Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010080

Physical Location & Seq

Genomic Coordinates Forward (+)

845596 .. 850392

Gene Structure 4797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1014400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGATGGGTTCGGTGGAGGAGAAGCTCAAAACTGGATGCTTGCTTGATGAGATGAAGCTGCTGAAAGAGCTGCAGGACCACTCTGGGACCCGGAAGGCTATAAATTCGGAGCTATGGCACGCTTGCGCCGGCCCGCTTGTTTGCTTGCCGCAGGTTGGGAGTCTCTCGTACTACTTCCCCCAAGGACATAGCGAACAGGTGGCGGTTTCGACGAAAAGAACGGCAACCTCACAAATTCCGAACTATCCCAATCTCCCTTCTCAGTTGCTATGCCAAGTTCAAAATGTTACACTGCATGCAGACAAAGAAACCGACGAAATCTATGCACAAATGAGCCTTAAACCAGTGAGCTCTGAAAGGGATGTCTTCCCGGTACCGGACTTTGGAATGAGGCCTAGTAAACATCCATCTGAGTTTTTCTGCAAAACTTTGACCGCAAGTGACACAAGCACACATGGAGGGTTCTCCGTGCCGCGTAGGGCAGCGGAAAAGCTCTTTCCTCCGCTGGATTTCACAATGCAGCCACCAACCCAAGAACTTGTTGTCCGAGACTTGCACGATAATACCTGGACATTTCGCCACATTTATCGCGGGCAGCCGAAACGACACCTTCTCACAACTGGATGGAGTTTGTTTGTTGGTGCAAAGAGGCTTAGAGCAGGGGATTCCGTTCTATTTATCAGGGATGAGAAGTCGCAGCTATTGATCGGTGTGAGACGAGCAAATCGTCAGCAAACAACATTGCCGTCATCAGTTCTATCTGCTGATAGCATGCACATTGGTGTCCTTGCTGCTGCGGCTCATGCTGCAGCTAATCGGAGTCCATTCACTATATTCTACAATCCAAGGGCATGCCCTTCAGAATTTGTCATACCTTTGGCCACCTTCCAAAAGGCTGTATTTGGGACACAGCTCTCGATTGGCATAAGGTTTGGAATGGTGTTCGAGACAGAGGAGTCGGGCAAGCGCAGATATATGGGCACGATAGTTGGTATTAGTGATTTAGATCCGCTGAGATGGCCTGGTTCAAAATGGCGAAATCTTCAGGTAGAGTGGGATGAGCCAGGGTGTTGTGATAAACAGAACAGGGTTAGTTCATGGGAAATCGAAACTCCGGAAAGTCTTTTCATATTTCCTTCTCTGACTTCGAGTCTTAAAAGACTGATGCACTCTGGATTCTTGGGAGCAGAAACCGAATGGGGAAATATGACCAAAAGATCATTCATCCGGGTTCCTGAGATTGGAAACGGGAACTCTTTTCCATACCCGATTTCAAATCTATGTTCGGAACAGCTAGTCAATATGCTAATGAAACCTCAGCTTGTTAATCATGCTGGAACATTAGCTACTCTACAACAGGAATCGGCTGCTAATGTAGATTCAGTAGATATGAAGGCCATGCAGGCCAAAATCAACCAGAAGAATCCAGCTGATGGCGTAAACGCGAATGCACCATCTCATGGAATTCCACCGGGAAATCTGAACACCGTAGCCAAGTTTGGAAGCCAAGCACCAGTTGGAAGTAGCACTGAGAAGATGAAATCAGAACCTGAACTTTCTGCAGATCAGTTAAGTCAGTTGAGTTCGGCAGGGCAAGGCATCGAGGATAAATTAGCTGCAGGGTTTGCGAGTCCCTATAACCATATGAATCAGCTGACATTTGCCAACCAAAACCCCAGTTCAGCGCAACTACAAACTAGTCCGCGGCCTATGCAGCCTTTGGATTCACTACTCTACCACTCTCAACAAACTGAATTACCTCATTCGGATTTCAATGGCATGAACAGTTCACTTCCATTCCTAGACAATGATGAATGCATGTTTTACTCTTCTTGCCTACCTTTTTCTGGGGCACTCAGATCACCGGGTCCTTTGTCTGCTTTCGGTTTACCAGATTCTTCAACTGTTTTAACTGACGCAAATACTTCCTCCCTAACTTCAATCGGTCAGGACATGTGGGATAATAGCCTCATTAATTCAAGCAGCCTGAGAGATTTGTCGGATGAGAGCAACAATCAAAGTGGGATCTACGGCTGTCCGAACATTGATGTTGGTAGCTGCTTAAGTACTGTCGTCGACCCTTCTGTCTCAAGCACTATACTCGATGACTTCTCTACGCTGAAGAATGCCAACTTCCAGAATTCTTCGGATTGTTTGGTTAAAAACTTGAGCTCAAGCCAGGATCTTCAGTCGCAGATTACCTCGGTGAGCCTTGGAGACTCTCAGGCTTTCTCTAGGCAAGACCTGGCAGACGCCTCAGGCGGTACATCCTCGAGCAATGTAGAACTTGATGAGAGCAGTCTACTGCAGAACAGTTCGTGGCATCCGGTGGTTGCACCTGTGCGAACCTACACAAAGGTTCAAAAGACGGGATCTGTTGGGCGGTCAATTGATGTTACTAACTTCAAAAACTACGAAGAACTATGCTCTGCAATCGAATGCATGTTCGGACTGGAAGGGCTGCTAAATGATCCAAGAGGTTCAGGGTGGAAATTGGTTTATGTAGATTATGAGAACGACGTTCTACTTGTCGGGGATGATCCTTGGGAGGAATTTGTTGGTTGTGTCCGCTGCATCAGAATCCTTTCGCCTACTGAAGTTCAGCAGATGAGCGAAGAAGGCATGAAGCTTCTGAACAGTGCTGCGATGCAAGGGATGAATAGCACCATGTCCGAAGGTGGCCGTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001067 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009889 GO:0009908 GO:0009942 GO:0010033 GO:0010051 GO:0010154 GO:0010305 GO:0010468 GO:0010556 GO:0016020 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

897

Amino Acids

98.04

Weight (kDa)

5.31

Isoelectric Point (pI)

56.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	140 - 241	5.9e-22	B3 DNA binding domain
ARF_AD	PF06507	289 - 371	3.1e-31	Auxin response factor ancillary domain
AUX_IAA	PF02309	780 - 868	1.5e-09	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19850 AT1G19850
fragaria_vesca	FvH4_2g38760 FvH4_2g38760 FvH4_2g38760
malus_domestica	MD08G1015500.v1.1 MD15G1014400.v1.1
prunus_persica	Prupe.1G368300_v2.0.a1
pyrus_communis	pycom08g01360 pycom15g01250
rosa_chinensis	RchiOBHm_Chr6g0302551
rosa_laevigata	RLG00000011162
rosa_multiflora	Rmu_sc0007717.1_g000001
rosa_roxburghii	Rroxscaffold_7G00165620
rosa_rugosa	Rorug06G0315000 Rorug06G0315000
rosa_samantha	Rh6AG428300 Rh6BG474400 Rh6CG440400 Rh6DG427400
rosa_wichuraiana	Rw6G037050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	955, 2084
Acc36I	ACCTGC	1 cut(s)	142
Acc65I	GGTACC	1 cut(s)	373
AccB1I	GGYRCC	1 cut(s)	373
AccB7I	CCANNNNNTGG	2 cut(s)	1433, 1460
