Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009656

Physical Location & Seq

Genomic Coordinates Forward (+)

3552717 .. 3557404

Gene Structure 4688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1051500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGGCGGTCCAGTTCAAATTCAGAAGCTCCGTCAACTTTGAATCCGTCGCCATCGACGGCCGCGCCTCCATCTCCGTTCGCGATCTCAAATCCAAGATAATTCGCCACAAGAACCTCAACCTCTGCCAAGACTCCGAACTCGTCTTCTCTGACGCCGTAACCGGTCAAGAGTACAACGACGAGAGCATTCAAATTCCTTGCGGTTCTAGCGTGGTAATTAAGCGAGTTCCTGCAGGATCAGTACATGTGAATCAGCCTCACTTCAATTCATGTCAGAATCTTCATAGCAAGGACTCTGTGGATGCTAAATCACCCGCTGCACCGAATGCTAAGACTGTTGACTTTGATGACTTTGGGGTTGACTTGTATCCCATTCCTAAAGAAATCGTTTTCAGTTCTGATTTGGATACGGACAAGGATAACTTCATCTATGATTATCAACCAAATACCGATAACGGCATCAAAAGATATTCGGAGCCCGCTATGAGACGATGCTTGAAACTTGAAGCAAGTAGCATTAGTGATGCTTTTCCAGGAGTTCATGTGCATAGTGGAGTTGAACAAATCATATTGCCGACAAAATCAAAGCCTGAAGAAGAAGACATGAATGTGGAAAGGGTGGGCAGTGCGACTGCCCTAGATACACAGCATGCTAATTTGTCATCAGAGCTGAAATGCTCTCTATGCAACGCATTTTTCAAGGAGGCCGTAATGATACCATGCTGCCAGCACAGTTTTTGTGAGAAATGCATTTCTCAAGTGTTGCGAGAGAAGACCAGATGTCCCAAGTGTCTTTTGACCAAATGCAGAGTAGAAGATTTGCTGCCAAATGTTTCTCTGAGGCAAGCAATTGAGCATTTCCTAGAATCTCAAAATTTAATCACTGCTCCAGATAATGATTGTTGCCAGTATGCTCCAGATGGAGAATCTGGAATTCAGGCCAAAGATGTGTCTCGGGGCGGTAATATTTTACAAAGAGAGGCAGAGGCGCCTCATTCTCCTGAAACAGGGACAGGTTCTAATCATTTATTTGCAGAATCTGCTTTTAACCCTCCTTTCCAAAAAGATGTTAAACCAGCAATCACAAAAAGGAGGCCACCTTGGGTTTCTTCAGAAGGTGGTGACAAGAGCTTCTCAGAGACTGGAAAGCACAGAAAGGGGGAACGTACCTGTTACAGGTGTGGTTCGCCAGACCATTTCATCAGAGACTGTCCAGTTGCTTCAAGCCCACATCCTATGCTTCATGCAGGAAATGCAATCTTTCCAGGAGCTGTGCCAGGCTATGTACCACCATACTGGAATGGGGCTCCCTCACCTCATGCCATGCCCTTTGGAAATCCATATGGAAATCATGCGAGGATGGCTTTTGGTACAAATATGGTCCCTCCTGGTCCTTATGCTGTTCCTGCATACATGCCATCTATGTATAACTCATTCCCTTCCTTTGGTGGATATATGAGGATGGGAGGTGTTGCTCCTCTTCCAGTGACTGGTGAAGACTGGCATTTAAGTCGTACGGAATCCGTGGATCTTCATGGTCGGGGAAAGAGAAGAAAAGTTTCAAATGAGAATTTGAGGAGAGAACAATCTCATGATGGTGAAGATGACGAAGACCGTGATTTAAACGAGAGACATTACTATAATGGGACGGAAAAAATACATGACCACAGATCTCGTATAAACAGGGAAATGAGCATAAGTTACTCTGAGGATGGCTCTACTAAAGGGTCCCAGAGGCAATTTCGGCATCACAATCACACAGATGAAGACAGGCTTGCAGTTCAAGGTCAGCAGAAGAGTTCCCGTTTGGTGGTTGATGGTCAAGATCAGAAGCAGTATCATCGCACGGAAGGATCAAGTTCAGAATTAGACGATGTGCCAAGTAGCTCCGGTTGCCACAGTGAAGAGAGACAGAAGCACCACCATAGAAGCTCCAGAAAGCATAAGGATCAAAGAGAGCAGTGTGGCAGTGATTCCAGTCAGAGTCATCATCGATATACCAAAGACAATAACAGAAACAGGATCGAGCATGAATCCAAAAGGCACAATCAGAAATATCGCAGCCACTCAGGATATGGTATTGATCAAAGTGGTTCCAGTGATAAGAAGTTGCAGAAAGAGAGCAGTCACAGTTCTAGACATTCTAAGCTCAGTGGAAAGAGTAATGTCGATGAGCGGAGTCATGACAGGTGGAAGATGGTTAGTGGGTCAGATGAAGATTGTGGAGAAGGTTATCCGTATTCTAGACAGAAAAGAAAACACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001701 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006275 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009792 GO:0009888 GO:0009889 GO:0009987 GO:0010556 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0035264 GO:0036211 GO:0040007 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048513 GO:0048568 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051603 GO:0051716 GO:0060255 GO:0060429 GO:0061053 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DWNN zf-C3HC4_2 zf-CCHC

Protein Analysis

755

Amino Acids

84.66

Weight (kDa)

7.12

Isoelectric Point (pI)

55.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DWNN	PF08783	3 - 77	3.6e-20	DWNN domain
zf-C3HC4_2	PF13923	225 - 264	2.3e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	226 - 264	3.2e-06	Zinc finger, C3HC4 type (RING finger)
zf-CCHC	PF00098	389 - 405	3.8e-06	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120 FvH4_2g31120
malus_domestica	MD08G1070600.v1.1 MD15G1051500.v1.1
prunus_persica	Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1 Prupe.1G405500_v2.0.a1
pyrus_communis	pycom08g05840 pycom15g04930
rosa_chinensis	RchiOBHm_Chr6g0311661
rosa_laevigata	RLG00000010383
rosa_roxburghii	Rroxscaffold_7G00157400
rosa_rugosa	Rorug06G0390500.1
rosa_samantha	Rh6AG505800 Rh6BG516600 Rh6CG522500 Rh6DG508200
rosa_wichuraiana	Rw6G044020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	988
AccB7I	CCANNNNNTGG	1 cut(s)	1490
AccBSI	CCGCTC	1 cut(s)	2176
AccII	CGCG	2 cut(s)	63, 81
AciI	CCGC	7 cut(s)	5, 61, 201, 315, 480, 960, 2176
AclWI	GGATC	5 cut(s)	244, 1536, 1861, 1956, 2030
AcoI	YGGCCR	1 cut(s)	58
AcsI	RAATTY	5 cut(s)	17, 192, 874, 933, 1570
AcuI	CTGAAG	2 cut(s)	612, 1095
AcyI	GRCGYC	2 cut(s)	153, 989
