Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0009656

Physical Location & Seq

Genomic Coordinates Forward (+)

1227066 .. 1231393

Gene Structure 4328 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010383

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGGCGGTTCAGTTCAAATTCCGAAGCTCTGCGAAATTCGATTCTGTCGACCTCGAAGGTCGAGCCTCAATCCCCGTTCGTGATCTCAAAGCCAAAATCATCCGCCACAACAACCTCAACATCTGTCAAGATTCCGCTCTCGTCTTCTCCGACGCCATAACCGGTCAAGAGTACACTAACGAGAATATTAAAATTCCGTCCGGCTCGTCCGTGATTATCAAGAGAGTTCCTGCAGGATCGGCGGTACATGTGGATATGCCTCACTTCAACTGCAAGGAAGCTGGGAATCCGCCGAATGTTGAGACAGTCGACTTTGATGACTTTGGGGTTGACGTGTATCCCGTTCCCGAGGCAACCTTCGTCTGTTCTGGTTTGGATGCAGGCAAGGATGCCTGCAGTCCGGGTTATGAACCAAATACTGGCATCACAAGATGTTTGGAACCAGCAGTAGGAGGATGTCAGAAACTTGAAGCAAGTGGGATTAGTGATGCTATTCCAGCAAGTCGTTCGCATGGTGGAGTTGAACAAATCTTACCACAGACAAAGTCAAAGCCTGAGGTTCAAGAAGACTTTAATTTGGAAAAGATGGGTTGTGATCCCGTAGATACAAAACATGCTGATATGCCATCAGAGATGACATGCTCTCTATGCAACTCGTTTTTCAAGGAGGCTGTGATGATACCCTGCTGCCAGCACAGTTTTTGTGAGAAATGTATACATCAAATGCTACTTGAGAGGGCAAGATGTCCCAAGTGTTTTTCAACCAAGTGCAGAATTGAAGATCTGCTACCAAATATATCTCTTAGGCAAGCAATGGACCATTTCCAAGAGTCTCAAAATTTAATCACTGTTCCAGATAATGCTTTTTGCCAATACGCTCCAGATGGAGAATCGGGAATTCAAGCAAAAGATGTCTCTTGTGGTGGTAGTATTTTTCAAAGGGAGTCAGAGTTGCATCACTCTCCTGAAACAGGGATGGGATCTAATAATTGCTTAGCAGAATCTGCTTGTCACTCACCATTCAAAAATGATGTGCCGTATCATTTTGGTGCAAGAAACATGCAAAAAATTCCCAAGTTTTCGCACAAGAACAATCAAATCGATGGAGATGCATACGGTTCTACTCGTGTGGCTCCATATGACTCGGCAGCTTTTGATGACTTCCAAGGGGAAAGCAAGCCTATACCTGAGGAAGCTGAACCTGTAATCAAGAAAAAGAGGCCACCTTGGGTTTATACTGCAGGTGGTGGAAAAAGTTTTTTGGAGAGTGGGAGAAATAGAAAGGGGGATCGTGCCTGTTATATGTGTGGCTCCCCAGACCATTTAATAAGAGTGTGTCCAGTTGCTACCAGTCCACGCCCTATGCTTCATTCAGGAAATGCAGTCTTTCCAGGAGCTATGCCAGGTTATGTGCCACCATACTGGGATGGGGCTCTGTTGCCTCATACCCCGCCCTTTAGAAATCTATACGGAAATCATGGGATGACTGCTTTTGACACCATGTTGATCCCTCACGCTCCTTTTTCTGTTCATCCATACGTGCACACACCGTCCATGTATAGATCGATTCCAGGCTTTGGTGGATATATGGGAATGGGAGGTGTAGCACCTCTTCCTGAAACTGGTGAAGACTGTTATATAAGTCGTTCAGAATTTCTGGATCTTGAAGGTCAAGAAAAGAGGAGAAAACTTTCAAAGGAGGATTTTAGGAGAGATCAATCTTGTGATGACGATGAAGACTGGGGGAGGCATCAGAATAATGGGAGTGCAAGATTGCAAGAGCATAAATCTTTTGTAAACAGGGAAAAGAGTGTAAGCTACTCCAGTGACGGCTCTACTAAAAGGTCTCAGAGACAAGATCGGCATCGTAATCAGATAGATGATGACAACATTCATTTGGTTGGTCATCGGAAGAGTTCTCATTTGGTGGTTGGTCATCAGGACGGGAAACTGTATAATCGCACCGACAAATCAAGTTCAGAATTAGATGATATGCCAAGTAGCTCCAGTTGGCACAGTGAAGAGAAACACAAAAATTATCATAGAAGCTCCAGAAGGCAAAATGAAAGAAGAGAGCAGTGTGACAGTGATAAACACAAACACTATCATAGAAGCTCCAGATATCAAAAGGAAAGAAGAGAGCAGTGTGGTAGTGATTCTAGTCGGAGTCATCATCAAAATATCATTGAGAAAGATGATAAGAGAAAGGGAATTCAGCATAATAACAAAAGGCACAAACATCACAGTAACTCGGGATCTGGTTTAGAACACAGTGTTTCCAGTGATAAGAAGCTACAGAAAGGGAGCAGTCACAATTTTAGAAAATCTGAGCACAATGCAAAGTCTAGTGTTGAAGAGCGCAGTCATGACAGATGGAAAATGGTTAGTGGGTCAGATGAAGATAGTGGAGAAGATTACCAGTACTGTAAACGGAAAAGGGTAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001701 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006275 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009792 GO:0009888 GO:0009889 GO:0009987 GO:0010556 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0035264 GO:0036211 GO:0040007 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048513 GO:0048568 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051603 GO:0051716 GO:0060255 GO:0060429 GO:0061053 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DWNN zf-C3HC4_2 zf-CCHC

Protein Analysis

816

Amino Acids

91.47

Weight (kDa)

7.12

Isoelectric Point (pI)

61.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DWNN	PF08783	3 - 77	3.8e-18	DWNN domain
zf-C3HC4_2	PF13923	214 - 252	4.7e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120
malus_domestica	MD08G1070600.v1.1 MD15G1051500.v1.1
prunus_persica	Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1
pyrus_communis	pycom08g05840 pycom15g04930
rosa_chinensis	RchiOBHm_Chr6g0311661
rosa_laevigata	RLG00000010383
rosa_roxburghii	Rroxscaffold_7G00157400
rosa_rugosa	Rorug06G0390500.1
rosa_samantha	Rh6AG505800 Rh6BG516600 Rh6CG522500 Rh6DG508200
rosa_wichuraiana	Rw6G044020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1232
Acc36I	ACCTGC	1 cut(s)	1232
AccB7I	CCANNNNNTGG	2 cut(s)	419, 1577