AccI	GTMKAC	2 cut(s)	2076, 2302
AccII	CGCG	4 cut(s)	475, 591, 1445, 1705
AccIII	TCCGGA	1 cut(s)	1114
AclWI	GGATC	6 cut(s)	1001, 2033, 2190, 2380, 2465, 2534
AcoI	YGGCCR	2 cut(s)	879, 2680
AcsI	RAATTY	6 cut(s)	103, 234, 863, 1464, 2140, 2552
AcuI	CTGAAG	5 cut(s)	843, 1028, 2141, 2171, 2616
AcyI	GRCGYC	1 cut(s)	2253
AfaI	GTAC	4 cut(s)	170, 375, 2068, 2266
AfiI	CCNNNNNNNGG	7 cut(s)	155, 376, 383, 1241, 1433, 1460, 1847
AflII	CTTAAG	1 cut(s)	2062
AflIII	ACRYGT	1 cut(s)	1953
AgsI	TTSAA	9 cut(s)	281, 1029, 1275, 1774, 1902, 1941, 1980, 2363, 2407
AhdI	GACNNNNNGTC	1 cut(s)	1149
AhlI	ACTAGT	1 cut(s)	1697
AjnI	CCWGG	5 cut(s)	566, 1021, 1063, 2178, 2244
AjuI	GAANNNNNNNTTGG	2 cut(s)	1205, 1237
AleI	CACNNNNGTG	1 cut(s)	780
AloI	GAACNNNNNNTCC	2 cut(s)	528, 560
Alw21I	GWGCWC	2 cut(s)	353, 2174
Alw26I	GTCTC	6 cut(s)	167, 543, 709, 941, 2092, 2211
AlwI	GGATC	6 cut(s)	1001, 2033, 2190, 2380, 2465, 2534
AlwNI	CAGNNNCTG	1 cut(s)	1550
Ama87I	CYCGRG	1 cut(s)	2272
Aor13HI	TCCGGA	1 cut(s)	1114
AoxI	GGCC	8 cut(s)	130, 392, 879, 1019, 1395, 1404, 1706, 2680
ApoI	RAATTY	6 cut(s)	103, 234, 863, 1464, 2140, 2552
AseI	ATTAAT	1 cut(s)	1974
Asp700I	GAANNNNTTC	1 cut(s)	2584
Asp718I	GGTACC	1 cut(s)	373
AspLEI	GCGC	3 cut(s)	128, 969, 1687
AspS9I	GGNCC	4 cut(s)	76, 87, 131, 1868
AsuC2I	CCSGG	5 cut(s)	91, 371, 1229, 1473, 1866
AsuHPI	GGTGA	2 cut(s)	1854, 2218
AvaI	CYCGRG	1 cut(s)	2272
AvaII	GGWCC	3 cut(s)	76, 87, 1868
AxyI	CCTNAGG	1 cut(s)	2257
BaeGI	GKGCMC	2 cut(s)	983, 1855
BalI	TGGCCA	1 cut(s)	881
BanI	GGYRCC	1 cut(s)	373
BanII	GRGCYC	2 cut(s)	353, 2174
BbsI	GAAGAC	1 cut(s)	358
Bbv12I	GWGCWC	2 cut(s)	353, 2174
BbvCI	CCTCAGC	1 cut(s)	1317
BccI	CCATC	5 cut(s)	415, 618, 1011, 1427, 1462
BceAI	ACGGC	4 cut(s)	237, 730, 2047, 2667
BcgI	CGANNNNNNTGC	4 cut(s)	305, 339, 2083, 2117
BciT130I	CCWGG	5 cut(s)	568, 1023, 1065, 2180, 2246
BcnI	CCSGG	5 cut(s)	91, 371, 1229, 1473, 1866
BcoDI	GTCTC	6 cut(s)	167, 543, 709, 941, 2092, 2211
BcuI	ACTAGT	1 cut(s)	1697
BfaI	CTAG	5 cut(s)	396, 1295, 1698, 1802, 2235
BfmI	CTRYAG	5 cut(s)	71, 807, 1560, 1617, 2306
BfrI	CTTAAG	1 cut(s)	2062
BfuAI	ACCTGC	1 cut(s)	142
BglI	GCCNNNNNGGC	1 cut(s)	1403
