Rw6G035190

Electron transfer DM13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014477
Physical Location & Seq
Forward (+)
59754170 .. 59758289
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035190.1

Sequence Viewer

Length: 2724 bp
ATGCCTCGCAAGCCCACCGCCACCATCTTCCTAGGGTTTCTCCTCCTCCTCCTCCTTACATTCTGCTATGCCGATCCGGGTTCCAATTGCCCCAAAACCAGCCCTCTCGTCAACTTGGAATCGCAATTCAAAATGCTCCAGCACCAGCTCAGAGGCTCCGTTAAGATACTTGACGATTGCTCCTTCCAGGTTTCCAATTTCGACATGCTCTCCGGCTCCGACGTCCACTGGTGGGGCGCCGTCGCCCCCGATTTCAATAATCTCACCGCCGGCTTCGTAATCTCCGACCAGAAGCTCAACGAGACGTACAAAAGCGCGAGCTTCACTGTGCGCCTGAGGGACAATGTCACCTGGGATCAGATCCAAGTCCTCGCCGTCTGGGACCTCCCCACCTCCTCCGACTTCGGTCACATCGTGCTCCGGGATGTAGTAAACAGATCGTCCGGCAGCTCCGATTTGGCTCCTTCTCCGTCGCCGGCGAGCGATTCAGGGAATGGGACCGGTCAGGTTCACACTGAGCCGACTATGTTGGTGAATTGCAAGAGTTTGTCTGACGATTTTAGGGTTAGATGGACACTGCGTCCTGAGGAGAATGTGATTGACATTGGGCTGGAGGCTGCCACCGGGTCGACGAATTACATGGCTTTCGGGTGGGCCCGCCCGAATATCACACACCAGTTTATGCTCGGAGCTGACGTGGCCGTCGCCGGATTCAAGGAGGACGGAATGCCGTTTGTGGATGATTTTTACATCACAAAGTACAGCGAGTGCACCGTGTACAAGGACGGCTCCGTCAAAGGGGTGTGCCCCGATACAAGATACGAAGGGACGGCCCCGAATGGCTTGGTGAACAATACCAAATTGGTTTACGGCCACCGGAGGGACGCCGTGTCATTTATTAGGTACCAGAGGCCGTTGGAATCAGCTGATAAAAAGTATGATTTGGCGGTGAAGCATACCGAGAAAAGGACGGTGATTTGGGCCGTGGGGCCGATCAGGCCACCGGATACCCTTCAGCCTTACTATCTTCCCCAAAACCATGGGGGACCCCAGGATGTGGTTTATGGCCATTTGGAGCTGAATGTGTCTGAGCATGTGGATGACTGTTATGGTCCTATAGAGGCCGATGACAAGGAGGATCAGCACCTCATTATTGCGGATGCAAAGGCCCCGCTTGTGGTTACTTCCGGGCAGGCAGTGCATTATCCAGACCCTCCGAACCCTTCAAAGGTTCTTTACATTAACAAGAAAGAGGCTCCGGTGTTGAGGGTCGAAAGAGGGGTGCCGGTCACGTTTTCAATACAAGCTGGGCATGATGTTGCTCTCTACATTACTTCAGATCCTCTTGGTGGGAATGCTACACTGAGGAATGCAAGCGAGACTGTGTATGCTGGAGGATCGGAAGCTCAAGGAGTTCAAGCCAGTCCCAAGGAGTTAATTTGGGCACCAGACAGGAATACCCCAGACCTAGTGTACTATCAATCTCTTTATGACCAGAAAATGGGATGGAAGGTACAGGTGGTTGATGGGGGTCTGTCAGATATGTATAATAACAGTGTGATTTTGGATGATCAGCAAGTTACCTTATTTTGGACACTGGCACCTGATTCTATATCTATTGCAGTCCGGGGTGAGAAGAAAAGTGGTTTCGTGGCAATAGGATTTGGTAGGGGAATGGTGAACAGCTATGCTTATGTGGGTTGGATTGATAATATTGGCAAAGGGCGGGTAAATACTTACTGGATTGATGGAAAGGATGCCTCAAGTGTGCATCCAACACGTGAGAATTTGACATATGTGAGGTGCAGGTCAGAAAATGGGATCATTACGTTCGAGTTCACTCGTCCTTTGAAACCATCATGTGGTAAGAGTGATAAGCCCGAGTGTAAAAACATAATTGATCAAACTACTCCTCTTAAAATTATATGGGCAATGGGTGCTACATGGACTGATGATCATCTTGGTGATCAAAATATGCATTTTGCTACCAGCAGTAGGCCTATAAGGGTGCTGCTTATGCGTGGTTCTGCAGAGGCAGAGCAGGATTTACAGCCAGTATTAGCAGTGCATGGATTTATGATGTTTCTCGCTTGGGCTATCTTGCTTCCTGGTGGAATACTGGCGGCTAGATACTTAAAACATGTTAAGGGTGATGGTTGGTACCGGATTCATGTATACTTGCAGTACTCAGGTTTGGTAATCGTTCTACTTGCCCTTCTTTTTGCTGTTGCTGAGCTCCGTGGTTTCTTTTTCGGCTCATTACATGTTAAATTTGGAACTACGGCTATATTTTTGGTTTGTATGCAACCAGTGAATGCATACCTAAGGCCAAAGAGACCAAATAATGGAGAGCAGGTTTCCTCTAAAAGGCTTCTATGGGAGTATCTTCATGTGATCGGGGGCAGATCTGCCATTGTAGTAGGTTTTGGAGCACTTTTCACAGGACTGAAGCATTTAGGTGAGAGATATGGCGGCGAAAATGTTGATGGACTAAATTTAGCTTTGATAGTTTGGTTCTTGATCTGTGTATTGATAGTAATATATCTGGAATACTATGAAAGGCAAAGAAGGAGGAATAGAAGTATCGGCAGAAGCAATTGGGTGCTGGGGAACCTTGAGGAGGATGACTCTGTTGATTTGTTGAGCCCAAATGGAGTACATTCAGAGAAAGAATCACATACATCAGGAAGAATGGAAGTTCAATTAGAGCCTCTGAACAGATAG

Protein Analysis

907

Amino Acids

100.93

Weight (kDa)

6.08

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DM13 PF10517 42 - 139 5.3e-15 Electron transfer DM13
DOMON PF03351 185 - 330 9.9e-12 DOMON domain
At5g54830 PF25489 384 - 509 1.1e-69 At5g54830-like domain
DOMON PF03351 523 - 646 2.4e-24 DOMON domain
Cytochrom_C_asm PF01578 686 - 853 4.9e-06 Cytochrome C assembly protein
Cytochrom_B561 PF03188 689 - 817 4.9e-06 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54830
malus_domestica MD08G1058600.v1.1 MD15G1037900.v1.1
prunus_persica Prupe.1G391800_v2.0.a1
pyrus_communis pycom08g04850 pycom15g03510
rosa_chinensis RchiOBHm_Chr6g0299641
rosa_laevigata RLG00000011400
rosa_multiflora Rmu_co8233929.1_g000001 Rmu_co8458799.1_g000001 Rmu_sc0002895.1_g000005
rosa_roxburghii Rroxscaffold_7G00168480
rosa_rugosa Rorug06G0291300
rosa_samantha Rh6BG410500 Rh6CG416200 Rh6DG403400
rosa_wichuraiana Rw6G035190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 701, 791
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 2 cut(s) 1797, 2344
Acc65I GGTACC 2 cut(s) 903, 2160
AccB1I GGYRCC 6 cut(s) 236, 903, 1282, 1444, 1600, 2160
AccB7I CCANNNNNTGG 4 cut(s) 1057, 1501, 1862, 2345
AccI GTMKAC 2 cut(s) 629, 2175
AccII CGCG 1 cut(s) 317
AciI CCGC 9 cut(s) 18, 267, 658, 947, 1157, 1172, 1726, 2123, 2472
AclWI GGATC 7 cut(s) 68, 355, 363, 1146, 1334, 1405, 1829
AcoI YGGCCR 3 cut(s) 699, 871, 1066
AcsI RAATTY 3 cut(s) 1786, 2270, 2494
AcuI CTGAAG 3 cut(s) 998, 1320, 2468
AcvI CACGTG 1 cut(s) 1781
AcyI GRCGYC 3 cut(s) 222, 237, 885
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 9 cut(s) 308, 761, 779, 905, 1475, 1515, 2162, 2186, 2658
