Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0010595

Physical Location & Seq

Genomic Coordinates Reverse (-)

27470554 .. 27476000

Gene Structure 5447 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g27950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGACATGCCAAGGCTCCTCCACCACCACCACCACCATGTTAGCCCACAAATTCCACCGCCTCCACCTCCGCAATTCCCCTCAGTTCCGATGTGTTCTGGACCAAATCGCCCCCAATCTGGCCGTCTCGGCGTCCCTCAGTTCCGTCTTCACCTCTGCCAACGTCATCGCCACTGCCTCCGGCACTAGCTCTCTCCACGGTGCCGTCACCTCCACCATCACTCAAGTCGCGGTCACCGCCGTCGCCATCGCCTCCGGGGCTTGTCTTTCCACCAAGGTCGATTTCTTGTGGCCCAAAATGGAAGCCCAGCCAGGTTCTGATGTGGTGGAGGGAGTGGACGTGACTGGATATCCTATCTTCAATGATCCAAAGGTGCAAAAGGCTGTTGCATTTGCTAAGAAAGCTCACCATGGCCAACTGCGCAAAACCGGAGACCCTTATTTAGTCCATTGCATTCATACAGGAAGAATCTTGGCCATGCTGGTTCCTGCAAGCGGAGAGCGAGCTGTTGAGACTGTTGTAGCCGGGATTCTCCATGATGTGGTGGACGATACATGTGTAAGTTTCTCCGATATACAACAAGAGTTTGGTGATCATGTTGCGAAGCTGGTTGCTGGTGTTTCCAGGTTAAGCTACATAAATCAGCTTTTACGGAGACGCCGCAGGATAAATGTGAACCAGGGAAAACTTGGTCATGAGGAGGCAAATAATTTACGAGTTATGCTTCTGGGCATGGTTGATGATCCACGTGTTGTGCTTATAAAGCTTGCAGATCGTCTTCACAATATGAGAACAATTTATGCTCTCCCGCTGCCAAAGGCTCAAGCTGTTGCAAGGGAGACCTTGGTAATTTGGTGCTCGCTGGCTTCCAGATTGGGTTTGTGGGCAATGAAAGCTGAACTGGAAGATCTGTGCTTTGCTGTTCTTCAGCCTGAGATGTTCAAGAAAATGAGAGCTGATCTAGCTTCTATGTGGAGCTCTAGTAACAAAGTAGGAAATTCAAAAAGAAAATCCAGTTTGCTGTCCTTAAATGGAAGAAGCTCAATTTCCGACAACGAGGGGCCTGTAGCAGTTGATGAAGATGTCACAACCATGAAGGATCTTTTGGAAGCTGTAGTGCCATTTGATGTATTGTTAGATCGAAGAAAACGGTCTAAATTTCTTAATGCTCTAGGACAAGGTTTGGAGACTCACAGAAGAGCAAAGGTTGTGCAAGATGCTGGAATTGCTTTGGCATCTATGGTCATTTGTGAGGAGGCCCTCGAGCAGGAGTTTATCATATCAACTTCTTATGTTCCAGGGATGGAAGTAACTTTATCGAGCCGCTTGAAAAGTTTGTATAGCATCTACAGCAAGATGAAACGGAAAGATGTCAGCATTAGTAAAGTTTATGATGCCCGTGCATTAAGGGTAGTCGTTGGAGACAAGAACGGAACTTTGCATGGGCCTGCTGTCCAGTGTTGTTACAGCCTTCTTGACATTGTACACAAGCATTGGACCCCAATTGATGGTGAGTTTGATGATTACATTGTCAATCCAAAGCCTAGTGGCTATCAGTCTCTGCACACTGCAGTACAAGGTCCTGACAGATCACCTTTGGAAGTTCAAATACGAACACAGAGGATGCACGAGTACGCTGAACATGGACTTGCAGCACATTGGCTTTATAAAGAGACTGGAAATACGTTTTCTAACATAAGTAGCACGGATGAATCTGAAATAGACGTATCAGCCTTTTTATCCAAAGATATTGAGGACCAAAGTTCTACAGAAGAAGATTTTTCTCAAAAGTATGATTTGTTGAAAATTGGACATCCAGTCCTTAGAGTGGAAGGGAGTCATCTTCTTGCCGCTGTTATCATTAGAGTAGAGAAAGATGGAAGGGAGTTGATAGTTGCTGTGAGCTTTGGACTAGCTGCTTCTGAAGCAGTAGCTGATAGAAAATCTTCTTTCCAAATAAAACGGTGGGAAGCCTATGCAAGACTATACAAGAAGGTGACGGATGAATGGTGGTGTGAACCAGGACATGGGGATTGGTGTACCTGCCTAGAGAGATATACACTCTGTCGAGATGGAATGTACCACAAGCAAGACCAGTTTGGGCGATTACTTCCAACCTTCATTCAGGTCATTGACTTGACAGACCAAGAAGAATCGGAATATTGGTCTGTTGTTTCTGCTGTTTTTGACGGAAAAGAGCTCGACGATGTTACATCCACCGCAAGCTTTAGCTCAGTTCCGTCAACTTCCATGGAAACTAGCATCAACAACAAGGTACGTTTGTTGAGGACGATGCTGCGATGGGAAGAACAGCTACGCTCAGAAGCAAGTCTTGGACAAGCAAAGCATAGCAGCAAGTTTTACCGCAGTCCAGGTTCTGTTGTCCTTGGAGAAGTGGTGATTATATGTTGGCCCGATGGTGAAATAATGCGGTTGAGAACCGGCAGCACCGCAGCAGATGCCGCAAGAAGAGTAGGACTGGAGGGAAAACTGGTCCTGGTTAACGGTCAGCTGGTTTTACCTAATACAAAGCTCACAGATGGCGACGTCGTTGAAGTCAGAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009259 GO:0009605 GO:0009987 GO:0009991 GO:0015969 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0033865 GO:0033875 GO:0034032 GO:0034035 GO:0034641 GO:0042278 GO:0042578 GO:0042594 GO:0044237 GO:0044238 GO:0044281 GO:0044464 GO:0046128 GO:0046483 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:1901068 GO:1901135 GO:1901360 GO:1901564 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00230 map00230

