Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0008970

Physical Location & Seq

Genomic Coordinates Forward (+)

299265 .. 303233

Gene Structure 3969 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00276820.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGATATCCATTGCCTCTAACAACAACAACAGAGTTGTTGTTGAGACGCCGGAAATCTCTCCCACTCTCATCTCCGACGTCGTTTCCACCTCTCTGACCCAACCGGAGAAGCAGCAGCCAACCACCACTCATCATGATCATGTCTTCAAATCCAAGCTTCCGGACCTTCCCAACATCTCCAACCACCTCCCTCTCCACACCTACTGCTTCGAAAACATCTCCGACTTCTCCGACCGACCCTGTCTCATCGTCGGCTCCACCGGAAAAACATACACCTTCTCCGAAACCCACCTCATTTCCCAAAAGACCGGAGCCGGTCTCTCCAAGCTTGGCATCACCAAAGGCGATGTCGTCATGATCCTCCTCCCAAACTGCGCCGAGTTCACCTTCGCATTCATGGGAGCTTCCATGATCGGCGCCGTCACAACCACCGCCAACCCCTTCTACACAGCCTCCGAAATCTTCAAGCAGTTCAAGGCCTCCAACGCCAAGCTCATCATCACTCAATCCATATACGTAGACAAGCTCCGACACTCCGGTACCAGCTCTGATCAATACCCGATGCTCGGCCAACACTTTCAAGTGGTCACCATCGACGACCCTCCCATGAACTGCTTACATTTCTCAGTCCTCTCAGACGCCAACGAAAACGAACTTCCGGAGGTTTCTATTGATCCAGAGGACCCTGTAGCGCTACCTTTCTCTTCAGGAACTACAGGGCTTCCAAAAGGAGTGATTCTAACTCACAAGAGTCTGATCACAAGTGTGGCTCAGCAAGTGGACGGAGAGAATCCGAACCTGTACTTGAAGGAAGACGATGTCGTTTTGTGCGTGCTGCCATTGTTCCATATCTACTCGTTGAACAGTGTCTTGTTGTGCTCTCTCAGAGCAGGGGCGGCGGTTTTGTTGATGCACAAGTTTGAGATTGGGACGCTTTTGGAGCTCATACAGAGGTACAGGGTGTCTGTGGCGGCGGTGGTGCCACCGCTGGTGATAGCGCTGGCTAAGAATCCGATGGTGGGGGACTATGATCTCAGCTCTATTAGAGCGATCTTGTCCGGAGCGGCGCCGCTGGGAAAGGAGCTAGAGGAGGCGTTGAGGAGCCGTGTGCCTCAGGCAGTCTTGGGCCAGGGTTATGGGATGACAGAGGCAGGGCCGGTGCTATCAATGTGCCTGGCATTCGCAAAGCAACCGTTTCCAACGAAGTCAGGGTCATGCGGCACCGTGGTTCGAAATGCGGAGCTCAAAGTCATCGAACCCGAAACTGGTCGCTCCCTCGGCTACAACCAGCCTGGCGAGATTTGCATCCGTGGCTCTCAAATTATGAAAGGATATTTGAACGACGATGAGGCCACGGCAACCACCGTAGATGTCGAGGGATGGCTTCACACCGGCGACGTTGGTTATGTGGACGATGACGATGAGGTTTTCATCGTTGACAGAGTTAAGGAGCTCATCAAATTCAAAGGCTTCCAAGTGCCACCAGCTGAACTAGAAGCCCTCCTTGTAAGCCACCAATCCATTGCAGATGCAGCTGTTGTTCCGCAAAAAGATGACGCTGCTGGGGAAGTTCCGGTTGCATTCGTGGTTCGATCTAATGGTAATGAACTTACCGAAGAGGCTATAAAAGAATTTATAGCAAAGCAGGTGGTGTTTTACAAGAGACTGCACAAGGTCTACTTCGTGCATGCAATTCCGAAATCTCCGTCTGGAAAAATCTTAAGAAAAGACCTCAGAGCCAAGCTAGCTACTCCAAATGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009653 GO:0009698 