Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0010595

Physical Location & Seq

Genomic Coordinates Forward (+)

570735 .. 577026

Gene Structure 6292 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00277130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2241 bp

ATGCCCTCTGCCTCATGCCAAACCTCAACCGCAATGTTCGTCCACAACTTCCACCGTCTCAGCCTCCGCAACTCCCCCAAATTCCGATGCGTCTTGGACCAGATTGCCCCCAACTTGGCCGTCTCCTCCTCCCTCAGCTCCGTCTTCACCTCTGCCAACCTCGTCGCCGCCGCCGGCTCCGGCTCTCTCCACAGCGCCGTCACCTCCACCATCACTCAAGTCGCTGTCACCGCCGTCGCCATCGCCTCCGGGGCTTGTCTCTCCACTAAAGTTGATTTCTTGTGGCCCAAACTCGAAACGCAGCCAGGCTGTGATATAGTGCAAAAGGCTATTGCATTTGCCAAGAAGGCTCACCATGGCCAAGTACGCAAAACTGGAGACCCTTATTTAGCTGTTCAGACGGTTGTTGCTGGGATTCTCCATGATGTCGTTGATGACACATGTGAAAGTTTCAACCGCATAGAAGAAGAATTCGGCGATGACGTGGCCAAGCTTGTAGCTGGAGTTTCCAGGTTAAGTTACATAAACCAGCTTTTGCGGAGACACCGCAGGATAAATGTGAATGACGGGAGACTTGGTCACGAGGAGGCAAATAACTTACGAGTCATGCTCTTGGGCATGGTTGATGATCCACGTGTGGTGCTTATAAAGCTTGCAGATCGTCTTCACAATATGAGAACAATTTATGCTCTGCCACCGGCAAAAGCTCAAGCTGTTGCTAGGGAGACCTTGGTGATTTGGTGTTCTCTAGCATCCAGATTGGGTTTGTGGGCTATGAAAGCTGAACTGGAAGATCTGTGCTTTGCTGTTCTCCAGCCTCAAATGTTTAAGAATATGAGAGCTGATCTAGCTTCCATGTGGAGCTCTAGCAGCAAAGTAGGAAATTTGAAAAGAATATCTGCAAGATCCACCTCAAATGGAGGAAGCTCAGTTTCGGACAATGAAGGATCCATCAATGATGAAGATGTCACAACCATGAAGGATCTCTTGGAAGCCGTAGTACCTTTCGATGTATTGTTAGATCGGAGAAAACGGTCTAATTTTCTAAGTACTCTAGGACAAGATTTGGAGACTCATAAAAGACCAAAGGTTGTGCATGATGCTGGAATTGCTTTAGCATCTCTGGTAATTTGTGAGGAGGCACTTGAGCAGGAGTTGATCATATCAACTTCTTATGTTCCAGGGATGGAAGTAACTTTATCCAGCCGTTTAAAAAGTTTGTATAGCATCTACAGCAAGATGAAACGAAAAGATGTCAGCATCAATAAAGTTTATGATGCCCGTGCATTAAGGGTAGTTGTTGGAGACAAGAATGGAACTTTGCATGGACCTGCTGTCCAATGTTGCTACAGTCTTCTCGACACTGTGCACAAGCATTGGACACCAATTGATGGTGAGTTTGATGATTACATTGTCAATCCAAAGCCTAGTGGCTATCAGTCTCTGCACACTGCAGTACAGGGTCCTGACAGATCACCTTTGGAAGTTCAAATACGAACGCAGAAGCATAGTATGTTGAAAATTGGGCATCCAGTTCTTAGAGTGGATGGAAGTCATCTTCTTGCTGCAGTTGTCATTAGAGTAGAGAAGGATGGAAGGGAATTACTCGTTGCCGTGAGCTTTGGACTAGAAGCTTCAGAAGCAGTCGCTGATAGAAAATATTCTTTTCAAAAAACAACGGTTTCTGATGAATGGTGGTGTGAACCAGGACATGGGGATTGGTGCACTTGCCTAGAGAAATATACACTTTGTCGAGATGGTATGTACCACAAGGAAGATCAGTTTGGGAGATTACTGCCGACATTCATTCAGGTGATTGATTTGACAGATGAAGAAGAATCTGAATATTGGGCTGTTGTTTCTGCTATCTTTGAGGGAAGACAGCTTGATTACATTACACCTACGCCAAGATTTAACTCAGTTACATCAACTTCCATGGAAACTAGCATCAATAACAAGGTGCATTTGCTAAGGACAATGCTACGGTGGGAAGAGCAATTACGGTCTGAAGCAATTTATGGAAATGGACAAGCCATGTATCGACGGGGTTCTGTTGTCCTTGGGGAAGTGGTGATTATATGTTGGCCGCATGGCGAAATAATGAGGTTGACAACAGGCAGCACCGCCGCAGATGCTGCTAGAAGGAAAGGGCTAGACGGAAAACTGGTTTTGGTTAATGGTCAGCTAGTGTTGCCCAATACAAAGCTCACAGATGGTGATGTGGTTGAAGTCAGAGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009259 GO:0009605 GO:0009987 GO:0009991 GO:0015969 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0033865 GO:0033875 GO:0034032 GO:0034035 GO:0034641 GO:0042278 GO:0042578 GO:0042594 GO:0044237 GO:0044238 GO:0044281 GO:0044464 GO:0046128 GO:0046483 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:1901068 GO:1901135 GO:1901360 GO:1901564 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00230 map00230

