Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009656

Physical Location & Seq

Genomic Coordinates Reverse (-)

5568692 .. 5574005

Gene Structure 5314 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1070600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2262 bp

ATGGCGATCCAGTTCAAATTCAGAAGCTCCGTCAACTTCGATTCCGTCGCCATCGACGGCCGAGCCTCCATCTCCGTTCGCGATCTCAAATCCAAGGTAATTCGCCACAAGAACCTCAACCTCTGCCAAGACTCCGATCTCCTCTTCTCTGACGCCGTAACCGGTCAAGAGTACAACGACGAGAATATTCAAATTCCGTGTGGTTCAAGCGTGATAATTAAGAGAGTTCCTGCAGGATCAGTACATGTGGATCTGCCTCACTTCAACTCGTGCCCGAATCTTCATAGCAAGGACTCTGTCGACGATAAATCACTTGTTGCAGCGAATGCTGAGACTGTTGACTTTGATGACTTTGGGGTTGACGTGTATCCCATTCCTAAAGAAACCGTTTTCAGTTCTGATTTGGATCCAGACAAGGATGACTTCATCTATGATTATCAACCAAATAGCGATAACTGCATCACAAGGTATTCGGAGCCCGCTGTGAGAGGATGCCTGAAACTTGAAGCAAGTGGCATTAGTGATGCTATTCCAGGAGGTCATGCACATAGTGGAGTTGAACAAATCATAGTGCCTACAAAATCAAAGCCTGAAGAAGATGACATGAATGTGGAAAGGGTGGGCAGTGCGAATCCCCTAGATACAGGGCATGCTAATTTGTCAACAGAGCTGAAATGCTCTCTATGCAATGCATATTTCAAGGAGGCTGTGATGATACCATGCTGCCAGCACAGTTTTTGTGAGAAATGCATTTCTCAAGTGTTGCGAGAAAAGTCCAGATGTCCCAAGTGTTTTTCGACCAAATGCAGAGTAGAAGATTTGCTGCCGAATGTTTCTCTTAGGCAAGCAATTGAGCATTTCCTAGAATCCCAAAATTTAATCACTGCTCCAGATAATGATTGTTGCCAATATGCTCCAGATGGAGAATCTGGAATTCAAGCCAAAGATGTGTCTCGCGGCAATAATTTTTTACAGAGAGAGCCAGAGGTGCCTCATTCTCCTGAAACAGGGACAGGATCTAATCATTTCTTCGCAGAATCTGCTTTTAACCCTCCTTTCAAAAAAGATGTTAAACCTGCAATCCCGAAACAAAGGCCACCTTGGGTTTCTTCAGAAGGTTGTGACAAGAGCTTCTTGGAGACTGGAAAGCACAGAAAGGGGAAACGTACCTGTTACATGTGTGGTTCTACAGACCATTTCATAAGAGACTGTCCAGTGGCTTCAAGCCCACATCCTATGCTTCATGCAGGAAATGCAATATTTCCAGGAGCCATGCCAGGTTATGTACCACCATATTGGAATGGAGCTCCCTCACCTCGTGCCATGCCCTTTAGAAATCCATATGGAAATCATGCGATGACGGCGTTTGGTGCAAATATGGTCCCTCCTGCTCCTTATACTATTCCTACATACATGCCATCAATGTATTACTCATTCCCTTCCTTTGGTGGATACATGAGGATGAGAGGTGTTGCTCCTCTTCCAGGGACTAGCAAAGACTGCTATTTAAGTCATTCGGAATCCCTGGATCTTCATGGTGGGGAAAAGAGAAGAAAAGTTTCAAATGAGAATTTGAGGAGAGAACAATCTCATGATTGTGACGATGACGAAGACCATGATTTAAACGAGAGACATGGCTATGATGGGACAGAAAGACTACATGACCACAGATCTTGTATAAACAGGGAAAAGAGCATAAGTTACTCTGAGGAAGGCTCTACTAAAGGGTCCCAGAGGCAATATCGGCTTCATAATCACACAGATGATGACAGGCTCGCAGTTGATGGTCAGTGGAAGAGTTCGCGTTTGGTGGTTGATGGTAGAGCTCCAAAGCAGTATCATCGCACGGAAAGATCAAGCTCAGAATTAGACGATGTGCCAAGTAGCTCTGGTTTCCATGAAGAGAGAAAGAAACACCACCATAGGAGCTCCAGAAAGCACAAGGATCGAAGAGAGCAGTGTGGCAGTGATTCCAGTTGGAGTGATCATCGATATACCAAAGATGATAACAGAAAAGGGATCGAGCATGAATCCAAAAGGCAGAGTCAGAAATATCGCAGCCACTCAGGATCTGGTATGGATCAAAGTGGTTCCAGTGATAAGAAGTTGCAGAAAGAGAGCAGTCACAGTTCTAGACATTCTAAGCACAGTGGAAAGAGTAATGTCAATGGGCGGAGTCATGACAGGTGGAAGATGGTTAGTGGGTCGGATGAAGATTGTGGAGAAGGTTATCAGTATTCTAGACAGAAAAGAAAACACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001701 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006275 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009792 GO:0009888 GO:0009889 GO:0009987 GO:0010556 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0035264 GO:0036211 GO:0040007 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048513 GO:0048568 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051603 GO:0051716 GO:0060255 GO:0060429 GO:0061053 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DWNN zf-C3HC4_2 zf-CCHC