BmcAI	AGTACT	1 cut(s)	2068
Bme18I	GGWCC	3 cut(s)	76, 87, 1868
BmeRI	GACNNNNNGTC	1 cut(s)	1149
BmeT110I	CYCGRG	1 cut(s)	2272
BmgT120I	GGNCC	4 cut(s)	76, 87, 131, 1868
BmiI	GGNNCC	5 cut(s)	88, 89, 375, 1233, 1869
BmrI	ACTGGG	1 cut(s)	1671
BmsI	GCATC	6 cut(s)	26, 1155, 1608, 2332, 2583, 2637
BmuI	ACTGGG	1 cut(s)	1671
BpiI	GAAGAC	1 cut(s)	358
Bpu10I	CCTNAGC	1 cut(s)	1317
BpuEI	CTTGAG	3 cut(s)	2074, 2158, 2188
BpuMI	CCSGG	5 cut(s)	91, 371, 1229, 1473, 1866
BsaBI	GATNNNNATC	1 cut(s)	1005
BsaHI	GRCGYC	1 cut(s)	2253
BsaJI	CCNNGG	7 cut(s)	181, 845, 1064, 1703, 2202, 2212, 2543
BsaWI	WCCGGW	3 cut(s)	376, 1114, 2326
Bsc4I	CCNNNNNNNGG	7 cut(s)	155, 376, 383, 1241, 1433, 1460, 1847
Bse118I	RCCGGY	1 cut(s)	128
Bse1I	ACTGG	6 cut(s)	37, 344, 625, 1514, 1677, 2457
Bse21I	CCTNAGG	1 cut(s)	2257
Bse3DI	GCAATG	2 cut(s)	740, 2284
Bse8I	GATNNNNATC	1 cut(s)	1005
BseAI	TCCGGA	1 cut(s)	1114
BseBI	CCWGG	5 cut(s)	568, 1023, 1065, 2180, 2246
BseDI	CCNNGG	7 cut(s)	181, 845, 1064, 1703, 2202, 2212, 2543
BseJI	GATNNNNATC	1 cut(s)	1005
BseLI	CCNNNNNNNGG	7 cut(s)	155, 376, 383, 1241, 1433, 1460, 1847
BseMI	GCAATG	2 cut(s)	740, 2284
BseNI	ACTGG	6 cut(s)	37, 344, 625, 1514, 1677, 2457
BseRI	GAGGAG	2 cut(s)	32, 968
BseSI	GKGCMC	2 cut(s)	983, 1855
Bsh1236I	CGCG	4 cut(s)	475, 591, 1445, 1705
BshFI	GGCC	8 cut(s)	132, 394, 881, 1021, 1397, 1406, 1708, 2682
BshNI	GGYRCC	1 cut(s)	373
BsiHKAI	GWGCWC	2 cut(s)	353, 2174
BsiHKCI	CYCGRG	1 cut(s)	2272
BsiSI	CCGG	9 cut(s)	91, 129, 371, 377, 1115, 1228, 1472, 1865, 2327
BslFI	GGGAC	4 cut(s)	100, 919, 1617, 2670
BslI	CCNNNNNNNGG	7 cut(s)	155, 376, 383, 1241, 1433, 1460, 1847
BsmAI	GTCTC	6 cut(s)	167, 543, 709, 941, 2092, 2211
BsmBI	CGTCTC	1 cut(s)	709
BsmFI	GGGAC	4 cut(s)	100, 919, 1617, 2670
BsmI	GAATGC	4 cut(s)	1453, 1820, 2131, 2444
BsnI	GGCC	8 cut(s)	132, 394, 881, 1021, 1397, 1406, 1708, 2682
BsoBI	CYCGRG	1 cut(s)	2272
Bsp1286I	GDGCHC	4 cut(s)	353, 983, 1855, 2174
Bsp13I	TCCGGA	1 cut(s)	1114
BspANI	GGCC	8 cut(s)	132, 394, 881, 1021, 1397, 1406, 1708, 2682
BspEI	TCCGGA	1 cut(s)	1114
BspFNI	CGCG	4 cut(s)	475, 591, 1445, 1705
BspLI	GGNNCC	5 cut(s)	88, 89, 375, 1233, 1869
