s) 1122
LmnI GCTCC 9 cut(s) 51, 88, 902, 1020, 1095, 1303, 1408, 1740, 2666
Lsp1109I GCAGC 4 cut(s) 257, 892, 2780, 3534
LweI GCATC 7 cut(s) 880, 1252, 1503, 2147, 2360, 2760, 3347
MaeI CTAG 6 cut(s) 675, 741, 1722, 2285, 2594, 3360
MaeII ACGT 4 cut(s) 358, 596, 1051, 1582
MaeIII GTNAC 6 cut(s) 679, 1334, 1919, 2936, 3040, 3327
MfeI CAATTG 5 cut(s) 968, 1647, 2850, 2932, 3609
MflI RGATCY 5 cut(s) 1235, 2287, 2431, 3251, 3490
MhlI GDGCHC 3 cut(s) 56, 240, 1092
MlyI GAGTC 1 cut(s) 1315
MmeI TCCRAC 2 cut(s) 3206, 3540
Mph1103I ATGCAT 3 cut(s) 1518, 3220, 3340
MroXI GAANNNNTTC 1 cut(s) 1139
MslI CAYNNNNRTG 6 cut(s) 441, 771, 1395, 2260, 2380, 3219
MspA1I CMGCKG 1 cut(s) 2809
MspI CCGG 3 cut(s) 365, 993, 1016
MspR9I CCNGG 7 cut(s) 1017, 1094, 1267, 1575, 1764, 3048, 3305
MunI CAATTG 5 cut(s) 968, 1647, 2850, 2932, 3609
Mva1269I GAATGC 3 cut(s) 2175, 2420, 2620
MvaI CCWGG 6 cut(s) 1094, 1267, 1575, 1764, 3048, 3305
MvnI CGCG 2 cut(s) 133, 343
MwoI GCNNNNNNNGC 2 cut(s) 2767, 2998
NciI CCSGG 1 cut(s) 1017
NlaIV GGNNCC 4 cut(s) 1572, 2193, 3007, 3461
NmeAIII GCCGAG 1 cut(s) 221
NsiI ATGCAT 3 cut(s) 1518, 3220, 3340
NspI RCATGY 3 cut(s) 1386, 1692, 3222
NspV TTCGAA 2 cut(s) 397, 1625
OliI CACNNNNGTG 1 cut(s) 2380
PaeI GCATGC 2 cut(s) 1386, 1692
PagI TCATGA 2 cut(s) 73, 1509
PciSI GCTCTTC 1 cut(s) 1122
PctI GAATGC 3 cut(s) 2175, 2420, 2620
PdmI GAANNNNTTC 1 cut(s) 1139
Pfl23II CGTACG 1 cut(s) 1083
PflFI GACNNNGTC 1 cut(s) 1505
PflMI CCANNNNNTGG 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 3046
PkrI GCNGC 4 cut(s) 247, 907, 2795, 3524
PleI GAGTC 1 cut(s) 1315
PpsI GAGTC 1 cut(s) 1315
PshBI ATTAAT 1 cut(s) 333
Psp124BI GAGCTC 2 cut(s) 240, 1092
Psp6I CCWGG 6 cut(s) 1092, 1265, 1573, 1762, 3046, 3303
PspGI CCWGG 6 cut(s) 1092, 1265, 1573, 1762, 3046, 3303
PspLI CGTACG 1 cut(s) 1083
PspN4I GGNNCC 4 cut(s) 1572, 2193, 3007, 3461
PspPI GGNCC 7 cut(s) 410, 1013, 1766, 2192, 2519, 3460, 3632
PsrI GAACNNNNNNTAC 4 cut(s) 581, 613, 672, 704
PstI CTGCAG 3 cut(s) 235, 2654, 3003
PstNI CAGNNNCTG 1 cut(s) 1640
PsuI RGATCY 5 cut(s) 1235, 2287, 2431, 3251, 3490
PsyI GACNNNGTC 1 cut(s) 1505
RsaI GTAC 6 cut(s) 20, 98, 653, 1085, 1276, 2863
RsaNI GTAC 6 cut(s) 19, 97, 652, 1084, 1275, 2862
RseI CAYNNNNRTG 6 cut(s) 441, 771, 1395, 2260, 2380, 3219
SacI GAGCTC 2 cut(s) 240, 1092
SapI GCTCTTC 1 cut(s) 1122
SatI GCNGC 4 cut(s) 246, 906, 2794, 3523
Sau96I GGNCC 7 cut(s) 410, 1013, 1766, 2192, 2519, 3460, 3632
SbfI CCTGCAGG 1 cut(s) 3003
ScaI AGTACT 1 cut(s) 20
SchI GAGTC 1 cut(s) 1315
ScrFI CCNGG 7 cut(s) 1017, 1094, 1267, 1575, 1764, 3048, 3305
SdaI CCTGCAGG 1 cut(s) 3003
SduI GDGCHC 3 cut(s) 56, 240, 1092
SfaNI GCATC 7 cut(s) 880, 1252, 1503, 2147, 2360, 2760, 3347
SfcI CTRYAG 4 cut(s) 61, 231, 2650, 2999
SfuI TTCGAA 2 cut(s) 397, 1625
SinI GGWCC 4 cut(s) 1013, 2192, 3460, 3632
SmiMI CAYNNNNRTG 6 cut(s) 441, 771, 1395, 2260, 2380, 3219
SmlI CTYRAG 3 cut(s) 1289, 1325, 1789
SmoI CTYRAG 3 cut(s) 1289, 1325, 1789
SphI GCATGC 2 cut(s) 1386, 1692
Sse8387I CCTGCAGG 1 cut(s) 3003
SsiI CCGC 3 cut(s) 1202, 2588, 2809
SspI AATATT 1 cut(s) 3037
SspMI CTAG 6 cut(s) 675, 741, 1722, 2285, 2594, 3360
SstI GAGCTC 2 cut(s) 240, 1092
StyD4I CCNGG 7 cut(s) 1015, 1092, 1265, 1573, 1762, 3046, 3303
StyI CCWWGG 1 cut(s) 2868
TaaI ACNGT 8 cut(s) 65, 651, 1083, 1179, 2090, 2382, 3029, 3085
TaiI ACGT 4 cut(s) 361, 599, 1054, 1585
TaqI TCGA 7 cut(s) 397, 614, 1258, 1588, 1625, 3118, 3534
TatI WGTACW 4 cut(s) 18, 651, 1274, 2861
TscAI CASTG 6 cut(s) 70, 151, 1984, 2631, 2959, 3144
TseI GCWGC 4 cut(s) 245, 905, 2793, 3522
TspGWI ACGGA 1 cut(s) 3452
TspRI CASTG 6 cut(s) 70, 151, 1984, 2631, 2959, 3144
Tth111I GACNNNGTC 1 cut(s) 1505
Van91I CCANNNNNTGG 1 cut(s) 166
VpaK11BI GGWCC 4 cut(s) 1013, 2192, 3460, 3632
VspI ATTAAT 1 cut(s) 333
XbaI TCTAGA 1 cut(s) 1721
XceI RCATGY 3 cut(s) 1386, 1692, 3222
XcmI CCANNNNNNNNNTGG 3 cut(s) 310, 1189, 2532
XmiI GTMKAC 1 cut(s) 2146
XmnI GAANNNNTTC 1 cut(s) 1139
XspI CTAG 6 cut(s) 675, 741, 1722, 2285, 2594, 3360
ZrmI AGTACT 1 cut(s) 20
Zsp2I ATGCAT 3 cut(s) 1518, 3220, 3340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.