MD15G1021300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008721
Physical Location & Seq
Forward (+)
1231144 .. 1233813
2670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1021300.v1.1.491

Sequence Viewer

Length: 2670 bp
ATGGAGAATTTTACAATCCCCTGCAAATCAAGCCCTCCAATACCCATAGTACCCTCCAAATTCAGCAACCCATCTGAATTCTTACCACGACAAACCAAACCCACAATCTCCTTTTCCAGAAAAACCCTTCCGAAATTCACAGACTCTCACTTGAATTACCTCCGCAAGAATGGCGAGTTCACGGAGGCCGTCACCGTTCTTGACTCCATTGCCAAAAGTGGGTCGAAGGTAACTTCCACTACTTACATGAATTTACTTCAATCTTGCATTGACACCAATTCTATTCAACTGGGTCGGAAGATCCATGAGCGTATAGATGTAGTTGAGGAATTGAACCCGTTTGTTGAGACGAAGTTGGTGAGCATGTATGCGAAATGCGGGTTTTTGGATGATGCGCGCAAGGTGTTCTACGCAATGCGTGAGAGAAATTTGTACTCTTGGTCGGCCATGATCGGTGCGTGTCTTAGAGATCAGAGGTGGAAAGAGGTGGTGGAGCTTTTTTACTCGATGATGAGAGATGGGGTTTTGCCGGATTACTTTCTGTTCCCTAAGATATTGCAGGCTTGTGGGAATTGTAGCAATTTCGAGGCCACCAAATTGATACACTCCATTGTGGTTCGGTATAATTTGGCTGGTTGTATTCATGTGAACAATTCGATACTGGCGGTGTACGCAAAGTGTGGAAAATTGAAGTGGGCAAGGAGGTTCTTTGACCAGATGGATGAGAAGGATGGGGTGAGTTGGAATGCGGTTATATCTGGTTACTGTCACAAGGGTGAGACCGAAGAGGCTCGGAGACTGTTTGATGCGATGAGCAAAGAAGGGATTGAACCGGGGTTGGTAACTTGGAATACATTGATTGCTAGTCATAACCAGTTGGGTCATTGTGATCTCGCAATGGAACTGATGAGGAGGATGGAGAGCTGTGGCATAACCCCTGATGTATATACTTGGACTTCTTTGATTTCGGGATTTGCTCAAAACGACAGGAAAAATCAGCCTTTGGATTTGTTCAAGATGATGCTTCTATCAGGGGTACAACCAAATGGGATTACAATCACTAGTGCAATCTCAGCATGCACATCTTTAAAATCACTTAACAAAGGGTTGGAAATCTATTCTATTGCTATAAAGATGGGTTTCATTGATGATGTACTGGTCGGAAATGCACTTGTTGATATGTTTTCAAAATGTGGGGAACTGGAAGCTGCTCAGAAGGTCTTTGTTATGATCCCAGAGAAAGACGTCTATACCTGGAACTCAATGATAGGAGGATATTGCCAAGCTAGATACTGTGGTAAGGCCTATGAACTGCTCATGAAGATGCAGGAATCAGATGTGCATCCCAATGCTGTGACCTGGAATGTGATGATCACAGGATACATGCAGAATGGAGATGCGGATCAAGCAATGGACCTCTTCCAAAGGATGGAAAAAGACGGGAAAGTTAAACGAAATACGGCATCATGGAACTCTCTTATCTCTGGCTACCTGCAACTTGGGGAGAAAAACAAGGCACTTGGGGTATTCAGGCAGATGCAGGCCTACTGTGTTAATCCTAATTCTGTTACCATATTGAGTGTCCTGCCCGCTTGTGCAAATCTAGTTGCCACGAAAAAGGTGAGAGAGATCCACGCTGGTGTATTGCGTAGAAATTTGGAGTCTGAAGTCCCTGTTGTAAATTCCCTGATAGACACCTATGCCAAATCGGGAAACATAGCATATCCAAGAATCATATTTGCTAGAATGCCATCCAAAGATATCATCACTTGGAACTCAGCAATTTCTGGTTGTGTCTTACATGGTCTTTCAGACATTGCACTTGACCTTTTTGATCAGCTGAAGAAGTCGGGGTTTAGACCAAATAGAGGTACCTTTACAAGTATCATATATGCATACAGTCTTGCTGGAATGATAGACGAGGGAAGACAAGCTTTTTACAGCATCAGTGAAAACTACCAAATCATACCAGGTCTAGAACATTATTCAGCTATGGTAGATCTTTTCGGGCGTTCTGGAAGACTTCAAGAAGCAATGCAGTTTATTGAAGATATGCCCATAGAACCAGACTCCTCTATTTGGGCTGCCTTATTTACCGCATGTAGGATTCATGGGAATCTTGCCTTGGCAGTTCGTGCAGGGGAGCATTTAATCGACTTGGAACCAGGAAATATTTTGGTTCAACAGTTACTTCTACAGTCATATTCTTTATGTGGTAAGTCTGAGGTTACTTCAAAATTGAAAAAGTTTGGTAGAGATGCTGCAATAAAGAAATTTATTGGGCAGTGCTGGATTGAGGTAAAGAACTCGGTGCATACGTATGTTGCAGGTGATAGGTCGGAACTATGCTCAAACTTCCTAAATTCATGGTTACAAAACATAGAAGAAAAAGCAAAGAGACATGATTTTGGTAATGAGCTTTGTGTTGAGGAAGAAGAGGGGGGTATCAGCTGGGTCCATAGCGAAAAACTGGCACTTGCTTTTGCCCTCATTGGCTCCTCAAGTGTACCTAAGAGTATTAGGATGGTGAAGAATCTGAGAATGTGTGGGGACTGCCACAGGATGGCGAAGTATATATCTATGGCTTTCGGGTGTGAGATATATTTGAGCGACTCAAAGTCCTTTCACCATTTTAGTAATGGCCGTTGTTCATGTGGTGACTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calreticulin DYW_deaminase Jacalin PPR PPR_2 PPR_3

Protein Analysis

890

Amino Acids

99.87

Weight (kDa)

8.1

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 244 - 282 3.7e-10 PPR repeat family
PPR_1 PF12854 244 - 271 9.3e-06 PPR repeat
PPR_3 PF13812 244 - 287 1e-05 Pentatricopeptide repeat domain
PPR PF01535 246 - 276 8.9e-09 PPR repeat
PPR_2 PF13041 278 - 326 4.5e-12 PPR repeat family
PPR_2 PF13041 314 - 360 1.9e-07 PPR repeat family
PPR_2 PF13041 414 - 460 1.4e-12 PPR repeat family
PPR PF01535 417 - 446 2.9e-07 PPR repeat
PPR_long PF17177 434 - 543 1.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 438 - 481 2.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 447 - 478 5e-07 PPR repeat
PPR_2 PF13041 449 - 481 6.4e-09 PPR repeat family
PPR PF01535 452 - 481 7.1e-09 PPR repeat
PPR_2 PF13041 486 - 534 2.6e-08 PPR repeat family
PPR_2 PF13041 586 - 633 4.7e-09 PPR repeat family
DYW_deaminase PF14432 809 - 889 4.4e-27 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2318
AatII GACGTC 1 cut(s) 1248
Acc36I ACCTGC 2 cut(s) 1500, 2318
Acc65I GGTACC 1 cut(s) 1871
AccB1I GGYRCC 1 cut(s) 1871
AccB7I CCANNNNNTGG 2 cut(s) 1429, 2563
AccII CGCG 1 cut(s) 397
AciI CCGC 7 cut(s) 163, 378, 665, 749, 1400, 1590, 2097
AclWI GGATC 4 cut(s) 295, 1225, 1410, 1624
AcoI YGGCCR 2 cut(s) 444, 2641
AcuI CTGAAG 2 cut(s) 1686, 1862
AcyI GRCGYC 1 cut(s) 1245
AfaI GTAC 7 cut(s) 51, 434, 671, 1038, 1155, 1873, 2508
AfiI CCNNNNNNNGG 6 cut(s) 219, 1429, 1868, 2079, 2103, 2563
