Rh6DG400500

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012899
Physical Location & Seq
Reverse (-)
58953998 .. 58955139
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG400500.1

Sequence Viewer

Length: 558 bp
ATGGCTGGGTTTTCATCAATAGCTGCTGCTTCTGTGCTTGTCGTGTTTCTCGTCATGTTGATCAGCTTTTCAGAAGCCAGAGAGTTTTTGGTGGGAGGCAAAGCAGACGCATGGGCAGTCCCTTCCTCTGAATCCCAATCTCTCAACAAATGGGCCGAAAGCAAACGGTTCAACACCGGCGACATTCTCGTGTGGAAGTATGACAGTGCAAAAGACTCAGTCTTGCATGTGACCAAGGAAGACTATGTTAACTGCAACATGTCAAGCCCAATCAAGCAGTACAAGGATGGTGAGACCAAGATTGTGCTTGACAAACCAGGACCCTTCTATTTCATTAGCGGAACCAAGGACCACTGTGAAAAGGGACAGAAGCTCGTCGTTGTCGTTATGTCTCCCAGAAACAGGGCCTCTACCATCTCTCCGGCACCTTCTCCCGCGGTCGAGATCGACGGTCCTGCCGTTGCTCCGGCTCCGACTAGCACTGCTTCGCCTTTCAAGGCCGCTGGTGTCCTTGTTGTGGCGGCACTAATGGGGTTTTCGGCAGCTCTCCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

185

Amino Acids

19.66

Weight (kDa)

