Rorug06G0291300

Electron transfer DM13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014477
Physical Location & Seq
Forward (+)
44214048 .. 44222380
8333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0291300.1

Sequence Viewer

Length: 4626 bp
ATGGGTCTCACTCTCTTGCTTCACAAAATCTCAGCTTCTTCTTCATCTTCAAACCCAGCTCAGCTGTCCCAGACGACCCCTTTGGGAGCTGTGCAAGGTCTGCCCTTTCTGGAGCTGTCTTCAATTGTCATCAATTTGGCGCTAGTTCTTGCGTTTCTCTTCATCGTTTCGGCCCGGCGGATGGTTGTCTGTCTGGGCCGGATTAGGGTGCTGAAAGACGATTTGGGTTCGAATGGGAACCCAATTCGGCATGATAGTGCTGTTGATAGGGGAATTCAGGAGGTTAGAGTGGGCACAGACTTTAAATTTTCAGTGTTTTGCTGCTTCTATGTGTTGTTTGTGCAAGTTGTGGTGTTGGGTTTTGATGGGATTGGTTTGGTTAGAGGAGGAGGGGGTGGTAGTGGGAGGGTGGTGGTTTGGTCTGTGCTTTGCTTACCAGCTGCACAGGGCTTAGCTTGGTTTGTGATGAGCTTCTCGGCTTTGCATTGTAAGTTCAAGGCGGCCGAGAAGTTCCCATTGCTGTTGAGGGGTTGGTGGGCTGTGTCCTTTGTGCTTTGCTTGTGCACTTTGTATGTTGATGGGAGAGGTTTTGTACAAGAAGGCTCGATACACCTTCATTCTCATGTTGTGGCTAATTTCGCAGTCACACCGGCCCTGGCGTTTCTCTGTTTCGTTGCAATTAGGGGTGTGACTGGTGTGATTGTTTGTAGAAACTCTGAGCTTCAAGAGCCATTGCTCGAAGAAGAAGCAGGGTGTCTTAAGGTTACTCCTTATAGTGATGCTGGGATTTTCAGCTTGGCAACACTATCTTGGATAAACCCTCTTCTTTCAATAGGGGCAAAGAGACCGCTTGAGATCAAGGATATTCCTCTTCTTGCACCACAAGATCGAGCCAAGACCAATTATAAGGTCTTGAATTCAAATTGGGAGAAATTGAAGGCTGCAGAACCTTCAAAGCAGCCTTCTTTAGCCTGGGCAATTCTCAAGTCATTTTGGAAGGAGGCAGCTTGTAATGCCATTTTCGCTGGCTTGAACACTCTTGTTTCATATGTGGGTCCGTATATGATCAGCTATTTCGTTGATTACTTGGGGGGAATAGAGACTTTCCCCCATGAAGGGTATATCCTTGCTGGGACCTTCTTTGCAGCAAAGCTAGTTGAGACCTTAACAACCCGGCAGTGGTATCTTGGGGTGGACATTTTGGGTATGCATGTAAGATCAGCTCTAACGGCAATGGTATACCGAAAGGGACTCAGGCTTTCCAGCTCAGCCAAGCAAAGTCACACTAGTGGAGAGATTGTTAACTACATGGCTGTTGATGTGCAGAGGATAGGGGACTATTCCTGGTATCTCCATGACATATGGATGCTTCCTCTGCAAATTGTTCTTGCCCTTGCAATTTTATACAAGAATGTTGGAATTGCTTCTGTTGCTACATTGATTGCCACCATTATCTCCATTGTTCTTACTGTTCCTTTGGCAAAGATACAAGAAGATTATCAAGACAAGTTGATGACGGCCAAGGATGAAAGAATGAGGAAGACTTCTGAGTGCCTGCGGAACATGAGAATTCTCAAGTTGCAAGCTTGGGAGGACAGATACCGACTGATGTTAGAGGAGATGCGTAGTGTGGAGTTTAAGTATCTACGCAAAGCCCTCTACTCGCAAGCTTTTATCACATTCATGTTCTGGAGCTCTCCTATATTTGTTTCAGCTGTCACTTTCGGTACTTCTATATTTTTGGGTACTCGTCTCACTGCAGGTGGTGTTCTTTCTGCTCTCGCCACTTTCAGGATCCTCCAAGAACCACTCAGGAATTTCCCTGACCTGGTATCAATGATGGCTCAGACAAAAGTTTCCCTTGATCGTATTTCTGGATTCTTGCAGGAGGAAGAGTTACAGGCAGATGCAACAATTGTCCTGCCACGGGGCATCACAAGCACGTCAATAGAAATTAAAGATGGCGTGTTTAGCTGGGACCCTTCCTCTGCAAGACCAACATTATCGGGAATACAAATGAAAGTGGAAAGAGGGATGCGTGTTGCTGTATGTGGAATGGTTGGTTCTGGAAAATCAAGTTTTCTCTCTTGCATCCTCGGGGAGATCCCGAAAATCTCCGGTGAAGTAAAGTTATGTGGTACTGCTGCTTATGTTTCTCAGTCAGCATGGATACAGTCTGGTAATATAGAAGAAAATATTCTTTTTGGCAGCCCAATGGAGAAACCAAAGTACAAGAAGGTTATCCATGCTTGTTCACTGAAAAAGGATTTGGAACTTTTCTCACATGGAGATCAGACCATAATTGGTGATCGGGGTATAAATTTGAGTGGTGGCCAGAAGCAACGTGTGCAGCTTGCAAGGGCACTCTATCAGGACGCTGACATTTATTTACTTGATGACCCCTTCAGTGCAGTTGATGCACACACTGGATCAGAGTTGTTTAAGGAATACATATTGACAGCATTAGAAGACAAAACTGTTGTTTTTGTGACCCATCAAGTCGAATTTCTGCCTTCTGCTGATTTAATCCTGGTTCTTAAAGAAGGGCGCATCATACAAGCAGGAAAATATGATGATCTTTTACAGGCAGGAACTGATTTTAAGACACTAGTCTCAGCTCACCATGATGCAATTGAAGCTATGGATATTCCCAATTATTCATCAGGCGACTCTGATCACAACTTGTGTCCAGATGGCTCTGTTGGACTTGGCAAAAATCCTGATTTACCAAGCAGCAGTGTTGATTGTTTGGCAAAGGAAGTGCAAGAAGGTCCATCAGCTTCAGAGCAGAAAGCAATTAAAGAGAAAAAGAAGGCAAAGCGGGCAAGAAAGAAGCAGCTTGTACAAGAAGAGGAAAGGGTAAGAGGAAGAGTCAGCATGAAGGTTTACTTGTCATATATGGCTGCAGCATATAAAGGATCGCTGATTCCACTCATAATTATTGCACAAGCAATATTTCAATTCCTTCAAATTGCTAGTAGTTGGTGGATGGCTTGGGCGAATCCCCAAACACAGGGAGATCAGCCTAAAGTGAGTGCTATGGTCCTTCTTGGTGTTTACATGGCCCTTGCATTTGGGAGCTCTTGGTTTATATTTGTTAGGGCTGTGTTGGTAGCTACCTTTGGTCTAGAAGCTGCACAGAAATTGTTTCTGAAGATGCTTAGGAGTGTTTTCCGCGCACCAATGTCTTTCTTTGATTCTACTCCTGCTGGACGGATCTTGAATCGTGTATCAATTGATCAAAGTGTGGTGGATCTTGATATTCCTTTTAGACTTGGTGGGTTCGCTTCAACGACAATACAGCTTATAGGCATTGTTGGTGTGATGACAAAAGTTACCTGGCAAGTTTTGCTTCTTGTTGTTCCTATGGCAATTGCTTGTTTGTGGATGCAGAAATACTACATGGCTTCATCAAGGGAATTAGTCCGAATTGTTAGCATTCAGAAATCTCCAATTATCCATCTTTTCGGTGAGTCAATTGCAGGGGCGGCCACAATAAGAGGATTTGGACAGGAAAAAAGGTTCATGAAAAGGAATCTTTATTTCCTTGATTGTTTTGCTCGCCCATTCTTCTGCAGTATTGCTGCTATTGAATGGCTCTGCCTACGGATGGAATTACTCTCGACTTTTGTATTTGCTTTCTGCATGCTTTTACTTGTGAGCTTTCCACATGGAACCATTGATCCAAGCATGGCGGGTCTTGCTGTAACATATGGCTTGAATTTAAATGCACGGTTATCACGGTGGATACTTAGCTTTTGCAAGCTTGAGAACAAAATTATTTCCATAGAAAGGATTTATCAGTACAGTCAAATCCCAAGTGAAGCTCCCCCAGTTATTGAGGACTCTCGTCCTCCAACTAGATGGCCAGAGAATGGAACAATCGAACTACATGATTTAAAGGTTCGTTACAAGGAAAATCTTCCTGTAGTGCTTCATGGGGTAACTTGCACTTTTCCTGGTGGAATGAACATTGGAATTGTTGGGCGTACTGGAAGTGGCAAATCCACTTTGATCCAGGCCTTATTTCGACTGATTGAACCAGCAGCTGGGAGGATCCTTATAGACAAAATAGATATTTCAACGCTCGGTCTTCATGATCTTCGTAGCCGTCTCAGTATCATACCCCAGGATCCTACCTTATTCGAAGGAACTATTAGGGGGAACCTTGATCCCCTTCAAGAGCATTCGGATCATGAAGTTTGGCAGGCACTTGACAAATCTCAGCTTGGAGATGTAATCCGTAAAACAGAGCACAAGCTTGATTCACCAGTGCTAGAAAATGGGGATAATTGGAGCGTGGGGCAGCGACAACTTGTTTCTCTGGGTCGGGCATTGCTTAAGCAGGCCAAAATACTGGTGCTGGATGAAGCAACAGCATCAGTTGATACGCAGACAGACAATCTCATCCAGAAAATTATTCGAACAGAATTTAAGAACTGTACTGTGTGCACTATAGCGCATCGTATACCAACCGTCATTGACAGTGATCTAGTATTGGTTCTGAGTGATGGTCGAGTGGCAGAGTTTGATACTCCTCAACGGCTATTAGAAGATAAATCGTCCATGTTTCTAAAATTGGTAACTGAGTATTCATCAAGGTCAAGTGGTATACCGGATTTTTGA