Protein Analysis

754

Amino Acids

84.56

Weight (kDa)

6.79

Isoelectric Point (pI)

48.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DWNN	PF08783	3 - 77	9.4e-21	DWNN domain
zf-C3HC4_2	PF13923	225 - 264	2.1e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	226 - 264	1.5e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120
malus_domestica	MD08G1070600.v1.1 MD15G1051500.v1.1
prunus_persica	Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1
pyrus_communis	pycom08g05840 pycom15g04930
rosa_chinensis	RchiOBHm_Chr6g0311661
rosa_laevigata	RLG00000010383
rosa_roxburghii	Rroxscaffold_7G00157400
rosa_rugosa	Rorug06G0390500.1
rosa_samantha	Rh6AG505800 Rh6BG516600 Rh6CG522500 Rh6DG508200
rosa_wichuraiana	Rw6G044020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1084
AccB1I	GGYRCC	1 cut(s)	988
AccI	GTMKAC	1 cut(s)	300
AccII	CGCG	3 cut(s)	81, 957, 1804
AciI	CCGC	3 cut(s)	480, 957, 2173
AcoI	YGGCCR	1 cut(s)	58
AcsI	RAATTY	5 cut(s)	17, 192, 874, 933, 1570
AcuI	CTGAAG	2 cut(s)	612, 1095
AcyI	GRCGYC	1 cut(s)	153
AdeI	CACNNNGTG	2 cut(s)	551, 1319
AfaI	GTAC	4 cut(s)	173, 243, 1168, 1287
AfiI	CCNNNNNNNGG	3 cut(s)	1007, 1445, 1484
AflIII	ACRYGT	3 cut(s)	244, 363, 1176
AgeI	ACCGGT	1 cut(s)	161
AjiI	CACGTC	1 cut(s)	364
AjnI	CCWGG	5 cut(s)	532, 1264, 1276, 1483, 1524
AluBI	AGCT	8 cut(s)	27, 670, 1131, 1307, 1826, 1860, 1887, 1929
AluI	AGCT	8 cut(s)	27, 670, 1131, 1307, 1826, 1860, 1887, 1929
Alw21I	GWGCWC	3 cut(s)	1309, 1828, 1931
Alw26I	GTCTC	5 cut(s)	326, 957, 1133, 1200, 1624
AlwNI	CAGNNNCTG	2 cut(s)	1040, 2072
AoxI	GGCC	2 cut(s)	58, 1094
ApeKI	GCWGC	4 cut(s)	320, 723, 823, 2058
ApoI	RAATTY	5 cut(s)	17, 192, 874, 933, 1570
AsiGI	ACCGGT	1 cut(s)	161
Asp700I	GAANNNNTTC	1 cut(s)	1558
AspS9I	GGNCC	2 cut(s)	1381, 1728
AsuHPI	GGTGA	1 cut(s)	1305
AvaII	GGWCC	2 cut(s)	1381, 1728
BaeGI	GKGCMC	1 cut(s)	275
BamHI	GGATCC	1 cut(s)	406
BanI	GGYRCC	1 cut(s)	988
BanII	GRGCYC	4 cut(s)	480, 1309, 1828, 1931
BauI	CACGAG	2 cut(s)	268, 1317
BbsI	GAAGAC	1 cut(s)	1617
Bbv12I	GWGCWC	3 cut(s)	1309, 1828, 1931
BbvI	GCAGC	4 cut(s)	332, 710, 810, 2070
BccI	CCATC	8 cut(s)	59, 77, 914, 1426, 1637, 1778, 1811, 2188
BceAI	ACGGC	3 cut(s)	73, 140, 1377
BcgI	CGANNNNNNTGC	4 cut(s)	1823, 1857, 1928, 1962
