MD08G1030100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012084
Physical Location & Seq
Reverse (-)
2172547 .. 2174336
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1030100.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGGCAGACAAAAAGCATCCACAATCATTTCACTTTCGGCTCCCTTGGCGTACTCGTGGTTCTCAAGTAGAACCCGACCCACGTCCAGTGGCTAAAACCCAAAACCCTGCCCAGACCACCACCTCAATCCCTGTTGAGCGACCACCATTTCGGCCTGCAGGGAAAGCCCCTGTGAAAGCCTCTCCTTCCCAAGCTCAGGCACCGTCAAGGAATGAACCTCCACCACCATCACCATCTCGTTCTCCCACCGAGTCCCAAAATTCTACTAGTGGAACAACTCAAACTCGGGTTGAAGTTGAATCCTCTCCATCCTCACCATCCTCACCATCTCGCCCTGCCACCGAGTCCCAAAATTCTTCTAGTCGAACAACTCAAACTCGGGTTGAAGTTGAATCCTCTCCACCCTCACCGTCTCATGCTGCCACTGAGTCCCGAAAAGCTTCTAGTGCAACAGCTCAGACTCGGGTTGAATCCTTTCGATCCTCACCATCTCCCATAGCTTCTCAACCCCAAGCAGCATCACAAGAATCCCCTAAAGCGCATTCGCAGCCCCACACTCAAGAGAACTCTCAGCTTCCTTCCTTCTCTGCTGAATCTCCATCACCTGCCACTCAACCAGAACCAAAGGAACCATATGCAGCAATCAGCACATCCGAAACTATTTTCAAAGCTCAGAATAATACTCCAACCACCGTTCAAACTCATGCAGCTTCCGCAGAAGCTGTGGAAACCGTTGCTTCACCCAAGAAGGTAGATCCTGATACCATTGGCGGAGATCAAAAGCACCATCCCGAATCAGAGACAGAGTCGAAAGAGCATAAAGAAAGGGAGAAAGTACTGCTAGTGATAAAAGAAGAAAAGGCAAACGATCCAGACTATAAAGAACTGATGCAGGAAACAGCCACTCAACTTCATGCTGCAGTAACAGGCTTCGGAAAGCACACCAAAGACCTACTTGAAGTTGCATCGCAAGCAGAGCAGCGGCACAGTAAGGAAGAAGCTTTTGACAGAAAGGAGACATTAGCCAGCTCCAGCTCCAGTTCGAAACTGACCAAAGCTCCAGAAGACACAAGCACCCCAAGGATATCTATTCAGAAGATGGTTTCATCAACCGTGAAGCAAGCTCCCCTGCATAGAGAAATCAGGGAAAATGCTTTGAAACTTAATAAGCTGGCCATTGAGGACCAAATGCATGAAAAACCAGTTAGCATTGTAACTGTAGCTGGTGAAAACAGAGGAGCATCAATGCACATTAGCTCAGAACCGGAAAAAGGAGGAGAATTCATGCCCATACACCGCAGTTATAAGACCAATCCAGATGACAGCCTGGAGGCTACTACTGATGGAGAGGTAAGCAGCGAGGGGGAAAAATTCGATCAAACAAGTAAAGAAGATCGACATATGATAGCATATATCAACAACAATGTACAGAGCATCAATAATGCTATCGTTCTCAATACTTCCGTGGCCGAAAGGAATCCAGGTGTTCAGACGGTTTTTTCTCATAGCCCAGCAGAATCAATCAAGCCAAATGGTAAATCAGATCCCCTCAAAACACACAAGGCTCAAATAACAAAAACACCGTCTGAGAAGCTTACTCACGAATCCACTGTGAGACGGAGGTGCCTTCGAGGGCTCTTCATGGAACCAAGTGATTCAGACCCGGATAATCCTGAAAAGCCTCGGCGTCATGGCTGCCGCTATAGTTGCGGAATGAATAGAGAAGATACCAATACTGGGATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

583

Amino Acids

63.53

Weight (kDa)

6.74

Isoelectric Point (pI)

67.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75260
fragaria_vesca FvH4_2g39641
malus_domestica MD08G1030100.v1.1 MD15G1025500.v1.1
prunus_persica Prupe.1G377800_v2.0.a1
pyrus_communis pycom08g02390 pycom15g02190
rosa_chinensis RchiOBHm_Chr6g0301251
rosa_laevigata RLG00000011267
rosa_multiflora Rmu_sc0013354.1_g000012
rosa_roxburghii Rroxscaffold_171G00441310 Rroxscaffold_7G00167040
rosa_rugosa Rorug06G0302700
rosa_samantha Rh6AG416100 Rh6BG422800 Rh6BG422900 Rh6CG430500 Rh6DG416100
rosa_wichuraiana Rw6G036030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1305
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 613
AccB1I GGYRCC 2 cut(s) 199, 1623
