MD07G1304300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011044
Physical Location & Seq
Reverse (-)
36175324 .. 36176857
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1304300.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGCGACAACGCTTCGGTGCCCGCAAGCAGCGGTACCGCTTCCGTGGAGGGCGAGAAGGCCCATAGGCGGAAGAGGCCCCCGCCACTGCCAGGTGCCGAACATCCAGGCGTTCCAGCGTAGCGACATCGGAAGCTTCGCGAAGGACGCGTGGCGGAGCGCCAACGACGGATTCGAGCGGTTCCTTTTCGAGGCGAAGAAGACCGCCGAACGCCTCGACCGTCAGTACGCCCTCTCCCGCCGCTTCGACACAATCGCTCGCTCAGCCGCCGCTCGCGCTAGAGAAATGGATAGGGAACTCGAGATTGGCACGCGCTGGCGCGCTTTTAGCATGGATTTCAGCAGAAATTGGCCTAGGTACAGGAAGCAGCTTTCTGACTCCTTGGAGACCCCGTTGGGAAGAAGCTTTGCGACCATTTTCTTCCTGTGGTTTGCATTATCCGGATGGCTTTTTCGGATATTGTTTTTCGCTACGTGGGTGCTTCCATTTGCCGCTCCTCTTCTCATAGGAGCCGCCAACAACCTTGTTATTAAGGGCGCTTGTCCGGCTTGTAAGAGGCAATTTGTTGGTTACAAGAACCAAGTAATCCGTTGTGGAAGCTGTGGAAACACCGTGTGGCAGCCAAAATCTGACTTCTCTTCAAGAGACGGTAGAGGAGGCCCTTCTTCGTCAAAGTCAGAGCCGGAGATTATTGATGTTGAATTTGAGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

239

Amino Acids

27.12

Weight (kDa)

