MD08G1058600.v1.1

Electron transfer DM13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014477
Physical Location & Seq
Forward (+)
4668346 .. 4671786
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1058600.v1.1.491

Sequence Viewer

Length: 2730 bp
ATGCCTCGCAAACCCAATTTCCTAAGGTTTCTCTCCGTCCTCCTCCTCCTCCTCATACTCTGCCATGCCGATCCGGTCAGTTCCGATTGCCCCAAAACCAGCCCCCTTGTCAACTCCGAATCCGAATTCAAAATGCTCCAGCACCAGCTCCGCGGATCGATTCGGATAATCGATGATTGCTCCTTCAAGGTCTCCGATTTCGACATGCTCCCTGGCTCCGACGTCCACTGGTGGGCCGCCGCCGGCCCCGACTTTACTAATTTGACCGCCGGCTTCGTCGTCTCCGATCAGAAGCTCAACGAGACGTACAAAAGCGACAGCTTTATCGTGCGCTTGAAGGACAATGTCACCTGGGATCAGATCCAAGTCCTCGCCGTGTGGGACCTCCCCACCGCCTCCGACTTCGGGCACGTAATTCTCGGGGATTTCAAAAACGGGTCGTCTGGTTCGGCTCCTTCCCCGTCGCCGTCGAGTGGAACCGATTCAGGGAACGGAACGGGTCGGGTTCGGGTTCATACCGAGCCGACTATGCTGGAAAATTGCAAGGTTTTGTCGAAGAATTATAGGGTTCGATGGACGTTGAGTGCTGAGGAGAATCTGATCGATATCGGATTGGAGGCCGCCACCGGGACCATGAATTACATGGCTTTCGGGTGGGCCAACCCGAATTCGACTTCCGAGCTCATGCTCGGAGCTGATGTCGCCGTCGCGGGGTTCAAAGAGGATGGCATGGCGTTTGTGAACGATTTTTACATCACAAAGTACAGTGAGTGCACCTTGTACAAGGACGGCGAGGCGAAGGGGGTTTGCCCCGATACCAGATACGAAGGGTCCGGCCCAAATGGTGGCGAGGTGAACAATACCAAATTGGTTTACGGGCAGAGGAGGGATGCCGTGTCGTTTATTAGGTACCAGAGGCCGTTGGTTTCCAATGACAAGAAGTACGATTTGGCGGTGAATTATACGGAGAAGATGAAGGTCATTTGGGCATTAGGGCCGATTAGGCCACCAGATCTTCTTCAGCCGTATTATCTTCCCCAGAACCATGGTGGACCGAAGAGTGTGGTTTTCGGTCATTTGGTGCTCAATGTTTCTGAAAATGTGAATGACTGTTTGGGTCCTTTAGATGCAGATGAAAAGGAGGATCAGCATCTGATTATCGCCGATGCCAAGGCCCCGCTCGTAGTTACTTCTGACCCAGCATTACATTATCCAGACCCGCCAAACCCTTCGAAGGTTTTGTACATTAACAAGAAAGAGGCTCCGATGTTGAGAGTGGAAAGAGGGGTGCCCGTCAAGTTTTCAATACAAGCAGGGCATGATGTCGCGATGTACATCACTTCCGACCCGCTTGGTGGGAATGCTACACTAAGGAATTCTACCGAGACTATTTATGCAGGAGGGCCTGAAGCTCAAGGAGTTCAAGCCAAACCTATGGAATTGGTTTGGGCACCGGATAGGAATACCCCGGACCAAGTATACTATCAATCTCTTTACGAGCAGAAAATGGGTTGGAAAGTGCAGGTGGTTGACGGAGGTCTGCCTGATATGTATAATAACAGTGTCGTTTTGGATGATCAGCAAGTTACCTTGTTTTGGACATTGTCAGAACACTCAATATCTATTGCAGTTCGTGGTGAGAAGAAGAGCGGTTTTTTGGCAATAGGGTTTGGCAGAGGAATGGTGAACAGCTATTCCTATGTGGGTTGGATTGATAATATTGGTAAAGGGCGGGTAAACACTTACTGGATTGATGGAAGGGATGCTTCGAGTATACATCCAACAACTGAGAATTTGACTTATGTGAGGTGCAAGTCAGAAAATGGCATCATTACATTCGAGTTCAGTCGTCCTTTGAAACCGTCGTGTGGTAAGAGTGATAAACCGGAGTGTAAAAACATAATTGATCCCACTACTCCGCTTAAAGTTGTTTGGGCAATGGGTACTGCATGGACAGATGAGAATCTAAGTGAACAAAACATGCATTTTGTTACGAGCAGTAGGCCTATTAGGGTGCTGCTTATGCGTGGTTCTGCAGAGGCAGAGCAGGATTTACAGCCGGTATTAGCTGTACATGGGTTTATGATGTTTCTCGCTTGGGGTATCTTGCTTCCTGGTGGAATACTGTCGGCTAGATACTTGAAACATGTTAAGGGTGATGGTTGGTTCAAGCTTCATGTTTACTTGCAGTACTCAGGTTTGGCAATCATCCTACTTGCTGTTCTCTTTGCTGTTGCTGAGCTTAGGGGTTTCTTTGTCAGCTCATTACATGTTAAGTTTGGGATGGCTGCGTTATTTTTGGTCTGTATACAACCTGTGAATGCATACGTAAGGCCCAAAAGACCGGCTCATGGAGAGGAGGTTTCCTCTAAAAGGATTCTCTGGGAGTACTTTCATGTGATCGGTGGGAGATGCGCCTTTGTTTTAGGAATTGCCGCACTTTTCAGCGGAATGAAGCATTTAGGAGATAGATATGACGCTGAAAATGTTCACGGGCTTACTTGGGCTTTGATAATTTGGTTCTTGATGGGTGCATTGATCGTTTTGTATCTGGAATACCGTGAAAAACAACAAAGGAGGGATAGGAGTTTTGGAAGAAGCAATTGGGTGCTGGGGAACCTTGAGGAAGATGACTCTGTTGATCTGTTGAGCCCAAACGGAATACACTCAGAGAAAGAATCGCAAACGTCAGGAAGAATGGAAGTTCAATTAGAGCCTCTGAACAGATAG

Protein Analysis

910

Amino Acids

101.15

Weight (kDa)

5.97

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DM13 PF10517 42 - 139 2.1e-15 Electron transfer DM13
DOMON PF03351 185 - 332 3.6e-15 DOMON domain
At5g54830 PF25489 386 - 511 5.5e-71 At5g54830-like domain
DOMON PF03351 525 - 648 1.1e-24 DOMON domain
Cytochrom_C_asm PF01578 689 - 858 1.1e-06 Cytochrome C assembly protein
Cytochrom_B561 PF03188 691 - 819 1.3e-06 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54830
malus_domestica MD08G1058600.v1.1 MD15G1037900.v1.1
prunus_persica Prupe.1G391800_v2.0.a1
pyrus_communis pycom08g04850 pycom15g03510
rosa_chinensis RchiOBHm_Chr6g0299641
rosa_laevigata RLG00000011400
rosa_multiflora Rmu_co8233929.1_g000001 Rmu_co8458799.1_g000001 Rmu_sc0002895.1_g000005
rosa_roxburghii Rroxscaffold_7G00168480
rosa_rugosa Rorug06G0291300
rosa_samantha Rh6BG410500 Rh6CG416200 Rh6DG403400
rosa_wichuraiana Rw6G035190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1513
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 1513
Acc65I GGTACC 1 cut(s) 909
AccB1I GGYRCC 3 cut(s) 909, 1288, 1450
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 2 cut(s) 1180, 1650
AccI GTMKAC 3 cut(s) 1479, 1774, 2308
AccII CGCG 3 cut(s) 153, 710, 1328
AclWI GGATC 6 cut(s) 65, 163, 355, 363, 1152, 1901
AcsI RAATTY 4 cut(s) 125, 667, 1375, 1792
AcuI CTGAAG 2 cut(s) 1004, 1428
