MD08G1013000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013929
Physical Location & Seq
Reverse (-)
963044 .. 965517
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1013000.v1.1.491

Sequence Viewer

Length: 924 bp
ATGCCGCCGAAGGACGAGAAGCCCGCGAAGCCGCGGAGGGAGGTGGAAGCGGAAGAAGACGACGACGATGAGAGGAGCTTGGGTTCGATTGCGTTGGAGCGCAAGAAGAAGCAACCCACGAAAAGCAGTGGCAATGCGAATGGAACGGCGAAATCTACGGGCAGAGAAGCCGCTAAGGTGAAGAAGGAGGACAAGATGGAAGATTGGGATAAGGCCGCGAAGGCGAAGCCTAAACAAGAAGCCAGAGTGAAGAAGGAGGAGAACGGCGGCGGTAGCGACGAGGAATTGGGTTCGAAGCCGAGAAAGGGTTCGAATTCAAAGCCCCAAAAGGAAATCAAGAACAGCAAGGTGAAGAAAATGGAGGAGGAGGAGGAGGAGAAGAAGGGGAAGAAGAGGAAGGCGTCTGAGCCTGCGACGGAGGAGAAGAAGAAGAGGGAGAAGAAGGTGTATGATTTGCCTGGTCAGAGGAAGGACCCTCCCGAGAAGAAGGACCCACTTAGGCAATTCTACGAGTCGCTTCACCAGCAAATTCCGAGTAGCGAAATGGCGCAGTTCTGGATGATGGAATATGGTTTGCTACCCAAAGTGGAGGCAGAGAAAGTGTTTGAGAAGAAGCAGAAGAAGAGTCAACTGCAAAAGCTCACTTCTCCAACGAAATCCGTATCTTCTGTAAAGACGAGCACAAAGTCAGTTACTGTTAAGAAGAGCACAAAGTCAGTTACTGTTAAGAAGAGCACAATGTCAGTTACTGTTAAGAAGAACACACCATCACCCGCAGCGCCTTCAAATAAGAAGCCGACACCAATCTCCAAAAGCGCAACAAACCAGTCGAAGAAGCAGAAGACGGAAGATAGCAGCTCAGAGGGAGACTCTGATGATGACTTTGTGGCCAGAATGGTAAAGAAGAAGAAGCAAGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

34.39

Weight (kDa)

