pycom08g01860

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008721
Physical Location & Seq
Forward (+)
1371789 .. 1374461
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01860.1

Sequence Viewer

Length: 2673 bp
ATGGAGAATTTTACAATCCCCTGCAAATCAAACCCTCCAACAGCCATCATTCCCCCCAAATTTAGCAACCCATCTGAATTCTCACCACGACAAACCAAACCGACAATCTCTTTTTCCAGAAAAACCCTCCCAAAATCCACAGACTCTCACTTGAATTACCTCTGCAAGAATGGCGAGCTCACTAAGGCCGTCACGGTTCTTGAATCCATTGCCAAAAGTGGGTCGAAGGTAACTTCCACTACCTACATGAATTTACTTCAATCTTGCATTGACACCAATTCTATTCAACTGGGTCGGAAGATCCATGAGCGTATTGATGTAGTTGAGGAATTGAACCCTTTTGTTGAAACGAAGTTGGTGAGTATGTATGCGAAATGCGGGTTTTTGGATGATGCGCGCAAGGTATTCTGCGCAATGCGTGAGAGAAATTTGTACTCTTGGTCTGCCATGATTGGTGCGTGTCTCAGAGACCAGAGGTGGAAAGAGGTGGTGGAGCTTTTTTACTCAATGATGAGAGATGGGGTTTTGCCGGATTACTTTCTGTTTCCTAAGATATTGCAGGCTTGTGGGAATTGTAGGAATTTAGAGGCCACAAAATTGATACATTCCATTGTGGTTCGGTGTAACTTGAGTAGTTGTATTCAAGTGAACAATTCGATACTGGCAGTGTACGCAAAGTGTGGAAAATTGAAGTGGGCAAGGAGGTTTTTCGACGACATGGATGAGAAGGATGGGGTGAGTTGGAATGCAATTATATCTGGTTATTGCCACAAAGGTGAGACTGAAGAAGCTCAGAGACTGTTTGATGCGATGAGCAAAGAAGGGATTGAACCGGGGTTGGTAACTTGGAATACATTGATTGCTAGTCATAACCAGTTAGGGCATTGTGATGTCGCTATGGAACTGATGAGGAGGATGGAGAGCTGTGGGATAACTCCTGATGTATATACTTGGACTTCTATGATTTCGGGGTTTGCTCAAAACAACAGGAAAAATCAGTCTTTGGATTTGTTGAAGAAGATGCTTTTGGCAGGGGTACAACCAAATGGGATTACAATCACTAGTGCAATCTCAGCTTGCACATCTTTAAAATCACTCCACAAAGGGTTGGAAATCTATTCTATTGCTATAAAGATGGGTTTCATTGATGACGTACTGGTCGGAAATGCACTCATTGGTATGTTTTCAAAATGTGGGGAACTGGAAGCTGCTCAGAAGGTCTTTGTTAGGATCGATCCAGAGAAAGATGTCTATACCTGGAACTCAATGATAGGAGGATATTGCCAAGCTAGATACTGTGGTAAGGCCTATGAACTGTTCATGAAGATGCAGGAATCAGATGTGCATCCCAATGCTGTGACCTGGAATGTGATGATCACAGGATACATGCAGAGCGGAGATGCAGATCAAGCAATGGACCTCTTCCAAGGGATGGAAAAAGACGGGAAAGTTAAACGAAATACGGCATCTTGGAACTCTCTTGTCTCTGGCTACCTGCAACTTGGGGAGAAAGACAAGGCACTTGGGGTATTCAGGCAGATGCAGGCCTACTGTGTTAATCCCAATTCTGTTACTATATTGAGTGTCCTGCCTGCTTGTGCGAATTTAGTTGCCACGAAAAAGGTGAAAGAGATCCATGGTAGTGTATTGCGTAGGAATTTGGAGTCCGTAGTCCCTGTTGCAAATTCCTTGATAGACACCTATGCCAAATCGGGAAATATAGCATATTCAAGATTCATATTTGATAGAATGCCATCCAAAGATATCATCACTTGGAACTCGCTAATTTCTGGTTATGTCTTGCATGGTCTTTCAGACATTGCACTTGACCTTTTTGATCAGCTGAAGAAGTCGGGCTTTAAACGAAATAGAGGTACCTTTGCAAGTATCATATATGCATACAGTCTTGCTGGAATGGTAGACGAGGGGAGACAAGCTTTTTACAGCATCACTGAAGACTATCAAATCATACCAGGTCTAGAACATTATTCAGCTATGATAGATCTTTTTGGGCGTTCAGGTAGGCTTCAAGAAGCAATGCAGTTTGTTGAAGATATGCCTATAGAACCAGACTCCTCTATTTGGGCTGCCTTATTTACAGCATGTAGGATTCATGGAAATCTTGCCTTGGCAGTTCGTGTGGGGGAGCATTTACTCGACTTGGAACCAGGGAATATTTTGATTCAACAGTTACTGTTACAGGCATATGCTCTGTGTGGGATATCTGAGGATACTTCAAAATTGAGAAAGTTTGGTCGAGATGCCGCAATAAAGAAATTTACTGGGCAGTGCTGGATTGAGTTCAAGAACTCAGTGCATACGTACACTGCAGGTGACAGGTCGAAGTTATGCTCAAACTTTCTTAATTCATGGTTACAAAACATAGACGAAAAAGCAAAGAGACCTGATTTTTGTAATGAGCTTTGTGTTGAGGAAGAAGAGGGGGGTATTAGCTGGGTCCATAGTGAAAAACTTGCATTTGCTTTTGCTCTCATTGGCTCCCCAAGTGTACCCCGAAGTATTAGGATGGTGAAAAATCTGAGAATGTGTGGAGACTGCCACAGGACAGCCAAGTATATATCTATGGCTTTTGGGTGTGATATATATTTGAGTGACCCAAAGTCGTTTCACCATTTTAGTAATGGTCATTGTTCTTGTGGTGACTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calreticulin DYW_deaminase Jacalin PPR PPR_2 PPR_3

Protein Analysis

891

Amino Acids

99.79

Weight (kDa)

8.07

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 244 - 282 2.7e-10 PPR repeat family
PPR_3 PF13812 244 - 287 2.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 244 - 271 1e-06 PPR repeat
PPR PF01535 246 - 276 1.7e-08 PPR repeat
PPR_2 PF13041 278 - 326 4.6e-12 PPR repeat family
PPR_2 PF13041 314 - 360 3.7e-08 PPR repeat family
PPR_3 PF13812 414 - 460 9.3e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 415 - 461 2.5e-13 PPR repeat family
PPR_1 PF12854 416 - 443 9.3e-06 PPR repeat
PPR PF01535 418 - 447 5.6e-08 PPR repeat
PPR_long PF17177 435 - 548 1.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 448 - 479 1e-05 PPR repeat
PPR_2 PF13041 450 - 482 6.2e-08 PPR repeat family
PPR PF01535 453 - 482 1.8e-07 PPR repeat
PPR_2 PF13041 487 - 535 2.4e-07 PPR repeat family
PPR_2 PF13041 587 - 634 2.1e-08 PPR repeat family
PPR PF01535 590 - 620 4.4e-07 PPR repeat
DYW_deaminase PF14432 810 - 890 3e-26 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2321
AasI GACNNNNNNGTC 1 cut(s) 1157
Acc16I TGCGCA 1 cut(s) 412
Acc36I ACCTGC 2 cut(s) 1503, 2321
Acc65I GGTACC 1 cut(s) 1874
AccB1I GGYRCC 1 cut(s) 1874
AccB7I CCANNNNNTGG 1 cut(s) 1432
AccBSI CCGCTC 1 cut(s) 1395
AccI GTMKAC 1 cut(s) 1920
AccII CGCG 1 cut(s) 397
AciI CCGC 3 cut(s) 378, 1395, 2265
AclWI GGATC 4 cut(s) 295, 1229, 1238, 1627
AcuI CTGAAG 3 cut(s) 804, 1865, 1974
AfaI GTAC 7 cut(s) 434, 671, 1038, 1155, 1876, 2324, 2511
AfiI CCNNNNNNNGG 3 cut(s) 219, 1432, 2082
AhdI GACNNNNNGTC 1 cut(s) 2620
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 4 cut(s) 1256, 1361, 1972, 2167
AjuI GAANNNNNNNTTGG 2 cut(s) 1010, 1042
AleI CACNNNNGTG 1 cut(s) 774
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 8 cut(s) 462, 467, 773, 790, 1489, 1924, 2395, 2547
AlwI GGATC 4 cut(s) 295, 1229, 1238, 1627