Protein Analysis

1541

Amino Acids

170.6

Weight (kDa)

8.59

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 339 - 604 9.9e-30 ABC transporter transmembrane region
ABC_tran PF00005 668 - 802 1.4e-18 ABC transporter
ABC_membrane PF00664 978 - 1220 3.8e-29 ABC transporter transmembrane region
ABC_tran PF00005 1312 - 1460 3.2e-27 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54830
malus_domestica MD08G1058600.v1.1 MD15G1037900.v1.1
prunus_persica Prupe.1G391800_v2.0.a1
pyrus_communis pycom08g04850 pycom15g03510
rosa_chinensis RchiOBHm_Chr6g0299641
rosa_laevigata RLG00000011400
rosa_multiflora Rmu_co8233929.1_g000001 Rmu_co8458799.1_g000001 Rmu_sc0002895.1_g000005
rosa_roxburghii Rroxscaffold_7G00168480
rosa_rugosa Rorug06G0291300
rosa_samantha Rh6BG410500 Rh6CG416200 Rh6DG403400
rosa_wichuraiana Rw6G035190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 906
AarI CACCTGC 1 cut(s) 1751
Acc36I ACCTGC 1 cut(s) 1751
AccB7I CCANNNNNTGG 2 cut(s) 3875, 4049
AccI GTMKAC 3 cut(s) 1239, 4468, 4612
AccII CGCG 1 cut(s) 3177
AciI CCGC 8 cut(s) 178, 500, 848, 1558, 2821, 3175, 3488, 3695
AcoI YGGCCR 5 cut(s) 501, 1518, 2332, 3489, 3866
AcsI RAATTY 9 cut(s) 273, 305, 916, 1569, 1816, 2320, 2504, 3721, 4430
AcuI CTGAAG 3 cut(s) 2389, 2766, 3173
AfaI GTAC 9 cut(s) 594, 1729, 1747, 2140, 2231, 2844, 3806, 3991, 4444
AflII CTTAAG 2 cut(s) 758, 4340
AflIII ACRYGT 1 cut(s) 2344
AhlI ACTAGT 2 cut(s) 1286, 2608
AjiI CACGTC 1 cut(s) 1944
AjnI CCWGG 9 cut(s) 654, 971, 1343, 1827, 2529, 3338, 3958, 4017, 4128
AjuI GAANNNNNNNTTGG 4 cut(s) 206, 238, 2252, 2284
AleI CACNNNNGTG 1 cut(s) 1287
AloI GAACNNNNNNTCC 4 cut(s) 3506, 3538, 3667, 3699
Alw21I GWGCWC 5 cut(s) 566, 1697, 3083, 4257, 4454
Alw26I GTCTC 7 cut(s) 11, 838, 1094, 1154, 1757, 2617, 4118
Alw44I GTGCAC 2 cut(s) 562, 4450
AlwNI CAGNNNCTG 2 cut(s) 2594, 4049
Ama87I CYCGRG 1 cut(s) 2096
ApaLI GTGCAC 2 cut(s) 562, 4450
ApoI RAATTY 9 cut(s) 273, 305, 916, 1569, 1816, 2320, 2504, 3721, 4430
ArsI GACNNNNNNTTYG 2 cut(s) 64, 96
Asp700I GAANNNNTTC 3 cut(s) 1423, 2196, 3921
AspLEI GCGC 4 cut(s) 142, 2550, 3179, 4462
AspS9I GGNCC 9 cut(s) 172, 196, 652, 1055, 1134, 1978, 2771, 3043, 3064
AsuC2I CCSGG 2 cut(s) 175, 1174
AsuHPI GGTGA 5 cut(s) 2132, 2318, 2612, 3482, 4260
AsuII TTCGAA 3 cut(s) 230, 4146, 4423
