Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G19850 AT1G19850 |
| fragaria_vesca | FvH4_2g38760 FvH4_2g38760 FvH4_2g38760 |
| malus_domestica | MD08G1015500.v1.1 MD15G1014400.v1.1 |
| prunus_persica | Prupe.1G368300_v2.0.a1 |
| pyrus_communis | pycom08g01360 pycom15g01250 |
| rosa_chinensis | RchiOBHm_Chr6g0302551 |
| rosa_laevigata | RLG00000011162 |
| rosa_multiflora | Rmu_sc0007717.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00165620 |
| rosa_rugosa | Rorug06G0315000 Rorug06G0315000 |
| rosa_samantha | Rh6AG428300 Rh6BG474400 Rh6CG440400 Rh6DG427400 |
| rosa_wichuraiana | Rw6G037050 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 202, 1408 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 928, 970 |
| Acc36I | ACCTGC | 2 cut(s) | 202, 1408 |
| AccB1I | GGYRCC | 1 cut(s) | 2382 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 398 |
| AccI | GTMKAC | 3 cut(s) | 55, 2193, 2365 |
| AccII | CGCG | 2 cut(s) | 490, 606 |
| AciI | CCGC | 7 cut(s) | 488, 490, 539, 604, 606, 1976, 2429 |
| AclWI | GGATC | 5 cut(s) | 99, 1016, 2253, 2440, 2594 |
| AcoI | YGGCCR | 1 cut(s) | 607 |
| AcsI | RAATTY | 6 cut(s) | 118, 249, 441, 878, 899, 2612 |
| AcuI | CTGAAG | 5 cut(s) | 425, 858, 1043, 1584, 1926 |
| AdeI | CACNNNGTG | 2 cut(s) | 473, 2389 |
| AfaI | GTAC | 4 cut(s) | 390, 997, 2116, 2329 |
| AfeI | AGCGCT | 1 cut(s) | 2701 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 129, 170, 398, 1256, 1678, 1838, 1839, 2039, 2083 |
| AgsI | TTSAA | 5 cut(s) | 296, 1495, 1572, 1932, 2423 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1164 |
| AhlI | ACTAGT | 2 cut(s) | 2459, 2554 |
| AjnI | CCWGG | 5 cut(s) | 581, 1036, 1341, 1677, 1855 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2205, 2237 |
| AleI | CACNNNNGTG | 3 cut(s) | 346, 795, 2729 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 543, 575 |
| Alw21I | GWGCWC | 3 cut(s) | 46, 1598, 2237 |
| Alw26I | GTCTC | 5 cut(s) | 279, 558, 956, 1118, 1470 |
| AlwI | GGATC | 5 cut(s) | 99, 1016, 2253, 2440, 2594 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1208, 2248 |
| Ama87I | CYCGRG | 1 cut(s) | 2236 |
| Aor51HI | AGCGCT | 1 cut(s) | 2701 |
| AoxI | GGCC | 9 cut(s) | 111, 145, 199, 607, 1034, 1215, 1410, 1681, 1859 |
| ApoI | RAATTY | 6 cut(s) | 118, 249, 441, 878, 899, 2612 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 257, 289 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2644 |
| AspA2I | CCTAGG | 1 cut(s) | 2275 |
| AspLEI | GCGC | 4 cut(s) | 143, 621, 2631, 2702 |
| AspS9I | GGNCC | 9 cut(s) | 102, 146, 199, 921, 1215, 1391, 1681, 1859, 2392 |
| AsuC2I | CCSGG | 3 cut(s) | 106, 1080, 1244 |
