Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0010595

Physical Location & Seq

Genomic Coordinates Forward (+)

406538 .. 415290

Gene Structure 8753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026085

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3120 bp

ATGGGTCAAGCTTTTCGGAAGCTCTTCGATGCCTTCTTCGGAAACACTGAGATGCGAGTTGTGATGCTTGGACTAGATGCGGCTGGTAAAACAACCATCCTTTACAAACTGCATATTGGAGAAGTCTTGTCAACTGTTCCTACAATAGGTTTTAATGTGGAAAAAGTTCAGTACAAGAATGTGATGTTCACAGTTTGGGATGTTGGTGGGCAAGAGAAACTAAGGCCGCTCTGGAGGCATTACTTTAATAATACGGATGGACTGATTTATGTCGTTGACTCCTTGGACAGGGAGAGAATTGGACGAGCCAAGGCAGAATTTCAGGCCATAATCAGTGATCCGTTTATGCTCAACAGTGTCATCTTGGTGTTTGCAAACAAACAGGATATGAAAGGAGCTATGTCCCCAATGGAATGGGCACCTCATCGTTCTAATAGTCGAGGTTTATCTGGGTTTTTTGGGACTGTTCACTTGATAGGAGCTGGCGAGTTGCGCATCCAAACAAAACAACCAACAAGGCAAGAACTCTTGGCTTTGGCTAGCAACACATGCAAAAATAGCAAAGCAATGCCCTCATGCTCATGCCAAACTTCAACCTCCTCCTCCGCCATGTTCGTCCACAACTTCCACCGTCTCAGCCTCCGCAACTCCCCCAAATTCCGATGCGTCTTGGACCAGATTGCCCCCAACTTGGCCGTCTCCTCCTCCCTCAGCTCCGTCTTCACCTCTGCCAACCTCGTCGCCGCGGCCGCCGCCTCCGGCTCCGGCTCTCTCCACAGCGCCGTCACCTCCACCATCACTCAAGTCGCTGTCACCGCCGTCGCCATCGCCTCCGGGGCTTGTCTCTCCACTAAGGTTGATTTCTTGTGGCCCAAACTCGAAACGCAGCCAGGCTGTGATTTAGTGGAGGGAGTAGACGTGACTGGATATCCTATATTCAGTGATCCAAAGGTGCAAAAAGCTATTGCATTTGCCAAGAAGGCTCACCATGGCCAACTACGCAAAACCGGAGACCCTTATTTAGTCCATTGCATCCATACAGGAAGAATCTTGGCCATGTTGGTTCCTTCAAGTGGCGAACGAGCTGTTCAGACGGTTGTTGCTGGGATTCTCCACGATGTCGTTGATGACACGTGTGAAAGTTTCAACCGCATAGAAGAAGAATTTGGCGATGATGTAGCCAAGCTCGTAGCTGGAGTTTCCAGGTTAAGTTACATAAACCAGCTTTTGCGGAGACACCGCAGGATAAATGTGAATGACGGGAGACTTGGTCACGAGGAGGCAAATAACTTACGAGTCATGCTCTTGGGCATGGTTGATGATCCACGTGTAGTGCTTATAAAGCTTGCAGATCGTCTTCACAATATGAGAACAATTTACGCTCTGCCTCCGGCAAAAGCTCAAGCTGTTGCTAGGGAGACCTTGGTGATTTGGTGTTCTCTAGCATCCAGATTGGGTTTGTGGGCTATGAAAGCTGAACTGGAAGATCTGTGCTTTGCTGTTCTTCAGCCTCAAATGTTTAAGAACATGAGAGCTGATCTAGCTTCCATGTGGAGCTCTAGCAGCAAAGTAGGAAATTTGAAAAGAATATCTGCAAGATCCACCTTAAATGGAGGAAGCTCAGTTTCAGACAATGAAGGATCCATGAATGATGAAGATGTCACAACCATGAAGGATCTCTTGGAAGCCGTAGTACCTTTCGATGTCTTGTTAGATCGGAGAAAACGGTCTAATTTTCTAAGTACTCTAGAACAAGATTTGGAGACTCATAAAAGACCAAAGGTTGTGCATGATGCTGGAATTGCTTTAGCATCTCTGGTAATTTGTGAGGAGGCACTTGAGCAGGAGTTGATCATATCAACTTCTTATGTTCCAGGGATGGAAGTAACTTTATCCAGCCGCTTAAAAAGTTTGTATAGCATCTACAGCAAGATGAAACGAAAAGATGTCAGCATCAATAAAGTTTATGATGCCCGTGCATTAAGGGTAGTTGTTGGAGACAAGAATGGAACTTTGCATGGACCTGCTGTCCAATGTTGCTACAGTCTTCTCGACACTGTGCACAAGCATTGGACACCAATTGATGGTGAGTTTGATGATTACATTGTCAATCCAAAGCCTAGTGGCTATCAGTCTCTGCACACTGCAGTACAAGGTCCTGACAGATCACCTTTGGAAGTTCAAATACGAACACAGAGGATGCACGAGTATGCAGAACATGGACTTGCAGCACACTGGCTTTATAAAGAGACTGGAAACAAGCTGTCTGACAGCAGTAGCACTGATGAATCTGAAATAGATGCAACATCCTTTATGGACGACCAAAATGCTAGAGAAATTGATTTCTTTCAGAAGTTTAGTATGTTGAAAATTGGACATCCAGTTCTTAGAGTGGATGGAAGTCATCTTCTTGCTGCAGTTGTCATTAGAGTAGAGAAGGATGGAAGGGAATTACTCGTTGCCGTGAGCTTTGGACTAGAAGCTTCAGAAGCAGTCGCTGATAGAAAATATTCTTTTCAAAAACAACGGTGGGAAGCCTATGCAAGGTTATATAAGAAGGTTTCTGATGAGTGGTGGTGTGAACCAGGACATGGGGATTGGTGCACTTGCCTAGAGAAATATACACTTTGTCGAGACGGTATGTACCACAAGGAAGATCAGTTTGGGAGATTACTGCCAACATTCATTCAGGTGATTGATTTGACAGATGAAGAAGAATCTGAATATTGGGCTGTTGTTTCTGCTATCTTTGAGGGAAAACAGCTTGATTACATTACACCTACGCCAAGATTTAACTCAGTTACATCAACTTCCATGGAAACTAGCATCAATAACAAGGTGCATTTGCTAAGGACAATGCTACGGTGGGAAGAGCAATTACGGTCTGAAGCAATTTATGGAAATGGACAAGCCATGTATCGACGGGGTTCTGTTGTCCTTGGGGAAGTGGTGATTATATGTTGGCCGCATGGCGAAATAATGAGGTTGACAACAGGCAGCACCGCCGCAGATGCTGCTAGAAGGAAAGGACTAGACGGAAAACTGGTTTTGGTTAATGGTCAGCTGGTGTTGCCCAATACAAAGCTCACAGATGGTGATGTGGTTGAAGTCAGAGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009259 GO:0009605 GO:0009987 GO:0009991 GO:0015969 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0033865 GO:0033875 GO:0034032 GO:0034035 GO:0034641 GO:0042278 GO:0042578 GO:0042594 GO:0044237 GO:0044238 GO:0044281 GO:0044464 GO:0046128 GO:0046483 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:1901068 GO:1901135 GO:1901360 GO:1901564 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00230 map00230

