Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0016666

Physical Location & Seq

Genomic Coordinates Forward (+)

1684942 .. 1687641

Gene Structure 2700 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1023300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGAACCCCTTCGTCTTTCTTTTTCTCCTCCCAACTCTCCTCCACCTCTCCCACACCTGCCTCTCTTCTCCGCCGCAAACCGCCGCCTCCGAGCTCTTCGAAGCTTGGTGCAAACAGTATGGCAAGTCCTACCCCTCACCGCAAGAAAAGCTCCGCAGGCTCAGTGTGTTCGAGGGCAATCTCAACTTTGTCACCCGACACAACGAAATGGGGAATTCTTCTTATACCCTTTCTCTCAATGCGTTTTCTGATCTCACCCACAGCGAGTTCAAGGCCTCCCGATTAGGCTTCTCGCCCTCGTTCATCGGTCTTAAGCGTGAACCGGATAGAAAACCCGGTTTATTGGCTCGTGATGTTCCTTCTTCGATTGATTGGAGGAAGGAAGGGGCGGTGACGAAGGTTAAGGATCAAGGGAGTTGTGGTGCTTGTTGGGCATTCTCAACTACTGGTGCCATCGAAGGTATCAACAAAATTGTCACAGGTTCACTTCTCAGCCTTTCCGAACAAGAGCTGATTGATTGCGACAGAGTTTATCCAAACAGCGGCTGCAGTGGGGGACTTATGGACGATGCATTCGAATTTGTCATAGAAAACAATGGGGTCGACACTGAGGACGATTACCCGTATAAAGGTTCGGATGGCACCTGCAATAAGAAAAAGTTGAAGAGACATGCTGTGACCATAGATGATTATACTGATGTGCCGCCAAATGATGAGGAACAACTTCTACAAGCTGTGGCAAATCAGCCTGTGAGCGTTGGCATATGTGGCAGTGACATTGCATTTCAGTTGTACTCGAAGGGAATTTTCACTGGTCCGTGTTCGAATTCTTTGGATCACGCTGTTCTAATCGTAGGTTATGGTTCGGAAAATGGGGTGGACTATTGGATTGTGAAGAACTCATGGGGAAAAGGTTGGGGAATGAATGGCTACATCCACATGCTACGTGATCATAGCAATTCCCAAGGGGTCTGTGGCATCAACAGTTTAGCTTCATATCCGATAAAGACCGGGCAAAACCCTCCTCTTCCTCCGCCTTCTCCTCCCCCAGGTCCAACCAAATGCGACCTCTTTACGCACTGCGCAGCAGGGGAAACCTGCTGTTGTGCCAAGAGGTTTATTGGAATATGCTTTGCTTGGAGATGCTGTGAGTTGAACTCTGCTGTTTGTTGCAAGGATCACAATCACTGCTGTCCCCATGATTATCCCATCTGTGATACAGAAAGGGCGCTCTGCCTCAAGAGTAGCGAACTATCAACGCAATCCCAGGCTACTTCGAACGTAACAACAAAAGCGCTCATGGGCAGAAGCGTTTTGAGGAATTCGAGCCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

445

Amino Acids

48.55

Weight (kDa)

6.18

Isoelectric Point (pI)