AflIII ACRYGT 3 cut(s) 1778, 2140, 2263
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 8 cut(s) 130, 256, 715, 1227, 1299, 1418, 1852, 2702
AhdI GACNNNNNGTC 2 cut(s) 579, 889
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 4 cut(s) 186, 350, 1050, 2107
Alw21I GWGCWC 4 cut(s) 420, 773, 2238, 2434
Alw26I GTCTC 3 cut(s) 296, 1373, 2329
Alw44I GTGCAC 1 cut(s) 769
AlwI GGATC 7 cut(s) 68, 355, 363, 1146, 1334, 1405, 1829
Ama87I CYCGRG 1 cut(s) 1880
ApaI GGGCCC 1 cut(s) 658
ApaLI GTGCAC 1 cut(s) 769
ApeKI GCWGC 3 cut(s) 447, 617, 2011
ApoI RAATTY 3 cut(s) 1786, 2270, 2494
AsiGI ACCGGT 1 cut(s) 500
Asp718I GGTACC 2 cut(s) 903, 2160
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 3 cut(s) 239, 317, 333
AsuC2I CCSGG 5 cut(s) 78, 422, 625, 1189, 1628
AvaI CYCGRG 1 cut(s) 1880
AvaII GGWCC 4 cut(s) 382, 498, 1046, 1112
AvrII CCTAGG 1 cut(s) 31
AxyI CCTNAGG 3 cut(s) 335, 585, 2324
BaeGI GKGCMC 4 cut(s) 658, 773, 809, 1447
BalI TGGCCA 1 cut(s) 1068
BanI GGYRCC 6 cut(s) 236, 903, 1282, 1444, 1600, 2160
BanII GRGCYC 3 cut(s) 658, 2238, 2648
BbrPI CACGTG 1 cut(s) 1781
Bbv12I GWGCWC 4 cut(s) 420, 773, 2238, 2434
BbvI GCAGC 3 cut(s) 459, 604, 1998
BccI CCATC 8 cut(s) 32, 564, 1500, 1520, 1742, 1864, 2147, 2480
BciT130I CCWGG 4 cut(s) 188, 352, 1052, 2109
BciVI GTATCC 1 cut(s) 1000
BclI TGATCA 4 cut(s) 1570, 1900, 1954, 1966
BcnI CCSGG 5 cut(s) 78, 422, 625, 1189, 1628
BcoDI GTCTC 3 cut(s) 296, 1373, 2329
BfaI CTAG 3 cut(s) 32, 1469, 2127
BfmI CTRYAG 2 cut(s) 1116, 2028
BfoI RGCGCY 1 cut(s) 240
BfuAI ACCTGC 2 cut(s) 1797, 2344
BfuI GTATCC 1 cut(s) 1000
BglI GCCNNNNNGGC 1 cut(s) 997
BglII AGATCT 1 cut(s) 2405
BisI GCNGC 5 cut(s) 448, 618, 2012, 2124, 2473
BlnI CCTAGG 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 2232
BlsI GCNGC 5 cut(s) 449, 619, 2013, 2125, 2474
BmcAI AGTACT 1 cut(s) 2186
Bme1390I CCNGG 9 cut(s) 78, 188, 352, 422, 625, 1052, 1189, 1628, 2109
Bme18I GGWCC 4 cut(s) 382, 498, 1046, 1112
BmeRI GACNNNNNGTC 2 cut(s) 579, 889
BmeT110I CYCGRG 1 cut(s) 1880
BmgBI CACGTC 1 cut(s) 697
BmrFI CCNGG 9 cut(s) 78, 188, 352, 422, 625, 1052, 1189, 1628, 2109
BmsI GCATC 3 cut(s) 1150, 1747, 1780
BoxI GACNNNNGTC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 2612, 2644
BpmI CTGGAG 3 cut(s) 122, 632, 1413
Bpu1102I GCTNAGC 1 cut(s) 2232
BpuEI CTTGAG 3 cut(s) 1392, 1747, 2636
