Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010080

Physical Location & Seq

Genomic Coordinates Forward (+)

1219027 .. 1224063

Gene Structure 5037 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1015500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGATGGGTTCGGTTGAGGAGAAGCTCAAAACTGGATGCTTGCTTGATGAGATGAAGCTGCTGAAAGAGCTGCAGGATCACTCTGGGTCCCGGAAGGCTATAAATTCGGAGCTGTGGCACGCCTGTGCCGGCCCGCTTGTTTGTTTGCCGCAGGTTGGGAGTCTCTCGTACTACTTCCCCCAAGGACATAGCGAACAGGTGGCGGTTTCGACGAAAAGAACAGCAACCTCACAAATCCCCAACTACCCGAATCTCCCGTCTCAATTGCTATGTCAAGTTCAAAATGTTACACTGCATGCAGACAAAGAAACTGACGAAATCTATGCACAAATGATCCTTAAACCAGTGAATTCTGAAAAGGATGTCTTCCCGGTATCAGACTTTAGAATGAAGCCTAGCAAGCATCCATCGGAGTTTTTCTGCAAAACTTTGACTGCAAGTGACACAAGCACGCATGGGGGCTTCTCCGTGCCGCGTAGGGCAGCAGAAAAGCTCTTTCCTCCGCTGGATTTCACAATGCAACCACCAACCCAAGAACTTGTTGTCCGAGACTTGCACGACAATACCTGGACATTTCGCCACATTTATCGCGGGCAGCCGAAACGACACCTTCTCACAACTGGATGGAGTGTGTTTGTTGGTGCAAAGAGGCTTAGAGCAGGCGATTCCGTTCTATTTATCAGGGATGAGAAGTCACAGCTCTTGATTGGTGTGAGACGAGCAAATCGTCAGCAGACGACTTTGCCGTCATCAGTTTTATCTGCTGATAGCATGCACATTGGCGTCCTTGCTGCTGCGGCTCATGCTGCATCTAATCGAAGTTCATTCACTATATTCTACAATCCAAGGGCATGCCCTTCAGAATTTGTCATACCTCTGGCTACATTCCAGAAGGCTGTATATGGGACACAACTCTCAATTGGCATGAGGTTTGGAATGATGTTCGAGACAGAGGAGTCGGGCAAGCGCAGATATATGGGTACGATAGTTGGTATCAGTGATTTAGATCCGCTGAGATGGCCTGGTTCGAAGTGGCGAAATCTTCAGGTAGAGTGGGATGAGCCAGGGTGTTGTGATAAACAGAACAGGGTTAGTTCATGGGAAATCGAGACTCCGGAAAGTCTTTTCATATTTCCTTCTCTGACTTCAAGTCTTAAAAGACCGATGCACTCCGGATTCTTGGGAGCAGAAACTGAGTGGGGAAATATGATCAAGAGACCTTTCATCCGGGTTCCTGAGATTGGAAACGGGAACTCTTATCCATACCCGATTTCAAATCTATGTTCAGAACAACTAGTCAATATGCTAATGAAACCTCAACTTGCTAATCATGCTGGAACATTTGCTGCTCTACAACGGGAATCGGCCTCTAATGCAGATTCAGTAGTAGATATGAAGGCCATGTATGCCAAAATCAACCAAAAGAATCTAGCTGATGGCGTAAACGCAAATGCACCATCTCATGAACTTCCACCAGGAAGGTTGAACGTGGCAGCTAAGTTTGGAAGCCAAACTCCAGTTGGAAACGGCACTGAGAAGACGAAATTAGAACCTGAATTTTCCGCGGATCAGTTAAGCCAGTTGAATTCTGTAGGACATGGCATTGAGGATAAATTAGCTACTGGGTTCGCGAGTCCTTATAGCCATGTGAACCAGCTTACATTTGCCAACCAAAACCAAGATGCAGCGCAACTACAAACTAGCCCGCGGCCTATGCAGCCACCTTTGGAATCACTACTCTTCCACTCACAAACGGATCTACCTCATTCGGATTTCAATGGCATGAACAGTTCACTCCCATTCCTAGACAATGATGAATGCATGTTTTACTCATCTTGCCAATCCTTTTCCGGGGCATTGAGATCACCGGGGCCTTTGTCTGCATTCGGTTTACAAGAGTCTTCAGCTGTTTTAACTGAAGCAAATAATTCCTCCCTAACTTCAATCGGTCAGGACATGTGGGATAATACCCTCATTAATTCAAGCAGCTTGAGAGATTTGTCTGATGAGAGCAACAATCAAAGTGGGATCTATGGCTGTCCAAGCATTGATGTTGGTAGCTGCTTAAGTACTGTCGTCGACCCTTCTGTTTCAAGCACCATACTCAATGACTTTTCTACGCTGAAGAACTCCAACTTCCAGAATTCGTCGGATTGTTTGGTCAGAAACTTGAGCTCAAGCCAGGATCTTCAGTCGCAGATTACCTCGGCAAGCCTGGGAGACTCTCAGGCTTTCTCCAGGCAAGACCTGGCAGACAACTCTGGCGGTACATCCTCAAGCAATGTAGATCTCGATGAGAGCAGTCTGCTGAAGAACAGTTCGTGGCATAAAGTGGTTGCGCCCGTGCGAACCTACACGAAGGTTCAAAAGACGGGATCCGTTGGGAGGTCGATCGACGTTACGAATTTCAAAAACTACGAAGAACTATGCTCTGCAATTGAATGCATGTTTGGACTGAAAGGGCTGCTAAACGATCCAAGAGGTTCAGGGTGGAAATTGGTTTATGTGGATTATGAGAACGACGTTCTACTTGTCGGGGATGATCCTTGGGAGGAATTCGTTGGTTGTGTTCGCTGCATCAGAATCCTTTCGCCTACGGAAGTTCAGCAGATGAGCGAAGAGGGCATGAAGCTTCTGAACAGCGCTGCGATGCAAGGGATGAACGGCACCATGTCCGAAGTTGGGCGTCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001067 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009889 GO:0009908 GO:0009942 GO:0010033 GO:0010051 GO:0010154 GO:0010305 GO:0010468 GO:0010556 GO:0016020 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

898

Amino Acids

98.63

Weight (kDa)

5.64

Isoelectric Point (pI)

52.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	140 - 241	3.7e-22	B3 DNA binding domain
ARF_AD	PF06507	289 - 371	6.3e-33	Auxin response factor ancillary domain
AUX_IAA	PF02309	828 - 869	8.8e-07	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19850 AT1G19850
fragaria_vesca	FvH4_2g38760 FvH4_2g38760 FvH4_2g38760
malus_domestica	MD08G1015500.v1.1 MD15G1014400.v1.1
prunus_persica	Prupe.1G368300_v2.0.a1
pyrus_communis	pycom08g01360 pycom15g01250
rosa_chinensis	RchiOBHm_Chr6g0302551
rosa_laevigata	RLG00000011162
rosa_multiflora	Rmu_sc0007717.1_g000001
rosa_roxburghii	Rroxscaffold_7G00165620
rosa_rugosa	Rorug06G0315000 Rorug06G0315000
rosa_samantha	Rh6AG428300 Rh6BG474400 Rh6CG440400 Rh6DG427400
rosa_wichuraiana	Rw6G037050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	745, 955
Acc36I	ACCTGC	1 cut(s)	142
AccB1I	GGYRCC	1 cut(s)	2666
AccI	GTMKAC	1 cut(s)	2079
