Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008970

Physical Location & Seq

Genomic Coordinates Reverse (-)

36439419 .. 36444914

Gene Structure 5496 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1309000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGATCTCCATTGCTTCTAATTCCGTTGAAACCCAAAAGCCGGCAGACACATCTACCAATCTCATGCCTCCTCTGATTAATTCTACCTCTCAACAAAACCTAACCCAATTGCAACCCCCCGCCTGCACCAACAATATTATTGATTCCACCACCGCCACCGCCACCATTAACCATGTATTCAGATCAAAACTACCAGACATACCCATCCCCAACCACCTCCCTCTCCACACCTACTGCTTCCAGAACCTCCCCGAGTTCTCCGATAGACCTTGCTTGATCGTGGGCTCCACCGGAAAATCATACTCTTTCTCCGAGACCCACCTCATTTCTCAGAAGACCGGCGCCGGCCTCTCCAATCTAGGCATCCAAAAAGGCGACGTCATCATGATCCTCCTTCAAAACTGCGCAGAGTTCGTCTTCGCTTTTATGGGCGCTTCCCTGATCGGCGCCGTTACCACCACCGCCAACCCCTTCTACACCTCTGCCGAGATTTTCAAGCAGGTCAAAGCCGCTAATGCCAAACTCATCATCACTCAATCCCAGTACGTCGATAAGCTCCGAGAACATCCCTCGTCCGCCGACGGTGCCGACCAAAATAACTACCCAAAACTCGGCGAAGACTTTAAGGTCGTCACGATTGACGATCCTCCGGAGAATTGCTTGCATTTCTCCGTGCTCTCCGAGGCCAACGAGAATGAGCTTCCGGACGTGGTGATCGACGCGGAGGACCCGGTGGCCCTACCGTTCTCTTCGGGGACCACGGGGCTCCCCAAGGGAGTCATTCTAACACACAAGAGCTTGGTCACCAGCGTGGCCCAGCAGGTGGACGGAGAGAATCCAAACCTCTACTTGAAGGAGGACGATGTCGTATTGTGCGTGCTGCCGTTGTTTCACATATTTTCGTTGAACAGCGTGCTGCTGTGCTCGCTGCGAGCAGGGGCCGGAGTTCTGCTGATGCACAAGTTTGAGATAGGTACGCTTCTGGAGCTGATTCAGCGGTACCGAGTGTCAGTGGCAGCGGTGGTGCCGCCGCTGGTTATAGCGCTGGCGAAGAACCCAATGGTGGCGGAGTTTGACCTGAGCTCTATTAGGGTGGTGTTGTCAGGAGCGGCGCCCCTGGGGAAGGAGCTGGAGGAGGCGCTCAAGAGCCGAGTCCCTCAGGCATTGTTGGGTCAGGGTTATGGGATGACGGAGGCAGGGCCGGTGCTGTCAATGTGCATGGCATTCGCAAAGGAACCGATGCCATCCAAGTCAGGGTCGTGTGGGACGGTGGTCCGAAATGCAGAGCTCAGGGTCCTTGACCCTGAAACTGGTCTGTCACTCGGCTATAACCAACCAGGCGAGATTTGCATCCGTGGCTCTCAAATTATGAAAGGATATTTGAATGACGCTGCGGCTACGGCAACCACCATAGACACGGAGGGCTGGCTTCACACCGGTGACGTGGGTTATGTGGATGATGACGATGAGGTTTTCATCGTTGACAGAGTCAAGGAGCTCATCAAATTCAAAGGCTTCCAAGTGCCACCAGCTGAGCTGGAGTCCCTCCTTATAAGCCACCCATCAATTGCAGATGCGGCCGTTGTTCCGCAAAAAGATGATGCTGCTGGTGAGGTTCCAGTTGCATTTGTGGTTCGGTCTGATGGTCTTGAACTTACTGAAGAGGCTGTAAAGGAATTTATAGCAAAACAGGTGGTGTTTTACAAGAGACTGCACAAGGTGCACTTTGTCCATGCAATTCCAAAGTCTGCGTCTGGAAAGATCTTGAGAAAAGACCTGAGAGCCAAGCTTGCCACCGCAACCCCACCGCTGCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009653 GO:0009698 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016207 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019748 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044237 GO:0045229 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071704 GO:0071840 GO:0085029 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