Pfam Domains

Protein Families

HD_4 RelA_SpoT TGS

Protein Analysis

856

Amino Acids

94.6

Weight (kDa)

6.7

Isoelectric Point (pI)

39.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HD_4	PF13328	128 - 295	2.8e-43	HD domain
HD	PF01966	148 - 263	1.4e-07	HD domain
RelA_SpoT	PF04607	441 - 560	6.9e-36	Region found in RelA / SpoT proteins
DUF7589	PF24500	596 - 731	1.3e-75	Domain of unknown function (DUF7589)
TGS	PF02824	801 - 853	1.3e-07	TGS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010595)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33820 FvH4_7g33820
malus_domestica	MD07G1306500.v1.1
prunus_persica	Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1 Prupe.2G324500_v2.0.a1
pyrus_communis	pycom07g27950
rosa_chinensis	RchiOBHm_Chr1g0383931
rosa_laevigata	RLG00000026085
rosa_multiflora	Rmu_sc0005139.1_g000026
rosa_roxburghii	Rroxscaffold_4G00277130
rosa_rugosa	Rorug01G0443100 Rorug01G0443200 Rorug01G0443400.1 Rorug01G0443500.1
rosa_samantha	Rh1AG469100 Rh1CG439600 Rh1DG459600
rosa_wichuraiana	Rw1G040330