AhdI GACNNNNNGTC 1 cut(s) 2617
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 4 cut(s) 1253, 1358, 1969, 2164
AjuI GAANNNNNNNTTGG 2 cut(s) 2108, 2140
AleI CACNNNNGTG 2 cut(s) 774, 1638
AluBI AGCT 9 cut(s) 496, 924, 1208, 1286, 1840, 1934, 1991, 2419, 2451
AluI AGCT 9 cut(s) 496, 924, 1208, 1286, 1840, 1934, 1991, 2419, 2451
Alw26I GTCTC 4 cut(s) 341, 773, 790, 2392
AlwI GGATC 4 cut(s) 295, 1225, 1410, 1624
AoxI GGCC 6 cut(s) 186, 444, 588, 1302, 1542, 2641
ApeKI GCWGC 3 cut(s) 1208, 2084, 2261
Asp718I GGTACC 1 cut(s) 1871
AspLEI GCGC 2 cut(s) 397, 399
AspS9I GGNCC 2 cut(s) 1414, 2455
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 9 cut(s) 184, 370, 748, 788, 1633, 2342, 2539, 2618, 2669
AvaII GGWCC 2 cut(s) 1414, 2455
BaeI ACNNNNGTAYC 2 cut(s) 1282, 1315
BanI GGYRCC 1 cut(s) 1871
BbsI GAAGAC 2 cut(s) 1933, 2026
BbvI GCAGC 3 cut(s) 1195, 2071, 2248
BceAI ACGGC 3 cut(s) 173, 1476, 2628
BciT130I CCWGG 4 cut(s) 1255, 1360, 1971, 2166
BciVI GTATCC 1 cut(s) 1373
BclI TGATCA 2 cut(s) 1371, 1834
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 4 cut(s) 341, 773, 790, 2392
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 6 cut(s) 864, 1062, 1287, 1604, 1743, 1976
BfmI CTRYAG 1 cut(s) 2195
BfuAI ACCTGC 2 cut(s) 1500, 2318
BfuI GTATCC 1 cut(s) 1373
BglII AGATCT 1 cut(s) 1999
BisI GCNGC 3 cut(s) 1209, 2085, 2262
BlsI GCNGC 3 cut(s) 1210, 2086, 2263
Bme1390I CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
Bme18I GGWCC 2 cut(s) 1414, 2455
BmeRI GACNNNNNGTC 1 cut(s) 2617
BmgT120I GGNCC 2 cut(s) 1414, 2455
BmiI GGNNCC 4 cut(s) 1873, 2163, 2456, 2497
BmrFI CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
BmrI ACTGGG 1 cut(s) 299
BmuI ACTGGG 1 cut(s) 299
BpiI GAAGAC 2 cut(s) 1933, 2026
BpuEI CTTGAG 1 cut(s) 2485
BpuMI CCSGG 1 cut(s) 834
BsaAI YACGTR 1 cut(s) 2319
BsaBI GATNNNNATC 3 cut(s) 1055, 1341, 1401
BsaHI GRCGYC 1 cut(s) 1245
BsaI GGTCTC 1 cut(s) 773
BsaJI CCNNGG 2 cut(s) 833, 2124
Bsc4I CCNNNNNNNGG 6 cut(s) 219, 1429, 1868, 2079, 2103, 2563
Bse1I ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2475
Bse3DI GCAATG 6 cut(s) 207, 420, 903, 1416, 1815, 2040
Bse8I GATNNNNATC 3 cut(s) 1055, 1341, 1401