Pfam Domains

Protein Families

HD_4 RelA_SpoT TGS

Protein Analysis

746

Amino Acids

82.48

Weight (kDa)

6.89

Isoelectric Point (pI)

34.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HD_4	PF13328	132 - 257	7.8e-36	HD domain
RelA_SpoT	PF04607	402 - 504	3e-26	Region found in RelA / SpoT proteins
DUF7589	PF24500	501 - 626	1.3e-62	Domain of unknown function (DUF7589)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010595)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33820 FvH4_7g33820
malus_domestica	MD07G1306500.v1.1
prunus_persica	Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1
pyrus_communis	pycom07g27950
rosa_chinensis	RchiOBHm_Chr1g0383931
rosa_laevigata	RLG00000026085
rosa_multiflora	Rmu_sc0005139.1_g000026
rosa_roxburghii	Rroxscaffold_4G00277130
rosa_rugosa	Rorug01G0443100 Rorug01G0443200 Rorug01G0443400.1 Rorug01G0443500.1
rosa_samantha	Rh1AG469100 Rh1CG439600 Rh1DG459600
rosa_wichuraiana	Rw1G040330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	647
Acc36I	ACCTGC	1 cut(s)	1339
AccB7I	CCANNNNNTGG	2 cut(s)	1391, 1712
AclWI	GGATC	5 cut(s)	623, 900, 942, 955, 990
AcoI	YGGCCR	4 cut(s)	117, 358, 486, 2084
AcsI	RAATTY	3 cut(s)	80, 470, 883
AcuI	CTGAAG	2 cut(s)	1620, 2028
AcvI	CACGTG	1 cut(s)	635
AfaI	GTAC	5 cut(s)	366, 1002, 1051, 1458, 1766
AfiI	CCNNNNNNNGG	3 cut(s)	115, 1391, 1712
AflIII	ACRYGT	2 cut(s)	440, 634
AgsI	TTSAA	6 cut(s)	454, 889, 1490, 1519, 1670, 2228
AhdI	GACNNNNNGTC	1 cut(s)	1334
AjiI	CACGTC	1 cut(s)	484
AjnI	CCWGG	4 cut(s)	304, 509, 1180, 1705
AjuI	GAANNNNNNNTTGG	4 cut(s)	971, 1003, 1767, 1799
Alw21I	GWGCWC	3 cut(s)	866, 1371, 1727
Alw44I	GTGCAC	2 cut(s)	1367, 1723
AlwI	GGATC	5 cut(s)	623, 900, 942, 955, 990
AlwNI	CAGNNNCTG	3 cut(s)	1444, 1649, 2135
AoxI	GGCC	5 cut(s)	117, 284, 358, 486, 2084
ApaLI	GTGCAC	2 cut(s)	1367, 1723
ApeKI	GCWGC	5 cut(s)	301, 870, 1565, 2118, 2135
ApoI	RAATTY	3 cut(s)	80, 470, 883
Asp700I	GAANNNNTTC	1 cut(s)	1660
AspLEI	GCGC	1 cut(s)	197
AspS9I	GGNCC	4 cut(s)	97, 285, 1328, 1463
AsuC2I	CCSGG	1 cut(s)	250
AvaII	GGWCC	3 cut(s)	97, 1328, 1463
BaeGI	GKGCMC	2 cut(s)	1371, 1727