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	761, 1668
AatII	GACGTC	1 cut(s)	2550
Acc16I	TGCGCA	1 cut(s)	422
Acc36I	ACCTGC	1 cut(s)	2051
AccB1I	GGYRCC	1 cut(s)	200
AccB7I	CCANNNNNTGG	3 cut(s)	541, 1508, 2027
AccII	CGCG	1 cut(s)	230
AclWI	GGATC	3 cut(s)	359, 737, 1107
AcoI	YGGCCR	3 cut(s)	120, 412, 474
AcsI	RAATTY	3 cut(s)	50, 997, 1157
AcuI	CTGAAG	2 cut(s)	911, 1944
AcvI	CACGTG	1 cut(s)	749
AcyI	GRCGYC	3 cut(s)	131, 658, 2547
AfaI	GTAC	6 cut(s)	1485, 1575, 1634, 2041, 2081, 2277
AfiI	CCNNNNNNNGG	7 cut(s)	118, 494, 541, 1267, 1508, 1828, 2027
AflIII	ACRYGT	2 cut(s)	554, 748
AgsI	TTSAA	7 cut(s)	361, 943, 1002, 1330, 1607, 1804, 2555
AhdI	GACNNNNNGTC	1 cut(s)	1817
AjiI	CACGTC	1 cut(s)	340
AjnI	CCWGG	7 cut(s)	310, 623, 678, 1297, 2020, 2371, 2496
AjuI	GAANNNNNNNTTGG	6 cut(s)	266, 298, 1088, 1120, 2316, 2348
Alw21I	GWGCWC	3 cut(s)	860, 980, 2202
Alw26I	GTCTC	9 cut(s)	130, 426, 506, 649, 833, 1181, 1416, 1563, 1667
AlwI	GGATC	3 cut(s)	359, 737, 1107
AlwNI	CAGNNNCTG	4 cut(s)	317, 1561, 1934, 2378
Ama87I	CYCGRG	1 cut(s)	1262
AoxI	GGCC	8 cut(s)	120, 290, 412, 474, 1061, 1257, 1445, 2411
ApeKI	GCWGC	7 cut(s)	811, 1652, 1916, 2296, 2352, 2445, 2453
ApoI	RAATTY	3 cut(s)	50, 997, 1157
Asp700I	GAANNNNTTC	1 cut(s)	1945
AspLEI	GCGC	1 cut(s)	423
AsuC2I	CCSGG	2 cut(s)	256, 526
AvaI	CYCGRG	1 cut(s)	1262
AvaII	GGWCC	5 cut(s)	100, 1497, 1580, 1756, 2494
BaeI	ACNNNNGTAYC	4 cut(s)	543, 576, 2267, 2300
BalI	TGGCCA	2 cut(s)	414, 476
BanI	GGYRCC	1 cut(s)	200
BanII	GRGCYC	2 cut(s)	980, 2202
BarI	GAAGNNNNNNTAC	4 cut(s)	1593, 1625, 2298, 2330
BauI	CACGAG	1 cut(s)	1628
BbrPI	CACGTG	1 cut(s)	749
BbsI	GAAGAC	2 cut(s)	139, 770
Bbv12I	GWGCWC	3 cut(s)	860, 980, 2202
BbvI	GCAGC	7 cut(s)	798, 1664, 1903, 2283, 2364, 2457, 2465
BccI	CCATC	9 cut(s)	224, 254, 1297, 1502, 1871, 2066, 2295, 2411, 2534
BceAI	ACGGC	3 cut(s)	107, 188, 224
BciT130I	CCWGG	7 cut(s)	312, 625, 680, 1299, 2022, 2373, 2498
BclI	TGATCA	1 cut(s)	592
BcnI	CCSGG	2 cut(s)	256, 526
BcoDI	GTCTC	9 cut(s)	130, 426, 506, 649, 833, 1181, 1416, 1563, 1667
BfaI	CTAG	8 cut(s)	186, 962, 981, 1172, 1545, 1913, 2048, 2259
BfmI	CTRYAG	5 cut(s)	1065, 1113, 1348, 1569, 1767
BfuAI	ACCTGC	1 cut(s)	2051
BglI	GCCNNNNNGGC	2 cut(s)	128, 257
BglII	AGATCT	1 cut(s)	907
Bme1390I	CCNGG	9 cut(s)	256, 312, 526, 625, 680, 1299, 2022, 2373, 2498
Bme18I	GGWCC	5 cut(s)	100, 1497, 1580, 1756, 2494
BmeRI	GACNNNNNGTC	1 cut(s)	1817
BmeT110I	CYCGRG	1 cut(s)	1262
