Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0009656

Physical Location & Seq

Genomic Coordinates Reverse (-)

71036583 .. 71040933

Gene Structure 4351 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG516600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGTACGCTTTCGCACCAAGAAAACCGAAATTCCGTCTATTTTCCTCTCTCTATCTGACACCTTTGATCATCCGAGCTCTGACCCACCGATTCGACTCGGCCATGGCGGTTCAGTTCAAATTCCGAAGCTCTGCGAAATTCGATTCCGTCGACCTCGAAGGTCGAGCCTCAATCCCCGTTCGTGATCTCAAAGCCAAAATCATCCGCCACAACAACCTCAACATCTGTCAAGATTCCGATCTCGTCTTCTCCGACGCCATAACCGGTCAAGAGTACACTAACGAGAATATTCAAATTCCGTGCGGCTCGTCCGTGATTATCAAGAGAGTTCCTGCAGGATCGGCACATGTGGATATGCCTCACTTCAACTGCAAGGAAGCTGGGAATCCGCCGAATGTTGAGACAGTCGACTTTGATGACTTTGGGGTTGACGTGTATCCCGTTCCCGAGGCAACCTTCGTCTGTTCTGGTTTGGATGCAGACAAGGATGCCCGCAGTCGGGGATATGAACCAAATACTGGCATCACAAGATGTTTGGAGCCAGCAGTAGGAGGATGTCAGAAACTTGAAGCAAGTGGGATTAGTGATGCTATTCCAGGAAGTCATTCGCATGGTGGAGTTGAACAAATCTTACCACAGACAAACTCAAAGCCTGAGGTTCAAGAAGACTTTAATTTGGAAAAGATGGGTTGTGATCCCGTAGATACAAAACATGCTGATATGCCATCAGAGATGAAATGCTCTCTATGCAACTCGTTTTTCAAGGAGGCTGTGATGATACCCTGCTGCCAGCACAGTTTTTGTGAGAAATGTATACATCAAATGCTGCTTGAGAAGGCAAGATGTCCCAAGTGTTTTTCAACCAAGTGCAGAATTGAAGATCTGCTACCAAATATATCTCTTAGGCAAGCAATGGACCATTTCCAAGAGTCTCAAAATTTAATCACTGTTCCAGATAATGCTTTTTGCCAATACGCTCCAGATGGAGAATCGGGAATTCAGGCAAAAGATGTCTCTTGTGGTGGTAGTATTTTTCAAAGGGAGTCAGAGTTGCCTCACTCTCCTGAAACAGGGATGGAATCTAATAATTGCTTAGCAGAATCTGCTTGTCACTCACCATTCAAAAATGATGCGCCGTATCATTTTGGTGCAAGAAACATGCAAAAAATTCCCAAGTTTTCGCACAAGAACAATCAAATCGATGGAGATGGATACGGTTATACTTGTGTGGCTCCATATGGCTCGGCAGCTTTTGATGATTTCCAAGGGGAAAGCAAGCCTATACCTAAGGAAGCTGAACCTGTAATCAAGAAAAAGAGGCCACCTTGGGTTTATACTGCAGGTGGTGGAAAAAGTTTTTTGGAGAGTGGGAGAAATAGAAAGGGGGATCGTGCCTGTTATATGTGTGGCTCCCCAGACCATTTAATAAGAGTGTGTCCAGTTGCTACCAGTCCACGCCCTATGCTTCATTCAGGAAATGCAGTCTTTCCAGGAGCTATGCCAGGTTATGTGCCACCATACTGGGATGGGGCTCTGTTGCCTCATACCCCGCCCTTTAGAAATCTATACGGAAATCATGGGATGACAGCTTTTGACACGTTGATCCCTCACACTCCTTTTTCTGTTCATCCATACATGCACACGCCATCCATGTATAGATCGATTCCAGGCTTTGGTGGATATATGGGAATGGGAGGTGTAGCACCTCTTCCTGAAACTGGTGAAGACTGTTATATAAGTCGTTCAGAATTTCTGGATCTTGAAGGTCAAGAAAAGAGGAGAAAACTTTCAAGGGAGAATTTTAGGAGAGATCAATCTTGTGATGACGATGAAGACTGGGGGAGGCATCAGTATAATGGGAGTGCAAGATTGCAAGAGCATAAATCTTTTGTAAACAGGGAAAAGAGTGTAAGCTACTCCAGTGACGGCTCTACTAAAAGGTCTCAGAGACAAGATCGGCATCGTAATCAGATAGATGATGACAACATTCATTTGGTTGGTCATCGGAAGAGTTCTCATTTGGTGGTTGGTCATCAGGATGGGAAACTGTATAATCCCACGGAGAAATCAAGTTCGGAATTAGATGATATGCCAAGTAGCTCCAGTTGGCACAGTGAAGAGAAACACAAACATTATCATAGAAGCTCCAGAAAGCAAAATGAAAGAAGAGAGCAGTGTGACGGTGATAAACACAAACACTATCATAGAAGCTCCAGATATCACAAGGAAAGAAGAGAGCAGTGTGGTAGTGATTCTAGTCGGAGTCATCATCAAAATATCATTGAGAAAGATGATAAGAGAAAGGGAATTCAGCATAATAACAAAAGGCACAAACATCACAGTAACTCGGGATCTGATTTAGAACACAGTGTTTCCAGTGATAAGAAGCTACAGAAAGGGAGCAGTCACAATTTTAGAAAATCTGAGCACAATGCAAAGTCTAGTGTTGATGAGCGCAGTCATGAAAGATGGAAAATGGTTAGTGGGTCAGATGAAGATAGTGGAGAAGATTATCGGTACTGTAAACGGAAAAGGGTAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001701 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006275 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009792 GO:0009888 GO:0009889 GO:0009987 GO:0010556 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0035264 GO:0036211 GO:0040007 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048513 GO:0048568 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051603 GO:0051716 GO:0060255 GO:0060429 GO:0061053 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DWNN zf-C3HC4_2 zf-CCHC

Protein Analysis

847

Amino Acids

95.54

Weight (kDa)

7.74

Isoelectric Point (pI)

57.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DWNN	PF08783	37 - 111	2e-19	DWNN domain
zf-C3HC4_2	PF13923	246 - 285	1.9e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120
malus_domestica	MD08G1070600.v1.1 MD15G1051500.v1.1
prunus_persica	Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1
pyrus_communis	pycom08g05840 pycom15g04930
rosa_chinensis	RchiOBHm_Chr6g0311661
rosa_laevigata	RLG00000010383
rosa_roxburghii	Rroxscaffold_7G00157400
rosa_rugosa	Rorug06G0390500.1
rosa_samantha	Rh6AG505800 Rh6BG516600 Rh6CG522500 Rh6DG508200
rosa_wichuraiana	Rw6G044020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1331
Acc36I	ACCTGC	1 cut(s)	1331
AccB7I	CCANNNNNTGG	2 cut(s)	518, 1673
AccI	GTMKAC	3 cut(s)	150, 408, 814
AciI	CCGC	6 cut(s)	107, 205, 303, 389, 493, 1550
AclWI	GGATC	6 cut(s)	346, 689, 1395, 1597, 1764, 2359
AcoI	YGGCCR	1 cut(s)	99
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	3 cut(s)	5, 275, 2519
AfiI	CCNNNNNNNGG	7 cut(s)	498, 499, 518, 548, 1070, 1673, 1718
AflIII	ACRYGT	3 cut(s)	346, 432, 1596
AgeI	ACCGGT	1 cut(s)	263
AjiI	CACGTC	1 cut(s)	433