AccII	CGCG	5 cut(s)	475, 591, 1565, 1631, 1708
AccIII	TCCGGA	2 cut(s)	1114, 1172
AcsI	RAATTY	8 cut(s)	103, 349, 863, 1556, 1585, 2143, 2404, 2555
AcuI	CTGAAG	7 cut(s)	843, 1028, 1887, 1938, 2144, 2174, 2330
AcyI	GRCGYC	2 cut(s)	783, 2686
AfaI	GTAC	4 cut(s)	170, 982, 2071, 2269
AfeI	AGCGCT	1 cut(s)	2644
AfiI	CCNNNNNNNGG	5 cut(s)	155, 1241, 1851, 2385, 2682
AflII	CTTAAG	1 cut(s)	2065
AflIII	ACRYGT	1 cut(s)	1956
AhdI	GACNNNNNGTC	1 cut(s)	1149
AhlI	ACTAGT	1 cut(s)	1294
AjnI	CCWGG	8 cut(s)	566, 1021, 1063, 1474, 2181, 2214, 2237, 2247
AjuI	GAANNNNNNNTTGG	2 cut(s)	2433, 2465
AleI	CACNNNNGTG	1 cut(s)	123
AloI	GAACNNNNNNTCC	2 cut(s)	528, 560
Alw21I	GWGCWC	1 cut(s)	2177
Alw26I	GTCTC	8 cut(s)	167, 264, 543, 709, 941, 1103, 1210, 2214
AlwNI	CAGNNNCTG	1 cut(s)	1193
Aor13HI	TCCGGA	2 cut(s)	1114, 1172
Aor51HI	AGCGCT	1 cut(s)	2644
AoxI	GGCC	6 cut(s)	130, 1019, 1365, 1398, 1709, 1871
ApoI	RAATTY	8 cut(s)	103, 349, 863, 1556, 1585, 2143, 2404, 2555
ArsI	GACNNNNNNTTYG	2 cut(s)	242, 274
AseI	ATTAAT	1 cut(s)	1977
Asp700I	GAANNNNTTC	1 cut(s)	2587
AspLEI	GCGC	4 cut(s)	969, 1690, 2341, 2645
AspS9I	GGNCC	3 cut(s)	87, 131, 1871
AsuC2I	CCSGG	5 cut(s)	91, 371, 1229, 1852, 1869
AsuHPI	GGTGA	1 cut(s)	1857
AsuII	TTCGAA	1 cut(s)	1028
AvaII	GGWCC	1 cut(s)	87
BaeI	ACNNNNGTAYC	2 cut(s)	972, 1005
BamHI	GGATCC	1 cut(s)	2375
BanI	GGYRCC	1 cut(s)	2666
BanII	GRGCYC	1 cut(s)	2177
BbsI	GAAGAC	3 cut(s)	358, 1544, 1893
Bbv12I	GWGCWC	1 cut(s)	2177
BccI	CCATC	5 cut(s)	415, 618, 1011, 1430, 1465
BceAI	ACGGC	3 cut(s)	730, 1543, 2680
BcgI	CGANNNNNNTGC	2 cut(s)	305, 339
BciT130I	CCWGG	8 cut(s)	568, 1023, 1065, 1476, 2183, 2216, 2239, 2249
BclI	TGATCA	1 cut(s)	1209
BcnI	CCSGG	5 cut(s)	91, 371, 1229, 1852, 1869
BcoDI	GTCTC	8 cut(s)	167, 264, 543, 709, 941, 1103, 1210, 2214
BcuI	ACTAGT	1 cut(s)	1294
BfaI	CTAG	5 cut(s)	396, 1295, 1430, 1701, 1805
BfmI	CTRYAG	2 cut(s)	71, 1590
BfoI	RGCGCY	1 cut(s)	2646
BfrI	CTTAAG	1 cut(s)	2065
BfuAI	ACCTGC	1 cut(s)	142
BglII	AGATCT	1 cut(s)	2287
BmcAI	AGTACT	1 cut(s)	2071
Bme18I	GGWCC	1 cut(s)	87
BmeRI	GACNNNNNGTC	1 cut(s)	1149
BmgT120I	GGNCC	3 cut(s)	87, 131, 1871
BmiI	GGNNCC	6 cut(s)	88, 89, 1233, 1872, 2377, 2668