BmgBI	CACGTC	1 cut(s)	340
BmiI	GGNNCC	5 cut(s)	16, 202, 486, 1062, 1499
BmrFI	CCNGG	9 cut(s)	256, 312, 526, 625, 680, 1299, 2022, 2373, 2498
BmsI	GCATC	8 cut(s)	1207, 1244, 1353, 1384, 1614, 2271, 2283, 2449
BpiI	GAAGAC	2 cut(s)	139, 770
BpmI	CTGGAG	1 cut(s)	2501
BpuEI	CTTGAG	2 cut(s)	207, 807
BpuMI	CCSGG	2 cut(s)	256, 526
BsaAI	YACGTR	1 cut(s)	749
BsaHI	GRCGYC	3 cut(s)	131, 658, 2547
BsaI	GGTCTC	2 cut(s)	426, 833
BsaWI	WCCGGW	1 cut(s)	428
BsaXI	ACNNNNNCTCC	6 cut(s)	324, 354, 967, 997, 2382, 2412
Bsc4I	CCNNNNNNNGG	7 cut(s)	118, 494, 541, 1267, 1508, 1828, 2027
Bse118I	RCCGGY	1 cut(s)	2441
Bse1I	ACTGG	9 cut(s)	349, 906, 1014, 1456, 1681, 1817, 2095, 2484, 2496
Bse3DI	GCAATG	2 cut(s)	448, 894
BseBI	CCWGG	7 cut(s)	312, 625, 680, 1299, 2022, 2373, 2498
BseGI	GGATG	6 cut(s)	1308, 1629, 1714, 1813, 2008, 2213
BseLI	CCNNNNNNNGG	7 cut(s)	118, 494, 541, 1267, 1508, 1828, 2027
BseMI	GCAATG	2 cut(s)	448, 894
BseMII	CTCAG	5 cut(s)	95, 151, 924, 2247, 2334
BseNI	ACTGG	9 cut(s)	349, 906, 1014, 1456, 1681, 1817, 2095, 2484, 2496
BseRI	GAGGAG	3 cut(s)	7, 713, 1268
BseXI	GCAGC	7 cut(s)	798, 1664, 1903, 2283, 2364, 2457, 2465
BseYI	CCCAGC	1 cut(s)	306
BsgI	GTGCAG	1 cut(s)	1547
Bsh1236I	CGCG	1 cut(s)	230
BshFI	GGCC	8 cut(s)	122, 292, 414, 476, 1063, 1259, 1447, 2413
BshNI	GGYRCC	1 cut(s)	200
BsiHKAI	GWGCWC	3 cut(s)	860, 980, 2202
BsiHKCI	CYCGRG	1 cut(s)	1262
BsiSI	CCGG	5 cut(s)	180, 255, 429, 525, 2442
BslFI	GGGAC	1 cut(s)	118
BslI	CCNNNNNNNGG	7 cut(s)	118, 494, 541, 1267, 1508, 1828, 2027
BsmAI	GTCTC	9 cut(s)	130, 426, 506, 649, 833, 1181, 1416, 1563, 1667
BsmBI	CGTCTC	2 cut(s)	130, 649
BsmFI	GGGAC	1 cut(s)	118
BsmI	GAATGC	1 cut(s)	453
BsnI	GGCC	8 cut(s)	122, 292, 414, 476, 1063, 1259, 1447, 2413
Bso31I	GGTCTC	2 cut(s)	426, 833
BsoBI	CYCGRG	1 cut(s)	1262
Bsp1286I	GDGCHC	3 cut(s)	860, 980, 2202
Bsp1407I	TGTACA	1 cut(s)	1483
Bsp143I	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
Bsp19I	CCATGG	2 cut(s)	409, 2250
BspANI	GGCC	8 cut(s)	122, 292, 414, 476, 1063, 1259, 1447, 2413
BspCNI	CTCAG	5 cut(s)	94, 150, 925, 2246, 2333
BspFNI	CGCG	1 cut(s)	230
BspHI	TCATGA	1 cut(s)	694
BspLI	GGNNCC	5 cut(s)	16, 202, 486, 1062, 1499
BspMAI	CTGCAG	1 cut(s)	1573
BspMI	ACCTGC	1 cut(s)	2051
BspPI	GGATC	3 cut(s)	359, 737, 1107
BspQI	GCTCTTC	1 cut(s)	1192
BspT107I	GGYRCC	1 cut(s)	200
BspTNI	GGTCTC	2 cut(s)	426, 833
BsrDI	GCAATG	2 cut(s)	448, 894
BsrFI	RCCGGY	1 cut(s)	2441
