Rw6G034960

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012899
Physical Location & Seq
Reverse (-)
59505870 .. 59507039
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034960.1

Sequence Viewer

Length: 549 bp
ATGGCTGGGTTTTCATCAAGAGCTGCTTCTGTGCTTGTCGTGTTTCTCGTCTTGCTGAGCTTTTCAGAAGCCAGAGAGTTTTTGGTGGGAGGCAAAGCAGACGCATGGGCAGTCCCTTCTTCTGAATCCCAATCTCTCAACAAATGGGCTGAAAGCAAACGGTTCAACACCGGCGACATTCTCGTGTGGAAGTATGACAGTGCAAAAGACTCGGTCTTGCATGTGACCAAGGAAGACTATGTTAACTGCAACATGTCAAGCCCAATCAAGCAGTACAAGGATGGTGAGACCAAGATTGTGCTTGACAAACCAGGACCCTTCTATTTCATTAGCGGAACCAAGGACCACTGTGAAAAGGGACAGAAGCTCGTCGTTGTTGTTATGTCTCCCAGAAACAGGGCCTCTACCATCTCTCCGGCACCTTCTCCCGCTGTCGAGATCGACGGTCCTGCCGTTGCTCCGGCTCCGACTAGCATTGCTTCGCCTTTCAAGGCCGCTGGTGTCCTTGTTGTGGCACTAATGGGGTTTTCGGCAGCTCTCCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

182

Amino Acids

19.44

Weight (kDa)

8.5

Isoelectric Point (pI)