BmrI	ACTGGG	1 cut(s)	1632
BmsI	GCATC	7 cut(s)	26, 412, 818, 1155, 1672, 2586, 2640
BmuI	ACTGGG	1 cut(s)	1632
BpiI	GAAGAC	3 cut(s)	358, 1544, 1893
BpmI	CTGGAG	2 cut(s)	1500, 2221
Bpu14I	TTCGAA	1 cut(s)	1028
BpuEI	CTTGAG	4 cut(s)	2011, 2161, 2191, 2260
BpuMI	CCSGG	5 cut(s)	91, 371, 1229, 1852, 1869
BsaBI	GATNNNNATC	1 cut(s)	1005
BsaHI	GRCGYC	2 cut(s)	783, 2686
BsaI	GGTCTC	1 cut(s)	1210
BsaWI	WCCGGW	2 cut(s)	1114, 1172
Bsc4I	CCNNNNNNNGG	5 cut(s)	155, 1241, 1851, 2385, 2682
Bse118I	RCCGGY	1 cut(s)	128
Bse1I	ACTGG	6 cut(s)	37, 344, 625, 1517, 1579, 1627
Bse3DI	GCAATG	1 cut(s)	2287
Bse8I	GATNNNNATC	1 cut(s)	1005
BseAI	TCCGGA	2 cut(s)	1114, 1172
BseBI	CCWGG	8 cut(s)	568, 1023, 1065, 1476, 2183, 2216, 2239, 2249
BseJI	GATNNNNATC	1 cut(s)	1005
BseLI	CCNNNNNNNGG	5 cut(s)	155, 1241, 1851, 2385, 2682
BseMI	GCAATG	1 cut(s)	2287
BseMII	CTCAG	5 cut(s)	1004, 1185, 1227, 1524, 2240
BseNI	ACTGG	6 cut(s)	37, 344, 625, 1517, 1579, 1627
BseRI	GAGGAG	2 cut(s)	32, 968
Bsh1236I	CGCG	5 cut(s)	475, 591, 1565, 1631, 1708
Bsh1285I	CGRYCG	1 cut(s)	2394
BshFI	GGCC	6 cut(s)	132, 1021, 1367, 1400, 1711, 1873
BshNI	GGYRCC	1 cut(s)	2666
BsiEI	CGRYCG	1 cut(s)	2394
BsiHKAI	GWGCWC	1 cut(s)	2177
BsiSI	CCGG	8 cut(s)	91, 129, 371, 1115, 1173, 1228, 1851, 1868
BslFI	GGGAC	2 cut(s)	73, 919
BslI	CCNNNNNNNGG	5 cut(s)	155, 1241, 1851, 2385, 2682
BsmAI	GTCTC	8 cut(s)	167, 264, 543, 709, 941, 1103, 1210, 2214
BsmBI	CGTCTC	2 cut(s)	264, 709
BsmFI	GGGAC	2 cut(s)	73, 919
BsmI	GAATGC	3 cut(s)	1823, 1883, 2447
BsnI	GGCC	6 cut(s)	132, 1021, 1367, 1400, 1711, 1873
Bso31I	GGTCTC	1 cut(s)	1210
Bsp119I	TTCGAA	1 cut(s)	1028
Bsp1286I	GDGCHC	1 cut(s)	2177
Bsp13I	TCCGGA	2 cut(s)	1114, 1172
Bsp68I	TCGCGA	1 cut(s)	1631
BspANI	GGCC	6 cut(s)	132, 1021, 1367, 1400, 1711, 1873
BspCNI	CTCAG	5 cut(s)	1005, 1186, 1228, 1525, 2239
BspEI	TCCGGA	2 cut(s)	1114, 1172
BspFNI	CGCG	5 cut(s)	475, 591, 1565, 1631, 1708
BspHI	TCATGA	1 cut(s)	1462
BspLI	GGNNCC	6 cut(s)	88, 89, 1233, 1872, 2377, 2668
BspMAI	CTGCAG	1 cut(s)	75
BspMI	ACCTGC	1 cut(s)	142
BspT104I	TTCGAA	1 cut(s)	1028
BspT107I	GGYRCC	1 cut(s)	2666
BspTI	CTTAAG	1 cut(s)	2065