| AsuHPI | GGTGA | 2 cut(s) | 1432, 1845 |
| AsuNHI | GCTAGC | 1 cut(s) | 1694 |
| AvaI | CYCGRG | 1 cut(s) | 2236 |
| AvaII | GGWCC | 4 cut(s) | 102, 921, 1391, 2392 |
| AvrII | CCTAGG | 1 cut(s) | 2275 |
| AxyI | CCTNAGG | 2 cut(s) | 195, 1332 |
| BaeI | ACNNNNGTAYC | 8 cut(s) | 979, 987, 1012, 1020, 1183, 1216, 2106, 2139 |
| BanI | GGYRCC | 1 cut(s) | 2382 |
| BanII | GRGCYC | 2 cut(s) | 1598, 2237 |
| Bbv12I | GWGCWC | 3 cut(s) | 46, 1598, 2237 |
| BccI | CCATC | 5 cut(s) | 769, 1026, 1900, 2010, 2020 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 775, 809 |
| BciT130I | CCWGG | 5 cut(s) | 583, 1038, 1343, 1679, 1857 |
| BciVI | GTATCC | 3 cut(s) | 232, 1184, 1836 |
| BclI | TGATCA | 2 cut(s) | 1576, 1984 |
| BcnI | CCSGG | 3 cut(s) | 106, 1080, 1244 |
| BcoDI | GTCTC | 5 cut(s) | 279, 558, 956, 1118, 1470 |
| BcuI | ACTAGT | 2 cut(s) | 2459, 2554 |
| BfaI | CTAG | 9 cut(s) | 51, 411, 1310, 1347, 1397, 1695, 2276, 2460, 2555 |
| BfmI | CTRYAG | 6 cut(s) | 86, 810, 1469, 2249, 2296, 2369 |
| BfoI | RGCGCY | 2 cut(s) | 622, 2703 |
| BfuAI | ACCTGC | 2 cut(s) | 202, 1408 |
| BfuI | GTATCC | 3 cut(s) | 232, 1184, 1836 |
| BglII | AGATCT | 1 cut(s) | 333 |
| BlnI | CCTAGG | 1 cut(s) | 2275 |
| Bme1390I | CCNGG | 8 cut(s) | 106, 583, 1038, 1080, 1244, 1343, 1679, 1857 |
| Bme18I | GGWCC | 4 cut(s) | 102, 921, 1391, 2392 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1164 |
| BmeT110I | CYCGRG | 1 cut(s) | 2236 |
| BmgT120I | GGNCC | 9 cut(s) | 102, 146, 199, 921, 1215, 1391, 1681, 1859, 2392 |
| BmiI | GGNNCC | 9 cut(s) | 103, 104, 148, 922, 923, 1042, 1248, 2384, 2394 |
| BmrFI | CCNGG | 8 cut(s) | 106, 583, 1038, 1080, 1244, 1343, 1679, 1857 |
| BmrI | ACTGGG | 1 cut(s) | 1483 |
| BmsI | GCATC | 2 cut(s) | 427, 2643 |
| BmtI | GCTAGC | 1 cut(s) | 1698 |
| BmuI | ACTGGG | 1 cut(s) | 1483 |
| BpuEI | CTTGAG | 4 cut(s) | 80, 150, 2251, 2320 |
| BpuMI | CCSGG | 3 cut(s) | 106, 1080, 1244 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1020 |
| BsaWI | WCCGGW | 1 cut(s) | 2321 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 56, 86 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 129, 170, 398, 1256, 1678, 1838, 1839, 2039, 2083 |
| Bse118I | RCCGGY | 1 cut(s) | 143 |
| Bse1I | ACTGG | 8 cut(s) | 359, 640, 1489, 1526, 1588, 2122, 2313, 2517 |
| Bse21I | CCTNAGG | 2 cut(s) | 195, 1332 |
| Bse3DI | GCAATG | 2 cut(s) | 1618, 2347 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1020 |
| BseBI | CCWGG | 5 cut(s) | 583, 1038, 1343, 1679, 1857 |
| BseGI | GGATG | 8 cut(s) | 418, 706, 761, 1997, 2173, 2330, 2602, 2724 |