BalI	TGGCCA	2 cut(s)	360, 488
BamHI	GGATCC	1 cut(s)	947
BanII	GRGCYC	1 cut(s)	866
BarI	GAAGNNNNNNTAC	6 cut(s)	984, 1016, 1476, 1508, 1983, 2015
BauI	CACGAG	1 cut(s)	581
BbrPI	CACGTG	1 cut(s)	635
BbsI	GAAGAC	4 cut(s)	136, 656, 1346, 1885
Bbv12I	GWGCWC	3 cut(s)	866, 1371, 1727
BbvCI	CCTCAGC	1 cut(s)	134
BbvI	GCAGC	5 cut(s)	313, 882, 1552, 2122, 2130
BccI	CCATC	9 cut(s)	218, 248, 959, 1180, 1385, 1541, 1586, 1751, 2207
BceAI	ACGGC	6 cut(s)	104, 182, 218, 980, 1191, 1598
BciT130I	CCWGG	4 cut(s)	306, 511, 1182, 1707
BclI	TGATCA	1 cut(s)	1158
BcnI	CCSGG	1 cut(s)	250
BfmI	CTRYAG	4 cut(s)	1231, 1348, 1452, 1566
BfoI	RGCGCY	1 cut(s)	198
BfuAI	ACCTGC	1 cut(s)	1339
BglI	GCCNNNNNGGC	2 cut(s)	251, 347
BglII	AGATCT	1 cut(s)	793
BisI	GCNGC	9 cut(s)	168, 171, 302, 871, 1566, 2087, 2119, 2127, 2136
BlsI	GCNGC	9 cut(s)	169, 172, 303, 872, 1567, 2088, 2120, 2128, 2137
BmcAI	AGTACT	1 cut(s)	1051
Bme1390I	CCNGG	5 cut(s)	250, 306, 511, 1182, 1707
Bme18I	GGWCC	3 cut(s)	97, 1328, 1463
BmeRI	GACNNNNNGTC	1 cut(s)	1334
BmgBI	CACGTC	1 cut(s)	484
BmgT120I	GGNCC	4 cut(s)	97, 285, 1328, 1463
BmiI	GGNNCC	3 cut(s)	178, 949, 1464
BmrFI	CCNGG	5 cut(s)	250, 306, 511, 1182, 1707
BpiI	GAAGAC	4 cut(s)	136, 656, 1346, 1885
BplI	GAGNNNNNCTC	2 cut(s)	594, 626
BpmI	CTGGAG	3 cut(s)	396, 522, 797
Bpu10I	CCTNAGC	2 cut(s)	134, 1970
BpuEI	CTTGAG	3 cut(s)	201, 693, 1166
BpuMI	CCSGG	1 cut(s)	250
BsaAI	YACGTR	1 cut(s)	635
BsaI	GGTCTC	2 cut(s)	372, 719
BsaJI	CCNNGG	6 cut(s)	249, 355, 729, 1181, 1935, 2059
BsaXI	ACNNNNNCTCC	2 cut(s)	562, 592
Bsc4I	CCNNNNNNNGG	3 cut(s)	115, 1391, 1712
Bse118I	RCCGGY	2 cut(s)	173, 697
Bse1I	ACTGG	4 cut(s)	379, 792, 1532, 2169
Bse3DI	GCAATG	1 cut(s)	39
BseBI	CCWGG	4 cut(s)	306, 511, 1182, 1707
BseDI	CCNNGG	6 cut(s)	249, 355, 729, 1181, 1935, 2059
BseGI	GGATG	5 cut(s)	752, 1191, 1528, 1552, 1597
BseLI	CCNNNNNNNGG	3 cut(s)	115, 1391, 1712
BseMI	GCAATG	1 cut(s)	39
BseMII	CTCAG	4 cut(s)	73, 148, 942, 1932
BseNI	ACTGG	4 cut(s)	379, 792, 1532, 2169
BseRI	GAGGAG	4 cut(s)	115, 118, 599, 1151
BseSI	GKGCMC	2 cut(s)	1371, 1727
BseXI	GCAGC	5 cut(s)	313, 882, 1552, 2122, 2130
