MD15G1037900.v1.1

Electron transfer DM13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014477
Physical Location & Seq
Reverse (-)
2651026 .. 2653830
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1037900.v1.1.491

Sequence Viewer

Length: 2721 bp
ATGCCTCGCAAACCCAATTTCCTAGGGTTTCTCTCCGTCCTCCTCCTCCTCCTCGCATTCAGCCATGCCGATCCGGGTTGCCCCAAGACCAGCCCCCTCGTCAACTCTGAATCCGAATTCAAAATGCTCCAGCATCAGCTCCGCGGCTCCATTCAGATAATCGATGATTGCTCCTTCAAGGTCTCCGATTTCGACATGCTCCCTGGCTCCGACGTTCACTGGTGGGGCGCTGCGGCTCCCGATTTCACCAACCTCACCGCCGGCTTCGTCGTCTCCGATCAGAAGCTCAACGAGACGTACAAAAGCGCCAGCTTTATCGTGCGCTTGACGGAAAATGTCACTTGGGAACGGATCCAGGTCCTCGCCGCGTGGGACGTCCCCACCGCTTCCGACTTCGGGCACGTAATTCTCGGGGATTTCAGAAACGCGTCGTCCGAATCGGCTCCTTCTCCGTCGCCGTCGAGTGGAACCAATTCAGGGAACGGAAGGAATCGGGTTCGGGTTCATACCGAGCCGACGATGCTGGAGAATTGCAAGGTTTTGTCGAAGAATTATAGGGTTCGATGGACGTTGAGTGCTGAGGAGAATACGATCGATATCGGGTTGGAGGCCGCCACAGGGACCATGAATTACATGGCTTTCGGCTGGGCCAACCCGAATTCAACTTCCGAGCTCATGCTCGGAGCCGATGTCGCTGTCGCCGGGTTTAAAGAGGACCGCATGCCGTTTGCTAACGATTTCTACATCACAAAGTACAGTGAGTGTACGTTGTCCAAGGATGGCGAGGTGAAAGGGGTTTGCCCCGATACCAGATACGAAGGGTCCGGCCCGAATGGCAGAGAGGTGAACAATACCAAATTGGTTTACGGGCAGAGGAGGGACGCCGTGTCGTTTATTAGGTACCAGAGGCCGTTGGTTTCCGACGACAAGAAGTACGATTTGGCGGTGAATTATACGGAGAAGATGACGGTCATTTGGGCATTAGGGCCGATTAGGCCACCGGATCTTCTTCAGCCTTACTATCTTCCCCAGAACCATGGTGGACCGAAGAGTGTGGTTTTTGGGCATTTGGTGCTGAATGTTTCTGAAAATGTGAATGACTGTTTGGGTCCTTTAGATGCAGATGAAAAGGAGGATCAGCATCTCATTATCGCTGATGCCAAGGCCTCGCTAGTTGTTACTTCCAGCCCGGCGTTAAATTATCCAGACCCGCCAAACCCTTCAAAAGTTTTGTACATTAACAAGAAAGAGGCTCCGATGTTGAGAGTGGAAAGAGGGGTGCCCGTCAAGTTCTCGATACAAGCAGGGCATGATGTTGCGATGTACATTACTTCCGACCCGCTTGGTGGGAATGCTACACTAAGGAATACTACCGAGACTATTTATGCAGGAGGCCCTGAAGCTCAAGGAGTTCAAGCCAGTCCTAAGGAATTGGTTTGGGCACCGGATAGGAATACCCCGGACCAAGTATATTATCAATCTCTTTACGAGCAGAAAATGGGTTGGAAAGTGCAGGTGGTTGATGGGGGTCTGCCTGATATGTATAACAACAGTGCTGTTTTGGATGATCAGCAAGTTACCTTGTTCTGGACTTTGTCAGAACACTCAATATCTATTGCAGTTCGTGGTGAGAAGAAGAGCGGTTTTTTAGCAATAGGGTTTGGCAGAGGAATGGTGAACAGCTATTCCTATGTGGGTTGGATTGATAATATTGGTAAAGGGCGGGTAAACACTTACTGGATTAACGGGAAGGATGCTTCGAGTATACATCCAACAACTGAGAATTTGACTCATGTGAGGTGCAAGTCTGAAAATGGCATCATTACATTCGAGTTCACTCGTCCTTTGAAACCGTCATGTGCTAAGAATGATAAACCGGAGTGTAAAAACAGAATAGATCCCACTACTCCACTTAAAGTTGTTTGGGCAATGGGTGCTACGTGGACCGATGAGAATCTAAGTGAACAAAATATGCATTTCATTACAAGCAGTAGGCCTATTAGGGTGATGCTTATACGTGGTTCAGCAGAGGCGGAGCAGGATTTACAGCCGGTATTAGCTGTACATGGATTTATGATGTTTCTCGCTTGGGGTATCTTGCTTCCTGGTGGAATACTGTCGGCTAGATACTTGAAACATGTTAAGGGTGACGGTTGGTTCAAGATTCATGTTTACTTGCAGTACTCAGGTTTGGCAATCATCCTACTTGCCGTTCTTTTTGCTGTTGCTGAGCTTCGGGGTTTCTTTGTCAGCTCATTACATGTTAAGTTTGGGATGGCTGCATTACTTTTGGTCTGTGTACAACCGGTTAATGCATACCTAAGGCCCAAAAGACCAGGTCATGGAGAGGAGGTTTCCTCTAAAAGGATTCTCTGGGAGTACTTTCATGTGATCGGTGGGAGATGCGCCTTTGTTGTAGGAATTGCAGCACTTTTCAGCGGAATGAAGCATTTAGGAGATAGATATGACGGTGAAAATGTTCACGGACTTAATTGGGCATTGATAGTTTGGTTCTTGATGGGTGCATTGATGGTTATGTATCTGGAATACCGTGAAAAACAACAGAGGAGGGATAGAAGTTTTGGAAGAAGCAATTGGGTGCTGGGGAACCTTGAGGAAGATGACTCTGTTGATCTGTTGAGTCCGAACGGAATACACTCGGAGAAAGAATCGCAATCGTCAGGAAGAGTGGAAGTCCAGTTAGAGCCTCTGAACAGATAG

Protein Analysis

907

Amino Acids

100.8

Weight (kDa)

6.22

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DM13 PF10517 39 - 136 3.9e-13 Electron transfer DM13
DOMON PF03351 182 - 329 9.7e-15 DOMON domain
At5g54830 PF25489 383 - 508 1.5e-69 At5g54830-like domain
DOMON PF03351 522 - 645 3.8e-25 DOMON domain
Cytochrom_C_asm PF01578 686 - 855 1.1e-06 Cytochrome C assembly protein
Cytochrom_B561 PF03188 688 - 816 9.4e-07 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54830
malus_domestica MD08G1058600.v1.1 MD15G1037900.v1.1
prunus_persica Prupe.1G391800_v2.0.a1
pyrus_communis pycom08g04850 pycom15g03510
rosa_chinensis RchiOBHm_Chr6g0299641
rosa_laevigata RLG00000011400
rosa_multiflora Rmu_co8233929.1_g000001 Rmu_co8458799.1_g000001 Rmu_sc0002895.1_g000005
rosa_roxburghii Rroxscaffold_7G00168480
rosa_rugosa Rorug06G0291300
rosa_samantha Rh6BG410500 Rh6CG416200 Rh6DG403400
rosa_wichuraiana Rw6G035190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1504
AatII GACGTC 1 cut(s) 378
Acc36I ACCTGC 1 cut(s) 1504
Acc65I GGTACC 1 cut(s) 900
AccB1I GGYRCC 3 cut(s) 900, 1279, 1441
AccB7I CCANNNNNTGG 1 cut(s) 2342
AccBSI CCGCTC 1 cut(s) 1641
AccI GTMKAC 1 cut(s) 1765
AccII CGCG 3 cut(s) 144, 368, 428
AclWI GGATC 6 cut(s) 65, 346, 359, 1011, 1143, 1892
AcsI RAATTY 3 cut(s) 116, 658, 1783
AcuI CTGAAG 2 cut(s) 995, 1419
AcyI GRCGYC 2 cut(s) 375, 882
AfiI CCNNNNNNNGG 8 cut(s) 396, 464, 477, 618, 1346, 1587, 2342, 2364
AflIII ACRYGT 3 cut(s) 426, 2137, 2260
AgeI ACCGGT 1 cut(s) 2305
AgsI TTSAA 8 cut(s) 121, 178, 663, 1224, 1415, 1849, 2134, 2161
AhdI GACNNNNNGTC 1 cut(s) 886