BseBI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
BseDI CCNNGG 2 cut(s) 833, 2124
BseGI GGATG 9 cut(s) 394, 727, 736, 921, 1342, 1434, 1751, 2529, 2568
BseJI GATNNNNATC 3 cut(s) 1055, 1341, 1401
BseLI CCNNNNNNNGG 6 cut(s) 219, 1429, 1868, 2079, 2103, 2563
BseMI GCAATG 6 cut(s) 207, 420, 903, 1416, 1815, 2040
BseMII CTCAG 5 cut(s) 1086, 1226, 1791, 2214, 2528
BseNI ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2475
BsePI GCGCGC 1 cut(s) 395
BseRI GAGGAG 3 cut(s) 925, 2062, 2488
BseXI GCAGC 3 cut(s) 1195, 2071, 2248
BseYI CCCAGC 1 cut(s) 2451
BsgI GTGCAG 1 cut(s) 2157
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 6 cut(s) 188, 446, 590, 1304, 1544, 2643
BshNI GGYRCC 1 cut(s) 1871
BsiSI CCGG 2 cut(s) 530, 833
BslFI GGGAC 2 cut(s) 1655, 2564
BslI CCNNNNNNNGG 6 cut(s) 219, 1429, 1868, 2079, 2103, 2563
BsmAI GTCTC 4 cut(s) 341, 773, 790, 2392
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 1655, 2564
BsmI GAATGC 2 cut(s) 751, 1752
BsnI GGCC 6 cut(s) 188, 446, 590, 1304, 1544, 2643
Bso31I GGTCTC 1 cut(s) 773
BspACI CCGC 7 cut(s) 163, 378, 665, 749, 1400, 1590, 2097
BspANI GGCC 6 cut(s) 188, 446, 590, 1304, 1544, 2643
BspCNI CTCAG 5 cut(s) 1085, 1225, 1790, 2215, 2529
BspFNI CGCG 1 cut(s) 397
BspHI TCATGA 1 cut(s) 1317
BspLI GGNNCC 4 cut(s) 1873, 2163, 2456, 2497
BspMI ACCTGC 2 cut(s) 1500, 2318
BspPI GGATC 4 cut(s) 295, 1225, 1410, 1624
BspT107I GGYRCC 1 cut(s) 1871
BspTNI GGTCTC 1 cut(s) 773
BsrDI GCAATG 6 cut(s) 207, 420, 903, 1416, 1815, 2040
BsrI ACTGG 6 cut(s) 294, 666, 874, 1161, 1206, 2475
BssECI CCNNGG 2 cut(s) 833, 2124
BssHII GCGCGC 1 cut(s) 395
BssNI GRCGYC 1 cut(s) 1245
BssT1I CCWWGG 1 cut(s) 2124
Bst2UI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
Bst4CI ACNGT 8 cut(s) 196, 767, 801, 1295, 1550, 1901, 2187, 2199
Bst6I CTCTTC 3 cut(s) 780, 1424, 2430
BstACI GRCGYC 1 cut(s) 1245
BstAPI GCANNNNNTGC 1 cut(s) 2135
BstBAI YACGTR 1 cut(s) 2319
BstC8I GCNNGC 5 cut(s) 397, 561, 1078, 1542, 1590
BstDEI CTNAG 8 cut(s) 464, 549, 1072, 1212, 1777, 2223, 2511, 2537
BstF5I GGATG 9 cut(s) 394, 727, 736, 921, 1342, 1434, 1751, 2529, 2568
BstFNI CGCG 1 cut(s) 397
BstHHI GCGC 2 cut(s) 397, 399
BstMAI GTCTC 4 cut(s) 341, 773, 790, 2392
BstMWI GCNNNNNNNGC 6 cut(s) 30, 171, 671, 1073, 1406, 2135