AlwNI CAGNNNCTG 2 cut(s) 799, 2194
AoxI GGCC 4 cut(s) 186, 588, 1305, 1545
ApeKI GCWGC 2 cut(s) 1208, 2087
ArsI GACNNNNNNTTYG 2 cut(s) 2394, 2426
Asp718I GGTACC 1 cut(s) 1874
AspLEI GCGC 3 cut(s) 397, 399, 413
AspS9I GGNCC 2 cut(s) 1417, 2458
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 9 cut(s) 75, 370, 748, 788, 1636, 2345, 2542, 2621, 2672
AvaII GGWCC 2 cut(s) 1417, 2458
BaeI ACNNNNGTAYC 2 cut(s) 1285, 1318
BanI GGYRCC 1 cut(s) 1874
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 1962
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 1195, 2074
BccI CCATC 9 cut(s) 53, 79, 512, 725, 910, 1129, 1426, 1762, 2521
BceAI ACGGC 2 cut(s) 173, 1479
BciT130I CCWGG 4 cut(s) 1258, 1363, 1974, 2169
BciVI GTATCC 2 cut(s) 1376, 2224
BclI TGATCA 2 cut(s) 1374, 1837
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 8 cut(s) 462, 467, 773, 790, 1489, 1924, 2395, 2547
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 4 cut(s) 864, 1062, 1290, 1979
BfmI CTRYAG 2 cut(s) 2061, 2328
BfuAI ACCTGC 2 cut(s) 1503, 2321
BfuI GTATCC 2 cut(s) 1376, 2224
BglII AGATCT 1 cut(s) 2002
BisI GCNGC 3 cut(s) 1209, 2088, 2265
BlsI GCNGC 3 cut(s) 1210, 2089, 2266
Bme1390I CCNGG 5 cut(s) 834, 1258, 1363, 1974, 2169
Bme18I GGWCC 2 cut(s) 1417, 2458
BmeRI GACNNNNNGTC 1 cut(s) 2620
BmgT120I GGNCC 2 cut(s) 1417, 2458
BmiI GGNNCC 4 cut(s) 1876, 2166, 2459, 2500
BmrFI CCNGG 5 cut(s) 834, 1258, 1363, 1974, 2169
BmrI ACTGGG 2 cut(s) 299, 2292
BmuI ACTGGG 2 cut(s) 299, 2292
BpiI GAAGAC 1 cut(s) 1962
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 1 cut(s) 834
Bsa29I ATCGAT 1 cut(s) 1233
BsaAI YACGTR 1 cut(s) 2322
BsaBI GATNNNNATC 3 cut(s) 1055, 1344, 1404
BsaI GGTCTC 2 cut(s) 462, 2395
BsaJI CCNNGG 5 cut(s) 833, 1426, 1636, 2127, 2168
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 1432, 2082
Bse1I ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2287
Bse3DI GCAATG 5 cut(s) 207, 420, 1419, 1818, 2043
Bse8I GATNNNNATC 3 cut(s) 1055, 1344, 1404
BseBI CCWGG 4 cut(s) 1258, 1363, 1974, 2169
BseCI ATCGAT 1 cut(s) 1233
BseDI CCNNGG 5 cut(s) 833, 1426, 1636, 2127, 2168
BseGI GGATG 8 cut(s) 394, 727, 736, 921, 1345, 1437, 1754, 2532
BseJI GATNNNNATC 3 cut(s) 1055, 1344, 1404
BseLI CCNNNNNNNGG 3 cut(s) 219, 1432, 2082
BseMI GCAATG 5 cut(s) 207, 420, 1419, 1818, 2043
BseMII CTCAG 7 cut(s) 478, 806, 1086, 1226, 2217, 2325, 2531
BseNI ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2287
BsePI GCGCGC 1 cut(s) 395