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016207 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019748 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044237 GO:0045229 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071704 GO:0071840 GO:0085029 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

587

Amino Acids

63.79

Weight (kDa)

5.56

Isoelectric Point (pI)

35.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	71 - 446	1.5e-94	AMP-binding enzyme
AMP-binding_C	PF13193	497 - 572	6.8e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008970)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65060 AT1G65060
fragaria_vesca	FvH4_7g33990 FvH4_7g33990
malus_domestica	MD01G1236300.v1.1 MD07G1309000.v1.1
prunus_persica	Prupe.2G326300_v2.0.a1
pyrus_communis	pycom01g24420 pycom07g28100
rosa_chinensis	RchiOBHm_Chr1g0384231
rosa_laevigata	RLG00000026055
rosa_multiflora	Rmu_co8391797.1_g000001 Rmu_sc0011271.1_g000001
rosa_roxburghii	Rroxscaffold_4G00276820
rosa_rugosa	Rorug01G0446000
rosa_samantha	Rh1AG471500 Rh1BG429300 Rh1CG441900 Rh1DG462000 Rh6BG015700 Rh7CG464300
rosa_wichuraiana	Rw1G040100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1636
AatII	GACGTC	1 cut(s)	81
Acc36I	ACCTGC	1 cut(s)	1636
Acc65I	GGTACC	1 cut(s)	539
AccB1I	GGYRCC	5 cut(s)	416, 539, 979, 1066, 1220
AccBSI	CCGCTC	1 cut(s)	1064
AccI	GTMKAC	2 cut(s)	519, 1677
AccIII	TCCGGA	3 cut(s)	160, 658, 1058
AclWI	GGATC	2 cut(s)	352, 668
AcoI	YGGCCR	1 cut(s)	568
AcsI	RAATTY	2 cut(s)	1460, 1631
AcuI	CTGAAG	1 cut(s)	690
AcyI	GRCGYC	5 cut(s)	47, 78, 417, 639, 1067
AfaI	GTAC	3 cut(s)	541, 803, 956
AfeI	AGCGCT	2 cut(s)	693, 999
AfiI	CCNNNNNNNGG	3 cut(s)	566, 1019, 1265
AflII	CTTAAG	1 cut(s)	1720
AgsI	TTSAA	8 cut(s)	148, 466, 475, 581, 808, 862, 1339, 1465
AjnI	CCWGG	3 cut(s)	1128, 1173, 1291
AleI	CACNNNNGTG	1 cut(s)	764
Alw21I	GWGCWC	4 cut(s)	881, 945, 1245, 1455
Alw26I	GTCTC	4 cut(s)	38, 248, 323, 1657
AlwI	GGATC	2 cut(s)	352, 668
Aor13HI	TCCGGA	3 cut(s)	160, 658, 1058
Aor51HI	AGCGCT	2 cut(s)	693, 999
AoxI	GGCC	5 cut(s)	477, 568, 1126, 1154, 1350