AjnI CCWGG 4 cut(s) 202, 354, 2104, 2335
AjuI GAANNNNNNNTTGG 6 cut(s) 923, 955, 1088, 1120, 2578, 2610
AloI GAACNNNNNNTCC 2 cut(s) 1670, 1702
AluBI AGCT 9 cut(s) 139, 286, 312, 673, 1403, 1683, 2060, 2233, 2253
AluI AGCT 9 cut(s) 139, 286, 312, 673, 1403, 1683, 2060, 2233, 2253
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 4 cut(s) 187, 277, 287, 1370
AlwI GGATC 6 cut(s) 65, 346, 359, 1011, 1143, 1892
Ama87I CYCGRG 1 cut(s) 410
ApeKI GCWGC 3 cut(s) 230, 2279, 2426
ApoI RAATTY 3 cut(s) 116, 658, 1783
AsiGI ACCGGT 1 cut(s) 2305
Asp700I GAANNNNTTC 2 cut(s) 472, 2478
Asp718I GGTACC 1 cut(s) 900
AspA2I CCTAGG 1 cut(s) 22
AspLEI GCGC 4 cut(s) 230, 308, 324, 2408
AsuC2I CCSGG 4 cut(s) 75, 703, 1190, 1460
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 8 cut(s) 358, 621, 715, 822, 1043, 1109, 1462, 1944
AvrII CCTAGG 1 cut(s) 22
AxyI CCTNAGG 2 cut(s) 1425, 2321
BaeGI GKGCMC 3 cut(s) 402, 1284, 1444
BamHI GGATCC 1 cut(s) 351
BanI GGYRCC 3 cut(s) 900, 1279, 1441
BanII GRGCYC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 675
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 3 cut(s) 217, 2266, 2438
BccI CCATC 6 cut(s) 558, 773, 1517, 2269, 2512, 2524
BceAI ACGGC 5 cut(s) 442, 709, 869, 895, 2195
BciT130I CCWGG 4 cut(s) 204, 356, 2106, 2337
BclI TGATCA 1 cut(s) 1567
BcnI CCSGG 4 cut(s) 75, 703, 1190, 1460
BcoDI GTCTC 4 cut(s) 187, 277, 287, 1370
BfaI CTAG 3 cut(s) 23, 1172, 2124
BfoI RGCGCY 2 cut(s) 231, 309
BfuAI ACCTGC 1 cut(s) 1504
BglI GCCNNNNNGGC 2 cut(s) 834, 994
BisI GCNGC 7 cut(s) 145, 231, 234, 366, 612, 2280, 2427
BlnI CCTAGG 1 cut(s) 22
BlpI GCTNAGC 1 cut(s) 2229
BlsI GCNGC 7 cut(s) 146, 232, 235, 367, 613, 2281, 2428
BmcAI AGTACT 2 cut(s) 2183, 2381
Bme1390I CCNGG 8 cut(s) 75, 204, 356, 703, 1190, 1460, 2106, 2337
Bme18I GGWCC 8 cut(s) 358, 621, 715, 822, 1043, 1109, 1462, 1944
BmeRI GACNNNNNGTC 1 cut(s) 886
BmeT110I CYCGRG 1 cut(s) 410
BmrFI CCNGG 8 cut(s) 75, 204, 356, 703, 1190, 1460, 2106, 2337
BmsI GCATC 9 cut(s) 142, 510, 1108, 1147, 1150, 1744, 1827, 1998, 2393
BplI GAGNNNNNCTC 2 cut(s) 2342, 2374
BpmI CTGGAG 2 cut(s) 113, 545
Bpu10I CCTNAGC 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 2229
BpuEI CTTGAG 2 cut(s) 1389, 2633
BpuMI CCSGG 4 cut(s) 75, 703, 1190, 1460
Bsa29I ATCGAT 2 cut(s) 162, 594
BsaAI YACGTR 3 cut(s) 403, 1941, 2018