AcyI GRCGYC 1 cut(s) 222
AflIII ACRYGT 2 cut(s) 2146, 2269
AjnI CCWGG 3 cut(s) 211, 350, 2113
AjuI GAANNNNNNNTTGG 6 cut(s) 932, 964, 1097, 1129, 2587, 2619
AloI GAACNNNNNNTCC 2 cut(s) 1679, 1711
Alw21I GWGCWC 3 cut(s) 684, 776, 1086
Alw26I GTCTC 4 cut(s) 196, 286, 296, 1379
Alw44I GTGCAC 1 cut(s) 772
AlwI GGATC 6 cut(s) 65, 163, 355, 363, 1152, 1901
AlwNI CAGNNNCTG 1 cut(s) 1153
Ama87I CYCGRG 1 cut(s) 419
ApaLI GTGCAC 1 cut(s) 772
ApeKI GCWGC 2 cut(s) 2017, 2288
ApoI RAATTY 4 cut(s) 125, 667, 1375, 1792
Asp700I GAANNNNTTC 2 cut(s) 481, 2487
Asp718I GGTACC 1 cut(s) 909
AspLEI GCGC 2 cut(s) 333, 2417
AsuC2I CCSGG 2 cut(s) 628, 1469
AsuHPI GGTGA 6 cut(s) 340, 865, 967, 1649, 1696, 2168
AsuII TTCGAA 1 cut(s) 1232
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 6 cut(s) 382, 630, 831, 1052, 1118, 1471
AxyI CCTNAGG 1 cut(s) 23
BaeGI GKGCMC 4 cut(s) 411, 776, 1293, 1453
BanI GGYRCC 3 cut(s) 909, 1288, 1450
BanII GRGCYC 2 cut(s) 684, 2654
BarI GAAGNNNNNNTAC 2 cut(s) 1226, 1258
Bbv12I GWGCWC 3 cut(s) 684, 776, 1086
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 2 cut(s) 2004, 2275
BccI CCATC 6 cut(s) 567, 719, 1748, 2153, 2278, 2521
BceAI ACGGC 7 cut(s) 359, 451, 689, 805, 878, 904, 1009
BciT130I CCWGG 3 cut(s) 213, 352, 2115
BclI TGATCA 1 cut(s) 1576
BcnI CCSGG 2 cut(s) 628, 1469
BcoDI GTCTC 4 cut(s) 196, 286, 296, 1379
BfaI CTAG 1 cut(s) 2133
BfmI CTRYAG 1 cut(s) 2034
BfuAI ACCTGC 1 cut(s) 1513
BglI GCCNNNNNGGC 1 cut(s) 1003
BglII AGATCT 1 cut(s) 1012
BisI GCNGC 6 cut(s) 237, 240, 621, 2018, 2289, 2436
BlpI GCTNAGC 1 cut(s) 2238
BlsI GCNGC 6 cut(s) 238, 241, 622, 2019, 2290, 2437
BmcAI AGTACT 2 cut(s) 2192, 2390
Bme1390I CCNGG 5 cut(s) 213, 352, 628, 1469, 2115
Bme18I GGWCC 6 cut(s) 382, 630, 831, 1052, 1118, 1471
BmeT110I CYCGRG 1 cut(s) 419
BmrFI CCNGG 5 cut(s) 213, 352, 628, 1469, 2115
BmsI GCATC 7 cut(s) 880, 1117, 1156, 1159, 1753, 1836, 2402
BoxI GACNNNNGTC 1 cut(s) 1536
BplI GAGNNNNNCTC 2 cut(s) 2351, 2383
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 2 cut(s) 588, 2243
Bpu1102I GCTNAGC 1 cut(s) 2238
Bpu14I TTCGAA 1 cut(s) 1232
BpuEI CTTGAG 2 cut(s) 1398, 2642
BpuMI CCSGG 2 cut(s) 628, 1469
Bsa29I ATCGAT 3 cut(s) 158, 171, 603