ApeKI	GCWGC	5 cut(s)	112, 115, 835, 1532, 1559
ApoI	RAATTY	2 cut(s)	1460, 1631
ArsI	GACNNNNNNTTYG	6 cut(s)	333, 365, 1196, 1228, 1691, 1723
Asp718I	GGTACC	1 cut(s)	539
AspLEI	GCGC	5 cut(s)	377, 419, 694, 1000, 1069
AspS9I	GGNCC	4 cut(s)	163, 682, 1126, 1154
AsuHPI	GGTGA	4 cut(s)	328, 376, 580, 1003
AsuII	TTCGAA	2 cut(s)	210, 1231
AsuNHI	GCTAGC	1 cut(s)	1744
AvaII	GGWCC	2 cut(s)	163, 682
AxyI	CCTNAGG	1 cut(s)	1113
BaeI	ACNNNNGTAYC	2 cut(s)	523, 556
BanI	GGYRCC	5 cut(s)	416, 539, 979, 1066, 1220
BanII	GRGCYC	3 cut(s)	945, 1245, 1455
BbsI	GAAGAC	2 cut(s)	136, 819
Bbv12I	GWGCWC	4 cut(s)	881, 945, 1245, 1455
BbvI	GCAGC	5 cut(s)	124, 127, 822, 1544, 1546
BccI	CCATC	3 cut(s)	599, 1009, 1374
BceAI	ACGGC	3 cut(s)	404, 1089, 1371
BciT130I	CCWGG	3 cut(s)	1130, 1175, 1293
BclI	TGATCA	3 cut(s)	136, 550, 756
BcoDI	GTCTC	4 cut(s)	38, 248, 323, 1657
BfaI	CTAG	3 cut(s)	1085, 1493, 1745
BfmI	CTRYAG	2 cut(s)	687, 714
BfoI	RGCGCY	4 cut(s)	420, 695, 1001, 1070
BfrI	CTTAAG	1 cut(s)	1720
BfuAI	ACCTGC	1 cut(s)	1636
BlpI	GCTNAGC	1 cut(s)	771
Bme1390I	CCNGG	3 cut(s)	1130, 1175, 1293
Bme18I	GGWCC	2 cut(s)	163, 682
BmgT120I	GGNCC	4 cut(s)	163, 682, 1126, 1154
BmiI	GGNNCC	9 cut(s)	256, 313, 418, 541, 684, 981, 1068, 1103, 1222
BmrFI	CCNGG	3 cut(s)	1130, 1175, 1293
BmsI	GCATC	5 cut(s)	342, 552, 900, 1314, 1519
BmtI	GCTAGC	1 cut(s)	1748
BpiI	GAAGAC	2 cut(s)	136, 819
BplI	GAGNNNNNCTC	2 cut(s)	303, 335
Bpu1102I	GCTNAGC	1 cut(s)	771
Bpu14I	TTCGAA	2 cut(s)	210, 1231
BsaAI	YACGTR	1 cut(s)	517
BsaBI	GATNNNNATC	1 cut(s)	1304
BsaHI	GRCGYC	5 cut(s)	47, 78, 417, 639, 1067
BsaI	GGTCTC	1 cut(s)	323
BsaJI	CCNNGG	5 cut(s)	1129, 1224, 1276, 1309, 1353
BsaWI	WCCGGW	8 cut(s)	103, 160, 260, 308, 536, 658, 1058, 1573
BsaXI	ACNNNNNCTCC	4 cut(s)	263, 293, 437, 467
Bsc4I	CCNNNNNNNGG	3 cut(s)	566, 1019, 1265
Bse118I	RCCGGY	3 cut(s)	314, 1156, 1391
Bse1I	ACTGG	1 cut(s)	1270
Bse21I	CCTNAGG	1 cut(s)	1113
Bse3DI	GCAATG	2 cut(s)	9, 1521
Bse8I	GATNNNNATC	1 cut(s)	1304
BseAI	TCCGGA	3 cut(s)	160, 658, 1058
BseBI	CCWGG	3 cut(s)	1130, 1175, 1293
BseDI	CCNNGG	5 cut(s)	1129, 1224, 1276, 1309, 1353
BseGI	GGATG	3 cut(s)	1146, 1305, 1385