AfaI	GTAC	6 cut(s)	173, 243, 1168, 1287, 1372, 1516
AfiI	CCNNNNNNNGG	5 cut(s)	1007, 1176, 1445, 1490, 1810
AflIII	ACRYGT	1 cut(s)	244
AgeI	ACCGGT	1 cut(s)	161
AhdI	GACNNNNNGTC	1 cut(s)	780
AjnI	CCWGG	4 cut(s)	532, 1264, 1276, 1387
AluBI	AGCT	7 cut(s)	27, 670, 1131, 1271, 1887, 1932, 2149
AluI	AGCT	7 cut(s)	27, 670, 1131, 1271, 1887, 1932, 2149
Alw26I	GTCTC	6 cut(s)	481, 957, 1133, 1200, 1624, 1903
AlwI	GGATC	5 cut(s)	244, 1536, 1861, 1956, 2030
AlwNI	CAGNNNCTG	5 cut(s)	1040, 1142, 1209, 1271, 1490
Ama87I	CYCGRG	1 cut(s)	954
AoxI	GGCC	4 cut(s)	58, 705, 939, 1094
ApeKI	GCWGC	4 cut(s)	317, 723, 823, 2061
ApoI	RAATTY	5 cut(s)	17, 192, 874, 933, 1570
AsiGI	ACCGGT	1 cut(s)	161
Asp700I	GAANNNNTTC	1 cut(s)	1558
AspLEI	GCGC	2 cut(s)	65, 991
AspS9I	GGNCC	4 cut(s)	7, 1381, 1391, 1728
AsuHPI	GGTGA	5 cut(s)	303, 1133, 1305, 1505, 1610
AvaI	CYCGRG	1 cut(s)	954
AvaII	GGWCC	4 cut(s)	7, 1381, 1391, 1728
BanI	GGYRCC	1 cut(s)	988
BanII	GRGCYC	2 cut(s)	480, 1309
BbsI	GAAGAC	6 cut(s)	136, 606, 779, 1503, 1617, 1773
BbvI	GCAGC	4 cut(s)	304, 710, 810, 2073
BceAI	ACGGC	4 cut(s)	73, 140, 472, 692
BcgI	CGANNNNNNTGC	4 cut(s)	1493, 1527, 1823, 1857
BciT130I	CCWGG	4 cut(s)	534, 1266, 1278, 1389
BciVI	GTATCC	2 cut(s)	378, 400
BclI	TGATCA	1 cut(s)	2083
BcoDI	GTCTC	6 cut(s)	481, 957, 1133, 1200, 1624, 1903
BfaI	CTAG	6 cut(s)	207, 638, 863, 2136, 2244, 2263
BfmI	CTRYAG	1 cut(s)	231
BfoI	RGCGCY	1 cut(s)	992
BfuI	GTATCC	2 cut(s)	378, 400
BglII	AGATCT	1 cut(s)	1670
BisI	GCNGC	5 cut(s)	61, 318, 724, 824, 2062
BlsI	GCNGC	5 cut(s)	62, 319, 725, 825, 2063
Bme1390I	CCNGG	4 cut(s)	534, 1266, 1278, 1389
Bme18I	GGWCC	4 cut(s)	7, 1381, 1391, 1728
BmeRI	GACNNNNNGTC	1 cut(s)	780
BmeT110I	CYCGRG	1 cut(s)	954
BmgT120I	GGNCC	4 cut(s)	7, 1381, 1391, 1728
BmiI	GGNNCC	7 cut(s)	477, 990, 1308, 1383, 1729, 1730, 2095
BmrFI	CCNGG	4 cut(s)	534, 1266, 1278, 1389
BmsI	GCATC	5 cut(s)	292, 468, 482, 514, 1756
BpiI	GAAGAC	6 cut(s)	136, 606, 779, 1503, 1617, 1773
BplI	GAGNNNNNCTC	2 cut(s)	1700, 1732
BpmI	CTGGAG	3 cut(s)	873, 900, 1918
BpuEI	CTTGAG	1 cut(s)	741
Bsa29I	ATCGAT	1 cut(s)	1993
BsaHI	GRCGYC	2 cut(s)	153, 989
BsaJI	CCNNGG	2 cut(s)	1100, 1524
BsaWI	WCCGGW	2 cut(s)	161, 1889