BseYI	CCCAGC	1 cut(s)	410
BsgI	GTGCAG	1 cut(s)	1430
BshFI	GGCC	5 cut(s)	119, 286, 360, 488, 2086
BsiHKAI	GWGCWC	3 cut(s)	866, 1371, 1727
BsiSI	CCGG	4 cut(s)	174, 180, 249, 698
BslI	CCNNNNNNNGG	3 cut(s)	115, 1391, 1712
BsmBI	CGTCTC	2 cut(s)	62, 127
BsnI	GGCC	5 cut(s)	119, 286, 360, 488, 2086
Bso31I	GGTCTC	2 cut(s)	372, 719
Bsp1286I	GDGCHC	3 cut(s)	866, 1371, 1727
Bsp19I	CCATGG	2 cut(s)	355, 1935
BspANI	GGCC	5 cut(s)	119, 286, 360, 488, 2086
BspCNI	CTCAG	4 cut(s)	72, 147, 941, 1931
BspLI	GGNNCC	3 cut(s)	178, 949, 1464
BspMAI	CTGCAG	2 cut(s)	1456, 1570
BspMI	ACCTGC	1 cut(s)	1339
BspPI	GGATC	5 cut(s)	623, 900, 942, 955, 990
BspQI	GCTCTTC	1 cut(s)	1986
BspTNI	GGTCTC	2 cut(s)	372, 719
BsrDI	GCAATG	1 cut(s)	39
BsrFI	RCCGGY	2 cut(s)	173, 697
BsrI	ACTGG	4 cut(s)	379, 792, 1532, 2169
BssAI	RCCGGY	2 cut(s)	173, 697
BssECI	CCNNGG	6 cut(s)	249, 355, 729, 1181, 1935, 2059
BssSI	CACGAG	1 cut(s)	581
BssT1I	CCWWGG	4 cut(s)	355, 729, 1935, 2059
Bst2BI	CACGAG	1 cut(s)	581
Bst2UI	CCWGG	4 cut(s)	306, 511, 1182, 1707
Bst4CI	ACNGT	8 cut(s)	56, 403, 1035, 1352, 1366, 1681, 1986, 2004
Bst6I	CTCTTC	1 cut(s)	1986
BstAPI	GCANNNNNTGC	1 cut(s)	2135
BstBAI	YACGTR	1 cut(s)	635
BstC8I	GCNNGC	2 cut(s)	175, 654
BstDEI	CTNAG	7 cut(s)	59, 134, 928, 1046, 1538, 1918, 1970
BstDSI	CCRYGG	2 cut(s)	355, 1935
BstF5I	GGATG	5 cut(s)	752, 1191, 1528, 1552, 1597
BstH2I	RGCGCY	1 cut(s)	198
BstHHI	GCGC	1 cut(s)	197
BstNI	CCWGG	4 cut(s)	306, 511, 1182, 1707
BstNSI	RCATGY	1 cut(s)	444
BstSCI	CCNGG	5 cut(s)	248, 304, 509, 1180, 1705
BstSFI	CTRYAG	4 cut(s)	1231, 1348, 1452, 1566
BstSLI	GKGCMC	2 cut(s)	1371, 1727
BstV1I	GCAGC	5 cut(s)	313, 882, 1552, 2122, 2130
BstV2I	GAAGAC	4 cut(s)	136, 656, 1346, 1885
BstX2I	RGATCY	4 cut(s)	793, 905, 947, 982
BstYI	RGATCY	4 cut(s)	793, 905, 947, 982
BsuRI	GGCC	5 cut(s)	119, 286, 360, 488, 2086
BtgI	CCRYGG	2 cut(s)	355, 1935
BtgZI	GCGATG	2 cut(s)	226, 492
BtrI	CACGTC	1 cut(s)	484
BtsCI	GGATG	5 cut(s)	752, 1191, 1528, 1552, 1597
BtsI	GCAGTG	1 cut(s)	1449
BtsIMutI	CAGTG	2 cut(s)	1362, 1449
BveI	ACCTGC	1 cut(s)	1339
Cac8I	GCNNGC	2 cut(s)	175, 654
