Rroxscaffold_4G00277330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011044
Physical Location & Seq
Forward (+)
790378 .. 791943
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277330.1

Sequence Viewer

Length: 780 bp
ATGGCGACCACCAATCTCCGATGCCCTCAACTAGCGGCGGCGCCACTTCCGCTGGGCCGGGCCAGGAGGGCCAGCAGCGGCGGAAGAGCCCGAATCCCAATCGGGGCAGCCCGCCAGGTGTCGAAAATCCAGGCCTTTCAGCGCAGCGACATCGACGGCTTCGCGAAGCGGATAGCCTCCGGCGAGGCCTGGCAGGACGCGTGGCGGTCCGCCAACGACGGATTTGAACGGTTCTTGTTCGAGGCCAAGAAAACCGCCGAGCGGTTGGACCGGCAGTACTCCGTTTCGCGGCGGCTCGATTCGCTCGCTCAGTCCGCCATGATTCGCGCGCGCGAGATTGATAGGGACTTCGAGATTGGGACCCGCTGGCGCGCTTTTTCTATGGATTTCGCCAGAAATCTGCCTAGGTACAGGAAGCAGCTTAGTGATTTCTTGGACACCCCAGTTGGGAGAAGCTTTGTGACGATTTTCTTCCTGTGGCTTGCATTGTCTGGGTGGCTTTTTCGGGGTTTGATATTGGCTACATGGGTGCTGCCATTTGCTGCTCCTCTAGTCATTGGAGCTGTTGCCAATAACCTTGTTATTAAGGGGGCTTGTCCTGCTTGCAAGAGGCAATTTGTTGGTTACAAGAACCAAATAATTCGATGTCAAAGCTGTGGAAACACTGTGTGGCAGCCAAAGAAGGGAGGAGGACCGGACTTCTTTAACAGAGATAGGAGAGGCTCTTCTTCGTCAAAGAAGGAGCCGGAGATTATTGATGTTGAGTTTGAGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.21

Weight (kDa)