Pfam Domains

Protein Families

HD_4 RelA_SpoT TGS

Protein Analysis

1040

Amino Acids

115.59

Weight (kDa)

6.88

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	6 - 139	5.6e-55	ADP-ribosylation factor family
SRPRB	PF09439	17 - 136	4.5e-09	Signal recognition particle receptor beta subunit
Roc	PF08477	19 - 129	6.7e-11	Ras of Complex, Roc, domain of DAPkinase
Ras	PF00071	19 - 132	1.2e-08	Ras family
Gtr1_RagA	PF04670	20 - 129	9.5e-07	Gtr1/RagA G protein conserved region
G-alpha	PF00503	44 - 130	6.9e-09	G-protein alpha subunit
HD_4	PF13328	321 - 488	9.4e-44	HD domain
HD	PF01966	341 - 456	5.9e-09	HD domain
RelA_SpoT	PF04607	633 - 752	2e-35	Region found in RelA / SpoT proteins
DUF7589	PF24500	785 - 919	2.2e-74	Domain of unknown function (DUF7589)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010595)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33820 FvH4_7g33820
malus_domestica	MD07G1306500.v1.1
prunus_persica	Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1
pyrus_communis	pycom07g27950
rosa_chinensis	RchiOBHm_Chr1g0383931
rosa_laevigata	RLG00000026085
rosa_multiflora	Rmu_sc0005139.1_g000026
rosa_roxburghii	Rroxscaffold_4G00277130
rosa_rugosa	Rorug01G0443100 Rorug01G0443200 Rorug01G0443400.1 Rorug01G0443500.1
rosa_samantha	Rh1AG469100 Rh1CG439600 Rh1DG459600
rosa_wichuraiana	Rw1G040330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1340, 2244
Acc16I	TGCGCA	1 cut(s)	494
Acc36I	ACCTGC	1 cut(s)	2032
AccB1I	GGYRCC	1 cut(s)	418
AccB7I	CCANNNNNTGG	2 cut(s)	2084, 2591
AccBSI	CCGCTC	1 cut(s)	229
AccI	GTMKAC	1 cut(s)	915
AccII	CGCG	1 cut(s)	746
AclWI	GGATC	7 cut(s)	332, 938, 1316, 1593, 1635, 1648, 1683
AcoI	YGGCCR	5 cut(s)	693, 747, 991, 1053, 2963
AcsI	RAATTY	4 cut(s)	317, 656, 1163, 1576
AcuI	CTGAAG	3 cut(s)	1490, 2469, 2907
AcvI	CACGTG	2 cut(s)	1134, 1328
AfaI	GTAC	5 cut(s)	173, 1695, 1744, 2151, 2645
AfiI	CCNNNNNNNGG	7 cut(s)	146, 288, 691, 1073, 2084, 2544, 2591
AflIII	ACRYGT	3 cut(s)	1131, 1133, 1327
AgsI	TTSAA	8 cut(s)	594, 1071, 1147, 1582, 2183, 2368, 2519, 3107
AhdI	GACNNNNNGTC	1 cut(s)	2027
AjiI	CACGTC	1 cut(s)	919
AjnI	CCWGG	4 cut(s)	889, 1202, 1873, 2584
AjuI	GAANNNNNNNTTGG	6 cut(s)	1149, 1181, 1664, 1696, 2646, 2678
AloI	GAACNNNNNNTCC	2 cut(s)	2367, 2399
Alw21I	GWGCWC	3 cut(s)	1559, 2064, 2606
Alw44I	GTGCAC	2 cut(s)	2060, 2602
AlwI	GGATC	7 cut(s)	332, 938, 1316, 1593, 1635, 1648, 1683