BciT130I	CCWGG	5 cut(s)	534, 1266, 1278, 1485, 1526
BciVI	GTATCC	2 cut(s)	378, 1445
BclI	TGATCA	1 cut(s)	1984
BcoDI	GTCTC	5 cut(s)	326, 957, 1133, 1200, 1624
BfaI	CTAG	6 cut(s)	638, 863, 1491, 2133, 2241, 2260
BfmI	CTRYAG	2 cut(s)	231, 1188
BfuAI	ACCTGC	1 cut(s)	1084
BfuI	GTATCC	2 cut(s)	378, 1445
BglII	AGATCT	1 cut(s)	1670
BisI	GCNGC	5 cut(s)	321, 724, 824, 958, 2059
BlsI	GCNGC	5 cut(s)	322, 725, 825, 959, 2060
Bme1390I	CCNGG	5 cut(s)	534, 1266, 1278, 1485, 1526
Bme18I	GGWCC	2 cut(s)	1381, 1728
BmgBI	CACGTC	1 cut(s)	364
BmgT120I	GGNCC	2 cut(s)	1381, 1728
BmiI	GGNNCC	8 cut(s)	408, 477, 990, 1270, 1383, 1729, 1730, 2092
BmrFI	CCNGG	5 cut(s)	534, 1266, 1278, 1485, 1526
BmsI	GCATC	3 cut(s)	468, 482, 514
BpiI	GAAGAC	1 cut(s)	1617
BplI	GAGNNNNNCTC	2 cut(s)	1700, 1732
BpmI	CTGGAG	3 cut(s)	873, 900, 1915
BpuEI	CTTGAG	1 cut(s)	741
Bsa29I	ATCGAT	1 cut(s)	1990
BsaBI	GATNNNNATC	1 cut(s)	405
BsaHI	GRCGYC	1 cut(s)	153
BsaJI	CCNNGG	4 cut(s)	93, 1100, 1484, 1524
BsaWI	WCCGGW	1 cut(s)	161
Bsc4I	CCNNNNNNNGG	3 cut(s)	1007, 1445, 1484
Bse118I	RCCGGY	1 cut(s)	161
Bse1I	ACTGG	5 cut(s)	10, 1147, 1214, 1974, 2094
Bse3DI	GCAATG	1 cut(s)	694
Bse8I	GATNNNNATC	1 cut(s)	405
BseBI	CCWGG	5 cut(s)	534, 1266, 1278, 1485, 1526
BseCI	ATCGAT	1 cut(s)	1990
BseDI	CCNNGG	4 cut(s)	93, 1100, 1484, 1524
BseGI	GGATG	5 cut(s)	424, 497, 1231, 1467, 2215
BseJI	GATNNNNATC	1 cut(s)	405
BseLI	CCNNNNNNNGG	3 cut(s)	1007, 1445, 1484
BseMI	GCAATG	1 cut(s)	694
BseMII	CTCAG	4 cut(s)	321, 1698, 1875, 2079
BseNI	ACTGG	5 cut(s)	10, 1147, 1214, 1974, 2094
BseRI	GAGGAG	3 cut(s)	131, 1467, 1591
BseSI	GKGCMC	1 cut(s)	275
BseX3I	CGGCCG	1 cut(s)	58
BseXI	GCAGC	4 cut(s)	332, 710, 810, 2070
Bsh1236I	CGCG	3 cut(s)	81, 957, 1804
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	2 cut(s)	60, 1096
BshNI	GGYRCC	1 cut(s)	988
BshTI	ACCGGT	1 cut(s)	161
BshVI	ATCGAT	1 cut(s)	1990
BsiEI	CGRYCG	1 cut(s)	61
BsiHKAI	GWGCWC	3 cut(s)	1309, 1828, 1931
BsiSI	CCGG	1 cut(s)	162
BslFI	GGGAC	6 cut(s)	768, 1024, 1367, 1501, 1660, 1714
BslI	CCNNNNNNNGG	3 cut(s)	1007, 1445, 1484
BsmAI	GTCTC	5 cut(s)	326, 957, 1133, 1200, 1624
BsmFI	GGGAC	6 cut(s)	768, 1024, 1367, 1501, 1660, 1714
BsmI	GAATGC	1 cut(s)	331
BsnI	GGCC	2 cut(s)	60, 1096