BspTNI	GGTCTC	1 cut(s)	1210
BsrDI	GCAATG	1 cut(s)	2287
BsrFI	RCCGGY	1 cut(s)	128
BsrI	ACTGG	6 cut(s)	37, 344, 625, 1517, 1579, 1627
BssAI	RCCGGY	1 cut(s)	128
BssNI	GRCGYC	2 cut(s)	783, 2686
BssT1I	CCWWGG	3 cut(s)	181, 845, 2546
Bst2UI	CCWGG	8 cut(s)	568, 1023, 1065, 1476, 2183, 2216, 2239, 2249
Bst4CI	ACNGT	3 cut(s)	1790, 2074, 2318
Bst6I	CTCTTC	2 cut(s)	1745, 2613
BstACI	GRCGYC	2 cut(s)	783, 2686
BstAFI	CTTAAG	1 cut(s)	2065
BstBI	TTCGAA	1 cut(s)	1028
BstDEI	CTNAG	7 cut(s)	653, 1013, 1194, 1236, 1497, 1533, 2226
BstDSI	CCRYGG	2 cut(s)	1563, 1706
BstFNI	CGCG	5 cut(s)	475, 591, 1565, 1631, 1708
BstH2I	RGCGCY	1 cut(s)	2646
BstHHI	GCGC	4 cut(s)	969, 1690, 2341, 2645
BstMAI	GTCTC	8 cut(s)	167, 264, 543, 709, 941, 1103, 1210, 2214
BstMCI	CGRYCG	1 cut(s)	2394
BstNI	CCWGG	8 cut(s)	568, 1023, 1065, 1476, 2183, 2216, 2239, 2249
BstNSI	RCATGY	6 cut(s)	299, 775, 855, 1825, 1960, 2449
BstSFI	CTRYAG	2 cut(s)	71, 1590
BstUI	CGCG	5 cut(s)	475, 591, 1565, 1631, 1708
BstV2I	GAAGAC	3 cut(s)	358, 1544, 1893
BstX2I	RGATCY	6 cut(s)	1006, 1756, 2028, 2185, 2287, 2375
BstYI	RGATCY	6 cut(s)	1006, 1756, 2028, 2185, 2287, 2375
BsuRI	GGCC	6 cut(s)	132, 1021, 1367, 1400, 1711, 1873
BtgI	CCRYGG	2 cut(s)	1563, 1706
BtgZI	GCGATG	1 cut(s)	2663
BtsI	GCAGTG	1 cut(s)	290
BtsIMutI	CAGTG	4 cut(s)	290, 351, 1003, 1530
BtuMI	TCGCGA	1 cut(s)	1631
BveI	ACCTGC	1 cut(s)	142
CaiI	CAGNNNCTG	1 cut(s)	1193
CciI	TCATGA	1 cut(s)	1462
CfoI	GCGC	4 cut(s)	969, 1690, 2341, 2645
Cfr10I	RCCGGY	1 cut(s)	128
Cfr13I	GGNCC	3 cut(s)	87, 131, 1871
Cfr42I	CCGCGG	2 cut(s)	1566, 1709
CseI	GACGC	2 cut(s)	772, 2675
Csp6I	GTAC	4 cut(s)	169, 981, 2070, 2268
CspCI	CAANNNNNGTGG	2 cut(s)	2005, 2040
CviQI	GTAC	4 cut(s)	169, 981, 2070, 2268
DdeI	CTNAG	7 cut(s)	653, 1013, 1194, 1236, 1497, 1533, 2226
DrdI	GACNNNNNNGTC	2 cut(s)	745, 955
DriI	GACNNNNNGTC	1 cut(s)	1149
DseDI	GACNNNNNNGTC	2 cut(s)	745, 955
Eam1104I	CTCTTC	2 cut(s)	1745, 2613
Eam1105I	GACNNNNNGTC	1 cut(s)	1149
EarI	CTCTTC	2 cut(s)	1745, 2613
Ecl136II	GAGCTC	1 cut(s)	2175
Eco130I	CCWWGG	3 cut(s)	181, 845, 2546
Eco24I	GRGCYC	1 cut(s)	2177
Eco31I	GGTCTC	1 cut(s)	1210