BsrGI	TGTACA	1 cut(s)	1483
BsrI	ACTGG	9 cut(s)	349, 906, 1014, 1456, 1681, 1817, 2095, 2484, 2496
BssAI	RCCGGY	1 cut(s)	2441
BssMI	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
BssNI	GRCGYC	3 cut(s)	131, 658, 2547
BssSI	CACGAG	1 cut(s)	1628
BssT1I	CCWWGG	6 cut(s)	10, 273, 409, 843, 2250, 2386
Bst2BI	CACGAG	1 cut(s)	1628
Bst2UI	CCWGG	7 cut(s)	312, 625, 680, 1299, 2022, 2373, 2498
Bst4CI	ACNGT	5 cut(s)	200, 517, 1152, 1965, 2507
Bst6I	CTCTTC	2 cut(s)	1192, 2464
BstACI	GRCGYC	3 cut(s)	131, 658, 2547
BstAPI	GCANNNNNTGC	1 cut(s)	2459
BstAUI	TGTACA	1 cut(s)	1483
BstBAI	YACGTR	1 cut(s)	749
BstC8I	GCNNGC	7 cut(s)	493, 504, 768, 860, 864, 1449, 2224
BstDEI	CTNAG	7 cut(s)	81, 137, 396, 933, 1823, 2233, 2320
BstDSI	CCRYGG	3 cut(s)	196, 409, 2250
BstEII	GGTNACC	1 cut(s)	232
BstENI	CCTNNNNNAGG	1 cut(s)	1265
BstF5I	GGATG	6 cut(s)	1308, 1629, 1714, 1813, 2008, 2213
BstFNI	CGCG	1 cut(s)	230
BstHHI	GCGC	1 cut(s)	423
BstKTI	GATC	9 cut(s)	367, 595, 745, 775, 910, 961, 1102, 1141, 1592
BstMAI	GTCTC	9 cut(s)	130, 426, 506, 649, 833, 1181, 1416, 1563, 1667
BstMBI	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
BstNI	CCWGG	7 cut(s)	312, 625, 680, 1299, 2022, 2373, 2498
BstNSI	RCATGY	2 cut(s)	9, 558
BstPI	GGTNACC	1 cut(s)	232
BstSCI	CCNGG	9 cut(s)	254, 310, 524, 623, 678, 1297, 2020, 2371, 2496
BstSFI	CTRYAG	5 cut(s)	1065, 1113, 1348, 1569, 1767
BstUI	CGCG	1 cut(s)	230
BstV1I	GCAGC	7 cut(s)	798, 1664, 1903, 2283, 2364, 2457, 2465
BstV2I	GAAGAC	2 cut(s)	139, 770
BstX2I	RGATCY	2 cut(s)	907, 1099
BstYI	RGATCY	2 cut(s)	907, 1099
BsuRI	GGCC	8 cut(s)	122, 292, 414, 476, 1063, 1259, 1447, 2413
BtgI	CCRYGG	3 cut(s)	196, 409, 2250
BtgZI	GCGATG	3 cut(s)	151, 232, 2314
BtrI	CACGTC	1 cut(s)	340
BtsCI	GGATG	6 cut(s)	1308, 1629, 1714, 1813, 2008, 2213
BtsI	GCAGTG	2 cut(s)	171, 1566
BtsIMutI	CAGTG	3 cut(s)	171, 1463, 1566
BveI	ACCTGC	1 cut(s)	2051
Cac8I	GCNNGC	7 cut(s)	493, 504, 768, 860, 864, 1449, 2224
CaiI	CAGNNNCTG	4 cut(s)	317, 1561, 1934, 2378
CciI	TCATGA	1 cut(s)	694
CfoI	GCGC	1 cut(s)	423
Cfr10I	RCCGGY	1 cut(s)	2441
CseI	GACGC	2 cut(s)	120, 666
Csp6I	GTAC	6 cut(s)	1484, 1574, 1633, 2040, 2080, 2276
CviQI	GTAC	6 cut(s)	1484, 1574, 1633, 2040, 2080, 2276
DdeI	CTNAG	7 cut(s)	81, 137, 396, 933, 1823, 2233, 2320
DpnI	GATC	9 cut(s)	366, 594, 744, 774, 909, 960, 1101, 1140, 1591
DpnII	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
DriI	GACNNNNNGTC	1 cut(s)	1817
