Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010595

Physical Location & Seq

Genomic Coordinates Reverse (-)

36301302 .. 36307143

Gene Structure 5842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1306500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGACATGCCAAAGCTCCACCACCACCATGTTAGCCCACAAATTCCACCGCCTCCACCTCCGCAATTCCCCCCAGTTCCGATGCGTTCTGGACCAAATCGCCCCCAATCTGGCCGTCTCGGCGTCCCTCAGCTCCGTCTTCACCTCTGCCAACGTCATCGCCGCTGCCTCCGGCTCCAGCTCTCTCCACGGTGCCGTCACCTCCACCATCACTCAAGTCGCGGTCACCGCCGTCGCCATCGCCTCCGGGGCTTGTCTTTCCACCAAGGTCGATTTCTTGTGGCCCAAAATGGAAGCCCAGCCAGGTTCTGATGTGGTGGAGGGAGTGGACGTGACTGGATATCCTATCTTCAATGATCCAAAGGTGCAAAAGGCTGTTGCATTTGCTAAGAAAGCTCACCACGGCCAACTGCGCAAAACCGGAGACCCTTATTTAGTCCATTGCATTCATACAGGAAGAATCTTGGCCATGCTGGTTCCTGCAAGCGGCGAGCGAGCTGTTGAGACTGTTGTAGCCGGGATTCTCCATGATGTGGTGGACGATACATGTGTAAGTTTCTCCGATATACAACAAGAGTTTGGTGATGATGTCGCGAAGCTGGTAGCTGGTGTTTCCAGGTTAAGCTACATAAATCAGCTTTTACGGAGACACCGCAGGATAAATGTGAACCAGGGTAAACTTGGTCATGAGGAGGCAAATAATTTACGAGTTATGCTTCTGGGCATGGTTGATGATCCACGTGTTGTGCTTATAAAGCTTGCGGATCGTCTTCACAATATGAGAACAATTTATGCTCTCCCGCTGCCAAAGGCTCAAGCTTTTGCAAGGGAGACCTTGGTAATTTGGTGCTCGCTGGCTTCCAGATTGGGTTTGTGGGCAATGAAAGCTGAACTGGAAGATCTGTGCTTTGCTGTTCTTCAGCCTGAGATGTTCAAGAAAATGAGAGCTGATCTAGCTTCTATGTGGAGCTCTAGTAACAAAGTAGGAAATTCAAAAAGAAAATCCAGTTTGATGTCCTTAAATGGAAGAAGCTCAATTTCCGACAACGAAGGGCCCGTAGCAGTTGATGAAGATGTCACAACCATGAAGGATCTTTTGGAAGCTGTAGTGCCATTTGATGTATTGTTAGATCGAAGAAAACGGTCTAAATTTCTTAATACTCTAGGACAAGGTCTGGAGACTCACAGAAGACCAAAGGTTGTGCAAGATGCTGGAATTGCTTTGGCATCTATGGTAATTTGTGAGGAGGCCCTCGAGCAGGAGTTGATCATATCAACTTCTTATGTTCCAGGGATGGAAGTAACTTTATCGAGCCGCTTGAAAAGTTTGTATAGCATCTACAGCAAGATGAAACGGAAAGATGTCAGCATTAGTAAAGTTTATGATGCCCGTGCATTAAGGGTAGTCGTTGGAGACAATAACGGAACTTTGCATGGGCCTGCTGTCCAGTGTTGTTACAGCCTTCTTGACATTGTACACAAGCATTGGACCCCAATTGATGGTGAGTTTGATGATTACATTGTCAATCCAAAGCCTAGTGGCTATCAGTCTCTGCACACTGCAGTACAAGGTCCTGACAGATCACCTTTGGAAGTTCAAATACGAACACAGAGGATGCACGAGTACGCTGAACATGGACTCGCAGCACATTGGCTTTATAAAGAGACTGGAAATACGTTTTCTAACATAAGTAGCACGGATGAATCTGAAATAGACGTATCATCCTTTTTACCCAAAGATATTGAGATTGAGGACCAAAGTTCTACAGAAGATGATTTTTCTCAGAAGTATGATTTGCTGAAAATTGGACATCCAGTCCTTAGAGTGGAAGGGAGTCATCTTCTTGCTGCTGTTATCATTAGAGTAGAGAAAGATGGAAGGGAGTTGATAGTTGCAGTGAGCTTTGGACTAGCTGCTTCTGAAGCAGTAGCTGATAGAAAATCTTCTTTCCAAATAAAACGGTGGGAAGCCTATGCAAGATTATACAAGAAGGTGACGGATGAATGGTGGTGTGAACCAGGACATGGGGATTGGTGCAACTGCCTAGAGAGATATACACTCTGTCGAGATGGGATGTACCACAAGCAAGACCAGTTTGGGCGATTACTTCCAACCTTCATTCAGGTCATTGATTTGACAGACCAAGAAGAATCGGAATATTGGTCTGTTGTTTCTGCTGTTTTTGACGGAAGAGAGCTCGACGATGTTACATCCGCAGCAAGCTTTAGCTCAGTTCCGTCAACTTCCATGGAAACTAGCATCAACAACAAGGTGCGTTTGTTGAGGACCATGCTGCGATGGGAAGAACAGCTACGCTCAGAAGCAAGTCTTGGACAAGCAAAGCATAGCAGCAAGTTTTACCGCAGTCCAGGTTCTGTTGTCCTTGGAGAAGTGGTGATTATATGGTGGCCCGATGGTGAAATAATGCGGTTGAGAACCGGCAGCACCGCAGCAGATGCCGCAAGAAGAGTAGGACTGGAGGGAAAACTGGTCCTGGTTAACGGTCAGCTGGTTTTACCCAATACAAAGCTCACAGATGGCGACGTCGTTGAAGTCAGAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009259 GO:0009605 GO:0009987 GO:0009991 GO:0015969 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0033865 GO:0033875 GO:0034032 GO:0034035 GO:0034641 GO:0042278 GO:0042578 GO:0042594 GO:0044237 GO:0044238 GO:0044281 GO:0044464 GO:0046128 GO:0046483 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:1901068 GO:1901135 GO:1901360 GO:1901564 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00230 map00230

