Rh6DG403400

Electron transfer DM13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0014477
Physical Location & Seq
Forward (+)
59163957 .. 59167266
3310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG403400.1

Sequence Viewer

Length: 2544 bp
ATGCCTCGCAAGCCCACCGCCACCATCTTCCTAGGGTTTCTCCTCCTCCTCCTCCTCCTTACATTCTGCCATGCCGATCCGGGTTCCAATTGCCCCAAAACCAGCCCTCTCGTCAACTTGGAATCGCAATTCAAAATGCTCCAGCACCAGCTCAGAGGCTCCGTTAAGATAATCGACGATTGCTCCTTCCAGGTTTCCAATTTCGACATGCTCTCCGGCTCCGACGTCCACTGGTGGGGCGCCGTCGCCCCCGATTTCAATAATCTCACCGCCGGCTTCGTAATCTCCGACCAGAAGCTCAACGAGACATACAAAAGCGCGAGCTTCACTGTGCGCCTGAGGGACAATGTCACCTGGGATCAGATCCAAGTCCTCGCCGTCTGGGACCTCCCCACCTCCTCCGACTTCGGTCACATCGTGCTCCGGGATGTAGTAAACAGATCGTCCGGCAGCTCCGATTTGGCTCCTTCTCCGTCGCCGGCGAGCGATTCAGGGAATGGGACCGGTCAGGTTCACACTGAGCCGACTATGTTGGTGAATTGCAAGAGTTTGTCCGACGATTTTAGGGTTAGATGGACACTGCGTCCTGAGGAGAATGTGATTGACATTGGGCTGGAGGCTGCCACCGGGTCGACGAATTACATGGCTTTCGGGTGGGCCCGCCCGAATATCACACACCAGTTTATGCTCGGAGCTGACGTGGCCGTCGCCGGATTCAAGGAGGACGGAATGCCGTTTGTGGATGATTTTTACATCACAAAGTACAGCGAGTGCACCGTGTACAAGGACGGCTCCGTCAAAGGGGTGTGCCCCGATACAAGATACGAAGGGACGGCCCCGAATGGCTTGGTGAACAATACCAAATTGGTTTACGGCCACCGGAGGGACGCCGTGTCGTTTATTAGGTACCAGAGGCCGTTGGAATCAGCTGATAAAAAGTATGATTTGGCGGTGAAGCATACCGAGAAAAGGACGGTGATTTGGGCCGTGGGGCCGATCAGGCCACCGGATACTCTTCAGCCTTACTATCTTCCCCAAAACCATGGGGGACCCCAGGATGTGGTTTATGGCCATTTGGAGCTCAATGTGTCTGAGCATGTGGATGACTGTTATGGTCCTATAGAGGCCGATGACAAGGAGGATCAGCACCTCATTATTGCGGATGCAAAGGCCCCGCTCGTGGTTACTTCCGGGCAGGCAGTGCATTATCCAGACCCTCCGAACCCTTCAAAGGTTCTTTACATTAACAAGAAAGAGGCTCCGGTGTTGAGAGTCGAAAGAGGGGTGCCGGTCACGTTTTCAATACAAGCTGGGCATGATGTTGCTCTCTACATTACTTCAGATCCTCTTGGTGGGAATGCTACACTGAGGAATGCAAGCGAGACTGTGTATGCTGGAGGATCGGAAGCTCAAGGAGTTCAAGCCAGTCCCAAGGAGTTAATTTGGGCACCAGACAGGAATACCCCAGACCTAGTGTACTATCAATCTCTTTATGACCAGAAAATGGGATGGAAGATACAGGTGGTTGATGGGGGTCTGTCAGATATGTATAATAACAGTGTGATTTTGGATGATCAGCAAGTTACCTTATTTTGGACACTGGCACATGATTCTATATCTATTGCAGTCCGGGGTGAGAAGAAAAGTGGTTTCGTGGCAATAGGATTTGGTAGGGGAATGGTGAACAGCTATGCTTATGTGGGTTGGATTGATAATATTGGCAAAGGGCGGGTAAATACTTACTGGATTGATGGAAAGGATGCCTCGAGTGTGCATCCAACACATGAGAATTTGACATATGTGAGGTGCAGGTCAGAAAATGGGATCATTACGTTCGAGTTCACTCGTCCTTTGAAACCATCATGTGGTAAGAGTGATAAGCCCGAGTGTAAAAACATAATTGATCCAACTACTCCTCTTAAAATTATATGGGCAATGGGTGCTACATGGACCGATGATCATCTTAGTGATCAAAACATGCATTTTGCCACCAGCAGTAGGCCTATAAGGGTGCTGCTTATGCGTGGTTCTGCAGAGGCAGAGCAGGATTTACAGCCAGTATTAGCAGTGCATGGATTTATGATGTTTCTCGCTTGGGCTATCTTGCTTCCTGGTGGAATACTGGCGGCTAGATACTTAAAACATGTTAAGGGTGATGGTTGGTACCGGATTCATGTATACTTGCAGTACTCAGGTTTGGTAATCGTTCTACTTGCCCTTCTTTTTGCCGTTGCTGAGCTCCGTGGTTTCTTTTTCGGCTCATTACATGTTAAATTTGGAACTACGGCTATACTTTTGGTTTGTATGCAACCAGTGAATGCATACCTAAGGCCAAAGAGACCAAATAATGGAGAGCTGGTTTCCTCTAAAAGGCTTCTATGGGAGTATCTTCATGTGATCGGGGGCAGATCTGCCATTGTAGTAGGTTTTGGAGCACTTTTCACAGGACTGAAGCATTTAGGCAAAGAAGGAGGAATAGAAGTATCGGCAGAAGCAATTGGGTGCTGGGGAACCTTGAGGAGGATGACTCTGTTGATTTGTTGA

Protein Analysis

847

Amino Acids

93.68

Weight (kDa)

6.28

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DM13 PF10517 43 - 140 3.2e-15 Electron transfer DM13
DOMON PF03351 186 - 331 8.9e-12 DOMON domain
At5g54830 PF25489 385 - 510 1.1e-69 At5g54830-like domain
DOMON PF03351 524 - 647 3.6e-25 DOMON domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54830
malus_domestica MD08G1058600.v1.1 MD15G1037900.v1.1
prunus_persica Prupe.1G391800_v2.0.a1
pyrus_communis pycom08g04850 pycom15g03510
rosa_chinensis RchiOBHm_Chr6g0299641
rosa_laevigata RLG00000011400
rosa_multiflora Rmu_co8233929.1_g000001 Rmu_co8458799.1_g000001 Rmu_sc0002895.1_g000005
rosa_roxburghii Rroxscaffold_7G00168480
rosa_rugosa Rorug06G0291300
rosa_samantha Rh6BG410500 Rh6CG416200 Rh6DG403400
rosa_wichuraiana Rw6G035190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 704, 794
AatII GACGTC 1 cut(s) 228
Acc36I ACCTGC 1 cut(s) 1800
Acc65I GGTACC 2 cut(s) 906, 2163
AccB1I GGYRCC 5 cut(s) 239, 906, 1285, 1447, 2163
AccB7I CCANNNNNTGG 4 cut(s) 1060, 1504, 1865, 2348
AccBSI CCGCTC 1 cut(s) 1177
AccI GTMKAC 2 cut(s) 632, 2178
AccII CGCG 1 cut(s) 320
AciI CCGC 8 cut(s) 18, 270, 661, 950, 1160, 1175, 1729, 2126
AclWI GGATC 8 cut(s) 71, 358, 366, 1149, 1337, 1408, 1832, 1898
AcoI YGGCCR 3 cut(s) 702, 874, 1069
AcsI RAATTY 2 cut(s) 1789, 2273
AcuI CTGAAG 3 cut(s) 1001, 1323, 2471
AcyI GRCGYC 3 cut(s) 225, 240, 888
AdeI CACNNNGTG 1 cut(s) 418
AfaI GTAC 6 cut(s) 764, 782, 908, 1478, 2165, 2189
AflIII ACRYGT 2 cut(s) 2143, 2266
AgeI ACCGGT 1 cut(s) 503
AgsI TTSAA 7 cut(s) 133, 259, 718, 1230, 1302, 1421, 1855
AhdI GACNNNNNGTC 2 cut(s) 582, 892
AjiI CACGTC 1 cut(s) 700
AjnI CCWGG 4 cut(s) 189, 353, 1053, 2110
Alw21I GWGCWC 5 cut(s) 423, 776, 1083, 2241, 2437