AlwNI	CAGNNNCTG	3 cut(s)	2137, 2498, 3014
AoxI	GGCC	8 cut(s)	224, 324, 693, 747, 869, 991, 1053, 2963
ApaLI	GTGCAC	2 cut(s)	2060, 2602
ApeKI	GCWGC	6 cut(s)	886, 1563, 2228, 2414, 2997, 3014
ApoI	RAATTY	4 cut(s)	317, 656, 1163, 1576
Asp700I	GAANNNNTTC	3 cut(s)	23, 165, 2509
AspLEI	GCGC	2 cut(s)	495, 782
AspS9I	GGNCC	4 cut(s)	673, 870, 2021, 2156
AsuC2I	CCSGG	1 cut(s)	835
AsuNHI	GCTAGC	1 cut(s)	539
AvaII	GGWCC	3 cut(s)	673, 2021, 2156
BaeGI	GKGCMC	3 cut(s)	421, 2064, 2606
BalI	TGGCCA	2 cut(s)	993, 1055
BamHI	GGATCC	1 cut(s)	1640
BanI	GGYRCC	1 cut(s)	418
BanII	GRGCYC	1 cut(s)	1559
BarI	GAAGNNNNNNTAC	6 cut(s)	1677, 1709, 2169, 2201, 2862, 2894
BauI	CACGAG	2 cut(s)	1274, 2204
BbrPI	CACGTG	2 cut(s)	1134, 1328
BbsI	GAAGAC	3 cut(s)	712, 1349, 2039
Bbv12I	GWGCWC	3 cut(s)	1559, 2064, 2606
BbvCI	CCTCAGC	1 cut(s)	710
BbvI	GCAGC	6 cut(s)	898, 1575, 2240, 2401, 3001, 3009
BccI	CCATC	9 cut(s)	104, 251, 803, 833, 1873, 2078, 2390, 2435, 3086
BceAI	ACGGC	5 cut(s)	680, 767, 803, 1673, 2447
BcgI	CGANNNNNNTGC	2 cut(s)	2309, 2343
BciT130I	CCWGG	4 cut(s)	891, 1204, 1875, 2586
BclI	TGATCA	1 cut(s)	1851
BcnI	CCSGG	1 cut(s)	835
BfmI	CTRYAG	4 cut(s)	1924, 2041, 2145, 2415
BfoI	RGCGCY	1 cut(s)	783
BfuAI	ACCTGC	1 cut(s)	2032
BglI	GCCNNNNNGGC	2 cut(s)	836, 980
BglII	AGATCT	1 cut(s)	1486
BmcAI	AGTACT	1 cut(s)	1744
Bme1390I	CCNGG	5 cut(s)	835, 891, 1204, 1875, 2586
Bme18I	GGWCC	3 cut(s)	673, 2021, 2156
BmeRI	GACNNNNNGTC	1 cut(s)	2027
BmgBI	CACGTC	1 cut(s)	919
BmgT120I	GGNCC	4 cut(s)	673, 870, 2021, 2156
BmiI	GGNNCC	4 cut(s)	420, 763, 1065, 1642
BmrFI	CCNGG	5 cut(s)	835, 891, 1204, 1875, 2586
BmtI	GCTAGC	1 cut(s)	543
BpiI	GAAGAC	3 cut(s)	712, 1349, 2039
BplI	GAGNNNNNCTC	2 cut(s)	1287, 1319
BpmI	CTGGAG	2 cut(s)	253, 1215
Bpu10I	CCTNAGC	2 cut(s)	710, 2849
BpuEI	CTTGAG	3 cut(s)	786, 1386, 1859
BpuMI	CCSGG	1 cut(s)	835
BsaAI	YACGTR	2 cut(s)	1134, 1328
BsaI	GGTCTC	2 cut(s)	1005, 1412
BsaJI	CCNNGG	9 cut(s)	282, 309, 744, 834, 988, 1422, 1874, 2814, 2938
BsaWI	WCCGGW	1 cut(s)	1007
BsaXI	ACNNNNNCTCC	4 cut(s)	903, 933, 1255, 1285
Bsc4I	CCNNNNNNNGG	7 cut(s)	146, 288, 691, 1073, 2084, 2544, 2591
Bse1I	ACTGG	6 cut(s)	928, 1485, 2240, 2257, 2381, 3048
Bse3DI	GCAATG	2 cut(s)	573, 1027
BseBI	CCWGG	4 cut(s)	891, 1204, 1875, 2586
BseDI	CCNNGG	9 cut(s)	282, 309, 744, 834, 988, 1422, 1874, 2814, 2938