AccBSI	CCGCTC	1 cut(s)	137
AccI	GTMKAC	3 cut(s)	48, 309, 715
AciI	CCGC	6 cut(s)	5, 103, 135, 242, 290, 1451
AclWI	GGATC	7 cut(s)	244, 590, 988, 1296, 1501, 1668, 2263
AcsI	RAATTY	8 cut(s)	17, 35, 192, 838, 897, 1068, 1652, 2211
AcyI	GRCGYC	1 cut(s)	153
AfaI	GTAC	3 cut(s)	173, 246, 2423
AfiI	CCNNNNNNNGG	5 cut(s)	419, 449, 971, 1577, 1622
AflIII	ACRYGT	2 cut(s)	247, 333
AgeI	ACCGGT	1 cut(s)	161
AjiI	CACGTC	1 cut(s)	334
AjnI	CCWGG	3 cut(s)	1390, 1402, 1570
Alw21I	GWGCWC	2 cut(s)	1545, 2334
Alw26I	GTCTC	5 cut(s)	296, 837, 919, 1846, 1851
Alw44I	GTGCAC	1 cut(s)	1541
AlwI	GGATC	7 cut(s)	244, 590, 988, 1296, 1501, 1668, 2263
AlwNI	CAGNNNCTG	1 cut(s)	1004
Ama87I	CYCGRG	2 cut(s)	347, 2251
AoxI	GGCC	1 cut(s)	1220
ApaLI	GTGCAC	1 cut(s)	1541
ApeKI	GCWGC	2 cut(s)	687, 1148
ApoI	RAATTY	8 cut(s)	17, 35, 192, 838, 897, 1068, 1652, 2211
AsiGI	ACCGGT	1 cut(s)	161
Asp700I	GAANNNNTTC	1 cut(s)	1690
AspLEI	GCGC	1 cut(s)	2361
AspS9I	GGNCC	1 cut(s)	817
AsuC2I	CCSGG	1 cut(s)	402
AsuHPI	GGTGA	2 cut(s)	1008, 1637
AvaI	CYCGRG	2 cut(s)	347, 2251
AvaII	GGWCC	1 cut(s)	817
AxyI	CCTNAGG	2 cut(s)	555, 1188
BaeGI	GKGCMC	1 cut(s)	1545
BanII	GRGCYC	1 cut(s)	1435
BarI	GAAGNNNNNNTAC	4 cut(s)	771, 803, 1596, 1628
BauI	CACGAG	1 cut(s)	1125
BbsI	GAAGAC	4 cut(s)	136, 573, 1635, 1743
Bbv12I	GWGCWC	2 cut(s)	1545, 2334
BbvI	GCAGC	2 cut(s)	674, 1160
BccI	CCATC	7 cut(s)	580, 634, 878, 970, 1097, 1421, 2367
BceAI	ACGGC	2 cut(s)	1021, 1846
BcgI	CGANNNNNNTGC	4 cut(s)	489, 523, 1091, 1125
BciT130I	CCWGG	3 cut(s)	1392, 1404, 1572
BciVI	GTATCC	1 cut(s)	348
BcnI	CCSGG	1 cut(s)	402
BcoDI	GTCTC	5 cut(s)	296, 837, 919, 1846, 1851
BfaI	CTAG	2 cut(s)	2160, 2346
BfmI	CTRYAG	4 cut(s)	231, 394, 1239, 2294
BfuAI	ACCTGC	1 cut(s)	1232
BfuI	GTATCC	1 cut(s)	348
BglII	AGATCT	1 cut(s)	781
BisI	GCNGC	2 cut(s)	688, 1149
BlpI	GCTNAGC	1 cut(s)	994
BlsI	GCNGC	2 cut(s)	689, 1150
BmcAI	AGTACT	1 cut(s)	2423
Bme1390I	CCNGG	4 cut(s)	402, 1392, 1404, 1572
Bme18I	GGWCC	1 cut(s)	817
BmeT110I	CYCGRG	2 cut(s)	347, 2251
BmgBI	CACGTC	1 cut(s)	334
BmgT120I	GGNCC	1 cut(s)	817