AjnI	CCWGG	4 cut(s)	595, 1489, 1501, 1666
Alw21I	GWGCWC	2 cut(s)	79, 2430
Alw26I	GTCTC	5 cut(s)	395, 936, 1018, 1942, 1947
AlwI	GGATC	6 cut(s)	346, 689, 1395, 1597, 1764, 2359
AlwNI	CAGNNNCTG	1 cut(s)	1103
Ama87I	CYCGRG	2 cut(s)	446, 2347
AoxI	GGCC	2 cut(s)	99, 1319
ApeKI	GCWGC	3 cut(s)	786, 826, 1247
AsiGI	ACCGGT	1 cut(s)	263
Asp700I	GAANNNNTTC	2 cut(s)	604, 1786
AspLEI	GCGC	2 cut(s)	1135, 2457
AspS9I	GGNCC	1 cut(s)	916
AsuHPI	GGTGA	3 cut(s)	1107, 1733, 2195
AvaI	CYCGRG	2 cut(s)	446, 2347
AvaII	GGWCC	1 cut(s)	916
AxyI	CCTNAGG	2 cut(s)	654, 1287
BanII	GRGCYC	2 cut(s)	79, 1534
BarI	GAAGNNNNNNTAC	4 cut(s)	870, 902, 1692, 1724
BbsI	GAAGAC	4 cut(s)	238, 672, 1731, 1839
Bbv12I	GWGCWC	2 cut(s)	79, 2430
BbvI	GCAGC	3 cut(s)	773, 813, 1259
BceAI	ACGGC	2 cut(s)	1120, 1942
BciT130I	CCWGG	4 cut(s)	597, 1491, 1503, 1668
BciVI	GTATCC	2 cut(s)	447, 1205
BclI	TGATCA	1 cut(s)	66
BcoDI	GTCTC	5 cut(s)	395, 936, 1018, 1942, 1947
BfaI	CTAG	2 cut(s)	2256, 2442
BfmI	CTRYAG	3 cut(s)	333, 1338, 2390
BfuAI	ACCTGC	1 cut(s)	1331
BfuI	GTATCC	2 cut(s)	447, 1205
BglII	AGATCT	1 cut(s)	880
BisI	GCNGC	4 cut(s)	304, 787, 827, 1248
BlpI	GCTNAGC	1 cut(s)	1093
BlsI	GCNGC	4 cut(s)	305, 788, 828, 1249
Bme1390I	CCNGG	4 cut(s)	597, 1491, 1503, 1668
Bme18I	GGWCC	1 cut(s)	916
BmeT110I	CYCGRG	2 cut(s)	446, 2347
BmgBI	CACGTC	1 cut(s)	433
BmgT120I	GGNCC	1 cut(s)	916
BmiI	GGNNCC	3 cut(s)	540, 1233, 1411
BmrFI	CCNGG	4 cut(s)	597, 1491, 1503, 1668
BmrI	ACTGGG	2 cut(s)	1531, 1846
BmsI	GCATC	7 cut(s)	466, 478, 531, 577, 1120, 1855, 1969
BmuI	ACTGGG	2 cut(s)	1531, 1846
BpiI	GAAGAC	4 cut(s)	238, 672, 1731, 1839
BpmI	CTGGAG	5 cut(s)	963, 1903, 2086, 2131, 2197
Bpu1102I	GCTNAGC	1 cut(s)	1093
BpuEI	CTTGAG	1 cut(s)	851
Bsa29I	ATCGAT	2 cut(s)	1200, 1661
BsaBI	GATNNNNATC	2 cut(s)	237, 1959
BsaHI	GRCGYC	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	1947
BsaJI	CCNNGG	5 cut(s)	102, 447, 1264, 1325, 2058
BsaWI	WCCGGW	1 cut(s)	263
BsaXI	ACNNNNNCTCC	2 cut(s)	1034, 1064
Bsc4I	CCNNNNNNNGG	7 cut(s)	498, 499, 518, 548, 1070, 1673, 1718
Bse118I	RCCGGY	1 cut(s)	263
Bse1I	ACTGG	9 cut(s)	523, 1439, 1449, 1526, 1723, 1841, 1920, 2103, 2376
Bse21I	CCTNAGG	2 cut(s)	654, 1287
Bse3DI	GCAATG	1 cut(s)	918
Bse8I	GATNNNNATC	2 cut(s)	237, 1959
BseBI	CCWGG	4 cut(s)	597, 1491, 1503, 1668