BseLI	CCNNNNNNNGG	7 cut(s)	146, 288, 691, 1073, 2084, 2544, 2591
BseMI	GCAATG	2 cut(s)	573, 1027
BseMII	CTCAG	5 cut(s)	39, 649, 724, 1635, 2811
BseNI	ACTGG	6 cut(s)	928, 1485, 2240, 2257, 2381, 3048
BseRI	GAGGAG	6 cut(s)	589, 592, 691, 694, 1292, 1844
BseSI	GKGCMC	3 cut(s)	421, 2064, 2606
BseX3I	CGGCCG	1 cut(s)	747
BseXI	GCAGC	6 cut(s)	898, 1575, 2240, 2401, 3001, 3009
BseYI	CCCAGC	1 cut(s)	1103
BsgI	GTGCAG	1 cut(s)	2123
Bsh1236I	CGCG	1 cut(s)	746
Bsh1285I	CGRYCG	1 cut(s)	750
BshFI	GGCC	8 cut(s)	226, 326, 695, 749, 871, 993, 1055, 2965
BshNI	GGYRCC	1 cut(s)	418
BsiEI	CGRYCG	1 cut(s)	750
BsiHKAI	GWGCWC	3 cut(s)	1559, 2064, 2606
BsiSI	CCGG	5 cut(s)	759, 765, 834, 1008, 1391
BslFI	GGGAC	2 cut(s)	388, 475
BslI	CCNNNNNNNGG	7 cut(s)	146, 288, 691, 1073, 2084, 2544, 2591
BsmBI	CGTCTC	3 cut(s)	638, 703, 2628
BsmFI	GGGAC	2 cut(s)	388, 475
BsnI	GGCC	8 cut(s)	226, 326, 695, 749, 871, 993, 1055, 2965
Bso31I	GGTCTC	2 cut(s)	1005, 1412
Bsp1286I	GDGCHC	4 cut(s)	421, 1559, 2064, 2606
Bsp19I	CCATGG	2 cut(s)	988, 2814
BspANI	GGCC	8 cut(s)	226, 326, 695, 749, 871, 993, 1055, 2965
BspCNI	CTCAG	5 cut(s)	40, 648, 723, 1634, 2810
BspFNI	CGCG	1 cut(s)	746
BspLI	GGNNCC	4 cut(s)	420, 763, 1065, 1642
BspMAI	CTGCAG	2 cut(s)	2149, 2419
BspMI	ACCTGC	1 cut(s)	2032
BspOI	GCTAGC	1 cut(s)	543
BspPI	GGATC	7 cut(s)	332, 938, 1316, 1593, 1635, 1648, 1683
BspQI	GCTCTTC	2 cut(s)	29, 2865
BspT107I	GGYRCC	1 cut(s)	418
BspTNI	GGTCTC	2 cut(s)	1005, 1412
BsrBI	CCGCTC	1 cut(s)	229
BsrDI	GCAATG	2 cut(s)	573, 1027
BsrI	ACTGG	6 cut(s)	928, 1485, 2240, 2257, 2381, 3048
BssECI	CCNNGG	9 cut(s)	282, 309, 744, 834, 988, 1422, 1874, 2814, 2938
BssSI	CACGAG	2 cut(s)	1274, 2204
BssT1I	CCWWGG	6 cut(s)	282, 309, 988, 1422, 2814, 2938
Bst2BI	CACGAG	2 cut(s)	1274, 2204
Bst2UI	CCWGG	4 cut(s)	891, 1204, 1875, 2586
Bst6I	CTCTTC	2 cut(s)	29, 2865
BstAPI	GCANNNNNTGC	2 cut(s)	549, 3014
BstBAI	YACGTR	2 cut(s)	1134, 1328
BstC8I	GCNNGC	3 cut(s)	484, 541, 1347
BstDSI	CCRYGG	3 cut(s)	744, 988, 2814
BstENI	CCTNNNNNAGG	3 cut(s)	144, 286, 2542
BstFNI	CGCG	1 cut(s)	746
BstH2I	RGCGCY	1 cut(s)	783
BstHHI	GCGC	2 cut(s)	495, 782
BstMCI	CGRYCG	1 cut(s)	750
BstNI	CCWGG	4 cut(s)	891, 1204, 1875, 2586
BstNSI	RCATGY	1 cut(s)	552
BstSCI	CCNGG	5 cut(s)	833, 889, 1202, 1873, 2584
BstSFI	CTRYAG	4 cut(s)	1924, 2041, 2145, 2415
BstSLI	GKGCMC	3 cut(s)	421, 2064, 2606
BstUI	CGCG	1 cut(s)	746
BstV1I	GCAGC	6 cut(s)	898, 1575, 2240, 2401, 3001, 3009