BsaAI YACGTR 2 cut(s) 412, 2329
BsaBI GATNNNNATC 4 cut(s) 605, 1149, 1334, 1962
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 6 cut(s) 151, 211, 351, 1045, 1170, 1467
BsaWI WCCGGW 3 cut(s) 73, 1453, 1885
BsaXI ACNNNNNCTCC 4 cut(s) 684, 714, 2346, 2376
Bse118I RCCGGY 4 cut(s) 242, 269, 2059, 2344
Bse1I ACTGG 2 cut(s) 233, 1751
Bse21I CCTNAGG 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 1944
Bse8I GATNNNNATC 4 cut(s) 605, 1149, 1334, 1962
BseBI CCWGG 3 cut(s) 213, 352, 2115
BseCI ATCGAT 3 cut(s) 158, 171, 603
BseDI CCNNGG 6 cut(s) 151, 211, 351, 1045, 1170, 1467
BseGI GGATG 7 cut(s) 730, 895, 1579, 1768, 1777, 2208, 2289
BseJI GATNNNNATC 4 cut(s) 605, 1149, 1334, 1962
BseMI GCAATG 1 cut(s) 1944
BseMII CTCAG 5 cut(s) 579, 1779, 2208, 2229, 2682
BseNI ACTGG 2 cut(s) 233, 1751
BseRI GAGGAG 7 cut(s) 32, 35, 38, 41, 605, 898, 2372
BseSI GKGCMC 4 cut(s) 411, 776, 1293, 1453
BseXI GCAGC 2 cut(s) 2004, 2275
BseYI CCCAGC 2 cut(s) 1198, 2611
BsgI GTGCAG 1 cut(s) 1541
Bsh1236I CGCG 3 cut(s) 153, 710, 1328
BshNI GGYRCC 3 cut(s) 909, 1288, 1450
BshVI ATCGAT 3 cut(s) 158, 171, 603
BsiHKAI GWGCWC 3 cut(s) 684, 776, 1086
BsiHKCI CYCGRG 1 cut(s) 419
BslFI GGGAC 2 cut(s) 395, 643
BsmAI GTCTC 4 cut(s) 196, 286, 296, 1379
BsmBI CGTCTC 2 cut(s) 286, 296
BsmFI GGGAC 2 cut(s) 395, 643
BsmI GAATGC 2 cut(s) 1366, 2326
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 419
Bsp119I TTCGAA 1 cut(s) 1232
Bsp1286I GDGCHC 7 cut(s) 411, 684, 776, 1086, 1293, 1453, 2654
Bsp1407I TGTACA 4 cut(s) 780, 1242, 1332, 2071
Bsp1720I GCTNAGC 1 cut(s) 2238
Bsp19I CCATGG 1 cut(s) 1045
Bsp68I TCGCGA 1 cut(s) 1328
BspCNI CTCAG 5 cut(s) 580, 1780, 2207, 2230, 2681
BspDI ATCGAT 3 cut(s) 158, 171, 603
BspFNI CGCG 3 cut(s) 153, 710, 1328
BspMAI CTGCAG 1 cut(s) 2038
BspMI ACCTGC 1 cut(s) 1513
BspPI GGATC 6 cut(s) 65, 163, 355, 363, 1152, 1901
BspQI GCTCTTC 1 cut(s) 1640
BspT104I TTCGAA 1 cut(s) 1232
BspT107I GGYRCC 3 cut(s) 909, 1288, 1450
BspTNI GGTCTC 1 cut(s) 196
BsrBI CCGCTC 2 cut(s) 1180, 1650
BsrDI GCAATG 1 cut(s) 1944
BsrFI RCCGGY 4 cut(s) 242, 269, 2059, 2344
BsrGI TGTACA 4 cut(s) 780, 1242, 1332, 2071
BsrI ACTGG 2 cut(s) 233, 1751
BssAI RCCGGY 4 cut(s) 242, 269, 2059, 2344