AciI CCGC 6 cut(s) 714, 771, 982, 1297, 1699, 1710
AclWI GGATC 4 cut(s) 474, 749, 863, 1538
AcoI YGGCCR 2 cut(s) 1173, 1467
AcsI RAATTY 4 cut(s) 259, 352, 1280, 1370
AcyI GRCGYC 1 cut(s) 1687
AfaI GTAC 3 cut(s) 52, 837, 1428
AfiI CCNNNNNNNGG 3 cut(s) 196, 1271, 1737
AgsI TTSAA 9 cut(s) 293, 299, 386, 392, 470, 667, 698, 959, 1159
AhlI ACTAGT 1 cut(s) 266
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 2 cut(s) 1326, 1480
Alw26I GTCTC 4 cut(s) 417, 794, 1010, 1609
AlwI GGATC 4 cut(s) 474, 749, 863, 1538
AlwNI CAGNNNCTG 1 cut(s) 722
Ama87I CYCGRG 3 cut(s) 285, 378, 462
AoxI GGCC 3 cut(s) 152, 1173, 1467
ApeKI GCWGC 9 cut(s) 419, 515, 547, 638, 707, 917, 979, 1356, 1695
ApoI RAATTY 4 cut(s) 259, 352, 1280, 1370
ArsI GACNNNNNNTTYG 4 cut(s) 132, 164, 1568, 1600
Asp700I GAANNNNTTC 2 cut(s) 439, 474
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 1183
AsuC2I CCSGG 1 cut(s) 1664
AsuHPI GGTGA 8 cut(s) 222, 306, 315, 399, 477, 594, 732, 1238
AsuII TTCGAA 1 cut(s) 1043
AvaI CYCGRG 3 cut(s) 285, 378, 462
AvaII GGWCC 1 cut(s) 1183
BalI TGGCCA 1 cut(s) 1175
BanI GGYRCC 2 cut(s) 199, 1623
BanII GRGCYC 1 cut(s) 1638
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 1071
BbvI GCAGC 9 cut(s) 406, 527, 559, 650, 719, 904, 991, 1368, 1682
BciT130I CCWGG 2 cut(s) 1328, 1482
BcnI CCSGG 1 cut(s) 1664
BcoDI GTCTC 4 cut(s) 417, 794, 1010, 1609
BcuI ACTAGT 1 cut(s) 266
BfaI CTAG 4 cut(s) 267, 360, 444, 842
BfmI CTRYAG 4 cut(s) 156, 918, 1218, 1702
BfuAI ACCTGC 1 cut(s) 613
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 3 cut(s) 1328, 1482, 1664
Bme18I GGWCC 1 cut(s) 1183
BmeT110I CYCGRG 3 cut(s) 285, 378, 462
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 1183
BmiI GGNNCC 5 cut(s) 41, 201, 630, 1625, 1647
BmrFI CCNGG 3 cut(s) 1328, 1482, 1664
BmrI ACTGGG 1 cut(s) 1746
BmsI GCATC 6 cut(s) 25, 527, 879, 974, 1250, 1443
BmuI ACTGGG 1 cut(s) 1746
BoxI GACNNNNGTC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 1071
BpmI CTGGAG 4 cut(s) 1015, 1021, 1044, 1349
Bpu10I CCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 1043
BpuEI CTTGAG 2 cut(s) 48, 543
BpuMI CCSGG 1 cut(s) 1664
BsaHI GRCGYC 1 cut(s) 1687
BsaJI CCNNGG 4 cut(s) 44, 1079, 1464, 1682
BsaWI WCCGGW 1 cut(s) 1264
BsaXI ACNNNNNCTCC 2 cut(s) 1042, 1072
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 1271, 1737
Bse1I ACTGG 4 cut(s) 86, 1038, 1202, 1741
BseBI CCWGG 2 cut(s) 1328, 1482
BseDI CCNNGG 4 cut(s) 44, 1079, 1464, 1682
BseGI GGATG 5 cut(s) 16, 308, 317, 650, 787
BseLI CCNNNNNNNGG 3 cut(s) 196, 1271, 1737
BseMII CTCAG 7 cut(s) 209, 417, 470, 584, 686, 1272, 1578
BseNI ACTGG 4 cut(s) 86, 1038, 1202, 1741
BseRI GAGGAG 2 cut(s) 1251, 1290
BseXI GCAGC 9 cut(s) 406, 527, 559, 650, 719, 904, 991, 1368, 1682
BseYI CCCAGC 1 cut(s) 1510
BshFI GGCC 3 cut(s) 154, 1175, 1469