BspMAI	CTGCAG	5 cut(s)	75, 811, 1564, 1621, 2310
BspMI	ACCTGC	1 cut(s)	142
BspPI	GGATC	6 cut(s)	1001, 2033, 2190, 2380, 2465, 2534
BspT107I	GGYRCC	1 cut(s)	373
BspTI	CTTAAG	1 cut(s)	2062
BsrDI	GCAATG	2 cut(s)	740, 2284
BsrFI	RCCGGY	1 cut(s)	128
BsrI	ACTGG	6 cut(s)	37, 344, 625, 1514, 1677, 2457
BssAI	RCCGGY	1 cut(s)	128
BssECI	CCNNGG	7 cut(s)	181, 845, 1064, 1703, 2202, 2212, 2543
BssNI	GRCGYC	1 cut(s)	2253
BssT1I	CCWWGG	4 cut(s)	181, 845, 2212, 2543
Bst2UI	CCWGG	5 cut(s)	568, 1023, 1065, 2180, 2246
Bst4CI	ACNGT	6 cut(s)	1489, 1787, 1906, 2071, 2315, 2639
Bst6I	CTCTTC	1 cut(s)	1834
BstACI	GRCGYC	1 cut(s)	2253
BstAFI	CTTAAG	1 cut(s)	2062
BstAPI	GCANNNNNTGC	1 cut(s)	1625
BstDSI	CCRYGG	1 cut(s)	1703
BstFNI	CGCG	4 cut(s)	475, 591, 1445, 1705
BstHHI	GCGC	3 cut(s)	128, 969, 1687
BstMAI	GTCTC	6 cut(s)	167, 543, 709, 941, 2092, 2211
BstNI	CCWGG	5 cut(s)	568, 1023, 1065, 2180, 2246
BstNSI	RCATGY	6 cut(s)	299, 775, 855, 1822, 1957, 2446
BstSFI	CTRYAG	5 cut(s)	71, 807, 1560, 1617, 2306
BstSLI	GKGCMC	2 cut(s)	983, 1855
BstUI	CGCG	4 cut(s)	475, 591, 1445, 1705
BstV2I	GAAGAC	1 cut(s)	358
BstX2I	RGATCY	4 cut(s)	1006, 2025, 2182, 2372
BstYI	RGATCY	4 cut(s)	1006, 2025, 2182, 2372
Bsu36I	CCTNAGG	1 cut(s)	2257
BsuRI	GGCC	8 cut(s)	132, 394, 881, 1021, 1397, 1406, 1708, 2682
BtgI	CCRYGG	1 cut(s)	1703
BtgZI	GCGATG	1 cut(s)	2660
BtsI	GCAGTG	1 cut(s)	290
BtsIMutI	CAGTG	4 cut(s)	290, 351, 1527, 2644
BveI	ACCTGC	1 cut(s)	142
CaiI	CAGNNNCTG	1 cut(s)	1550
CfoI	GCGC	3 cut(s)	128, 969, 1687
Cfr10I	RCCGGY	1 cut(s)	128
Cfr13I	GGNCC	4 cut(s)	76, 87, 131, 1868
Cfr42I	CCGCGG	1 cut(s)	1706
CseI	GACGC	2 cut(s)	1925, 2261
Csp6I	GTAC	4 cut(s)	169, 374, 2067, 2265
CspCI	CAANNNNNGTGG	2 cut(s)	2002, 2037
CviQI	GTAC	4 cut(s)	169, 374, 2067, 2265
DrdI	GACNNNNNNGTC	2 cut(s)	955, 2084
DriI	GACNNNNNGTC	1 cut(s)	1149
DseDI	GACNNNNNNGTC	2 cut(s)	955, 2084
EaeI	YGGCCR	2 cut(s)	879, 2680
Eam1104I	CTCTTC	1 cut(s)	1834
Eam1105I	GACNNNNNGTC	1 cut(s)	1149
EarI	CTCTTC	1 cut(s)	1834
Ecl136II	GAGCTC	2 cut(s)	351, 2172
Eco130I	CCWWGG	4 cut(s)	181, 845, 2212, 2543
Eco147I	AGGCCT	1 cut(s)	394
Eco24I	GRGCYC	2 cut(s)	353, 2174