Bsp1286I	GDGCHC	5 cut(s)	275, 480, 1309, 1828, 1931
Bsp68I	TCGCGA	1 cut(s)	81
BspACI	CCGC	3 cut(s)	480, 957, 2173
BspANI	GGCC	2 cut(s)	60, 1096
BspCNI	CTCAG	4 cut(s)	322, 1699, 1874, 2078
BspDI	ATCGAT	1 cut(s)	1990
BspFNI	CGCG	3 cut(s)	81, 957, 1804
BspHI	TCATGA	2 cut(s)	1591, 2179
BspLI	GGNNCC	8 cut(s)	408, 477, 990, 1270, 1383, 1729, 1730, 2092
BspMAI	CTGCAG	1 cut(s)	235
BspMI	ACCTGC	1 cut(s)	1084
BspT107I	GGYRCC	1 cut(s)	988
BsrDI	GCAATG	1 cut(s)	694
BsrFI	RCCGGY	1 cut(s)	161
BsrI	ACTGG	5 cut(s)	10, 1147, 1214, 1974, 2094
BssAI	RCCGGY	1 cut(s)	161
BssECI	CCNNGG	4 cut(s)	93, 1100, 1484, 1524
BssNI	GRCGYC	1 cut(s)	153
BssSI	CACGAG	2 cut(s)	268, 1317
BssT1I	CCWWGG	2 cut(s)	93, 1100
Bst2BI	CACGAG	2 cut(s)	268, 1317
Bst2UI	CCWGG	5 cut(s)	534, 1266, 1278, 1485, 1526
Bst4CI	ACNGT	6 cut(s)	337, 388, 734, 1211, 2129, 2150
Bst6I	CTCTTC	5 cut(s)	149, 1485, 1790, 1896, 1945
BstACI	GRCGYC	1 cut(s)	153
BstAPI	GCANNNNNTGC	4 cut(s)	326, 1040, 1253, 1500
BstC8I	GCNNGC	5 cut(s)	480, 651, 728, 846, 1776
BstDEI	CTNAG	6 cut(s)	330, 839, 1707, 1861, 2065, 2142
BstENI	CCTNNNNNAGG	2 cut(s)	1005, 1482
BstF5I	GGATG	5 cut(s)	424, 497, 1231, 1467, 2215
BstFNI	CGCG	3 cut(s)	81, 957, 1804
BstMAI	GTCTC	5 cut(s)	326, 957, 1133, 1200, 1624
BstMCI	CGRYCG	1 cut(s)	61
BstNI	CCWGG	5 cut(s)	534, 1266, 1278, 1485, 1526
BstNSI	RCATGY	4 cut(s)	248, 653, 1180, 1417
BstSCI	CCNGG	5 cut(s)	532, 1264, 1276, 1483, 1524
BstSFI	CTRYAG	2 cut(s)	231, 1188
BstSLI	GKGCMC	1 cut(s)	275
BstUI	CGCG	3 cut(s)	81, 957, 1804
BstV1I	GCAGC	4 cut(s)	332, 710, 810, 2070
BstV2I	GAAGAC	1 cut(s)	1617
BstX2I	RGATCY	6 cut(s)	250, 406, 1016, 1528, 1670, 2069
BstXI	CCANNNNNNTGG	1 cut(s)	1296
BstYI	RGATCY	6 cut(s)	250, 406, 1016, 1528, 1670, 2069
BstZI	CGGCCG	1 cut(s)	58
Bsu15I	ATCGAT	1 cut(s)	1990
BsuI	GTATCC	2 cut(s)	378, 1445
BsuRI	GGCC	2 cut(s)	60, 1096
BsuTUI	ATCGAT	1 cut(s)	1990
BtgZI	GCGATG	2 cut(s)	1370, 1826
BtrI	CACGTC	1 cut(s)	364
BtsCI	GGATG	5 cut(s)	424, 497, 1231, 1467, 2215
BtsI	GCAGTG	4 cut(s)	631, 882, 1964, 1972
BtsIMutI	CAGTG	8 cut(s)	631, 882, 1221, 1796, 1964, 1972, 2101, 2155
BtuMI	TCGCGA	1 cut(s)	81
BveI	ACCTGC	1 cut(s)	1084
Cac8I	GCNNGC	5 cut(s)	480, 651, 728, 846, 1776
CaiI	CAGNNNCTG	2 cut(s)	1040, 2072