BshNI GGYRCC 2 cut(s) 199, 1623
BsiHKCI CYCGRG 3 cut(s) 285, 378, 462
BsiSI CCGG 2 cut(s) 1265, 1664
BslFI GGGAC 3 cut(s) 238, 331, 415
BslI CCNNNNNNNGG 3 cut(s) 196, 1271, 1737
BsmAI GTCTC 4 cut(s) 417, 794, 1010, 1609
BsmBI CGTCTC 2 cut(s) 417, 1609
BsmFI GGGAC 3 cut(s) 238, 331, 415
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 3 cut(s) 154, 1175, 1469
BsoBI CYCGRG 3 cut(s) 285, 378, 462
Bsp119I TTCGAA 1 cut(s) 1043
Bsp1286I GDGCHC 1 cut(s) 1638
Bsp1407I TGTACA 1 cut(s) 1426
Bsp143I GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
BspACI CCGC 6 cut(s) 714, 771, 982, 1297, 1699, 1710
BspANI GGCC 3 cut(s) 154, 1175, 1469
BspCNI CTCAG 7 cut(s) 208, 418, 469, 583, 685, 1271, 1579
BspLI GGNNCC 5 cut(s) 41, 201, 630, 1625, 1647
BspMAI CTGCAG 2 cut(s) 160, 922
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 4 cut(s) 474, 749, 863, 1538
BspQI GCTCTTC 1 cut(s) 1643
BspT104I TTCGAA 1 cut(s) 1043
BspT107I GGYRCC 2 cut(s) 199, 1623
BsrGI TGTACA 1 cut(s) 1426
BsrI ACTGG 4 cut(s) 86, 1038, 1202, 1741
BssECI CCNNGG 4 cut(s) 44, 1079, 1464, 1682
BssMI GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
BssNI GRCGYC 1 cut(s) 1687
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 44, 1079
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 1328, 1482
Bst6I CTCTTC 1 cut(s) 1643
BstACI GRCGYC 1 cut(s) 1687
BstAUI TGTACA 1 cut(s) 1426
BstBI TTCGAA 1 cut(s) 1043
BstC8I GCNNGC 5 cut(s) 156, 972, 1027, 1122, 1173
BstDEI CTNAG 7 cut(s) 195, 426, 456, 570, 672, 1258, 1587
BstDSI CCRYGG 1 cut(s) 1464
BstF5I GGATG 5 cut(s) 16, 308, 317, 650, 787
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 7 cut(s) 482, 757, 778, 871, 1378, 1396, 1546
BstMAI GTCTC 4 cut(s) 417, 794, 1010, 1609
BstMBI GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
BstMWI GCNNNNNNNGC 8 cut(s) 46, 164, 446, 547, 713, 971, 976, 1707
BstNI CCWGG 2 cut(s) 1328, 1482
BstPAI GACNNNNGTC 1 cut(s) 81
BstSCI CCNGG 3 cut(s) 1326, 1480, 1662
BstSFI CTRYAG 4 cut(s) 156, 918, 1218, 1702
BstV1I GCAGC 9 cut(s) 406, 527, 559, 650, 719, 904, 991, 1368, 1682
BstV2I GAAGAC 1 cut(s) 1071
BstX2I RGATCY 2 cut(s) 754, 1543
BstYI RGATCY 2 cut(s) 754, 1543
BsuRI GGCC 3 cut(s) 154, 1175, 1469
BtgI CCRYGG 1 cut(s) 1464
BtgZI GCGATG 1 cut(s) 951
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 5 cut(s) 16, 308, 317, 650, 787
BtsIMutI CAGTG 3 cut(s) 93, 423, 1608
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 5 cut(s) 156, 972, 1027, 1122, 1173
CaiI CAGNNNCTG 1 cut(s) 722
CfoI GCGC 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 1183
CseI GACGC 1 cut(s) 1676
Csp6I GTAC 3 cut(s) 51, 836, 1427
CviAII CATG 7 cut(s) 416, 704, 914, 1193, 1285, 1642, 1691
CviQI GTAC 3 cut(s) 51, 836, 1427
DdeI CTNAG 7 cut(s) 195, 426, 456, 570, 672, 1258, 1587