BstV2I	GAAGAC	3 cut(s)	712, 1349, 2039
BstX2I	RGATCY	4 cut(s)	1486, 1598, 1640, 1675
BstXI	CCANNNNNNTGG	1 cut(s)	414
BstYI	RGATCY	4 cut(s)	1486, 1598, 1640, 1675
BstZI	CGGCCG	1 cut(s)	747
BsuRI	GGCC	8 cut(s)	226, 326, 695, 749, 871, 993, 1055, 2965
BtgI	CCRYGG	3 cut(s)	744, 988, 2814
BtgZI	GCGATG	2 cut(s)	811, 1185
BtrI	CACGTC	1 cut(s)	919
BtsI	GCAGTG	1 cut(s)	2142
BtsIMutI	CAGTG	8 cut(s)	45, 340, 361, 946, 2055, 2142, 2233, 2280
BveI	ACCTGC	1 cut(s)	2032
Cac8I	GCNNGC	3 cut(s)	484, 541, 1347
CaiI	CAGNNNCTG	3 cut(s)	2137, 2498, 3014
CciNI	GCGGCCGC	1 cut(s)	747
CfoI	GCGC	2 cut(s)	495, 782
Cfr13I	GGNCC	4 cut(s)	673, 870, 2021, 2156
Cfr42I	CCGCGG	1 cut(s)	747
CseI	GACGC	1 cut(s)	655
Csp6I	GTAC	5 cut(s)	172, 1694, 1743, 2150, 2644
CspCI	CAANNNNNGTGG	2 cut(s)	838, 873
CviQI	GTAC	5 cut(s)	172, 1694, 1743, 2150, 2644
DriI	GACNNNNNGTC	1 cut(s)	2027
EaeI	YGGCCR	5 cut(s)	693, 747, 991, 1053, 2963
EagI	CGGCCG	1 cut(s)	747
Eam1104I	CTCTTC	2 cut(s)	29, 2865
Eam1105I	GACNNNNNGTC	1 cut(s)	2027
EarI	CTCTTC	2 cut(s)	29, 2865
EciI	GGCGGA	1 cut(s)	595
Ecl136II	GAGCTC	1 cut(s)	1557
EclXI	CGGCCG	1 cut(s)	747
Eco130I	CCWWGG	6 cut(s)	282, 309, 988, 1422, 2814, 2938
Eco24I	GRGCYC	1 cut(s)	1559
Eco31I	GGTCTC	2 cut(s)	1005, 1412
Eco32I	GATATC	1 cut(s)	929
Eco47I	GGWCC	3 cut(s)	673, 2021, 2156
Eco52I	CGGCCG	1 cut(s)	747
Eco53kI	GAGCTC	1 cut(s)	1557
Eco57I	CTGAAG	3 cut(s)	1490, 2469, 2907
Eco72I	CACGTG	2 cut(s)	1134, 1328
EcoICRI	GAGCTC	1 cut(s)	1557
EcoNI	CCTNNNNNAGG	3 cut(s)	144, 286, 2542
EcoO109I	RGGNCCY	1 cut(s)	2156
EcoRII	CCWGG	4 cut(s)	889, 1202, 1873, 2584
EcoRV	GATATC	1 cut(s)	929
EcoT14I	CCWWGG	6 cut(s)	282, 309, 988, 1422, 2814, 2938
EcoT38I	GRGCYC	1 cut(s)	1559
ErhI	CCWWGG	6 cut(s)	282, 309, 988, 1422, 2814, 2938
Esp3I	CGTCTC	3 cut(s)	638, 703, 2628
FalI	AAGNNNNNCTT	2 cut(s)	1664, 1696
FaqI	GGGAC	2 cut(s)	388, 475
FbaI	TGATCA	1 cut(s)	1851
FblI	GTMKAC	1 cut(s)	915
FriOI	GRGCYC	1 cut(s)	1559
FspI	TGCGCA	1 cut(s)	494
GlaI	GCGC	2 cut(s)	494, 781
GsaI	CCCAGC	1 cut(s)	1107
GsuI	CTGGAG	2 cut(s)	253, 1215
HaeII	RGCGCY	1 cut(s)	783
HaeIII	GGCC	8 cut(s)	226, 326, 695, 749, 871, 993, 1055, 2965
HapII	CCGG	5 cut(s)	759, 765, 834, 1008, 1391
HgaI	GACGC	1 cut(s)	655
HhaI	GCGC	2 cut(s)	495, 782
Hin6I	GCGC	2 cut(s)	493, 780
HinP1I	GCGC	2 cut(s)	493, 780
HincII	GTYRAC	3 cut(s)	132, 277, 2988
