MD15G1040900.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012899
Physical Location & Seq
Forward (+)
2875152 .. 2876153
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1040900.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGCTGGCTTTTCATCCTCAAAAGCTCTGTCATACTCCTCTCTTCTCGTGGTCTTTCTCCTCTTCAGCTTCTCGGAAGCCAGAGAGTTCGCCGTCGGCGGCAAGAATGGCTCATGGGCAGTCCCCTCCTCTGAATCCCAATCCCTCAACAAATGGGCTGAAAGCACCCGCTTTCGCGTCGGCGACACTCTTGTGTGGAAGTATGACAGCGCCAAAGACTCAGTGTTGCGAGTGACGAAAGAAGACTACACCAACTGCAATGCTTCAAATCCTATTGAGCAGTACAAGGACGGCGAGACCAAGCTCCACCTCGACCAGCCGGGGCCTTACTACTTCATCAGCGGAACCAAGGGACACTGCGAGAAAGGGCAGAAGCTGGTCGTGGTTGTTATGACTCCAAGGAAGCACCTCGGTATCTCTCCCGCTCCTTCTCCGGCTGGGGAGGTTGACGGTCCTGCTGTTGCTCCTACCAGCGGTGCTACAAGCTTGCAGGGTGGTGGTCTTCTGACTCTGGTGGCATTGGGAGTTTTTACTCTCTGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

182

Amino Acids

19.43

Weight (kDa)