BpuMI CCSGG 5 cut(s) 78, 422, 625, 1189, 1628
BsaAI YACGTR 1 cut(s) 1781
BsaBI GATNNNNATC 1 cut(s) 1956
BsaHI GRCGYC 3 cut(s) 222, 237, 885
BsaI GGTCTC 1 cut(s) 2329
BsaJI CCNNGG 8 cut(s) 31, 351, 984, 1039, 1050, 1428, 1627, 2239
BsaWI WCCGGW 5 cut(s) 500, 876, 1003, 1258, 2163
BsaXI ACNNNNNCTCC 8 cut(s) 164, 194, 194, 224, 681, 711, 2340, 2370
Bse118I RCCGGY 4 cut(s) 269, 475, 500, 1285
Bse1I ACTGG 8 cut(s) 233, 676, 1422, 1602, 1745, 2054, 2124, 2309
Bse21I CCTNAGG 3 cut(s) 335, 585, 2324
Bse3DI GCAATG 1 cut(s) 1938
Bse8I GATNNNNATC 1 cut(s) 1956
BseBI CCWGG 4 cut(s) 188, 352, 1052, 2109
BseDI CCNNGG 8 cut(s) 31, 351, 984, 1039, 1050, 1428, 1627, 2239
BseJI GATNNNNATC 1 cut(s) 1956
BseMI GCAATG 1 cut(s) 1938
BseMII CTCAG 8 cut(s) 163, 326, 507, 576, 1080, 1355, 2202, 2223
BseNI ACTGG 8 cut(s) 233, 676, 1422, 1602, 1745, 2054, 2124, 2309
BseRI GAGGAG 8 cut(s) 32, 35, 38, 41, 385, 602, 1902, 2633
BseSI GKGCMC 4 cut(s) 658, 773, 809, 1447
BseXI GCAGC 3 cut(s) 459, 604, 1998
BseYI CCCAGC 2 cut(s) 1307, 2605
BsgI GTGCAG 1 cut(s) 1825
Bsh1236I CGCG 1 cut(s) 317
BshNI GGYRCC 6 cut(s) 236, 903, 1282, 1444, 1600, 2160
BshTI ACCGGT 1 cut(s) 500
BsiHKAI GWGCWC 4 cut(s) 420, 773, 2238, 2434
BsiHKCI CYCGRG 1 cut(s) 1880
BslFI GGGAC 7 cut(s) 353, 395, 511, 841, 896, 1059, 1410
BsmAI GTCTC 3 cut(s) 296, 1373, 2329
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 7 cut(s) 353, 395, 511, 841, 896, 1059, 1410
BsmI GAATGC 4 cut(s) 732, 1360, 1375, 2320
Bso31I GGTCTC 1 cut(s) 2329
BsoBI CYCGRG 1 cut(s) 1880
Bsp120I GGGCCC 1 cut(s) 654
Bsp1286I GDGCHC 8 cut(s) 420, 658, 773, 809, 1447, 2238, 2434, 2648
Bsp1407I TGTACA 1 cut(s) 777
Bsp1720I GCTNAGC 1 cut(s) 2232
Bsp19I CCATGG 1 cut(s) 1039
BspACI CCGC 9 cut(s) 18, 267, 658, 947, 1157, 1172, 1726, 2123, 2472
BspCNI CTCAG 8 cut(s) 162, 327, 508, 577, 1081, 1356, 2201, 2224
BspFNI CGCG 1 cut(s) 317
BspMAI CTGCAG 1 cut(s) 2032
BspMI ACCTGC 2 cut(s) 1797, 2344
BspPI GGATC 7 cut(s) 68, 355, 363, 1146, 1334, 1405, 1829
BspT107I GGYRCC 6 cut(s) 236, 903, 1282, 1444, 1600, 2160
BspTNI GGTCTC 1 cut(s) 2329
BsrDI GCAATG 1 cut(s) 1938
BsrFI RCCGGY 4 cut(s) 269, 475, 500, 1285
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 8 cut(s) 233, 676, 1422, 1602, 1745, 2054, 2124, 2309
BssAI RCCGGY 4 cut(s) 269, 475, 500, 1285
BssECI CCNNGG 8 cut(s) 31, 351, 984, 1039, 1050, 1428, 1627, 2239