BstNI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
BstNSI RCATGY 4 cut(s) 367, 1080, 1387, 2103
BstSCI CCNGG 5 cut(s) 832, 1253, 1358, 1969, 2164
BstSFI CTRYAG 1 cut(s) 2195
BstSNI TACGTA 1 cut(s) 2319
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 1195, 2071, 2248
BstV2I GAAGAC 2 cut(s) 1933, 2026
BstX2I RGATCY 3 cut(s) 300, 1629, 1999
BstYI RGATCY 3 cut(s) 300, 1629, 1999
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 6 cut(s) 188, 446, 590, 1304, 1544, 2643
BtgZI GCGATG 1 cut(s) 824
BtsCI GGATG 9 cut(s) 394, 727, 736, 921, 1342, 1434, 1751, 2529, 2568
BtsI GCAGTG 1 cut(s) 2291
BtsIMutI CAGTG 2 cut(s) 1954, 2291
BveI ACCTGC 2 cut(s) 1500, 2318
Cac8I GCNNGC 5 cut(s) 397, 561, 1078, 1542, 1590
CciI TCATGA 1 cut(s) 1317
CfoI GCGC 2 cut(s) 397, 399
Cfr13I GGNCC 2 cut(s) 1414, 2455
CsiI ACCWGGT 1 cut(s) 1969
Csp6I GTAC 7 cut(s) 50, 433, 670, 1037, 1154, 1872, 2507
CviQI GTAC 7 cut(s) 50, 433, 670, 1037, 1154, 1872, 2507
DdeI CTNAG 8 cut(s) 464, 549, 1072, 1212, 1777, 2223, 2511, 2537
DraI TTTAAA 1 cut(s) 1089
DriI GACNNNNNGTC 1 cut(s) 2617
EaeI YGGCCR 2 cut(s) 444, 2641
Eam1104I CTCTTC 3 cut(s) 780, 1424, 2430
Eam1105I GACNNNNNGTC 1 cut(s) 2617
EarI CTCTTC 3 cut(s) 780, 1424, 2430
Eco105I TACGTA 1 cut(s) 2319
Eco130I CCWWGG 1 cut(s) 2124
Eco147I AGGCCT 2 cut(s) 1304, 1544
Eco31I GGTCTC 1 cut(s) 773
Eco32I GATATC 1 cut(s) 1762
Eco47I GGWCC 2 cut(s) 1414, 2455
Eco57I CTGAAG 2 cut(s) 1686, 1862
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 1253, 1358, 1969, 2164
EcoRV GATATC 1 cut(s) 1762
EcoT14I CCWWGG 1 cut(s) 2124
EcoT22I ATGCAT 1 cut(s) 1897
ErhI CCWWGG 1 cut(s) 2124
Esp3I CGTCTC 1 cut(s) 341
FalI AAGNNNNNCTT 2 cut(s) 1918, 1950
FaqI GGGAC 2 cut(s) 1655, 2564
FauI CCCGC 2 cut(s) 371, 1597
FbaI TGATCA 2 cut(s) 1371, 1834
Fnu4HI GCNGC 3 cut(s) 1209, 2085, 2262
FokI GGATG 9 cut(s) 401, 734, 743, 928, 1329, 1441, 1738, 2536, 2575
Fsp4HI GCNGC 3 cut(s) 1209, 2085, 2262
FspBI CTAG 6 cut(s) 864, 1062, 1287, 1604, 1743, 1976
GlaI GCGC 2 cut(s) 396, 398
GluI GCNGC 3 cut(s) 1209, 2085, 2262
GsaI CCCAGC 1 cut(s) 2455
HaeIII GGCC 6 cut(s) 188, 446, 590, 1304, 1544, 2643
HapII CCGG 2 cut(s) 530, 833
HhaI GCGC 2 cut(s) 397, 399