CaiI	CAGNNNCTG	3 cut(s)	1444, 1649, 2135
CfoI	GCGC	1 cut(s)	197
Cfr10I	RCCGGY	2 cut(s)	173, 697
Cfr13I	GGNCC	4 cut(s)	97, 285, 1328, 1463
CseI	GACGC	1 cut(s)	79
Csp6I	GTAC	5 cut(s)	365, 1001, 1050, 1457, 1765
CspCI	CAANNNNNGTGG	2 cut(s)	253, 288
CviQI	GTAC	5 cut(s)	365, 1001, 1050, 1457, 1765
DdeI	CTNAG	7 cut(s)	59, 134, 928, 1046, 1538, 1918, 1970
DraI	TTTAAA	1 cut(s)	1212
DriI	GACNNNNNGTC	1 cut(s)	1334
EaeI	YGGCCR	4 cut(s)	117, 358, 486, 2084
Eam1104I	CTCTTC	1 cut(s)	1986
Eam1105I	GACNNNNNGTC	1 cut(s)	1334
EarI	CTCTTC	1 cut(s)	1986
Ecl136II	GAGCTC	1 cut(s)	864
Eco130I	CCWWGG	4 cut(s)	355, 729, 1935, 2059
Eco24I	GRGCYC	1 cut(s)	866
Eco31I	GGTCTC	2 cut(s)	372, 719
Eco47I	GGWCC	3 cut(s)	97, 1328, 1463
Eco53kI	GAGCTC	1 cut(s)	864
Eco57I	CTGAAG	2 cut(s)	1620, 2028
Eco72I	CACGTG	1 cut(s)	635
EcoICRI	GAGCTC	1 cut(s)	864
EcoO109I	RGGNCCY	1 cut(s)	1463
EcoRI	GAATTC	1 cut(s)	470
EcoRII	CCWGG	4 cut(s)	304, 509, 1180, 1705
EcoT14I	CCWWGG	4 cut(s)	355, 729, 1935, 2059
EcoT38I	GRGCYC	1 cut(s)	866
ErhI	CCWWGG	4 cut(s)	355, 729, 1935, 2059
Esp3I	CGTCTC	2 cut(s)	62, 127
FalI	AAGNNNNNCTT	2 cut(s)	971, 1003
FbaI	TGATCA	1 cut(s)	1158
Fnu4HI	GCNGC	9 cut(s)	168, 171, 302, 871, 1566, 2087, 2119, 2127, 2136
FokI	GGATG	5 cut(s)	739, 1198, 1515, 1559, 1604
FriOI	GRGCYC	1 cut(s)	866
Fsp4HI	GCNGC	9 cut(s)	168, 171, 302, 871, 1566, 2087, 2119, 2127, 2136
GlaI	GCGC	1 cut(s)	196
GluI	GCNGC	9 cut(s)	168, 171, 302, 871, 1566, 2087, 2119, 2127, 2136
GsaI	CCCAGC	1 cut(s)	414
GsuI	CTGGAG	3 cut(s)	396, 522, 797
HaeII	RGCGCY	1 cut(s)	198
HaeIII	GGCC	5 cut(s)	119, 286, 360, 488, 2086
HapII	CCGG	4 cut(s)	174, 180, 249, 698
HgaI	GACGC	1 cut(s)	79
HhaI	GCGC	1 cut(s)	197
Hin6I	GCGC	1 cut(s)	195
HinP1I	GCGC	1 cut(s)	195
HincII	GTYRAC	1 cut(s)	2109
HindII	GTYRAC	1 cut(s)	2109
HindIII	AAGCTT	3 cut(s)	491, 650, 1632
HinfI	GANTC	4 cut(s)	415, 603, 1072, 1838
HpaII	CCGG	4 cut(s)	174, 180, 249, 698
Hpy166II	GTNNAC	5 cut(s)	43, 1369, 1703, 1725, 2109
Hpy188I	TCNGA	9 cut(s)	86, 399, 937, 1026, 1639, 1687, 1843, 2008, 2234
Hpy188III	TCNNGA	5 cut(s)	581, 756, 1358, 1466, 1754
Hpy8I	GTNNAC	5 cut(s)	43, 1369, 1703, 1725, 2109