38.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	33 - 90	1.4e-20	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	119 - 335	8.6e-84	Papain family cysteine protease
Granulin	PF00396	366 - 413	5.3e-09	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09850
fragaria_vesca	FvH4_2g38850
malus_domestica	MD08G1023300.v1.1
prunus_persica	Prupe.1G372500_v2.0.a1
pyrus_communis	pycom08g01810
rosa_chinensis	RchiOBHm_Chr6g0301951
rosa_laevigata	RLG00000011206
rosa_multiflora	Rmu_sc0000407.1_g000001
rosa_roxburghii	Rroxscaffold_7G00166250
rosa_rugosa	Rorug06G0310500
rosa_samantha	Rh6AG422900 Rh6BG429700 Rh6CG436100
rosa_wichuraiana	Rw6G036630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	65, 653
Acc16I	TGCGCA	1 cut(s)	1084
Acc36I	ACCTGC	3 cut(s)	65, 653, 1106
AccB1I	GGYRCC	2 cut(s)	449, 641
AccI	GTMKAC	1 cut(s)	603
AclWI	GGATC	3 cut(s)	414, 843, 1185
AcsI	RAATTY	5 cut(s)	214, 578, 804, 826, 1321
AfaI	GTAC	1 cut(s)	794
AfeI	AGCGCT	1 cut(s)	1296
AfiI	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
AflII	CTTAAG	1 cut(s)	311
AgsI	TTSAA	3 cut(s)	271, 664, 1156
AjnI	CCWGG	2 cut(s)	1048, 1266
AluBI	AGCT	6 cut(s)	94, 104, 151, 511, 734, 992
AluI	AGCT	6 cut(s)	94, 104, 151, 511, 734, 992
Alw21I	GWGCWC	1 cut(s)	96
Alw26I	GTCTC	1 cut(s)	661
AlwI	GGATC	3 cut(s)	414, 843, 1185
AlwNI	CAGNNNCTG	1 cut(s)	546
Aor51HI	AGCGCT	1 cut(s)	1296
AoxI	GGCC	1 cut(s)	273
ApeKI	GCWGC	2 cut(s)	546, 1085
ApoI	RAATTY	5 cut(s)	214, 578, 804, 826, 1321
ArsI	GACNNNNNNTTYG	2 cut(s)	557, 589
Asp700I	GAANNNNTTC	2 cut(s)	8, 723
AspLEI	GCGC	3 cut(s)	1085, 1231, 1297
AspS9I	GGNCC	2 cut(s)	815, 1052
AsuC2I	CCSGG	2 cut(s)	336, 1012
AsuHPI	GGTGA	4 cut(s)	129, 184, 247, 403
AsuII	TTCGAA	4 cut(s)	99, 576, 824, 1277
AvaII	GGWCC	2 cut(s)	815, 1052
BanI	GGYRCC	2 cut(s)	449, 641
BanII	GRGCYC	1 cut(s)	96
BauI	CACGAG	1 cut(s)	348
Bbv12I	GWGCWC	1 cut(s)	96
BbvI	GCAGC	2 cut(s)	533, 1097
BccI	CCATC	3 cut(s)	461, 632, 1217
BciT130I	CCWGG	2 cut(s)	1050, 1268
BclI	TGATCA	1 cut(s)	949
BcnI	CCSGG	2 cut(s)	336, 1012
BcoDI	GTCTC	1 cut(s)	661
BfmI	CTRYAG	1 cut(s)	547
BfoI	RGCGCY	2 cut(s)	1232, 1298
BfrI	CTTAAG	1 cut(s)	311
BfuAI	ACCTGC	3 cut(s)	65, 653, 1106
BisI	GCNGC	6 cut(s)	74, 84, 544, 547, 704, 1086