BseRI GAGGAG 2 cut(s) 925, 2065
BseXI GCAGC 2 cut(s) 1195, 2074
BseYI CCCAGC 1 cut(s) 2454
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 4 cut(s) 188, 590, 1307, 1547
BshNI GGYRCC 1 cut(s) 1874
BshVI ATCGAT 1 cut(s) 1233
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 2 cut(s) 530, 833
BslFI GGGAC 1 cut(s) 1658
BslI CCNNNNNNNGG 3 cut(s) 219, 1432, 2082
BsmAI GTCTC 8 cut(s) 462, 467, 773, 790, 1489, 1924, 2395, 2547
BsmFI GGGAC 1 cut(s) 1658
BsmI GAATGC 2 cut(s) 751, 1755
BsnI GGCC 4 cut(s) 188, 590, 1307, 1547
Bso31I GGTCTC 2 cut(s) 462, 2395
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
Bsp19I CCATGG 1 cut(s) 1636
BspACI CCGC 3 cut(s) 378, 1395, 2265
BspANI GGCC 4 cut(s) 188, 590, 1307, 1547
BspCNI CTCAG 7 cut(s) 477, 805, 1085, 1225, 2218, 2324, 2532
BspDI ATCGAT 1 cut(s) 1233
BspFNI CGCG 1 cut(s) 397
BspHI TCATGA 1 cut(s) 1320
BspLI GGNNCC 4 cut(s) 1876, 2166, 2459, 2500
BspMAI CTGCAG 1 cut(s) 2332
BspMI ACCTGC 2 cut(s) 1503, 2321
BspPI GGATC 4 cut(s) 295, 1229, 1238, 1627
BspT107I GGYRCC 1 cut(s) 1874
BspTNI GGTCTC 2 cut(s) 462, 2395
BsrBI CCGCTC 1 cut(s) 1395
BsrDI GCAATG 5 cut(s) 207, 420, 1419, 1818, 2043
BsrI ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2287
BssECI CCNNGG 5 cut(s) 833, 1426, 1636, 2127, 2168
BssHII GCGCGC 1 cut(s) 395
BssMI GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
BssT1I CCWWGG 3 cut(s) 1426, 1636, 2127
Bst2UI CCWGG 4 cut(s) 1258, 1363, 1974, 2169
Bst4CI ACNGT 8 cut(s) 196, 801, 1298, 1317, 1553, 1904, 2190, 2196
Bst6I CTCTTC 2 cut(s) 1427, 2433
BstBAI YACGTR 1 cut(s) 2322
BstC8I GCNNGC 6 cut(s) 176, 397, 561, 1078, 1545, 1593
BstDEI CTNAG 9 cut(s) 183, 464, 549, 792, 1072, 1212, 2226, 2311, 2540
BstDSI CCRYGG 1 cut(s) 1636
BstF5I GGATG 8 cut(s) 394, 727, 736, 921, 1345, 1437, 1754, 2532
BstFNI CGCG 1 cut(s) 397
BstHHI GCGC 3 cut(s) 397, 399, 413
BstKTI GATC 8 cut(s) 303, 1233, 1237, 1377, 1408, 1635, 1840, 2005
BstMAI GTCTC 8 cut(s) 462, 467, 773, 790, 1489, 1924, 2395, 2547
BstMBI GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
BstMWI GCNNNNNNNGC 4 cut(s) 171, 671, 1073, 1409
BstNI CCWGG 4 cut(s) 1258, 1363, 1974, 2169
BstNSI RCATGY 2 cut(s) 1390, 2106
BstSCI CCNGG 5 cut(s) 832, 1256, 1361, 1972, 2167
BstSFI CTRYAG 2 cut(s) 2061, 2328
BstSNI TACGTA 1 cut(s) 2322
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 2 cut(s) 1195, 2074
BstV2I GAAGAC 1 cut(s) 1962
BstX2I RGATCY 3 cut(s) 300, 1632, 2002