BssNAI GTATAC 1 cut(s) 2176
BssNI GRCGYC 3 cut(s) 222, 237, 885
BssT1I CCWWGG 3 cut(s) 31, 1039, 1428
Bst1107I GTATAC 1 cut(s) 2176
Bst2UI CCWGG 4 cut(s) 188, 352, 1052, 2109
Bst4CI ACNGT 6 cut(s) 328, 775, 973, 1106, 1384, 1556
BstACI GRCGYC 3 cut(s) 222, 237, 885
BstAPI GCANNNNNTGC 2 cut(s) 1198, 1937
BstAUI TGTACA 1 cut(s) 777
BstBAI YACGTR 1 cut(s) 1781
BstC8I GCNNGC 8 cut(s) 11, 271, 319, 477, 481, 658, 1194, 1375
BstDEI CTNAG 9 cut(s) 149, 335, 516, 585, 1089, 1364, 2188, 2232, 2324
BstDSI CCRYGG 3 cut(s) 984, 1039, 2239
BstENI CCTNNNNNAGG 2 cut(s) 2365, 2618
BstFNI CGCG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 3 cut(s) 239, 317, 333
BstMAI GTCTC 3 cut(s) 296, 1373, 2329
BstMWI GCNNNNNNNGC 6 cut(s) 10, 698, 997, 1198, 1937, 2017
BstNI CCWGG 4 cut(s) 188, 352, 1052, 2109
BstNSI RCATGY 4 cut(s) 208, 1097, 2144, 2267
BstPAI GACNNNNGTC 1 cut(s) 405
BstSCI CCNGG 9 cut(s) 76, 186, 350, 420, 623, 1050, 1187, 1626, 2107
BstSFI CTRYAG 2 cut(s) 1116, 2028
BstSLI GKGCMC 4 cut(s) 658, 773, 809, 1447
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 459, 604, 1998
BstX2I RGATCY 3 cut(s) 360, 1339, 2405
BstXI CCANNNNNNTGG 1 cut(s) 1040
BstYI RGATCY 3 cut(s) 360, 1339, 2405
BstZ17I GTATAC 1 cut(s) 2176
Bsu36I CCTNAGG 3 cut(s) 335, 585, 2324
BsuI GTATCC 1 cut(s) 1000
BtgI CCRYGG 3 cut(s) 984, 1039, 2239
BtrI CACGTC 1 cut(s) 697
BtsI GCAGTG 3 cut(s) 575, 1203, 2070
BveI ACCTGC 2 cut(s) 1797, 2344
Cac8I GCNNGC 8 cut(s) 11, 271, 319, 477, 481, 658, 1194, 1375
CfoI GCGC 3 cut(s) 239, 317, 333
Cfr10I RCCGGY 4 cut(s) 269, 475, 500, 1285
CseI GACGC 2 cut(s) 569, 893
Csp6I GTAC 9 cut(s) 307, 760, 778, 904, 1474, 1514, 2161, 2185, 2657
CspAI ACCGGT 1 cut(s) 500
CviQI GTAC 9 cut(s) 307, 760, 778, 904, 1474, 1514, 2161, 2185, 2657
DdeI CTNAG 9 cut(s) 149, 335, 516, 585, 1089, 1364, 2188, 2232, 2324
DinI GGCGCC 1 cut(s) 238
DraIII CACNNNGTG 1 cut(s) 415
DrdI GACNNNNNNGTC 2 cut(s) 701, 791
DriI GACNNNNNGTC 2 cut(s) 579, 889
DseDI GACNNNNNNGTC 2 cut(s) 701, 791
EaeI YGGCCR 3 cut(s) 699, 871, 1066
Eam1105I GACNNNNNGTC 2 cut(s) 579, 889
Ecl136II GAGCTC 1 cut(s) 2236
Eco130I CCWWGG 3 cut(s) 31, 1039, 1428
Eco147I AGGCCT 1 cut(s) 1999
Eco24I GRGCYC 3 cut(s) 658, 2238, 2648
Eco31I GGTCTC 1 cut(s) 2329
Eco47I GGWCC 4 cut(s) 382, 498, 1046, 1112
Eco53kI GAGCTC 1 cut(s) 2236