BlsI	GCNGC	6 cut(s)	75, 85, 545, 548, 705, 1087
Bme1390I	CCNGG	4 cut(s)	336, 1012, 1050, 1268
Bme18I	GGWCC	2 cut(s)	815, 1052
BmgT120I	GGNCC	2 cut(s)	815, 1052
BmiI	GGNNCC	2 cut(s)	451, 643
BmrFI	CCNGG	4 cut(s)	336, 1012, 1050, 1268
BmsI	GCATC	3 cut(s)	559, 987, 1133
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
Bpu14I	TTCGAA	4 cut(s)	99, 576, 824, 1277
BpuEI	CTTGAG	1 cut(s)	1223
BpuMI	CCSGG	2 cut(s)	336, 1012
BsaAI	YACGTR	1 cut(s)	947
BsaBI	GATNNNNATC	1 cut(s)	1182
BsaJI	CCNNGG	3 cut(s)	964, 1048, 1266
BsaWI	WCCGGW	1 cut(s)	322
BsaXI	ACNNNNNCTCC	4 cut(s)	406, 436, 1132, 1162
Bsc4I	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
Bse118I	RCCGGY	1 cut(s)	1329
Bse1I	ACTGG	2 cut(s)	451, 817
Bse3DI	GCAATG	1 cut(s)	777
Bse8I	GATNNNNATC	1 cut(s)	1182
BseBI	CCWGG	2 cut(s)	1050, 1268
BseDI	CCNNGG	3 cut(s)	964, 1048, 1266
BseGI	GGATG	2 cut(s)	643, 933
BseJI	GATNNNNATC	1 cut(s)	1182
BseLI	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
BseMI	GCAATG	1 cut(s)	777
BseMII	CTCAG	3 cut(s)	175, 505, 600
BseNI	ACTGG	2 cut(s)	451, 817
BseRI	GAGGAG	4 cut(s)	17, 29, 1014, 1032
BseXI	GCAGC	2 cut(s)	533, 1097
BshFI	GGCC	1 cut(s)	275
BshNI	GGYRCC	2 cut(s)	449, 641
BsiHKAI	GWGCWC	1 cut(s)	96
BsiSI	CCGG	4 cut(s)	323, 336, 1011, 1330
BslFI	GGGAC	2 cut(s)	570, 1179
BslI	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
BsmAI	GTCTC	1 cut(s)	661
BsmFI	GGGAC	2 cut(s)	570, 1179
BsmI	GAATGC	2 cut(s)	434, 572
BsnI	GGCC	1 cut(s)	275
Bsp119I	TTCGAA	4 cut(s)	99, 576, 824, 1277
Bsp1286I	GDGCHC	1 cut(s)	96
Bsp143I	GATC	5 cut(s)	250, 406, 835, 949, 1177
BspANI	GGCC	1 cut(s)	275
BspCNI	CTCAG	3 cut(s)	174, 504, 601
BspLI	GGNNCC	2 cut(s)	451, 643
BspMAI	CTGCAG	1 cut(s)	551
BspMI	ACCTGC	3 cut(s)	65, 653, 1106
BspPI	GGATC	3 cut(s)	414, 843, 1185
BspQI	GCTCTTC	1 cut(s)	101
BspT104I	TTCGAA	4 cut(s)	99, 576, 824, 1277
BspT107I	GGYRCC	2 cut(s)	449, 641
BspTI	CTTAAG	1 cut(s)	311
BsrDI	GCAATG	1 cut(s)	777
BsrFI	RCCGGY	1 cut(s)	1329
BsrI	ACTGG	2 cut(s)	451, 817
BssAI	RCCGGY	1 cut(s)	1329
BssECI	CCNNGG	3 cut(s)	964, 1048, 1266
BssMI	GATC	5 cut(s)	250, 406, 835, 949, 1177
BssSI	CACGAG	1 cut(s)	348
BssT1I	CCWWGG	1 cut(s)	964
Bst2BI	CACGAG	1 cut(s)	348
Bst2UI	CCWGG	2 cut(s)	1050, 1268
Bst4CI	ACNGT	2 cut(s)	117, 986