BsaXI	ACNNNNNCTCC	2 cut(s)	528, 558
Bsc4I	CCNNNNNNNGG	5 cut(s)	1007, 1176, 1445, 1490, 1810
Bse118I	RCCGGY	1 cut(s)	161
BseBI	CCWGG	4 cut(s)	534, 1266, 1278, 1389
BseCI	ATCGAT	1 cut(s)	1993
BseDI	CCNNGG	2 cut(s)	1100, 1524
BseGI	GGATG	5 cut(s)	307, 1231, 1365, 1467, 1717
BseLI	CCNNNNNNNGG	5 cut(s)	1007, 1176, 1445, 1490, 1810
BseMII	CTCAG	5 cut(s)	830, 1149, 1698, 2082, 2164
BseRI	GAGGAG	2 cut(s)	1467, 1591
BseX3I	CGGCCG	1 cut(s)	58
BseXI	GCAGC	4 cut(s)	304, 710, 810, 2073
BsgI	GTGCAG	1 cut(s)	303
Bsh1236I	CGCG	2 cut(s)	63, 81
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	4 cut(s)	60, 707, 941, 1096
BshNI	GGYRCC	1 cut(s)	988
BshTI	ACCGGT	1 cut(s)	161
BshVI	ATCGAT	1 cut(s)	1993
BsiEI	CGRYCG	1 cut(s)	61
BsiHKCI	CYCGRG	1 cut(s)	954
BsiSI	CCGG	2 cut(s)	162, 1890
BsiWI	CGTACG	1 cut(s)	1514
BslFI	GGGAC	5 cut(s)	768, 1024, 1367, 1660, 1714
BslI	CCNNNNNNNGG	5 cut(s)	1007, 1176, 1445, 1490, 1810
BsmAI	GTCTC	6 cut(s)	481, 957, 1133, 1200, 1624, 1903
BsmBI	CGTCTC	1 cut(s)	481
BsmFI	GGGAC	5 cut(s)	768, 1024, 1367, 1660, 1714
BsmI	GAATGC	2 cut(s)	186, 331
BsnI	GGCC	4 cut(s)	60, 707, 941, 1096
BsoBI	CYCGRG	1 cut(s)	954
Bsp1286I	GDGCHC	2 cut(s)	480, 1309
Bsp143I	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
Bsp68I	TCGCGA	1 cut(s)	81
BspACI	CCGC	7 cut(s)	5, 61, 201, 315, 480, 960, 2176
BspANI	GGCC	4 cut(s)	60, 707, 941, 1096
BspCNI	CTCAG	5 cut(s)	831, 1148, 1699, 2081, 2163
BspDI	ATCGAT	1 cut(s)	1993
BspFNI	CGCG	2 cut(s)	63, 81
BspHI	TCATGA	2 cut(s)	1591, 2182
BspLI	GGNNCC	7 cut(s)	477, 990, 1308, 1383, 1729, 1730, 2095
BspMAI	CTGCAG	1 cut(s)	235
BspPI	GGATC	5 cut(s)	244, 1536, 1861, 1956, 2030
BspT107I	GGYRCC	1 cut(s)	988
BsrBI	CCGCTC	1 cut(s)	2176
BsrFI	RCCGGY	1 cut(s)	161
BssAI	RCCGGY	1 cut(s)	161
BssECI	CCNNGG	2 cut(s)	1100, 1524
BssMI	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
BssNI	GRCGYC	2 cut(s)	153, 989
BssT1I	CCWWGG	1 cut(s)	1100
Bst2UI	CCWGG	4 cut(s)	534, 1266, 1278, 1389
Bst4CI	ACNGT	6 cut(s)	337, 734, 1211, 1616, 1901, 2132
Bst6I	CTCTTC	3 cut(s)	1485, 1790, 1899
BstACI	GRCGYC	2 cut(s)	153, 989
BstAPI	GCANNNNNTGC	3 cut(s)	326, 1040, 1253
BstC8I	GCNNGC	5 cut(s)	480, 651, 728, 846, 1776