AvaI CYCGRG 1 cut(s) 2096
AvaII GGWCC 5 cut(s) 1055, 1134, 1978, 2771, 3043
BaeGI GKGCMC 4 cut(s) 296, 566, 2365, 4454
BalI TGGCCA 2 cut(s) 2334, 3868
BamHI GGATCC 3 cut(s) 1794, 4056, 4132
BanII GRGCYC 2 cut(s) 1697, 3083
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BbsI GAAGAC 4 cut(s) 111, 1547, 2475, 4085
Bbv12I GWGCWC 5 cut(s) 566, 1697, 3083, 4257, 4454
BceAI ACGGC 4 cut(s) 1245, 1533, 4095, 4559
BciT130I CCWGG 9 cut(s) 656, 973, 1345, 1829, 2531, 3340, 3960, 4019, 4130
BciVI GTATCC 2 cut(s) 2163, 3741
BclI TGATCA 3 cut(s) 1065, 2674, 3238
BcnI CCSGG 2 cut(s) 175, 1174
BcoDI GTCTC 7 cut(s) 11, 838, 1094, 1154, 1757, 2617, 4118
BcuI ACTAGT 2 cut(s) 1286, 2608
BfaI CTAG 9 cut(s) 143, 1154, 1287, 2609, 2976, 3128, 3861, 4277, 4493
BfmI CTRYAG 6 cut(s) 942, 1758, 2904, 3574, 3927, 4455
BfoI RGCGCY 1 cut(s) 143
BfrI CTTAAG 2 cut(s) 758, 4340
BfuAI ACCTGC 1 cut(s) 1751
BfuI GTATCC 2 cut(s) 2163, 3741
BlpI GCTNAGC 3 cut(s) 60, 451, 1267
Bme18I GGWCC 5 cut(s) 1055, 1134, 1978, 2771, 3043
BmeT110I CYCGRG 1 cut(s) 2096
BmgBI CACGTC 1 cut(s) 1944
BmgT120I GGNCC 9 cut(s) 172, 196, 652, 1055, 1134, 1978, 2771, 3043, 3064
BmrI ACTGGG 1 cut(s) 3827
BmuI ACTGGG 1 cut(s) 3827
BoxI GACNNNNGTC 2 cut(s) 2609, 3849
BpiI GAAGAC 4 cut(s) 111, 1547, 2475, 4085
BpmI CTGGAG 2 cut(s) 131, 1711
Bpu10I CCTNAGC 1 cut(s) 3161
Bpu1102I GCTNAGC 3 cut(s) 60, 451, 1267
Bpu14I TTCGAA 3 cut(s) 230, 4146, 4423
BpuEI CTTGAG 4 cut(s) 872, 968, 1559, 3788
BpuMI CCSGG 2 cut(s) 175, 1174
BsaI GGTCTC 3 cut(s) 11, 838, 1154
BsaJI CCNNGG 6 cut(s) 654, 972, 1521, 1925, 2095, 4128
BsaWI WCCGGW 2 cut(s) 2117, 4615
BsaXI ACNNNNNCTCC 6 cut(s) 1284, 1314, 1625, 1655, 1880, 1910
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 6 cut(s) 697, 2431, 3833, 3997, 4271, 4362
Bse3DI GCAATG 4 cut(s) 515, 731, 1239, 4334
BseBI CCWGG 9 cut(s) 656, 973, 1345, 1829, 2531, 3340, 3960, 4019, 4130
BseDI CCNNGG 6 cut(s) 654, 972, 1521, 1925, 2095, 4128
BseMI GCAATG 4 cut(s) 515, 731, 1239, 4334
BseNI ACTGG 6 cut(s) 697, 2431, 3833, 3997, 4271, 4362
BseRI GAGGAG 4 cut(s) 399, 402, 1631, 4527
BseSI GKGCMC 4 cut(s) 296, 566, 2365, 4454
BseX3I CGGCCG 1 cut(s) 501
BseYI CCCAGC 5 cut(s) 55, 782, 1130, 1974, 4049
BsgI GTGCAG 5 cut(s) 426, 1343, 2369, 2430, 3120
Bsh1236I CGCG 1 cut(s) 3177
Bsh1285I CGRYCG 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 504
BsiHKAI GWGCWC 5 cut(s) 566, 1697, 3083, 4257, 4454