BseJI	GATNNNNATC	1 cut(s)	1304
BseLI	CCNNNNNNNGG	3 cut(s)	566, 1019, 1265
BseMI	GCAATG	2 cut(s)	9, 1521
BseMII	CTCAG	7 cut(s)	639, 648, 785, 898, 1048, 1127, 1747
BseNI	ACTGG	1 cut(s)	1270
BseRI	GAGGAG	3 cut(s)	353, 1103, 1114
BseXI	GCAGC	5 cut(s)	124, 127, 822, 1544, 1546
BseYI	CCCAGC	2 cut(s)	1072, 1562
BsgI	GTGCAG	1 cut(s)	1652
Bsh1285I	CGRYCG	1 cut(s)	235
BshFI	GGCC	5 cut(s)	479, 570, 1128, 1156, 1352
BshNI	GGYRCC	5 cut(s)	416, 539, 979, 1066, 1220
BsiEI	CGRYCG	1 cut(s)	235
BsiHKAI	GWGCWC	4 cut(s)	881, 945, 1245, 1455
BslFI	GGGAC	2 cut(s)	943, 1037
BslI	CCNNNNNNNGG	3 cut(s)	566, 1019, 1265
BsmAI	GTCTC	4 cut(s)	38, 248, 323, 1657
BsmBI	CGTCTC	1 cut(s)	38
BsmFI	GGGAC	2 cut(s)	943, 1037
BsmI	GAATGC	3 cut(s)	392, 1178, 1580
BsnI	GGCC	5 cut(s)	479, 570, 1128, 1156, 1352
Bso31I	GGTCTC	1 cut(s)	323
Bsp119I	TTCGAA	2 cut(s)	210, 1231
Bsp1286I	GDGCHC	4 cut(s)	881, 945, 1245, 1455
Bsp13I	TCCGGA	3 cut(s)	160, 658, 1058
Bsp143I	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
Bsp1720I	GCTNAGC	1 cut(s)	771
BspANI	GGCC	5 cut(s)	479, 570, 1128, 1156, 1352
BspCNI	CTCAG	7 cut(s)	638, 647, 784, 897, 1047, 1126, 1746
BspEI	TCCGGA	3 cut(s)	160, 658, 1058
BspHI	TCATGA	2 cut(s)	133, 354
BspLI	GGNNCC	9 cut(s)	256, 313, 418, 541, 684, 981, 1068, 1103, 1222
BspMI	ACCTGC	1 cut(s)	1636
BspOI	GCTAGC	1 cut(s)	1748
BspPI	GGATC	2 cut(s)	352, 668
BspT104I	TTCGAA	2 cut(s)	210, 1231
BspT107I	GGYRCC	5 cut(s)	416, 539, 979, 1066, 1220
BspTI	CTTAAG	1 cut(s)	1720
BspTNI	GGTCTC	1 cut(s)	323
BsrBI	CCGCTC	1 cut(s)	1064
BsrDI	GCAATG	2 cut(s)	9, 1521
BsrFI	RCCGGY	3 cut(s)	314, 1156, 1391
BsrI	ACTGG	1 cut(s)	1270
BssAI	RCCGGY	3 cut(s)	314, 1156, 1391
BssECI	CCNNGG	5 cut(s)	1129, 1224, 1276, 1309, 1353
BssMI	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
BssNI	GRCGYC	5 cut(s)	47, 78, 417, 639, 1067
Bst2UI	CCWGG	3 cut(s)	1130, 1175, 1293
Bst4CI	ACNGT	4 cut(s)	866, 1194, 1225, 1366
Bst6I	CTCTTC	2 cut(s)	709, 1611
BstACI	GRCGYC	5 cut(s)	47, 78, 417, 639, 1067
BstAFI	CTTAAG	1 cut(s)	1720
BstBAI	YACGTR	1 cut(s)	517
BstBI	TTCGAA	2 cut(s)	210, 1231
BstC8I	GCNNGC	4 cut(s)	833, 1002, 1689, 1746