BstDEI	CTNAG	7 cut(s)	330, 839, 1135, 1707, 2068, 2145, 2150
BstDSI	CCRYGG	1 cut(s)	1524
BstENI	CCTNNNNNAGG	2 cut(s)	1005, 1174
BstF5I	GGATG	5 cut(s)	307, 1231, 1365, 1467, 1717
BstFNI	CGCG	2 cut(s)	63, 81
BstH2I	RGCGCY	1 cut(s)	992
BstHHI	GCGC	2 cut(s)	65, 991
BstKTI	GATC	9 cut(s)	85, 239, 1531, 1673, 1828, 1856, 1951, 2025, 2086
BstMAI	GTCTC	6 cut(s)	481, 957, 1133, 1200, 1624, 1903
BstMBI	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
BstMCI	CGRYCG	1 cut(s)	61
BstMWI	GCNNNNNNNGC	9 cut(s)	207, 326, 684, 1040, 1253, 1361, 1406, 1744, 1893
BstNI	CCWGG	4 cut(s)	534, 1266, 1278, 1389
BstNSI	RCATGY	3 cut(s)	248, 653, 1417
BstSCI	CCNGG	4 cut(s)	532, 1264, 1276, 1387
BstSFI	CTRYAG	1 cut(s)	231
BstUI	CGCG	2 cut(s)	63, 81
BstV1I	GCAGC	4 cut(s)	304, 710, 810, 2073
BstV2I	GAAGAC	6 cut(s)	136, 606, 779, 1503, 1617, 1773
BstX2I	RGATCY	2 cut(s)	1528, 1670
BstXI	CCANNNNNNTGG	1 cut(s)	1296
BstYI	RGATCY	2 cut(s)	1528, 1670
BstZI	CGGCCG	1 cut(s)	58
Bsu15I	ATCGAT	1 cut(s)	1993
BsuI	GTATCC	2 cut(s)	378, 400
BsuRI	GGCC	4 cut(s)	60, 707, 941, 1096
BsuTUI	ATCGAT	1 cut(s)	1993
BtgI	CCRYGG	1 cut(s)	1524
BtgZI	GCGATG	1 cut(s)	1826
BtsCI	GGATG	5 cut(s)	307, 1231, 1365, 1467, 1717
BtsI	GCAGTG	4 cut(s)	631, 882, 1967, 1975
BtsIMutI	CAGTG	8 cut(s)	631, 882, 1491, 1906, 1967, 1975, 2104, 2158
BtuMI	TCGCGA	1 cut(s)	81
Cac8I	GCNNGC	5 cut(s)	480, 651, 728, 846, 1776
CaiI	CAGNNNCTG	5 cut(s)	1040, 1142, 1209, 1271, 1490
CciI	TCATGA	2 cut(s)	1591, 2182
CfoI	GCGC	2 cut(s)	65, 991
Cfr10I	RCCGGY	1 cut(s)	161
Cfr13I	GGNCC	4 cut(s)	7, 1381, 1391, 1728
ClaI	ATCGAT	1 cut(s)	1993
CseI	GACGC	1 cut(s)	161
Csp6I	GTAC	6 cut(s)	172, 242, 1167, 1286, 1371, 1515
CspAI	ACCGGT	1 cut(s)	161
CviQI	GTAC	6 cut(s)	172, 242, 1167, 1286, 1371, 1515
DdeI	CTNAG	7 cut(s)	330, 839, 1135, 1707, 2068, 2145, 2150
DinI	GGCGCC	1 cut(s)	990
DpnI	GATC	9 cut(s)	84, 238, 1530, 1672, 1827, 1855, 1950, 2024, 2085
DpnII	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
DraI	TTTAAA	1 cut(s)	1623
DriI	GACNNNNNGTC	1 cut(s)	780
EaeI	YGGCCR	1 cut(s)	58
EagI	CGGCCG	1 cut(s)	58
Eam1104I	CTCTTC	3 cut(s)	1485, 1790, 1899
Eam1105I	GACNNNNNGTC	1 cut(s)	780
EarI	CTCTTC	3 cut(s)	1485, 1790, 1899
EclXI	CGGCCG	1 cut(s)	58