10.19

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 34
AccB1I GGYRCC 3 cut(s) 18, 34, 94
AccBSI CCGCTC 3 cut(s) 178, 272, 494
AccII CGCG 5 cut(s) 140, 149, 276, 313, 321
AccIII TCCGGA 1 cut(s) 440
AcsI RAATTY 1 cut(s) 701
AdeI CACNNNGTG 1 cut(s) 615
AfaI GTAC 3 cut(s) 36, 227, 359
AfiI CCNNNNNNNGG 3 cut(s) 68, 91, 190
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 2 cut(s) 642, 701
AjnI CCWGG 2 cut(s) 90, 105
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 288, 320
AluBI AGCT 4 cut(s) 135, 370, 405, 600
AluI AGCT 4 cut(s) 135, 370, 405, 600
Alw26I GTCTC 2 cut(s) 380, 639
Ama87I CYCGRG 1 cut(s) 299
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 4 cut(s) 59, 76, 350, 658
ApeKI GCWGC 3 cut(s) 29, 367, 619
ApoI RAATTY 1 cut(s) 701
Asp718I GGTACC 1 cut(s) 34
AspA2I CCTAGG 1 cut(s) 353
AspLEI GCGC 6 cut(s) 161, 278, 315, 321, 323, 539
AspS9I GGNCC 3 cut(s) 60, 77, 659
AvaI CYCGRG 1 cut(s) 299
AvrII CCTAGG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 23
BanI GGYRCC 3 cut(s) 18, 34, 94
BbsI GAAGAC 1 cut(s) 206
BbvI GCAGC 3 cut(s) 41, 379, 631
BccI CCATC 1 cut(s) 438
BciT130I CCWGG 2 cut(s) 92, 107
BcoDI GTCTC 2 cut(s) 380, 639
BfaI CTAG 2 cut(s) 279, 354
BfoI RGCGCY 2 cut(s) 162, 540
BisI GCNGC 8 cut(s) 30, 241, 267, 270, 368, 492, 513, 620
BlnI CCTAGG 1 cut(s) 353
BlpI GCTNAGC 1 cut(s) 262
BlsI GCNGC 8 cut(s) 31, 242, 268, 271, 369, 493, 514, 621
Bme1390I CCNGG 2 cut(s) 92, 107
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 3 cut(s) 60, 77, 659
BmiI GGNNCC 6 cut(s) 20, 36, 79, 96, 182, 511
BmrFI CCNGG 2 cut(s) 92, 107
BpiI GAAGAC 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 44, 353, 381
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 377, 407
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 91, 190
BseAI TCCGGA 1 cut(s) 440
BseBI CCWGG 2 cut(s) 92, 107
BseDI CCNNGG 3 cut(s) 44, 353, 381
BseGI GGATG 2 cut(s) 102, 449
BseLI CCNNNNNNNGG 3 cut(s) 68, 91, 190
BseMII CTCAG 1 cut(s) 276
BsePI GCGCGC 1 cut(s) 319
BseRI GAGGAG 2 cut(s) 486, 669
BseSI GKGCMC 1 cut(s) 23
BseXI GCAGC 3 cut(s) 41, 379, 631
Bsh1236I CGCG 5 cut(s) 140, 149, 276, 313, 321
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 61, 78, 352, 660
BshNI GGYRCC 3 cut(s) 18, 34, 94
BsiEI CGRYCG 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 441, 545, 683
BslI CCNNNNNNNGG 3 cut(s) 68, 91, 190
BsmAI GTCTC 2 cut(s) 380, 639
BsmBI CGTCTC 1 cut(s) 639
BsnI GGCC 4 cut(s) 61, 78, 352, 660
Bso31I GGTCTC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 23
Bsp13I TCCGGA 1 cut(s) 440
Bsp1720I GCTNAGC 1 cut(s) 262
Bsp68I TCGCGA 1 cut(s) 140
BspANI GGCC 4 cut(s) 61, 78, 352, 660
BspCNI CTCAG 1 cut(s) 275
BspEI TCCGGA 1 cut(s) 440
BspFNI CGCG 5 cut(s) 140, 149, 276, 313, 321
BspLI GGNNCC 6 cut(s) 20, 36, 79, 96, 182, 511
BspT107I GGYRCC 3 cut(s) 18, 34, 94
BspTNI GGTCTC 1 cut(s) 380
BsrBI CCGCTC 3 cut(s) 178, 272, 494
BssECI CCNNGG 3 cut(s) 44, 353, 381
BssHII GCGCGC 1 cut(s) 319
BssT1I CCWWGG 2 cut(s) 353, 381
Bst2UI CCWGG 2 cut(s) 92, 107
Bst4CI ACNGT 3 cut(s) 221, 613, 650
Bst6I CTCTTC 3 cut(s) 67, 504, 643
BstBAI YACGTR 1 cut(s) 474
BstC8I GCNNGC 7 cut(s) 23, 27, 259, 274, 311, 317, 321
BstDEI CTNAG 1 cut(s) 262
BstDSI CCRYGG 1 cut(s) 44
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 2 cut(s) 102, 449
BstFNI CGCG 5 cut(s) 140, 149, 276, 313, 321
BstH2I RGCGCY 2 cut(s) 162, 540
BstHHI GCGC 6 cut(s) 161, 278, 315, 321, 323, 539
BstMAI GTCTC 2 cut(s) 380, 639
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 6 cut(s) 75, 146, 263, 275, 327, 545
BstNI CCWGG 2 cut(s) 92, 107
BstSCI CCNGG 2 cut(s) 90, 105
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 5 cut(s) 140, 149, 276, 313, 321
BstV1I GCAGC 3 cut(s) 41, 379, 631
BstV2I GAAGAC 1 cut(s) 206
BsuRI GGCC 4 cut(s) 61, 78, 352, 660
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 2 cut(s) 102, 449
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 85
BtuMI TCGCGA 1 cut(s) 140
Cac8I GCNNGC 7 cut(s) 23, 27, 259, 274, 311, 317, 321
CfoI GCGC 6 cut(s) 161, 278, 315, 321, 323, 539
Cfr13I GGNCC 3 cut(s) 60, 77, 659