BstYI RGATCY 3 cut(s) 300, 1632, 2002
Bsu15I ATCGAT 1 cut(s) 1233
BsuI GTATCC 2 cut(s) 1376, 2224
BsuRI GGCC 4 cut(s) 188, 590, 1307, 1547
BsuTUI ATCGAT 1 cut(s) 1233
BtgI CCRYGG 1 cut(s) 1636
BtgZI GCGATG 1 cut(s) 824
BtsCI GGATG 8 cut(s) 394, 727, 736, 921, 1345, 1437, 1754, 2532
BtsI GCAGTG 3 cut(s) 672, 2294, 2325
BtsIMutI CAGTG 5 cut(s) 672, 1950, 2294, 2319, 2325
BveI ACCTGC 2 cut(s) 1503, 2321
Cac8I GCNNGC 6 cut(s) 176, 397, 561, 1078, 1545, 1593
CaiI CAGNNNCTG 2 cut(s) 799, 2194
CciI TCATGA 1 cut(s) 1320
CfoI GCGC 3 cut(s) 397, 399, 413
Cfr13I GGNCC 2 cut(s) 1417, 2458
ClaI ATCGAT 1 cut(s) 1233
CsiI ACCWGGT 1 cut(s) 1972
Csp6I GTAC 7 cut(s) 433, 670, 1037, 1154, 1875, 2323, 2510
CviQI GTAC 7 cut(s) 433, 670, 1037, 1154, 1875, 2323, 2510
DdeI CTNAG 9 cut(s) 183, 464, 549, 792, 1072, 1212, 2226, 2311, 2540
DpnI GATC 8 cut(s) 302, 1232, 1236, 1376, 1407, 1634, 1839, 2004
DpnII GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
DraI TTTAAA 2 cut(s) 1089, 1861
DrdI GACNNNNNNGTC 1 cut(s) 1157
DriI GACNNNNNGTC 1 cut(s) 2620
DseDI GACNNNNNNGTC 1 cut(s) 1157
Eam1104I CTCTTC 2 cut(s) 1427, 2433
Eam1105I GACNNNNNGTC 1 cut(s) 2620
EarI CTCTTC 2 cut(s) 1427, 2433
Ecl136II GAGCTC 1 cut(s) 178
Eco105I TACGTA 1 cut(s) 2322
Eco130I CCWWGG 3 cut(s) 1426, 1636, 2127
Eco147I AGGCCT 2 cut(s) 1307, 1547
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 2 cut(s) 462, 2395
Eco32I GATATC 2 cut(s) 1765, 2223
Eco47I GGWCC 2 cut(s) 1417, 2458
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 3 cut(s) 804, 1865, 1974
EcoICRI GAGCTC 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 1256, 1361, 1972, 2167
EcoRV GATATC 2 cut(s) 1765, 2223
EcoT14I CCWWGG 3 cut(s) 1426, 1636, 2127
EcoT22I ATGCAT 1 cut(s) 1900
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 3 cut(s) 1426, 1636, 2127
FalI AAGNNNNNCTT 2 cut(s) 1841, 1873
FaqI GGGAC 1 cut(s) 1658
FauI CCCGC 1 cut(s) 371
FauNDI CATATG 1 cut(s) 2206
FbaI TGATCA 2 cut(s) 1374, 1837
FblI GTMKAC 1 cut(s) 1920
Fnu4HI GCNGC 3 cut(s) 1209, 2088, 2265
FokI GGATG 8 cut(s) 401, 734, 743, 928, 1332, 1444, 1741, 2539
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 1209, 2088, 2265
FspBI CTAG 4 cut(s) 864, 1062, 1290, 1979
FspI TGCGCA 1 cut(s) 412
GlaI GCGC 3 cut(s) 396, 398, 412
GluI GCNGC 3 cut(s) 1209, 2088, 2265
GsaI CCCAGC 1 cut(s) 2458
HaeIII GGCC 4 cut(s) 188, 590, 1307, 1547
HapII CCGG 2 cut(s) 530, 833
HhaI GCGC 3 cut(s) 397, 399, 413
Hin6I GCGC 3 cut(s) 395, 397, 411