Alw26I GTCTC 3 cut(s) 299, 1376, 2332
Alw44I GTGCAC 1 cut(s) 772
AlwI GGATC 8 cut(s) 71, 358, 366, 1149, 1337, 1408, 1832, 1898
Ama87I CYCGRG 2 cut(s) 1765, 1883
ApaI GGGCCC 1 cut(s) 661
ApaLI GTGCAC 1 cut(s) 772
ApeKI GCWGC 3 cut(s) 450, 620, 2014
ApoI RAATTY 2 cut(s) 1789, 2273
AsiGI ACCGGT 1 cut(s) 503
Asp718I GGTACC 2 cut(s) 906, 2163
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 3 cut(s) 242, 320, 336
AsuC2I CCSGG 5 cut(s) 81, 425, 628, 1192, 1631
AsuHPI GGTGA 9 cut(s) 259, 343, 547, 862, 964, 988, 1646, 1693, 2165
AvaI CYCGRG 2 cut(s) 1765, 1883
AvaII GGWCC 5 cut(s) 385, 501, 1049, 1115, 1950
AvrII CCTAGG 1 cut(s) 31
AxyI CCTNAGG 3 cut(s) 338, 588, 2327
BaeGI GKGCMC 4 cut(s) 661, 776, 812, 1450
BalI TGGCCA 1 cut(s) 1071
BanI GGYRCC 5 cut(s) 239, 906, 1285, 1447, 2163
BanII GRGCYC 3 cut(s) 661, 1083, 2241
BauI CACGAG 1 cut(s) 1178
Bbv12I GWGCWC 5 cut(s) 423, 776, 1083, 2241, 2437
BbvI GCAGC 3 cut(s) 462, 607, 2001
BccI CCATC 7 cut(s) 32, 567, 1503, 1523, 1745, 1867, 2150
BciT130I CCWGG 4 cut(s) 191, 355, 1055, 2112
BciVI GTATCC 1 cut(s) 1003
BclI TGATCA 3 cut(s) 1573, 1957, 1969
BcnI CCSGG 5 cut(s) 81, 425, 628, 1192, 1631
BcoDI GTCTC 3 cut(s) 299, 1376, 2332
BfaI CTAG 3 cut(s) 32, 1472, 2130
BfmI CTRYAG 2 cut(s) 1119, 2031
BfoI RGCGCY 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 1800
BfuI GTATCC 1 cut(s) 1003
BglI GCCNNNNNGGC 1 cut(s) 1000
BglII AGATCT 1 cut(s) 2408
BisI GCNGC 4 cut(s) 451, 621, 2015, 2127
BlnI CCTAGG 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 2235
BlsI GCNGC 4 cut(s) 452, 622, 2016, 2128
BmcAI AGTACT 1 cut(s) 2189
Bme1390I CCNGG 9 cut(s) 81, 191, 355, 425, 628, 1055, 1192, 1631, 2112
Bme18I GGWCC 5 cut(s) 385, 501, 1049, 1115, 1950
BmeRI GACNNNNNGTC 2 cut(s) 582, 892
BmeT110I CYCGRG 2 cut(s) 1765, 1883
BmgBI CACGTC 1 cut(s) 700
BmrFI CCNGG 9 cut(s) 81, 191, 355, 425, 628, 1055, 1192, 1631, 2112
BmsI GCATC 3 cut(s) 1153, 1750, 1783
BoxI GACNNNNGTC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 2512, 2544
BpmI CTGGAG 3 cut(s) 125, 635, 1416
Bpu1102I GCTNAGC 1 cut(s) 2235
BpuEI CTTGAG 2 cut(s) 1395, 2536
BpuMI CCSGG 5 cut(s) 81, 425, 628, 1192, 1631
BsaBI GATNNNNATC 1 cut(s) 1959
BsaHI GRCGYC 3 cut(s) 225, 240, 888
BsaI GGTCTC 1 cut(s) 2332