BssECI CCNNGG 6 cut(s) 151, 211, 351, 1045, 1170, 1467
BssNAI GTATAC 3 cut(s) 1480, 1775, 2309
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 1045, 1170
Bst1107I GTATAC 3 cut(s) 1480, 1775, 2309
Bst2UI CCWGG 3 cut(s) 213, 352, 2115
Bst4CI ACNGT 6 cut(s) 767, 1112, 1562, 1863, 2127, 2561
Bst6I CTCTTC 2 cut(s) 1052, 1640
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 4 cut(s) 780, 1242, 1332, 2071
BstBAI YACGTR 2 cut(s) 412, 2329
BstBI TTCGAA 1 cut(s) 1232
BstC8I GCNNGC 2 cut(s) 244, 271
BstDEI CTNAG 9 cut(s) 23, 588, 1370, 1788, 1967, 2194, 2238, 2243, 2668
BstDSI CCRYGG 2 cut(s) 151, 1045
BstENI CCTNNNNNAGG 1 cut(s) 2371
BstF5I GGATG 7 cut(s) 730, 895, 1579, 1768, 1777, 2208, 2289
BstFNI CGCG 3 cut(s) 153, 710, 1328
BstHHI GCGC 2 cut(s) 333, 2417
BstMAI GTCTC 4 cut(s) 196, 286, 296, 1379
BstMWI GCNNNNNNNGC 4 cut(s) 529, 701, 1003, 2023
BstNI CCWGG 3 cut(s) 213, 352, 2115
BstNSI RCATGY 4 cut(s) 208, 1984, 2150, 2273
BstPAI GACNNNNGTC 1 cut(s) 1536
BstSCI CCNGG 5 cut(s) 211, 350, 626, 1467, 2113
BstSFI CTRYAG 1 cut(s) 2034
BstSLI GKGCMC 4 cut(s) 411, 776, 1293, 1453
BstSNI TACGTA 1 cut(s) 2329
BstUI CGCG 3 cut(s) 153, 710, 1328
BstV1I GCAGC 2 cut(s) 2004, 2275
BstX2I RGATCY 2 cut(s) 360, 1012
BstXI CCANNNNNNTGG 2 cut(s) 1046, 1435
BstYI RGATCY 2 cut(s) 360, 1012
BstZ17I GTATAC 3 cut(s) 1480, 1775, 2309
Bsu15I ATCGAT 3 cut(s) 158, 171, 603
Bsu36I CCTNAGG 1 cut(s) 23
BsuTUI ATCGAT 3 cut(s) 158, 171, 603
BtgI CCRYGG 2 cut(s) 151, 1045
BtgZI GCGATG 1 cut(s) 1343
BtsCI GGATG 7 cut(s) 730, 895, 1579, 1768, 1777, 2208, 2289
BtsIMutI CAGTG 3 cut(s) 226, 772, 1567
BtuMI TCGCGA 1 cut(s) 1328
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 2 cut(s) 244, 271
CaiI CAGNNNCTG 1 cut(s) 1153
CfoI GCGC 2 cut(s) 333, 2417
Cfr10I RCCGGY 4 cut(s) 242, 269, 2059, 2344
Cfr42I CCGCGG 1 cut(s) 154
ClaI ATCGAT 3 cut(s) 158, 171, 603
CseI GACGC 1 cut(s) 2486
DdeI CTNAG 9 cut(s) 23, 588, 1370, 1788, 1967, 2194, 2238, 2243, 2668
Eam1104I CTCTTC 2 cut(s) 1052, 1640
EarI CTCTTC 2 cut(s) 1052, 1640
Ecl136II GAGCTC 1 cut(s) 682
Eco105I TACGTA 1 cut(s) 2329
Eco130I CCWWGG 2 cut(s) 1045, 1170
Eco147I AGGCCT 1 cut(s) 2005
Eco24I GRGCYC 2 cut(s) 684, 2654