10.4

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccBSI CCGCTC 1 cut(s) 262
AccII CGCG 8 cut(s) 164, 200, 289, 327, 329, 331, 333, 372
AcyI GRCGYC 1 cut(s) 41
AdeI CACNNNGTG 1 cut(s) 669
AfaI GTAC 2 cut(s) 278, 410
AfiI CCNNNNNNNGG 5 cut(s) 103, 261, 288, 447, 683
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 1 cut(s) 227
AjnI CCWGG 4 cut(s) 62, 114, 129, 188
AluBI AGCT 4 cut(s) 421, 456, 563, 654
AluI AGCT 4 cut(s) 421, 456, 563, 654
AoxI GGCC 6 cut(s) 55, 60, 69, 132, 186, 243
ApeKI GCWGC 7 cut(s) 75, 107, 144, 418, 532, 542, 673
AspA2I CCTAGG 1 cut(s) 404
AspLEI GCGC 7 cut(s) 43, 144, 329, 331, 333, 372, 374
AspS9I GGNCC 7 cut(s) 55, 60, 69, 207, 268, 360, 692
AsuC2I CCSGG 1 cut(s) 59
AvaII GGWCC 4 cut(s) 207, 268, 360, 692
AvrII CCTAGG 1 cut(s) 404
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 91
BbvI GCAGC 7 cut(s) 87, 119, 156, 430, 519, 529, 685
BceAI ACGGC 1 cut(s) 172
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 4 cut(s) 64, 116, 131, 190
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 3 cut(s) 32, 405, 551
BfoI RGCGCY 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 68
BlnI CCTAGG 1 cut(s) 404
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 5 cut(s) 59, 64, 116, 131, 190
Bme18I GGWCC 4 cut(s) 207, 268, 360, 692
BmgT120I GGNCC 7 cut(s) 55, 60, 69, 207, 268, 360, 692
BmiI GGNNCC 4 cut(s) 42, 361, 362, 744
BmrFI CCNGG 5 cut(s) 59, 64, 116, 131, 190
BmrI ACTGGG 1 cut(s) 437
BmsI GCATC 1 cut(s) 11
BmuI ACTGGG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 404
BsaWI WCCGGW 1 cut(s) 694
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 261, 288, 447, 683
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 443
BseBI CCWGG 4 cut(s) 64, 116, 131, 190
BseDI CCNNGG 1 cut(s) 404
BseLI CCNNNNNNNGG 5 cut(s) 103, 261, 288, 447, 683
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 443
BsePI GCGCGC 3 cut(s) 327, 329, 370
BseRI GAGGAG 2 cut(s) 537, 702
BseXI GCAGC 7 cut(s) 87, 119, 156, 430, 519, 529, 685
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 8 cut(s) 164, 200, 289, 327, 329, 331, 333, 372
BshFI GGCC 6 cut(s) 57, 62, 71, 134, 188, 245
BshNI GGYRCC 1 cut(s) 40
BsiSI CCGG 5 cut(s) 58, 180, 271, 695, 746
BslFI GGGAC 2 cut(s) 359, 373
BslI CCNNNNNNNGG 5 cut(s) 103, 261, 288, 447, 683
BsmFI GGGAC 2 cut(s) 359, 373
BsnI GGCC 6 cut(s) 57, 62, 71, 134, 188, 245
Bsp1286I GDGCHC 1 cut(s) 91
Bsp68I TCGCGA 1 cut(s) 164
BspANI GGCC 6 cut(s) 57, 62, 71, 134, 188, 245
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 8 cut(s) 164, 200, 289, 327, 329, 331, 333, 372
BspLI GGNNCC 4 cut(s) 42, 361, 362, 744
BspQI GCTCTTC 2 cut(s) 79, 730
BspT107I GGYRCC 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 270
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 1 cut(s) 404
BssHII GCGCGC 3 cut(s) 327, 329, 370
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 4 cut(s) 64, 116, 131, 190
Bst4CI ACNGT 2 cut(s) 231, 667
Bst6I CTCTTC 2 cut(s) 79, 730
BstACI GRCGYC 1 cut(s) 41
BstC8I GCNNGC 9 cut(s) 73, 112, 306, 329, 331, 368, 372, 483, 604
BstDEI CTNAG 2 cut(s) 309, 422
BstFNI CGCG 8 cut(s) 164, 200, 289, 327, 329, 331, 333, 372
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 7 cut(s) 43, 144, 329, 331, 333, 372, 374
BstMWI GCNNNNNNNGC 5 cut(s) 49, 68, 301, 314, 599
BstNI CCWGG 4 cut(s) 64, 116, 131, 190
BstSCI CCNGG 5 cut(s) 57, 62, 114, 129, 188
BstUI CGCG 8 cut(s) 164, 200, 289, 327, 329, 331, 333, 372
BstV1I GCAGC 7 cut(s) 87, 119, 156, 430, 519, 529, 685
BsuRI GGCC 6 cut(s) 57, 62, 71, 134, 188, 245
BtsIMutI CAGTG 1 cut(s) 663
BtuMI TCGCGA 1 cut(s) 164
Cac8I GCNNGC 9 cut(s) 73, 112, 306, 329, 331, 368, 372, 483, 604
CfoI GCGC 7 cut(s) 43, 144, 329, 331, 333, 372, 374
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 7 cut(s) 55, 60, 69, 207, 268, 360, 692
CpoI CGGWCCG 1 cut(s) 207
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 277, 409
CspI CGGWCCG 1 cut(s) 207
CviAII CATG 2 cut(s) 319, 525
CviQI GTAC 2 cut(s) 277, 409
DdeI CTNAG 2 cut(s) 309, 422
DinI GGCGCC 1 cut(s) 42
DraIII CACNNNGTG 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 79, 730
EarI CTCTTC 2 cut(s) 79, 730
EciI GGCGGA 3 cut(s) 96, 199, 304
Eco130I CCWWGG 1 cut(s) 404
Eco147I AGGCCT 2 cut(s) 134, 188