BmiI	GGNNCC	3 cut(s)	441, 1134, 1312
BmrFI	CCNGG	4 cut(s)	402, 1392, 1404, 1572
BmrI	ACTGGG	2 cut(s)	1432, 1750
BmsI	GCATC	8 cut(s)	367, 379, 432, 478, 964, 1099, 1759, 1873
BmuI	ACTGGG	2 cut(s)	1432, 1750
BpiI	GAAGAC	4 cut(s)	136, 573, 1635, 1743
BpmI	CTGGAG	5 cut(s)	864, 1807, 1990, 2035, 2101
Bpu1102I	GCTNAGC	1 cut(s)	994
BpuEI	CTTGAG	1 cut(s)	752
BpuMI	CCSGG	1 cut(s)	402
Bsa29I	ATCGAT	2 cut(s)	1101, 1565
BsaAI	YACGTR	1 cut(s)	1540
BsaBI	GATNNNNATC	1 cut(s)	1863
BsaHI	GRCGYC	1 cut(s)	153
BsaI	GGTCTC	1 cut(s)	1851
BsaJI	CCNNGG	3 cut(s)	348, 1165, 1226
BsaWI	WCCGGW	1 cut(s)	161
BsaXI	ACNNNNNCTCC	2 cut(s)	935, 965
Bsc4I	CCNNNNNNNGG	5 cut(s)	419, 449, 971, 1577, 1622
Bse118I	RCCGGY	1 cut(s)	161
Bse21I	CCTNAGG	2 cut(s)	555, 1188
Bse3DI	GCAATG	1 cut(s)	819
Bse8I	GATNNNNATC	1 cut(s)	1863
BseBI	CCWGG	3 cut(s)	1392, 1404, 1572
BseCI	ATCGAT	2 cut(s)	1101, 1565
BseDI	CCNNGG	3 cut(s)	348, 1165, 1226
BseGI	GGATG	8 cut(s)	99, 382, 394, 461, 981, 1432, 1488, 1531
BseJI	GATNNNNATC	1 cut(s)	1863
BseLI	CCNNNNNNNGG	5 cut(s)	419, 449, 971, 1577, 1622
BseMI	GCAATG	1 cut(s)	819
BseMII	CTCAG	4 cut(s)	546, 1179, 1862, 2319
BseRI	GAGGAG	1 cut(s)	1696
BseSI	GKGCMC	1 cut(s)	1545
BseXI	GCAGC	2 cut(s)	674, 1160
BseYI	CCCAGC	1 cut(s)	281
BsgI	GTGCAG	1 cut(s)	790
BshFI	GGCC	1 cut(s)	1222
BshTI	ACCGGT	1 cut(s)	161
BshVI	ATCGAT	2 cut(s)	1101, 1565
BsiHKAI	GWGCWC	2 cut(s)	1545, 2334
BsiHKCI	CYCGRG	2 cut(s)	347, 2251
BsiSI	CCGG	3 cut(s)	162, 201, 401
BslFI	GGGAC	1 cut(s)	732
BslI	CCNNNNNNNGG	5 cut(s)	419, 449, 971, 1577, 1622
BsmAI	GTCTC	5 cut(s)	296, 837, 919, 1846, 1851
BsmFI	GGGAC	1 cut(s)	732
BsnI	GGCC	1 cut(s)	1222
Bso31I	GGTCTC	1 cut(s)	1851
BsoBI	CYCGRG	2 cut(s)	347, 2251
Bsp1286I	GDGCHC	3 cut(s)	1435, 1545, 2334
Bsp1720I	GCTNAGC	1 cut(s)	994
BspACI	CCGC	6 cut(s)	5, 103, 135, 242, 290, 1451
BspANI	GGCC	1 cut(s)	1222
BspCNI	CTCAG	4 cut(s)	547, 1180, 1861, 2320
BspDI	ATCGAT	2 cut(s)	1101, 1565
BspHI	TCATGA	1 cut(s)	2365
BspLI	GGNNCC	3 cut(s)	441, 1134, 1312
BspMAI	CTGCAG	3 cut(s)	235, 398, 1243
BspMI	ACCTGC	1 cut(s)	1232