CciI	TCATGA	2 cut(s)	1591, 2179
Cfr10I	RCCGGY	1 cut(s)	161
Cfr13I	GGNCC	2 cut(s)	1381, 1728
ClaI	ATCGAT	1 cut(s)	1990
CseI	GACGC	1 cut(s)	161
Csp6I	GTAC	4 cut(s)	172, 242, 1167, 1286
CspAI	ACCGGT	1 cut(s)	161
CspCI	CAANNNNNGTGG	2 cut(s)	1655, 1690
CviQI	GTAC	4 cut(s)	172, 242, 1167, 1286
DdeI	CTNAG	6 cut(s)	330, 839, 1707, 1861, 2065, 2142
DraI	TTTAAA	1 cut(s)	1623
DraIII	CACNNNGTG	2 cut(s)	551, 1319
EaeI	YGGCCR	1 cut(s)	58
EagI	CGGCCG	1 cut(s)	58
Eam1104I	CTCTTC	5 cut(s)	149, 1485, 1790, 1896, 1945
EarI	CTCTTC	5 cut(s)	149, 1485, 1790, 1896, 1945
EciI	GGCGGA	1 cut(s)	2188
Ecl136II	GAGCTC	3 cut(s)	1307, 1826, 1929
EclXI	CGGCCG	1 cut(s)	58
Eco130I	CCWWGG	2 cut(s)	93, 1100
Eco24I	GRGCYC	4 cut(s)	480, 1309, 1828, 1931
Eco47I	GGWCC	2 cut(s)	1381, 1728
Eco52I	CGGCCG	1 cut(s)	58
Eco53kI	GAGCTC	3 cut(s)	1307, 1826, 1929
Eco57I	CTGAAG	2 cut(s)	612, 1095
EcoICRI	GAGCTC	3 cut(s)	1307, 1826, 1929
EcoNI	CCTNNNNNAGG	2 cut(s)	1005, 1482
EcoO109I	RGGNCCY	1 cut(s)	1728
EcoRI	GAATTC	1 cut(s)	933
EcoRII	CCWGG	5 cut(s)	532, 1264, 1276, 1483, 1524
EcoT14I	CCWWGG	2 cut(s)	93, 1100
EcoT22I	ATGCAT	2 cut(s)	694, 752
EcoT38I	GRGCYC	4 cut(s)	480, 1309, 1828, 1931
ErhI	CCWWGG	2 cut(s)	93, 1100
FalI	AAGNNNNNCTT	6 cut(s)	407, 439, 1084, 1116, 1118, 1150
FaqI	GGGAC	6 cut(s)	768, 1024, 1367, 1501, 1660, 1714
FauI	CCCGC	1 cut(s)	487
FauNDI	CATATG	1 cut(s)	1342
FbaI	TGATCA	1 cut(s)	1984
FblI	GTMKAC	1 cut(s)	300
Fnu4HI	GCNGC	5 cut(s)	321, 724, 824, 958, 2059
FokI	GGATG	5 cut(s)	431, 504, 1218, 1474, 2222
FriOI	GRGCYC	4 cut(s)	480, 1309, 1828, 1931
Fsp4HI	GCNGC	5 cut(s)	321, 724, 824, 958, 2059
FspBI	CTAG	6 cut(s)	638, 863, 1491, 2133, 2241, 2260
GluI	GCNGC	5 cut(s)	321, 724, 824, 958, 2059
GsuI	CTGGAG	3 cut(s)	873, 900, 1915
HaeIII	GGCC	2 cut(s)	60, 1096
HapII	CCGG	1 cut(s)	162
HgaI	GACGC	1 cut(s)	161
Hin1I	GRCGYC	1 cut(s)	153
HincII	GTYRAC	5 cut(s)	34, 301, 340, 361, 663
HindII	GTYRAC	5 cut(s)	34, 301, 340, 361, 663
HpaII	CCGG	1 cut(s)	162
HphI	GGTGA	1 cut(s)	1305
Hpy166II	GTNNAC	5 cut(s)	34, 301, 340, 361, 663
Hpy8I	GTNNAC	5 cut(s)	34, 301, 340, 361, 663
Hpy99I	CGWCG	4 cut(s)	50, 59, 182, 305
HpyAV	CCTTC	4 cut(s)	1109, 1449, 1706, 2219
HpyCH4III	ACNGT	6 cut(s)	337, 388, 734, 1211, 2129, 2150