606

Amino Acids

65.19

Weight (kDa)

5.28

Isoelectric Point (pI)

33.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	84 - 461	1.1e-92	AMP-binding enzyme
AMP-binding_C	PF13193	512 - 587	1.3e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008970)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65060 AT1G65060
fragaria_vesca	FvH4_7g33990 FvH4_7g33990
malus_domestica	MD01G1236300.v1.1 MD07G1309000.v1.1
prunus_persica	Prupe.2G326300_v2.0.a1
pyrus_communis	pycom01g24420 pycom07g28100
rosa_chinensis	RchiOBHm_Chr1g0384231
rosa_laevigata	RLG00000026055
rosa_multiflora	Rmu_co8391797.1_g000001 Rmu_sc0011271.1_g000001
rosa_roxburghii	Rroxscaffold_4G00276820
rosa_rugosa	Rorug01G0446000
rosa_samantha	Rh1AG471500 Rh1BG429300 Rh1CG441900 Rh1DG462000 Rh6BG015700 Rh7CG464300
rosa_wichuraiana	Rw1G040100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1553
AarI	CACCTGC	1 cut(s)	811
AasI	GACNNNNNNGTC	1 cut(s)	626
AatII	GACGTC	1 cut(s)	381
Acc16I	TGCGCA	1 cut(s)	406
Acc36I	ACCTGC	2 cut(s)	490, 811
Acc65I	GGTACC	1 cut(s)	999
AccB1I	GGYRCC	6 cut(s)	341, 446, 584, 999, 1024, 1111
AccB7I	CCANNNNNTGG	1 cut(s)	823
AccBSI	CCGCTC	1 cut(s)	1109
AccII	CGCG	1 cut(s)	722
AccIII	TCCGGA	2 cut(s)	649, 703
AclWI	GGATC	2 cut(s)	382, 638
AcoI	YGGCCR	1 cut(s)	1578
AcsI	RAATTY	2 cut(s)	1505, 1676
AcuI	CTGAAG	1 cut(s)	1680
AcyI	GRCGYC	4 cut(s)	342, 378, 447, 1112
AdeI	CACNNNGTG	1 cut(s)	1720
AfaI	GTAC	3 cut(s)	545, 976, 1001
AfeI	AGCGCT	1 cut(s)	1044
AfiI	CCNNNNNNNGG	7 cut(s)	40, 611, 823, 1063, 1123, 1254, 1310
AgeI	ACCGGT	1 cut(s)	1436
AgsI	TTSAA	8 cut(s)	29, 398, 496, 853, 907, 1384, 1510, 1652
AjiI	CACGTC	2 cut(s)	709, 1444
AjnI	CCWGG	2 cut(s)	1116, 1336
AleI	CACNNNNGTG	2 cut(s)	809, 1437
Alw21I	GWGCWC	6 cut(s)	678, 926, 1085, 1290, 1500, 1725
Alw26I	GTCTC	2 cut(s)	308, 1702
Alw44I	GTGCAC	1 cut(s)	1721
AlwI	GGATC	2 cut(s)	382, 638
Ama87I	CYCGRG	1 cut(s)	251
Aor13HI	TCCGGA	2 cut(s)	649, 703
Aor51HI	AGCGCT	1 cut(s)	1044
AoxI	GGCC	7 cut(s)	346, 684, 735, 813, 939, 1199, 1578