Hpy99I	CGWCG	3 cut(s)	167, 239, 2046
HpyAV	CCTTC	6 cut(s)	340, 938, 973, 1582, 1590, 2136
HpyCH4III	ACNGT	8 cut(s)	56, 403, 1035, 1352, 1366, 1681, 1986, 2004
HpyCH4IV	ACGT	2 cut(s)	483, 634
HpyF3I	CTNAG	7 cut(s)	59, 134, 928, 1046, 1538, 1918, 1970
HpySE526I	ACGT	2 cut(s)	483, 634
HspAI	GCGC	1 cut(s)	195
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Ksp22I	TGATCA	1 cut(s)	1158
LguI	GCTCTTC	1 cut(s)	1986
LmnI	GCTCC	3 cut(s)	143, 182, 861
Lsp1109I	GCAGC	5 cut(s)	313, 882, 1552, 2122, 2130
MaeII	ACGT	2 cut(s)	483, 634
MaeIII	GTNAC	7 cut(s)	199, 226, 518, 578, 967, 1192, 1921
MfeI	CAATTG	1 cut(s)	1386
MflI	RGATCY	4 cut(s)	793, 905, 947, 982
MhlI	GDGCHC	3 cut(s)	866, 1371, 1727
MlsI	TGGCCA	2 cut(s)	360, 488
MluNI	TGGCCA	2 cut(s)	360, 488
MlyI	GAGTC	2 cut(s)	612, 1066
MmeI	TCCRAC	1 cut(s)	1282
Mox20I	TGGCCA	2 cut(s)	360, 488
MroNI	GCCGGC	1 cut(s)	173
MroXI	GAANNNNTTC	1 cut(s)	1660
MscI	TGGCCA	2 cut(s)	360, 488
MseI	TTAA	6 cut(s)	515, 828, 1211, 1289, 1914, 2175
MslI	CAYNNNNRTG	2 cut(s)	974, 1811
Msp20I	TGGCCA	2 cut(s)	360, 488
MspI	CCGG	4 cut(s)	174, 180, 249, 698
MspR9I	CCNGG	5 cut(s)	250, 306, 511, 1182, 1707
MunI	CAATTG	1 cut(s)	1386
MvaI	CCWGG	4 cut(s)	306, 511, 1182, 1707
NaeI	GCCGGC	1 cut(s)	175
NciI	CCSGG	1 cut(s)	250
NcoI	CCATGG	2 cut(s)	355, 1935
NgoMIV	GCCGGC	1 cut(s)	173
NlaIV	GGNNCC	3 cut(s)	178, 949, 1464
NmuCI	GTSAC	4 cut(s)	199, 226, 578, 967
NspI	RCATGY	1 cut(s)	444
PciI	ACATGT	1 cut(s)	440
PciSI	GCTCTTC	1 cut(s)	1986
PcsI	WCGNNNNNNNCGW	1 cut(s)	480
PdiI	GCCGGC	1 cut(s)	175
PdmI	GAANNNNTTC	1 cut(s)	1660
PfeI	GAWTC	2 cut(s)	415, 1838
PflFI	GACNNNGTC	1 cut(s)	576
PflMI	CCANNNNNTGG	2 cut(s)	1391, 1712
PkrI	GCNGC	9 cut(s)	169, 172, 303, 872, 1567, 2088, 2120, 2128, 2137
PleI	GAGTC	2 cut(s)	611, 1066
PmaCI	CACGTG	1 cut(s)	635
PmlI	CACGTG	1 cut(s)	635
PpsI	GAGTC	2 cut(s)	611, 1066
Ppu21I	YACGTR	1 cut(s)	635
PpuMI	RGGWCCY	1 cut(s)	1463
PscI	ACATGT	1 cut(s)	440
PsiI	TTATAA	1 cut(s)	647
Psp124BI	GAGCTC	1 cut(s)	866
Psp5II	RGGWCCY	1 cut(s)	1463
Psp6I	CCWGG	4 cut(s)	304, 509, 1180, 1705
PspCI	CACGTG	1 cut(s)	635