BseCI	ATCGAT	2 cut(s)	1200, 1661
BseDI	CCNNGG	5 cut(s)	102, 447, 1264, 1325, 2058
BseJI	GATNNNNATC	2 cut(s)	237, 1959
BseLI	CCNNNNNNNGG	7 cut(s)	498, 499, 518, 548, 1070, 1673, 1718
BseMI	GCAATG	1 cut(s)	918
BseMII	CTCAG	3 cut(s)	645, 1958, 2415
BseNI	ACTGG	9 cut(s)	523, 1439, 1449, 1526, 1723, 1841, 1920, 2103, 2376
BseRI	GAGGAG	1 cut(s)	1792
BseXI	GCAGC	3 cut(s)	773, 813, 1259
BseYI	CCCAGC	1 cut(s)	380
BsgI	GTGCAG	1 cut(s)	889
BshFI	GGCC	2 cut(s)	101, 1321
BshTI	ACCGGT	1 cut(s)	263
BshVI	ATCGAT	2 cut(s)	1200, 1661
BsiHKAI	GWGCWC	2 cut(s)	79, 2430
BsiHKCI	CYCGRG	2 cut(s)	446, 2347
BsiSI	CCGG	1 cut(s)	264
BslFI	GGGAC	1 cut(s)	831
BslI	CCNNNNNNNGG	7 cut(s)	498, 499, 518, 548, 1070, 1673, 1718
BsmAI	GTCTC	5 cut(s)	395, 936, 1018, 1942, 1947
BsmFI	GGGAC	1 cut(s)	831
BsnI	GGCC	2 cut(s)	101, 1321
Bso31I	GGTCTC	1 cut(s)	1947
BsoBI	CYCGRG	2 cut(s)	446, 2347
Bsp1286I	GDGCHC	3 cut(s)	79, 1534, 2430
Bsp1720I	GCTNAGC	1 cut(s)	1093
Bsp19I	CCATGG	1 cut(s)	102
BspACI	CCGC	6 cut(s)	107, 205, 303, 389, 493, 1550
BspANI	GGCC	2 cut(s)	101, 1321
BspCNI	CTCAG	3 cut(s)	646, 1957, 2416
BspDI	ATCGAT	2 cut(s)	1200, 1661
BspHI	TCATGA	1 cut(s)	2461
BspLI	GGNNCC	3 cut(s)	540, 1233, 1411
BspMAI	CTGCAG	2 cut(s)	337, 1342
BspMI	ACCTGC	1 cut(s)	1331
BspPI	GGATC	6 cut(s)	346, 689, 1395, 1597, 1764, 2359
BspTNI	GGTCTC	1 cut(s)	1947
BsrDI	GCAATG	1 cut(s)	918
BsrFI	RCCGGY	1 cut(s)	263
BsrI	ACTGG	9 cut(s)	523, 1439, 1449, 1526, 1723, 1841, 1920, 2103, 2376
BssAI	RCCGGY	1 cut(s)	263
BssECI	CCNNGG	5 cut(s)	102, 447, 1264, 1325, 2058
BssNAI	GTATAC	1 cut(s)	815
BssNI	GRCGYC	1 cut(s)	255
BssT1I	CCWWGG	3 cut(s)	102, 1264, 1325
Bst1107I	GTATAC	1 cut(s)	815
Bst2UI	CCWGG	4 cut(s)	597, 1491, 1503, 1668
Bst6I	CTCTTC	5 cut(s)	1713, 2003, 2112, 2161, 2227
BstACI	GRCGYC	1 cut(s)	255
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstC8I	GCNNGC	5 cut(s)	493, 543, 791, 909, 1277
BstDEI	CTNAG	6 cut(s)	654, 902, 1093, 1287, 1944, 2424
BstDSI	CCRYGG	2 cut(s)	102, 2058
BstENI	CCTNNNNNAGG	1 cut(s)	1068
BstHHI	GCGC	2 cut(s)	1135, 2457
BstMAI	GTCTC	5 cut(s)	395, 936, 1018, 1942, 1947
BstMWI	GCNNNNNNNGC	3 cut(s)	341, 747, 1103
BstNI	CCWGG	4 cut(s)	597, 1491, 1503, 1668
BstNSI	RCATGY	4 cut(s)	350, 716, 1162, 1639
BstSCI	CCNGG	4 cut(s)	595, 1489, 1501, 1666
BstSFI	CTRYAG	3 cut(s)	333, 1338, 2390