Eco24I GRGCYC 1 cut(s) 91
Eco47I GGWCC 4 cut(s) 207, 268, 360, 692
EcoO109I RGGNCCY 1 cut(s) 360
EcoRII CCWGG 4 cut(s) 62, 114, 129, 188
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 91
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 322, 528
FaiI YATR 3 cut(s) 320, 383, 526
FaqI GGGAC 2 cut(s) 359, 373
FatI CATG 2 cut(s) 318, 524
FauI CCCGC 2 cut(s) 119, 371
FriOI GRGCYC 1 cut(s) 91
FspBI CTAG 3 cut(s) 32, 405, 551
GlaI GCGC 7 cut(s) 42, 143, 328, 330, 332, 371, 373
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 6 cut(s) 57, 62, 71, 134, 188, 245
HapII CCGG 5 cut(s) 58, 180, 271, 695, 746
HgaI GACGC 1 cut(s) 206
HhaI GCGC 7 cut(s) 43, 144, 329, 331, 333, 372, 374
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 2 cut(s) 322, 528
Hin6I GCGC 7 cut(s) 41, 142, 327, 329, 331, 370, 372
HinP1I GCGC 7 cut(s) 41, 142, 327, 329, 331, 370, 372
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 3 cut(s) 93, 299, 322
HpaII CCGG 5 cut(s) 58, 180, 271, 695, 746
Hpy188I TCNGA 1 cut(s) 20
Hpy188III TCNNGA 2 cut(s) 163, 352
Hpy99I CGWCG 2 cut(s) 158, 221
HpyAV CCTTC 2 cut(s) 676, 733
HpyCH4III ACNGT 2 cut(s) 231, 667
HpyCH4V TGCA 2 cut(s) 485, 606
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 68, 301, 314, 599
HpyF3I CTNAG 2 cut(s) 309, 422
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 2 cut(s) 322, 528
HspAI GCGC 7 cut(s) 41, 142, 327, 329, 331, 370, 372
KasI GGCGCC 1 cut(s) 40
KflI GGGWCCC 1 cut(s) 360
LguI GCTCTTC 2 cut(s) 79, 730
LmnI GCTCC 3 cut(s) 550, 560, 742
Lsp1109I GCAGC 7 cut(s) 87, 119, 156, 430, 519, 529, 685
LweI GCATC 1 cut(s) 11
MaeI CTAG 3 cut(s) 32, 405, 551
MaeIII GTNAC 2 cut(s) 460, 623
MauBI CGCGCGCG 2 cut(s) 327, 329
MbiI CCGCTC 1 cut(s) 262
MboII GAAGA 4 cut(s) 96, 463, 717, 720
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 2 cut(s) 614, 639
MluI ACGCGT 1 cut(s) 198
Mly113I GGCGCC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 246
MseI TTAA 2 cut(s) 585, 705
MspA1I CMGCKG 3 cut(s) 52, 78, 366
MspI CCGG 5 cut(s) 58, 180, 271, 695, 746
MspR9I CCNGG 5 cut(s) 59, 64, 116, 131, 190
MvaI CCWGG 4 cut(s) 64, 116, 131, 190
MvnI CGCG 8 cut(s) 164, 200, 289, 327, 329, 331, 333, 372
MwoI GCNNNNNNNGC 5 cut(s) 49, 68, 301, 314, 599
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 1 cut(s) 59
NlaIII CATG 2 cut(s) 322, 528
NlaIV GGNNCC 4 cut(s) 42, 361, 362, 744
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 460
NruI TCGCGA 1 cut(s) 164
PauI GCGCGC 3 cut(s) 327, 329, 370
PceI AGGCCT 2 cut(s) 134, 188
PciSI GCTCTTC 2 cut(s) 79, 730
PfeI GAWTC 3 cut(s) 93, 299, 322
PluTI GGCGCC 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 62, 114, 129, 188
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 4 cut(s) 62, 114, 129, 188
PspN4I GGNNCC 4 cut(s) 42, 361, 362, 744
PspPI GGNCC 7 cut(s) 55, 60, 69, 207, 268, 360, 692
PspPPI RGGWCCY 1 cut(s) 360
PteI GCGCGC 3 cut(s) 327, 329, 370
RruI TCGCGA 1 cut(s) 164
RsaI GTAC 2 cut(s) 278, 410
RsaNI GTAC 2 cut(s) 277, 409
Rsr2I CGGWCCG 1 cut(s) 207
RsrII CGGWCCG 1 cut(s) 207
SapI GCTCTTC 2 cut(s) 79, 730
SaqAI TTAA 2 cut(s) 585, 705
Sau96I GGNCC 7 cut(s) 55, 60, 69, 207, 268, 360, 692
ScaI AGTACT 1 cut(s) 278
ScrFI CCNGG 5 cut(s) 59, 64, 116, 131, 190
SduI GDGCHC 1 cut(s) 91
SetI ASST 7 cut(s) 120, 410, 423, 458, 565, 579, 656
SfaNI GCATC 1 cut(s) 11
SfiI GGCCNNNNNGGCC 1 cut(s) 68
SfoI GGCGCC 1 cut(s) 42
SinI GGWCC 4 cut(s) 207, 268, 360, 692
Sse9I AATT 2 cut(s) 614, 639
SseBI AGGCCT 2 cut(s) 134, 188
SspDI GGCGCC 1 cut(s) 40
SspMI CTAG 3 cut(s) 32, 405, 551
StuI AGGCCT 2 cut(s) 134, 188
StyD4I CCNGG 5 cut(s) 57, 62, 114, 129, 188
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 231, 667
TaqI TCGA 6 cut(s) 122, 153, 240, 297, 351, 643
TasI AATT 2 cut(s) 614, 639
TatI WGTACW 1 cut(s) 276
TauI GCSGC 5 cut(s) 38, 41, 81, 292, 295
TfiI GAWTC 3 cut(s) 93, 299, 322
Tru1I TTAA 2 cut(s) 585, 705
Tru9I TTAA 2 cut(s) 585, 705
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 7 cut(s) 75, 107, 144, 418, 532, 542, 673
Tsp45I GTSAC 1 cut(s) 460
TspGWI ACGGA 2 cut(s) 234, 271
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 4 cut(s) 207, 268, 360, 692
XmaJI CCTAGG 1 cut(s) 404
XspI CTAG 3 cut(s) 32, 405, 551
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.