BspPI	GGATC	7 cut(s)	244, 590, 988, 1296, 1501, 1668, 2263
BspQI	GCTCTTC	1 cut(s)	2349
BspTNI	GGTCTC	1 cut(s)	1851
BsrBI	CCGCTC	1 cut(s)	137
BsrDI	GCAATG	1 cut(s)	819
BsrFI	RCCGGY	1 cut(s)	161
BssAI	RCCGGY	1 cut(s)	161
BssECI	CCNNGG	3 cut(s)	348, 1165, 1226
BssNAI	GTATAC	1 cut(s)	716
BssNI	GRCGYC	1 cut(s)	153
BssSI	CACGAG	1 cut(s)	1125
BssT1I	CCWWGG	2 cut(s)	1165, 1226
Bst1107I	GTATAC	1 cut(s)	716
Bst2BI	CACGAG	1 cut(s)	1125
Bst2UI	CCWGG	3 cut(s)	1392, 1404, 1572
Bst6I	CTCTTC	6 cut(s)	1617, 1907, 2016, 2065, 2131, 2349
BstACI	GRCGYC	1 cut(s)	153
BstAPI	GCANNNNNTGC	1 cut(s)	1004
BstBAI	YACGTR	1 cut(s)	1540
BstC8I	GCNNGC	5 cut(s)	382, 394, 692, 810, 1178
BstDEI	CTNAG	6 cut(s)	555, 803, 994, 1188, 1848, 2328
BstENI	CCTNNNNNAGG	1 cut(s)	969
BstF5I	GGATG	8 cut(s)	99, 382, 394, 461, 981, 1432, 1488, 1531
BstHHI	GCGC	1 cut(s)	2361
BstMAI	GTCTC	5 cut(s)	296, 837, 919, 1846, 1851
BstMWI	GCNNNNNNNGC	4 cut(s)	239, 497, 648, 1004
BstNI	CCWGG	3 cut(s)	1392, 1404, 1572
BstNSI	RCATGY	4 cut(s)	251, 617, 642, 1063
BstSCI	CCNGG	4 cut(s)	400, 1390, 1402, 1570
BstSFI	CTRYAG	4 cut(s)	231, 394, 1239, 2294
BstSLI	GKGCMC	1 cut(s)	1545
BstV1I	GCAGC	2 cut(s)	674, 1160
BstV2I	GAAGAC	4 cut(s)	136, 573, 1635, 1743
BstX2I	RGATCY	4 cut(s)	781, 980, 1660, 2255
BstXI	CCANNNNNNTGG	1 cut(s)	1422
BstYI	RGATCY	4 cut(s)	781, 980, 1660, 2255
BstZ17I	GTATAC	1 cut(s)	716
Bsu15I	ATCGAT	2 cut(s)	1101, 1565
Bsu36I	CCTNAGG	2 cut(s)	555, 1188
BsuI	GTATCC	1 cut(s)	348
BsuRI	GGCC	1 cut(s)	1222
BsuTUI	ATCGAT	2 cut(s)	1101, 1565
BtrI	CACGTC	1 cut(s)	334
BtsCI	GGATG	8 cut(s)	99, 382, 394, 461, 981, 1432, 1488, 1531
BtsI	GCAGTG	2 cut(s)	2084, 2150
BtsIMutI	CAGTG	8 cut(s)	846, 1831, 2023, 2084, 2092, 2150, 2278, 2287
BveI	ACCTGC	1 cut(s)	1232
Cac8I	GCNNGC	5 cut(s)	382, 394, 692, 810, 1178
CaiI	CAGNNNCTG	1 cut(s)	1004
CciI	TCATGA	1 cut(s)	2365
CfoI	GCGC	1 cut(s)	2361
Cfr10I	RCCGGY	1 cut(s)	161
Cfr13I	GGNCC	1 cut(s)	817
ClaI	ATCGAT	2 cut(s)	1101, 1565
CseI	GACGC	1 cut(s)	161
Csp6I	GTAC	3 cut(s)	172, 245, 2422
CspAI	ACCGGT	1 cut(s)	161