9.75

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19990
fragaria_vesca FvH4_2g38430
malus_domestica MD08G1013000.v1.1
prunus_persica Prupe.1G365000_v2.0.a1
pyrus_communis pycom08g01110 pycom15g01060
rosa_chinensis RchiOBHm_Chr6g0302881
rosa_laevigata RLG00000011128 RLG00000021252
rosa_multiflora Rmu_ssc0000460.1_g000012
rosa_roxburghii Rroxscaffold_7G00165200
rosa_rugosa Rorug06G0317900
rosa_samantha Rh6AG431300 Rh6BG471300 Rh6CG443700 Rh6DG430400
rosa_wichuraiana Rw6G037370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 26, 34, 218
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 2 cut(s) 313, 528
AcyI GRCGYC 1 cut(s) 401
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 2 cut(s) 318, 786
AjnI CCWGG 1 cut(s) 457
AloI GAACNNNNNNTCC 2 cut(s) 67, 99
AluBI AGCT 4 cut(s) 78, 640, 858, 920
AluI AGCT 4 cut(s) 78, 640, 858, 920
Alw21I GWGCWC 3 cut(s) 683, 710, 737
Alw26I GTCTC 1 cut(s) 861
AlwNI CAGNNNCTG 3 cut(s) 695, 722, 749
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 2 cut(s) 213, 888
ApeKI GCWGC 3 cut(s) 776, 855, 917
ApoI RAATTY 2 cut(s) 313, 528
Asp700I GAANNNNTTC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 102, 550, 781, 818
AspS9I GGNCC 2 cut(s) 472, 490
AsuHPI GGTGA 4 cut(s) 190, 361, 512, 762
AsuII TTCGAA 2 cut(s) 293, 311
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 2 cut(s) 472, 490
BalI TGGCCA 1 cut(s) 890
BbsI GAAGAC 2 cut(s) 63, 848
Bbv12I GWGCWC 3 cut(s) 683, 710, 737
BbvI GCAGC 2 cut(s) 788, 867
BccI CCATC 3 cut(s) 190, 556, 775
BceAI ACGGC 2 cut(s) 162, 280
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 861
BfoI RGCGCY 1 cut(s) 782
BglI GCCNNNNNGGC 1 cut(s) 221
BisI GCNGC 8 cut(s) 5, 32, 171, 216, 268, 777, 856, 918
BlsI GCNGC 8 cut(s) 6, 33, 172, 217, 269, 778, 857, 919
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 2 cut(s) 472, 490
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 2 cut(s) 472, 490
BmiI GGNNCC 2 cut(s) 474, 492
BmrFI CCNGG 1 cut(s) 459
BpiI GAAGAC 2 cut(s) 63, 848
BplI GAGNNNNNCTC 2 cut(s) 854, 886
Bpu10I CCTNAGC 1 cut(s) 174
Bpu14I TTCGAA 2 cut(s) 293, 311
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 826
Bse3DI GCAATG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 1 cut(s) 564
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 396, 873
BseNI ACTGG 1 cut(s) 826
BseRI GAGGAG 8 cut(s) 88, 272, 377, 380, 383, 386, 389, 434
BseXI GCAGC 2 cut(s) 788, 867
Bsh1236I CGCG 3 cut(s) 26, 34, 218
BshFI GGCC 2 cut(s) 215, 890
BsiHKAI GWGCWC 3 cut(s) 683, 710, 737
BsiHKCI CYCGRG 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 861
BsnI GGCC 2 cut(s) 215, 890
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 2 cut(s) 293, 311
Bsp1286I GDGCHC 3 cut(s) 683, 710, 737
BspANI GGCC 2 cut(s) 215, 890
BspCNI CTCAG 2 cut(s) 397, 872
BspFNI CGCG 3 cut(s) 26, 34, 218
BspLI GGNNCC 2 cut(s) 474, 492
BspQI GCTCTTC 2 cut(s) 698, 725
BspT104I TTCGAA 2 cut(s) 293, 311
BsrDI GCAATG 1 cut(s) 139
BsrI ACTGG 1 cut(s) 826
BssECI CCNNGG 1 cut(s) 32
BssNI GRCGYC 1 cut(s) 401
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 3 cut(s) 697, 724, 751
Bst6I CTCTTC 5 cut(s) 386, 425, 617, 698, 725
BstACI GRCGYC 1 cut(s) 401
BstBI TTCGAA 2 cut(s) 293, 311
BstC8I GCNNGC 3 cut(s) 24, 411, 915
BstDEI CTNAG 5 cut(s) 174, 405, 497, 859, 921
BstDSI CCRYGG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 564
BstFNI CGCG 3 cut(s) 26, 34, 218
BstH2I RGCGCY 1 cut(s) 782
BstHHI GCGC 4 cut(s) 102, 550, 781, 818
BstMAI GTCTC 1 cut(s) 861
BstMWI GCNNNNNNNGC 4 cut(s) 28, 221, 273, 523
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 457
BstUI CGCG 3 cut(s) 26, 34, 218
BstV1I GCAGC 2 cut(s) 788, 867
BstV2I GAAGAC 2 cut(s) 63, 848
BsuRI GGCC 2 cut(s) 215, 890
BtgI CCRYGG 1 cut(s) 32
BtsCI GGATG 1 cut(s) 564