HpyCH4IV	ACGT	2 cut(s)	363, 1165
HpyF3I	CTNAG	6 cut(s)	330, 839, 1707, 1861, 2065, 2142
HpySE526I	ACGT	2 cut(s)	363, 1165
Hsp92I	GRCGYC	1 cut(s)	153
KflI	GGGWCCC	1 cut(s)	1728
Ksp22I	TGATCA	1 cut(s)	1984
Lsp1109I	GCAGC	4 cut(s)	332, 710, 810, 2070
LweI	GCATC	3 cut(s)	468, 482, 514
MaeI	CTAG	6 cut(s)	638, 863, 1491, 2133, 2241, 2260
MaeII	ACGT	2 cut(s)	363, 1165
MaeIII	GTNAC	6 cut(s)	157, 1121, 1172, 1598, 1700, 2123
MfeI	CAATTG	1 cut(s)	849
MflI	RGATCY	6 cut(s)	250, 406, 1016, 1528, 1670, 2069
MhlI	GDGCHC	5 cut(s)	275, 480, 1309, 1828, 1931
MlyI	GAGTC	4 cut(s)	125, 287, 2053, 2185
MmeI	TCCRAC	2 cut(s)	1958, 2187
Mph1103I	ATGCAT	2 cut(s)	694, 752
MroXI	GAANNNNTTC	1 cut(s)	1558
MseI	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
MslI	CAYNNNNRTG	5 cut(s)	608, 1460, 1596, 1638, 1761
MspA1I	CMGCKG	1 cut(s)	482
MspI	CCGG	1 cut(s)	162
MspR9I	CCNGG	5 cut(s)	534, 1266, 1278, 1485, 1526
MunI	CAATTG	1 cut(s)	849
Mva1269I	GAATGC	1 cut(s)	331
MvaI	CCWGG	5 cut(s)	534, 1266, 1278, 1485, 1526
MvnI	CGCG	3 cut(s)	81, 957, 1804
NdeI	CATATG	1 cut(s)	1342
NlaIV	GGNNCC	8 cut(s)	408, 477, 990, 1270, 1383, 1729, 1730, 2092
NmeAIII	GCCGAG	1 cut(s)	86
NmuCI	GTSAC	3 cut(s)	1121, 1598, 2123
NruI	TCGCGA	1 cut(s)	81
NsiI	ATGCAT	2 cut(s)	694, 752
NspI	RCATGY	4 cut(s)	248, 653, 1180, 1417
PaeI	GCATGC	1 cut(s)	653
PagI	TCATGA	2 cut(s)	1591, 2179
PciI	ACATGT	2 cut(s)	244, 1176
PctI	GAATGC	1 cut(s)	331
PdmI	GAANNNNTTC	1 cut(s)	1558
PfeI	GAWTC	9 cut(s)	41, 277, 631, 866, 926, 1037, 1520, 1970, 2030
PflFI	GACNNNGTC	1 cut(s)	296
PfoI	TCCNGGA	2 cut(s)	532, 1264
PinAI	ACCGGT	1 cut(s)	161
PkrI	GCNGC	5 cut(s)	322, 725, 825, 959, 2060
PleI	GAGTC	4 cut(s)	125, 287, 2052, 2184
PpsI	GAGTC	4 cut(s)	125, 287, 2052, 2184
PpuMI	RGGWCCY	1 cut(s)	1728
PscI	ACATGT	2 cut(s)	244, 1176
Psp124BI	GAGCTC	3 cut(s)	1309, 1828, 1931
Psp5II	RGGWCCY	1 cut(s)	1728
Psp6I	CCWGG	5 cut(s)	532, 1264, 1276, 1483, 1524
PspGI	CCWGG	5 cut(s)	532, 1264, 1276, 1483, 1524
PspN4I	GGNNCC	8 cut(s)	408, 477, 990, 1270, 1383, 1729, 1730, 2092
PspPI	GGNCC	2 cut(s)	1381, 1728
PspPPI	RGGWCCY	1 cut(s)	1728
PstI	CTGCAG	1 cut(s)	235
PstNI	CAGNNNCTG	2 cut(s)	1040, 2072
PsuI	RGATCY	6 cut(s)	250, 406, 1016, 1528, 1670, 2069
PsyI	GACNNNGTC	1 cut(s)	296