EaeI	YGGCCR	3 cut(s)	120, 412, 474
Eam1104I	CTCTTC	2 cut(s)	1192, 2464
Eam1105I	GACNNNNNGTC	1 cut(s)	1817
EarI	CTCTTC	2 cut(s)	1192, 2464
Ecl136II	GAGCTC	2 cut(s)	978, 2200
Eco130I	CCWWGG	6 cut(s)	10, 273, 409, 843, 2250, 2386
Eco24I	GRGCYC	2 cut(s)	980, 2202
Eco31I	GGTCTC	2 cut(s)	426, 833
Eco32I	GATATC	1 cut(s)	350
Eco47I	GGWCC	5 cut(s)	100, 1497, 1580, 1756, 2494
Eco53kI	GAGCTC	2 cut(s)	978, 2200
Eco57I	CTGAAG	2 cut(s)	911, 1944
Eco72I	CACGTG	1 cut(s)	749
Eco88I	CYCGRG	1 cut(s)	1262
Eco91I	GGTNACC	1 cut(s)	232
EcoICRI	GAGCTC	2 cut(s)	978, 2200
EcoNI	CCTNNNNNAGG	1 cut(s)	1265
EcoO109I	RGGNCCY	3 cut(s)	1061, 1258, 1580
EcoO65I	GGTNACC	1 cut(s)	232
EcoRII	CCWGG	7 cut(s)	310, 623, 678, 1297, 2020, 2371, 2496
EcoRV	GATATC	1 cut(s)	350
EcoT14I	CCWWGG	6 cut(s)	10, 273, 409, 843, 2250, 2386
EcoT38I	GRGCYC	2 cut(s)	980, 2202
ErhI	CCWWGG	6 cut(s)	10, 273, 409, 843, 2250, 2386
Esp3I	CGTCTC	2 cut(s)	130, 649
FalI	AAGNNNNNCTT	2 cut(s)	2316, 2348
FaqI	GGGAC	1 cut(s)	118
FauI	CCCGC	1 cut(s)	816
FbaI	TGATCA	1 cut(s)	592
FokI	GGATG	6 cut(s)	1315, 1636, 1721, 1800, 2015, 2200
FriOI	GRGCYC	2 cut(s)	980, 2202
FspBI	CTAG	8 cut(s)	186, 962, 981, 1172, 1545, 1913, 2048, 2259
FspI	TGCGCA	1 cut(s)	422
GlaI	GCGC	1 cut(s)	422
GsaI	CCCAGC	1 cut(s)	310
GsuI	CTGGAG	1 cut(s)	2501
HaeIII	GGCC	8 cut(s)	122, 292, 414, 476, 1063, 1259, 1447, 2413
HapII	CCGG	5 cut(s)	180, 255, 429, 525, 2442
HgaI	GACGC	2 cut(s)	120, 666
HhaI	GCGC	1 cut(s)	423
Hin1I	GRCGYC	3 cut(s)	131, 658, 2547
Hin6I	GCGC	1 cut(s)	421
HinP1I	GCGC	1 cut(s)	421
HincII	GTYRAC	2 cut(s)	2244, 2503
HindII	GTYRAC	2 cut(s)	2244, 2503
HindIII	AAGCTT	2 cut(s)	764, 2224
HinfI	GANTC	6 cut(s)	468, 529, 1189, 1712, 1837, 2153
HpaI	GTTAAC	1 cut(s)	2503
HpaII	CCGG	5 cut(s)	180, 255, 429, 525, 2442
Hpy166II	GTNNAC	8 cut(s)	337, 547, 676, 1486, 2018, 2040, 2244, 2503
Hpy188I	TCNGA	9 cut(s)	89, 319, 571, 1051, 1717, 1924, 2158, 2323, 2561
Hpy188III	TCNNGA	7 cut(s)	98, 695, 870, 943, 1475, 1583, 2069
Hpy8I	GTNNAC	8 cut(s)	337, 547, 676, 1486, 2018, 2040, 2244, 2503
Hpy99I	CGWCG	4 cut(s)	245, 2207, 2549, 2552
HpyAV	CCTTC	6 cut(s)	1090, 1481, 1826, 1875, 1987, 2128
HpyCH4III	ACNGT	5 cut(s)	200, 517, 1152, 1965, 2507
HpyCH4IV	ACGT	7 cut(s)	162, 339, 748, 1685, 1725, 2278, 2547
HpyF3I	CTNAG	7 cut(s)	81, 137, 396, 933, 1823, 2233, 2320
HpySE526I	ACGT	7 cut(s)	162, 339, 748, 1685, 1725, 2278, 2547
Hsp92I	GRCGYC	3 cut(s)	131, 658, 2547