BsaJI CCNNGG 8 cut(s) 31, 354, 987, 1042, 1053, 1431, 1630, 2242
BsaWI WCCGGW 5 cut(s) 503, 879, 1006, 1261, 2166
BsaXI ACNNNNNCTCC 8 cut(s) 167, 197, 197, 227, 684, 714, 2343, 2373
Bse118I RCCGGY 4 cut(s) 272, 478, 503, 1288
Bse1I ACTGG 8 cut(s) 236, 679, 1425, 1605, 1748, 2057, 2127, 2312
Bse21I CCTNAGG 3 cut(s) 338, 588, 2327
Bse3DI GCAATG 1 cut(s) 1941
Bse8I GATNNNNATC 1 cut(s) 1959
BseBI CCWGG 4 cut(s) 191, 355, 1055, 2112
BseDI CCNNGG 8 cut(s) 31, 354, 987, 1042, 1053, 1431, 1630, 2242
BseJI GATNNNNATC 1 cut(s) 1959
BseMI GCAATG 1 cut(s) 1941
BseMII CTCAG 8 cut(s) 166, 329, 510, 579, 1083, 1358, 2205, 2226
BseNI ACTGG 8 cut(s) 236, 679, 1425, 1605, 1748, 2057, 2127, 2312
BseRI GAGGAG 9 cut(s) 32, 35, 38, 41, 44, 388, 605, 1905, 2533
BseSI GKGCMC 4 cut(s) 661, 776, 812, 1450
BseXI GCAGC 3 cut(s) 462, 607, 2001
BseYI CCCAGC 2 cut(s) 1310, 2505
BsgI GTGCAG 1 cut(s) 1828
Bsh1236I CGCG 1 cut(s) 320
BshNI GGYRCC 5 cut(s) 239, 906, 1285, 1447, 2163
BshTI ACCGGT 1 cut(s) 503
BsiHKAI GWGCWC 5 cut(s) 423, 776, 1083, 2241, 2437
BsiHKCI CYCGRG 2 cut(s) 1765, 1883
BslFI GGGAC 7 cut(s) 356, 398, 514, 844, 899, 1062, 1413
BsmAI GTCTC 3 cut(s) 299, 1376, 2332
BsmFI GGGAC 7 cut(s) 356, 398, 514, 844, 899, 1062, 1413
BsmI GAATGC 4 cut(s) 735, 1363, 1378, 2323
Bso31I GGTCTC 1 cut(s) 2332
BsoBI CYCGRG 2 cut(s) 1765, 1883
Bsp120I GGGCCC 1 cut(s) 657
Bsp1286I GDGCHC 8 cut(s) 423, 661, 776, 812, 1083, 1450, 2241, 2437
Bsp1407I TGTACA 1 cut(s) 780
Bsp1720I GCTNAGC 1 cut(s) 2235
Bsp19I CCATGG 1 cut(s) 1042
BspACI CCGC 8 cut(s) 18, 270, 661, 950, 1160, 1175, 1729, 2126
BspCNI CTCAG 8 cut(s) 165, 330, 511, 580, 1084, 1359, 2204, 2227
BspFNI CGCG 1 cut(s) 320
BspMAI CTGCAG 1 cut(s) 2035
BspMI ACCTGC 1 cut(s) 1800
BspPI GGATC 8 cut(s) 71, 358, 366, 1149, 1337, 1408, 1832, 1898
BspT107I GGYRCC 5 cut(s) 239, 906, 1285, 1447, 2163
BspTNI GGTCTC 1 cut(s) 2332
BsrBI CCGCTC 1 cut(s) 1177
BsrDI GCAATG 1 cut(s) 1941
BsrFI RCCGGY 4 cut(s) 272, 478, 503, 1288
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 8 cut(s) 236, 679, 1425, 1605, 1748, 2057, 2127, 2312
BssAI RCCGGY 4 cut(s) 272, 478, 503, 1288
BssECI CCNNGG 8 cut(s) 31, 354, 987, 1042, 1053, 1431, 1630, 2242
BssNAI GTATAC 1 cut(s) 2179
BssNI GRCGYC 3 cut(s) 225, 240, 888
BssSI CACGAG 1 cut(s) 1178