ApaLI	GTGCAC	1 cut(s)	1721
ApeKI	GCWGC	7 cut(s)	880, 916, 928, 1016, 1391, 1604, 1810
ApoI	RAATTY	2 cut(s)	1505, 1676
ArsI	GACNNNNNNTTYG	2 cut(s)	1736, 1768
AseI	ATTAAT	1 cut(s)	78
AsiGI	ACCGGT	1 cut(s)	1436
Asp718I	GGTACC	1 cut(s)	999
AspLEI	GCGC	7 cut(s)	344, 407, 434, 449, 1045, 1114, 1141
AspS9I	GGNCC	8 cut(s)	727, 736, 756, 814, 939, 1199, 1273, 1294
AsuC2I	CCSGG	1 cut(s)	731
AsuHPI	GGTGA	4 cut(s)	724, 796, 1451, 1622
AvaI	CYCGRG	1 cut(s)	251
AvaII	GGWCC	4 cut(s)	727, 756, 1273, 1294
AxyI	CCTNAGG	1 cut(s)	1158
BaeGI	GKGCMC	1 cut(s)	1725
BanI	GGYRCC	6 cut(s)	341, 446, 584, 999, 1024, 1111
BanII	GRGCYC	5 cut(s)	287, 768, 1085, 1290, 1500
BbsI	GAAGAC	3 cut(s)	341, 409, 624
Bbv12I	GWGCWC	6 cut(s)	678, 926, 1085, 1290, 1500, 1725
BbvI	GCAGC	7 cut(s)	867, 903, 915, 1028, 1378, 1591, 1797
BccI	CCATC	4 cut(s)	212, 1252, 1570, 1637
BceAI	ACGGC	4 cut(s)	434, 868, 1416, 1565
BcgI	CGANNNNNNTGC	2 cut(s)	1207, 1241
BciT130I	CCWGG	2 cut(s)	1118, 1338
BcnI	CCSGG	1 cut(s)	731
BcoDI	GTCTC	2 cut(s)	308, 1702
BfaI	CTAG	1 cut(s)	359
BfoI	RGCGCY	6 cut(s)	345, 435, 450, 1046, 1115, 1142
BfuAI	ACCTGC	2 cut(s)	490, 811
BglII	AGATCT	1 cut(s)	1761
BlpI	GCTNAGC	1 cut(s)	1533
Bme1390I	CCNGG	3 cut(s)	731, 1118, 1338
Bme18I	GGWCC	4 cut(s)	727, 756, 1273, 1294
BmeT110I	CYCGRG	1 cut(s)	251
BmgBI	CACGTC	2 cut(s)	709, 1444
BmgT120I	GGNCC	8 cut(s)	727, 736, 756, 814, 939, 1199, 1273, 1294
BmrFI	CCNGG	3 cut(s)	731, 1118, 1338
BmrI	ACTGGG	1 cut(s)	535
BmsI	GCATC	6 cut(s)	372, 945, 1230, 1359, 1564, 1591
BmuI	ACTGGG	1 cut(s)	535
BoxI	GACNNNNGTC	1 cut(s)	1271
BpiI	GAAGAC	3 cut(s)	341, 409, 624
BpmI	CTGGAG	3 cut(s)	1004, 1151, 1559
Bpu10I	CCTNAGC	2 cut(s)	1079, 1289
Bpu1102I	GCTNAGC	1 cut(s)	1533
BpuEI	CTTGAG	2 cut(s)	1127, 1786
BpuMI	CCSGG	1 cut(s)	731
BsaBI	GATNNNNATC	1 cut(s)	1349
BsaHI	GRCGYC	4 cut(s)	342, 378, 447, 1112
BsaI	GGTCTC	1 cut(s)	308
BsaJI	CCNNGG	6 cut(s)	681, 759, 771, 1116, 1117, 1354
BsaWI	WCCGGW	4 cut(s)	290, 649, 703, 1436
BsaXI	ACNNNNNCTCC	2 cut(s)	293, 323
Bsc4I	CCNNNNNNNGG	7 cut(s)	40, 611, 823, 1063, 1123, 1254, 1310
Bse118I	RCCGGY	5 cut(s)	40, 338, 344, 1201, 1436
Bse1I	ACTGG	3 cut(s)	541, 1315, 1619
Bse21I	CCTNAGG	1 cut(s)	1158
Bse3DI	GCAATG	1 cut(s)	9
Bse8I	GATNNNNATC	1 cut(s)	1349
BseAI	TCCGGA	2 cut(s)	649, 703
BseBI	CCWGG	2 cut(s)	1118, 1338
BseDI	CCNNGG	6 cut(s)	681, 759, 771, 1116, 1117, 1354
BseGI	GGATG	7 cut(s)	204, 363, 565, 1191, 1244, 1350, 1462
BseJI	GATNNNNATC	1 cut(s)	1349
BseLI	CCNNNNNNNGG	7 cut(s)	40, 611, 823, 1063, 1123, 1254, 1310
BseMI	GCAATG	1 cut(s)	9
BseMII	CTCAG	6 cut(s)	344, 1070, 1172, 1303, 1524, 1769
BseNI	ACTGG	3 cut(s)	541, 1315, 1619
BseRI	GAGGAG	2 cut(s)	60, 1148
BseSI	GKGCMC	1 cut(s)	1725
BseX3I	CGGCCG	1 cut(s)	1578
BseXI	GCAGC	7 cut(s)	867, 903, 915, 1028, 1378, 1591, 1797
BseYI	CCCAGC	1 cut(s)	816
BsgI	GTGCAG	2 cut(s)	109, 1697
Bsh1236I	CGCG	1 cut(s)	722
Bsh1285I	CGRYCG	1 cut(s)	1581
BshFI	GGCC	7 cut(s)	348, 686, 737, 815, 941, 1201, 1580
BshNI	GGYRCC	6 cut(s)	341, 446, 584, 999, 1024, 1111
BshTI	ACCGGT	1 cut(s)	1436
BsiEI	CGRYCG	1 cut(s)	1581
BsiHKAI	GWGCWC	6 cut(s)	678, 926, 1085, 1290, 1500, 1725
BsiHKCI	CYCGRG	1 cut(s)	251
BslFI	GGGAC	4 cut(s)	769, 1139, 1279, 1528
BslI	CCNNNNNNNGG	7 cut(s)	40, 611, 823, 1063, 1123, 1254, 1310
BsmAI	GTCTC	2 cut(s)	308, 1702
BsmFI	GGGAC	4 cut(s)	769, 1139, 1279, 1528
BsmI	GAATGC	1 cut(s)	1223
BsnI	GGCC	7 cut(s)	348, 686, 737, 815, 941, 1201, 1580
Bso31I	GGTCTC	1 cut(s)	308
BsoBI	CYCGRG	1 cut(s)	251
Bsp1286I	GDGCHC	8 cut(s)	287, 678, 768, 926, 1085, 1290, 1500, 1725
Bsp13I	TCCGGA	2 cut(s)	649, 703
Bsp143I	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
Bsp1720I	GCTNAGC	1 cut(s)	1533
BspANI	GGCC	7 cut(s)	348, 686, 737, 815, 941, 1201, 1580
BspCNI	CTCAG	6 cut(s)	343, 1071, 1171, 1302, 1525, 1770
BspEI	TCCGGA	2 cut(s)	649, 703
BspFNI	CGCG	1 cut(s)	722
BspHI	TCATGA	1 cut(s)	384
BspMI	ACCTGC	2 cut(s)	490, 811
BspPI	GGATC	2 cut(s)	382, 638
BspT107I	GGYRCC	6 cut(s)	341, 446, 584, 999, 1024, 1111
BspTNI	GGTCTC	1 cut(s)	308
BsrBI	CCGCTC	1 cut(s)	1109
BsrDI	GCAATG	1 cut(s)	9
BsrFI	RCCGGY	5 cut(s)	40, 338, 344, 1201, 1436
BsrI	ACTGG	3 cut(s)	541, 1315, 1619
BssAI	RCCGGY	5 cut(s)	40, 338, 344, 1201, 1436
BssECI	CCNNGG	6 cut(s)	681, 759, 771, 1116, 1117, 1354
BssMI	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
BssNI	GRCGYC	4 cut(s)	342, 378, 447, 1112
BssT1I	CCWWGG	1 cut(s)	771
Bst2UI	CCWGG	2 cut(s)	1118, 1338
Bst4CI	ACNGT	3 cut(s)	584, 744, 1270
Bst6I	CTCTTC	2 cut(s)	754, 1656
BstACI	GRCGYC	4 cut(s)	342, 378, 447, 1112