Eco57I CTGAAG 3 cut(s) 998, 1320, 2468
Eco72I CACGTG 1 cut(s) 1781
Eco81I CCTNAGG 3 cut(s) 335, 585, 2324
Eco88I CYCGRG 1 cut(s) 1880
EcoICRI GAGCTC 1 cut(s) 2236
EcoNI CCTNNNNNAGG 2 cut(s) 2365, 2618
EcoO109I RGGNCCY 3 cut(s) 382, 1046, 1168
EcoRII CCWGG 4 cut(s) 186, 350, 1050, 2107
EcoT14I CCWWGG 3 cut(s) 31, 1039, 1428
EcoT22I ATGCAT 2 cut(s) 1980, 2320
EcoT38I GRGCYC 3 cut(s) 658, 2238, 2648
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 3 cut(s) 31, 1039, 1428
Esp3I CGTCTC 1 cut(s) 296
FaqI GGGAC 7 cut(s) 353, 395, 511, 841, 896, 1059, 1410
FauI CCCGC 3 cut(s) 665, 1179, 1719
FauNDI CATATG 1 cut(s) 1795
FbaI TGATCA 4 cut(s) 1570, 1900, 1954, 1966
FblI GTMKAC 2 cut(s) 629, 2175
Fnu4HI GCNGC 5 cut(s) 448, 618, 2012, 2124, 2473
FriOI GRGCYC 3 cut(s) 658, 2238, 2648
Fsp4HI GCNGC 5 cut(s) 448, 618, 2012, 2124, 2473
FspBI CTAG 3 cut(s) 32, 1469, 2127
GlaI GCGC 3 cut(s) 238, 316, 332
GluI GCNGC 5 cut(s) 448, 618, 2012, 2124, 2473
GsaI CCCAGC 2 cut(s) 1311, 2609
GsuI CTGGAG 3 cut(s) 122, 632, 1413
HaeII RGCGCY 1 cut(s) 240
HgaI GACGC 2 cut(s) 569, 893
HhaI GCGC 3 cut(s) 239, 317, 333
Hin1I GRCGYC 3 cut(s) 222, 237, 885
Hin6I GCGC 3 cut(s) 237, 315, 331
HinP1I GCGC 3 cut(s) 237, 315, 331
HincII GTYRAC 2 cut(s) 112, 630
HindII GTYRAC 2 cut(s) 112, 630
HinfI GANTC 8 cut(s) 119, 485, 711, 920, 1607, 2167, 2627, 2672
Hpy188III TCNNGA 5 cut(s) 584, 1208, 2518, 2546, 2685
Hpy99I CGWCG 5 cut(s) 224, 245, 475, 634, 707
HpyAV CCTTC 8 cut(s) 193, 474, 818, 1022, 1233, 1504, 2225, 2562
HpyCH4III ACNGT 6 cut(s) 328, 775, 973, 1106, 1384, 1556
HpyCH4IV ACGT 6 cut(s) 222, 305, 696, 1292, 1780, 1829
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 698, 997, 1198, 1937, 2017
HpyF3I CTNAG 9 cut(s) 149, 335, 516, 585, 1089, 1364, 2188, 2232, 2324
HpySE526I ACGT 6 cut(s) 222, 305, 696, 1292, 1780, 1829
Hsp92I GRCGYC 3 cut(s) 222, 237, 885
HspAI GCGC 3 cut(s) 237, 315, 331
KasI GGCGCC 1 cut(s) 236
KflI GGGWCCC 1 cut(s) 1046
KpnI GGTACC 2 cut(s) 907, 2164
KroI GCCGGC 2 cut(s) 269, 475
KroNI GCCGGC 2 cut(s) 271, 477
Ksp22I TGATCA 4 cut(s) 1570, 1900, 1954, 1966
Lsp1109I GCAGC 3 cut(s) 459, 604, 1998
LweI GCATC 3 cut(s) 1150, 1747, 1780
MaeI CTAG 3 cut(s) 32, 1469, 2127
MaeII ACGT 6 cut(s) 222, 305, 696, 1292, 1780, 1829
MaeIII GTNAC 5 cut(s) 346, 407, 1180, 1288, 1579
