MD08G1056100.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012899
Physical Location & Seq
Reverse (-)
4346367 .. 4347315
949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1056100.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCTGGCTTTTCATCCTCAAAAGCTCTGTCATACTCCTCTCTTCTCGTCGTCTTCCTACTCTTCAGCTTCTCAGAAGCCAGAGAGATCACCGTCGGTGGCAAGAATGGCTCATGGGCAGTCGGCTCTGAATCCCAATCCCTCAACAAATGGGCCGAAAGCACCCGCTTTCGCATCGGCGACACTCTTGTGTGGAAGTACGACAGCGCCAAAGACTCAGTCTTGCGAGTGACGAAAGAAGACTACGGAAACTGCAATGCGTCAAACCCAATTGAGCAGCTCAAGGACGGCGAAACAAAGCTCCACCTTGACCAGCCAGGGCCTTACTACTTCATCAGCGGAACCAAGGGACACTGCGAGAAGGGGCAGAAACTGGTGTTGGTGGTTATGACTCCAAGGAAGCACCTCGGTATTTCTCCCGCTCCTTCTCCGGCAGGGGAGGTCGACGGTCCTGCTATTGCTCCGACAAGCGGCGCTACAAGCTTGAAGGGTGGTGTTCTGACTGTGGTGGCATTGGGAGTGTTTTCTCTGTGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

180

Amino Acids

19.15

Weight (kDa)