BstV1I	GCAGC	3 cut(s)	773, 813, 1259
BstV2I	GAAGAC	4 cut(s)	238, 672, 1731, 1839
BstX2I	RGATCY	3 cut(s)	880, 1756, 2351
BstXI	CCANNNNNNTGG	1 cut(s)	1521
BstYI	RGATCY	3 cut(s)	880, 1756, 2351
BstZ17I	GTATAC	1 cut(s)	815
Bsu15I	ATCGAT	2 cut(s)	1200, 1661
Bsu36I	CCTNAGG	2 cut(s)	654, 1287
BsuI	GTATCC	2 cut(s)	447, 1205
BsuRI	GGCC	2 cut(s)	101, 1321
BsuTUI	ATCGAT	2 cut(s)	1200, 1661
BtgI	CCRYGG	2 cut(s)	102, 2058
BtrI	CACGTC	1 cut(s)	433
BtsI	GCAGTG	2 cut(s)	2180, 2246
BtsIMutI	CAGTG	7 cut(s)	945, 1927, 2119, 2180, 2246, 2374, 2383
BveI	ACCTGC	1 cut(s)	1331
Cac8I	GCNNGC	5 cut(s)	493, 543, 791, 909, 1277
CaiI	CAGNNNCTG	1 cut(s)	1103
CciI	TCATGA	1 cut(s)	2461
CfoI	GCGC	2 cut(s)	1135, 2457
Cfr10I	RCCGGY	1 cut(s)	263
Cfr13I	GGNCC	1 cut(s)	916
ClaI	ATCGAT	2 cut(s)	1200, 1661
CseI	GACGC	1 cut(s)	263
Csp6I	GTAC	3 cut(s)	4, 274, 2518
CspAI	ACCGGT	1 cut(s)	263
CviAII	CATG	9 cut(s)	103, 347, 611, 713, 1159, 1577, 1636, 1651, 2462
CviQI	GTAC	3 cut(s)	4, 274, 2518
DdeI	CTNAG	6 cut(s)	654, 902, 1093, 1287, 1944, 2424
EaeI	YGGCCR	1 cut(s)	99
Eam1104I	CTCTTC	5 cut(s)	1713, 2003, 2112, 2161, 2227
EarI	CTCTTC	5 cut(s)	1713, 2003, 2112, 2161, 2227
EciI	GGCGGA	2 cut(s)	194, 378
Ecl136II	GAGCTC	1 cut(s)	77
Eco130I	CCWWGG	3 cut(s)	102, 1264, 1325
Eco24I	GRGCYC	2 cut(s)	79, 1534
Eco31I	GGTCTC	1 cut(s)	1947
Eco32I	GATATC	1 cut(s)	2219
Eco47I	GGWCC	1 cut(s)	916
Eco53kI	GAGCTC	1 cut(s)	77
Eco81I	CCTNAGG	2 cut(s)	654, 1287
Eco88I	CYCGRG	2 cut(s)	446, 2347
EcoICRI	GAGCTC	1 cut(s)	77
EcoNI	CCTNNNNNAGG	1 cut(s)	1068
EcoRI	GAATTC	2 cut(s)	996, 2307
EcoRII	CCWGG	4 cut(s)	595, 1489, 1501, 1666
EcoRV	GATATC	1 cut(s)	2219
EcoT14I	CCWWGG	3 cut(s)	102, 1264, 1325
EcoT38I	GRGCYC	2 cut(s)	79, 1534
ErhI	CCWWGG	3 cut(s)	102, 1264, 1325
FaeI	CATG	9 cut(s)	106, 350, 614, 716, 1162, 1580, 1639, 1654, 2465
FaqI	GGGAC	1 cut(s)	831
FatI	CATG	9 cut(s)	102, 346, 610, 712, 1158, 1576, 1635, 1650, 2461
FauI	CCCGC	2 cut(s)	500, 1557
FauNDI	CATATG	1 cut(s)	1237
FbaI	TGATCA	1 cut(s)	66
FblI	GTMKAC	3 cut(s)	150, 408, 814
Fnu4HI	GCNGC	4 cut(s)	304, 787, 827, 1248
FriOI	GRGCYC	2 cut(s)	79, 1534
Fsp4HI	GCNGC	4 cut(s)	304, 787, 827, 1248
FspBI	CTAG	2 cut(s)	2256, 2442
GlaI	GCGC	2 cut(s)	1134, 2456
GluI	GCNGC	4 cut(s)	304, 787, 827, 1248
GsaI	CCCAGC	1 cut(s)	384
GsuI	CTGGAG	5 cut(s)	963, 1903, 2086, 2131, 2197