RruI	TCGCGA	1 cut(s)	81
RsaI	GTAC	4 cut(s)	173, 243, 1168, 1287
RsaNI	GTAC	4 cut(s)	172, 242, 1167, 1286
RseI	CAYNNNNRTG	5 cut(s)	608, 1460, 1596, 1638, 1761
SacI	GAGCTC	3 cut(s)	1309, 1828, 1931
SalI	GTCGAC	1 cut(s)	299
SaqAI	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
SatI	GCNGC	5 cut(s)	321, 724, 824, 958, 2059
Sau96I	GGNCC	2 cut(s)	1381, 1728
SbfI	CCTGCAGG	1 cut(s)	235
SchI	GAGTC	4 cut(s)	125, 287, 2053, 2185
ScrFI	CCNGG	5 cut(s)	534, 1266, 1278, 1485, 1526
SdaI	CCTGCAGG	1 cut(s)	235
SduI	GDGCHC	5 cut(s)	275, 480, 1309, 1828, 1931
SfaNI	GCATC	3 cut(s)	468, 482, 514
SfcI	CTRYAG	2 cut(s)	231, 1188
SinI	GGWCC	2 cut(s)	1381, 1728
SmiMI	CAYNNNNRTG	5 cut(s)	608, 1460, 1596, 1638, 1761
SmlI	CTYRAG	1 cut(s)	756
SmoI	CTYRAG	1 cut(s)	756
SphI	GCATGC	1 cut(s)	653
Sse8387I	CCTGCAGG	1 cut(s)	235
SsiI	CCGC	3 cut(s)	480, 957, 2173
SspI	AATATT	2 cut(s)	187, 1260
SspMI	CTAG	6 cut(s)	638, 863, 1491, 2133, 2241, 2260
SstI	GAGCTC	3 cut(s)	1309, 1828, 1931
StyD4I	CCNGG	5 cut(s)	532, 1264, 1276, 1483, 1524
StyI	CCWWGG	2 cut(s)	93, 1100
TaaI	ACNGT	6 cut(s)	337, 388, 734, 1211, 2129, 2150
TaiI	ACGT	2 cut(s)	366, 1168
TaqI	TCGA	7 cut(s)	39, 54, 300, 797, 1948, 1990, 2022
TatI	WGTACW	2 cut(s)	171, 241
TauI	GCSGC	1 cut(s)	960
TfiI	GAWTC	9 cut(s)	41, 277, 631, 866, 926, 1037, 1520, 1970, 2030
Tru1I	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
Tru9I	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
TscAI	CASTG	8 cut(s)	631, 889, 1221, 1796, 1964, 1972, 2101, 2155
TseFI	GTSAC	3 cut(s)	1121, 1598, 2123
TseI	GCWGC	4 cut(s)	320, 723, 823, 2058
Tsp45I	GTSAC	3 cut(s)	1121, 1598, 2123
TspGWI	ACGGA	5 cut(s)	19, 34, 64, 186, 1862
TspRI	CASTG	8 cut(s)	631, 889, 1221, 1796, 1964, 1972, 2101, 2155
Tth111I	GACNNNGTC	1 cut(s)	296
VpaK11BI	GGWCC	2 cut(s)	1381, 1728
XagI	CCTNNNNNAGG	2 cut(s)	1005, 1482
XapI	RAATTY	5 cut(s)	17, 192, 874, 933, 1570
XbaI	TCTAGA	2 cut(s)	2132, 2240
XceI	RCATGY	4 cut(s)	248, 653, 1180, 1417
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2069
XmiI	GTMKAC	1 cut(s)	300
XmnI	GAANNNNTTC	1 cut(s)	1558
XspI	CTAG	6 cut(s)	638, 863, 1491, 2133, 2241, 2260
Zsp2I	ATGCAT	2 cut(s)	694, 752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.