Eco47I	GGWCC	3 cut(s)	76, 87, 1868
Eco53kI	GAGCTC	2 cut(s)	351, 2172
Eco57I	CTGAAG	5 cut(s)	843, 1028, 2141, 2171, 2616
Eco81I	CCTNAGG	1 cut(s)	2257
Eco88I	CYCGRG	1 cut(s)	2272
EcoICRI	GAGCTC	2 cut(s)	351, 2172
EcoO109I	RGGNCCY	2 cut(s)	87, 1868
EcoRI	GAATTC	2 cut(s)	1464, 2140
EcoRII	CCWGG	5 cut(s)	566, 1021, 1063, 2178, 2244
EcoT14I	CCWWGG	4 cut(s)	181, 845, 2212, 2543
EcoT22I	ATGCAT	2 cut(s)	1820, 2444
EcoT38I	GRGCYC	2 cut(s)	353, 2174
ErhI	CCWWGG	4 cut(s)	181, 845, 2212, 2543
Esp3I	CGTCTC	1 cut(s)	709
FaqI	GGGAC	4 cut(s)	100, 919, 1617, 2670
FauI	CCCGC	2 cut(s)	141, 584
FauNDI	CATATG	1 cut(s)	1644
FblI	GTMKAC	2 cut(s)	2076, 2302
FriOI	GRGCYC	2 cut(s)	353, 2174
FspBI	CTAG	5 cut(s)	396, 1295, 1698, 1802, 2235
GlaI	GCGC	3 cut(s)	127, 968, 1686
HaeIII	GGCC	8 cut(s)	132, 394, 881, 1021, 1397, 1406, 1708, 2682
HapII	CCGG	9 cut(s)	91, 129, 371, 377, 1115, 1228, 1472, 1865, 2327
HgaI	GACGC	2 cut(s)	1925, 2261
HhaI	GCGC	3 cut(s)	128, 969, 1687
Hin1I	GRCGYC	1 cut(s)	2253
Hin6I	GCGC	3 cut(s)	126, 967, 1685
HinP1I	GCGC	3 cut(s)	126, 967, 1685
HincII	GTYRAC	1 cut(s)	2077
HindII	GTYRAC	1 cut(s)	2077
HindIII	AAGCTT	1 cut(s)	2627
HpaII	CCGG	9 cut(s)	91, 129, 371, 377, 1115, 1228, 1472, 1865, 2327
HphI	GGTGA	2 cut(s)	1854, 2218
Hpy166II	GTNNAC	6 cut(s)	401, 1441, 1790, 1889, 2077, 2303
Hpy188III	TCNNGA	7 cut(s)	916, 946, 1115, 1173, 1235, 1949, 2137
Hpy8I	GTNNAC	6 cut(s)	401, 1441, 1790, 1889, 2077, 2303
Hpy99I	CGWCG	4 cut(s)	214, 317, 2078, 2522
HpyCH4III	ACNGT	6 cut(s)	1489, 1787, 1906, 2071, 2315, 2639
HpyCH4IV	ACGT	1 cut(s)	2520
HpySE526I	ACGT	1 cut(s)	2520
Hsp92I	GRCGYC	1 cut(s)	2253
HspAI	GCGC	3 cut(s)	126, 967, 1685
KflI	GGGWCCC	1 cut(s)	87
Kpn2I	TCCGGA	1 cut(s)	1114
KpnI	GGTACC	1 cut(s)	377
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
KspI	CCGCGG	1 cut(s)	1706
LmnI	GCTCC	2 cut(s)	109, 1184
LweI	GCATC	6 cut(s)	26, 1155, 1608, 2332, 2583, 2637
MaeI	CTAG	5 cut(s)	396, 1295, 1698, 1802, 2235
MaeII	ACGT	1 cut(s)	2520
MaeIII	GTNAC	3 cut(s)	286, 440, 2395
MfeI	CAATTG	1 cut(s)	2388
MflI	RGATCY	4 cut(s)	1006, 2025, 2182, 2372
MhlI	GDGCHC	4 cut(s)	353, 983, 1855, 2174
MlsI	TGGCCA	1 cut(s)	881