Eco47I	GGWCC	1 cut(s)	87
Eco47III	AGCGCT	1 cut(s)	2644
Eco53kI	GAGCTC	1 cut(s)	2175
Eco57I	CTGAAG	7 cut(s)	843, 1028, 1887, 1938, 2144, 2174, 2330
EcoICRI	GAGCTC	1 cut(s)	2175
EcoO109I	RGGNCCY	2 cut(s)	87, 1871
EcoRI	GAATTC	4 cut(s)	349, 1585, 2143, 2555
EcoRII	CCWGG	8 cut(s)	566, 1021, 1063, 1474, 2181, 2214, 2237, 2247
EcoT14I	CCWWGG	3 cut(s)	181, 845, 2546
EcoT22I	ATGCAT	2 cut(s)	1823, 2447
EcoT38I	GRGCYC	1 cut(s)	2177
ErhI	CCWWGG	3 cut(s)	181, 845, 2546
Esp3I	CGTCTC	2 cut(s)	264, 709
FalI	AAGNNNNNCTT	2 cut(s)	350, 382
FaqI	GGGAC	2 cut(s)	73, 919
FauI	CCCGC	3 cut(s)	141, 584, 1713
FbaI	TGATCA	1 cut(s)	1209
FblI	GTMKAC	1 cut(s)	2079
FriOI	GRGCYC	1 cut(s)	2177
FspBI	CTAG	5 cut(s)	396, 1295, 1430, 1701, 1805
GlaI	GCGC	4 cut(s)	968, 1689, 2340, 2644
GsuI	CTGGAG	2 cut(s)	1500, 2221
HaeII	RGCGCY	1 cut(s)	2646
HaeIII	GGCC	6 cut(s)	132, 1021, 1367, 1400, 1711, 1873
HapII	CCGG	8 cut(s)	91, 129, 371, 1115, 1173, 1228, 1851, 1868
HgaI	GACGC	2 cut(s)	772, 2675
HhaI	GCGC	4 cut(s)	969, 1690, 2341, 2645
Hin1I	GRCGYC	2 cut(s)	783, 2686
Hin6I	GCGC	4 cut(s)	967, 1688, 2339, 2643
HinP1I	GCGC	4 cut(s)	967, 1688, 2339, 2643
HincII	GTYRAC	1 cut(s)	2080
HindII	GTYRAC	1 cut(s)	2080
HindIII	AAGCTT	1 cut(s)	2630
HpaII	CCGG	8 cut(s)	91, 129, 371, 1115, 1173, 1228, 1851, 1868
HphI	GGTGA	1 cut(s)	1857
Hpy166II	GTNNAC	5 cut(s)	1444, 1651, 1793, 1892, 2080
Hpy8I	GTNNAC	5 cut(s)	1444, 1651, 1793, 1892, 2080
Hpy99I	CGWCG	5 cut(s)	214, 2081, 2152, 2399, 2525
HpyAV	CCTTC	9 cut(s)	88, 620, 867, 886, 1146, 1390, 1473, 2094, 2353
HpyCH4III	ACNGT	3 cut(s)	1790, 2074, 2318
HpyCH4IV	ACGT	3 cut(s)	1488, 2397, 2523
HpyF3I	CTNAG	7 cut(s)	653, 1013, 1194, 1236, 1497, 1533, 2226
HpySE526I	ACGT	3 cut(s)	1488, 2397, 2523
Hsp92I	GRCGYC	2 cut(s)	783, 2686
HspAI	GCGC	4 cut(s)	967, 1688, 2339, 2643
KflI	GGGWCCC	1 cut(s)	87
Kpn2I	TCCGGA	2 cut(s)	1114, 1172
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
Ksp22I	TGATCA	1 cut(s)	1209
KspI	CCGCGG	2 cut(s)	1566, 1709
LmnI	GCTCC	2 cut(s)	109, 1184
LweI	GCATC	7 cut(s)	26, 412, 818, 1155, 1672, 2586, 2640
MaeI	CTAG	5 cut(s)	396, 1295, 1430, 1701, 1805
MaeII	ACGT	3 cut(s)	1488, 2397, 2523