CseI GACGC 1 cut(s) 155
Csp6I GTAC 3 cut(s) 35, 226, 358
CviAII CATG 1 cut(s) 331
CviQI GTAC 3 cut(s) 35, 226, 358
DdeI CTNAG 1 cut(s) 262
DraIII CACNNNGTG 1 cut(s) 615
Eam1104I CTCTTC 3 cut(s) 67, 504, 643
EarI CTCTTC 3 cut(s) 67, 504, 643
EciI GGCGGA 2 cut(s) 84, 169
Eco130I CCWWGG 2 cut(s) 353, 381
Eco31I GGTCTC 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 2 cut(s) 77, 659
EcoRII CCWGG 2 cut(s) 90, 105
EcoT14I CCWWGG 2 cut(s) 353, 381
ErhI CCWWGG 2 cut(s) 353, 381
Esp3I CGTCTC 1 cut(s) 639
FaeI CATG 1 cut(s) 334
FaiI YATR 3 cut(s) 65, 332, 506
FatI CATG 1 cut(s) 330
FauI CCCGC 3 cut(s) 30, 89, 245
Fnu4HI GCNGC 8 cut(s) 30, 241, 267, 270, 368, 492, 513, 620
FokI GGATG 2 cut(s) 89, 456
Fsp4HI GCNGC 8 cut(s) 30, 241, 267, 270, 368, 492, 513, 620
FspBI CTAG 2 cut(s) 279, 354
GlaI GCGC 6 cut(s) 160, 277, 314, 320, 322, 538
GluI GCNGC 8 cut(s) 30, 241, 267, 270, 368, 492, 513, 620
HaeII RGCGCY 2 cut(s) 162, 540
HaeIII GGCC 4 cut(s) 61, 78, 352, 660
HapII CCGG 3 cut(s) 441, 545, 683
HgaI GACGC 1 cut(s) 155
HhaI GCGC 6 cut(s) 161, 278, 315, 321, 323, 539
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 6 cut(s) 159, 276, 313, 319, 321, 537
HinP1I GCGC 6 cut(s) 159, 276, 313, 319, 321, 537
HindIII AAGCTT 2 cut(s) 133, 403
HinfI GANTC 2 cut(s) 171, 377
HpaII CCGG 3 cut(s) 441, 545, 683
Hpy188I TCNGA 5 cut(s) 131, 376, 456, 631, 679
Hpy188III TCNNGA 4 cut(s) 139, 301, 441, 642
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 3 cut(s) 51, 136, 672
HpyCH4III ACNGT 3 cut(s) 221, 613, 650
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 146, 263, 275, 327, 545
HpyF3I CTNAG 1 cut(s) 262
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 6 cut(s) 159, 276, 313, 319, 321, 537
Kpn2I TCCGGA 1 cut(s) 440
KpnI GGTACC 1 cut(s) 38
LmnI GCTCC 3 cut(s) 156, 499, 509
Lsp1109I GCAGC 3 cut(s) 41, 379, 631
MaeI CTAG 2 cut(s) 279, 354
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 1 cut(s) 569
MbiI CCGCTC 3 cut(s) 178, 272, 494
MboII GAAGA 8 cut(s) 84, 208, 211, 411, 412, 491, 630, 657
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 3 cut(s) 346, 560, 701
MluI ACGCGT 1 cut(s) 147
MlyI GAGTC 1 cut(s) 371
MroI TCCGGA 1 cut(s) 440
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 441, 545, 683
MspR9I CCNGG 2 cut(s) 92, 107
MvaI CCWGG 2 cut(s) 92, 107
MvnI CGCG 5 cut(s) 140, 149, 276, 313, 321
MwoI GCNNNNNNNGC 6 cut(s) 75, 146, 263, 275, 327, 545
NlaIII CATG 1 cut(s) 334
NlaIV GGNNCC 6 cut(s) 20, 36, 79, 96, 182, 511
NruI TCGCGA 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 299
PauI GCGCGC 1 cut(s) 319
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 217
PfeI GAWTC 1 cut(s) 171
PkrI GCNGC 8 cut(s) 31, 242, 268, 271, 369, 493, 514, 621
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Ppu21I YACGTR 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 90, 105
PspGI CCWGG 2 cut(s) 90, 105
PspN4I GGNNCC 6 cut(s) 20, 36, 79, 96, 182, 511
PspPI GGNCC 3 cut(s) 60, 77, 659
PteI GCGCGC 1 cut(s) 319
RruI TCGCGA 1 cut(s) 140
RsaI GTAC 3 cut(s) 36, 227, 359
RsaNI GTAC 3 cut(s) 35, 226, 358
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 8 cut(s) 30, 241, 267, 270, 368, 492, 513, 620
Sau96I GGNCC 3 cut(s) 60, 77, 659
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 92, 107
SduI GDGCHC 1 cut(s) 23
SetI ASST 8 cut(s) 96, 137, 359, 372, 407, 476, 525, 602
Sfr274I CTCGAG 1 cut(s) 299
SlaI CTCGAG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 3 cut(s) 346, 560, 701
SspMI CTAG 2 cut(s) 279, 354
StyD4I CCNGG 2 cut(s) 90, 105
StyI CCWWGG 2 cut(s) 353, 381
TaaI ACNGT 3 cut(s) 221, 613, 650
TaiI ACGT 1 cut(s) 476
TaqI TCGA 5 cut(s) 174, 189, 216, 246, 300
TasI AATT 3 cut(s) 346, 560, 701
TauI GCSGC 5 cut(s) 243, 269, 272, 494, 515
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 92
TseI GCWGC 3 cut(s) 29, 367, 619
TspGWI ACGGA 3 cut(s) 33, 183, 578
TspRI CASTG 1 cut(s) 92
XagI CCTNNNNNAGG 1 cut(s) 188
XapI RAATTY 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 299
XmaJI CCTAGG 1 cut(s) 353
XspI CTAG 2 cut(s) 279, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.