MboII GAAGA 5 cut(s) 19, 1019, 1648, 2378, 2700
MfeI CAATTG 2 cut(s) 85, 2596
MflI RGATCY 3 cut(s) 360, 1339, 2405
MhlI GDGCHC 8 cut(s) 420, 658, 773, 809, 1447, 2238, 2434, 2648
MlsI TGGCCA 1 cut(s) 1068
MluNI TGGCCA 1 cut(s) 1068
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 1 cut(s) 2621
MmeI TCCRAC 6 cut(s) 243, 309, 423, 897, 1682, 1799
Mox20I TGGCCA 1 cut(s) 1068
Mph1103I ATGCAT 2 cut(s) 1980, 2320
MreI CGCCGGCG 1 cut(s) 475
MroNI GCCGGC 2 cut(s) 269, 475
MscI TGGCCA 1 cut(s) 1068
MseI TTAA 7 cut(s) 162, 1242, 1436, 1917, 2135, 2145, 2268
MslI CAYNNNNRTG 3 cut(s) 1098, 1962, 2457
Msp20I TGGCCA 1 cut(s) 1068
MspA1I CMGCKG 1 cut(s) 926
MspR9I CCNGG 9 cut(s) 78, 188, 352, 422, 625, 1052, 1189, 1628, 2109
MunI CAATTG 2 cut(s) 85, 2596
Mva1269I GAATGC 4 cut(s) 732, 1360, 1375, 2320
MvaI CCWGG 4 cut(s) 188, 352, 1052, 2109
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 6 cut(s) 10, 698, 997, 1198, 1937, 2017
NaeI GCCGGC 2 cut(s) 271, 477
NarI GGCGCC 1 cut(s) 237
NciI CCSGG 5 cut(s) 78, 422, 625, 1189, 1628
NcoI CCATGG 1 cut(s) 1039
NdeI CATATG 1 cut(s) 1795
NgoMIV GCCGGC 2 cut(s) 269, 475
NmuCI GTSAC 3 cut(s) 346, 407, 1288
NsiI ATGCAT 2 cut(s) 1980, 2320
NspI RCATGY 4 cut(s) 208, 1097, 2144, 2267
PceI AGGCCT 1 cut(s) 1999
PciI ACATGT 2 cut(s) 2140, 2263
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 411, 693
PctI GAATGC 4 cut(s) 732, 1360, 1375, 2320
PdiI GCCGGC 2 cut(s) 271, 477
PfeI GAWTC 7 cut(s) 119, 485, 711, 920, 1607, 2167, 2672
PflFI GACNNNGTC 1 cut(s) 344
PflMI CCANNNNNTGG 4 cut(s) 1057, 1501, 1862, 2345
PfoI TCCNGGA 1 cut(s) 420
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 5 cut(s) 449, 619, 2013, 2125, 2474
PleI GAGTC 1 cut(s) 2621
PluTI GGCGCC 1 cut(s) 240
PmaCI CACGTG 1 cut(s) 1781
PmlI CACGTG 1 cut(s) 1781
PpsI GAGTC 1 cut(s) 2621
Ppu21I YACGTR 1 cut(s) 1781
PpuMI RGGWCCY 2 cut(s) 382, 1046
PscI ACATGT 2 cut(s) 2140, 2263
PshAI GACNNNNGTC 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 2238
Psp5II RGGWCCY 2 cut(s) 382, 1046
Psp6I CCWGG 4 cut(s) 186, 350, 1050, 2107
PspCI CACGTG 1 cut(s) 1781
PspFI CCCAGC 2 cut(s) 1307, 2605
PspGI CCWGG 4 cut(s) 186, 350, 1050, 2107
PspOMI GGGCCC 1 cut(s) 654
PspPPI RGGWCCY 2 cut(s) 382, 1046
PstI CTGCAG 1 cut(s) 2032
PsuI RGATCY 3 cut(s) 360, 1339, 2405