BsiHKCI CYCGRG 1 cut(s) 2096
BsiSI CCGG 6 cut(s) 175, 199, 650, 1174, 2118, 4616
BslFI GGGAC 5 cut(s) 52, 1147, 1263, 1349, 1991
BsmAI GTCTC 7 cut(s) 11, 838, 1094, 1154, 1757, 2617, 4118
BsmBI CGTCTC 2 cut(s) 1757, 4118
BsmFI GGGAC 5 cut(s) 52, 1147, 1263, 1349, 1991
BsmI GAATGC 2 cut(s) 3438, 4186
Bso31I GGTCTC 3 cut(s) 11, 838, 1154
BsoBI CYCGRG 1 cut(s) 2096
Bsp119I TTCGAA 3 cut(s) 230, 4146, 4423
Bsp1286I GDGCHC 7 cut(s) 296, 566, 1697, 2365, 3083, 4257, 4454
Bsp1407I TGTACA 2 cut(s) 592, 2842
Bsp1720I GCTNAGC 3 cut(s) 60, 451, 1267
BspACI CCGC 8 cut(s) 178, 500, 848, 1558, 2821, 3175, 3488, 3695
BspFNI CGCG 1 cut(s) 3177
BspHI TCATGA 3 cut(s) 3525, 4096, 4195
BspMAI CTGCAG 4 cut(s) 946, 1762, 2908, 3578
BspMI ACCTGC 1 cut(s) 1751
BspT104I TTCGAA 3 cut(s) 230, 4146, 4423
BspTI CTTAAG 2 cut(s) 758, 4340
BspTNI GGTCTC 3 cut(s) 11, 838, 1154
BsrDI GCAATG 4 cut(s) 515, 731, 1239, 4334
BsrFI RCCGGY 1 cut(s) 649
BsrGI TGTACA 2 cut(s) 592, 2842
BsrI ACTGG 6 cut(s) 697, 2431, 3833, 3997, 4271, 4362
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 6 cut(s) 654, 972, 1521, 1925, 2095, 4128
BssNAI GTATAC 3 cut(s) 1240, 4469, 4613
BssT1I CCWWGG 1 cut(s) 1521
Bst1107I GTATAC 3 cut(s) 1240, 4469, 4613
Bst2UI CCWGG 9 cut(s) 656, 973, 1345, 1829, 2531, 3340, 3960, 4019, 4130
Bst6I CTCTTC 6 cut(s) 164, 828, 876, 1887, 2844, 2863
BstAFI CTTAAG 2 cut(s) 758, 4340
BstAPI GCANNNNNTGC 4 cut(s) 100, 2347, 2417, 3349
BstAUI TGTACA 2 cut(s) 592, 2842
BstBI TTCGAA 3 cut(s) 230, 4146, 4423
BstDSI CCRYGG 1 cut(s) 1925
BstFNI CGCG 1 cut(s) 3177
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 4 cut(s) 142, 2550, 3179, 4462
BstMAI GTCTC 7 cut(s) 11, 838, 1094, 1154, 1757, 2617, 4118
BstMCI CGRYCG 1 cut(s) 504
BstNI CCWGG 9 cut(s) 656, 973, 1345, 1829, 2531, 3340, 3960, 4019, 4130
BstNSI RCATGY 2 cut(s) 1214, 3649
BstPAI GACNNNNGTC 2 cut(s) 2609, 3849
BstSFI CTRYAG 6 cut(s) 942, 1758, 2904, 3574, 3927, 4455
BstSLI GKGCMC 4 cut(s) 296, 566, 2365, 4454
BstUI CGCG 1 cut(s) 3177
BstV2I GAAGAC 4 cut(s) 111, 1547, 2475, 4085
BstX2I RGATCY 6 cut(s) 1794, 2103, 3216, 3253, 4056, 4132
BstXI CCANNNNNNTGG 2 cut(s) 3864, 4357
BstYI RGATCY 6 cut(s) 1794, 2103, 3216, 3253, 4056, 4132
BstZ17I GTATAC 3 cut(s) 1240, 4469, 4613
BstZI CGGCCG 1 cut(s) 501
BsuI GTATCC 2 cut(s) 2163, 3741
BtgI CCRYGG 1 cut(s) 1925
BtrI CACGTC 1 cut(s) 1944