Pfam Domains

Protein Families

HD_4 RelA_SpoT TGS

Protein Analysis

855

Amino Acids

94.68

Weight (kDa)

6.36

Isoelectric Point (pI)

39.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HD_4	PF13328	125 - 292	2.9e-43	HD domain
HD	PF01966	145 - 260	2.8e-08	HD domain
RelA_SpoT	PF04607	438 - 557	6.9e-36	Region found in RelA / SpoT proteins
DUF7589	PF24500	595 - 730	4.4e-75	Domain of unknown function (DUF7589)
TGS	PF02824	801 - 852	1.6e-07	TGS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010595)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33820 FvH4_7g33820
malus_domestica	MD07G1306500.v1.1
prunus_persica	Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1
pyrus_communis	pycom07g27950
rosa_chinensis	RchiOBHm_Chr1g0383931
rosa_laevigata	RLG00000026085
rosa_multiflora	Rmu_sc0005139.1_g000026
rosa_roxburghii	Rroxscaffold_4G00277130
rosa_rugosa	Rorug01G0443100 Rorug01G0443200 Rorug01G0443400.1 Rorug01G0443500.1
rosa_samantha	Rh1AG469100 Rh1CG439600 Rh1DG459600
rosa_wichuraiana	Rw1G040330

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	752, 1659
AatII	GACGTC	1 cut(s)	2547
Acc16I	TGCGCA	1 cut(s)	413
AccB1I	GGYRCC	1 cut(s)	191
AccB7I	CCANNNNNTGG	3 cut(s)	532, 1499, 2024
AccII	CGCG	2 cut(s)	221, 593
AclWI	GGATC	4 cut(s)	350, 728, 771, 1098
AcoI	YGGCCR	3 cut(s)	111, 403, 465
AcsI	RAATTY	3 cut(s)	41, 988, 1148
AcuI	CTGAAG	2 cut(s)	902, 1941
AcvI	CACGTG	1 cut(s)	740
AcyI	GRCGYC	2 cut(s)	122, 2544
AfaI	GTAC	4 cut(s)	1476, 1566, 1625, 2078
AfiI	CCNNNNNNNGG	7 cut(s)	109, 485, 532, 1258, 1499, 1825, 2024
AflIII	ACRYGT	2 cut(s)	545, 739
AgsI	TTSAA	6 cut(s)	352, 934, 993, 1321, 1598, 2552
AhdI	GACNNNNNGTC	1 cut(s)	1814
AjiI	CACGTC	1 cut(s)	331
AjnI	CCWGG	7 cut(s)	301, 614, 669, 1288, 2017, 2368, 2493
AjuI	GAANNNNNNNTTGG	6 cut(s)	257, 289, 1079, 1111, 2313, 2345
Alw21I	GWGCWC	3 cut(s)	851, 971, 2199
Alw26I	GTCTC	9 cut(s)	121, 417, 497, 640, 824, 1172, 1407, 1554, 1658
AlwI	GGATC	4 cut(s)	350, 728, 771, 1098
AlwNI	CAGNNNCTG	4 cut(s)	308, 1552, 1931, 2375
Ama87I	CYCGRG	1 cut(s)	1253
AoxI	GGCC	8 cut(s)	111, 281, 403, 465, 1052, 1248, 1436, 2408
ApaI	GGGCCC	1 cut(s)	1056
ApoI	RAATTY	3 cut(s)	41, 988, 1148
Asp700I	GAANNNNTTC	1 cut(s)	1942