BssT1I CCWWGG 3 cut(s) 31, 1042, 1431
Bst1107I GTATAC 1 cut(s) 2179
Bst2BI CACGAG 1 cut(s) 1178
Bst2UI CCWGG 4 cut(s) 191, 355, 1055, 2112
Bst4CI ACNGT 6 cut(s) 331, 778, 976, 1109, 1387, 1559
Bst6I CTCTTC 1 cut(s) 1020
BstACI GRCGYC 3 cut(s) 225, 240, 888
BstAPI GCANNNNNTGC 2 cut(s) 1201, 1940
BstAUI TGTACA 1 cut(s) 780
BstC8I GCNNGC 8 cut(s) 11, 274, 322, 480, 484, 661, 1197, 1378
BstDSI CCRYGG 3 cut(s) 987, 1042, 2242
BstENI CCTNNNNNAGG 2 cut(s) 2368, 2518
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 3 cut(s) 242, 320, 336
BstMAI GTCTC 3 cut(s) 299, 1376, 2332
BstMWI GCNNNNNNNGC 6 cut(s) 10, 701, 1000, 1201, 1940, 2020
BstNI CCWGG 4 cut(s) 191, 355, 1055, 2112
BstNSI RCATGY 5 cut(s) 211, 1100, 1981, 2147, 2270
BstPAI GACNNNNGTC 1 cut(s) 408
BstSCI CCNGG 9 cut(s) 79, 189, 353, 423, 626, 1053, 1190, 1629, 2110
BstSFI CTRYAG 2 cut(s) 1119, 2031
BstSLI GKGCMC 4 cut(s) 661, 776, 812, 1450
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 3 cut(s) 462, 607, 2001
BstX2I RGATCY 3 cut(s) 363, 1342, 2408
BstXI CCANNNNNNTGG 1 cut(s) 1043
BstYI RGATCY 3 cut(s) 363, 1342, 2408
BstZ17I GTATAC 1 cut(s) 2179
Bsu36I CCTNAGG 3 cut(s) 338, 588, 2327
BsuI GTATCC 1 cut(s) 1003
BtgI CCRYGG 3 cut(s) 987, 1042, 2242
BtrI CACGTC 1 cut(s) 700
BtsI GCAGTG 3 cut(s) 578, 1206, 2073
BveI ACCTGC 1 cut(s) 1800
Cac8I GCNNGC 8 cut(s) 11, 274, 322, 480, 484, 661, 1197, 1378
CfoI GCGC 3 cut(s) 242, 320, 336
Cfr10I RCCGGY 4 cut(s) 272, 478, 503, 1288
CseI GACGC 2 cut(s) 572, 896
Csp6I GTAC 6 cut(s) 763, 781, 907, 1477, 2164, 2188
CspAI ACCGGT 1 cut(s) 503
CviQI GTAC 6 cut(s) 763, 781, 907, 1477, 2164, 2188
DinI GGCGCC 1 cut(s) 241
DraIII CACNNNGTG 1 cut(s) 418
DrdI GACNNNNNNGTC 2 cut(s) 704, 794
DriI GACNNNNNGTC 2 cut(s) 582, 892
DseDI GACNNNNNNGTC 2 cut(s) 704, 794
EaeI YGGCCR 3 cut(s) 702, 874, 1069
Eam1104I CTCTTC 1 cut(s) 1020
Eam1105I GACNNNNNGTC 2 cut(s) 582, 892
EarI CTCTTC 1 cut(s) 1020
Ecl136II GAGCTC 2 cut(s) 1081, 2239
Eco130I CCWWGG 3 cut(s) 31, 1042, 1431
Eco147I AGGCCT 1 cut(s) 2002
Eco24I GRGCYC 3 cut(s) 661, 1083, 2241
Eco31I GGTCTC 1 cut(s) 2332
Eco47I GGWCC 5 cut(s) 385, 501, 1049, 1115, 1950
Eco53kI GAGCTC 2 cut(s) 1081, 2239
Eco57I CTGAAG 3 cut(s) 1001, 1323, 2471
Eco81I CCTNAGG 3 cut(s) 338, 588, 2327
Eco88I CYCGRG 2 cut(s) 1765, 1883