BstDEI	CTNAG	8 cut(s)	625, 634, 771, 884, 1005, 1034, 1113, 1733
BstDSI	CCRYGG	3 cut(s)	1224, 1309, 1353
BstEII	GGTNACC	1 cut(s)	586
BstF5I	GGATG	3 cut(s)	1146, 1305, 1385
BstH2I	RGCGCY	4 cut(s)	420, 695, 1001, 1070
BstHHI	GCGC	5 cut(s)	377, 419, 694, 1000, 1069
BstKTI	GATC	9 cut(s)	139, 360, 414, 553, 676, 759, 1033, 1053, 1595
BstMAI	GTCTC	4 cut(s)	38, 248, 323, 1657
BstMBI	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
BstMCI	CGRYCG	1 cut(s)	235
BstMWI	GCNNNNNNNGC	8 cut(s)	485, 896, 940, 1278, 1302, 1311, 1532, 1745
BstNI	CCWGG	3 cut(s)	1130, 1175, 1293
BstNSI	RCATGY	1 cut(s)	1691
BstPI	GGTNACC	1 cut(s)	586
BstSCI	CCNGG	3 cut(s)	1128, 1173, 1291
BstSFI	CTRYAG	2 cut(s)	687, 714
BstSNI	TACGTA	1 cut(s)	517
BstV1I	GCAGC	5 cut(s)	124, 127, 822, 1544, 1546
BstV2I	GAAGAC	2 cut(s)	136, 819
BstXI	CCANNNNNNTGG	1 cut(s)	1136
Bsu36I	CCTNAGG	1 cut(s)	1113
BsuRI	GGCC	5 cut(s)	479, 570, 1128, 1156, 1352
BtgI	CCRYGG	3 cut(s)	1224, 1309, 1353
BtgZI	GCGATG	1 cut(s)	360
BtsCI	GGATG	3 cut(s)	1146, 1305, 1385
BtsIMutI	CAGTG	1 cut(s)	871
BveI	ACCTGC	1 cut(s)	1636
Cac8I	GCNNGC	4 cut(s)	833, 1002, 1689, 1746
CciI	TCATGA	2 cut(s)	133, 354
CfoI	GCGC	5 cut(s)	377, 419, 694, 1000, 1069
Cfr10I	RCCGGY	3 cut(s)	314, 1156, 1391
Cfr13I	GGNCC	4 cut(s)	163, 682, 1126, 1154
CseI	GACGC	4 cut(s)	55, 647, 940, 1565
Csp6I	GTAC	3 cut(s)	540, 802, 955
CspCI	CAANNNNNGTGG	2 cut(s)	1629, 1664
CviAII	CATG	8 cut(s)	134, 140, 355, 397, 409, 607, 1215, 1688
CviQI	GTAC	3 cut(s)	540, 802, 955
DdeI	CTNAG	8 cut(s)	625, 634, 771, 884, 1005, 1034, 1113, 1733
DinI	GGCGCC	2 cut(s)	418, 1068
DpnI	GATC	9 cut(s)	138, 359, 413, 552, 675, 758, 1032, 1052, 1594
DpnII	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
EaeI	YGGCCR	1 cut(s)	568
Eam1104I	CTCTTC	2 cut(s)	709, 1611
EarI	CTCTTC	2 cut(s)	709, 1611
Ecl136II	GAGCTC	3 cut(s)	943, 1243, 1453
Eco105I	TACGTA	1 cut(s)	517
Eco147I	AGGCCT	1 cut(s)	479
Eco24I	GRGCYC	3 cut(s)	945, 1245, 1455
Eco31I	GGTCTC	1 cut(s)	323
Eco32I	GATATC	1 cut(s)	6
Eco47I	GGWCC	2 cut(s)	163, 682
Eco47III	AGCGCT	2 cut(s)	693, 999
Eco53kI	GAGCTC	3 cut(s)	943, 1243, 1453
Eco57I	CTGAAG	1 cut(s)	690
