MD08G1023800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008721
Physical Location & Seq
Forward (+)
1708849 .. 1711538
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1023800.v1.1.491

Sequence Viewer

Length: 2670 bp
ATGGAGAATTTTACAATCCCCTGCAAATCAAGCCCTCCAACGGCCATCGTTCCCCCCAAATTTAGCAACCCATCTGAATTCTCACCACGACAAACCAAACCCACAATCTCTTTTTCCAGAAAAACCCTCCCAAAATCCACAGACTCTCACTTGAATTACCTCTGCAAGAATGGCGAGCTCACCAAGGCCGTCACGGTTCTTGAATCCATTGCCAAAAGTGGGTCGAAGGTAACTTCCACTACCTACATGAATTTACTTCAATCTTGCATTGACACCAATTGTATTCAACTGGGTCGGAAGATCCATGAGCGTATTGATATAGTTGAGGAATTGAACCCTTTTGTTGAAACGAAGTTGGTGAGTATGTATGCGAAATGCGGGTTTTTGGATGATGCGCGCAAGGTATTCTACGCAATGCGTGAGAGAAATTTGTACTCTTGGTCTGCCATGATCGGTGCGTGTGTCAGAGACCAGAGGTGGAAAGAGGTGGTGGAGCTTTTTTACTCGATGATGAGAGATGGGGTTTTGCCGGATTACTTTCTGTTTCCTAAGATATTGCAGGCTTGTGGGAATTGTAGGAATTTAGAGGCCACAAAATTGATACATTCCATTGTGGTTCGGTGTAACTTGAGTAGTTGTATTCAAGTGAACAATTCGATACTGGCAGTGTACGCAAAGTGTGGAAAATTGAAGTGGGCAAGGAGGTTTTTTGACGACATGGATGAGAAGGATGGGGTGAGTTGGAATGCAATTATATCTGGTTATTGCCACAAAGGTGAGACTGAAGAAGCTCAGAGACTGTTTGATGCGATGAGCAAAGAAGGGATTGAACCGGGGTTGGTAACTTGGAATACATTGATTGCTAGTCATAACCAGTTAGGGCATTGTGATGTCGCTATGGAACTGATGAGGAGGATGGAGAGCTGTGGGATAACTCCTGATGTATATACTTGGACTTCTATGATTTCGGGGTTTGCTCAAAACAACAGGAAAAATCAGTCTTTGGATTTGTTGAAGAAGATGCTTTTGGCAGGGGTACAACCAAATGGGATTACAATCACTAGTGCAATCTCAGCTTGCACATCTTTAAAATCACTCAACAAAGGGTTGGAAATCTATTCTATTGCTATAAAGATGGGTTTCATTGATGACGTACTGGTCGGAAATGCGCTCATTGGTATGTTTTCAAAATGTGGGGAACTGGAAGCTGCTCAGAAGGTCTTTGTTAGGATCCCAGAGAAAGATGTCTATACCTGGAACTCAATGATAGGAGGATATTGCCAAGCTAGATACTGTGGTAAGGCCTATGAACTATTCATGAAGATGCAGGAATCAGATGTGCATCCCAATGCTGTGACCTGGAATGTGATGATCACAGGATACATGCAGAGCGGAGATGCAGATCAAGCAATGGACCTCTTCCAAAGGATGGAAAAAGACGGGAAAGCTAAACGAAATACGGCATCTTGGAACTCTCTTGTCTCTGGCTACCTGCAACTTGGGGAGAAAGACAAGGCACTTGGGGTATTCAGGCAGATGCAGGCCTACTGTGTTAATCCCAATTCTGTTACTATATTGAGTGTCCTGCCTGCTTGTGCGAATTTAGTTGCCACGAAAAAGGTGAAAGAGATCCACGGTAGTGTATTGCGTAGAAATTTGGAGTCCTTAGTCCCTGTTGCAAATTCCTTGATAGACACCTATGCCAAATCGGGAAATATAGCATATTCAAGATTCATATTTGATAGAATGCCATCCAAAGATATCATCACTTGGAACTCGCTAATTTCTGGTTATGTCTTGCATGGTTTTTCAGACATTGCACTTGACCTTTTTGATCAGCTGAAGAAGTCGGGCTTTAAACGAAATAGAGGTACCTTTGCAAGTATCATATATGCATACAGTCTTGCTGGAATGGTAGACGAGGGGAGACAAGCTTTTTACAGCATCACTGAAGACTATCAAATCATACCAGGTCTAGAACATTATTCAGCTATGATAGATCTTTTCGGGCGTTCAGGTAGGCTTCAAGAAGCAATGCAGTTTGTTGAAGATATGCCCATAGAACCAGACTCCTCTATTTGGGCTGCCTTATTTACAGCATGTAGGATTCATGGGAATCTTGCCTTGGCAGTTCGTGTGGGGGAGCATTTACTCGACTTGGAACCAGGGAATATTTTGATTCAACAGTTACTGTTACAGGCATATGCACTATGTGGGAAGTCTGAGGATACTTCAAAATTGAGAAAGTTCGGTCGAGATGCTGCAATAAAGAAATTTACTGGGCAGTGCTGGATTGAGTTCAAGAACTCGGTGCATATGTACACTGCAGGTGACAGGTCGAAGTTATGCTCAAACTTTCTAAATTCGTGGTTACAAAACATAGACGAAAAAGCAAAGAGACCTGATTTTTGTAATGAGCTTTGTGTTGAGGAAGAAGAGGGGGGTATTAGCTGGGTCCATAGTGAAAAACTTGCATTTGCTTTTGCTCTCATTGGCTCCCCAAGTGTACCCCGAAGTATTAGGATGGTGAAAAATCTGAGAATGTGTGGAGACTGCCACAGGACAGCCAAGTATATATCTATGGCTTTTGGGTGTGATATATATTTGAGTGACCCAAAGTCGTTTCACCATTTTAGTAATGGCCATTGTTCTTGTGGTGACTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calreticulin DYW_deaminase Jacalin PPR PPR_2 PPR_3

Protein Analysis

890

Amino Acids

99.85

Weight (kDa)

8.32

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 244 - 287 2.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 244 - 282 2.7e-10 PPR repeat family
PPR_1 PF12854 244 - 271 1e-06 PPR repeat
PPR PF01535 246 - 276 1.7e-08 PPR repeat
PPR_2 PF13041 278 - 326 4.6e-12 PPR repeat family
PPR_2 PF13041 314 - 360 3.6e-08 PPR repeat family
PPR_2 PF13041 414 - 453 1.1e-09 PPR repeat family
PPR_1 PF12854 415 - 442 1e-05 PPR repeat
PPR PF01535 417 - 446 5.6e-08 PPR repeat
PPR_long PF17177 434 - 544 3.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 438 - 481 6.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 447 - 478 2e-06 PPR repeat
PPR_2 PF13041 449 - 481 1.4e-08 PPR repeat family
PPR PF01535 452 - 481 1.9e-08 PPR repeat
PPR_2 PF13041 486 - 534 2.4e-07 PPR repeat family
PPR_2 PF13041 586 - 633 1.2e-07 PPR repeat family
PPR PF01535 589 - 619 6.9e-07 PPR repeat
DYW_deaminase PF14432 809 - 889 3e-26 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2318
AasI GACNNNNNNGTC 1 cut(s) 1157
Acc36I ACCTGC 2 cut(s) 1500, 2318
Acc65I GGTACC 1 cut(s) 1871
AccB1I GGYRCC 1 cut(s) 1871
AccB7I CCANNNNNTGG 1 cut(s) 1429
AccBSI CCGCTC 1 cut(s) 1392
AccI GTMKAC 1 cut(s) 1917
AccII CGCG 1 cut(s) 397
AciI CCGC 2 cut(s) 378, 1392
AclWI GGATC 4 cut(s) 295, 1225, 1238, 1624
AcoI YGGCCR 2 cut(s) 42, 2641
AcuI CTGAAG 3 cut(s) 804, 1862, 1971
AdeI CACNNNGTG 1 cut(s) 2213
AfaI GTAC 7 cut(s) 434, 671, 1038, 1155, 1873, 2321, 2508
AfiI CCNNNNNNNGG 4 cut(s) 40, 219, 1429, 2079
AhdI GACNNNNNGTC 1 cut(s) 2617
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 4 cut(s) 1253, 1358, 1969, 2164
AjuI GAANNNNNNNTTGG 4 cut(s) 1010, 1042, 2108, 2140
AleI CACNNNNGTG 2 cut(s) 774, 1638
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 7 cut(s) 462, 773, 790, 1486, 1921, 2392, 2544
AlwI GGATC 4 cut(s) 295, 1225, 1238, 1624
AlwNI CAGNNNCTG 2 cut(s) 799, 2191