BsaBI GATNNNNATC 3 cut(s) 596, 1140, 1953
BsaHI GRCGYC 2 cut(s) 375, 882
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 7 cut(s) 22, 142, 202, 774, 1036, 1161, 1458
BsaWI WCCGGW 4 cut(s) 1000, 1444, 1876, 2305
BsaXI ACNNNNNCTCC 4 cut(s) 675, 705, 2337, 2367
Bsc4I CCNNNNNNNGG 8 cut(s) 396, 464, 477, 618, 1346, 1587, 2342, 2364
Bse118I RCCGGY 3 cut(s) 260, 2050, 2305
Bse1I ACTGG 4 cut(s) 224, 1419, 1742, 2698
Bse21I CCTNAGG 2 cut(s) 1425, 2321
Bse3DI GCAATG 1 cut(s) 1935
Bse8I GATNNNNATC 3 cut(s) 596, 1140, 1953
BseBI CCWGG 4 cut(s) 204, 356, 2106, 2337
BseCI ATCGAT 2 cut(s) 162, 594
BseDI CCNNGG 7 cut(s) 22, 142, 202, 774, 1036, 1161, 1458
BseGI GGATG 6 cut(s) 784, 1570, 1759, 1768, 2199, 2280
BseJI GATNNNNATC 3 cut(s) 596, 1140, 1953
BseLI CCNNNNNNNGG 8 cut(s) 396, 464, 477, 618, 1346, 1587, 2342, 2364
BseMI GCAATG 1 cut(s) 1935
BseMII CTCAG 4 cut(s) 570, 1770, 2199, 2220
BseNI ACTGG 4 cut(s) 224, 1419, 1742, 2698
BseRI GAGGAG 8 cut(s) 32, 35, 38, 41, 596, 889, 2363, 2581
BseSI GKGCMC 3 cut(s) 402, 1284, 1444
BseXI GCAGC 3 cut(s) 217, 2266, 2438
BseYI CCCAGC 2 cut(s) 645, 2602
BsgI GTGCAG 1 cut(s) 1532
Bsh1236I CGCG 3 cut(s) 144, 368, 428
Bsh1285I CGRYCG 1 cut(s) 594
BshNI GGYRCC 3 cut(s) 900, 1279, 1441
BshTI ACCGGT 1 cut(s) 2305
BshVI ATCGAT 2 cut(s) 162, 594
BsiEI CGRYCG 1 cut(s) 594
BsiHKAI GWGCWC 1 cut(s) 675
BsiHKCI CYCGRG 1 cut(s) 410
BslFI GGGAC 4 cut(s) 362, 386, 634, 893
BslI CCNNNNNNNGG 8 cut(s) 396, 464, 477, 618, 1346, 1587, 2342, 2364
BsmAI GTCTC 4 cut(s) 187, 277, 287, 1370
BsmBI CGTCTC 2 cut(s) 277, 287
BsmFI GGGAC 4 cut(s) 362, 386, 634, 893
BsmI GAATGC 2 cut(s) 56, 1357
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 4 cut(s) 402, 675, 1284, 1444
Bsp1407I TGTACA 4 cut(s) 1233, 1323, 2062, 2299
Bsp1720I GCTNAGC 1 cut(s) 2229
Bsp19I CCATGG 1 cut(s) 1036
BspCNI CTCAG 4 cut(s) 571, 1771, 2198, 2221
BspDI ATCGAT 2 cut(s) 162, 594
BspFNI CGCG 3 cut(s) 144, 368, 428
BspMI ACCTGC 1 cut(s) 1504
BspPI GGATC 6 cut(s) 65, 346, 359, 1011, 1143, 1892
BspQI GCTCTTC 1 cut(s) 1631
BspT107I GGYRCC 3 cut(s) 900, 1279, 1441
BspTNI GGTCTC 1 cut(s) 187
BsrBI CCGCTC 1 cut(s) 1641
BsrDI GCAATG 1 cut(s) 1935
BsrFI RCCGGY 3 cut(s) 260, 2050, 2305
BsrGI TGTACA 4 cut(s) 1233, 1323, 2062, 2299
BsrI ACTGG 4 cut(s) 224, 1419, 1742, 2698