AspLEI	GCGC	1 cut(s)	414
AsuC2I	CCSGG	2 cut(s)	247, 517
AvaI	CYCGRG	1 cut(s)	1253
AvaII	GGWCC	6 cut(s)	91, 1488, 1571, 1753, 2286, 2491
BaeGI	GKGCMC	1 cut(s)	1056
BaeI	ACNNNNGTAYC	2 cut(s)	534, 567
BalI	TGGCCA	1 cut(s)	467
BanI	GGYRCC	1 cut(s)	191
BanII	GRGCYC	3 cut(s)	971, 1056, 2199
BarI	GAAGNNNNNNTAC	4 cut(s)	1584, 1616, 2295, 2327
BauI	CACGAG	1 cut(s)	1619
BbrPI	CACGTG	1 cut(s)	740
BbsI	GAAGAC	3 cut(s)	130, 761, 1195
Bbv12I	GWGCWC	3 cut(s)	851, 971, 2199
BbvCI	CCTCAGC	1 cut(s)	128
BccI	CCATC	9 cut(s)	215, 245, 1288, 1493, 1868, 2063, 2292, 2408, 2531
BceAI	ACGGC	4 cut(s)	98, 179, 215, 418
BciT130I	CCWGG	7 cut(s)	303, 616, 671, 1290, 2019, 2370, 2495
BclI	TGATCA	1 cut(s)	1266
BcnI	CCSGG	2 cut(s)	247, 517
BcoDI	GTCTC	9 cut(s)	121, 417, 497, 640, 824, 1172, 1407, 1554, 1658
BfaI	CTAG	7 cut(s)	953, 972, 1163, 1536, 1910, 2045, 2256
BfmI	CTRYAG	4 cut(s)	1104, 1339, 1560, 1764
BglI	GCCNNNNNGGC	2 cut(s)	119, 248
BglII	AGATCT	1 cut(s)	898
Bme1390I	CCNGG	9 cut(s)	247, 303, 517, 616, 671, 1290, 2019, 2370, 2495
Bme18I	GGWCC	6 cut(s)	91, 1488, 1571, 1753, 2286, 2491
BmeRI	GACNNNNNGTC	1 cut(s)	1814
BmeT110I	CYCGRG	1 cut(s)	1253
BmgBI	CACGTC	1 cut(s)	331
BmiI	GGNNCC	5 cut(s)	175, 193, 477, 1054, 1490
BmrFI	CCNGG	9 cut(s)	247, 303, 517, 616, 671, 1290, 2019, 2370, 2495
BmrI	ACTGGG	1 cut(s)	67
BmsI	GCATC	8 cut(s)	71, 1198, 1235, 1344, 1375, 1605, 2268, 2446
BmuI	ACTGGG	1 cut(s)	67
BpiI	GAAGAC	3 cut(s)	130, 761, 1195
BpmI	CTGGAG	3 cut(s)	160, 1196, 2498
Bpu10I	CCTNAGC	1 cut(s)	128
BpuEI	CTTGAG	2 cut(s)	198, 798
BpuMI	CCSGG	2 cut(s)	247, 517
BsaAI	YACGTR	1 cut(s)	740
BsaHI	GRCGYC	2 cut(s)	122, 2544
BsaI	GGTCTC	2 cut(s)	417, 824
BsaJI	CCNNGG	9 cut(s)	187, 246, 264, 400, 670, 834, 1289, 2247, 2383
BsaWI	WCCGGW	1 cut(s)	419
BsaXI	ACNNNNNCTCC	6 cut(s)	315, 345, 958, 988, 2379, 2409
Bsc4I	CCNNNNNNNGG	7 cut(s)	109, 485, 532, 1258, 1499, 1825, 2024
Bse118I	RCCGGY	1 cut(s)	2438
Bse3DI	GCAATG	2 cut(s)	439, 885
BseBI	CCWGG	7 cut(s)	303, 616, 671, 1290, 2019, 2370, 2495
BseDI	CCNNGG	9 cut(s)	187, 246, 264, 400, 670, 834, 1289, 2247, 2383
BseGI	GGATG	8 cut(s)	1299, 1620, 1705, 1721, 1810, 2005, 2079, 2210
BseLI	CCNNNNNNNGG	7 cut(s)	109, 485, 532, 1258, 1499, 1825, 2024