Eco81I	CCTNAGG	1 cut(s)	1113
Eco91I	GGTNACC	1 cut(s)	586
EcoICRI	GAGCTC	3 cut(s)	943, 1243, 1453
EcoO109I	RGGNCCY	1 cut(s)	682
EcoO65I	GGTNACC	1 cut(s)	586
EcoRII	CCWGG	3 cut(s)	1128, 1173, 1291
EcoRV	GATATC	1 cut(s)	6
EcoT38I	GRGCYC	3 cut(s)	945, 1245, 1455
EgeI	GGCGCC	2 cut(s)	418, 1068
EheI	GGCGCC	2 cut(s)	418, 1068
Esp3I	CGTCTC	1 cut(s)	38
FaeI	CATG	8 cut(s)	137, 143, 358, 400, 412, 610, 1218, 1691
FalI	AAGNNNNNCTT	4 cut(s)	1488, 1520, 1664, 1696
FaqI	GGGAC	2 cut(s)	943, 1037
FatI	CATG	8 cut(s)	133, 139, 354, 396, 408, 606, 1214, 1687
FbaI	TGATCA	3 cut(s)	136, 550, 756
FblI	GTMKAC	2 cut(s)	519, 1677
FokI	GGATG	3 cut(s)	1153, 1292, 1392
FriOI	GRGCYC	3 cut(s)	945, 1245, 1455
FspBI	CTAG	3 cut(s)	1085, 1493, 1745
GlaI	GCGC	5 cut(s)	376, 418, 693, 999, 1068
GsaI	CCCAGC	2 cut(s)	1076, 1566
HaeII	RGCGCY	4 cut(s)	420, 695, 1001, 1070
HaeIII	GGCC	5 cut(s)	479, 570, 1128, 1156, 1352
HgaI	GACGC	4 cut(s)	55, 647, 940, 1565
HhaI	GCGC	5 cut(s)	377, 419, 694, 1000, 1069
Hin1I	GRCGYC	5 cut(s)	47, 78, 417, 639, 1067
Hin1II	CATG	8 cut(s)	137, 143, 358, 400, 412, 610, 1218, 1691
Hin6I	GCGC	5 cut(s)	375, 417, 692, 998, 1067
HinP1I	GCGC	5 cut(s)	375, 417, 692, 998, 1067
HincII	GTYRAC	1 cut(s)	1438
HindII	GTYRAC	1 cut(s)	1438
HindIII	AAGCTT	2 cut(s)	155, 326
HinfI	GANTC	4 cut(s)	736, 751, 790, 1009
HphI	GGTGA	4 cut(s)	328, 376, 580, 1003
Hpy166II	GTNNAC	6 cut(s)	384, 520, 781, 1411, 1438, 1678
Hpy188III	TCNNGA	8 cut(s)	134, 161, 355, 659, 677, 708, 1059, 1710
Hpy8I	GTNNAC	6 cut(s)	384, 520, 781, 1411, 1438, 1678
Hpy99I	CGWCG	6 cut(s)	80, 83, 254, 599, 1346, 1400
HpyAV	CCTTC	5 cut(s)	176, 286, 397, 451, 802
HpyCH4III	ACNGT	4 cut(s)	866, 1194, 1225, 1366
HpyCH4IV	ACGT	3 cut(s)	78, 516, 1398
HpyCH4V	TGCA	8 cut(s)	913, 1305, 1526, 1532, 1580, 1669, 1687, 1691
HpyF10VI	GCNNNNNNNGC	8 cut(s)	485, 896, 940, 1278, 1302, 1311, 1532, 1745
HpyF3I	CTNAG	8 cut(s)	625, 634, 771, 884, 1005, 1034, 1113, 1733
HpySE526I	ACGT	3 cut(s)	78, 516, 1398
Hsp92I	GRCGYC	5 cut(s)	47, 78, 417, 639, 1067
Hsp92II	CATG	8 cut(s)	137, 143, 358, 400, 412, 610, 1218, 1691
HspAI	GCGC	5 cut(s)	375, 417, 692, 998, 1067