HspAI	GCGC	1 cut(s)	421
Ksp22I	TGATCA	1 cut(s)	592
KspAI	GTTAAC	1 cut(s)	2503
Kzo9I	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
LguI	GCTCTTC	1 cut(s)	1192
LmnI	GCTCC	2 cut(s)	20, 975
Lsp1109I	GCAGC	7 cut(s)	798, 1664, 1903, 2283, 2364, 2457, 2465
LweI	GCATC	8 cut(s)	1207, 1244, 1353, 1384, 1614, 2271, 2283, 2449
MaeI	CTAG	8 cut(s)	186, 962, 981, 1172, 1545, 1913, 2048, 2259
MaeII	ACGT	7 cut(s)	162, 339, 748, 1685, 1725, 2278, 2547
MaeIII	GTNAC	9 cut(s)	205, 232, 340, 983, 1084, 1309, 1463, 1996, 2209
MalI	GATC	9 cut(s)	366, 594, 744, 774, 909, 960, 1101, 1140, 1591
MboI	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
MfeI	CAATTG	1 cut(s)	1503
MflI	RGATCY	2 cut(s)	907, 1099
MhlI	GDGCHC	3 cut(s)	860, 980, 2202
MlsI	TGGCCA	2 cut(s)	414, 476
MluNI	TGGCCA	2 cut(s)	414, 476
MlyI	GAGTC	2 cut(s)	1183, 1846
MmeI	TCCRAC	3 cut(s)	1074, 1399, 2138
Mox20I	TGGCCA	2 cut(s)	414, 476
MroXI	GAANNNNTTC	1 cut(s)	1945
MscI	TGGCCA	2 cut(s)	414, 476
MseI	TTAA	5 cut(s)	629, 1028, 1164, 1406, 2502
MslI	CAYNNNNRTG	2 cut(s)	35, 1091
Msp20I	TGGCCA	2 cut(s)	414, 476
MspA1I	CMGCKG	3 cut(s)	811, 1853, 2512
MspI	CCGG	5 cut(s)	180, 255, 429, 525, 2442
MspR9I	CCNGG	9 cut(s)	256, 312, 526, 625, 680, 1299, 2022, 2373, 2498
MunI	CAATTG	1 cut(s)	1503
Mva1269I	GAATGC	1 cut(s)	453
MvaI	CCWGG	7 cut(s)	312, 625, 680, 1299, 2022, 2373, 2498
MvnI	CGCG	1 cut(s)	230
NciI	CCSGG	2 cut(s)	256, 526
NcoI	CCATGG	2 cut(s)	409, 2250
NdeII	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
NlaIV	GGNNCC	5 cut(s)	16, 202, 486, 1062, 1499
NmeAIII	GCCGAG	1 cut(s)	107
NmuCI	GTSAC	5 cut(s)	205, 232, 340, 1084, 1996
NsbI	TGCGCA	1 cut(s)	422
NspI	RCATGY	2 cut(s)	9, 558
PaeR7I	CTCGAG	1 cut(s)	1262
PagI	TCATGA	1 cut(s)	694
PciI	ACATGT	1 cut(s)	554
PciSI	GCTCTTC	1 cut(s)	1192
PctI	GAATGC	1 cut(s)	453
PdmI	GAANNNNTTC	1 cut(s)	1945
PfeI	GAWTC	4 cut(s)	468, 529, 1712, 2153
PflMI	CCANNNNNTGG	3 cut(s)	541, 1508, 2027
PleI	GAGTC	2 cut(s)	1183, 1845
PmaCI	CACGTG	1 cut(s)	749
PmlI	CACGTG	1 cut(s)	749
PpsI	GAGTC	2 cut(s)	1183, 1845
Ppu21I	YACGTR	1 cut(s)	749
PpuMI	RGGWCCY	1 cut(s)	1580
PscI	ACATGT	1 cut(s)	554
PsiI	TTATAA	2 cut(s)	761, 1668
Psp124BI	GAGCTC	2 cut(s)	980, 2202
Psp5II	RGGWCCY	1 cut(s)	1580
Psp6I	CCWGG	7 cut(s)	310, 623, 678, 1297, 2020, 2371, 2496
PspCI	CACGTG	1 cut(s)	749
PspEI	GGTNACC	1 cut(s)	232
PspFI	CCCAGC	1 cut(s)	306
PspGI	CCWGG	7 cut(s)	310, 623, 678, 1297, 2020, 2371, 2496