MaeIII	GTNAC	4 cut(s)	286, 440, 693, 2398
MfeI	CAATTG	3 cut(s)	263, 918, 2436
MflI	RGATCY	6 cut(s)	1006, 1756, 2028, 2185, 2287, 2375
MhlI	GDGCHC	1 cut(s)	2177
MlyI	GAGTC	6 cut(s)	169, 965, 1105, 1642, 1907, 2216
MmeI	TCCRAC	3 cut(s)	1501, 2130, 2157
Mph1103I	ATGCAT	2 cut(s)	1823, 2447
MroI	TCCGGA	2 cut(s)	1114, 1172
MroNI	GCCGGC	1 cut(s)	128
MroXI	GAANNNNTTC	1 cut(s)	2587
MseI	TTAA	7 cut(s)	339, 1155, 1574, 1913, 1977, 2066, 2692
MslI	CAYNNNNRTG	1 cut(s)	123
MspA1I	CMGCKG	5 cut(s)	505, 1012, 1565, 1708, 1907
MspCI	CTTAAG	1 cut(s)	2065
MspI	CCGG	8 cut(s)	91, 129, 371, 1115, 1173, 1228, 1851, 1868
MunI	CAATTG	3 cut(s)	263, 918, 2436
Mva1269I	GAATGC	3 cut(s)	1823, 1883, 2447
MvaI	CCWGG	8 cut(s)	568, 1023, 1065, 1476, 2183, 2216, 2239, 2249
MvnI	CGCG	5 cut(s)	475, 591, 1565, 1631, 1708
NaeI	GCCGGC	1 cut(s)	130
NciI	CCSGG	5 cut(s)	91, 371, 1229, 1852, 1869
NgoMIV	GCCGGC	1 cut(s)	128
NlaIV	GGNNCC	6 cut(s)	88, 89, 1233, 1872, 2377, 2668
NmeAIII	GCCGAG	1 cut(s)	2186
NmuCI	GTSAC	2 cut(s)	440, 693
NruI	TCGCGA	1 cut(s)	1631
NsiI	ATGCAT	2 cut(s)	1823, 2447
NspI	RCATGY	6 cut(s)	299, 775, 855, 1825, 1960, 2449
NspV	TTCGAA	1 cut(s)	1028
OliI	CACNNNNGTG	1 cut(s)	123
PaeI	GCATGC	3 cut(s)	299, 775, 855
PagI	TCATGA	1 cut(s)	1462
PciI	ACATGT	1 cut(s)	1956
PcsI	WCGNNNNNNNCGW	2 cut(s)	724, 743
PctI	GAATGC	3 cut(s)	1823, 1883, 2447
PdiI	GCCGGC	1 cut(s)	130
PdmI	GAANNNNTTC	1 cut(s)	2587
PfeI	GAWTC	8 cut(s)	250, 665, 1176, 1361, 1379, 1426, 1730, 2583
PfoI	TCCNGGA	1 cut(s)	89
Ple19I	CGATCG	1 cut(s)	2394
PleI	GAGTC	6 cut(s)	168, 964, 1105, 1641, 1906, 2216
PpsI	GAGTC	6 cut(s)	168, 964, 1105, 1641, 1906, 2216
PpuMI	RGGWCCY	1 cut(s)	87
PscI	ACATGT	1 cut(s)	1956
PshBI	ATTAAT	1 cut(s)	1977
Psp124BI	GAGCTC	1 cut(s)	2177
Psp5II	RGGWCCY	1 cut(s)	87
Psp6I	CCWGG	8 cut(s)	566, 1021, 1063, 1474, 2181, 2214, 2237, 2247
PspGI	CCWGG	8 cut(s)	566, 1021, 1063, 1474, 2181, 2214, 2237, 2247
PspN4I	GGNNCC	6 cut(s)	88, 89, 1233, 1872, 2377, 2668
PspPI	GGNCC	3 cut(s)	87, 131, 1871
PspPPI	RGGWCCY	1 cut(s)	87
PstI	CTGCAG	1 cut(s)	75
PstNI	CAGNNNCTG	1 cut(s)	1193
PsuI	RGATCY	6 cut(s)	1006, 1756, 2028, 2185, 2287, 2375