AoxI GGCC 6 cut(s) 42, 186, 588, 1302, 1542, 2641
ApeKI GCWGC 3 cut(s) 1208, 2084, 2261
ArsI GACNNNNNNTTYG 2 cut(s) 2391, 2423
Asp718I GGTACC 1 cut(s) 1871
AspLEI GCGC 3 cut(s) 397, 399, 1171
AspS9I GGNCC 2 cut(s) 1414, 2455
AsuC2I CCSGG 1 cut(s) 834
AvaII GGWCC 2 cut(s) 1414, 2455
BaeI ACNNNNGTAYC 2 cut(s) 1282, 1315
BalI TGGCCA 1 cut(s) 2643
BamHI GGATCC 1 cut(s) 1230
BanI GGYRCC 1 cut(s) 1871
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 1959
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 3 cut(s) 1195, 2071, 2248
BccI CCATC 9 cut(s) 53, 79, 512, 725, 910, 1129, 1423, 1759, 2518
BceAI ACGGC 3 cut(s) 57, 173, 1476
BciT130I CCWGG 4 cut(s) 1255, 1360, 1971, 2166
BciVI GTATCC 2 cut(s) 1373, 2221
BclI TGATCA 2 cut(s) 1371, 1834
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 7 cut(s) 462, 773, 790, 1486, 1921, 2392, 2544
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 4 cut(s) 864, 1062, 1287, 1976
BfmI CTRYAG 1 cut(s) 2325
BfuAI ACCTGC 2 cut(s) 1500, 2318
BfuI GTATCC 2 cut(s) 1373, 2221
BglII AGATCT 1 cut(s) 1999
BisI GCNGC 3 cut(s) 1209, 2085, 2262
BlsI GCNGC 3 cut(s) 1210, 2086, 2263
Bme1390I CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
Bme18I GGWCC 2 cut(s) 1414, 2455
BmeRI GACNNNNNGTC 1 cut(s) 2617
BmgT120I GGNCC 2 cut(s) 1414, 2455
BmiI GGNNCC 5 cut(s) 1232, 1873, 2163, 2456, 2497
BmrFI CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
BmrI ACTGGG 2 cut(s) 299, 2289
BmuI ACTGGG 2 cut(s) 299, 2289
BpiI GAAGAC 1 cut(s) 1959
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 1 cut(s) 834
BsaBI GATNNNNATC 3 cut(s) 1055, 1341, 1401
BsaI GGTCTC 2 cut(s) 462, 2392
BsaJI CCNNGG 5 cut(s) 183, 833, 1633, 2124, 2165
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 219, 1429, 2079
Bse1I ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2284
Bse3DI GCAATG 5 cut(s) 207, 420, 1416, 1815, 2040
Bse8I GATNNNNATC 3 cut(s) 1055, 1341, 1401
BseBI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
BseDI CCNNGG 5 cut(s) 183, 833, 1633, 2124, 2165
BseGI GGATG 8 cut(s) 394, 727, 736, 921, 1342, 1434, 1751, 2529
BseJI GATNNNNATC 3 cut(s) 1055, 1341, 1401
BseLI CCNNNNNNNGG 4 cut(s) 40, 219, 1429, 2079
BseMI GCAATG 5 cut(s) 207, 420, 1416, 1815, 2040
BseMII CTCAG 5 cut(s) 806, 1086, 1226, 2214, 2528
BseNI ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2284
BsePI GCGCGC 1 cut(s) 395
BseRI GAGGAG 2 cut(s) 925, 2062
BseXI GCAGC 3 cut(s) 1195, 2071, 2248
BseYI CCCAGC 1 cut(s) 2451
Bsh1236I CGCG 1 cut(s) 397