| BseJI | GATNNNNATC | 1 cut(s) | 1020 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 129, 170, 398, 1256, 1678, 1838, 1839, 2039, 2083 |
| BseMI | GCAATG | 2 cut(s) | 1618, 2347 |
| BseNI | ACTGG | 8 cut(s) | 359, 640, 1489, 1526, 1588, 2122, 2313, 2517 |
| BseRI | GAGGAG | 3 cut(s) | 39, 77, 983 |
| BseYI | CCCAGC | 2 cut(s) | 1631, 1838 |
| BsgI | GTGCAG | 1 cut(s) | 1221 |
| Bsh1236I | CGCG | 2 cut(s) | 490, 606 |
| BshFI | GGCC | 9 cut(s) | 113, 147, 201, 609, 1036, 1217, 1412, 1683, 1861 |
| BshNI | GGYRCC | 1 cut(s) | 2382 |
| BsiHKAI | GWGCWC | 3 cut(s) | 46, 1598, 2237 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2236 |
| BsiSI | CCGG | 6 cut(s) | 106, 144, 975, 1079, 1243, 2322 |
| BslFI | GGGAC | 5 cut(s) | 88, 934, 1085, 2239, 2378 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 129, 170, 398, 1256, 1678, 1838, 1839, 2039, 2083 |
| BsmAI | GTCTC | 5 cut(s) | 279, 558, 956, 1118, 1470 |
| BsmBI | CGTCTC | 1 cut(s) | 279 |
| BsmFI | GGGAC | 5 cut(s) | 88, 934, 1085, 2239, 2378 |
| BsmI | GAATGC | 2 cut(s) | 1501, 1820 |
| BsnI | GGCC | 9 cut(s) | 113, 147, 201, 609, 1036, 1217, 1412, 1683, 1861 |
| BsoBI | CYCGRG | 1 cut(s) | 2236 |
| Bsp1286I | GDGCHC | 3 cut(s) | 46, 1598, 2237 |
| BspACI | CCGC | 7 cut(s) | 488, 490, 539, 604, 606, 1976, 2429 |
| BspANI | GGCC | 9 cut(s) | 113, 147, 201, 609, 1036, 1217, 1412, 1683, 1861 |
| BspFNI | CGCG | 2 cut(s) | 490, 606 |
| BspLI | GGNNCC | 9 cut(s) | 103, 104, 148, 922, 923, 1042, 1248, 2384, 2394 |
| BspMAI | CTGCAG | 6 cut(s) | 90, 814, 1473, 2253, 2300, 2373 |
| BspMI | ACCTGC | 2 cut(s) | 202, 1408 |
| BspOI | GCTAGC | 1 cut(s) | 1698 |
| BspPI | GGATC | 5 cut(s) | 99, 1016, 2253, 2440, 2594 |
| BspQI | GCTCTTC | 1 cut(s) | 1603 |
| BspT107I | GGYRCC | 1 cut(s) | 2382 |
| BsrDI | GCAATG | 2 cut(s) | 1618, 2347 |
| BsrFI | RCCGGY | 1 cut(s) | 143 |
| BsrI | ACTGG | 8 cut(s) | 359, 640, 1489, 1526, 1588, 2122, 2313, 2517 |
| BssAI | RCCGGY | 1 cut(s) | 143 |
| BssNAI | GTATAC | 1 cut(s) | 56 |
| BssT1I | CCWWGG | 5 cut(s) | 519, 860, 2275, 2415, 2603 |
| Bst1107I | GTATAC | 1 cut(s) | 56 |
| Bst2UI | CCWGG | 5 cut(s) | 583, 1038, 1343, 1679, 1857 |
| Bst4CI | ACNGT | 3 cut(s) | 48, 930, 1451 |
| Bst6I | CTCTTC | 2 cut(s) | 1603, 2670 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 818, 1414 |
| BstDSI | CCRYGG | 3 cut(s) | 488, 604, 1700 |
| BstF5I | GGATG | 8 cut(s) | 418, 706, 761, 1997, 2173, 2330, 2602, 2724 |
| BstFNI | CGCG | 2 cut(s) | 490, 606 |
| BstH2I | RGCGCY | 2 cut(s) | 622, 2703 |
| BstHHI | GCGC | 4 cut(s) | 143, 621, 2631, 2702 |
| BstMAI | GTCTC | 5 cut(s) | 279, 558, 956, 1118, 1470 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 415, 812, 818, 821, 1414, 1593, 1637, 1975, 2232 |