HinP1I GCGC 3 cut(s) 395, 397, 411
HindIII AAGCTT 1 cut(s) 1935
HinfI GANTC 8 cut(s) 143, 203, 1334, 1664, 1734, 2072, 2110, 2182
HpaII CCGG 2 cut(s) 530, 833
HphI GGTGA 9 cut(s) 75, 370, 748, 788, 1636, 2345, 2542, 2621, 2672
Hpy166II GTNNAC 5 cut(s) 649, 670, 1921, 2325, 2510
Hpy8I GTNNAC 5 cut(s) 649, 670, 1921, 2325, 2510
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 4 cut(s) 220, 721, 815, 1210
HpyCH4III ACNGT 8 cut(s) 196, 801, 1298, 1317, 1553, 1904, 2190, 2196
HpyCH4IV ACGT 2 cut(s) 1152, 2321
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 671, 1073, 1409
HpyF3I CTNAG 9 cut(s) 183, 464, 549, 792, 1072, 1212, 2226, 2311, 2540
HpySE526I ACGT 2 cut(s) 1152, 2321
HspAI GCGC 3 cut(s) 395, 397, 411
KpnI GGTACC 1 cut(s) 1878
Ksp22I TGATCA 2 cut(s) 1374, 1837
Kzo9I GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
LmnI GCTCC 3 cut(s) 493, 2146, 2504
Lsp1109I GCAGC 2 cut(s) 1195, 2074
MabI ACCWGGT 1 cut(s) 1972
MaeI CTAG 4 cut(s) 864, 1062, 1290, 1979
MaeII ACGT 2 cut(s) 1152, 2321
MalI GATC 8 cut(s) 302, 1232, 1236, 1376, 1407, 1634, 1839, 2004
MbiI CCGCTC 1 cut(s) 1395
MboI GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
MflI RGATCY 3 cut(s) 300, 1632, 2002
MhlI GDGCHC 1 cut(s) 180
MlyI GAGTC 3 cut(s) 137, 1673, 2066
MmeI TCCRAC 5 cut(s) 62, 275, 722, 1089, 1141
Mph1103I ATGCAT 1 cut(s) 1900
MseI TTAA 5 cut(s) 1088, 1452, 1557, 1860, 2364
MslI CAYNNNNRTG 6 cut(s) 774, 888, 1178, 1325, 1350, 1641
MspA1I CMGCKG 1 cut(s) 1843
MspI CCGG 2 cut(s) 530, 833
MspR9I CCNGG 5 cut(s) 834, 1258, 1363, 1974, 2169
Mva1269I GAATGC 2 cut(s) 751, 1755
MvaI CCWGG 4 cut(s) 1258, 1363, 1974, 2169
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 4 cut(s) 171, 671, 1073, 1409
NciI CCSGG 1 cut(s) 834
NcoI CCATGG 1 cut(s) 1636
NdeI CATATG 1 cut(s) 2206
NdeII GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
NlaIV GGNNCC 4 cut(s) 1876, 2166, 2459, 2500
NmuCI GTSAC 5 cut(s) 190, 1357, 2333, 2612, 2660
NsbI TGCGCA 1 cut(s) 412
NsiI ATGCAT 1 cut(s) 1900
NspI RCATGY 2 cut(s) 1390, 2106
OliI CACNNNNGTG 1 cut(s) 774
PagI TCATGA 1 cut(s) 1320
PaqCI CACCTGC 1 cut(s) 2321
PauI GCGCGC 1 cut(s) 395
PceI AGGCCT 2 cut(s) 1307, 1547
PctI GAATGC 2 cut(s) 751, 1755
PfeI GAWTC 5 cut(s) 203, 1334, 1734, 2110, 2182
PflMI CCANNNNNTGG 1 cut(s) 1432
PkrI GCNGC 3 cut(s) 1210, 2089, 2266
PleI GAGTC 3 cut(s) 137, 1672, 2066
PpsI GAGTC 3 cut(s) 137, 1672, 2066
Ppu21I YACGTR 1 cut(s) 2322