BtsI GCAGTG 3 cut(s) 1184, 1755, 2743
BtsIMutI CAGTG 9 cut(s) 318, 1184, 1755, 2255, 2413, 2424, 2743, 4278, 4492
BveI ACCTGC 1 cut(s) 1751
CaiI CAGNNNCTG 2 cut(s) 2594, 4049
CciI TCATGA 3 cut(s) 3525, 4096, 4195
CfoI GCGC 4 cut(s) 142, 2550, 3179, 4462
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 9 cut(s) 172, 196, 652, 1055, 1134, 1978, 2771, 3043, 3064
CseI GACGC 1 cut(s) 2384
CsiI ACCWGGT 1 cut(s) 1827
Csp6I GTAC 9 cut(s) 593, 1728, 1746, 2139, 2230, 2843, 3805, 3990, 4443
CviQI GTAC 9 cut(s) 593, 1728, 1746, 2139, 2230, 2843, 3805, 3990, 4443
DraI TTTAAA 3 cut(s) 304, 3726, 3900
EaeI YGGCCR 5 cut(s) 501, 1518, 2332, 3489, 3866
EagI CGGCCG 1 cut(s) 501
Eam1104I CTCTTC 6 cut(s) 164, 828, 876, 1887, 2844, 2863
EarI CTCTTC 6 cut(s) 164, 828, 876, 1887, 2844, 2863
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 2 cut(s) 1695, 3081
EclXI CGGCCG 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 1521
Eco147I AGGCCT 1 cut(s) 4022
Eco24I GRGCYC 2 cut(s) 1697, 3083
Eco31I GGTCTC 3 cut(s) 11, 838, 1154
Eco47I GGWCC 5 cut(s) 1055, 1134, 1978, 2771, 3043
Eco52I CGGCCG 1 cut(s) 501
Eco53kI GAGCTC 2 cut(s) 1695, 3081
Eco57I CTGAAG 3 cut(s) 2389, 2766, 3173
Eco88I CYCGRG 1 cut(s) 2096
EcoICRI GAGCTC 2 cut(s) 1695, 3081
EcoO109I RGGNCCY 2 cut(s) 1134, 1978
EcoRI GAATTC 3 cut(s) 273, 916, 1569
EcoRII CCWGG 9 cut(s) 654, 971, 1343, 1827, 2529, 3338, 3958, 4017, 4128
EcoT14I CCWWGG 1 cut(s) 1521
EcoT22I ATGCAT 1 cut(s) 1212
EcoT38I GRGCYC 2 cut(s) 1697, 3083
ErhI CCWWGG 1 cut(s) 1521
Esp3I CGTCTC 2 cut(s) 1757, 4118
FalI AAGNNNNNCTT 6 cut(s) 1845, 1877, 2873, 2905, 3336, 3368
FaqI GGGAC 5 cut(s) 52, 1147, 1263, 1349, 1991
FauI CCCGC 2 cut(s) 2814, 3688
FauNDI CATATG 3 cut(s) 1048, 1361, 3712
FbaI TGATCA 3 cut(s) 1065, 2674, 3238
FblI GTMKAC 3 cut(s) 1239, 4468, 4612
FriOI GRGCYC 2 cut(s) 1697, 3083
FspBI CTAG 9 cut(s) 143, 1154, 1287, 2609, 2976, 3128, 3861, 4277, 4493
GlaI GCGC 4 cut(s) 141, 2549, 3178, 4461
GsaI CCCAGC 5 cut(s) 59, 786, 1134, 1978, 4053
GsuI CTGGAG 2 cut(s) 131, 1711
HaeII RGCGCY 1 cut(s) 143
HapII CCGG 6 cut(s) 175, 199, 650, 1174, 2118, 4616
HgaI GACGC 1 cut(s) 2384
HhaI GCGC 4 cut(s) 142, 2550, 3179, 4462
Hin6I GCGC 4 cut(s) 140, 2548, 3177, 4460
HinP1I GCGC 4 cut(s) 140, 2548, 3177, 4460
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HindIII AAGCTT 4 cut(s) 1584, 1668, 3764, 4259
HpaI GTTAAC 1 cut(s) 1303