Hin1I GRCGYC 1 cut(s) 1245
Hin6I GCGC 2 cut(s) 395, 397
HinP1I GCGC 2 cut(s) 395, 397
HindIII AAGCTT 1 cut(s) 1932
HpaII CCGG 2 cut(s) 530, 833
HphI GGTGA 9 cut(s) 184, 370, 748, 788, 1633, 2342, 2539, 2618, 2669
Hpy166II GTNNAC 4 cut(s) 180, 649, 670, 2507
Hpy188III TCNNGA 9 cut(s) 117, 200, 969, 1015, 1318, 1710, 1976, 2016, 2027
Hpy8I GTNNAC 4 cut(s) 180, 649, 670, 2507
HpyAV CCTTC 5 cut(s) 137, 220, 721, 815, 1210
HpyCH4III ACNGT 8 cut(s) 196, 767, 801, 1295, 1550, 1901, 2187, 2199
HpyCH4IV ACGT 2 cut(s) 1245, 2318
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 171, 671, 1073, 1406, 2135
HpyF3I CTNAG 8 cut(s) 464, 549, 1072, 1212, 1777, 2223, 2511, 2537
HpySE526I ACGT 2 cut(s) 1245, 2318
Hsp92I GRCGYC 1 cut(s) 1245
HspAI GCGC 2 cut(s) 395, 397
KpnI GGTACC 1 cut(s) 1875
Ksp22I TGATCA 2 cut(s) 1371, 1834
LmnI GCTCC 3 cut(s) 493, 2143, 2501
Lsp1109I GCAGC 3 cut(s) 1195, 2071, 2248
MabI ACCWGGT 1 cut(s) 1969
MaeI CTAG 6 cut(s) 864, 1062, 1287, 1604, 1743, 1976
MaeII ACGT 2 cut(s) 1245, 2318
MflI RGATCY 3 cut(s) 300, 1629, 1999
MlyI GAGTC 5 cut(s) 137, 197, 1670, 2063, 2606
MmeI TCCRAC 5 cut(s) 275, 722, 1089, 1141, 2319
Mph1103I ATGCAT 1 cut(s) 1897
MseI TTAA 5 cut(s) 1088, 1098, 1449, 1554, 2150
MslI CAYNNNNRTG 5 cut(s) 774, 1322, 1347, 1638, 2319
MspA1I CMGCKG 2 cut(s) 1840, 2451
MspI CCGG 2 cut(s) 530, 833
MspR9I CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
Mva1269I GAATGC 2 cut(s) 751, 1752
MvaI CCWGG 4 cut(s) 1255, 1360, 1971, 2166
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 6 cut(s) 30, 171, 671, 1073, 1406, 2135
NciI CCSGG 1 cut(s) 834
NlaIV GGNNCC 4 cut(s) 1873, 2163, 2456, 2497
NmuCI GTSAC 4 cut(s) 190, 767, 1354, 2657
NsiI ATGCAT 1 cut(s) 1897
NspI RCATGY 4 cut(s) 367, 1080, 1387, 2103
OliI CACNNNNGTG 2 cut(s) 774, 1638
PaeI GCATGC 1 cut(s) 1080
PagI TCATGA 1 cut(s) 1317
PaqCI CACCTGC 1 cut(s) 2318
PauI GCGCGC 1 cut(s) 395
PceI AGGCCT 2 cut(s) 1304, 1544
PcsI WCGNNNNNNNCGW 1 cut(s) 2315
PctI GAATGC 2 cut(s) 751, 1752
PfeI GAWTC 5 cut(s) 1331, 1731, 2107, 2116, 2533
PflMI CCANNNNNTGG 2 cut(s) 1429, 2563
PkrI GCNGC 3 cut(s) 1210, 2086, 2263
PleI GAGTC 5 cut(s) 137, 197, 1669, 2063, 2606
PpsI GAGTC 5 cut(s) 137, 197, 1669, 2063, 2606