Bsh1285I CGRYCG 1 cut(s) 2254
BshFI GGCC 6 cut(s) 44, 188, 590, 1304, 1544, 2643
BshNI GGYRCC 1 cut(s) 1871
BsiEI CGRYCG 1 cut(s) 2254
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 2 cut(s) 530, 833
BslFI GGGAC 1 cut(s) 1655
BslI CCNNNNNNNGG 4 cut(s) 40, 219, 1429, 2079
BsmAI GTCTC 7 cut(s) 462, 773, 790, 1486, 1921, 2392, 2544
BsmFI GGGAC 1 cut(s) 1655
BsmI GAATGC 2 cut(s) 751, 1752
BsnI GGCC 6 cut(s) 44, 188, 590, 1304, 1544, 2643
Bso31I GGTCTC 2 cut(s) 462, 2392
Bsp1286I GDGCHC 1 cut(s) 180
Bsp1407I TGTACA 1 cut(s) 2319
Bsp143I GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
BspACI CCGC 2 cut(s) 378, 1392
BspANI GGCC 6 cut(s) 44, 188, 590, 1304, 1544, 2643
BspCNI CTCAG 5 cut(s) 805, 1085, 1225, 2215, 2529
BspFNI CGCG 1 cut(s) 397
BspHI TCATGA 1 cut(s) 1317
BspLI GGNNCC 5 cut(s) 1232, 1873, 2163, 2456, 2497
BspMAI CTGCAG 1 cut(s) 2329
BspMI ACCTGC 2 cut(s) 1500, 2318
BspPI GGATC 4 cut(s) 295, 1225, 1238, 1624
BspT107I GGYRCC 1 cut(s) 1871
BspTNI GGTCTC 2 cut(s) 462, 2392
BsrBI CCGCTC 1 cut(s) 1392
BsrDI GCAATG 5 cut(s) 207, 420, 1416, 1815, 2040
BsrGI TGTACA 1 cut(s) 2319
BsrI ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2284
BssECI CCNNGG 5 cut(s) 183, 833, 1633, 2124, 2165
BssHII GCGCGC 1 cut(s) 395
BssMI GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
BssT1I CCWWGG 2 cut(s) 183, 2124
Bst2UI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
Bst4CI ACNGT 8 cut(s) 196, 801, 1295, 1550, 1637, 1901, 2187, 2193
Bst6I CTCTTC 2 cut(s) 1424, 2430
BstAUI TGTACA 1 cut(s) 2319
BstC8I GCNNGC 6 cut(s) 176, 397, 561, 1078, 1542, 1590
BstDEI CTNAG 7 cut(s) 549, 792, 1072, 1212, 1666, 2223, 2537
BstDSI CCRYGG 1 cut(s) 1633
BstF5I GGATG 8 cut(s) 394, 727, 736, 921, 1342, 1434, 1751, 2529
BstFNI CGCG 1 cut(s) 397
BstHHI GCGC 3 cut(s) 397, 399, 1171
BstKTI GATC 8 cut(s) 303, 453, 1233, 1374, 1405, 1632, 1837, 2002
BstMAI GTCTC 7 cut(s) 462, 773, 790, 1486, 1921, 2392, 2544
BstMBI GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
BstMCI CGRYCG 1 cut(s) 2254
BstMWI GCNNNNNNNGC 5 cut(s) 30, 171, 671, 1073, 1406
BstNI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
BstNSI RCATGY 2 cut(s) 1387, 2103
BstSCI CCNGG 5 cut(s) 832, 1253, 1358, 1969, 2164
BstSFI CTRYAG 1 cut(s) 2325
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 1195, 2071, 2248
BstV2I GAAGAC 1 cut(s) 1959
BstX2I RGATCY 4 cut(s) 300, 1230, 1629, 1999
BstYI RGATCY 4 cut(s) 300, 1230, 1629, 1999
BsuI GTATCC 2 cut(s) 1373, 2221
BsuRI GGCC 6 cut(s) 44, 188, 590, 1304, 1544, 2643