HpaII CCGG 6 cut(s) 175, 199, 650, 1174, 2118, 4616
HphI GGTGA 5 cut(s) 2132, 2318, 2612, 3482, 4260
HpyCH4IV ACGT 2 cut(s) 1943, 2344
HpySE526I ACGT 2 cut(s) 1943, 2344
HspAI GCGC 4 cut(s) 140, 2548, 3177, 4460
KflI GGGWCCC 1 cut(s) 1978
Ksp22I TGATCA 3 cut(s) 1065, 2674, 3238
KspAI GTTAAC 1 cut(s) 1303
LmnI GCTCC 6 cut(s) 86, 112, 1692, 3078, 3832, 4296
MabI ACCWGGT 1 cut(s) 1827
MaeI CTAG 9 cut(s) 143, 1154, 1287, 2609, 2976, 3128, 3861, 4277, 4493
MaeII ACGT 2 cut(s) 1943, 2344
MfeI CAATTG 6 cut(s) 123, 1914, 2631, 3234, 3372, 3477
MflI RGATCY 6 cut(s) 1794, 2103, 3216, 3253, 4056, 4132
MhlI GDGCHC 7 cut(s) 296, 566, 1697, 2365, 3083, 4257, 4454
MlsI TGGCCA 2 cut(s) 2334, 3868
MluNI TGGCCA 2 cut(s) 2334, 3868
MlyI GAGTC 5 cut(s) 1245, 2663, 2880, 3482, 3839
MmeI TCCRAC 3 cut(s) 1396, 2683, 3881
Mox20I TGGCCA 2 cut(s) 2334, 3868
Mph1103I ATGCAT 1 cut(s) 1212
MroXI GAANNNNTTC 3 cut(s) 1423, 2196, 3921
MscI TGGCCA 2 cut(s) 2334, 3868
MslI CAYNNNNRTG 6 cut(s) 255, 621, 1287, 1364, 1682, 2625
Msp20I TGGCCA 2 cut(s) 2334, 3868
MspA1I CMGCKG 4 cut(s) 64, 440, 1715, 4049
MspCI CTTAAG 2 cut(s) 758, 4340
MspI CCGG 6 cut(s) 175, 199, 650, 1174, 2118, 4616
MunI CAATTG 6 cut(s) 123, 1914, 2631, 3234, 3372, 3477
Mva1269I GAATGC 2 cut(s) 3438, 4186
MvaI CCWGG 9 cut(s) 656, 973, 1345, 1829, 2531, 3340, 3960, 4019, 4130
MvnI CGCG 1 cut(s) 3177
NciI CCSGG 2 cut(s) 175, 1174
NdeI CATATG 3 cut(s) 1048, 1361, 3712
NmeAIII GCCGAG 2 cut(s) 455, 529
NmuCI GTSAC 5 cut(s) 643, 688, 1280, 1717, 2488
NsiI ATGCAT 1 cut(s) 1212
NspI RCATGY 2 cut(s) 1214, 3649
NspV TTCGAA 3 cut(s) 230, 4146, 4423
OliI CACNNNNGTG 1 cut(s) 1287
PaeI GCATGC 1 cut(s) 3649
PagI TCATGA 3 cut(s) 3525, 4096, 4195
PaqCI CACCTGC 1 cut(s) 1751
PceI AGGCCT 1 cut(s) 4022
PctI GAATGC 2 cut(s) 3438, 4186
PdmI GAANNNNTTC 3 cut(s) 1423, 2196, 3921
PfeI GAWTC 7 cut(s) 1878, 2926, 3001, 3197, 3223, 3535, 4265
PflMI CCANNNNNTGG 2 cut(s) 3875, 4049
PleI GAGTC 5 cut(s) 1245, 2663, 2879, 3481, 3839
PpsI GAGTC 5 cut(s) 1245, 2663, 2879, 3481, 3839
PpuMI RGGWCCY 2 cut(s) 1134, 1978
PshAI GACNNNNGTC 2 cut(s) 2609, 3849
PsiI TTATAA 1 cut(s) 906
Psp124BI GAGCTC 2 cut(s) 1697, 3083
Psp5II RGGWCCY 2 cut(s) 1134, 1978
Psp6I CCWGG 9 cut(s) 654, 971, 1343, 1827, 2529, 3338, 3958, 4017, 4128
PspFI CCCAGC 5 cut(s) 55, 782, 1130, 1974, 4049
PspGI CCWGG 9 cut(s) 654, 971, 1343, 1827, 2529, 3338, 3958, 4017, 4128