BseMI	GCAATG	2 cut(s)	439, 885
BseMII	CTCAG	5 cut(s)	142, 915, 1796, 2244, 2331
BseRI	GAGGAG	2 cut(s)	704, 1259
BseSI	GKGCMC	1 cut(s)	1056
BseYI	CCCAGC	1 cut(s)	297
BsgI	GTGCAG	1 cut(s)	1538
Bsh1236I	CGCG	2 cut(s)	221, 593
BshFI	GGCC	8 cut(s)	113, 283, 405, 467, 1054, 1250, 1438, 2410
BshNI	GGYRCC	1 cut(s)	191
BsiHKAI	GWGCWC	3 cut(s)	851, 971, 2199
BsiHKCI	CYCGRG	1 cut(s)	1253
BsiSI	CCGG	5 cut(s)	171, 246, 420, 516, 2439
BslFI	GGGAC	1 cut(s)	109
BslI	CCNNNNNNNGG	7 cut(s)	109, 485, 532, 1258, 1499, 1825, 2024
BsmAI	GTCTC	9 cut(s)	121, 417, 497, 640, 824, 1172, 1407, 1554, 1658
BsmBI	CGTCTC	1 cut(s)	121
BsmFI	GGGAC	1 cut(s)	109
BsmI	GAATGC	1 cut(s)	444
BsnI	GGCC	8 cut(s)	113, 283, 405, 467, 1054, 1250, 1438, 2410
Bso31I	GGTCTC	2 cut(s)	417, 824
BsoBI	CYCGRG	1 cut(s)	1253
Bsp120I	GGGCCC	1 cut(s)	1052
Bsp1286I	GDGCHC	4 cut(s)	851, 971, 1056, 2199
Bsp1407I	TGTACA	1 cut(s)	1474
Bsp143I	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
Bsp19I	CCATGG	1 cut(s)	2247
Bsp68I	TCGCGA	1 cut(s)	593
BspANI	GGCC	8 cut(s)	113, 283, 405, 467, 1054, 1250, 1438, 2410
BspCNI	CTCAG	5 cut(s)	141, 916, 1795, 2243, 2330
BspFNI	CGCG	2 cut(s)	221, 593
BspHI	TCATGA	1 cut(s)	685
BspLI	GGNNCC	5 cut(s)	175, 193, 477, 1054, 1490
BspMAI	CTGCAG	1 cut(s)	1564
BspPI	GGATC	4 cut(s)	350, 728, 771, 1098
BspT107I	GGYRCC	1 cut(s)	191
BspTNI	GGTCTC	2 cut(s)	417, 824
BsrDI	GCAATG	2 cut(s)	439, 885
BsrFI	RCCGGY	1 cut(s)	2438
BsrGI	TGTACA	1 cut(s)	1474
BssAI	RCCGGY	1 cut(s)	2438
BssECI	CCNNGG	9 cut(s)	187, 246, 264, 400, 670, 834, 1289, 2247, 2383
BssMI	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
BssNI	GRCGYC	2 cut(s)	122, 2544
BssSI	CACGAG	1 cut(s)	1619
BssT1I	CCWWGG	4 cut(s)	264, 834, 2247, 2383
Bst2BI	CACGAG	1 cut(s)	1619
Bst2UI	CCWGG	7 cut(s)	303, 616, 671, 1290, 2019, 2370, 2495
Bst4CI	ACNGT	5 cut(s)	191, 508, 1143, 1962, 2504
Bst6I	CTCTTC	2 cut(s)	2185, 2461
BstACI	GRCGYC	2 cut(s)	122, 2544
BstAPI	GCANNNNNTGC	1 cut(s)	2456
BstAUI	TGTACA	1 cut(s)	1474
BstBAI	YACGTR	1 cut(s)	740
BstC8I	GCNNGC	8 cut(s)	484, 491, 495, 759, 851, 855, 1440, 2221
BstDEI	CTNAG	7 cut(s)	128, 387, 924, 1782, 1820, 2230, 2317
BstDSI	CCRYGG	3 cut(s)	187, 400, 2247