8.5

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 39 - 123 2.8e-27 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012899)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25060 AT4G31840 AT5G25090
fragaria_vesca FvH4_2g41150
malus_domestica MD08G1056100.v1.1 MD15G1040900.v1.1
prunus_persica Prupe.1G394200_v2.0.a1
pyrus_communis pycom08g04610 pycom15g03810
rosa_chinensis RchiOBHm_Chr6g0299381
rosa_laevigata RLG00000011437
rosa_roxburghii Rroxscaffold_7G00168740
rosa_rugosa Rorug06G0287700
rosa_samantha Rh6AG399300 Rh6BG407800 Rh6CG413600 Rh6DG400500
rosa_wichuraiana Rw6G034960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 422
AccI GTMKAC 1 cut(s) 444
AciI CCGC 4 cut(s) 166, 339, 420, 471
AcuI CTGAAG 1 cut(s) 49
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 1 cut(s) 487
AjnI CCWGG 1 cut(s) 316
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AleI CACNNNNGTG 1 cut(s) 188
AluBI AGCT 5 cut(s) 26, 69, 280, 301, 483
AluI AGCT 5 cut(s) 26, 69, 280, 301, 483
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 2 cut(s) 153, 320
ApeKI GCWGC 1 cut(s) 277
ArsI GACNNNNNNTTYG 4 cut(s) 14, 46, 204, 236
AspLEI GCGC 2 cut(s) 209, 476
AspS9I GGNCC 3 cut(s) 153, 320, 449
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 449
BbsI GAAGAC 2 cut(s) 46, 246
BbvI GCAGC 1 cut(s) 289
BceAI ACGGC 1 cut(s) 304
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 1 cut(s) 318
BfoI RGCGCY 2 cut(s) 210, 477
BisI GCNGC 2 cut(s) 278, 472
BlsI GCNGC 2 cut(s) 279, 473
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 3 cut(s) 153, 320, 449
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 183
BpiI GAAGAC 2 cut(s) 46, 246
BpuEI CTTGAG 1 cut(s) 266
BsaJI CCNNGG 4 cut(s) 317, 345, 395, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 4 cut(s) 317, 345, 395, 406
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 2 cut(s) 87, 231
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 1 cut(s) 28
BseXI GCAGC 1 cut(s) 289
BshFI GGCC 2 cut(s) 155, 322
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 2 cut(s) 155, 322
Bsp143I GATC 1 cut(s) 87
BspACI CCGC 4 cut(s) 166, 339, 420, 471
BspANI GGCC 2 cut(s) 155, 322
BspCNI CTCAG 2 cut(s) 86, 230
BspLI GGNNCC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 4 cut(s) 317, 345, 395, 406
BssMI GATC 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 345, 395
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 94, 449, 505
Bst6I CTCTTC 2 cut(s) 48, 68
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 73, 217
BstF5I GGATG 1 cut(s) 14
BstH2I RGCGCY 2 cut(s) 210, 477
BstHHI GCGC 2 cut(s) 209, 476
BstKTI GATC 1 cut(s) 90
BstMBI GATC 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 108, 480
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstV1I GCAGC 1 cut(s) 289
BstV2I GAAGAC 2 cut(s) 46, 246
BsuRI GGCC 2 cut(s) 155, 322
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 2 cut(s) 209, 476
Cfr13I GGNCC 3 cut(s) 153, 320, 449
CseI GACGC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 199
CviAII CATG 1 cut(s) 114
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 73, 217
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
Eam1104I CTCTTC 2 cut(s) 48, 68
EarI CTCTTC 2 cut(s) 48, 68
Eco130I CCWWGG 2 cut(s) 345, 395
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 345, 395
ErhI CCWWGG 2 cut(s) 345, 395
FaeI CATG 1 cut(s) 117
FaiI YATR 3 cut(s) 34, 115, 389
FaqI GGGAC 1 cut(s) 363
FatI CATG 1 cut(s) 113
FauI CCCGC 2 cut(s) 173, 427
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 278, 472
Fsp4HI GCNGC 2 cut(s) 278, 472
GlaI GCGC 2 cut(s) 208, 475
GluI GCNGC 2 cut(s) 278, 472
HaeII RGCGCY 2 cut(s) 210, 477
HaeIII GGCC 2 cut(s) 155, 322
HapII CCGG 1 cut(s) 431
HgaI GACGC 1 cut(s) 249
HhaI GCGC 2 cut(s) 209, 476
Hin1II CATG 1 cut(s) 117
Hin6I GCGC 2 cut(s) 207, 474
HinP1I GCGC 2 cut(s) 207, 474
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 3 cut(s) 131, 215, 391
HpaII CCGG 1 cut(s) 431
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 445
Hpy188I TCNGA 4 cut(s) 76, 130, 465, 501
Hpy8I GTNNAC 1 cut(s) 445
Hpy99I CGWCG 3 cut(s) 53, 98, 449
HpyAV CCTTC 3 cut(s) 355, 435, 481
HpyCH4III ACNGT 3 cut(s) 94, 449, 505
HpyCH4V TGCA 1 cut(s) 255
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 480
HpyF3I CTNAG 2 cut(s) 73, 217
Hsp92II CATG 1 cut(s) 117
HspAI GCGC 2 cut(s) 207, 474
Kzo9I GATC 1 cut(s) 87
LmnI GCTCC 3 cut(s) 306, 427, 466
LpnPI CCDG 8 cut(s) 94, 303, 326, 330, 359, 420, 444, 465
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 229
MalI GATC 1 cut(s) 89
MbiI CCGCTC 1 cut(s) 422
MboI GATC 1 cut(s) 87
MboII GAAGA 4 cut(s) 35, 46, 55, 251
MfeI CAATTG 1 cut(s) 270
MluCI AATT 1 cut(s) 270
MlyI GAGTC 2 cut(s) 209, 385
MmeI TCCRAC 1 cut(s) 488
MnlI CCTC 5 cut(s) 28, 49, 152, 416, 433
MslI CAYNNNNRTG 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 318
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 108, 480
NdeII GATC 1 cut(s) 87
NlaIII CATG 1 cut(s) 117
NlaIV GGNNCC 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 188
PfeI GAWTC 1 cut(s) 131
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 2 cut(s) 279, 473
PleI GAGTC 2 cut(s) 209, 385
PpsI GAGTC 2 cut(s) 209, 385
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 3 cut(s) 153, 320, 449
PstNI CAGNNNCTG 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 188
SalI GTCGAC 1 cut(s) 443
SatI GCNGC 2 cut(s) 278, 472
Sau3AI GATC 1 cut(s) 87
Sau96I GGNCC 3 cut(s) 153, 320, 449
SchI GAGTC 2 cut(s) 209, 385
ScrFI CCNGG 1 cut(s) 318
SetI ASST 8 cut(s) 28, 71, 282, 303, 309, 408, 444, 485
SfaNI GCATC 1 cut(s) 183
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 1 cut(s) 270
SsiI CCGC 4 cut(s) 166, 339, 420, 471
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 2 cut(s) 345, 395
TaaI ACNGT 3 cut(s) 94, 449, 505
TaqI TCGA 1 cut(s) 444
TasI AATT 1 cut(s) 270
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 1 cut(s) 359
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 322
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 1 cut(s) 359
Tth111I GACNNNGTC 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.