Eco130I	CCWWGG	1 cut(s)	1100
Eco24I	GRGCYC	2 cut(s)	480, 1309
Eco47I	GGWCC	4 cut(s)	7, 1381, 1391, 1728
Eco52I	CGGCCG	1 cut(s)	58
Eco57I	CTGAAG	2 cut(s)	612, 1095
Eco88I	CYCGRG	1 cut(s)	954
EcoNI	CCTNNNNNAGG	2 cut(s)	1005, 1174
EcoO109I	RGGNCCY	1 cut(s)	1728
EcoRI	GAATTC	1 cut(s)	933
EcoRII	CCWGG	4 cut(s)	532, 1264, 1276, 1387
EcoT14I	CCWWGG	1 cut(s)	1100
EcoT22I	ATGCAT	1 cut(s)	752
EcoT38I	GRGCYC	2 cut(s)	480, 1309
EgeI	GGCGCC	1 cut(s)	990
EheI	GGCGCC	1 cut(s)	990
ErhI	CCWWGG	1 cut(s)	1100
Esp3I	CGTCTC	1 cut(s)	481
FalI	AAGNNNNNCTT	4 cut(s)	407, 439, 1758, 1790
FaqI	GGGAC	5 cut(s)	768, 1024, 1367, 1660, 1714
FauI	CCCGC	2 cut(s)	322, 487
FauNDI	CATATG	1 cut(s)	1342
FbaI	TGATCA	1 cut(s)	2083
Fnu4HI	GCNGC	5 cut(s)	61, 318, 724, 824, 2062
FokI	GGATG	5 cut(s)	314, 1218, 1372, 1474, 1724
FriOI	GRGCYC	2 cut(s)	480, 1309
Fsp4HI	GCNGC	5 cut(s)	61, 318, 724, 824, 2062
FspBI	CTAG	6 cut(s)	207, 638, 863, 2136, 2244, 2263
GlaI	GCGC	2 cut(s)	64, 990
GluI	GCNGC	5 cut(s)	61, 318, 724, 824, 2062
GsuI	CTGGAG	3 cut(s)	873, 900, 1918
HaeII	RGCGCY	1 cut(s)	992
HaeIII	GGCC	4 cut(s)	60, 707, 941, 1096
HapII	CCGG	2 cut(s)	162, 1890
HgaI	GACGC	1 cut(s)	161
HhaI	GCGC	2 cut(s)	65, 991
Hin1I	GRCGYC	2 cut(s)	153, 989
Hin6I	GCGC	2 cut(s)	63, 989
HinP1I	GCGC	2 cut(s)	63, 989
HincII	GTYRAC	3 cut(s)	34, 340, 361
HindII	GTYRAC	3 cut(s)	34, 340, 361
HpaII	CCGG	2 cut(s)	162, 1890
HphI	GGTGA	5 cut(s)	303, 1133, 1305, 1505, 1610
Hpy166II	GTNNAC	3 cut(s)	34, 340, 361
Hpy8I	GTNNAC	3 cut(s)	34, 340, 361
Hpy99I	CGWCG	3 cut(s)	50, 59, 182
HpyAV	CCTTC	4 cut(s)	1109, 1449, 1844, 2222
HpyCH4III	ACNGT	6 cut(s)	337, 734, 1211, 1616, 1901, 2132
HpyCH4IV	ACGT	1 cut(s)	1165
HpyF10VI	GCNNNNNNNGC	9 cut(s)	207, 326, 684, 1040, 1253, 1361, 1406, 1744, 1893
HpyF3I	CTNAG	7 cut(s)	330, 839, 1135, 1707, 2068, 2145, 2150
HpySE526I	ACGT	1 cut(s)	1165
Hsp92I	GRCGYC	2 cut(s)	153, 989
HspAI	GCGC	2 cut(s)	63, 989
KasI	GGCGCC	1 cut(s)	988
KflI	GGGWCCC	1 cut(s)	1728
Ksp22I	TGATCA	1 cut(s)	2083
Kzo9I	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
LmnI	GCTCC	9 cut(s)	32, 475, 892, 919, 1268, 1312, 1480, 1892, 1937
Lsp1109I	GCAGC	4 cut(s)	304, 710, 810, 2073