| BstNI | CCWGG | 5 cut(s) | 583, 1038, 1343, 1679, 1857 |
| BstNSI | RCATGY | 7 cut(s) | 137, 790, 870, 1665, 1822, 2192, 2506 |
| BstSCI | CCNGG | 8 cut(s) | 104, 581, 1036, 1078, 1242, 1341, 1677, 1855 |
| BstSFI | CTRYAG | 6 cut(s) | 86, 810, 1469, 2249, 2296, 2369 |
| BstUI | CGCG | 2 cut(s) | 490, 606 |
| BstX2I | RGATCY | 3 cut(s) | 333, 2245, 2432 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1032 |
| BstYI | RGATCY | 3 cut(s) | 333, 2245, 2432 |
| BstZ17I | GTATAC | 1 cut(s) | 56 |
| Bsu36I | CCTNAGG | 2 cut(s) | 195, 1332 |
| BsuI | GTATCC | 3 cut(s) | 232, 1184, 1836 |
| BsuRI | GGCC | 9 cut(s) | 113, 147, 201, 609, 1036, 1217, 1412, 1683, 1861 |
| BtgI | CCRYGG | 3 cut(s) | 488, 604, 1700 |
| BtsCI | GGATG | 8 cut(s) | 418, 706, 761, 1997, 2173, 2330, 2602, 2724 |
| BtsIMutI | CAGTG | 2 cut(s) | 1025, 1539 |
| BveI | ACCTGC | 2 cut(s) | 202, 1408 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1208, 2248 |
| CfoI | GCGC | 4 cut(s) | 143, 621, 2631, 2702 |
| Cfr10I | RCCGGY | 1 cut(s) | 143 |
| Cfr13I | GGNCC | 9 cut(s) | 102, 146, 199, 921, 1215, 1391, 1681, 1859, 2392 |
| Cfr42I | CCGCGG | 2 cut(s) | 491, 607 |
| CseI | GACGC | 1 cut(s) | 1654 |
| Csp6I | GTAC | 4 cut(s) | 389, 996, 2115, 2328 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 2062, 2097, 2305, 2340 |
| CviQI | GTAC | 4 cut(s) | 389, 996, 2115, 2328 |
| DraIII | CACNNNGTG | 2 cut(s) | 473, 2389 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 928, 970 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1164 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 928, 970 |
| EaeI | YGGCCR | 1 cut(s) | 607 |
| Eam1104I | CTCTTC | 2 cut(s) | 1603, 2670 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1164 |
| EarI | CTCTTC | 2 cut(s) | 1603, 2670 |
| Ecl136II | GAGCTC | 2 cut(s) | 1596, 2235 |
| Eco130I | CCWWGG | 5 cut(s) | 519, 860, 2275, 2415, 2603 |
| Eco24I | GRGCYC | 2 cut(s) | 1598, 2237 |
| Eco47I | GGWCC | 4 cut(s) | 102, 921, 1391, 2392 |
| Eco47III | AGCGCT | 1 cut(s) | 2701 |
| Eco53kI | GAGCTC | 2 cut(s) | 1596, 2235 |
| Eco57I | CTGAAG | 5 cut(s) | 425, 858, 1043, 1584, 1926 |
| Eco81I | CCTNAGG | 2 cut(s) | 195, 1332 |
| Eco88I | CYCGRG | 1 cut(s) | 2236 |
| EcoICRI | GAGCTC | 2 cut(s) | 1596, 2235 |
| EcoO109I | RGGNCCY | 3 cut(s) | 102, 921, 1859 |
| EcoRII | CCWGG | 5 cut(s) | 581, 1036, 1341, 1677, 1855 |
| EcoT14I | CCWWGG | 5 cut(s) | 519, 860, 2275, 2415, 2603 |
| EcoT22I | ATGCAT | 2 cut(s) | 1663, 1820 |
| EcoT38I | GRGCYC | 2 cut(s) | 1598, 2237 |
| ErhI | CCWWGG | 5 cut(s) | 519, 860, 2275, 2415, 2603 |