BstAPI	GCANNNNNTGC	1 cut(s)	1720
BstDEI	CTNAG	6 cut(s)	330, 1079, 1158, 1289, 1533, 1778
BstDSI	CCRYGG	2 cut(s)	759, 1354
BstEII	GGTNACC	1 cut(s)	802
BstENI	CCTNNNNNAGG	1 cut(s)	1121
BstF5I	GGATG	7 cut(s)	204, 363, 565, 1191, 1244, 1350, 1462
BstFNI	CGCG	1 cut(s)	722
BstH2I	RGCGCY	6 cut(s)	345, 435, 450, 1046, 1115, 1142
BstHHI	GCGC	7 cut(s)	344, 407, 434, 449, 1045, 1114, 1141
BstKTI	GATC	8 cut(s)	6, 185, 279, 390, 444, 646, 717, 1764
BstMAI	GTCTC	2 cut(s)	308, 1702
BstMBI	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
BstMCI	CGRYCG	1 cut(s)	1581
BstNI	CCWGG	2 cut(s)	1118, 1338
BstPAI	GACNNNNGTC	1 cut(s)	1271
BstPI	GGTNACC	1 cut(s)	802
BstSCI	CCNGG	3 cut(s)	729, 1116, 1336
BstSLI	GKGCMC	1 cut(s)	1725
BstUI	CGCG	1 cut(s)	722
BstV1I	GCAGC	7 cut(s)	867, 903, 915, 1028, 1378, 1591, 1797
BstV2I	GAAGAC	3 cut(s)	341, 409, 624
BstX2I	RGATCY	1 cut(s)	1761
BstYI	RGATCY	1 cut(s)	1761
BstZI	CGGCCG	1 cut(s)	1578
Bsu36I	CCTNAGG	1 cut(s)	1158
BsuRI	GGCC	7 cut(s)	348, 686, 737, 815, 941, 1201, 1580
BtgI	CCRYGG	2 cut(s)	759, 1354
BtrI	CACGTC	2 cut(s)	709, 1444
BtsCI	GGATG	7 cut(s)	204, 363, 565, 1191, 1244, 1350, 1462
BtsIMutI	CAGTG	1 cut(s)	1017
BveI	ACCTGC	2 cut(s)	490, 811
CciI	TCATGA	1 cut(s)	384
CfoI	GCGC	7 cut(s)	344, 407, 434, 449, 1045, 1114, 1141
Cfr10I	RCCGGY	5 cut(s)	40, 338, 344, 1201, 1436
Cfr13I	GGNCC	8 cut(s)	727, 736, 756, 814, 939, 1199, 1273, 1294
CseI	GACGC	3 cut(s)	728, 1397, 1740
Csp6I	GTAC	3 cut(s)	544, 975, 1000
CspAI	ACCGGT	1 cut(s)	1436
CspCI	CAANNNNNGTGG	2 cut(s)	1674, 1709
CviAII	CATG	5 cut(s)	64, 173, 385, 1219, 1733
CviQI	GTAC	3 cut(s)	544, 975, 1000
DdeI	CTNAG	6 cut(s)	330, 1079, 1158, 1289, 1533, 1778
DinI	GGCGCC	3 cut(s)	343, 448, 1113
DpnI	GATC	8 cut(s)	5, 184, 278, 389, 443, 645, 716, 1763
DpnII	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
DraIII	CACNNNGTG	1 cut(s)	1720
DrdI	GACNNNNNNGTC	1 cut(s)	626
DseDI	GACNNNNNNGTC	1 cut(s)	626
EaeI	YGGCCR	1 cut(s)	1578
EagI	CGGCCG	1 cut(s)	1578
Eam1104I	CTCTTC	2 cut(s)	754, 1656
EarI	CTCTTC	2 cut(s)	754, 1656
EciI	GGCGGA	2 cut(s)	565, 1082
Ecl136II	GAGCTC	3 cut(s)	1083, 1288, 1498
EclXI	CGGCCG	1 cut(s)	1578
Eco130I	CCWWGG	1 cut(s)	771
Eco24I	GRGCYC	5 cut(s)	287, 768, 1085, 1290, 1500
Eco31I	GGTCTC	1 cut(s)	308