LweI	GCATC	5 cut(s)	292, 468, 482, 514, 1756
MaeI	CTAG	6 cut(s)	207, 638, 863, 2136, 2244, 2263
MaeII	ACGT	1 cut(s)	1165
MaeIII	GTNAC	6 cut(s)	157, 1121, 1172, 1486, 1700, 2126
MalI	GATC	9 cut(s)	84, 238, 1530, 1672, 1827, 1855, 1950, 2024, 2085
MbiI	CCGCTC	1 cut(s)	2176
MboI	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
MfeI	CAATTG	1 cut(s)	849
MflI	RGATCY	2 cut(s)	1528, 1670
MhlI	GDGCHC	2 cut(s)	480, 1309
Mly113I	GGCGCC	1 cut(s)	989
MlyI	GAGTC	4 cut(s)	125, 287, 1993, 2188
Mph1103I	ATGCAT	1 cut(s)	752
MroXI	GAANNNNTTC	1 cut(s)	1558
MseI	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
MslI	CAYNNNNRTG	3 cut(s)	608, 1596, 1761
MspA1I	CMGCKG	1 cut(s)	317
MspI	CCGG	2 cut(s)	162, 1890
MspR9I	CCNGG	4 cut(s)	534, 1266, 1278, 1389
MunI	CAATTG	1 cut(s)	849
Mva1269I	GAATGC	2 cut(s)	186, 331
MvaI	CCWGG	4 cut(s)	534, 1266, 1278, 1389
MvnI	CGCG	2 cut(s)	63, 81
MwoI	GCNNNNNNNGC	9 cut(s)	207, 326, 684, 1040, 1253, 1361, 1406, 1744, 1893
NarI	GGCGCC	1 cut(s)	989
NdeI	CATATG	1 cut(s)	1342
NdeII	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
NlaIV	GGNNCC	7 cut(s)	477, 990, 1308, 1383, 1729, 1730, 2095
NmuCI	GTSAC	3 cut(s)	1121, 1486, 2126
NruI	TCGCGA	1 cut(s)	81
NsiI	ATGCAT	1 cut(s)	752
NspI	RCATGY	3 cut(s)	248, 653, 1417
PaeI	GCATGC	1 cut(s)	653
PagI	TCATGA	2 cut(s)	1591, 2182
PciI	ACATGT	1 cut(s)	244
PctI	GAATGC	2 cut(s)	186, 331
PdmI	GAANNNNTTC	1 cut(s)	1558
PfeI	GAWTC	9 cut(s)	41, 250, 277, 866, 926, 1037, 1520, 1973, 2033
Pfl23II	CGTACG	1 cut(s)	1514
PflMI	CCANNNNNTGG	1 cut(s)	1490
PfoI	TCCNGGA	2 cut(s)	532, 1264
PinAI	ACCGGT	1 cut(s)	161
PkrI	GCNGC	5 cut(s)	62, 319, 725, 825, 2063
PleI	GAGTC	4 cut(s)	125, 287, 1992, 2187
PluTI	GGCGCC	1 cut(s)	992
PpsI	GAGTC	4 cut(s)	125, 287, 1992, 2187
PpuMI	RGGWCCY	1 cut(s)	1728
PscI	ACATGT	1 cut(s)	244
Psp5II	RGGWCCY	1 cut(s)	1728
Psp6I	CCWGG	4 cut(s)	532, 1264, 1276, 1387
PspGI	CCWGG	4 cut(s)	532, 1264, 1276, 1387
PspLI	CGTACG	1 cut(s)	1514
PspN4I	GGNNCC	7 cut(s)	477, 990, 1308, 1383, 1729, 1730, 2095
PspPI	GGNCC	4 cut(s)	7, 1381, 1391, 1728
PspPPI	RGGWCCY	1 cut(s)	1728
PstI	CTGCAG	1 cut(s)	235
PstNI	CAGNNNCTG	5 cut(s)	1040, 1142, 1209, 1271, 1490
PsuI	RGATCY	2 cut(s)	1528, 1670
RruI	TCGCGA	1 cut(s)	81