DpnI GATC 7 cut(s) 481, 756, 777, 870, 1377, 1395, 1545
DpnII GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
EaeI YGGCCR 2 cut(s) 1173, 1467
Eam1104I CTCTTC 1 cut(s) 1643
EarI CTCTTC 1 cut(s) 1643
EciI GGCGGA 1 cut(s) 786
Eco130I CCWWGG 2 cut(s) 44, 1079
Eco24I GRGCYC 1 cut(s) 1638
Eco32I GATATC 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 1183
Eco88I CYCGRG 3 cut(s) 285, 378, 462
EcoRI GAATTC 1 cut(s) 1280
EcoRII CCWGG 2 cut(s) 1326, 1480
EcoRV GATATC 1 cut(s) 1086
EcoT14I CCWWGG 2 cut(s) 44, 1079
EcoT22I ATGCAT 1 cut(s) 1194
EcoT38I GRGCYC 1 cut(s) 1638
ErhI CCWWGG 2 cut(s) 44, 1079
Esp3I CGTCTC 2 cut(s) 417, 1609
FaeI CATG 7 cut(s) 419, 707, 917, 1196, 1288, 1645, 1694
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FaqI GGGAC 3 cut(s) 238, 331, 415
FatI CATG 7 cut(s) 415, 703, 913, 1192, 1284, 1641, 1690
FauNDI CATATG 2 cut(s) 634, 1401
FokI GGATG 5 cut(s) 3, 295, 304, 637, 774
FriOI GRGCYC 1 cut(s) 1638
FspBI CTAG 4 cut(s) 267, 360, 444, 842
GlaI GCGC 1 cut(s) 540
GsaI CCCAGC 1 cut(s) 1514
GsuI CTGGAG 4 cut(s) 1015, 1021, 1044, 1349
HaeIII GGCC 3 cut(s) 154, 1175, 1469
HapII CCGG 2 cut(s) 1265, 1664
HgaI GACGC 1 cut(s) 1676
HhaI GCGC 1 cut(s) 541
Hin1I GRCGYC 1 cut(s) 1687
Hin1II CATG 7 cut(s) 419, 707, 917, 1196, 1288, 1645, 1694
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HindIII AAGCTT 3 cut(s) 438, 999, 1592
HpaII CCGG 2 cut(s) 1265, 1664
HphI GGTGA 8 cut(s) 222, 306, 315, 399, 477, 594, 732, 1238
Hpy188III TCNNGA 9 cut(s) 432, 560, 758, 791, 872, 1061, 1316, 1601, 1673
HpyAV CCTTC 5 cut(s) 195, 588, 592, 742, 1637
HpyCH4IV ACGT 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 164, 446, 547, 713, 971, 976, 1707
HpyF3I CTNAG 7 cut(s) 195, 426, 456, 570, 672, 1258, 1587
HpySE526I ACGT 1 cut(s) 82
Hsp92I GRCGYC 1 cut(s) 1687
Hsp92II CATG 7 cut(s) 419, 707, 917, 1196, 1288, 1645, 1694
HspAI GCGC 1 cut(s) 539
Kzo9I GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
LguI GCTCTTC 1 cut(s) 1643
LmnI GCTCC 6 cut(s) 45, 1034, 1040, 1063, 1129, 1238
Lsp1109I GCAGC 9 cut(s) 406, 527, 559, 650, 719, 904, 991, 1368, 1682
LweI GCATC 6 cut(s) 25, 527, 879, 974, 1250, 1443
MaeI CTAG 4 cut(s) 267, 360, 444, 842
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 2 cut(s) 922, 1213
MalI GATC 7 cut(s) 481, 756, 777, 870, 1377, 1395, 1545
MboI GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
MboII GAAGA 8 cut(s) 348, 866, 1007, 1076, 1108, 1403, 1630, 1736
MflI RGATCY 2 cut(s) 754, 1543
MhlI GDGCHC 1 cut(s) 1638
MlsI TGGCCA 1 cut(s) 1175
MluCI AATT 4 cut(s) 259, 352, 1280, 1370
MluNI TGGCCA 1 cut(s) 1175
MlyI GAGTC 5 cut(s) 260, 353, 437, 454, 815
MmeI TCCRAC 1 cut(s) 710
Mox20I TGGCCA 1 cut(s) 1175
Mph1103I ATGCAT 1 cut(s) 1194
MroXI GAANNNNTTC 2 cut(s) 439, 474
MscI TGGCCA 1 cut(s) 1175
MseI TTAA 1 cut(s) 1164