BtgI CCRYGG 1 cut(s) 1633
BtgZI GCGATG 1 cut(s) 824
BtsCI GGATG 8 cut(s) 394, 727, 736, 921, 1342, 1434, 1751, 2529
BtsI GCAGTG 3 cut(s) 672, 2291, 2322
BtsIMutI CAGTG 4 cut(s) 672, 1947, 2291, 2322
BveI ACCTGC 2 cut(s) 1500, 2318
Cac8I GCNNGC 6 cut(s) 176, 397, 561, 1078, 1542, 1590
CaiI CAGNNNCTG 2 cut(s) 799, 2191
CciI TCATGA 1 cut(s) 1317
CfoI GCGC 3 cut(s) 397, 399, 1171
Cfr13I GGNCC 2 cut(s) 1414, 2455
CsiI ACCWGGT 1 cut(s) 1969
Csp6I GTAC 7 cut(s) 433, 670, 1037, 1154, 1872, 2320, 2507
CviAII CATG 9 cut(s) 247, 305, 448, 718, 1318, 1384, 1802, 2100, 2111
CviQI GTAC 7 cut(s) 433, 670, 1037, 1154, 1872, 2320, 2507
DdeI CTNAG 7 cut(s) 549, 792, 1072, 1212, 1666, 2223, 2537
DpnI GATC 8 cut(s) 302, 452, 1232, 1373, 1404, 1631, 1836, 2001
DpnII GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
DraI TTTAAA 2 cut(s) 1089, 1858
DraIII CACNNNGTG 1 cut(s) 2213
DrdI GACNNNNNNGTC 1 cut(s) 1157
DriI GACNNNNNGTC 1 cut(s) 2617
DseDI GACNNNNNNGTC 1 cut(s) 1157
EaeI YGGCCR 2 cut(s) 42, 2641
Eam1104I CTCTTC 2 cut(s) 1424, 2430
Eam1105I GACNNNNNGTC 1 cut(s) 2617
EarI CTCTTC 2 cut(s) 1424, 2430
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 183, 2124
Eco147I AGGCCT 2 cut(s) 1304, 1544
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 2 cut(s) 462, 2392
Eco32I GATATC 1 cut(s) 1762
Eco47I GGWCC 2 cut(s) 1414, 2455
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 3 cut(s) 804, 1862, 1971
EcoICRI GAGCTC 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 1253, 1358, 1969, 2164
EcoRV GATATC 1 cut(s) 1762
EcoT14I CCWWGG 2 cut(s) 183, 2124
EcoT22I ATGCAT 1 cut(s) 1897
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 183, 2124
FaeI CATG 9 cut(s) 250, 308, 451, 721, 1321, 1387, 1805, 2103, 2114
FalI AAGNNNNNCTT 2 cut(s) 1838, 1870
FaqI GGGAC 1 cut(s) 1655
FatI CATG 9 cut(s) 246, 304, 447, 717, 1317, 1383, 1801, 2099, 2110
FauI CCCGC 1 cut(s) 371
FauNDI CATATG 2 cut(s) 2203, 2316
FbaI TGATCA 2 cut(s) 1371, 1834
FblI GTMKAC 1 cut(s) 1917
Fnu4HI GCNGC 3 cut(s) 1209, 2085, 2262
FokI GGATG 8 cut(s) 401, 734, 743, 928, 1329, 1441, 1738, 2536
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 1209, 2085, 2262
FspBI CTAG 4 cut(s) 864, 1062, 1287, 1976
GlaI GCGC 3 cut(s) 396, 398, 1170
GluI GCNGC 3 cut(s) 1209, 2085, 2262
GsaI CCCAGC 1 cut(s) 2455
HaeIII GGCC 6 cut(s) 44, 188, 590, 1304, 1544, 2643
HapII CCGG 2 cut(s) 530, 833
HhaI GCGC 3 cut(s) 397, 399, 1171
Hin1II CATG 9 cut(s) 250, 308, 451, 721, 1321, 1387, 1805, 2103, 2114