| Esp3I | CGTCTC | 1 cut(s) | 279 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2508, 2540 |
| FaqI | GGGAC | 5 cut(s) | 88, 934, 1085, 2239, 2378 |
| FauI | CCCGC | 1 cut(s) | 2422 |
| FauNDI | CATATG | 1 cut(s) | 1873 |
| FbaI | TGATCA | 2 cut(s) | 1576, 1984 |
| FblI | GTMKAC | 3 cut(s) | 55, 2193, 2365 |
| FokI | GGATG | 8 cut(s) | 405, 713, 748, 1984, 2180, 2317, 2609, 2731 |
| FriOI | GRGCYC | 2 cut(s) | 1598, 2237 |
| FspBI | CTAG | 9 cut(s) | 51, 411, 1310, 1347, 1397, 1695, 2276, 2460, 2555 |
| GlaI | GCGC | 4 cut(s) | 142, 620, 2630, 2701 |
| GsaI | CCCAGC | 2 cut(s) | 1635, 1842 |
| HaeII | RGCGCY | 2 cut(s) | 622, 2703 |
| HaeIII | GGCC | 9 cut(s) | 113, 147, 201, 609, 1036, 1217, 1412, 1683, 1861 |
| HapII | CCGG | 6 cut(s) | 106, 144, 975, 1079, 1243, 2322 |
| HgaI | GACGC | 1 cut(s) | 1654 |
| HhaI | GCGC | 4 cut(s) | 143, 621, 2631, 2702 |
| Hin6I | GCGC | 4 cut(s) | 141, 619, 2629, 2700 |
| HinP1I | GCGC | 4 cut(s) | 141, 619, 2629, 2700 |
| HincII | GTYRAC | 3 cut(s) | 1537, 1669, 2194 |
| HindII | GTYRAC | 3 cut(s) | 1537, 1669, 2194 |
| HindIII | AAGCTT | 1 cut(s) | 2687 |
| HpaI | GTTAAC | 1 cut(s) | 1537 |
| HpaII | CCGG | 6 cut(s) | 106, 144, 975, 1079, 1243, 2322 |
| HphI | GGTGA | 2 cut(s) | 1432, 1845 |
| Hpy166II | GTNNAC | 9 cut(s) | 56, 1394, 1447, 1537, 1669, 1790, 2194, 2366, 2566 |
| Hpy188III | TCNNGA | 9 cut(s) | 1130, 1188, 1250, 1602, 1940, 2006, 2200, 2243, 2351 |
| Hpy8I | GTNNAC | 9 cut(s) | 56, 1394, 1447, 1537, 1669, 1790, 2194, 2366, 2566 |
| HpyAV | CCTTC | 8 cut(s) | 103, 635, 882, 1161, 1494, 2154, 2217, 2510 |
| HpyCH4III | ACNGT | 3 cut(s) | 48, 930, 1451 |
| HpyCH4IV | ACGT | 2 cut(s) | 1174, 1459 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 415, 812, 818, 821, 1414, 1593, 1637, 1975, 2232 |
| HpySE526I | ACGT | 2 cut(s) | 1174, 1459 |
| HspAI | GCGC | 4 cut(s) | 141, 619, 2629, 2700 |
| KflI | GGGWCCC | 2 cut(s) | 102, 921 |
| KroI | GCCGGC | 1 cut(s) | 143 |
| KroNI | GCCGGC | 1 cut(s) | 145 |
| Ksp22I | TGATCA | 2 cut(s) | 1576, 1984 |
| KspAI | GTTAAC | 1 cut(s) | 1537 |
| KspI | CCGCGG | 2 cut(s) | 491, 607 |
| LguI | GCTCTTC | 1 cut(s) | 1603 |
| LmnI | GCTCC | 1 cut(s) | 41 |
| LweI | GCATC | 2 cut(s) | 427, 2643 |
| MaeI | CTAG | 9 cut(s) | 51, 411, 1310, 1347, 1397, 1695, 2276, 2460, 2555 |
| MaeII | ACGT | 2 cut(s) | 1174, 1459 |
| MaeIII | GTNAC | 5 cut(s) | 301, 708, 2149, 2455, 2462 |
| MboII | GAAGA | 7 cut(s) | 17, 22, 328, 1049, 1412, 1590, 2687 |
| MfeI | CAATTG | 2 cut(s) | 2448, 2493 |
| MflI | RGATCY | 3 cut(s) | 333, 2245, 2432 |
| MhlI | GDGCHC | 3 cut(s) | 46, 1598, 2237 |