Msp20I TGGCCA 1 cut(s) 1175
MspA1I CMGCKG 1 cut(s) 982
MspI CCGG 2 cut(s) 1265, 1664
MspR9I CCNGG 3 cut(s) 1328, 1482, 1664
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 1328, 1482
MwoI GCNNNNNNNGC 8 cut(s) 46, 164, 446, 547, 713, 971, 976, 1707
NciI CCSGG 1 cut(s) 1664
NdeI CATATG 2 cut(s) 634, 1401
NdeII GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
NlaIII CATG 7 cut(s) 419, 707, 917, 1196, 1288, 1645, 1694
NlaIV GGNNCC 5 cut(s) 41, 201, 630, 1625, 1647
NmeAIII GCCGAG 1 cut(s) 1663
NsiI ATGCAT 1 cut(s) 1194
NspV TTCGAA 1 cut(s) 1043
PaqCI CACCTGC 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 1643
PctI GAATGC 1 cut(s) 541
PdmI GAANNNNTTC 2 cut(s) 439, 474
PflFI GACNNNGTC 1 cut(s) 805
PleI GAGTC 5 cut(s) 259, 352, 436, 454, 814
PpsI GAGTC 5 cut(s) 259, 352, 436, 454, 814
PshAI GACNNNNGTC 1 cut(s) 81
PsiI TTATAA 1 cut(s) 1305
Psp6I CCWGG 2 cut(s) 1326, 1480
PspFI CCCAGC 1 cut(s) 1510
PspGI CCWGG 2 cut(s) 1326, 1480
PspN4I GGNNCC 5 cut(s) 41, 201, 630, 1625, 1647
PspPI GGNCC 1 cut(s) 1183
PsrI GAACNNNNNNTAC 2 cut(s) 43, 75
PstI CTGCAG 2 cut(s) 160, 922
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 2 cut(s) 754, 1543
PsyI GACNNNGTC 1 cut(s) 805
RsaI GTAC 3 cut(s) 52, 837, 1428
RsaNI GTAC 3 cut(s) 51, 836, 1427
SapI GCTCTTC 1 cut(s) 1643
SaqAI TTAA 1 cut(s) 1164
Sau3AI GATC 7 cut(s) 479, 754, 775, 868, 1375, 1393, 1543
Sau96I GGNCC 1 cut(s) 1183
SbfI CCTGCAGG 1 cut(s) 160
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 5 cut(s) 260, 353, 437, 454, 815
ScrFI CCNGG 3 cut(s) 1328, 1482, 1664
SdaI CCTGCAGG 1 cut(s) 160
SduI GDGCHC 1 cut(s) 1638
SfaNI GCATC 6 cut(s) 25, 527, 879, 974, 1250, 1443
SfcI CTRYAG 4 cut(s) 156, 918, 1218, 1702
SfuI TTCGAA 1 cut(s) 1043
SinI GGWCC 1 cut(s) 1183
SmlI CTYRAG 2 cut(s) 63, 558
SmoI CTYRAG 2 cut(s) 63, 558
SpeI ACTAGT 1 cut(s) 266
Sse8387I CCTGCAGG 1 cut(s) 160
Sse9I AATT 4 cut(s) 259, 352, 1280, 1370
SsiI CCGC 6 cut(s) 714, 771, 982, 1297, 1699, 1710
SspMI CTAG 4 cut(s) 267, 360, 444, 842
StyD4I CCNGG 3 cut(s) 1326, 1480, 1662
StyI CCWWGG 2 cut(s) 44, 1079
TaiI ACGT 1 cut(s) 85
TaqI TCGA 7 cut(s) 364, 478, 809, 1043, 1374, 1396, 1630
TasI AATT 4 cut(s) 259, 352, 1280, 1370
TatI WGTACW 2 cut(s) 835, 1426
TauI GCSGC 2 cut(s) 985, 1701
Tru1I TTAA 1 cut(s) 1164
Tru9I TTAA 1 cut(s) 1164
TscAI CASTG 3 cut(s) 93, 430, 1615
TseI GCWGC 9 cut(s) 419, 515, 547, 638, 707, 917, 979, 1356, 1695
TspDTI ATGAA 7 cut(s) 228, 902, 1095, 1209, 1273, 1630, 1730
TspGWI ACGGA 2 cut(s) 1453, 1633
TspRI CASTG 3 cut(s) 93, 430, 1615
Tth111I GACNNNGTC 1 cut(s) 805
VpaK11BI GGWCC 1 cut(s) 1183
XapI RAATTY 4 cut(s) 259, 352, 1280, 1370
XmnI GAANNNNTTC 2 cut(s) 439, 474
XspI CTAG 4 cut(s) 267, 360, 444, 842
ZrmI AGTACT 1 cut(s) 837
Zsp2I ATGCAT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.