EcoICRI GAGCTC 2 cut(s) 1081, 2239
EcoNI CCTNNNNNAGG 2 cut(s) 2368, 2518
EcoO109I RGGNCCY 3 cut(s) 385, 1049, 1171
EcoRII CCWGG 4 cut(s) 189, 353, 1053, 2110
EcoT14I CCWWGG 3 cut(s) 31, 1042, 1431
EcoT22I ATGCAT 2 cut(s) 1983, 2323
EcoT38I GRGCYC 3 cut(s) 661, 1083, 2241
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
ErhI CCWWGG 3 cut(s) 31, 1042, 1431
FaqI GGGAC 7 cut(s) 356, 398, 514, 844, 899, 1062, 1413
FauI CCCGC 3 cut(s) 668, 1182, 1722
FauNDI CATATG 1 cut(s) 1798
FbaI TGATCA 3 cut(s) 1573, 1957, 1969
FblI GTMKAC 2 cut(s) 632, 2178
Fnu4HI GCNGC 4 cut(s) 451, 621, 2015, 2127
FriOI GRGCYC 3 cut(s) 661, 1083, 2241
Fsp4HI GCNGC 4 cut(s) 451, 621, 2015, 2127
FspBI CTAG 3 cut(s) 32, 1472, 2130
GlaI GCGC 3 cut(s) 241, 319, 335
GluI GCNGC 4 cut(s) 451, 621, 2015, 2127
GsaI CCCAGC 2 cut(s) 1314, 2509
GsuI CTGGAG 3 cut(s) 125, 635, 1416
HaeII RGCGCY 1 cut(s) 243
HgaI GACGC 2 cut(s) 572, 896
HhaI GCGC 3 cut(s) 242, 320, 336
Hin1I GRCGYC 3 cut(s) 225, 240, 888
Hin6I GCGC 3 cut(s) 240, 318, 334
HinP1I GCGC 3 cut(s) 240, 318, 334
HincII GTYRAC 2 cut(s) 115, 633
HindII GTYRAC 2 cut(s) 115, 633
HinfI GANTC 8 cut(s) 122, 488, 714, 923, 1272, 1610, 2170, 2527
HphI GGTGA 9 cut(s) 259, 343, 547, 862, 964, 988, 1646, 1693, 2165
Hpy188III TCNNGA 2 cut(s) 587, 1211
Hpy99I CGWCG 7 cut(s) 179, 227, 248, 478, 560, 637, 710
HpyAV CCTTC 6 cut(s) 196, 477, 821, 1236, 2228, 2462
HpyCH4III ACNGT 6 cut(s) 331, 778, 976, 1109, 1387, 1559
HpyCH4IV ACGT 4 cut(s) 225, 699, 1295, 1832
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 701, 1000, 1201, 1940, 2020
HpySE526I ACGT 4 cut(s) 225, 699, 1295, 1832
Hsp92I GRCGYC 3 cut(s) 225, 240, 888
HspAI GCGC 3 cut(s) 240, 318, 334
KasI GGCGCC 1 cut(s) 239
KflI GGGWCCC 1 cut(s) 1049
KpnI GGTACC 2 cut(s) 910, 2167
KroI GCCGGC 2 cut(s) 272, 478
KroNI GCCGGC 2 cut(s) 274, 480
Ksp22I TGATCA 3 cut(s) 1573, 1957, 1969
Lsp1109I GCAGC 3 cut(s) 462, 607, 2001
LweI GCATC 3 cut(s) 1153, 1750, 1783
MaeI CTAG 3 cut(s) 32, 1472, 2130
MaeII ACGT 4 cut(s) 225, 699, 1295, 1832
MaeIII GTNAC 5 cut(s) 349, 410, 1183, 1291, 1582
MbiI CCGCTC 1 cut(s) 1177
MboII GAAGA 6 cut(s) 19, 1007, 1022, 1525, 1651, 2381
MfeI CAATTG 2 cut(s) 88, 2496
MflI RGATCY 3 cut(s) 363, 1342, 2408
MhlI GDGCHC 8 cut(s) 423, 661, 776, 812, 1083, 1450, 2241, 2437
