MD08G1056200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006740
Physical Location & Seq
Forward (+)
4368839 .. 4369141
303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1056200.v1.1.491

Sequence Viewer

Length: 303 bp
ATGGCGGGGGACGAAGATTGGAGGAAAACGGCGGAGACGACAAAAATGAGTCCGGAAGAAGTGAAGAGAGCTGGACTGCAGGGATCACAGAGGCCTCCCGGTCATAACCCCGGCGGTCTTCTTCACCAGCGACGTCTCGTTCCCCTTGGCCCTACCGCCTTGGCATTCGCCGGCTCCCTCATTGTTGGTACCATCGACTACTTCACCCTTTACGCCCGCAAAAAGCCCGAAGCCACCGCCGGAGACGTTGCCAGGGTCTCCGCCAATGTTGCCAGCCCCGAGGACACTCGCCCAAGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

10.77

Weight (kDa)

9.77

Isoelectric Point (pI)

61.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 136
Acc65I GGTACC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 188
AccIII TCCGGA 1 cut(s) 52
AciI CCGC 7 cut(s) 5, 32, 114, 156, 217, 237, 261
AclWI GGATC 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 1 cut(s) 190
AjnI CCWGG 1 cut(s) 251
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw26I GTCTC 4 cut(s) 29, 140, 237, 262
AlwI GGATC 1 cut(s) 91
Ama87I CYCGRG 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 52
AoxI GGCC 2 cut(s) 92, 148
Asp718I GGTACC 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 149
AsuC2I CCSGG 2 cut(s) 99, 111
AsuHPI GGTGA 2 cut(s) 116, 196
AvaI CYCGRG 1 cut(s) 278
BanI GGYRCC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 110
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 45
BciT130I CCWGG 1 cut(s) 253
BcnI CCSGG 2 cut(s) 99, 111
BcoDI GTCTC 4 cut(s) 29, 140, 237, 262
BfmI CTRYAG 1 cut(s) 77
Bme1390I CCNGG 3 cut(s) 99, 111, 253
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 2 cut(s) 175, 190
BmrFI CCNGG 3 cut(s) 99, 111, 253
BpiI GAAGAC 1 cut(s) 110
BpuMI CCSGG 2 cut(s) 99, 111
BsaHI GRCGYC 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 6 cut(s) 109, 145, 159, 252, 279, 293
BsaWI WCCGGW 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 170
BseAI TCCGGA 1 cut(s) 52
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 6 cut(s) 109, 145, 159, 252, 279, 293
BshFI GGCC 2 cut(s) 94, 150
BshNI GGYRCC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 5 cut(s) 53, 99, 111, 171, 240
BslFI GGGAC 1 cut(s) 23
BsmAI GTCTC 4 cut(s) 29, 140, 237, 262
BsmBI CGTCTC 3 cut(s) 29, 140, 237
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 2 cut(s) 94, 150
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 278
Bsp13I TCCGGA 1 cut(s) 52
Bsp143I GATC 1 cut(s) 83
BspACI CCGC 7 cut(s) 5, 32, 114, 156, 217, 237, 261
BspANI GGCC 2 cut(s) 94, 150
BspEI TCCGGA 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 175, 190
BspMAI CTGCAG 1 cut(s) 81
BspPI GGATC 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 6 cut(s) 109, 145, 159, 252, 279, 293
BssMI GATC 1 cut(s) 83
BssNI GRCGYC 1 cut(s) 133
BssT1I CCWWGG 3 cut(s) 145, 159, 293
Bst2UI CCWGG 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 172, 217, 274
BstKTI GATC 1 cut(s) 86
BstMAI GTCTC 4 cut(s) 29, 140, 237, 262
BstMBI GATC 1 cut(s) 83
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 3 cut(s) 97, 109, 251
BstSFI CTRYAG 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 2 cut(s) 94, 150
Cac8I GCNNGC 3 cut(s) 172, 217, 274
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 189
CviJI RGCY 7 cut(s) 71, 94, 150, 174, 226, 233, 276
CviKI_1 RGCY 7 cut(s) 71, 94, 150, 174, 226, 233, 276
CviQI GTAC 1 cut(s) 189
DpnI GATC 1 cut(s) 85
DpnII GATC 1 cut(s) 83
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 2 cut(s) 47, 250
Eco130I CCWWGG 3 cut(s) 145, 159, 293
Eco147I AGGCCT 1 cut(s) 94
Eco31I GGTCTC 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 251
EcoT14I CCWWGG 3 cut(s) 145, 159, 293
ErhI CCWWGG 3 cut(s) 145, 159, 293
Esp3I CGTCTC 3 cut(s) 29, 140, 237
FaiI YATR 1 cut(s) 105
FaqI GGGAC 1 cut(s) 23
FauI CCCGC 1 cut(s) 224
HaeIII GGCC 2 cut(s) 94, 150
HapII CCGG 5 cut(s) 53, 99, 111, 171, 240
Hin1I GRCGYC 1 cut(s) 133
HinfI GANTC 1 cut(s) 49
HpaII CCGG 5 cut(s) 53, 99, 111, 171, 240
HphI GGTGA 2 cut(s) 116, 196
Hpy188III TCNNGA 1 cut(s) 53
Hpy99I CGWCG 1 cut(s) 135
HpyCH4IV ACGT 2 cut(s) 133, 246
HpyCH4V TGCA 1 cut(s) 79
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpySE526I ACGT 2 cut(s) 133, 246
Hsp92I GRCGYC 1 cut(s) 133
Kpn2I TCCGGA 1 cut(s) 52
KpnI GGTACC 1 cut(s) 192
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Kzo9I GATC 1 cut(s) 83
LmnI GCTCC 1 cut(s) 179
MaeII ACGT 2 cut(s) 133, 246
MalI GATC 1 cut(s) 85
MboI GATC 1 cut(s) 83
MboII GAAGA 5 cut(s) 26, 68, 76, 110, 113
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 5 cut(s) 15, 84, 105, 188, 274
MroI TCCGGA 1 cut(s) 52
MroNI GCCGGC 1 cut(s) 170
MspI CCGG 5 cut(s) 53, 99, 111, 171, 240
MspR9I CCNGG 3 cut(s) 99, 111, 253
Mva1269I GAATGC 1 cut(s) 164
MvaI CCWGG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 269
NaeI GCCGGC 1 cut(s) 172
NciI CCSGG 2 cut(s) 99, 111
NdeII GATC 1 cut(s) 83
NgoMIV GCCGGC 1 cut(s) 170
NlaIV GGNNCC 2 cut(s) 175, 190
PceI AGGCCT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PctI GAATGC 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 2 cut(s) 175, 190
PspPI GGNCC 1 cut(s) 149
PstI CTGCAG 1 cut(s) 81
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
Sau3AI GATC 1 cut(s) 83
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 3 cut(s) 99, 111, 253
SetI ASST 3 cut(s) 73, 136, 249
SfcI CTRYAG 1 cut(s) 77
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 7 cut(s) 5, 32, 114, 156, 217, 237, 261
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 3 cut(s) 97, 109, 251
StyI CCWWGG 3 cut(s) 145, 159, 293
TaiI ACGT 2 cut(s) 136, 249
TaqI TCGA 1 cut(s) 195
ZraI GACGTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.