KasI	GGCGCC	2 cut(s)	416, 1066
Kpn2I	TCCGGA	3 cut(s)	160, 658, 1058
KpnI	GGTACC	1 cut(s)	543
Ksp22I	TGATCA	3 cut(s)	136, 550, 756
Kzo9I	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
Lsp1109I	GCAGC	5 cut(s)	124, 127, 822, 1544, 1546
LweI	GCATC	5 cut(s)	342, 552, 900, 1314, 1519
MaeI	CTAG	3 cut(s)	1085, 1493, 1745
MaeII	ACGT	3 cut(s)	78, 516, 1398
MaeIII	GTNAC	2 cut(s)	421, 586
MalI	GATC	9 cut(s)	138, 359, 413, 552, 675, 758, 1032, 1052, 1594
MbiI	CCGCTC	1 cut(s)	1064
MboI	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
MboII	GAAGA	5 cut(s)	136, 454, 696, 824, 1628
MhlI	GDGCHC	4 cut(s)	881, 945, 1245, 1455
MluCI	AATT	4 cut(s)	1320, 1460, 1631, 1692
Mly113I	GGCGCC	2 cut(s)	417, 1067
MlyI	GAGTC	1 cut(s)	760
MmeI	TCCRAC	7 cut(s)	99, 204, 246, 255, 507, 553, 1223
MroI	TCCGGA	3 cut(s)	160, 658, 1058
MseI	TTAA	2 cut(s)	1446, 1721
MslI	CAYNNNNRTG	2 cut(s)	138, 764
MspA1I	CMGCKG	4 cut(s)	988, 1072, 1487, 1535
MspCI	CTTAAG	1 cut(s)	1720
MspR9I	CCNGG	3 cut(s)	1130, 1175, 1293
Mva1269I	GAATGC	3 cut(s)	392, 1178, 1580
MvaI	CCWGG	3 cut(s)	1130, 1175, 1293
MwoI	GCNNNNNNNGC	8 cut(s)	485, 896, 940, 1278, 1302, 1311, 1532, 1745
NarI	GGCGCC	2 cut(s)	417, 1067
NdeII	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
NheI	GCTAGC	1 cut(s)	1744
NlaIII	CATG	8 cut(s)	137, 143, 358, 400, 412, 610, 1218, 1691
NlaIV	GGNNCC	9 cut(s)	256, 313, 418, 541, 684, 981, 1068, 1103, 1222
NmeAIII	GCCGAG	3 cut(s)	403, 546, 1257
NmuCI	GTSAC	2 cut(s)	421, 586
NspI	RCATGY	1 cut(s)	1691
NspV	TTCGAA	2 cut(s)	210, 1231
OliI	CACNNNNGTG	1 cut(s)	764
PaeI	GCATGC	1 cut(s)	1691
PagI	TCATGA	2 cut(s)	133, 354
PaqCI	CACCTGC	1 cut(s)	1636
PceI	AGGCCT	1 cut(s)	479
PcsI	WCGNNNNNNNCGW	1 cut(s)	828
PctI	GAATGC	3 cut(s)	392, 1178, 1580
PfeI	GAWTC	3 cut(s)	736, 790, 1009
PflFI	GACNNNGTC	2 cut(s)	240, 818
PleI	GAGTC	1 cut(s)	759
PluTI	GGCGCC	2 cut(s)	420, 1070
PpsI	GAGTC	1 cut(s)	759
Ppu21I	YACGTR	1 cut(s)	517
PpuMI	RGGWCCY	1 cut(s)	682
Psp124BI	GAGCTC	3 cut(s)	945, 1245, 1455
Psp5II	RGGWCCY	1 cut(s)	682
Psp6I	CCWGG	3 cut(s)	1128, 1173, 1291
PspEI	GGTNACC	1 cut(s)	586
PspFI	CCCAGC	2 cut(s)	1072, 1562