PspPI GGNCC 9 cut(s) 172, 196, 652, 1055, 1134, 1978, 2771, 3043, 3064
PspPPI RGGWCCY 2 cut(s) 1134, 1978
PstI CTGCAG 4 cut(s) 946, 1762, 2908, 3578
PstNI CAGNNNCTG 2 cut(s) 2594, 4049
PsuI RGATCY 6 cut(s) 1794, 2103, 3216, 3253, 4056, 4132
PvuII CAGCTG 4 cut(s) 64, 440, 1715, 4049
RsaI GTAC 9 cut(s) 594, 1729, 1747, 2140, 2231, 2844, 3806, 3991, 4444
RsaNI GTAC 9 cut(s) 593, 1728, 1746, 2139, 2230, 2843, 3805, 3990, 4443
RseI CAYNNNNRTG 6 cut(s) 255, 621, 1287, 1364, 1682, 2625
SacI GAGCTC 2 cut(s) 1697, 3083
Sau96I GGNCC 9 cut(s) 172, 196, 652, 1055, 1134, 1978, 2771, 3043, 3064
SchI GAGTC 5 cut(s) 1245, 2663, 2880, 3482, 3839
SduI GDGCHC 7 cut(s) 296, 566, 1697, 2365, 3083, 4257, 4454
SexAI ACCWGGT 1 cut(s) 1827
SfcI CTRYAG 6 cut(s) 942, 1758, 2904, 3574, 3927, 4455
SfuI TTCGAA 3 cut(s) 230, 4146, 4423
SinI GGWCC 5 cut(s) 1055, 1134, 1978, 2771, 3043
SmiI ATTTAAAT 1 cut(s) 3726
SmiMI CAYNNNNRTG 6 cut(s) 255, 621, 1287, 1364, 1682, 2625
SmlI CTYRAG 6 cut(s) 758, 851, 983, 1574, 3767, 4340
SmoI CTYRAG 6 cut(s) 758, 851, 983, 1574, 3767, 4340
SpeI ACTAGT 2 cut(s) 1286, 2608
SphI GCATGC 1 cut(s) 3649
SseBI AGGCCT 1 cut(s) 4022
SsiI CCGC 8 cut(s) 178, 500, 848, 1558, 2821, 3175, 3488, 3695
SspI AATATT 2 cut(s) 2197, 2955
SspMI CTAG 9 cut(s) 143, 1154, 1287, 2609, 2976, 3128, 3861, 4277, 4493
SstI GAGCTC 2 cut(s) 1697, 3083
StuI AGGCCT 1 cut(s) 4022
StyI CCWWGG 1 cut(s) 1521
SwaI ATTTAAAT 1 cut(s) 3726
TaiI ACGT 2 cut(s) 1946, 2347
TaqII GACCGA 1 cut(s) 4079
TatI WGTACW 5 cut(s) 592, 2229, 2842, 3804, 4442
TauI GCSGC 2 cut(s) 503, 3491
TfiI GAWTC 7 cut(s) 1878, 2926, 3001, 3197, 3223, 3535, 4265
TscAI CASTG 9 cut(s) 318, 1184, 1762, 2262, 2413, 2431, 2743, 4278, 4492
TseFI GTSAC 5 cut(s) 643, 688, 1280, 1717, 2488
Tsp45I GTSAC 5 cut(s) 643, 688, 1280, 1717, 2488
TspGWI ACGGA 4 cut(s) 1047, 3229, 3622, 4232
TspRI CASTG 9 cut(s) 318, 1184, 1762, 2262, 2413, 2431, 2743, 4278, 4492
Van91I CCANNNNNTGG 2 cut(s) 3875, 4049
Vha464I CTTAAG 2 cut(s) 758, 4340
VneI GTGCAC 2 cut(s) 562, 4450
VpaK11BI GGWCC 5 cut(s) 1055, 1134, 1978, 2771, 3043
XapI RAATTY 9 cut(s) 273, 305, 916, 1569, 1816, 2320, 2504, 3721, 4430
XbaI TCTAGA 1 cut(s) 3127
XceI RCATGY 2 cut(s) 1214, 3649
XmiI GTMKAC 3 cut(s) 1239, 4468, 4612
XmnI GAANNNNTTC 3 cut(s) 1423, 2196, 3921
XspI CTAG 9 cut(s) 143, 1154, 1287, 2609, 2976, 3128, 3861, 4277, 4493
Zsp2I ATGCAT 1 cut(s) 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.