Bst6I	CTCTTC	4 cut(s)	70, 101, 659, 1032
BstAFI	CTTAAG	1 cut(s)	311
BstBAI	YACGTR	1 cut(s)	947
BstBI	TTCGAA	4 cut(s)	99, 576, 824, 1277
BstC8I	GCNNGC	1 cut(s)	158
BstDEI	CTNAG	3 cut(s)	161, 491, 609
BstENI	CCTNNNNNAGG	1 cut(s)	1047
BstF5I	GGATG	2 cut(s)	643, 933
BstH2I	RGCGCY	2 cut(s)	1232, 1298
BstHHI	GCGC	3 cut(s)	1085, 1231, 1297
BstKTI	GATC	5 cut(s)	253, 409, 838, 952, 1180
BstMAI	GTCTC	1 cut(s)	661
BstMBI	GATC	5 cut(s)	250, 406, 835, 949, 1177
BstMWI	GCNNNNNNNGC	4 cut(s)	148, 157, 431, 768
BstNI	CCWGG	2 cut(s)	1050, 1268
BstNSI	RCATGY	2 cut(s)	674, 943
BstSCI	CCNGG	4 cut(s)	334, 1010, 1048, 1266
BstSFI	CTRYAG	1 cut(s)	547
BstV1I	GCAGC	2 cut(s)	533, 1097
BsuRI	GGCC	1 cut(s)	275
BtsCI	GGATG	2 cut(s)	643, 933
BtsI	GCAGTG	4 cut(s)	556, 778, 1078, 1186
BtsIMutI	CAGTG	7 cut(s)	169, 556, 606, 778, 810, 1078, 1186
BveI	ACCTGC	3 cut(s)	65, 653, 1106
Cac8I	GCNNGC	1 cut(s)	158
CaiI	CAGNNNCTG	1 cut(s)	546
CfoI	GCGC	3 cut(s)	1085, 1231, 1297
Cfr10I	RCCGGY	1 cut(s)	1329
Cfr13I	GGNCC	2 cut(s)	815, 1052
Csp6I	GTAC	1 cut(s)	793
CviAII	CATG	5 cut(s)	671, 903, 940, 1199, 1300
CviQI	GTAC	1 cut(s)	793
DdeI	CTNAG	3 cut(s)	161, 491, 609
DpnI	GATC	5 cut(s)	252, 408, 837, 951, 1179
DpnII	GATC	5 cut(s)	250, 406, 835, 949, 1177
Eam1104I	CTCTTC	4 cut(s)	70, 101, 659, 1032
EarI	CTCTTC	4 cut(s)	70, 101, 659, 1032
EciI	GGCGGA	2 cut(s)	60, 1023
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	1 cut(s)	964
Eco147I	AGGCCT	1 cut(s)	275
Eco24I	GRGCYC	1 cut(s)	96
Eco47I	GGWCC	2 cut(s)	815, 1052
Eco47III	AGCGCT	1 cut(s)	1296
Eco53kI	GAGCTC	1 cut(s)	94
EcoICRI	GAGCTC	1 cut(s)	94
EcoNI	CCTNNNNNAGG	1 cut(s)	1047
EcoRI	GAATTC	3 cut(s)	214, 826, 1321
EcoRII	CCWGG	2 cut(s)	1048, 1266
EcoT14I	CCWWGG	1 cut(s)	964
EcoT22I	ATGCAT	1 cut(s)	574
EcoT38I	GRGCYC	1 cut(s)	96
ErhI	CCWWGG	1 cut(s)	964
FaeI	CATG	5 cut(s)	674, 906, 943, 1202, 1303
FaqI	GGGAC	2 cut(s)	570, 1179
FatI	CATG	5 cut(s)	670, 902, 939, 1198, 1299
FauNDI	CATATG	1 cut(s)	764
FbaI	TGATCA	1 cut(s)	949
FblI	GTMKAC	1 cut(s)	603
Fnu4HI	GCNGC	6 cut(s)	74, 84, 544, 547, 704, 1086
FokI	GGATG	2 cut(s)	650, 920
FriOI	GRGCYC	1 cut(s)	96
Fsp4HI	GCNGC	6 cut(s)	74, 84, 544, 547, 704, 1086
FspI	TGCGCA	1 cut(s)	1084
GlaI	GCGC	3 cut(s)	1084, 1230, 1296