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 4 cut(s) 1256, 1361, 1972, 2167
PspFI CCCAGC 1 cut(s) 2454
PspGI CCWGG 4 cut(s) 1256, 1361, 1972, 2167
PspN4I GGNNCC 4 cut(s) 1876, 2166, 2459, 2500
PspPI GGNCC 2 cut(s) 1417, 2458
PstI CTGCAG 1 cut(s) 2332
PstNI CAGNNNCTG 2 cut(s) 799, 2194
PsuI RGATCY 3 cut(s) 300, 1632, 2002
PteI GCGCGC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 1843
RsaI GTAC 7 cut(s) 434, 671, 1038, 1155, 1876, 2324, 2511
RsaNI GTAC 7 cut(s) 433, 670, 1037, 1154, 1875, 2323, 2510
RseI CAYNNNNRTG 6 cut(s) 774, 888, 1178, 1325, 1350, 1641
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 5 cut(s) 1088, 1452, 1557, 1860, 2364
SatI GCNGC 3 cut(s) 1209, 2088, 2265
Sau3AI GATC 8 cut(s) 300, 1230, 1234, 1374, 1405, 1632, 1837, 2002
Sau96I GGNCC 2 cut(s) 1417, 2458
SchI GAGTC 3 cut(s) 137, 1673, 2066
ScrFI CCNGG 5 cut(s) 834, 1258, 1363, 1974, 2169
SduI GDGCHC 1 cut(s) 180
SexAI ACCWGGT 1 cut(s) 1972
SfcI CTRYAG 2 cut(s) 2061, 2328
SinI GGWCC 2 cut(s) 1417, 2458
SmiMI CAYNNNNRTG 6 cut(s) 774, 888, 1178, 1325, 1350, 1641
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
SnaBI TACGTA 1 cut(s) 2322
SpeI ACTAGT 1 cut(s) 1061
SseBI AGGCCT 2 cut(s) 1307, 1547
SsiI CCGC 3 cut(s) 378, 1395, 2265
SspI AATATT 1 cut(s) 2176
SspMI CTAG 4 cut(s) 864, 1062, 1290, 1979
SstI GAGCTC 1 cut(s) 180
StuI AGGCCT 2 cut(s) 1307, 1547
StyD4I CCNGG 5 cut(s) 832, 1256, 1361, 1972, 2167
StyI CCWWGG 3 cut(s) 1426, 1636, 2127
TaaI ACNGT 8 cut(s) 196, 801, 1298, 1317, 1553, 1904, 2190, 2196
TaiI ACGT 2 cut(s) 1155, 2324
TaqI TCGA 7 cut(s) 224, 656, 711, 1233, 2157, 2257, 2342
TatI WGTACW 1 cut(s) 432
TauI GCSGC 1 cut(s) 2267
TfiI GAWTC 5 cut(s) 203, 1334, 1734, 2110, 2182
Tru1I TTAA 5 cut(s) 1088, 1452, 1557, 1860, 2364
Tru9I TTAA 5 cut(s) 1088, 1452, 1557, 1860, 2364
TscAI CASTG 5 cut(s) 672, 1957, 2294, 2319, 2332
TseFI GTSAC 5 cut(s) 190, 1357, 2333, 2612, 2660
TseI GCWGC 2 cut(s) 1208, 2087
Tsp45I GTSAC 5 cut(s) 190, 1357, 2333, 2612, 2660
TspDTI ATGAA 8 cut(s) 263, 1132, 1309, 1326, 1337, 1726, 2102, 2358
TspGWI ACGGA 1 cut(s) 1657
TspRI CASTG 5 cut(s) 672, 1957, 2294, 2319, 2332
Van91I CCANNNNNTGG 1 cut(s) 1432
VpaK11BI GGWCC 2 cut(s) 1417, 2458
XbaI TCTAGA 1 cut(s) 1978
XceI RCATGY 2 cut(s) 1390, 2106
XcmI CCANNNNNNNNNTGG 1 cut(s) 2639
XmiI GTMKAC 1 cut(s) 1920
XspI CTAG 4 cut(s) 864, 1062, 1290, 1979
Zsp2I ATGCAT 1 cut(s) 1900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.