PspFI	CCCAGC	1 cut(s)	410
PspGI	CCWGG	4 cut(s)	304, 509, 1180, 1705
PspN4I	GGNNCC	3 cut(s)	178, 949, 1464
PspPI	GGNCC	4 cut(s)	97, 285, 1328, 1463
PspPPI	RGGWCCY	1 cut(s)	1463
PstI	CTGCAG	2 cut(s)	1456, 1570
PstNI	CAGNNNCTG	3 cut(s)	1444, 1649, 2135
PsuI	RGATCY	4 cut(s)	793, 905, 947, 982
PsyI	GACNNNGTC	1 cut(s)	576
RsaI	GTAC	5 cut(s)	366, 1002, 1051, 1458, 1766
RsaNI	GTAC	5 cut(s)	365, 1001, 1050, 1457, 1765
RseI	CAYNNNNRTG	2 cut(s)	974, 1811
SacI	GAGCTC	1 cut(s)	866
SapI	GCTCTTC	1 cut(s)	1986
SaqAI	TTAA	6 cut(s)	515, 828, 1211, 1289, 1914, 2175
SatI	GCNGC	9 cut(s)	168, 171, 302, 871, 1566, 2087, 2119, 2127, 2136
Sau96I	GGNCC	4 cut(s)	97, 285, 1328, 1463
ScaI	AGTACT	1 cut(s)	1051
SchI	GAGTC	2 cut(s)	612, 1066
ScrFI	CCNGG	5 cut(s)	250, 306, 511, 1182, 1707
SduI	GDGCHC	3 cut(s)	866, 1371, 1727
SfcI	CTRYAG	4 cut(s)	1231, 1348, 1452, 1566
SinI	GGWCC	3 cut(s)	97, 1328, 1463
SmiMI	CAYNNNNRTG	2 cut(s)	974, 1811
SmlI	CTYRAG	3 cut(s)	216, 708, 1145
SmoI	CTYRAG	3 cut(s)	216, 708, 1145
SspI	AATATT	2 cut(s)	1661, 1847
SstI	GAGCTC	1 cut(s)	866
StyD4I	CCNGG	5 cut(s)	248, 304, 509, 1180, 1705
StyI	CCWWGG	4 cut(s)	355, 729, 1935, 2059
TaaI	ACNGT	8 cut(s)	56, 403, 1035, 1352, 1366, 1681, 1986, 2004
TaiI	ACGT	2 cut(s)	486, 637
TaqI	TCGA	5 cut(s)	294, 1008, 1359, 1753, 2041
TatI	WGTACW	2 cut(s)	1049, 1456
TauI	GCSGC	4 cut(s)	170, 173, 2089, 2129
TfiI	GAWTC	2 cut(s)	415, 1838
Tru1I	TTAA	6 cut(s)	515, 828, 1211, 1289, 1914, 2175
Tru9I	TTAA	6 cut(s)	515, 828, 1211, 1289, 1914, 2175
TscAI	CASTG	2 cut(s)	1369, 1456
TseFI	GTSAC	4 cut(s)	199, 226, 578, 967
TseI	GCWGC	5 cut(s)	301, 870, 1565, 2118, 2135
Tsp45I	GTSAC	4 cut(s)	199, 226, 578, 967
TspDTI	ATGAA	8 cut(s)	791, 957, 975, 992, 1256, 1704, 1795, 1845
TspGWI	ACGGA	2 cut(s)	130, 2172
TspRI	CASTG	2 cut(s)	1369, 1456
Tth111I	GACNNNGTC	1 cut(s)	576
Van91I	CCANNNNNTGG	2 cut(s)	1391, 1712
VneI	GTGCAC	2 cut(s)	1367, 1723
VpaK11BI	GGWCC	3 cut(s)	97, 1328, 1463
XapI	RAATTY	3 cut(s)	80, 470, 883
XceI	RCATGY	1 cut(s)	444
XmnI	GAANNNNTTC	1 cut(s)	1660
ZrmI	AGTACT	1 cut(s)	1051

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.