Hin6I GCGC 3 cut(s) 395, 397, 1169
HinP1I GCGC 3 cut(s) 395, 397, 1169
HindIII AAGCTT 1 cut(s) 1932
HinfI GANTC 9 cut(s) 143, 203, 1331, 1661, 1731, 2069, 2107, 2116, 2179
HpaII CCGG 2 cut(s) 530, 833
Hpy166II GTNNAC 5 cut(s) 649, 670, 1918, 2322, 2507
Hpy8I GTNNAC 5 cut(s) 649, 670, 1918, 2322, 2507
HpyAV CCTTC 4 cut(s) 220, 721, 815, 1210
HpyCH4III ACNGT 8 cut(s) 196, 801, 1295, 1550, 1637, 1901, 2187, 2193
HpyCH4IV ACGT 1 cut(s) 1152
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 171, 671, 1073, 1406
HpyF3I CTNAG 7 cut(s) 549, 792, 1072, 1212, 1666, 2223, 2537
HpySE526I ACGT 1 cut(s) 1152
Hsp92II CATG 9 cut(s) 250, 308, 451, 721, 1321, 1387, 1805, 2103, 2114
HspAI GCGC 3 cut(s) 395, 397, 1169
KpnI GGTACC 1 cut(s) 1875
Ksp22I TGATCA 2 cut(s) 1371, 1834
Kzo9I GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
LmnI GCTCC 3 cut(s) 493, 2143, 2501
Lsp1109I GCAGC 3 cut(s) 1195, 2071, 2248
MabI ACCWGGT 1 cut(s) 1969
MaeI CTAG 4 cut(s) 864, 1062, 1287, 1976
MaeII ACGT 1 cut(s) 1152
MalI GATC 8 cut(s) 302, 452, 1232, 1373, 1404, 1631, 1836, 2001
MbiI CCGCTC 1 cut(s) 1392
MboI GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
MfeI CAATTG 1 cut(s) 277
MflI RGATCY 4 cut(s) 300, 1230, 1629, 1999
MhlI GDGCHC 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 2643
MluNI TGGCCA 1 cut(s) 2643
MlyI GAGTC 3 cut(s) 137, 1670, 2063
MmeI TCCRAC 5 cut(s) 62, 275, 722, 1089, 1141
Mox20I TGGCCA 1 cut(s) 2643
Mph1103I ATGCAT 1 cut(s) 1897
MscI TGGCCA 1 cut(s) 2643
MseI TTAA 3 cut(s) 1088, 1554, 1857
MslI CAYNNNNRTG 6 cut(s) 774, 888, 1178, 1322, 1347, 1638
Msp20I TGGCCA 1 cut(s) 2643
MspA1I CMGCKG 1 cut(s) 1840
MspI CCGG 2 cut(s) 530, 833
MspR9I CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
MunI CAATTG 1 cut(s) 277
Mva1269I GAATGC 2 cut(s) 751, 1752
MvaI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 5 cut(s) 30, 171, 671, 1073, 1406
NciI CCSGG 1 cut(s) 834
NdeI CATATG 2 cut(s) 2203, 2316
NdeII GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
NlaIII CATG 9 cut(s) 250, 308, 451, 721, 1321, 1387, 1805, 2103, 2114
NlaIV GGNNCC 5 cut(s) 1232, 1873, 2163, 2456, 2497
NmuCI GTSAC 5 cut(s) 190, 1354, 2330, 2609, 2657
NsiI ATGCAT 1 cut(s) 1897
NspI RCATGY 2 cut(s) 1387, 2103
OliI CACNNNNGTG 2 cut(s) 774, 1638
PagI TCATGA 1 cut(s) 1317
PaqCI CACCTGC 1 cut(s) 2318
PauI GCGCGC 1 cut(s) 395
PceI AGGCCT 2 cut(s) 1304, 1544
PctI GAATGC 2 cut(s) 751, 1752
PfeI GAWTC 6 cut(s) 203, 1331, 1731, 2107, 2116, 2179