BstEII	GGTNACC	1 cut(s)	223
BstENI	CCTNNNNNAGG	1 cut(s)	1256
BstF5I	GGATG	8 cut(s)	1299, 1620, 1705, 1721, 1810, 2005, 2079, 2210
BstFNI	CGCG	2 cut(s)	221, 593
BstHHI	GCGC	1 cut(s)	414
BstKTI	GATC	9 cut(s)	358, 736, 766, 901, 952, 1093, 1132, 1269, 1583
BstMAI	GTCTC	9 cut(s)	121, 417, 497, 640, 824, 1172, 1407, 1554, 1658
BstMBI	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
BstNI	CCWGG	7 cut(s)	303, 616, 671, 1290, 2019, 2370, 2495
BstNSI	RCATGY	2 cut(s)	9, 549
BstPI	GGTNACC	1 cut(s)	223
BstSCI	CCNGG	9 cut(s)	245, 301, 515, 614, 669, 1288, 2017, 2368, 2493
BstSFI	CTRYAG	4 cut(s)	1104, 1339, 1560, 1764
BstSLI	GKGCMC	1 cut(s)	1056
BstUI	CGCG	2 cut(s)	221, 593
BstV2I	GAAGAC	3 cut(s)	130, 761, 1195
BstX2I	RGATCY	2 cut(s)	898, 1090
BstYI	RGATCY	2 cut(s)	898, 1090
BsuRI	GGCC	8 cut(s)	113, 283, 405, 467, 1054, 1250, 1438, 2410
BtgI	CCRYGG	3 cut(s)	187, 400, 2247
BtgZI	GCGATG	3 cut(s)	142, 223, 2311
BtrI	CACGTC	1 cut(s)	331
BtsCI	GGATG	8 cut(s)	1299, 1620, 1705, 1721, 1810, 2005, 2079, 2210
BtsI	GCAGTG	2 cut(s)	1557, 1902
BtsIMutI	CAGTG	3 cut(s)	1454, 1557, 1902
BtuMI	TCGCGA	1 cut(s)	593
Cac8I	GCNNGC	8 cut(s)	484, 491, 495, 759, 851, 855, 1440, 2221
CaiI	CAGNNNCTG	4 cut(s)	308, 1552, 1931, 2375
CciI	TCATGA	1 cut(s)	685
CfoI	GCGC	1 cut(s)	414
Cfr10I	RCCGGY	1 cut(s)	2438
CseI	GACGC	1 cut(s)	111
Csp6I	GTAC	4 cut(s)	1475, 1565, 1624, 2077
CviQI	GTAC	4 cut(s)	1475, 1565, 1624, 2077
DdeI	CTNAG	7 cut(s)	128, 387, 924, 1782, 1820, 2230, 2317
DpnI	GATC	9 cut(s)	357, 735, 765, 900, 951, 1092, 1131, 1268, 1582
DpnII	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
DriI	GACNNNNNGTC	1 cut(s)	1814
EaeI	YGGCCR	3 cut(s)	111, 403, 465
Eam1104I	CTCTTC	2 cut(s)	2185, 2461
Eam1105I	GACNNNNNGTC	1 cut(s)	1814
EarI	CTCTTC	2 cut(s)	2185, 2461
Ecl136II	GAGCTC	2 cut(s)	969, 2197
Eco130I	CCWWGG	4 cut(s)	264, 834, 2247, 2383
Eco24I	GRGCYC	3 cut(s)	971, 1056, 2199
Eco31I	GGTCTC	2 cut(s)	417, 824
Eco32I	GATATC	1 cut(s)	341
Eco47I	GGWCC	6 cut(s)	91, 1488, 1571, 1753, 2286, 2491
Eco53kI	GAGCTC	2 cut(s)	969, 2197
Eco57I	CTGAAG	2 cut(s)	902, 1941
Eco72I	CACGTG	1 cut(s)	740
Eco88I	CYCGRG	1 cut(s)	1253
Eco91I	GGTNACC	1 cut(s)	223
EcoICRI	GAGCTC	2 cut(s)	969, 2197