7.73

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 38 - 123 1.2e-26 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012899)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25060 AT4G31840 AT5G25090
fragaria_vesca FvH4_2g41150
malus_domestica MD08G1056100.v1.1 MD15G1040900.v1.1
prunus_persica Prupe.1G394200_v2.0.a1
pyrus_communis pycom08g04610 pycom15g03810
rosa_chinensis RchiOBHm_Chr6g0299381
rosa_laevigata RLG00000011437
rosa_roxburghii Rroxscaffold_7G00168740
rosa_rugosa Rorug06G0287700
rosa_samantha Rh6AG399300 Rh6BG407800 Rh6CG413600 Rh6DG400500
rosa_wichuraiana Rw6G034960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 424
AccII CGCG 1 cut(s) 437
AciI CCGC 5 cut(s) 339, 435, 437, 501, 521
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 2 cut(s) 402, 517
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 2 cut(s) 172, 496
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 4 cut(s) 23, 66, 373, 545
AluI AGCT 4 cut(s) 23, 66, 373, 545
Alw26I GTCTC 2 cut(s) 287, 396
AoxI GGCC 3 cut(s) 153, 405, 498
ApeKI GCWGC 3 cut(s) 23, 26, 542
AspS9I GGNCC 5 cut(s) 153, 320, 349, 405, 452
AsuHPI GGTGA 1 cut(s) 302
AvaII GGWCC 3 cut(s) 320, 349, 452
BanI GGYRCC 1 cut(s) 424
BauI CACGAG 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 10, 13, 554
BccI CCATC 2 cut(s) 281, 422
BceAI ACGGC 1 cut(s) 443
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 287, 396
BfaI CTAG 1 cut(s) 477
BisI GCNGC 5 cut(s) 24, 27, 501, 522, 543
BlsI GCNGC 5 cut(s) 25, 28, 502, 523, 544
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 3 cut(s) 320, 349, 452
BmgT120I GGNCC 5 cut(s) 153, 320, 349, 405, 452
BmiI GGNNCC 4 cut(s) 322, 343, 426, 471
BmrFI CCNGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 3 cut(s) 234, 345, 435
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 517
Bse118I RCCGGY 1 cut(s) 176
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 3 cut(s) 234, 345, 435
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 2 cut(s) 402, 517
BseMII CTCAG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 3 cut(s) 10, 13, 554
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 3 cut(s) 155, 407, 500
BshNI GGYRCC 1 cut(s) 424
BsiEI CGRYCG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 177, 422, 467
BslFI GGGAC 2 cut(s) 104, 378
BslI CCNNNNNNNGG 2 cut(s) 402, 517
BsmAI GTCTC 2 cut(s) 287, 396
BsmFI GGGAC 2 cut(s) 104, 378
BsnI GGCC 3 cut(s) 155, 407, 500
Bso31I GGTCTC 1 cut(s) 287
Bsp143I GATC 2 cut(s) 60, 444
BspACI CCGC 5 cut(s) 339, 435, 437, 501, 521
BspANI GGCC 3 cut(s) 155, 407, 500
BspCNI CTCAG 1 cut(s) 230
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 4 cut(s) 322, 343, 426, 471
BspT107I GGYRCC 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 234, 345, 435
BssMI GATC 2 cut(s) 60, 444
BssSI CACGAG 1 cut(s) 188
BssT1I CCWWGG 2 cut(s) 234, 345
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 4 cut(s) 168, 206, 356, 452
BstDEI CTNAG 1 cut(s) 217
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 437
BstKTI GATC 2 cut(s) 63, 447
BstMAI GTCTC 2 cut(s) 287, 396
BstMBI GATC 2 cut(s) 60, 444
BstMCI CGRYCG 1 cut(s) 441
BstNI CCWGG 1 cut(s) 318
BstNSI RCATGY 2 cut(s) 230, 262
BstSCI CCNGG 1 cut(s) 316
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 3 cut(s) 10, 13, 554
BstV2I GAAGAC 1 cut(s) 246
BsuRI GGCC 3 cut(s) 155, 407, 500
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 211, 352, 480
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 5 cut(s) 153, 320, 349, 405, 452
Cfr42I CCGCGG 1 cut(s) 438
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 280
CviAII CATG 4 cut(s) 55, 111, 227, 259
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 1 cut(s) 217
DpnI GATC 2 cut(s) 62, 446
DpnII GATC 2 cut(s) 60, 444
Eco130I CCWWGG 2 cut(s) 234, 345
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 3 cut(s) 320, 349, 452
EcoO109I RGGNCCY 2 cut(s) 320, 405
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 234, 345
ErhI CCWWGG 2 cut(s) 234, 345
FaeI CATG 4 cut(s) 58, 114, 230, 262
FaiI YATR 7 cut(s) 56, 112, 201, 228, 246, 260, 389
FaqI GGGAC 2 cut(s) 104, 378
FatI CATG 4 cut(s) 54, 110, 226, 258
FauI CCCGC 1 cut(s) 442
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 5 cut(s) 24, 27, 501, 522, 543
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 5 cut(s) 24, 27, 501, 522, 543
FspBI CTAG 1 cut(s) 477
GluI GCNGC 5 cut(s) 24, 27, 501, 522, 543
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 155, 407, 500
HapII CCGG 3 cut(s) 177, 422, 467
HgaI GACGC 1 cut(s) 116
Hin1II CATG 4 cut(s) 58, 114, 230, 262
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 2 cut(s) 131, 215
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 3 cut(s) 177, 422, 467
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 73, 130, 474, 557
Hpy188III TCNNGA 1 cut(s) 442
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 2 cut(s) 380, 452
HpyAV CCTTC 3 cut(s) 132, 334, 438
HpyCH4III ACNGT 4 cut(s) 168, 206, 356, 452
HpyCH4V TGCA 3 cut(s) 209, 226, 255
HpyF3I CTNAG 1 cut(s) 217
Hsp92II CATG 4 cut(s) 58, 114, 230, 262
Ksp22I TGATCA 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 250
KspI CCGCGG 1 cut(s) 438
Kzo9I GATC 2 cut(s) 60, 444
LmnI GCTCC 2 cut(s) 469, 475
Lsp1109I GCAGC 3 cut(s) 10, 13, 554
MaeI CTAG 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 62, 446
MboI GATC 2 cut(s) 60, 444
MboII GAAGA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 497
MnlI CCTC 3 cut(s) 89, 136, 418
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 437, 503
MspI CCGG 3 cut(s) 177, 422, 467
MspR9I CCNGG 1 cut(s) 318
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 437
NdeII GATC 2 cut(s) 60, 444
NlaIII CATG 4 cut(s) 58, 114, 230, 262
NlaIV GGNNCC 4 cut(s) 322, 343, 426, 471
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 2 cut(s) 230, 262
PciI ACATGT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 381, 456
PfeI GAWTC 1 cut(s) 131
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 5 cut(s) 25, 28, 502, 523, 544
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PpuMI RGGWCCY 1 cut(s) 320
PscI ACATGT 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 4 cut(s) 322, 343, 426, 471
PspPI GGNCC 5 cut(s) 153, 320, 349, 405, 452
PspPPI RGGWCCY 1 cut(s) 320
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 188
SacII CCGCGG 1 cut(s) 438
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 5 cut(s) 24, 27, 501, 522, 543
Sau3AI GATC 2 cut(s) 60, 444
Sau96I GGNCC 5 cut(s) 153, 320, 349, 405, 452
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 318
SetI ASST 5 cut(s) 25, 68, 375, 430, 547
Sfr303I CCGCGG 1 cut(s) 438
SgrAI CRCCGGYG 1 cut(s) 176
SgrBI CCGCGG 1 cut(s) 438
SinI GGWCC 3 cut(s) 320, 349, 452
SmiMI CAYNNNNRTG 1 cut(s) 188
SsiI CCGC 5 cut(s) 339, 435, 437, 501, 521
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 2 cut(s) 234, 345
TaaI ACNGT 4 cut(s) 168, 206, 356, 452
TaqI TCGA 2 cut(s) 441, 447
TatI WGTACW 1 cut(s) 279
TauI GCSGC 2 cut(s) 503, 524
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 3 cut(s) 211, 359, 487
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 3 cut(s) 23, 26, 542
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 322
TspRI CASTG 3 cut(s) 211, 359, 487
Tth111I GACNNNGTC 1 cut(s) 218
VpaK11BI GGWCC 3 cut(s) 320, 349, 452
XceI RCATGY 2 cut(s) 230, 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.