| MlyI | GAGTC | 7 cut(s) | 184, 980, 1120, 1174, 1483, 1880, 2248 |
| MmeI | TCCRAC | 1 cut(s) | 620 |
| Mph1103I | ATGCAT | 2 cut(s) | 1663, 1820 |
| MroNI | GCCGGC | 1 cut(s) | 143 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2644 |
| MslI | CAYNNNNRTG | 9 cut(s) | 344, 346, 423, 795, 1030, 1418, 1795, 2162, 2729 |
| MspA1I | CMGCKG | 2 cut(s) | 490, 606 |
| MspI | CCGG | 6 cut(s) | 106, 144, 975, 1079, 1243, 2322 |
| MspR9I | CCNGG | 8 cut(s) | 106, 583, 1038, 1080, 1244, 1343, 1679, 1857 |
| MunI | CAATTG | 2 cut(s) | 2448, 2493 |
| Mva1269I | GAATGC | 2 cut(s) | 1501, 1820 |
| MvaI | CCWGG | 5 cut(s) | 583, 1038, 1343, 1679, 1857 |
| MvnI | CGCG | 2 cut(s) | 490, 606 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 415, 812, 818, 821, 1414, 1593, 1637, 1975, 2232 |
| NaeI | GCCGGC | 1 cut(s) | 145 |
| NciI | CCSGG | 3 cut(s) | 106, 1080, 1244 |
| NdeI | CATATG | 1 cut(s) | 1873 |
| NgoMIV | GCCGGC | 1 cut(s) | 143 |
| NheI | GCTAGC | 1 cut(s) | 1694 |
| NlaIV | GGNNCC | 9 cut(s) | 103, 104, 148, 922, 923, 1042, 1248, 2384, 2394 |
| NmuCI | GTSAC | 1 cut(s) | 708 |
| NsiI | ATGCAT | 2 cut(s) | 1663, 1820 |
| NspI | RCATGY | 7 cut(s) | 137, 790, 870, 1665, 1822, 2192, 2506 |
| OliI | CACNNNNGTG | 3 cut(s) | 346, 795, 2729 |
| PaeI | GCATGC | 4 cut(s) | 137, 790, 870, 1665 |
| PaeR7I | CTCGAG | 1 cut(s) | 2236 |
| PaqCI | CACCTGC | 2 cut(s) | 202, 1408 |
| PciSI | GCTCTTC | 1 cut(s) | 1603 |
| PctI | GAATGC | 2 cut(s) | 1501, 1820 |
| PdiI | GCCGGC | 1 cut(s) | 145 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2644 |
| PfeI | GAWTC | 8 cut(s) | 265, 680, 1373, 1438, 1724, 2203, 2282, 2640 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 398 |
| PfoI | TCCNGGA | 1 cut(s) | 104 |
| PleI | GAGTC | 7 cut(s) | 183, 979, 1120, 1173, 1482, 1880, 2247 |
| PpsI | GAGTC | 7 cut(s) | 183, 979, 1120, 1173, 1482, 1880, 2247 |
| PpuMI | RGGWCCY | 2 cut(s) | 102, 921 |
| Psp124BI | GAGCTC | 2 cut(s) | 1598, 2237 |
| Psp5II | RGGWCCY | 2 cut(s) | 102, 921 |
| Psp6I | CCWGG | 5 cut(s) | 581, 1036, 1341, 1677, 1855 |
| PspFI | CCCAGC | 2 cut(s) | 1631, 1838 |
| PspGI | CCWGG | 5 cut(s) | 581, 1036, 1341, 1677, 1855 |
| PspN4I | GGNNCC | 9 cut(s) | 103, 104, 148, 922, 923, 1042, 1248, 2384, 2394 |
| PspPI | GGNCC | 9 cut(s) | 102, 146, 199, 921, 1215, 1391, 1681, 1859, 2392 |
| PspPPI | RGGWCCY | 2 cut(s) | 102, 921 |
| PspXI | VCTCGAGB | 1 cut(s) | 2236 |
| PstI | CTGCAG | 6 cut(s) | 90, 814, 1473, 2253, 2300, 2373 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1208, 2248 |
| PsuI | RGATCY | 3 cut(s) | 333, 2245, 2432 |
| RsaI | GTAC | 4 cut(s) | 390, 997, 2116, 2329 |