55.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 121 1.2e-26 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012899)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25060 AT4G31840 AT5G25090
fragaria_vesca FvH4_2g41150
malus_domestica MD08G1056100.v1.1 MD15G1040900.v1.1
prunus_persica Prupe.1G394200_v2.0.a1
pyrus_communis pycom08g04610 pycom15g03810
rosa_chinensis RchiOBHm_Chr6g0299381
rosa_laevigata RLG00000011437
rosa_roxburghii Rroxscaffold_7G00168740
rosa_rugosa Rorug06G0287700
rosa_samantha Rh6AG399300 Rh6BG407800 Rh6CG413600 Rh6DG400500
rosa_wichuraiana Rw6G034960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AciI CCGC 3 cut(s) 333, 429, 495
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 2 cut(s) 396, 511
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 2 cut(s) 166, 490
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 4 cut(s) 23, 60, 367, 536
AluI AGCT 4 cut(s) 23, 60, 367, 536
Alw26I GTCTC 2 cut(s) 281, 390
AoxI GGCC 2 cut(s) 399, 492
ApeKI GCWGC 2 cut(s) 23, 533
AspS9I GGNCC 4 cut(s) 314, 343, 399, 446
AsuHPI GGTGA 1 cut(s) 296
AvaII GGWCC 3 cut(s) 314, 343, 446
BanI GGYRCC 1 cut(s) 418
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 10, 545
BccI CCATC 2 cut(s) 275, 416
BceAI ACGGC 1 cut(s) 437
BcgI CGANNNNNNTGC 4 cut(s) 192, 226, 431, 465
BciT130I CCWGG 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 281, 390
BfaI CTAG 1 cut(s) 471
BisI GCNGC 3 cut(s) 24, 495, 534
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 3 cut(s) 25, 496, 535
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 3 cut(s) 314, 343, 446
BmgT120I GGNCC 4 cut(s) 314, 343, 399, 446
BmiI GGNNCC 4 cut(s) 316, 337, 420, 465
BmrFI CCNGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 228, 339
BsaXI ACNNNNNCTCC 2 cut(s) 397, 427
Bsc4I CCNNNNNNNGG 2 cut(s) 396, 511
Bse118I RCCGGY 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 228, 339
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 396, 511
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 47
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 2 cut(s) 10, 545
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 2 cut(s) 401, 494
BshNI GGYRCC 1 cut(s) 418
BsiSI CCGG 3 cut(s) 171, 416, 461
BslFI GGGAC 2 cut(s) 98, 372
BslI CCNNNNNNNGG 2 cut(s) 396, 511
BsmAI GTCTC 2 cut(s) 281, 390
BsmFI GGGAC 2 cut(s) 98, 372
BsnI GGCC 2 cut(s) 401, 494
Bso31I GGTCTC 1 cut(s) 281
Bsp143I GATC 1 cut(s) 438
Bsp1720I GCTNAGC 1 cut(s) 56
BspACI CCGC 3 cut(s) 333, 429, 495
BspANI GGCC 2 cut(s) 401, 494
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 4 cut(s) 316, 337, 420, 465
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 228, 339
BssMI GATC 1 cut(s) 438
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 228, 339
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 4 cut(s) 162, 200, 350, 446
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 1 cut(s) 441
BstMAI GTCTC 2 cut(s) 281, 390
BstMBI GATC 1 cut(s) 438
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 2 cut(s) 224, 256
BstSCI CCNGG 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 10, 545
BstV2I GAAGAC 1 cut(s) 240
BsuRI GGCC 2 cut(s) 401, 494
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 2 cut(s) 205, 346
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 4 cut(s) 314, 343, 399, 446
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 274
CviAII CATG 3 cut(s) 105, 221, 253
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 56
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 228, 339
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 3 cut(s) 314, 343, 446
EcoO109I RGGNCCY 2 cut(s) 314, 399
EcoRII CCWGG 1 cut(s) 310
EcoT14I CCWWGG 2 cut(s) 228, 339
ErhI CCWWGG 2 cut(s) 228, 339
FaeI CATG 3 cut(s) 108, 224, 256
FaiI YATR 6 cut(s) 106, 195, 222, 240, 254, 383
FalI AAGNNNNNCTT 2 cut(s) 10, 42
FaqI GGGAC 2 cut(s) 98, 372
FatI CATG 3 cut(s) 104, 220, 252
FauI CCCGC 1 cut(s) 436
Fnu4HI GCNGC 3 cut(s) 24, 495, 534
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 3 cut(s) 24, 495, 534
FspBI CTAG 1 cut(s) 471
GluI GCNGC 3 cut(s) 24, 495, 534
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 2 cut(s) 401, 494
HapII CCGG 3 cut(s) 171, 416, 461
HgaI GACGC 1 cut(s) 110
Hin1II CATG 3 cut(s) 108, 224, 256
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 2 cut(s) 125, 209
HpaI GTTAAC 1 cut(s) 244
HpaII CCGG 3 cut(s) 171, 416, 461
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 4 cut(s) 67, 124, 468, 548
Hpy188III TCNNGA 2 cut(s) 18, 436
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 2 cut(s) 374, 446
HpyAV CCTTC 3 cut(s) 126, 328, 432
HpyCH4III ACNGT 4 cut(s) 162, 200, 350, 446
HpyCH4V TGCA 3 cut(s) 203, 220, 249
HpyF3I CTNAG 1 cut(s) 56
Hsp92II CATG 3 cut(s) 108, 224, 256
KspAI GTTAAC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 463, 469
Lsp1109I GCAGC 2 cut(s) 10, 545
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 1 cut(s) 223
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 2 cut(s) 111, 245
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 491
MnlI CCTC 2 cut(s) 83, 412
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 182
MspA1I CMGCKG 2 cut(s) 431, 497
MspI CCGG 3 cut(s) 171, 416, 461
MspR9I CCNGG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 312
NdeII GATC 1 cut(s) 438
NlaIII CATG 3 cut(s) 108, 224, 256
NlaIV GGNNCC 4 cut(s) 316, 337, 420, 465
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 2 cut(s) 224, 256
PciI ACATGT 1 cut(s) 252
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 450
PfeI GAWTC 1 cut(s) 125
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 3 cut(s) 25, 496, 535
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PpuMI RGGWCCY 1 cut(s) 314
PscI ACATGT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 4 cut(s) 316, 337, 420, 465
PspPI GGNCC 4 cut(s) 314, 343, 399, 446
PspPPI RGGWCCY 1 cut(s) 314
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 3 cut(s) 24, 495, 534
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 4 cut(s) 314, 343, 399, 446
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 312
SetI ASST 5 cut(s) 25, 62, 369, 424, 538
SgrAI CRCCGGYG 1 cut(s) 170
SinI GGWCC 3 cut(s) 314, 343, 446
SmiMI CAYNNNNRTG 1 cut(s) 182
SsiI CCGC 3 cut(s) 333, 429, 495
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 2 cut(s) 228, 339
TaaI ACNGT 4 cut(s) 162, 200, 350, 446
TaqI TCGA 2 cut(s) 435, 441
TaqII GACCGA 1 cut(s) 202
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 497
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 205, 353
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 23, 533
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 316
TspRI CASTG 2 cut(s) 205, 353
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 3 cut(s) 314, 343, 446
XceI RCATGY 2 cut(s) 224, 256
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.