MlsI TGGCCA 1 cut(s) 1071
MluNI TGGCCA 1 cut(s) 1071
Mly113I GGCGCC 1 cut(s) 240
MlyI GAGTC 2 cut(s) 1281, 2521
MmeI TCCRAC 8 cut(s) 246, 312, 426, 579, 900, 1685, 1802, 1931
Mox20I TGGCCA 1 cut(s) 1071
Mph1103I ATGCAT 2 cut(s) 1983, 2323
MreI CGCCGGCG 1 cut(s) 478
MroNI GCCGGC 2 cut(s) 272, 478
MscI TGGCCA 1 cut(s) 1071
MseI TTAA 7 cut(s) 165, 1245, 1439, 1920, 2138, 2148, 2271
MslI CAYNNNNRTG 2 cut(s) 1101, 1965
Msp20I TGGCCA 1 cut(s) 1071
MspA1I CMGCKG 1 cut(s) 929
MspR9I CCNGG 9 cut(s) 81, 191, 355, 425, 628, 1055, 1192, 1631, 2112
MunI CAATTG 2 cut(s) 88, 2496
Mva1269I GAATGC 4 cut(s) 735, 1363, 1378, 2323
MvaI CCWGG 4 cut(s) 191, 355, 1055, 2112
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 6 cut(s) 10, 701, 1000, 1201, 1940, 2020
NaeI GCCGGC 2 cut(s) 274, 480
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 5 cut(s) 81, 425, 628, 1192, 1631
NcoI CCATGG 1 cut(s) 1042
NdeI CATATG 1 cut(s) 1798
NgoMIV GCCGGC 2 cut(s) 272, 478
NmuCI GTSAC 3 cut(s) 349, 410, 1291
NsiI ATGCAT 2 cut(s) 1983, 2323
NspI RCATGY 5 cut(s) 211, 1100, 1981, 2147, 2270
PaeR7I CTCGAG 1 cut(s) 1765
PceI AGGCCT 1 cut(s) 2002
PciI ACATGT 2 cut(s) 2143, 2266
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 414, 696
PctI GAATGC 4 cut(s) 735, 1363, 1378, 2323
PdiI GCCGGC 2 cut(s) 274, 480
PfeI GAWTC 6 cut(s) 122, 488, 714, 923, 1610, 2170
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 4 cut(s) 1060, 1504, 1865, 2348
PfoI TCCNGGA 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 503
PkrI GCNGC 4 cut(s) 452, 622, 2016, 2128
PleI GAGTC 2 cut(s) 1280, 2521
PluTI GGCGCC 1 cut(s) 243
PpsI GAGTC 2 cut(s) 1280, 2521
PpuMI RGGWCCY 2 cut(s) 385, 1049
PscI ACATGT 2 cut(s) 2143, 2266
PshAI GACNNNNGTC 1 cut(s) 408
Psp124BI GAGCTC 2 cut(s) 1083, 2241
Psp5II RGGWCCY 2 cut(s) 385, 1049
Psp6I CCWGG 4 cut(s) 189, 353, 1053, 2110
PspFI CCCAGC 2 cut(s) 1310, 2505
PspGI CCWGG 4 cut(s) 189, 353, 1053, 2110
PspOMI GGGCCC 1 cut(s) 657
PspPPI RGGWCCY 2 cut(s) 385, 1049
PspXI VCTCGAGB 1 cut(s) 1765
PstI CTGCAG 1 cut(s) 2035
PsuI RGATCY 3 cut(s) 363, 1342, 2408
PsyI GACNNNGTC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 929
RsaI GTAC 6 cut(s) 764, 782, 908, 1478, 2165, 2189
RsaNI GTAC 6 cut(s) 763, 781, 907, 1477, 2164, 2188