GluI	GCNGC	6 cut(s)	74, 84, 544, 547, 704, 1086
HaeII	RGCGCY	2 cut(s)	1232, 1298
HaeIII	GGCC	1 cut(s)	275
HapII	CCGG	4 cut(s)	323, 336, 1011, 1330
HhaI	GCGC	3 cut(s)	1085, 1231, 1297
Hin1II	CATG	5 cut(s)	674, 906, 943, 1202, 1303
Hin6I	GCGC	3 cut(s)	1083, 1229, 1295
HinP1I	GCGC	3 cut(s)	1083, 1229, 1295
HincII	GTYRAC	1 cut(s)	604
HindII	GTYRAC	1 cut(s)	604
HindIII	AAGCTT	1 cut(s)	102
HpaII	CCGG	4 cut(s)	323, 336, 1011, 1330
HphI	GGTGA	4 cut(s)	129, 184, 247, 403
Hpy166II	GTNNAC	4 cut(s)	320, 485, 604, 880
Hpy188I	TCNGA	6 cut(s)	91, 250, 502, 637, 868, 1002
Hpy188III	TCNNGA	3 cut(s)	279, 350, 1240
Hpy8I	GTNNAC	4 cut(s)	320, 485, 604, 880
HpyAV	CCTTC	8 cut(s)	19, 369, 373, 377, 391, 452, 793, 1047
HpyCH4III	ACNGT	2 cut(s)	117, 986
HpyCH4IV	ACGT	2 cut(s)	946, 1281
HpyCH4V	TGCA	6 cut(s)	111, 549, 572, 648, 782, 1173
HpyF10VI	GCNNNNNNNGC	4 cut(s)	148, 157, 431, 768
HpyF3I	CTNAG	3 cut(s)	161, 491, 609
HpySE526I	ACGT	2 cut(s)	946, 1281
Hsp92II	CATG	5 cut(s)	674, 906, 943, 1202, 1303
HspAI	GCGC	3 cut(s)	1083, 1229, 1295
Ksp22I	TGATCA	1 cut(s)	949
Kzo9I	GATC	5 cut(s)	250, 406, 835, 949, 1177
LguI	GCTCTTC	1 cut(s)	101
LmnI	GCTCC	1 cut(s)	156
Lsp1109I	GCAGC	2 cut(s)	533, 1097
LweI	GCATC	3 cut(s)	559, 987, 1133
MaeII	ACGT	2 cut(s)	946, 1281
MaeIII	GTNAC	6 cut(s)	190, 391, 475, 676, 773, 1282
MalI	GATC	5 cut(s)	252, 408, 837, 951, 1179
MboI	GATC	5 cut(s)	250, 406, 835, 949, 1177
MboII	GAAGA	7 cut(s)	57, 88, 210, 354, 676, 907, 1019
MhlI	GDGCHC	1 cut(s)	96
MluCI	AATT	7 cut(s)	214, 471, 578, 804, 826, 958, 1321
MmeI	TCCRAC	1 cut(s)	1079
Mph1103I	ATGCAT	1 cut(s)	574
MroXI	GAANNNNTTC	2 cut(s)	8, 723
MseI	TTAA	2 cut(s)	312, 402
MslI	CAYNNNNRTG	1 cut(s)	938
MspA1I	CMGCKG	1 cut(s)	543
MspCI	CTTAAG	1 cut(s)	311
MspI	CCGG	4 cut(s)	323, 336, 1011, 1330
MspR9I	CCNGG	4 cut(s)	336, 1012, 1050, 1268
Mva1269I	GAATGC	2 cut(s)	434, 572
MvaI	CCWGG	2 cut(s)	1050, 1268
MwoI	GCNNNNNNNGC	4 cut(s)	148, 157, 431, 768
NciI	CCSGG	2 cut(s)	336, 1012
NdeI	CATATG	1 cut(s)	764
NdeII	GATC	5 cut(s)	250, 406, 835, 949, 1177
NlaIII	CATG	5 cut(s)	674, 906, 943, 1202, 1303
NlaIV	GGNNCC	2 cut(s)	451, 643
NmuCI	GTSAC	5 cut(s)	190, 391, 475, 676, 773
NsbI	TGCGCA	1 cut(s)	1084
NsiI	ATGCAT	1 cut(s)	574
NspI	RCATGY	2 cut(s)	674, 943