Eco31I GGTCTC 1 cut(s) 196
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 6 cut(s) 382, 630, 831, 1052, 1118, 1471
Eco53kI GAGCTC 1 cut(s) 682
Eco57I CTGAAG 2 cut(s) 1004, 1428
Eco81I CCTNAGG 1 cut(s) 23
Eco88I CYCGRG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 682
EcoNI CCTNNNNNAGG 1 cut(s) 2371
EcoO109I RGGNCCY 4 cut(s) 382, 1118, 1174, 1403
EcoRI GAATTC 3 cut(s) 125, 667, 1375
EcoRII CCWGG 3 cut(s) 211, 350, 2113
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 1045, 1170
EcoT22I ATGCAT 2 cut(s) 1986, 2326
EcoT38I GRGCYC 2 cut(s) 684, 2654
ErhI CCWWGG 2 cut(s) 1045, 1170
Esp3I CGTCTC 2 cut(s) 286, 296
FalI AAGNNNNNCTT 2 cut(s) 1750, 1782
FaqI GGGAC 2 cut(s) 395, 643
FauI CCCGC 5 cut(s) 703, 1185, 1227, 1356, 1725
FbaI TGATCA 1 cut(s) 1576
FblI GTMKAC 3 cut(s) 1479, 1774, 2308
Fnu4HI GCNGC 6 cut(s) 237, 240, 621, 2018, 2289, 2436
FokI GGATG 7 cut(s) 737, 902, 1586, 1764, 1775, 2195, 2296
FriOI GRGCYC 2 cut(s) 684, 2654
Fsp4HI GCNGC 6 cut(s) 237, 240, 621, 2018, 2289, 2436
FspBI CTAG 1 cut(s) 2133
GlaI GCGC 2 cut(s) 332, 2416
GluI GCNGC 6 cut(s) 237, 240, 621, 2018, 2289, 2436
GsaI CCCAGC 2 cut(s) 1202, 2615
GsuI CTGGAG 1 cut(s) 122
HgaI GACGC 1 cut(s) 2486
HhaI GCGC 2 cut(s) 333, 2417
Hin1I GRCGYC 1 cut(s) 222
Hin6I GCGC 2 cut(s) 331, 2415
HinP1I GCGC 2 cut(s) 331, 2415
HincII GTYRAC 2 cut(s) 112, 1531
HindII GTYRAC 2 cut(s) 112, 1531
HindIII AAGCTT 1 cut(s) 2171
HinfI GANTC 8 cut(s) 119, 160, 482, 595, 1963, 2377, 2633, 2678
HphI GGTGA 6 cut(s) 340, 865, 967, 1649, 1696, 2168
Hpy188III TCNNGA 5 cut(s) 1214, 1327, 2524, 2552, 2691
Hpy99I CGWCG 6 cut(s) 224, 281, 466, 472, 710, 1867
HpyAV CCTTC 9 cut(s) 193, 331, 465, 793, 821, 970, 1228, 1239, 1752
HpyCH4III ACNGT 6 cut(s) 767, 1112, 1562, 1863, 2127, 2561
HpyCH4IV ACGT 6 cut(s) 222, 305, 411, 578, 2328, 2687
HpyF10VI GCNNNNNNNGC 4 cut(s) 529, 701, 1003, 2023
HpyF3I CTNAG 9 cut(s) 23, 588, 1370, 1788, 1967, 2194, 2238, 2243, 2668
HpySE526I ACGT 6 cut(s) 222, 305, 411, 578, 2328, 2687
Hsp92I GRCGYC 1 cut(s) 222
HspAI GCGC 2 cut(s) 331, 2415
KpnI GGTACC 1 cut(s) 913
KroI GCCGGC 2 cut(s) 242, 269
KroNI GCCGGC 2 cut(s) 244, 271
Ksp22I TGATCA 1 cut(s) 1576