Eco47I	GGWCC	4 cut(s)	727, 756, 1273, 1294
Eco47III	AGCGCT	1 cut(s)	1044
Eco52I	CGGCCG	1 cut(s)	1578
Eco53kI	GAGCTC	3 cut(s)	1083, 1288, 1498
Eco57I	CTGAAG	1 cut(s)	1680
Eco81I	CCTNAGG	1 cut(s)	1158
Eco88I	CYCGRG	1 cut(s)	251
Eco91I	GGTNACC	1 cut(s)	802
EcoICRI	GAGCTC	3 cut(s)	1083, 1288, 1498
EcoNI	CCTNNNNNAGG	1 cut(s)	1121
EcoO109I	RGGNCCY	2 cut(s)	727, 1294
EcoO65I	GGTNACC	1 cut(s)	802
EcoRII	CCWGG	2 cut(s)	1116, 1336
EcoT14I	CCWWGG	1 cut(s)	771
EcoT38I	GRGCYC	5 cut(s)	287, 768, 1085, 1290, 1500
EgeI	GGCGCC	3 cut(s)	343, 448, 1113
EheI	GGCGCC	3 cut(s)	343, 448, 1113
ErhI	CCWWGG	1 cut(s)	771
FaeI	CATG	5 cut(s)	67, 176, 388, 1222, 1736
FalI	AAGNNNNNCTT	2 cut(s)	1709, 1741
FaqI	GGGAC	4 cut(s)	769, 1139, 1279, 1528
FatI	CATG	5 cut(s)	63, 172, 384, 1218, 1732
FauI	CCCGC	1 cut(s)	127
FokI	GGATG	7 cut(s)	191, 350, 552, 1198, 1231, 1337, 1469
FriOI	GRGCYC	5 cut(s)	287, 768, 1085, 1290, 1500
FspBI	CTAG	1 cut(s)	359
FspI	TGCGCA	1 cut(s)	406
GlaI	GCGC	7 cut(s)	343, 406, 433, 448, 1044, 1113, 1140
GsaI	CCCAGC	1 cut(s)	820
GsuI	CTGGAG	3 cut(s)	1004, 1151, 1559
HaeII	RGCGCY	6 cut(s)	345, 435, 450, 1046, 1115, 1142
HaeIII	GGCC	7 cut(s)	348, 686, 737, 815, 941, 1201, 1580
HgaI	GACGC	3 cut(s)	728, 1397, 1740
HhaI	GCGC	7 cut(s)	344, 407, 434, 449, 1045, 1114, 1141
Hin1I	GRCGYC	4 cut(s)	342, 378, 447, 1112
Hin1II	CATG	5 cut(s)	67, 176, 388, 1222, 1736
Hin6I	GCGC	7 cut(s)	342, 405, 432, 447, 1043, 1112, 1139
HinP1I	GCGC	7 cut(s)	342, 405, 432, 447, 1043, 1112, 1139
HincII	GTYRAC	1 cut(s)	1483
HindII	GTYRAC	1 cut(s)	1483
HindIII	AAGCTT	1 cut(s)	1787
HinfI	GANTC	7 cut(s)	143, 777, 835, 991, 1152, 1488, 1541
HphI	GGTGA	4 cut(s)	724, 796, 1451, 1622
Hpy166II	GTNNAC	3 cut(s)	826, 1483, 1723
Hpy188I	TCNGA	9 cut(s)	75, 182, 262, 313, 333, 560, 682, 1277, 1642
Hpy8I	GTNNAC	3 cut(s)	826, 1483, 1723
Hpy99I	CGWCG	4 cut(s)	380, 551, 584, 722
HpyAV	CCTTC	4 cut(s)	404, 481, 847, 1117
HpyCH4III	ACNGT	3 cut(s)	584, 744, 1270
HpyCH4IV	ACGT	4 cut(s)	378, 546, 708, 1443
HpyF3I	CTNAG	6 cut(s)	330, 1079, 1158, 1289, 1533, 1778
HpySE526I	ACGT	4 cut(s)	378, 546, 708, 1443
Hsp92I	GRCGYC	4 cut(s)	342, 378, 447, 1112
Hsp92II	CATG	5 cut(s)	67, 176, 388, 1222, 1736
HspAI	GCGC	7 cut(s)	342, 405, 432, 447, 1043, 1112, 1139
KasI	GGCGCC	3 cut(s)	341, 446, 1111