Ppu21I YACGTR 1 cut(s) 2319
Psp6I CCWGG 4 cut(s) 1253, 1358, 1969, 2164
PspFI CCCAGC 1 cut(s) 2451
PspGI CCWGG 4 cut(s) 1253, 1358, 1969, 2164
PspN4I GGNNCC 4 cut(s) 1873, 2163, 2456, 2497
PspPI GGNCC 2 cut(s) 1414, 2455
PsuI RGATCY 3 cut(s) 300, 1629, 1999
PteI GCGCGC 1 cut(s) 395
PvuII CAGCTG 2 cut(s) 1840, 2451
RsaI GTAC 7 cut(s) 51, 434, 671, 1038, 1155, 1873, 2508
RsaNI GTAC 7 cut(s) 50, 433, 670, 1037, 1154, 1872, 2507
RseI CAYNNNNRTG 5 cut(s) 774, 1322, 1347, 1638, 2319
SaqAI TTAA 5 cut(s) 1088, 1098, 1449, 1554, 2150
SatI GCNGC 3 cut(s) 1209, 2085, 2262
Sau96I GGNCC 2 cut(s) 1414, 2455
SchI GAGTC 5 cut(s) 137, 197, 1670, 2063, 2606
ScrFI CCNGG 5 cut(s) 834, 1255, 1360, 1971, 2166
SexAI ACCWGGT 1 cut(s) 1969
SfcI CTRYAG 1 cut(s) 2195
SinI GGWCC 2 cut(s) 1414, 2455
SmiMI CAYNNNNRTG 5 cut(s) 774, 1322, 1347, 1638, 2319
SmlI CTYRAG 1 cut(s) 2500
SmoI CTYRAG 1 cut(s) 2500
SnaBI TACGTA 1 cut(s) 2319
SpeI ACTAGT 1 cut(s) 1061
SphI GCATGC 1 cut(s) 1080
SseBI AGGCCT 2 cut(s) 1304, 1544
SsiI CCGC 7 cut(s) 163, 378, 665, 749, 1400, 1590, 2097
SspI AATATT 1 cut(s) 2173
SspMI CTAG 6 cut(s) 864, 1062, 1287, 1604, 1743, 1976
StuI AGGCCT 2 cut(s) 1304, 1544
StyD4I CCNGG 5 cut(s) 832, 1253, 1358, 1969, 2164
StyI CCWWGG 1 cut(s) 2124
TaaI ACNGT 8 cut(s) 196, 767, 801, 1295, 1550, 1901, 2187, 2199
TaiI ACGT 2 cut(s) 1248, 2321
TaqI TCGA 5 cut(s) 224, 506, 585, 656, 2154
TaqII GACCGA 1 cut(s) 797
TatI WGTACW 2 cut(s) 432, 1153
TfiI GAWTC 5 cut(s) 1331, 1731, 2107, 2116, 2533
Tru1I TTAA 5 cut(s) 1088, 1098, 1449, 1554, 2150
Tru9I TTAA 5 cut(s) 1088, 1098, 1449, 1554, 2150
TscAI CASTG 2 cut(s) 1954, 2291
TseFI GTSAC 4 cut(s) 190, 767, 1354, 2657
TseI GCWGC 3 cut(s) 1208, 2084, 2261
Tsp45I GTSAC 4 cut(s) 190, 767, 1354, 2657
TspDTI ATGAA 8 cut(s) 263, 632, 1132, 1323, 1334, 2099, 2355, 2640
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 2 cut(s) 1954, 2291
Van91I CCANNNNNTGG 2 cut(s) 1429, 2563
VpaK11BI GGWCC 2 cut(s) 1414, 2455
XbaI TCTAGA 1 cut(s) 1975
XceI RCATGY 4 cut(s) 367, 1080, 1387, 2103
XcmI CCANNNNNNNNNTGG 1 cut(s) 2636
XspI CTAG 6 cut(s) 864, 1062, 1287, 1604, 1743, 1976
ZraI GACGTC 1 cut(s) 1246
Zsp2I ATGCAT 1 cut(s) 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.