HaeIII	GGCC	2 cut(s)	101, 1321
HapII	CCGG	1 cut(s)	264
HgaI	GACGC	1 cut(s)	263
HhaI	GCGC	2 cut(s)	1135, 2457
Hin1I	GRCGYC	1 cut(s)	255
Hin1II	CATG	9 cut(s)	106, 350, 614, 716, 1162, 1580, 1639, 1654, 2465
Hin6I	GCGC	2 cut(s)	1133, 2455
HinP1I	GCGC	2 cut(s)	1133, 2455
HincII	GTYRAC	3 cut(s)	151, 409, 430
HindII	GTYRAC	3 cut(s)	151, 409, 430
HpaII	CCGG	1 cut(s)	264
HphI	GGTGA	3 cut(s)	1107, 1733, 2195
Hpy166II	GTNNAC	8 cut(s)	151, 276, 409, 430, 815, 1454, 1894, 2525
Hpy8I	GTNNAC	8 cut(s)	151, 276, 409, 430, 815, 1454, 1894, 2525
Hpy99I	CGWCG	2 cut(s)	152, 257
HpyAV	CCTTC	4 cut(s)	152, 466, 829, 1757
HpyCH4IV	ACGT	2 cut(s)	432, 1598
HpyF10VI	GCNNNNNNNGC	3 cut(s)	341, 747, 1103
HpyF3I	CTNAG	6 cut(s)	654, 902, 1093, 1287, 1944, 2424
HpySE526I	ACGT	2 cut(s)	432, 1598
Hsp92I	GRCGYC	1 cut(s)	255
Hsp92II	CATG	9 cut(s)	106, 350, 614, 716, 1162, 1580, 1639, 1654, 2465
HspAI	GCGC	2 cut(s)	1133, 2455
Ksp22I	TGATCA	1 cut(s)	66
LmnI	GCTCC	9 cut(s)	538, 982, 1237, 1415, 1493, 2105, 2150, 2216, 2400
Lsp1109I	GCAGC	3 cut(s)	773, 813, 1259
LweI	GCATC	7 cut(s)	466, 478, 531, 577, 1120, 1855, 1969
MaeI	CTAG	2 cut(s)	2256, 2442
MaeII	ACGT	2 cut(s)	432, 1598
MaeIII	GTNAC	5 cut(s)	1109, 1922, 2177, 2342, 2405
MflI	RGATCY	3 cut(s)	880, 1756, 2351
MhlI	GDGCHC	3 cut(s)	79, 1534, 2430
MlyI	GAGTC	4 cut(s)	89, 938, 1052, 2272
MmeI	TCCRAC	2 cut(s)	276, 2240
MroXI	GAANNNNTTC	2 cut(s)	604, 1786
MseI	TTAA	3 cut(s)	672, 941, 1424
MslI	CAYNNNNRTG	3 cut(s)	609, 1146, 2037
MspI	CCGG	1 cut(s)	264
MspR9I	CCNGG	4 cut(s)	597, 1491, 1503, 1668
MvaI	CCWGG	4 cut(s)	597, 1491, 1503, 1668
MwoI	GCNNNNNNNGC	3 cut(s)	341, 747, 1103
NcoI	CCATGG	1 cut(s)	102
NdeI	CATATG	1 cut(s)	1237
NlaIII	CATG	9 cut(s)	106, 350, 614, 716, 1162, 1580, 1639, 1654, 2465
NlaIV	GGNNCC	3 cut(s)	540, 1233, 1411
NmeAIII	GCCGAG	2 cut(s)	77, 1223
NmuCI	GTSAC	4 cut(s)	1109, 1922, 2177, 2405
NspI	RCATGY	4 cut(s)	350, 716, 1162, 1639
PagI	TCATGA	1 cut(s)	2461
PaqCI	CACCTGC	1 cut(s)	1331
PciI	ACATGT	1 cut(s)	346
PcsI	WCGNNNNNNNCGW	3 cut(s)	147, 249, 438
PdmI	GAANNNNTTC	2 cut(s)	604, 1786
PfeI	GAWTC	9 cut(s)	90, 143, 233, 385, 989, 1079, 1100, 1663, 2252
PflMI	CCANNNNNTGG	2 cut(s)	518, 1673
PfoI	TCCNGGA	2 cut(s)	595, 1489
PinAI	ACCGGT	1 cut(s)	263
PkrI	GCNGC	4 cut(s)	305, 788, 828, 1249