NspV	TTCGAA	4 cut(s)	99, 576, 824, 1277
PaqCI	CACCTGC	2 cut(s)	65, 653
PceI	AGGCCT	1 cut(s)	275
PciSI	GCTCTTC	1 cut(s)	101
PcsI	WCGNNNNNNNCGW	1 cut(s)	573
PctI	GAATGC	2 cut(s)	434, 572
PdmI	GAANNNNTTC	2 cut(s)	8, 723
PkrI	GCNGC	6 cut(s)	75, 85, 545, 548, 705, 1087
Ppu21I	YACGTR	1 cut(s)	947
Psp124BI	GAGCTC	1 cut(s)	96
Psp6I	CCWGG	2 cut(s)	1048, 1266
PspGI	CCWGG	2 cut(s)	1048, 1266
PspN4I	GGNNCC	2 cut(s)	451, 643
PspPI	GGNCC	2 cut(s)	815, 1052
PsrI	GAACNNNNNNTAC	2 cut(s)	711, 743
PstI	CTGCAG	1 cut(s)	551
PstNI	CAGNNNCTG	1 cut(s)	546
RsaI	GTAC	1 cut(s)	794
RsaNI	GTAC	1 cut(s)	793
RseI	CAYNNNNRTG	1 cut(s)	938
SacI	GAGCTC	1 cut(s)	96
SalI	GTCGAC	1 cut(s)	602
SapI	GCTCTTC	1 cut(s)	101
SaqAI	TTAA	2 cut(s)	312, 402
SatI	GCNGC	6 cut(s)	74, 84, 544, 547, 704, 1086
Sau3AI	GATC	5 cut(s)	250, 406, 835, 949, 1177
Sau96I	GGNCC	2 cut(s)	815, 1052
ScrFI	CCNGG	4 cut(s)	336, 1012, 1050, 1268
SduI	GDGCHC	1 cut(s)	96
SfaNI	GCATC	3 cut(s)	559, 987, 1133
SfcI	CTRYAG	1 cut(s)	547
SfuI	TTCGAA	4 cut(s)	99, 576, 824, 1277
SinI	GGWCC	2 cut(s)	815, 1052
SmiMI	CAYNNNNRTG	1 cut(s)	938
SmlI	CTYRAG	2 cut(s)	311, 1238
SmoI	CTYRAG	2 cut(s)	311, 1238
Sse9I	AATT	7 cut(s)	214, 471, 578, 804, 826, 958, 1321
SseBI	AGGCCT	1 cut(s)	275
SstI	GAGCTC	1 cut(s)	96
StuI	AGGCCT	1 cut(s)	275
StyD4I	CCNGG	4 cut(s)	334, 1010, 1048, 1266
StyI	CCWWGG	1 cut(s)	964
TaaI	ACNGT	2 cut(s)	117, 986
TaiI	ACGT	2 cut(s)	949, 1284
TaqII	GACCGA	1 cut(s)	296
TasI	AATT	7 cut(s)	214, 471, 578, 804, 826, 958, 1321
TatI	WGTACW	1 cut(s)	792
TauI	GCSGC	4 cut(s)	76, 86, 546, 706
Tru1I	TTAA	2 cut(s)	312, 402
Tru9I	TTAA	2 cut(s)	312, 402
TscAI	CASTG	7 cut(s)	169, 556, 613, 778, 817, 1085, 1193
TseFI	GTSAC	5 cut(s)	190, 391, 475, 676, 773
TseI	GCWGC	2 cut(s)	546, 1085
Tsp45I	GTSAC	5 cut(s)	190, 391, 475, 676, 773
TspDTI	ATGAA	4 cut(s)	17, 292, 938, 984
TspGWI	ACGGA	1 cut(s)	807
TspRI	CASTG	7 cut(s)	169, 556, 613, 778, 817, 1085, 1193
Vha464I	CTTAAG	1 cut(s)	311
VpaK11BI	GGWCC	2 cut(s)	815, 1052
XagI	CCTNNNNNAGG	1 cut(s)	1047
XapI	RAATTY	5 cut(s)	214, 578, 804, 826, 1321
XceI	RCATGY	2 cut(s)	674, 943
XcmI	CCANNNNNNNNNTGG	1 cut(s)	971
XmiI	GTMKAC	1 cut(s)	603
XmnI	GAANNNNTTC	2 cut(s)	8, 723
Zsp2I	ATGCAT	1 cut(s)	574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.