| RsaNI | GTAC | 4 cut(s) | 389, 996, 2115, 2328 |
| RseI | CAYNNNNRTG | 9 cut(s) | 344, 346, 423, 795, 1030, 1418, 1795, 2162, 2729 |
| SacI | GAGCTC | 2 cut(s) | 1598, 2237 |
| SacII | CCGCGG | 2 cut(s) | 491, 607 |
| SalI | GTCGAC | 1 cut(s) | 2192 |
| SapI | GCTCTTC | 1 cut(s) | 1603 |
| Sau96I | GGNCC | 9 cut(s) | 102, 146, 199, 921, 1215, 1391, 1681, 1859, 2392 |
| SchI | GAGTC | 7 cut(s) | 184, 980, 1120, 1174, 1483, 1880, 2248 |
| ScrFI | CCNGG | 8 cut(s) | 106, 583, 1038, 1080, 1244, 1343, 1679, 1857 |
| SduI | GDGCHC | 3 cut(s) | 46, 1598, 2237 |
| SfaNI | GCATC | 2 cut(s) | 427, 2643 |
| SfcI | CTRYAG | 6 cut(s) | 86, 810, 1469, 2249, 2296, 2369 |
| Sfr274I | CTCGAG | 1 cut(s) | 2236 |
| Sfr303I | CCGCGG | 2 cut(s) | 491, 607 |
| SgrBI | CCGCGG | 2 cut(s) | 491, 607 |
| SinI | GGWCC | 4 cut(s) | 102, 921, 1391, 2392 |
| SlaI | CTCGAG | 1 cut(s) | 2236 |
| SmiMI | CAYNNNNRTG | 9 cut(s) | 344, 346, 423, 795, 1030, 1418, 1795, 2162, 2729 |
| SmlI | CTYRAG | 5 cut(s) | 59, 165, 2230, 2236, 2335 |
| SmoI | CTYRAG | 5 cut(s) | 59, 165, 2230, 2236, 2335 |
| SpeI | ACTAGT | 2 cut(s) | 2459, 2554 |
| SphI | GCATGC | 4 cut(s) | 137, 790, 870, 1665 |
| SsiI | CCGC | 7 cut(s) | 488, 490, 539, 604, 606, 1976, 2429 |
| SspI | AATATT | 2 cut(s) | 1009, 2131 |
| SspMI | CTAG | 9 cut(s) | 51, 411, 1310, 1347, 1397, 1695, 2276, 2460, 2555 |
| SstI | GAGCTC | 2 cut(s) | 1598, 2237 |
| StyD4I | CCNGG | 8 cut(s) | 104, 581, 1036, 1078, 1242, 1341, 1677, 1855 |
| StyI | CCWWGG | 5 cut(s) | 519, 860, 2275, 2415, 2603 |
| TaaI | ACNGT | 3 cut(s) | 48, 930, 1451 |
| TaiI | ACGT | 2 cut(s) | 1177, 1462 |
| TaqI | TCGA | 6 cut(s) | 832, 1020, 1163, 1531, 2193, 2237 |
| TauI | GCSGC | 2 cut(s) | 490, 609 |
| TfiI | GAWTC | 8 cut(s) | 265, 680, 1373, 1438, 1724, 2203, 2282, 2640 |
| TscAI | CASTG | 2 cut(s) | 1032, 1546 |
| TseFI | GTSAC | 1 cut(s) | 708 |
| Tsp45I | GTSAC | 1 cut(s) | 708 |
| TspDTI | ATGAA | 8 cut(s) | 83, 1132, 1280, 1442, 1827, 1986, 2699, 2735 |
| TspRI | CASTG | 2 cut(s) | 1032, 1546 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 398 |
| VpaK11BI | GGWCC | 4 cut(s) | 102, 921, 1391, 2392 |
| XapI | RAATTY | 6 cut(s) | 118, 249, 441, 878, 899, 2612 |
| XceI | RCATGY | 7 cut(s) | 137, 790, 870, 1665, 1822, 2192, 2506 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 232, 932, 1835 |
| XhoI | CTCGAG | 1 cut(s) | 2236 |
| XmaJI | CCTAGG | 1 cut(s) | 2275 |
| XmiI | GTMKAC | 3 cut(s) | 55, 2193, 2365 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2644 |
| XspI | CTAG | 9 cut(s) | 51, 411, 1310, 1347, 1397, 1695, 2276, 2460, 2555 |
| Zsp2I | ATGCAT | 2 cut(s) | 1663, 1820 |