BssAI RCCGGY 3 cut(s) 260, 2050, 2305
BssECI CCNNGG 7 cut(s) 22, 142, 202, 774, 1036, 1161, 1458
BssNAI GTATAC 1 cut(s) 1766
BssNI GRCGYC 2 cut(s) 375, 882
BssT1I CCWWGG 4 cut(s) 22, 774, 1036, 1161
Bst1107I GTATAC 1 cut(s) 1766
Bst2UI CCWGG 4 cut(s) 204, 356, 2106, 2337
Bst4CI ACNGT 9 cut(s) 758, 970, 1103, 1553, 1854, 2118, 2153, 2471, 2552
Bst6I CTCTTC 3 cut(s) 1043, 1631, 2680
BstACI GRCGYC 2 cut(s) 375, 882
BstAPI GCANNNNNTGC 2 cut(s) 1072, 1934
BstAUI TGTACA 4 cut(s) 1233, 1323, 2062, 2299
BstBAI YACGTR 3 cut(s) 403, 1941, 2018
BstC8I GCNNGC 3 cut(s) 262, 310, 722
BstDEI CTNAG 9 cut(s) 579, 1361, 1425, 1779, 1863, 1958, 2185, 2229, 2321
BstDSI CCRYGG 2 cut(s) 142, 1036
BstENI CCTNNNNNAGG 1 cut(s) 2362
BstF5I GGATG 6 cut(s) 784, 1570, 1759, 1768, 2199, 2280
BstFNI CGCG 3 cut(s) 144, 368, 428
BstH2I RGCGCY 2 cut(s) 231, 309
BstHHI GCGC 4 cut(s) 230, 308, 324, 2408
BstMAI GTCTC 4 cut(s) 187, 277, 287, 1370
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 6 cut(s) 520, 692, 834, 994, 1072, 1934
BstNI CCWGG 4 cut(s) 204, 356, 2106, 2337
BstNSI RCATGY 4 cut(s) 199, 724, 2141, 2264
BstSCI CCNGG 8 cut(s) 73, 202, 354, 701, 1188, 1458, 2104, 2335
BstSLI GKGCMC 3 cut(s) 402, 1284, 1444
BstUI CGCG 3 cut(s) 144, 368, 428
BstV1I GCAGC 3 cut(s) 217, 2266, 2438
BstX2I RGATCY 3 cut(s) 351, 1003, 1897
BstXI CCANNNNNNTGG 1 cut(s) 1037
BstYI RGATCY 3 cut(s) 351, 1003, 1897
BstZ17I GTATAC 1 cut(s) 1766
Bsu15I ATCGAT 2 cut(s) 162, 594
Bsu36I CCTNAGG 2 cut(s) 1425, 2321
BsuTUI ATCGAT 2 cut(s) 162, 594
BtgI CCRYGG 2 cut(s) 142, 1036
BtgZI GCGATG 1 cut(s) 1334
BtsCI GGATG 6 cut(s) 784, 1570, 1759, 1768, 2199, 2280
BtsIMutI CAGTG 3 cut(s) 217, 763, 1558
BveI ACCTGC 1 cut(s) 1504
Cac8I GCNNGC 3 cut(s) 262, 310, 722
CfoI GCGC 4 cut(s) 230, 308, 324, 2408
Cfr10I RCCGGY 3 cut(s) 260, 2050, 2305
Cfr42I CCGCGG 1 cut(s) 145
ClaI ATCGAT 2 cut(s) 162, 594
CseI GACGC 2 cut(s) 417, 890
CsiI ACCWGGT 1 cut(s) 2335
CspAI ACCGGT 1 cut(s) 2305
DdeI CTNAG 9 cut(s) 579, 1361, 1425, 1779, 1863, 1958, 2185, 2229, 2321
DraI TTTAAA 1 cut(s) 709
DriI GACNNNNNGTC 1 cut(s) 886
Eam1104I CTCTTC 3 cut(s) 1043, 1631, 2680
Eam1105I GACNNNNNGTC 1 cut(s) 886
EarI CTCTTC 3 cut(s) 1043, 1631, 2680
EciI GGCGGA 1 cut(s) 2048
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 4 cut(s) 22, 774, 1036, 1161