PvuI	CGATCG	1 cut(s)	2394
PvuII	CAGCTG	1 cut(s)	1907
RruI	TCGCGA	1 cut(s)	1631
RsaI	GTAC	4 cut(s)	170, 982, 2071, 2269
RsaNI	GTAC	4 cut(s)	169, 981, 2070, 2268
RseI	CAYNNNNRTG	1 cut(s)	123
SacI	GAGCTC	1 cut(s)	2177
SacII	CCGCGG	2 cut(s)	1566, 1709
SalI	GTCGAC	1 cut(s)	2078
SaqAI	TTAA	7 cut(s)	339, 1155, 1574, 1913, 1977, 2066, 2692
Sau96I	GGNCC	3 cut(s)	87, 131, 1871
ScaI	AGTACT	1 cut(s)	2071
SchI	GAGTC	6 cut(s)	169, 965, 1105, 1642, 1907, 2216
SduI	GDGCHC	1 cut(s)	2177
SfaNI	GCATC	7 cut(s)	26, 412, 818, 1155, 1672, 2586, 2640
SfcI	CTRYAG	2 cut(s)	71, 1590
Sfr303I	CCGCGG	2 cut(s)	1566, 1709
SfuI	TTCGAA	1 cut(s)	1028
SgrBI	CCGCGG	2 cut(s)	1566, 1709
SinI	GGWCC	1 cut(s)	87
SmiMI	CAYNNNNRTG	1 cut(s)	123
SmlI	CTYRAG	5 cut(s)	1990, 2065, 2170, 2176, 2275
SmoI	CTYRAG	5 cut(s)	1990, 2065, 2170, 2176, 2275
SpeI	ACTAGT	1 cut(s)	1294
SphI	GCATGC	3 cut(s)	299, 775, 855
SspMI	CTAG	5 cut(s)	396, 1295, 1430, 1701, 1805
SstI	GAGCTC	1 cut(s)	2177
StyI	CCWWGG	3 cut(s)	181, 845, 2546
TaaI	ACNGT	3 cut(s)	1790, 2074, 2318
TaiI	ACGT	3 cut(s)	1491, 2400, 2526
TaqI	TCGA	9 cut(s)	209, 817, 945, 1028, 1107, 2079, 2292, 2390, 2394
TaqII	GACCGA	2 cut(s)	1177, 1937
TatI	WGTACW	1 cut(s)	2069
TauI	GCSGC	4 cut(s)	151, 475, 800, 1711
TfiI	GAWTC	8 cut(s)	250, 665, 1176, 1361, 1379, 1426, 1730, 2583
Tru1I	TTAA	7 cut(s)	339, 1155, 1574, 1913, 1977, 2066, 2692
Tru9I	TTAA	7 cut(s)	339, 1155, 1574, 1913, 1977, 2066, 2692
TscAI	CASTG	4 cut(s)	297, 351, 1003, 1537
TseFI	GTSAC	2 cut(s)	440, 693
Tsp45I	GTSAC	2 cut(s)	440, 693
TspGWI	ACGGA	5 cut(s)	457, 658, 1769, 2368, 2612
TspRI	CASTG	4 cut(s)	297, 351, 1003, 1537
Vha464I	CTTAAG	1 cut(s)	2065
VpaK11BI	GGWCC	1 cut(s)	87
VspI	ATTAAT	1 cut(s)	1977
XapI	RAATTY	8 cut(s)	103, 349, 863, 1556, 1585, 2143, 2404, 2555
XceI	RCATGY	6 cut(s)	299, 775, 855, 1825, 1960, 2449
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1517, 2245
XmiI	GTMKAC	1 cut(s)	2079
XmnI	GAANNNNTTC	1 cut(s)	2587
XspI	CTAG	5 cut(s)	396, 1295, 1430, 1701, 1805
ZrmI	AGTACT	1 cut(s)	2071
Zsp2I	ATGCAT	2 cut(s)	1823, 2447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.