PleI	GAGTC	4 cut(s)	89, 937, 1051, 2271
PpsI	GAGTC	4 cut(s)	89, 937, 1051, 2271
PscI	ACATGT	1 cut(s)	346
Psp124BI	GAGCTC	1 cut(s)	79
Psp6I	CCWGG	4 cut(s)	595, 1489, 1501, 1666
PspFI	CCCAGC	1 cut(s)	380
PspGI	CCWGG	4 cut(s)	595, 1489, 1501, 1666
PspN4I	GGNNCC	3 cut(s)	540, 1233, 1411
PspPI	GGNCC	1 cut(s)	916
PsrI	GAACNNNNNNTAC	2 cut(s)	615, 647
PstI	CTGCAG	2 cut(s)	337, 1342
PstNI	CAGNNNCTG	1 cut(s)	1103
PsuI	RGATCY	3 cut(s)	880, 1756, 2351
RsaI	GTAC	3 cut(s)	5, 275, 2519
RsaNI	GTAC	3 cut(s)	4, 274, 2518
RseI	CAYNNNNRTG	3 cut(s)	609, 1146, 2037
SacI	GAGCTC	1 cut(s)	79
SalI	GTCGAC	2 cut(s)	149, 407
SaqAI	TTAA	3 cut(s)	672, 941, 1424
SatI	GCNGC	4 cut(s)	304, 787, 827, 1248
Sau96I	GGNCC	1 cut(s)	916
SbfI	CCTGCAGG	1 cut(s)	337
SchI	GAGTC	4 cut(s)	89, 938, 1052, 2272
ScrFI	CCNGG	4 cut(s)	597, 1491, 1503, 1668
SdaI	CCTGCAGG	1 cut(s)	337
SduI	GDGCHC	3 cut(s)	79, 1534, 2430
SfaNI	GCATC	7 cut(s)	466, 478, 531, 577, 1120, 1855, 1969
SfcI	CTRYAG	3 cut(s)	333, 1338, 2390
SinI	GGWCC	1 cut(s)	916
SmiMI	CAYNNNNRTG	3 cut(s)	609, 1146, 2037
SmlI	CTYRAG	1 cut(s)	830
SmoI	CTYRAG	1 cut(s)	830
Sse8387I	CCTGCAGG	1 cut(s)	337
SsiI	CCGC	6 cut(s)	107, 205, 303, 389, 493, 1550
SspI	AATATT	1 cut(s)	289
SspMI	CTAG	2 cut(s)	2256, 2442
SstI	GAGCTC	1 cut(s)	79
StyD4I	CCNGG	4 cut(s)	595, 1489, 1501, 1666
StyI	CCWWGG	3 cut(s)	102, 1264, 1325
TaiI	ACGT	2 cut(s)	435, 1601
TaqI	TCGA	8 cut(s)	93, 141, 150, 156, 163, 408, 1200, 1661
TatI	WGTACW	1 cut(s)	273
TauI	GCSGC	1 cut(s)	306
TfiI	GAWTC	9 cut(s)	90, 143, 233, 385, 989, 1079, 1100, 1663, 2252
Tru1I	TTAA	3 cut(s)	672, 941, 1424
Tru9I	TTAA	3 cut(s)	672, 941, 1424
TscAI	CASTG	7 cut(s)	952, 1927, 2119, 2180, 2246, 2374, 2383
TseFI	GTSAC	4 cut(s)	1109, 1922, 2177, 2405
TseI	GCWGC	3 cut(s)	786, 826, 1247
Tsp45I	GTSAC	4 cut(s)	1109, 1922, 2177, 2405
TspDTI	ATGAA	9 cut(s)	522, 749, 1457, 1616, 1845, 1979, 2175, 2478, 2508
TspGWI	ACGGA	7 cut(s)	23, 136, 288, 301, 1584, 2075, 2542
TspRI	CASTG	7 cut(s)	952, 1927, 2119, 2180, 2246, 2374, 2383
Van91I	CCANNNNNTGG	2 cut(s)	518, 1673
VpaK11BI	GGWCC	1 cut(s)	916
XagI	CCTNNNNNAGG	1 cut(s)	1068
XceI	RCATGY	4 cut(s)	350, 716, 1162, 1639
XmiI	GTMKAC	3 cut(s)	150, 408, 814
XmnI	GAANNNNTTC	2 cut(s)	604, 1786
XspI	CTAG	2 cut(s)	2256, 2442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.