Eco147I AGGCCT 2 cut(s) 1166, 1996
Eco24I GRGCYC 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 8 cut(s) 358, 621, 715, 822, 1043, 1109, 1462, 1944
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 2 cut(s) 995, 1419
Eco81I CCTNAGG 2 cut(s) 1425, 2321
Eco88I CYCGRG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 673
EcoNI CCTNNNNNAGG 1 cut(s) 2362
EcoO109I RGGNCCY 3 cut(s) 358, 1109, 1394
EcoRI GAATTC 2 cut(s) 116, 658
EcoRII CCWGG 4 cut(s) 202, 354, 2104, 2335
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 4 cut(s) 22, 774, 1036, 1161
EcoT22I ATGCAT 2 cut(s) 1977, 2317
EcoT38I GRGCYC 1 cut(s) 675
ErhI CCWWGG 4 cut(s) 22, 774, 1036, 1161
Esp3I CGTCTC 2 cut(s) 277, 287
FaqI GGGAC 4 cut(s) 362, 386, 634, 893
FauI CCCGC 3 cut(s) 1218, 1347, 1716
FbaI TGATCA 1 cut(s) 1567
FblI GTMKAC 1 cut(s) 1765
Fnu4HI GCNGC 7 cut(s) 145, 231, 234, 366, 612, 2280, 2427
FokI GGATG 6 cut(s) 791, 1577, 1755, 1766, 2186, 2287
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 7 cut(s) 145, 231, 234, 366, 612, 2280, 2427
FspBI CTAG 3 cut(s) 23, 1172, 2124
GlaI GCGC 4 cut(s) 229, 307, 323, 2407
GluI GCNGC 7 cut(s) 145, 231, 234, 366, 612, 2280, 2427
GsaI CCCAGC 2 cut(s) 649, 2606
GsuI CTGGAG 2 cut(s) 113, 545
HaeII RGCGCY 2 cut(s) 231, 309
HgaI GACGC 2 cut(s) 417, 890
HhaI GCGC 4 cut(s) 230, 308, 324, 2408
Hin1I GRCGYC 2 cut(s) 375, 882
Hin6I GCGC 4 cut(s) 228, 306, 322, 2406
HinP1I GCGC 4 cut(s) 228, 306, 322, 2406
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
Hpy188III TCNNGA 8 cut(s) 239, 1205, 1294, 1588, 2161, 2515, 2543, 2682
Hpy99I CGWCG 7 cut(s) 215, 272, 433, 457, 463, 520, 926
HpyAV CCTTC 6 cut(s) 184, 456, 480, 812, 1230, 1744
HpyCH4III ACNGT 9 cut(s) 758, 970, 1103, 1553, 1854, 2118, 2153, 2471, 2552
HpyCH4IV ACGT 8 cut(s) 213, 296, 375, 402, 569, 767, 1940, 2017
HpyF10VI GCNNNNNNNGC 6 cut(s) 520, 692, 834, 994, 1072, 1934
HpyF3I CTNAG 9 cut(s) 579, 1361, 1425, 1779, 1863, 1958, 2185, 2229, 2321
HpySE526I ACGT 8 cut(s) 213, 296, 375, 402, 569, 767, 1940, 2017
Hsp92I GRCGYC 2 cut(s) 375, 882
HspAI GCGC 4 cut(s) 228, 306, 322, 2406
KpnI GGTACC 1 cut(s) 904
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 1567
KspI CCGCGG 1 cut(s) 145
LguI GCTCTTC 1 cut(s) 1631
Lsp1109I GCAGC 3 cut(s) 217, 2266, 2438