HindII	GTYRAC	3 cut(s)	132, 277, 2988
HindIII	AAGCTT	3 cut(s)	9, 1343, 2481
HinfI	GANTC	7 cut(s)	278, 1047, 1108, 1296, 1765, 2288, 2717
HpaII	CCGG	5 cut(s)	759, 765, 834, 1008, 1391
Hpy188III	TCNNGA	7 cut(s)	232, 1274, 1449, 1748, 2051, 2159, 2633
Hpy99I	CGWCG	3 cut(s)	743, 824, 2925
HpyAV	CCTTC	9 cut(s)	43, 973, 1077, 1631, 1666, 2431, 2439, 2551, 3015
HpyCH4IV	ACGT	3 cut(s)	918, 1133, 1327
HpySE526I	ACGT	3 cut(s)	918, 1133, 1327
HspAI	GCGC	2 cut(s)	493, 780
Ksp22I	TGATCA	1 cut(s)	1851
KspI	CCGCGG	1 cut(s)	747
LguI	GCTCTTC	2 cut(s)	29, 2865
LmnI	GCTCC	5 cut(s)	395, 479, 719, 767, 1554
Lsp1109I	GCAGC	6 cut(s)	898, 1575, 2240, 2401, 3001, 3009
MaeII	ACGT	3 cut(s)	918, 1133, 1327
MaeIII	GTNAC	8 cut(s)	784, 811, 919, 1211, 1271, 1660, 1885, 2800
MbiI	CCGCTC	1 cut(s)	229
MfeI	CAATTG	1 cut(s)	2079
MflI	RGATCY	4 cut(s)	1486, 1598, 1640, 1675
MhlI	GDGCHC	4 cut(s)	421, 1559, 2064, 2606
MlsI	TGGCCA	2 cut(s)	993, 1055
MluNI	TGGCCA	2 cut(s)	993, 1055
MlyI	GAGTC	3 cut(s)	272, 1305, 1759
MmeI	TCCRAC	1 cut(s)	1975
Mox20I	TGGCCA	2 cut(s)	993, 1055
MroXI	GAANNNNTTC	3 cut(s)	23, 165, 2509
MscI	TGGCCA	2 cut(s)	993, 1055
MseI	TTAA	9 cut(s)	153, 246, 1208, 1521, 1607, 1904, 1982, 2793, 3054
MslI	CAYNNNNRTG	6 cut(s)	50, 365, 580, 1667, 2208, 2690
Msp20I	TGGCCA	2 cut(s)	993, 1055
MspA1I	CMGCKG	2 cut(s)	746, 3064
MspI	CCGG	5 cut(s)	759, 765, 834, 1008, 1391
MspR9I	CCNGG	5 cut(s)	835, 891, 1204, 1875, 2586
MunI	CAATTG	1 cut(s)	2079
MvaI	CCWGG	4 cut(s)	891, 1204, 1875, 2586
MvnI	CGCG	1 cut(s)	746
NciI	CCSGG	1 cut(s)	835
NcoI	CCATGG	2 cut(s)	988, 2814
NheI	GCTAGC	1 cut(s)	539
NlaIV	GGNNCC	4 cut(s)	420, 763, 1065, 1642
NmuCI	GTSAC	5 cut(s)	784, 811, 919, 1271, 1660
NotI	GCGGCCGC	1 cut(s)	747
NsbI	TGCGCA	1 cut(s)	494
NspI	RCATGY	1 cut(s)	552
PciSI	GCTCTTC	2 cut(s)	29, 2865
PdmI	GAANNNNTTC	3 cut(s)	23, 165, 2509
PfeI	GAWTC	4 cut(s)	1047, 1108, 2288, 2717
PflFI	GACNNNGTC	1 cut(s)	1269
PflMI	CCANNNNNTGG	2 cut(s)	2084, 2591
PleI	GAGTC	3 cut(s)	272, 1304, 1759
PmaCI	CACGTG	2 cut(s)	1134, 1328
PmlI	CACGTG	2 cut(s)	1134, 1328
PpsI	GAGTC	3 cut(s)	272, 1304, 1759
Ppu21I	YACGTR	2 cut(s)	1134, 1328
PpuMI	RGGWCCY	1 cut(s)	2156
PsiI	TTATAA	2 cut(s)	1340, 2244
Psp124BI	GAGCTC	1 cut(s)	1559
Psp5II	RGGWCCY	1 cut(s)	2156
Psp6I	CCWGG	4 cut(s)	889, 1202, 1873, 2584
PspCI	CACGTG	2 cut(s)	1134, 1328
PspFI	CCCAGC	1 cut(s)	1103
PspGI	CCWGG	4 cut(s)	889, 1202, 1873, 2584