EcoNI	CCTNNNNNAGG	1 cut(s)	1256
EcoO109I	RGGNCCY	3 cut(s)	1052, 1249, 1571
EcoO65I	GGTNACC	1 cut(s)	223
EcoRII	CCWGG	7 cut(s)	301, 614, 669, 1288, 2017, 2368, 2493
EcoRV	GATATC	1 cut(s)	341
EcoT14I	CCWWGG	4 cut(s)	264, 834, 2247, 2383
EcoT38I	GRGCYC	3 cut(s)	971, 1056, 2199
ErhI	CCWWGG	4 cut(s)	264, 834, 2247, 2383
Esp3I	CGTCTC	1 cut(s)	121
FalI	AAGNNNNNCTT	2 cut(s)	2313, 2345
FaqI	GGGAC	1 cut(s)	109
FauI	CCCGC	1 cut(s)	807
FbaI	TGATCA	1 cut(s)	1266
FokI	GGATG	8 cut(s)	1306, 1627, 1708, 1712, 1797, 2012, 2086, 2197
FriOI	GRGCYC	3 cut(s)	971, 1056, 2199
FspBI	CTAG	7 cut(s)	953, 972, 1163, 1536, 1910, 2045, 2256
FspI	TGCGCA	1 cut(s)	413
GlaI	GCGC	1 cut(s)	413
GsaI	CCCAGC	1 cut(s)	301
GsuI	CTGGAG	3 cut(s)	160, 1196, 2498
HaeIII	GGCC	8 cut(s)	113, 283, 405, 467, 1054, 1250, 1438, 2410
HapII	CCGG	5 cut(s)	171, 246, 420, 516, 2439
HgaI	GACGC	1 cut(s)	111
HhaI	GCGC	1 cut(s)	414
Hin1I	GRCGYC	2 cut(s)	122, 2544
Hin6I	GCGC	1 cut(s)	412
HinP1I	GCGC	1 cut(s)	412
HincII	GTYRAC	2 cut(s)	2241, 2500
HindII	GTYRAC	2 cut(s)	2241, 2500
HindIII	AAGCTT	3 cut(s)	755, 816, 2221
HinfI	GANTC	7 cut(s)	459, 520, 1180, 1638, 1703, 1834, 2150
HpaI	GTTAAC	1 cut(s)	2500
HpaII	CCGG	5 cut(s)	171, 246, 420, 516, 2439
Hpy166II	GTNNAC	8 cut(s)	328, 538, 667, 677, 1477, 2015, 2241, 2500
Hpy188III	TCNNGA	9 cut(s)	89, 592, 686, 861, 934, 1175, 1466, 1574, 2066
Hpy8I	GTNNAC	8 cut(s)	328, 538, 667, 677, 1477, 2015, 2241, 2500
Hpy99I	CGWCG	4 cut(s)	236, 2204, 2546, 2549
HpyAV	CCTTC	7 cut(s)	1043, 1081, 1472, 1823, 1872, 1984, 2125
HpyCH4III	ACNGT	5 cut(s)	191, 508, 1143, 1962, 2504
HpyCH4IV	ACGT	6 cut(s)	153, 330, 739, 1676, 1716, 2544
HpyF3I	CTNAG	7 cut(s)	128, 387, 924, 1782, 1820, 2230, 2317
HpySE526I	ACGT	6 cut(s)	153, 330, 739, 1676, 1716, 2544
Hsp92I	GRCGYC	2 cut(s)	122, 2544
HspAI	GCGC	1 cut(s)	412
Ksp22I	TGATCA	1 cut(s)	1266
KspAI	GTTAAC	1 cut(s)	2500
Kzo9I	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
LmnI	GCTCC	4 cut(s)	20, 137, 179, 966
LweI	GCATC	8 cut(s)	71, 1198, 1235, 1344, 1375, 1605, 2268, 2446
MaeI	CTAG	7 cut(s)	953, 972, 1163, 1536, 1910, 2045, 2256
MaeII	ACGT	6 cut(s)	153, 330, 739, 1676, 1716, 2544
MaeIII	GTNAC	9 cut(s)	196, 223, 331, 974, 1075, 1300, 1454, 1993, 2206