PspGI	CCWGG	3 cut(s)	1128, 1173, 1291
PspN4I	GGNNCC	9 cut(s)	256, 313, 418, 541, 684, 981, 1068, 1103, 1222
PspPI	GGNCC	4 cut(s)	163, 682, 1126, 1154
PspPPI	RGGWCCY	1 cut(s)	682
PsyI	GACNNNGTC	2 cut(s)	240, 818
PvuII	CAGCTG	2 cut(s)	1487, 1535
RsaI	GTAC	3 cut(s)	541, 803, 956
RsaNI	GTAC	3 cut(s)	540, 802, 955
RseI	CAYNNNNRTG	2 cut(s)	138, 764
SacI	GAGCTC	3 cut(s)	945, 1245, 1455
SaqAI	TTAA	2 cut(s)	1446, 1721
Sau3AI	GATC	9 cut(s)	136, 357, 411, 550, 673, 756, 1030, 1050, 1592
Sau96I	GGNCC	4 cut(s)	163, 682, 1126, 1154
SchI	GAGTC	1 cut(s)	760
ScrFI	CCNGG	3 cut(s)	1130, 1175, 1293
SduI	GDGCHC	4 cut(s)	881, 945, 1245, 1455
SfaNI	GCATC	5 cut(s)	342, 552, 900, 1314, 1519
SfcI	CTRYAG	2 cut(s)	687, 714
SfoI	GGCGCC	2 cut(s)	418, 1068
SfuI	TTCGAA	2 cut(s)	210, 1231
SgrAI	CRCCGGYG	1 cut(s)	1391
SinI	GGWCC	2 cut(s)	163, 682
SmiMI	CAYNNNNRTG	2 cut(s)	138, 764
SmlI	CTYRAG	1 cut(s)	1720
SmoI	CTYRAG	1 cut(s)	1720
SnaBI	TACGTA	1 cut(s)	517
SphI	GCATGC	1 cut(s)	1691
Sse9I	AATT	4 cut(s)	1320, 1460, 1631, 1692
SseBI	AGGCCT	1 cut(s)	479
SspDI	GGCGCC	2 cut(s)	416, 1066
SspMI	CTAG	3 cut(s)	1085, 1493, 1745
SstI	GAGCTC	3 cut(s)	945, 1245, 1455
StuI	AGGCCT	1 cut(s)	479
StyD4I	CCNGG	3 cut(s)	1128, 1173, 1291
TaaI	ACNGT	4 cut(s)	866, 1194, 1225, 1366
TaiI	ACGT	3 cut(s)	81, 519, 1401
TaqI	TCGA	6 cut(s)	210, 594, 1231, 1254, 1374, 1591
TaqII	GACCGA	1 cut(s)	249
TasI	AATT	4 cut(s)	1320, 1460, 1631, 1692
TatI	WGTACW	1 cut(s)	801
TauI	GCSGC	5 cut(s)	899, 974, 1067, 1072, 1221
TfiI	GAWTC	3 cut(s)	736, 790, 1009
Tru1I	TTAA	2 cut(s)	1446, 1721
Tru9I	TTAA	2 cut(s)	1446, 1721
TscAI	CASTG	1 cut(s)	871
TseFI	GTSAC	2 cut(s)	421, 586
TseI	GCWGC	5 cut(s)	112, 115, 835, 1532, 1559
Tsp45I	GTSAC	2 cut(s)	421, 586
TspDTI	ATGAA	5 cut(s)	385, 623, 1340, 1420, 1620
TspGWI	ACGGA	3 cut(s)	798, 1298, 1695
TspRI	CASTG	1 cut(s)	871
Tth111I	GACNNNGTC	2 cut(s)	240, 818
Vha464I	CTTAAG	1 cut(s)	1720
VpaK11BI	GGWCC	2 cut(s)	163, 682
XapI	RAATTY	2 cut(s)	1460, 1631
XceI	RCATGY	1 cut(s)	1691
XmiI	GTMKAC	2 cut(s)	519, 1677
XspI	CTAG	3 cut(s)	1085, 1493, 1745
ZraI	GACGTC	1 cut(s)	79

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.