KspI CCGCGG 1 cut(s) 154
LguI GCTCTTC 1 cut(s) 1640
LmnI GCTCC 8 cut(s) 141, 153, 185, 213, 221, 457, 692, 1267
Lsp1109I GCAGC 2 cut(s) 2004, 2275
LweI GCATC 7 cut(s) 880, 1117, 1156, 1159, 1753, 1836, 2402
MaeI CTAG 1 cut(s) 2133
MaeII ACGT 6 cut(s) 222, 305, 411, 578, 2328, 2687
MaeIII GTNAC 4 cut(s) 346, 1186, 1585, 1990
MbiI CCGCTC 2 cut(s) 1180, 1650
MfeI CAATTG 1 cut(s) 2602
MflI RGATCY 2 cut(s) 360, 1012
MhlI GDGCHC 7 cut(s) 411, 684, 776, 1086, 1293, 1453, 2654
MlyI GAGTC 1 cut(s) 2627
MmeI TCCRAC 6 cut(s) 243, 423, 1368, 1493, 1688, 1805
Mph1103I ATGCAT 2 cut(s) 1986, 2326
MroNI GCCGGC 2 cut(s) 242, 269
MroXI GAANNNNTTC 2 cut(s) 481, 2487
MseI TTAA 4 cut(s) 1248, 1923, 2151, 2274
MspA1I CMGCKG 2 cut(s) 153, 2448
MspR9I CCNGG 5 cut(s) 213, 352, 628, 1469, 2115
MunI CAATTG 1 cut(s) 2602
Mva1269I GAATGC 2 cut(s) 1366, 2326
MvaI CCWGG 3 cut(s) 213, 352, 2115
MvnI CGCG 3 cut(s) 153, 710, 1328
MwoI GCNNNNNNNGC 4 cut(s) 529, 701, 1003, 2023
NaeI GCCGGC 2 cut(s) 244, 271
NciI CCSGG 2 cut(s) 628, 1469
NcoI CCATGG 1 cut(s) 1045
NgoMIV GCCGGC 2 cut(s) 242, 269
NmuCI GTSAC 1 cut(s) 346
NruI TCGCGA 1 cut(s) 1328
NsiI ATGCAT 2 cut(s) 1986, 2326
NspI RCATGY 4 cut(s) 208, 1984, 2150, 2273
NspV TTCGAA 1 cut(s) 1232
PaqCI CACCTGC 1 cut(s) 1513
PceI AGGCCT 1 cut(s) 2005
PciI ACATGT 2 cut(s) 2146, 2269
PciSI GCTCTTC 1 cut(s) 1640
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 2 cut(s) 1366, 2326
PdiI GCCGGC 2 cut(s) 244, 271
PdmI GAANNNNTTC 2 cut(s) 481, 2487
PfeI GAWTC 7 cut(s) 119, 160, 482, 595, 1963, 2377, 2678
PflFI GACNNNGTC 2 cut(s) 344, 1603
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 6 cut(s) 238, 241, 622, 2019, 2290, 2437
PleI GAGTC 1 cut(s) 2627
PpsI GAGTC 1 cut(s) 2627
Ppu21I YACGTR 2 cut(s) 412, 2329
PpuMI RGGWCCY 2 cut(s) 382, 1118
PscI ACATGT 2 cut(s) 2146, 2269
PshAI GACNNNNGTC 1 cut(s) 1536
Psp124BI GAGCTC 1 cut(s) 684
Psp5II RGGWCCY 2 cut(s) 382, 1118
Psp6I CCWGG 3 cut(s) 211, 350, 2113
PspFI CCCAGC 2 cut(s) 1198, 2611
PspGI CCWGG 3 cut(s) 211, 350, 2113
PspPPI RGGWCCY 2 cut(s) 382, 1118
PsrI GAACNNNNNNTAC 2 cut(s) 734, 766
PstI CTGCAG 1 cut(s) 2038
PstNI CAGNNNCTG 1 cut(s) 1153
PsuI RGATCY 2 cut(s) 360, 1012
PsyI GACNNNGTC 2 cut(s) 344, 1603