CviAII	CATG	9 cut(s)	248, 512, 614, 639, 1060, 1478, 1501, 1555, 2366
CviQI	GTAC	3 cut(s)	172, 245, 2422
DdeI	CTNAG	6 cut(s)	555, 803, 994, 1188, 1848, 2328
Eam1104I	CTCTTC	6 cut(s)	1617, 1907, 2016, 2065, 2131, 2349
EarI	CTCTTC	6 cut(s)	1617, 1907, 2016, 2065, 2131, 2349
EciI	GGCGGA	2 cut(s)	92, 279
Eco130I	CCWWGG	2 cut(s)	1165, 1226
Eco24I	GRGCYC	1 cut(s)	1435
Eco31I	GGTCTC	1 cut(s)	1851
Eco32I	GATATC	1 cut(s)	2123
Eco47I	GGWCC	1 cut(s)	817
Eco81I	CCTNAGG	2 cut(s)	555, 1188
Eco88I	CYCGRG	2 cut(s)	347, 2251
EcoNI	CCTNNNNNAGG	1 cut(s)	969
EcoRI	GAATTC	2 cut(s)	897, 2211
EcoRII	CCWGG	3 cut(s)	1390, 1402, 1570
EcoRV	GATATC	1 cut(s)	2123
EcoT14I	CCWWGG	2 cut(s)	1165, 1226
EcoT22I	ATGCAT	1 cut(s)	1114
EcoT38I	GRGCYC	1 cut(s)	1435
ErhI	CCWWGG	2 cut(s)	1165, 1226
FaeI	CATG	9 cut(s)	251, 515, 617, 642, 1063, 1481, 1504, 1558, 2369
FaqI	GGGAC	1 cut(s)	732
FatI	CATG	9 cut(s)	247, 511, 613, 638, 1059, 1477, 1500, 1554, 2365
FauI	CCCGC	1 cut(s)	1458
FauNDI	CATATG	1 cut(s)	1138
FblI	GTMKAC	3 cut(s)	48, 309, 715
Fnu4HI	GCNGC	2 cut(s)	688, 1149
FokI	GGATG	8 cut(s)	86, 389, 401, 468, 988, 1439, 1495, 1518
FriOI	GRGCYC	1 cut(s)	1435
Fsp4HI	GCNGC	2 cut(s)	688, 1149
FspBI	CTAG	2 cut(s)	2160, 2346
GlaI	GCGC	1 cut(s)	2360
GluI	GCNGC	2 cut(s)	688, 1149
GsaI	CCCAGC	1 cut(s)	285
GsuI	CTGGAG	5 cut(s)	864, 1807, 1990, 2035, 2101
HaeIII	GGCC	1 cut(s)	1222
HapII	CCGG	3 cut(s)	162, 201, 401
HgaI	GACGC	1 cut(s)	161
HhaI	GCGC	1 cut(s)	2361
Hin1I	GRCGYC	1 cut(s)	153
Hin1II	CATG	9 cut(s)	251, 515, 617, 642, 1063, 1481, 1504, 1558, 2369
Hin6I	GCGC	1 cut(s)	2359
HinP1I	GCGC	1 cut(s)	2359
HincII	GTYRAC	3 cut(s)	49, 310, 331
HindII	GTYRAC	3 cut(s)	49, 310, 331
HpaII	CCGG	3 cut(s)	162, 201, 401
HphI	GGTGA	2 cut(s)	1008, 1637
Hpy166II	GTNNAC	9 cut(s)	49, 174, 310, 331, 716, 1355, 1543, 1798, 2429
Hpy8I	GTNNAC	9 cut(s)	49, 174, 310, 331, 716, 1355, 1543, 1798, 2429
Hpy99I	CGWCG	1 cut(s)	155
HpyAV	CCTTC	4 cut(s)	50, 367, 1661, 2049
HpyCH4IV	ACGT	2 cut(s)	333, 1539
HpyF10VI	GCNNNNNNNGC	4 cut(s)	239, 497, 648, 1004