LweI GCATC 9 cut(s) 142, 510, 1108, 1147, 1150, 1744, 1827, 1998, 2393
MabI ACCWGGT 1 cut(s) 2335
MaeI CTAG 3 cut(s) 23, 1172, 2124
MaeII ACGT 8 cut(s) 213, 296, 375, 402, 569, 767, 1940, 2017
MaeIII GTNAC 4 cut(s) 337, 1177, 1576, 2147
MbiI CCGCTC 1 cut(s) 1641
MfeI CAATTG 1 cut(s) 2593
MflI RGATCY 3 cut(s) 351, 1003, 1897
MhlI GDGCHC 4 cut(s) 402, 675, 1284, 1444
MluI ACGCGT 1 cut(s) 426
MlyI GAGTC 3 cut(s) 1783, 2618, 2650
MmeI TCCRAC 8 cut(s) 234, 414, 585, 945, 1359, 1484, 1679, 1796
Mph1103I ATGCAT 2 cut(s) 1977, 2317
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 472, 2478
MseI TTAA 9 cut(s) 708, 1196, 1239, 1743, 1914, 2142, 2265, 2310, 2490
MspA1I CMGCKG 2 cut(s) 144, 2439
MspR9I CCNGG 8 cut(s) 75, 204, 356, 703, 1190, 1460, 2106, 2337
MunI CAATTG 1 cut(s) 2593
Mva1269I GAATGC 2 cut(s) 56, 1357
MvaI CCWGG 4 cut(s) 204, 356, 2106, 2337
MvnI CGCG 3 cut(s) 144, 368, 428
MwoI GCNNNNNNNGC 6 cut(s) 520, 692, 834, 994, 1072, 1934
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 4 cut(s) 75, 703, 1190, 1460
NcoI CCATGG 1 cut(s) 1036
NgoMIV GCCGGC 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 337, 2147
NsiI ATGCAT 2 cut(s) 1977, 2317
NspI RCATGY 4 cut(s) 199, 724, 2141, 2264
PaeI GCATGC 1 cut(s) 724
PaqCI CACCTGC 1 cut(s) 1504
PceI AGGCCT 2 cut(s) 1166, 1996
PciI ACATGT 2 cut(s) 2137, 2260
PciSI GCTCTTC 1 cut(s) 1631
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 432
PctI GAATGC 2 cut(s) 56, 1357
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 2 cut(s) 472, 2478
PfeI GAWTC 7 cut(s) 110, 437, 490, 1954, 2164, 2368, 2669
PflFI GACNNNGTC 2 cut(s) 1594, 2337
PflMI CCANNNNNTGG 1 cut(s) 2342
PinAI ACCGGT 1 cut(s) 2305
PkrI GCNGC 7 cut(s) 146, 232, 235, 367, 613, 2281, 2428
Ple19I CGATCG 1 cut(s) 594
PleI GAGTC 3 cut(s) 1783, 2618, 2649
PpsI GAGTC 3 cut(s) 1783, 2618, 2649
Ppu21I YACGTR 3 cut(s) 403, 1941, 2018
PpuMI RGGWCCY 2 cut(s) 358, 1109
PscI ACATGT 2 cut(s) 2137, 2260
Psp124BI GAGCTC 1 cut(s) 675
Psp5II RGGWCCY 2 cut(s) 358, 1109
Psp6I CCWGG 4 cut(s) 202, 354, 2104, 2335
PspFI CCCAGC 2 cut(s) 645, 2602
PspGI CCWGG 4 cut(s) 202, 354, 2104, 2335
PspPPI RGGWCCY 2 cut(s) 358, 1109
PsuI RGATCY 3 cut(s) 351, 1003, 1897
PsyI GACNNNGTC 2 cut(s) 1594, 2337
PvuI CGATCG 1 cut(s) 594
SacI GAGCTC 1 cut(s) 675
SacII CCGCGG 1 cut(s) 145
SapI GCTCTTC 1 cut(s) 1631