PspN4I	GGNNCC	5 cut(s)	16, 202, 486, 1062, 1499
PspPPI	RGGWCCY	1 cut(s)	1580
PspXI	VCTCGAGB	1 cut(s)	1262
PstI	CTGCAG	1 cut(s)	1573
PstNI	CAGNNNCTG	4 cut(s)	317, 1561, 1934, 2378
PsuI	RGATCY	2 cut(s)	907, 1099
PvuII	CAGCTG	1 cut(s)	2512
RsaI	GTAC	6 cut(s)	1485, 1575, 1634, 2041, 2081, 2277
RsaNI	GTAC	6 cut(s)	1484, 1574, 1633, 2040, 2080, 2276
RseI	CAYNNNNRTG	2 cut(s)	35, 1091
SacI	GAGCTC	2 cut(s)	980, 2202
SapI	GCTCTTC	1 cut(s)	1192
SaqAI	TTAA	5 cut(s)	629, 1028, 1164, 1406, 2502
Sau3AI	GATC	9 cut(s)	364, 592, 742, 772, 907, 958, 1099, 1138, 1589
SchI	GAGTC	2 cut(s)	1183, 1846
ScrFI	CCNGG	9 cut(s)	256, 312, 526, 625, 680, 1299, 2022, 2373, 2498
SduI	GDGCHC	3 cut(s)	860, 980, 2202
SfaNI	GCATC	8 cut(s)	1207, 1244, 1353, 1384, 1614, 2271, 2283, 2449
SfcI	CTRYAG	5 cut(s)	1065, 1113, 1348, 1569, 1767
Sfr274I	CTCGAG	1 cut(s)	1262
SinI	GGWCC	5 cut(s)	100, 1497, 1580, 1756, 2494
SlaI	CTCGAG	1 cut(s)	1262
SmiMI	CAYNNNNRTG	2 cut(s)	35, 1091
SmlI	CTYRAG	3 cut(s)	222, 822, 1262
SmoI	CTYRAG	3 cut(s)	222, 822, 1262
SspI	AATATT	1 cut(s)	2162
SspMI	CTAG	8 cut(s)	186, 962, 981, 1172, 1545, 1913, 2048, 2259
SstI	GAGCTC	2 cut(s)	980, 2202
StyD4I	CCNGG	9 cut(s)	254, 310, 524, 623, 678, 1297, 2020, 2371, 2496
StyI	CCWWGG	6 cut(s)	10, 273, 409, 843, 2250, 2386
TaaI	ACNGT	5 cut(s)	200, 517, 1152, 1965, 2507
TaiI	ACGT	7 cut(s)	165, 342, 751, 1688, 1728, 2281, 2550
TaqI	TCGA	6 cut(s)	279, 1141, 1263, 1319, 2068, 2202
TatI	WGTACW	2 cut(s)	1483, 1573
TauI	GCSGC	4 cut(s)	663, 1326, 1853, 2465
TfiI	GAWTC	4 cut(s)	468, 529, 1712, 2153
Tru1I	TTAA	5 cut(s)	629, 1028, 1164, 1406, 2502
Tru9I	TTAA	5 cut(s)	629, 1028, 1164, 1406, 2502
TscAI	CASTG	3 cut(s)	178, 1463, 1573
TseFI	GTSAC	5 cut(s)	205, 232, 340, 1084, 1996
TseI	GCWGC	7 cut(s)	811, 1652, 1916, 2296, 2352, 2445, 2453
Tsp45I	GTSAC	5 cut(s)	205, 232, 340, 1084, 1996
TspDTI	ATGAA	8 cut(s)	446, 905, 1092, 1109, 1373, 1725, 2019, 2110
TspGWI	ACGGA	8 cut(s)	133, 667, 1378, 1446, 1721, 2015, 2205, 2229
TspRI	CASTG	3 cut(s)	178, 1463, 1573
Van91I	CCANNNNNTGG	3 cut(s)	541, 1508, 2027
VpaK11BI	GGWCC	5 cut(s)	100, 1497, 1580, 1756, 2494
XagI	CCTNNNNNAGG	1 cut(s)	1265
XapI	RAATTY	3 cut(s)	50, 997, 1157
XceI	RCATGY	2 cut(s)	9, 558
XcmI	CCANNNNNNNNNTGG	1 cut(s)	686
XhoI	CTCGAG	1 cut(s)	1262
XmnI	GAANNNNTTC	1 cut(s)	1945
XspI	CTAG	8 cut(s)	186, 962, 981, 1172, 1545, 1913, 2048, 2259
ZraI	GACGTC	1 cut(s)	2548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.