HpyF3I	CTNAG	6 cut(s)	555, 803, 994, 1188, 1848, 2328
HpySE526I	ACGT	2 cut(s)	333, 1539
Hsp92I	GRCGYC	1 cut(s)	153
Hsp92II	CATG	9 cut(s)	251, 515, 617, 642, 1063, 1481, 1504, 1558, 2369
HspAI	GCGC	1 cut(s)	2359
LguI	GCTCTTC	1 cut(s)	2349
LmnI	GCTCC	9 cut(s)	883, 1138, 1316, 1394, 1522, 2009, 2054, 2120, 2304
Lsp1109I	GCAGC	2 cut(s)	674, 1160
LweI	GCATC	8 cut(s)	367, 379, 432, 478, 964, 1099, 1759, 1873
MaeI	CTAG	2 cut(s)	2160, 2346
MaeII	ACGT	2 cut(s)	333, 1539
MaeIII	GTNAC	5 cut(s)	1010, 1826, 2081, 2246, 2309
MbiI	CCGCTC	1 cut(s)	137
MflI	RGATCY	4 cut(s)	781, 980, 1660, 2255
MhlI	GDGCHC	3 cut(s)	1435, 1545, 2334
MlyI	GAGTC	4 cut(s)	839, 953, 1136, 2176
MmeI	TCCRAC	2 cut(s)	174, 2144
Mph1103I	ATGCAT	1 cut(s)	1114
MroXI	GAANNNNTTC	1 cut(s)	1690
MseI	TTAA	4 cut(s)	189, 573, 842, 1325
MslI	CAYNNNNRTG	1 cut(s)	1047
MspI	CCGG	3 cut(s)	162, 201, 401
MspR9I	CCNGG	4 cut(s)	402, 1392, 1404, 1572
MvaI	CCWGG	3 cut(s)	1392, 1404, 1572
MwoI	GCNNNNNNNGC	4 cut(s)	239, 497, 648, 1004
NciI	CCSGG	1 cut(s)	402
NdeI	CATATG	1 cut(s)	1138
NlaIII	CATG	9 cut(s)	251, 515, 617, 642, 1063, 1481, 1504, 1558, 2369
NlaIV	GGNNCC	3 cut(s)	441, 1134, 1312
NmeAIII	GCCGAG	1 cut(s)	1124
NmuCI	GTSAC	4 cut(s)	1010, 1826, 2081, 2309
NsiI	ATGCAT	1 cut(s)	1114
NspI	RCATGY	4 cut(s)	251, 617, 642, 1063
PagI	TCATGA	1 cut(s)	2365
PaqCI	CACCTGC	1 cut(s)	1232
PciI	ACATGT	1 cut(s)	247
PciSI	GCTCTTC	1 cut(s)	2349
PcsI	WCGNNNNNNNCGW	3 cut(s)	45, 147, 339
PdmI	GAANNNNTTC	1 cut(s)	1690
PfeI	GAWTC	7 cut(s)	41, 131, 286, 890, 1001, 1567, 2156
PflFI	GACNNNGTC	1 cut(s)	544
PflMI	CCANNNNNTGG	2 cut(s)	419, 1577
PfoI	TCCNGGA	1 cut(s)	1390
PinAI	ACCGGT	1 cut(s)	161
PkrI	GCNGC	2 cut(s)	689, 1150
PleI	GAGTC	4 cut(s)	838, 952, 1136, 2175
PpsI	GAGTC	4 cut(s)	838, 952, 1136, 2175
Ppu21I	YACGTR	1 cut(s)	1540
PscI	ACATGT	1 cut(s)	247
Psp6I	CCWGG	3 cut(s)	1390, 1402, 1570
PspFI	CCCAGC	1 cut(s)	281
PspGI	CCWGG	3 cut(s)	1390, 1402, 1570
PspN4I	GGNNCC	3 cut(s)	441, 1134, 1312
PspPI	GGNCC	1 cut(s)	817
PsrI	GAACNNNNNNTAC	2 cut(s)	516, 548
PstI	CTGCAG	3 cut(s)	235, 398, 1243