RseI CAYNNNNRTG 2 cut(s) 1101, 1965
SacI GAGCTC 2 cut(s) 1083, 2241
SalI GTCGAC 1 cut(s) 631
SaqAI TTAA 7 cut(s) 165, 1245, 1439, 1920, 2138, 2148, 2271
SatI GCNGC 4 cut(s) 451, 621, 2015, 2127
ScaI AGTACT 1 cut(s) 2189
SchI GAGTC 2 cut(s) 1281, 2521
ScrFI CCNGG 9 cut(s) 81, 191, 355, 425, 628, 1055, 1192, 1631, 2112
SduI GDGCHC 8 cut(s) 423, 661, 776, 812, 1083, 1450, 2241, 2437
SfaNI GCATC 3 cut(s) 1153, 1750, 1783
SfcI CTRYAG 2 cut(s) 1119, 2031
SfiI GGCCNNNNNGGCC 1 cut(s) 1000
SfoI GGCGCC 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 1765
SgrAI CRCCGGYG 1 cut(s) 478
SinI GGWCC 5 cut(s) 385, 501, 1049, 1115, 1950
SlaI CTCGAG 1 cut(s) 1765
SmiMI CAYNNNNRTG 2 cut(s) 1101, 1965
SmlI CTYRAG 3 cut(s) 1410, 1765, 2515
SmoI CTYRAG 3 cut(s) 1410, 1765, 2515
SseBI AGGCCT 1 cut(s) 2002
SsiI CCGC 8 cut(s) 18, 270, 661, 950, 1160, 1175, 1729, 2126
SspDI GGCGCC 1 cut(s) 239
SspI AATATT 1 cut(s) 1717
SspMI CTAG 3 cut(s) 32, 1472, 2130
SstI GAGCTC 2 cut(s) 1083, 2241
StuI AGGCCT 1 cut(s) 2002
StyD4I CCNGG 9 cut(s) 79, 189, 353, 423, 626, 1053, 1190, 1629, 2110
StyI CCWWGG 3 cut(s) 31, 1042, 1431
TaaI ACNGT 6 cut(s) 331, 778, 976, 1109, 1387, 1559
TaiI ACGT 4 cut(s) 228, 702, 1298, 1835
TaqI TCGA 6 cut(s) 174, 204, 632, 1275, 1766, 1836
TaqII GACCGA 2 cut(s) 398, 1967
TatI WGTACW 4 cut(s) 762, 780, 1476, 2187
TauI GCSGC 1 cut(s) 2129
TfiI GAWTC 6 cut(s) 122, 488, 714, 923, 1610, 2170
Tru1I TTAA 7 cut(s) 165, 1245, 1439, 1920, 2138, 2148, 2271
Tru9I TTAA 7 cut(s) 165, 1245, 1439, 1920, 2138, 2148, 2271
TseFI GTSAC 3 cut(s) 349, 410, 1291
TseI GCWGC 3 cut(s) 450, 620, 2014
Tsp45I GTSAC 3 cut(s) 349, 410, 1291
TspDTI ATGAA 2 cut(s) 2162, 2381
TspGWI ACGGA 5 cut(s) 151, 462, 741, 784, 2231
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 4 cut(s) 1060, 1504, 1865, 2348
VneI GTGCAC 1 cut(s) 772
VpaK11BI GGWCC 5 cut(s) 385, 501, 1049, 1115, 1950
XagI CCTNNNNNAGG 2 cut(s) 2368, 2518
XapI RAATTY 2 cut(s) 1789, 2273
XceI RCATGY 5 cut(s) 211, 1100, 1981, 2147, 2270
XhoI CTCGAG 1 cut(s) 1765
XmaJI CCTAGG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 632, 2178
XspI CTAG 3 cut(s) 32, 1472, 2130
ZraI GACGTC 1 cut(s) 226
ZrmI AGTACT 1 cut(s) 2189
Zsp2I ATGCAT 2 cut(s) 1983, 2323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.