RsaI	GTAC	6 cut(s)	173, 243, 1168, 1287, 1372, 1516
RsaNI	GTAC	6 cut(s)	172, 242, 1167, 1286, 1371, 1515
RseI	CAYNNNNRTG	3 cut(s)	608, 1596, 1761
SaqAI	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
SatI	GCNGC	5 cut(s)	61, 318, 724, 824, 2062
Sau3AI	GATC	9 cut(s)	82, 236, 1528, 1670, 1825, 1853, 1948, 2022, 2083
Sau96I	GGNCC	4 cut(s)	7, 1381, 1391, 1728
SbfI	CCTGCAGG	1 cut(s)	235
SchI	GAGTC	4 cut(s)	125, 287, 1993, 2188
ScrFI	CCNGG	4 cut(s)	534, 1266, 1278, 1389
SdaI	CCTGCAGG	1 cut(s)	235
SduI	GDGCHC	2 cut(s)	480, 1309
SfaNI	GCATC	5 cut(s)	292, 468, 482, 514, 1756
SfcI	CTRYAG	1 cut(s)	231
SfoI	GGCGCC	1 cut(s)	990
SinI	GGWCC	4 cut(s)	7, 1381, 1391, 1728
SmiMI	CAYNNNNRTG	3 cut(s)	608, 1596, 1761
SmlI	CTYRAG	1 cut(s)	756
SmoI	CTYRAG	1 cut(s)	756
SphI	GCATGC	1 cut(s)	653
Sse8387I	CCTGCAGG	1 cut(s)	235
SsiI	CCGC	7 cut(s)	5, 61, 201, 315, 480, 960, 2176
SspDI	GGCGCC	1 cut(s)	988
SspI	AATATT	1 cut(s)	967
SspMI	CTAG	6 cut(s)	207, 638, 863, 2136, 2244, 2263
StyD4I	CCNGG	4 cut(s)	532, 1264, 1276, 1387
StyI	CCWWGG	1 cut(s)	1100
TaaI	ACNGT	6 cut(s)	337, 734, 1211, 1616, 1901, 2132
TaiI	ACGT	1 cut(s)	1168
TaqI	TCGA	4 cut(s)	54, 1993, 2025, 2169
TatI	WGTACW	2 cut(s)	171, 241
TauI	GCSGC	1 cut(s)	63
TfiI	GAWTC	9 cut(s)	41, 250, 277, 866, 926, 1037, 1520, 1973, 2033
Tru1I	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
Tru9I	TTAA	6 cut(s)	219, 878, 1047, 1071, 1508, 1622
TscAI	CASTG	8 cut(s)	631, 889, 1491, 1906, 1967, 1975, 2104, 2158
TseFI	GTSAC	3 cut(s)	1121, 1486, 2126
TseI	GCWGC	4 cut(s)	317, 723, 823, 2061
Tsp45I	GTSAC	3 cut(s)	1121, 1486, 2126
TspGWI	ACGGA	9 cut(s)	19, 34, 64, 425, 1513, 1532, 1664, 1862, 2226
TspRI	CASTG	8 cut(s)	631, 889, 1491, 1906, 1967, 1975, 2104, 2158
Van91I	CCANNNNNTGG	1 cut(s)	1490
VpaK11BI	GGWCC	4 cut(s)	7, 1381, 1391, 1728
XagI	CCTNNNNNAGG	2 cut(s)	1005, 1174
XapI	RAATTY	5 cut(s)	17, 192, 874, 933, 1570
XbaI	TCTAGA	2 cut(s)	2135, 2243
XceI	RCATGY	3 cut(s)	248, 653, 1417
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2072
XmnI	GAANNNNTTC	1 cut(s)	1558
XspI	CTAG	6 cut(s)	207, 638, 863, 2136, 2244, 2263
Zsp2I	ATGCAT	1 cut(s)	752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.