Kpn2I	TCCGGA	2 cut(s)	649, 703
KpnI	GGTACC	1 cut(s)	1003
KroI	GCCGGC	2 cut(s)	40, 344
KroNI	GCCGGC	2 cut(s)	42, 346
Kzo9I	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
LmnI	GCTCC	7 cut(s)	290, 561, 771, 985, 1106, 1126, 1495
Lsp1109I	GCAGC	7 cut(s)	867, 903, 915, 1028, 1378, 1591, 1797
LweI	GCATC	6 cut(s)	372, 945, 1230, 1359, 1564, 1591
MaeI	CTAG	1 cut(s)	359
MaeII	ACGT	4 cut(s)	378, 546, 708, 1443
MaeIII	GTNAC	5 cut(s)	451, 631, 802, 1317, 1439
MalI	GATC	8 cut(s)	5, 184, 278, 389, 443, 645, 716, 1763
MbiI	CCGCTC	1 cut(s)	1109
MboI	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
MboII	GAAGA	6 cut(s)	346, 409, 629, 741, 1063, 1673
MfeI	CAATTG	2 cut(s)	107, 1566
MflI	RGATCY	1 cut(s)	1761
MhlI	GDGCHC	8 cut(s)	287, 678, 768, 926, 1085, 1290, 1500, 1725
MluCI	AATT	9 cut(s)	19, 79, 107, 655, 1365, 1505, 1566, 1676, 1737
Mly113I	GGCGCC	3 cut(s)	342, 447, 1112
MlyI	GAGTC	4 cut(s)	786, 1161, 1497, 1550
MroI	TCCGGA	2 cut(s)	649, 703
MroNI	GCCGGC	2 cut(s)	40, 344
MseI	TTAA	3 cut(s)	78, 168, 624
MslI	CAYNNNNRTG	2 cut(s)	809, 1437
MspA1I	CMGCKG	5 cut(s)	997, 1019, 1033, 1532, 1810
MspR9I	CCNGG	3 cut(s)	731, 1118, 1338
MunI	CAATTG	2 cut(s)	107, 1566
Mva1269I	GAATGC	1 cut(s)	1223
MvaI	CCWGG	2 cut(s)	1118, 1338
MvnI	CGCG	1 cut(s)	722
NaeI	GCCGGC	2 cut(s)	42, 346
NarI	GGCGCC	3 cut(s)	342, 447, 1112
NciI	CCSGG	1 cut(s)	731
NdeII	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
NgoMIV	GCCGGC	2 cut(s)	40, 344
NlaIII	CATG	5 cut(s)	67, 176, 388, 1222, 1736
NmeAIII	GCCGAG	4 cut(s)	511, 591, 1175, 1302
NmuCI	GTSAC	4 cut(s)	631, 802, 1317, 1439
NsbI	TGCGCA	1 cut(s)	406
OliI	CACNNNNGTG	2 cut(s)	809, 1437
PagI	TCATGA	1 cut(s)	384
PaqCI	CACCTGC	1 cut(s)	811
PasI	CCCWGGG	1 cut(s)	1117
PcsI	WCGNNNNNNNCGW	2 cut(s)	714, 873
PctI	GAATGC	1 cut(s)	1223
PdiI	GCCGGC	2 cut(s)	42, 346
PfeI	GAWTC	3 cut(s)	143, 835, 991
PflFI	GACNNNGTC	2 cut(s)	863, 1487
PflMI	CCANNNNNTGG	1 cut(s)	823
PinAI	ACCGGT	1 cut(s)	1436
PleI	GAGTC	4 cut(s)	785, 1160, 1496, 1549
PluTI	GGCGCC	3 cut(s)	345, 450, 1115
PpsI	GAGTC	4 cut(s)	785, 1160, 1496, 1549
PpuMI	RGGWCCY	2 cut(s)	727, 1294
PshAI	GACNNNNGTC	1 cut(s)	1271
PshBI	ATTAAT	1 cut(s)	78
PsiI	TTATAA	1 cut(s)	1553
Psp124BI	GAGCTC	3 cut(s)	1085, 1290, 1500