PsyI GACNNNGTC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 9 cut(s) 308, 761, 779, 905, 1475, 1515, 2162, 2186, 2658
RsaNI GTAC 9 cut(s) 307, 760, 778, 904, 1474, 1514, 2161, 2185, 2657
RseI CAYNNNNRTG 3 cut(s) 1098, 1962, 2457
SacI GAGCTC 1 cut(s) 2238
SalI GTCGAC 1 cut(s) 628
SaqAI TTAA 7 cut(s) 162, 1242, 1436, 1917, 2135, 2145, 2268
SatI GCNGC 5 cut(s) 448, 618, 2012, 2124, 2473
ScaI AGTACT 1 cut(s) 2186
SchI GAGTC 1 cut(s) 2621
ScrFI CCNGG 9 cut(s) 78, 188, 352, 422, 625, 1052, 1189, 1628, 2109
SduI GDGCHC 8 cut(s) 420, 658, 773, 809, 1447, 2238, 2434, 2648
SfaNI GCATC 3 cut(s) 1150, 1747, 1780
SfcI CTRYAG 2 cut(s) 1116, 2028
SfiI GGCCNNNNNGGCC 1 cut(s) 997
SfoI GGCGCC 1 cut(s) 238
SgrAI CRCCGGYG 1 cut(s) 475
SinI GGWCC 4 cut(s) 382, 498, 1046, 1112
SmiMI CAYNNNNRTG 3 cut(s) 1098, 1962, 2457
SmlI CTYRAG 3 cut(s) 1407, 1762, 2615
SmoI CTYRAG 3 cut(s) 1407, 1762, 2615
SseBI AGGCCT 1 cut(s) 1999
SsiI CCGC 9 cut(s) 18, 267, 658, 947, 1157, 1172, 1726, 2123, 2472
SspDI GGCGCC 1 cut(s) 236
SspI AATATT 1 cut(s) 1714
SspMI CTAG 3 cut(s) 32, 1469, 2127
SstI GAGCTC 1 cut(s) 2238
StuI AGGCCT 1 cut(s) 1999
StyD4I CCNGG 9 cut(s) 76, 186, 350, 420, 623, 1050, 1187, 1626, 2107
StyI CCWWGG 3 cut(s) 31, 1039, 1428
TaaI ACNGT 6 cut(s) 328, 775, 973, 1106, 1384, 1556
TaiI ACGT 6 cut(s) 225, 308, 699, 1295, 1783, 1832
TaqI TCGA 4 cut(s) 201, 629, 1272, 1833
TaqII GACCGA 1 cut(s) 395
TatI WGTACW 5 cut(s) 759, 777, 1473, 2184, 2656
TauI GCSGC 2 cut(s) 2126, 2475
TfiI GAWTC 7 cut(s) 119, 485, 711, 920, 1607, 2167, 2672
Tru1I TTAA 7 cut(s) 162, 1242, 1436, 1917, 2135, 2145, 2268
Tru9I TTAA 7 cut(s) 162, 1242, 1436, 1917, 2135, 2145, 2268
TseFI GTSAC 3 cut(s) 346, 407, 1288
TseI GCWGC 3 cut(s) 447, 617, 2011
Tsp45I GTSAC 3 cut(s) 346, 407, 1288
TspDTI ATGAA 3 cut(s) 2159, 2378, 2571
TspGWI ACGGA 5 cut(s) 148, 459, 738, 781, 2228
Tth111I GACNNNGTC 1 cut(s) 344
Van91I CCANNNNNTGG 4 cut(s) 1057, 1501, 1862, 2345
VneI GTGCAC 1 cut(s) 769
VpaK11BI GGWCC 4 cut(s) 382, 498, 1046, 1112
XagI CCTNNNNNAGG 2 cut(s) 2365, 2618
XapI RAATTY 3 cut(s) 1786, 2270, 2494
XceI RCATGY 4 cut(s) 208, 1097, 2144, 2267
XmaJI CCTAGG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 629, 2175
XspI CTAG 3 cut(s) 32, 1469, 2127
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 1 cut(s) 2186
Zsp2I ATGCAT 2 cut(s) 1980, 2320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.