RruI TCGCGA 1 cut(s) 1328
SacI GAGCTC 1 cut(s) 684
SacII CCGCGG 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 1640
SaqAI TTAA 4 cut(s) 1248, 1923, 2151, 2274
SatI GCNGC 6 cut(s) 237, 240, 621, 2018, 2289, 2436
ScaI AGTACT 2 cut(s) 2192, 2390
SchI GAGTC 1 cut(s) 2627
ScrFI CCNGG 5 cut(s) 213, 352, 628, 1469, 2115
SduI GDGCHC 7 cut(s) 411, 684, 776, 1086, 1293, 1453, 2654
SfaNI GCATC 7 cut(s) 880, 1117, 1156, 1159, 1753, 1836, 2402
SfcI CTRYAG 1 cut(s) 2034
SfiI GGCCNNNNNGGCC 1 cut(s) 1003
Sfr303I CCGCGG 1 cut(s) 154
SfuI TTCGAA 1 cut(s) 1232
SgrBI CCGCGG 1 cut(s) 154
SinI GGWCC 6 cut(s) 382, 630, 831, 1052, 1118, 1471
SmlI CTYRAG 2 cut(s) 1413, 2621
SmoI CTYRAG 2 cut(s) 1413, 2621
SnaBI TACGTA 1 cut(s) 2329
SseBI AGGCCT 1 cut(s) 2005
SspI AATATT 1 cut(s) 1720
SspMI CTAG 1 cut(s) 2133
SstI GAGCTC 1 cut(s) 684
StuI AGGCCT 1 cut(s) 2005
StyD4I CCNGG 5 cut(s) 211, 350, 626, 1467, 2113
StyI CCWWGG 2 cut(s) 1045, 1170
TaaI ACNGT 6 cut(s) 767, 1112, 1562, 1863, 2127, 2561
TaiI ACGT 6 cut(s) 225, 308, 414, 581, 2331, 2690
TaqII GACCGA 2 cut(s) 1061, 1069
TatI WGTACW 7 cut(s) 762, 780, 1242, 1332, 2071, 2190, 2388
TauI GCSGC 4 cut(s) 239, 242, 623, 2438
TfiI GAWTC 7 cut(s) 119, 160, 482, 595, 1963, 2377, 2678
Tru1I TTAA 4 cut(s) 1248, 1923, 2151, 2274
Tru9I TTAA 4 cut(s) 1248, 1923, 2151, 2274
TscAI CASTG 3 cut(s) 233, 772, 1567
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 2 cut(s) 2017, 2288
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 7 cut(s) 503, 650, 989, 1149, 2165, 2384, 2468
TspGWI ACGGA 5 cut(s) 25, 507, 980, 1548, 2673
TspRI CASTG 3 cut(s) 233, 772, 1567
Tth111I GACNNNGTC 2 cut(s) 344, 1603
Van91I CCANNNNNTGG 1 cut(s) 845
VneI GTGCAC 1 cut(s) 772
VpaK11BI GGWCC 6 cut(s) 382, 630, 831, 1052, 1118, 1471
XagI CCTNNNNNAGG 1 cut(s) 2371
XapI RAATTY 4 cut(s) 125, 667, 1375, 1792
XceI RCATGY 4 cut(s) 208, 1984, 2150, 2273
XcmI CCANNNNNNNNNTGG 2 cut(s) 640, 1046
XmiI GTMKAC 3 cut(s) 1479, 1774, 2308
XmnI GAANNNNTTC 2 cut(s) 481, 2487
XspI CTAG 1 cut(s) 2133
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 2 cut(s) 2192, 2390
Zsp2I ATGCAT 2 cut(s) 1986, 2326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.