MluNI	TGGCCA	1 cut(s)	881
MlyI	GAGTC	6 cut(s)	169, 829, 965, 1159, 1639, 2213
MmeI	TCCRAC	2 cut(s)	1498, 1980
Mox20I	TGGCCA	1 cut(s)	881
Mph1103I	ATGCAT	2 cut(s)	1820, 2444
MroI	TCCGGA	1 cut(s)	1114
MroNI	GCCGGC	1 cut(s)	128
MroXI	GAANNNNTTC	1 cut(s)	2584
MscI	TGGCCA	1 cut(s)	881
MseI	TTAA	8 cut(s)	339, 1155, 1326, 1571, 1910, 1974, 2063, 2160
MslI	CAYNNNNRTG	1 cut(s)	780
Msp20I	TGGCCA	1 cut(s)	881
MspA1I	CMGCKG	7 cut(s)	485, 505, 1012, 1430, 1654, 1705, 2571
MspCI	CTTAAG	1 cut(s)	2062
MspI	CCGG	9 cut(s)	91, 129, 371, 377, 1115, 1228, 1472, 1865, 2327
MunI	CAATTG	1 cut(s)	2388
Mva1269I	GAATGC	4 cut(s)	1453, 1820, 2131, 2444
MvaI	CCWGG	5 cut(s)	568, 1023, 1065, 2180, 2246
MvnI	CGCG	4 cut(s)	475, 591, 1445, 1705
NaeI	GCCGGC	1 cut(s)	130
NciI	CCSGG	5 cut(s)	91, 371, 1229, 1473, 1866
NdeI	CATATG	1 cut(s)	1644
NgoMIV	GCCGGC	1 cut(s)	128
NlaIV	GGNNCC	5 cut(s)	88, 89, 375, 1233, 1869
NmuCI	GTSAC	1 cut(s)	440
NsiI	ATGCAT	2 cut(s)	1820, 2444
NspI	RCATGY	6 cut(s)	299, 775, 855, 1822, 1957, 2446
OliI	CACNNNNGTG	1 cut(s)	780
PaeI	GCATGC	3 cut(s)	299, 775, 855
PaeR7I	CTCGAG	1 cut(s)	2272
PceI	AGGCCT	1 cut(s)	394
PciI	ACATGT	1 cut(s)	1953
PcsI	WCGNNNNNNNCGW	1 cut(s)	724
PctI	GAATGC	4 cut(s)	1453, 1820, 2131, 2444
PdiI	GCCGGC	1 cut(s)	130
PdmI	GAANNNNTTC	1 cut(s)	2584
PfeI	GAWTC	9 cut(s)	665, 1176, 1361, 1379, 1423, 1648, 1724, 1895, 2580
PflMI	CCANNNNNTGG	2 cut(s)	1433, 1460
PleI	GAGTC	6 cut(s)	168, 828, 964, 1158, 1638, 2213
PpsI	GAGTC	6 cut(s)	168, 828, 964, 1158, 1638, 2213
PpuMI	RGGWCCY	2 cut(s)	87, 1868
PscI	ACATGT	1 cut(s)	1953
PshBI	ATTAAT	1 cut(s)	1974
Psp124BI	GAGCTC	2 cut(s)	353, 2174
Psp5II	RGGWCCY	2 cut(s)	87, 1868
Psp6I	CCWGG	5 cut(s)	566, 1021, 1063, 2178, 2244
PspGI	CCWGG	5 cut(s)	566, 1021, 1063, 2178, 2244
PspN4I	GGNNCC	5 cut(s)	88, 89, 375, 1233, 1869
PspPI	GGNCC	4 cut(s)	76, 87, 131, 1868
PspPPI	RGGWCCY	2 cut(s)	87, 1868
PspXI	VCTCGAGB	1 cut(s)	2272
PsrI	GAACNNNNNNTAC	2 cut(s)	1330, 1362
PstI	CTGCAG	5 cut(s)	75, 811, 1564, 1621, 2310
PstNI	CAGNNNCTG	1 cut(s)	1550
PsuI	RGATCY	4 cut(s)	1006, 2025, 2182, 2372
PvuII	CAGCTG	2 cut(s)	1430, 1654
RsaI	GTAC	4 cut(s)	170, 375, 2068, 2266