Psp5II	RGGWCCY	2 cut(s)	727, 1294
Psp6I	CCWGG	2 cut(s)	1116, 1336
PspEI	GGTNACC	1 cut(s)	802
PspFI	CCCAGC	1 cut(s)	816
PspGI	CCWGG	2 cut(s)	1116, 1336
PspPI	GGNCC	8 cut(s)	727, 736, 756, 814, 939, 1199, 1273, 1294
PspPPI	RGGWCCY	2 cut(s)	727, 1294
PsuI	RGATCY	1 cut(s)	1761
PsyI	GACNNNGTC	2 cut(s)	863, 1487
PvuII	CAGCTG	1 cut(s)	1532
RsaI	GTAC	3 cut(s)	545, 976, 1001
RsaNI	GTAC	3 cut(s)	544, 975, 1000
RseI	CAYNNNNRTG	2 cut(s)	809, 1437
SacI	GAGCTC	3 cut(s)	1085, 1290, 1500
SaqAI	TTAA	3 cut(s)	78, 168, 624
Sau3AI	GATC	8 cut(s)	3, 182, 276, 387, 441, 643, 714, 1761
Sau96I	GGNCC	8 cut(s)	727, 736, 756, 814, 939, 1199, 1273, 1294
SchI	GAGTC	4 cut(s)	786, 1161, 1497, 1550
ScrFI	CCNGG	3 cut(s)	731, 1118, 1338
SduI	GDGCHC	8 cut(s)	287, 678, 768, 926, 1085, 1290, 1500, 1725
SfaNI	GCATC	6 cut(s)	372, 945, 1230, 1359, 1564, 1591
SfoI	GGCGCC	3 cut(s)	343, 448, 1113
SgrAI	CRCCGGYG	1 cut(s)	1436
SinI	GGWCC	4 cut(s)	727, 756, 1273, 1294
SmiMI	CAYNNNNRTG	2 cut(s)	809, 1437
SmlI	CTYRAG	2 cut(s)	1142, 1765
SmoI	CTYRAG	2 cut(s)	1142, 1765
Sse9I	AATT	9 cut(s)	19, 79, 107, 655, 1365, 1505, 1566, 1676, 1737
SspDI	GGCGCC	3 cut(s)	341, 446, 1111
SspI	AATATT	1 cut(s)	136
SspMI	CTAG	1 cut(s)	359
SstI	GAGCTC	3 cut(s)	1085, 1290, 1500
StyD4I	CCNGG	3 cut(s)	729, 1116, 1336
StyI	CCWWGG	1 cut(s)	771
TaaI	ACNGT	3 cut(s)	584, 744, 1270
TaiI	ACGT	4 cut(s)	381, 549, 711, 1446
TaqI	TCGA	2 cut(s)	549, 717
TaqII	GACCGA	1 cut(s)	1626
TasI	AATT	9 cut(s)	19, 79, 107, 655, 1365, 1505, 1566, 1676, 1737
TauI	GCSGC	6 cut(s)	512, 1030, 1033, 1112, 1397, 1580
TfiI	GAWTC	3 cut(s)	143, 835, 991
Tru1I	TTAA	3 cut(s)	78, 168, 624
Tru9I	TTAA	3 cut(s)	78, 168, 624
TscAI	CASTG	1 cut(s)	1017
TseFI	GTSAC	4 cut(s)	631, 802, 1317, 1439
TseI	GCWGC	7 cut(s)	880, 916, 928, 1016, 1391, 1604, 1810
Tsp45I	GTSAC	4 cut(s)	631, 802, 1317, 1439
TspDTI	ATGAA	2 cut(s)	1385, 1465
TspGWI	ACGGA	6 cut(s)	13, 661, 843, 1205, 1343, 1433
TspRI	CASTG	1 cut(s)	1017
Tth111I	GACNNNGTC	2 cut(s)	863, 1487
Van91I	CCANNNNNTGG	1 cut(s)	823
VneI	GTGCAC	1 cut(s)	1721
VpaK11BI	GGWCC	4 cut(s)	727, 756, 1273, 1294
VspI	ATTAAT	1 cut(s)	78
XagI	CCTNNNNNAGG	1 cut(s)	1121
XapI	RAATTY	2 cut(s)	1505, 1676
XspI	CTAG	1 cut(s)	359
ZraI	GACGTC	1 cut(s)	379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.