MalI	GATC	9 cut(s)	357, 735, 765, 900, 951, 1092, 1131, 1268, 1582
MboI	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
MfeI	CAATTG	1 cut(s)	1494
MflI	RGATCY	2 cut(s)	898, 1090
MhlI	GDGCHC	4 cut(s)	851, 971, 1056, 2199
MlsI	TGGCCA	1 cut(s)	467
MluNI	TGGCCA	1 cut(s)	467
MlyI	GAGTC	3 cut(s)	1174, 1632, 1843
MmeI	TCCRAC	3 cut(s)	1065, 1390, 2135
Mox20I	TGGCCA	1 cut(s)	467
MroXI	GAANNNNTTC	1 cut(s)	1942
MscI	TGGCCA	1 cut(s)	467
MseI	TTAA	5 cut(s)	620, 1019, 1155, 1397, 2499
MslI	CAYNNNNRTG	2 cut(s)	26, 1082
Msp20I	TGGCCA	1 cut(s)	467
MspA1I	CMGCKG	3 cut(s)	164, 802, 2509
MspI	CCGG	5 cut(s)	171, 246, 420, 516, 2439
MspR9I	CCNGG	9 cut(s)	247, 303, 517, 616, 671, 1290, 2019, 2370, 2495
MunI	CAATTG	1 cut(s)	1494
Mva1269I	GAATGC	1 cut(s)	444
MvaI	CCWGG	7 cut(s)	303, 616, 671, 1290, 2019, 2370, 2495
MvnI	CGCG	2 cut(s)	221, 593
NciI	CCSGG	2 cut(s)	247, 517
NcoI	CCATGG	1 cut(s)	2247
NdeII	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
NlaIV	GGNNCC	5 cut(s)	175, 193, 477, 1054, 1490
NmeAIII	GCCGAG	1 cut(s)	98
NmuCI	GTSAC	5 cut(s)	196, 223, 331, 1075, 1993
NruI	TCGCGA	1 cut(s)	593
NsbI	TGCGCA	1 cut(s)	413
NspI	RCATGY	2 cut(s)	9, 549
PaeR7I	CTCGAG	1 cut(s)	1253
PagI	TCATGA	1 cut(s)	685
PciI	ACATGT	1 cut(s)	545
PcsI	WCGNNNNNNNCGW	1 cut(s)	1053
PctI	GAATGC	1 cut(s)	444
PdmI	GAANNNNTTC	1 cut(s)	1942
PfeI	GAWTC	4 cut(s)	459, 520, 1703, 2150
PflFI	GACNNNGTC	1 cut(s)	1170
PflMI	CCANNNNNTGG	3 cut(s)	532, 1499, 2024
PleI	GAGTC	3 cut(s)	1174, 1632, 1842
PmaCI	CACGTG	1 cut(s)	740
PmlI	CACGTG	1 cut(s)	740
PpsI	GAGTC	3 cut(s)	1174, 1632, 1842
Ppu21I	YACGTR	1 cut(s)	740
PpuMI	RGGWCCY	1 cut(s)	1571
PscI	ACATGT	1 cut(s)	545
PsiI	TTATAA	2 cut(s)	752, 1659
Psp124BI	GAGCTC	2 cut(s)	971, 2199
Psp5II	RGGWCCY	1 cut(s)	1571
Psp6I	CCWGG	7 cut(s)	301, 614, 669, 1288, 2017, 2368, 2493
PspCI	CACGTG	1 cut(s)	740
PspEI	GGTNACC	1 cut(s)	223
PspFI	CCCAGC	1 cut(s)	297
PspGI	CCWGG	7 cut(s)	301, 614, 669, 1288, 2017, 2368, 2493
PspN4I	GGNNCC	5 cut(s)	175, 193, 477, 1054, 1490
PspOMI	GGGCCC	1 cut(s)	1052
PspPPI	RGGWCCY	1 cut(s)	1571
PspXI	VCTCGAGB	1 cut(s)	1253
PstI	CTGCAG	1 cut(s)	1564
PstNI	CAGNNNCTG	4 cut(s)	308, 1552, 1931, 2375
PsuI	RGATCY	2 cut(s)	898, 1090