SaqAI TTAA 9 cut(s) 708, 1196, 1239, 1743, 1914, 2142, 2265, 2310, 2490
SatI GCNGC 7 cut(s) 145, 231, 234, 366, 612, 2280, 2427
ScaI AGTACT 2 cut(s) 2183, 2381
SchI GAGTC 3 cut(s) 1783, 2618, 2650
ScrFI CCNGG 8 cut(s) 75, 204, 356, 703, 1190, 1460, 2106, 2337
SduI GDGCHC 4 cut(s) 402, 675, 1284, 1444
SexAI ACCWGGT 1 cut(s) 2335
SfaNI GCATC 9 cut(s) 142, 510, 1108, 1147, 1150, 1744, 1827, 1998, 2393
SfiI GGCCNNNNNGGCC 1 cut(s) 994
Sfr303I CCGCGG 1 cut(s) 145
SgrBI CCGCGG 1 cut(s) 145
SinI GGWCC 8 cut(s) 358, 621, 715, 822, 1043, 1109, 1462, 1944
SmlI CTYRAG 2 cut(s) 1404, 2612
SmoI CTYRAG 2 cut(s) 1404, 2612
SphI GCATGC 1 cut(s) 724
SseBI AGGCCT 2 cut(s) 1166, 1996
SspI AATATT 1 cut(s) 1711
SspMI CTAG 3 cut(s) 23, 1172, 2124
SstI GAGCTC 1 cut(s) 675
StuI AGGCCT 2 cut(s) 1166, 1996
StyD4I CCNGG 8 cut(s) 73, 202, 354, 701, 1188, 1458, 2104, 2335
StyI CCWWGG 4 cut(s) 22, 774, 1036, 1161
TaaI ACNGT 9 cut(s) 758, 970, 1103, 1553, 1854, 2118, 2153, 2471, 2552
TaiI ACGT 8 cut(s) 216, 299, 378, 405, 572, 770, 1943, 2020
TaqI TCGA 9 cut(s) 162, 192, 461, 545, 562, 594, 1295, 1760, 1830
TaqII GACCGA 2 cut(s) 1060, 1961
TatI WGTACW 7 cut(s) 753, 1233, 1323, 2062, 2181, 2299, 2379
TauI GCSGC 4 cut(s) 147, 236, 368, 614
TfiI GAWTC 7 cut(s) 110, 437, 490, 1954, 2164, 2368, 2669
Tru1I TTAA 9 cut(s) 708, 1196, 1239, 1743, 1914, 2142, 2265, 2310, 2490
Tru9I TTAA 9 cut(s) 708, 1196, 1239, 1743, 1914, 2142, 2265, 2310, 2490
TscAI CASTG 3 cut(s) 224, 763, 1558
TseFI GTSAC 2 cut(s) 337, 2147
TseI GCWGC 3 cut(s) 230, 2279, 2426
Tsp45I GTSAC 2 cut(s) 337, 2147
TspDTI ATGAA 7 cut(s) 494, 641, 1140, 1969, 2156, 2375, 2459
TspGWI ACGGA 8 cut(s) 25, 344, 364, 441, 498, 971, 2499, 2664
TspRI CASTG 3 cut(s) 224, 763, 1558
Tth111I GACNNNGTC 2 cut(s) 1594, 2337
Van91I CCANNNNNTGG 1 cut(s) 2342
VpaK11BI GGWCC 8 cut(s) 358, 621, 715, 822, 1043, 1109, 1462, 1944
XagI CCTNNNNNAGG 1 cut(s) 2362
XapI RAATTY 3 cut(s) 116, 658, 1783
XceI RCATGY 4 cut(s) 199, 724, 2141, 2264
XcmI CCANNNNNNNNNTGG 2 cut(s) 631, 1037
XmaJI CCTAGG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 1765
XmnI GAANNNNTTC 2 cut(s) 472, 2478
XspI CTAG 3 cut(s) 23, 1172, 2124
ZraI GACGTC 1 cut(s) 376
ZrmI AGTACT 2 cut(s) 2183, 2381
Zsp2I ATGCAT 2 cut(s) 1977, 2317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.