PspN4I	GGNNCC	4 cut(s)	420, 763, 1065, 1642
PspPI	GGNCC	4 cut(s)	673, 870, 2021, 2156
PspPPI	RGGWCCY	1 cut(s)	2156
PstI	CTGCAG	2 cut(s)	2149, 2419
PstNI	CAGNNNCTG	3 cut(s)	2137, 2498, 3014
PsuI	RGATCY	4 cut(s)	1486, 1598, 1640, 1675
PsyI	GACNNNGTC	1 cut(s)	1269
PvuII	CAGCTG	1 cut(s)	3064
RsaI	GTAC	5 cut(s)	173, 1695, 1744, 2151, 2645
RsaNI	GTAC	5 cut(s)	172, 1694, 1743, 2150, 2644
RseI	CAYNNNNRTG	6 cut(s)	50, 365, 580, 1667, 2208, 2690
SacI	GAGCTC	1 cut(s)	1559
SacII	CCGCGG	1 cut(s)	747
SapI	GCTCTTC	2 cut(s)	29, 2865
SaqAI	TTAA	9 cut(s)	153, 246, 1208, 1521, 1607, 1904, 1982, 2793, 3054
Sau96I	GGNCC	4 cut(s)	673, 870, 2021, 2156
ScaI	AGTACT	1 cut(s)	1744
SchI	GAGTC	3 cut(s)	272, 1305, 1759
ScrFI	CCNGG	5 cut(s)	835, 891, 1204, 1875, 2586
SduI	GDGCHC	4 cut(s)	421, 1559, 2064, 2606
SfcI	CTRYAG	4 cut(s)	1924, 2041, 2145, 2415
Sfr303I	CCGCGG	1 cut(s)	747
SgrBI	CCGCGG	1 cut(s)	747
SinI	GGWCC	3 cut(s)	673, 2021, 2156
SmiMI	CAYNNNNRTG	6 cut(s)	50, 365, 580, 1667, 2208, 2690
SmlI	CTYRAG	3 cut(s)	801, 1401, 1838
SmoI	CTYRAG	3 cut(s)	801, 1401, 1838
SspI	AATATT	2 cut(s)	2510, 2726
SstI	GAGCTC	1 cut(s)	1559
StyD4I	CCNGG	5 cut(s)	833, 889, 1202, 1873, 2584
StyI	CCWWGG	6 cut(s)	282, 309, 988, 1422, 2814, 2938
TaiI	ACGT	3 cut(s)	921, 1136, 1330
TaqI	TCGA	7 cut(s)	27, 439, 879, 1701, 2052, 2632, 2920
TatI	WGTACW	3 cut(s)	171, 1742, 2149
TauI	GCSGC	9 cut(s)	83, 229, 746, 749, 752, 755, 1902, 2968, 3008
TfiI	GAWTC	4 cut(s)	1047, 1108, 2288, 2717
Tru1I	TTAA	9 cut(s)	153, 246, 1208, 1521, 1607, 1904, 1982, 2793, 3054
Tru9I	TTAA	9 cut(s)	153, 246, 1208, 1521, 1607, 1904, 1982, 2793, 3054
TscAI	CASTG	8 cut(s)	52, 340, 361, 946, 2062, 2149, 2240, 2287
TseFI	GTSAC	5 cut(s)	784, 811, 919, 1271, 1660
TseI	GCWGC	6 cut(s)	886, 1563, 2228, 2414, 2997, 3014
Tsp45I	GTSAC	5 cut(s)	784, 811, 919, 1271, 1660
TspGWI	ACGGA	4 cut(s)	269, 330, 706, 3051
TspRI	CASTG	8 cut(s)	52, 340, 361, 946, 2062, 2149, 2240, 2287
Tth111I	GACNNNGTC	1 cut(s)	1269
Van91I	CCANNNNNTGG	2 cut(s)	2084, 2591
VneI	GTGCAC	2 cut(s)	2060, 2602
VpaK11BI	GGWCC	3 cut(s)	673, 2021, 2156
XagI	CCTNNNNNAGG	3 cut(s)	144, 286, 2542
XapI	RAATTY	4 cut(s)	317, 656, 1163, 1576
XbaI	TCTAGA	1 cut(s)	1747
XceI	RCATGY	1 cut(s)	552
XmiI	GTMKAC	1 cut(s)	915
XmnI	GAANNNNTTC	3 cut(s)	23, 165, 2509
ZrmI	AGTACT	1 cut(s)	1744

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.