PsyI	GACNNNGTC	1 cut(s)	1170
PvuII	CAGCTG	1 cut(s)	2509
RruI	TCGCGA	1 cut(s)	593
RsaI	GTAC	4 cut(s)	1476, 1566, 1625, 2078
RsaNI	GTAC	4 cut(s)	1475, 1565, 1624, 2077
RseI	CAYNNNNRTG	2 cut(s)	26, 1082
SacI	GAGCTC	2 cut(s)	971, 2199
SaqAI	TTAA	5 cut(s)	620, 1019, 1155, 1397, 2499
Sau3AI	GATC	9 cut(s)	355, 733, 763, 898, 949, 1090, 1129, 1266, 1580
SchI	GAGTC	3 cut(s)	1174, 1632, 1843
ScrFI	CCNGG	9 cut(s)	247, 303, 517, 616, 671, 1290, 2019, 2370, 2495
SduI	GDGCHC	4 cut(s)	851, 971, 1056, 2199
SfaNI	GCATC	8 cut(s)	71, 1198, 1235, 1344, 1375, 1605, 2268, 2446
SfcI	CTRYAG	4 cut(s)	1104, 1339, 1560, 1764
Sfr274I	CTCGAG	1 cut(s)	1253
SinI	GGWCC	6 cut(s)	91, 1488, 1571, 1753, 2286, 2491
SlaI	CTCGAG	1 cut(s)	1253
SmiMI	CAYNNNNRTG	2 cut(s)	26, 1082
SmlI	CTYRAG	3 cut(s)	213, 813, 1253
SmoI	CTYRAG	3 cut(s)	213, 813, 1253
SspI	AATATT	1 cut(s)	2159
SspMI	CTAG	7 cut(s)	953, 972, 1163, 1536, 1910, 2045, 2256
SstI	GAGCTC	2 cut(s)	971, 2199
StyD4I	CCNGG	9 cut(s)	245, 301, 515, 614, 669, 1288, 2017, 2368, 2493
StyI	CCWWGG	4 cut(s)	264, 834, 2247, 2383
TaaI	ACNGT	5 cut(s)	191, 508, 1143, 1962, 2504
TaiI	ACGT	6 cut(s)	156, 333, 742, 1679, 1719, 2547
TaqI	TCGA	6 cut(s)	270, 1132, 1254, 1310, 2065, 2199
TatI	WGTACW	2 cut(s)	1474, 1564
TauI	GCSGC	4 cut(s)	164, 489, 1317, 2462
TfiI	GAWTC	4 cut(s)	459, 520, 1703, 2150
Tru1I	TTAA	5 cut(s)	620, 1019, 1155, 1397, 2499
Tru9I	TTAA	5 cut(s)	620, 1019, 1155, 1397, 2499
TscAI	CASTG	3 cut(s)	1454, 1564, 1902
TseFI	GTSAC	5 cut(s)	196, 223, 331, 1075, 1993
Tsp45I	GTSAC	5 cut(s)	196, 223, 331, 1075, 1993
TspDTI	ATGAA	8 cut(s)	437, 896, 1083, 1100, 1364, 1716, 2016, 2107
TspGWI	ACGGA	8 cut(s)	124, 658, 1369, 1437, 1712, 2012, 2202, 2226
TspRI	CASTG	3 cut(s)	1454, 1564, 1902
Tth111I	GACNNNGTC	1 cut(s)	1170
Van91I	CCANNNNNTGG	3 cut(s)	532, 1499, 2024
VpaK11BI	GGWCC	6 cut(s)	91, 1488, 1571, 1753, 2286, 2491
XagI	CCTNNNNNAGG	1 cut(s)	1256
XapI	RAATTY	3 cut(s)	41, 988, 1148
XceI	RCATGY	2 cut(s)	9, 549
XcmI	CCANNNNNNNNNTGG	1 cut(s)	677
XhoI	CTCGAG	1 cut(s)	1253
XmnI	GAANNNNTTC	1 cut(s)	1942
XspI	CTAG	7 cut(s)	953, 972, 1163, 1536, 1910, 2045, 2256
ZraI	GACGTC	1 cut(s)	2545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.