RsaNI	GTAC	4 cut(s)	169, 374, 2067, 2265
RseI	CAYNNNNRTG	1 cut(s)	780
SacI	GAGCTC	2 cut(s)	353, 2174
SacII	CCGCGG	1 cut(s)	1706
SalI	GTCGAC	1 cut(s)	2075
SaqAI	TTAA	8 cut(s)	339, 1155, 1326, 1571, 1910, 1974, 2063, 2160
Sau96I	GGNCC	4 cut(s)	76, 87, 131, 1868
ScaI	AGTACT	1 cut(s)	2068
SchI	GAGTC	6 cut(s)	169, 829, 965, 1159, 1639, 2213
SduI	GDGCHC	4 cut(s)	353, 983, 1855, 2174
SfaNI	GCATC	6 cut(s)	26, 1155, 1608, 2332, 2583, 2637
SfcI	CTRYAG	5 cut(s)	71, 807, 1560, 1617, 2306
SfiI	GGCCNNNNNGGCC	1 cut(s)	1403
Sfr274I	CTCGAG	1 cut(s)	2272
Sfr303I	CCGCGG	1 cut(s)	1706
SgrBI	CCGCGG	1 cut(s)	1706
SinI	GGWCC	3 cut(s)	76, 87, 1868
SlaI	CTCGAG	1 cut(s)	2272
SmiMI	CAYNNNNRTG	1 cut(s)	780
SmlI	CTYRAG	5 cut(s)	2062, 2089, 2167, 2173, 2272
SmoI	CTYRAG	5 cut(s)	2062, 2089, 2167, 2173, 2272
SpeI	ACTAGT	1 cut(s)	1697
SphI	GCATGC	3 cut(s)	299, 775, 855
SseBI	AGGCCT	1 cut(s)	394
SspMI	CTAG	5 cut(s)	396, 1295, 1698, 1802, 2235
SstI	GAGCTC	2 cut(s)	353, 2174
StuI	AGGCCT	1 cut(s)	394
StyI	CCWWGG	4 cut(s)	181, 845, 2212, 2543
TaaI	ACNGT	6 cut(s)	1489, 1787, 1906, 2071, 2315, 2639
TaiI	ACGT	1 cut(s)	2523
TaqII	GACCGA	1 cut(s)	1934
TatI	WGTACW	1 cut(s)	2066
TauI	GCSGC	4 cut(s)	151, 475, 800, 1708
TfiI	GAWTC	9 cut(s)	665, 1176, 1361, 1379, 1423, 1648, 1724, 1895, 2580
Tru1I	TTAA	8 cut(s)	339, 1155, 1326, 1571, 1910, 1974, 2063, 2160
Tru9I	TTAA	8 cut(s)	339, 1155, 1326, 1571, 1910, 1974, 2063, 2160
TscAI	CASTG	4 cut(s)	297, 351, 1534, 2644
TseFI	GTSAC	1 cut(s)	440
Tsp45I	GTSAC	1 cut(s)	440
TspGWI	ACGGA	2 cut(s)	457, 658
TspRI	CASTG	4 cut(s)	297, 351, 1534, 2644
Van91I	CCANNNNNTGG	2 cut(s)	1433, 1460
Vha464I	CTTAAG	1 cut(s)	2062
VpaK11BI	GGWCC	3 cut(s)	76, 87, 1868
VspI	ATTAAT	1 cut(s)	1974
XapI	RAATTY	6 cut(s)	103, 234, 863, 1464, 2140, 2552
XceI	RCATGY	6 cut(s)	299, 775, 855, 1822, 1957, 2446
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1514
XhoI	CTCGAG	1 cut(s)	2272
XmiI	GTMKAC	2 cut(s)	2076, 2302
XmnI	GAANNNNTTC	1 cut(s)	2584
XspI	CTAG	5 cut(s)	396, 1295, 1698, 1802, 2235
ZrmI	AGTACT	1 cut(s)	2068
Zsp2I	ATGCAT	2 cut(s)	1820, 2444

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.