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 3 cut(s) 24, 411, 915
CaiI CAGNNNCTG 3 cut(s) 695, 722, 749
CfoI GCGC 4 cut(s) 102, 550, 781, 818
Cfr13I GGNCC 2 cut(s) 472, 490
Cfr42I CCGCGG 1 cut(s) 35
CseI GACGC 1 cut(s) 390
DdeI CTNAG 5 cut(s) 174, 405, 497, 859, 921
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 5 cut(s) 386, 425, 617, 698, 725
EarI CTCTTC 5 cut(s) 386, 425, 617, 698, 725
Eco47I GGWCC 2 cut(s) 472, 490
Eco88I CYCGRG 1 cut(s) 479
EcoO109I RGGNCCY 2 cut(s) 472, 490
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 457
FaiI YATR 2 cut(s) 450, 570
FauI CCCGC 2 cut(s) 31, 781
Fnu4HI GCNGC 8 cut(s) 5, 32, 171, 216, 268, 777, 856, 918
FokI GGATG 1 cut(s) 571
Fsp4HI GCNGC 8 cut(s) 5, 32, 171, 216, 268, 777, 856, 918
GlaI GCGC 4 cut(s) 101, 549, 780, 817
GluI GCNGC 8 cut(s) 5, 32, 171, 216, 268, 777, 856, 918
HaeII RGCGCY 1 cut(s) 782
HaeIII GGCC 2 cut(s) 215, 890
HgaI GACGC 1 cut(s) 390
HhaI GCGC 4 cut(s) 102, 550, 781, 818
Hin1I GRCGYC 1 cut(s) 401
Hin6I GCGC 4 cut(s) 100, 548, 779, 816
HinP1I GCGC 4 cut(s) 100, 548, 779, 816
HincII GTYRAC 1 cut(s) 629
HindII GTYRAC 1 cut(s) 629
HinfI GANTC 3 cut(s) 512, 625, 869
HphI GGTGA 4 cut(s) 190, 361, 512, 762
Hpy166II GTNNAC 1 cut(s) 629
Hpy188I TCNGA 5 cut(s) 406, 465, 534, 862, 874
Hpy188III TCNNGA 3 cut(s) 337, 479, 556
Hpy8I GTNNAC 1 cut(s) 629
Hpy99I CGWCG 4 cut(s) 65, 68, 281, 418
HpyCH4III ACNGT 3 cut(s) 697, 724, 751
HpyCH4V TGCA 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 221, 273, 523
HpyF3I CTNAG 5 cut(s) 174, 405, 497, 859, 921
Hsp92I GRCGYC 1 cut(s) 401
HspAI GCGC 4 cut(s) 100, 548, 779, 816
KspI CCGCGG 1 cut(s) 35
LguI GCTCTTC 2 cut(s) 698, 725
LmnI GCTCC 2 cut(s) 75, 97
LpnPI CCDG 8 cut(s) 256, 423, 444, 471, 536, 541, 839, 904
Lsp1109I GCAGC 2 cut(s) 788, 867
MaeIII GTNAC 3 cut(s) 691, 718, 745
MhlI GDGCHC 3 cut(s) 683, 710, 737
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 4 cut(s) 284, 313, 503, 528
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 3 cut(s) 521, 634, 863
MmeI TCCRAC 2 cut(s) 75, 674
Mox20I TGGCCA 1 cut(s) 890
MroXI GAANNNNTTC 1 cut(s) 307
MscI TGGCCA 1 cut(s) 890
MseI TTAA 3 cut(s) 699, 726, 753
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 3 cut(s) 26, 34, 218
MwoI GCNNNNNNNGC 4 cut(s) 28, 221, 273, 523
NlaIV GGNNCC 2 cut(s) 474, 492
NmeAIII GCCGAG 1 cut(s) 324
NspV TTCGAA 2 cut(s) 293, 311
PciSI GCTCTTC 2 cut(s) 698, 725
PdmI GAANNNNTTC 1 cut(s) 307
PkrI GCNGC 8 cut(s) 6, 33, 172, 217, 269, 778, 857, 919
PleI GAGTC 3 cut(s) 520, 633, 863
PpsI GAGTC 3 cut(s) 520, 633, 863
PpuMI RGGWCCY 2 cut(s) 472, 490
Psp5II RGGWCCY 2 cut(s) 472, 490
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 474, 492
PspPI GGNCC 2 cut(s) 472, 490
PspPPI RGGWCCY 2 cut(s) 472, 490
PstNI CAGNNNCTG 3 cut(s) 695, 722, 749
SacII CCGCGG 1 cut(s) 35
SapI GCTCTTC 2 cut(s) 698, 725
SaqAI TTAA 3 cut(s) 699, 726, 753
SatI GCNGC 8 cut(s) 5, 32, 171, 216, 268, 777, 856, 918
Sau96I GGNCC 2 cut(s) 472, 490
SchI GAGTC 3 cut(s) 521, 634, 863
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 3 cut(s) 683, 710, 737
SetI ASST 8 cut(s) 45, 80, 180, 351, 447, 642, 860, 922
Sfr303I CCGCGG 1 cut(s) 35
SfuI TTCGAA 2 cut(s) 293, 311
SgrBI CCGCGG 1 cut(s) 35
SinI GGWCC 2 cut(s) 472, 490
Sse9I AATT 4 cut(s) 284, 313, 503, 528
StyD4I CCNGG 1 cut(s) 457
TaaI ACNGT 3 cut(s) 697, 724, 751
TaqI TCGA 4 cut(s) 86, 293, 311, 830
TasI AATT 4 cut(s) 284, 313, 503, 528
TauI GCSGC 5 cut(s) 7, 34, 173, 218, 270
Tru1I TTAA 3 cut(s) 699, 726, 753
Tru9I TTAA 3 cut(s) 699, 726, 753
TscAI CASTG 1 cut(s) 133
TseI GCWGC 3 cut(s) 776, 855, 917
TspGWI ACGGA 3 cut(s) 431, 649, 860
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 472, 490
XapI RAATTY 2 cut(s) 313, 528
XmnI GAANNNNTTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.