PflMI CCANNNNNTGG 1 cut(s) 1429
PkrI GCNGC 3 cut(s) 1210, 2086, 2263
PleI GAGTC 3 cut(s) 137, 1669, 2063
PpsI GAGTC 3 cut(s) 137, 1669, 2063
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 4 cut(s) 1253, 1358, 1969, 2164
PspFI CCCAGC 1 cut(s) 2451
PspGI CCWGG 4 cut(s) 1253, 1358, 1969, 2164
PspN4I GGNNCC 5 cut(s) 1232, 1873, 2163, 2456, 2497
PspPI GGNCC 2 cut(s) 1414, 2455
PstI CTGCAG 1 cut(s) 2329
PstNI CAGNNNCTG 2 cut(s) 799, 2191
PsuI RGATCY 4 cut(s) 300, 1230, 1629, 1999
PteI GCGCGC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 1840
RsaI GTAC 7 cut(s) 434, 671, 1038, 1155, 1873, 2321, 2508
RsaNI GTAC 7 cut(s) 433, 670, 1037, 1154, 1872, 2320, 2507
RseI CAYNNNNRTG 6 cut(s) 774, 888, 1178, 1322, 1347, 1638
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 1088, 1554, 1857
SatI GCNGC 3 cut(s) 1209, 2085, 2262
Sau3AI GATC 8 cut(s) 300, 450, 1230, 1371, 1402, 1629, 1834, 1999
Sau96I GGNCC 2 cut(s) 1414, 2455
SchI GAGTC 3 cut(s) 137, 1670, 2063
ScrFI CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
SduI GDGCHC 1 cut(s) 180
SexAI ACCWGGT 1 cut(s) 1969
SfcI CTRYAG 1 cut(s) 2325
SinI GGWCC 2 cut(s) 1414, 2455
SmiMI CAYNNNNRTG 6 cut(s) 774, 888, 1178, 1322, 1347, 1638
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
SpeI ACTAGT 1 cut(s) 1061
SseBI AGGCCT 2 cut(s) 1304, 1544
SsiI CCGC 2 cut(s) 378, 1392
SspI AATATT 1 cut(s) 2173
SspMI CTAG 4 cut(s) 864, 1062, 1287, 1976
SstI GAGCTC 1 cut(s) 180
StuI AGGCCT 2 cut(s) 1304, 1544
StyD4I CCNGG 5 cut(s) 832, 1253, 1358, 1969, 2164
StyI CCWWGG 2 cut(s) 183, 2124
TaaI ACNGT 8 cut(s) 196, 801, 1295, 1550, 1637, 1901, 2187, 2193
TaiI ACGT 1 cut(s) 1155
TaqI TCGA 6 cut(s) 224, 506, 656, 2154, 2254, 2339
TaqII GACCGA 1 cut(s) 2240
TatI WGTACW 2 cut(s) 432, 2319
TfiI GAWTC 6 cut(s) 203, 1331, 1731, 2107, 2116, 2179
Tru1I TTAA 3 cut(s) 1088, 1554, 1857
Tru9I TTAA 3 cut(s) 1088, 1554, 1857
TscAI CASTG 4 cut(s) 672, 1954, 2291, 2329
TseFI GTSAC 5 cut(s) 190, 1354, 2330, 2609, 2657
TseI GCWGC 3 cut(s) 1208, 2084, 2261
Tsp45I GTSAC 5 cut(s) 190, 1354, 2330, 2609, 2657
TspDTI ATGAA 7 cut(s) 263, 1132, 1306, 1323, 1334, 1723, 2099
TspRI CASTG 4 cut(s) 672, 1954, 2291, 2329
Van91I CCANNNNNTGG 1 cut(s) 1429
VpaK11BI GGWCC 2 cut(s) 1414, 2455
XbaI TCTAGA 1 cut(s) 1975
XceI RCATGY 2 cut(s) 1387, 2103
XcmI CCANNNNNNNNNTGG 2 cut(s) 2636, 2651
XmiI GTMKAC 1 cut(s) 1917
XspI CTAG 4 cut(s) 864, 1062, 1287, 1976
Zsp2I ATGCAT 1 cut(s) 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.