PstNI	CAGNNNCTG	1 cut(s)	1004
PsuI	RGATCY	4 cut(s)	781, 980, 1660, 2255
PsyI	GACNNNGTC	1 cut(s)	544
RsaI	GTAC	3 cut(s)	173, 246, 2423
RsaNI	GTAC	3 cut(s)	172, 245, 2422
RseI	CAYNNNNRTG	1 cut(s)	1047
SalI	GTCGAC	2 cut(s)	47, 308
SapI	GCTCTTC	1 cut(s)	2349
SaqAI	TTAA	4 cut(s)	189, 573, 842, 1325
SatI	GCNGC	2 cut(s)	688, 1149
Sau96I	GGNCC	1 cut(s)	817
SbfI	CCTGCAGG	1 cut(s)	235
ScaI	AGTACT	1 cut(s)	2423
SchI	GAGTC	4 cut(s)	839, 953, 1136, 2176
ScrFI	CCNGG	4 cut(s)	402, 1392, 1404, 1572
SdaI	CCTGCAGG	1 cut(s)	235
SduI	GDGCHC	3 cut(s)	1435, 1545, 2334
SfaNI	GCATC	8 cut(s)	367, 379, 432, 478, 964, 1099, 1759, 1873
SfcI	CTRYAG	4 cut(s)	231, 394, 1239, 2294
SinI	GGWCC	1 cut(s)	817
SmiMI	CAYNNNNRTG	1 cut(s)	1047
SmlI	CTYRAG	1 cut(s)	731
SmoI	CTYRAG	1 cut(s)	731
Sse8387I	CCTGCAGG	1 cut(s)	235
SsiI	CCGC	6 cut(s)	5, 103, 135, 242, 290, 1451
SspI	AATATT	1 cut(s)	187
SspMI	CTAG	2 cut(s)	2160, 2346
StyD4I	CCNGG	4 cut(s)	400, 1390, 1402, 1570
StyI	CCWWGG	2 cut(s)	1165, 1226
TaiI	ACGT	2 cut(s)	336, 1542
TaqI	TCGA	7 cut(s)	39, 48, 54, 61, 309, 1101, 1565
TatI	WGTACW	2 cut(s)	171, 2421
TfiI	GAWTC	7 cut(s)	41, 131, 286, 890, 1001, 1567, 2156
Tru1I	TTAA	4 cut(s)	189, 573, 842, 1325
Tru9I	TTAA	4 cut(s)	189, 573, 842, 1325
TscAI	CASTG	8 cut(s)	853, 1831, 2023, 2084, 2092, 2150, 2278, 2287
TseFI	GTSAC	4 cut(s)	1010, 1826, 2081, 2309
TseI	GCWGC	2 cut(s)	687, 1148
Tsp45I	GTSAC	4 cut(s)	1010, 1826, 2081, 2309
TspDTI	ATGAA	7 cut(s)	423, 1358, 1520, 1749, 1883, 2079, 2412
TspGWI	ACGGA	4 cut(s)	186, 199, 1485, 2446
TspRI	CASTG	8 cut(s)	853, 1831, 2023, 2084, 2092, 2150, 2278, 2287
Tth111I	GACNNNGTC	1 cut(s)	544
Van91I	CCANNNNNTGG	2 cut(s)	419, 1577
VneI	GTGCAC	1 cut(s)	1541
VpaK11BI	GGWCC	1 cut(s)	817
XagI	CCTNNNNNAGG	1 cut(s)	969
XapI	RAATTY	8 cut(s)	17, 35, 192, 838, 897, 1068, 1652, 2211
XceI	RCATGY	4 cut(s)	251, 617, 642, 1063
XmiI	GTMKAC	3 cut(s)	48, 309, 715
XmnI	GAANNNNTTC	1 cut(s)	1690
XspI	CTAG	2 cut(s)	2160, 2346
ZrmI	AGTACT	1 cut(s)	2423
Zsp2I	ATGCAT	1 cut(s)	1114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.