7.75

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 39 - 124 2.3e-28 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012899)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25060 AT4G31840 AT5G25090
fragaria_vesca FvH4_2g41150
malus_domestica MD08G1056100.v1.1 MD15G1040900.v1.1
prunus_persica Prupe.1G394200_v2.0.a1
pyrus_communis pycom08g04610 pycom15g03810
rosa_chinensis RchiOBHm_Chr6g0299381
rosa_laevigata RLG00000011437
rosa_roxburghii Rroxscaffold_7G00168740
rosa_rugosa Rorug06G0287700
rosa_samantha Rh6AG399300 Rh6BG407800 Rh6CG413600 Rh6DG400500
rosa_wichuraiana Rw6G034960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 425
AccII CGCG 1 cut(s) 177
AciI CCGC 5 cut(s) 99, 169, 342, 423, 474
AcuI CTGAAG 1 cut(s) 49
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 1 cut(s) 267
AleI CACNNNNGTG 1 cut(s) 191
AluBI AGCT 5 cut(s) 26, 69, 304, 376, 486
AluI AGCT 5 cut(s) 26, 69, 304, 376, 486
Alw26I GTCTC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 1 cut(s) 323
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 323, 452
AsuC2I CCSGG 1 cut(s) 321
AvaII GGWCC 1 cut(s) 452
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BauI CACGAG 1 cut(s) 47
BbsI GAAGAC 2 cut(s) 249, 494
BceAI ACGGC 2 cut(s) 77, 307
BcnI CCSGG 1 cut(s) 321
BcoDI GTCTC 1 cut(s) 290
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 2 cut(s) 323, 452
BmiI GGNNCC 2 cut(s) 324, 346
BmrFI CCNGG 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 249, 494
BpuMI CCSGG 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 4 cut(s) 320, 348, 398, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse3DI GCAATG 1 cut(s) 265
BseDI CCNNGG 4 cut(s) 320, 348, 398, 409
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 234
BseRI GAGGAG 3 cut(s) 28, 50, 118
BseYI CCCAGC 1 cut(s) 437
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 325
BsiSI CCGG 2 cut(s) 320, 434
BslFI GGGAC 2 cut(s) 107, 366
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 2 cut(s) 107, 366
BsnI GGCC 1 cut(s) 325
Bso31I GGTCTC 1 cut(s) 290
BspACI CCGC 5 cut(s) 99, 169, 342, 423, 474
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 1 cut(s) 233
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 324, 346
BspTNI GGTCTC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 265
BssECI CCNNGG 4 cut(s) 320, 348, 398, 409
BssSI CACGAG 1 cut(s) 47
BssT1I CCWWGG 2 cut(s) 348, 398
Bst2BI CACGAG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 2 cut(s) 48, 68
BstC8I GCNNGC 2 cut(s) 7, 488
BstDEI CTNAG 1 cut(s) 220
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 290
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 319
BstUI CGCG 1 cut(s) 177
BstV2I GAAGAC 2 cut(s) 249, 494
BsuRI GGCC 1 cut(s) 325
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 2 cut(s) 228, 355
Cac8I GCNNGC 2 cut(s) 7, 488
CaiI CAGNNNCTG 1 cut(s) 376
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 323, 452
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 283
CviAII CATG 1 cut(s) 114
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 220
Eam1104I CTCTTC 2 cut(s) 48, 68
EarI CTCTTC 2 cut(s) 48, 68
Eco130I CCWWGG 2 cut(s) 348, 398
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 348, 398
ErhI CCWWGG 2 cut(s) 348, 398
FaeI CATG 1 cut(s) 117
FaiI YATR 4 cut(s) 34, 115, 204, 392
FaqI GGGAC 2 cut(s) 107, 366
FatI CATG 1 cut(s) 113
FauI CCCGC 2 cut(s) 176, 430
Fnu4HI GCNGC 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 100
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 100
GsaI CCCAGC 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 325
HapII CCGG 2 cut(s) 320, 434
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 212
Hin1II CATG 1 cut(s) 117
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 4 cut(s) 134, 218, 394, 508
HpaII CCGG 2 cut(s) 320, 434
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 3 cut(s) 76, 133, 507
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 2 cut(s) 98, 182
HpyAV CCTTC 1 cut(s) 438
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4V TGCA 2 cut(s) 258, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 1 cut(s) 220
Hsp92II CATG 1 cut(s) 117
HspAI GCGC 1 cut(s) 210
LmnI GCTCC 3 cut(s) 309, 430, 469
MaeIII GTNAC 1 cut(s) 232
MbiI CCGCTC 1 cut(s) 425
MboII GAAGA 4 cut(s) 35, 55, 254, 494
MlyI GAGTC 3 cut(s) 212, 388, 502
MnlI CCTC 9 cut(s) 28, 49, 71, 136, 139, 155, 320, 419, 436
MslI CAYNNNNRTG 1 cut(s) 191
MspA1I CMGCKG 2 cut(s) 342, 474
MspI CCGG 2 cut(s) 320, 434
MspR9I CCNGG 1 cut(s) 321
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 108
NciI CCSGG 1 cut(s) 321
NlaIII CATG 1 cut(s) 117
NlaIV GGNNCC 2 cut(s) 324, 346
NmuCI GTSAC 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 191
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 3 cut(s) 212, 388, 502
PpsI GAGTC 3 cut(s) 212, 388, 502
PspFI CCCAGC 1 cut(s) 437
PspN4I GGNNCC 2 cut(s) 324, 346
PspPI GGNCC 2 cut(s) 323, 452
PstNI CAGNNNCTG 1 cut(s) 376
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 191
SatI GCNGC 1 cut(s) 100
Sau96I GGNCC 2 cut(s) 323, 452
SchI GAGTC 3 cut(s) 212, 388, 502
ScrFI CCNGG 1 cut(s) 321
SetI ASST 8 cut(s) 28, 71, 306, 312, 378, 411, 447, 488
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 1 cut(s) 191
SsiI CCGC 5 cut(s) 99, 169, 342, 423, 474
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 2 cut(s) 348, 398
TaaI ACNGT 1 cut(s) 452
TaqI TCGA 1 cut(s) 312
TatI WGTACW 1 cut(s) 282
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 1 cut(s) 134
TscAI CASTG 2 cut(s) 228, 362
TseFI GTSAC 1 cut(s) 232
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 325
TspRI CASTG 2 cut(s) 228, 362
VpaK11BI GGWCC 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.