MD08G1092400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013625
Physical Location & Seq
Forward (+)
7699490 .. 7700523
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1092400.v1.1.491

Sequence Viewer

Length: 267 bp
ATGGATGCTTCGAAGGGATCAATGGCAAAAACAAAAGAGGAAATCAGGGTGGAGACGGTGGATTATCGATCACCGGCGGGAGAAGACAAAGAAGCCAAGAAGGAGGACGTTGAAGTGGTTCATGAGATTCGAGACGGCGGAGCAAAGAGTGAGGGTGTTCACCCTTTTGCAGGTGCAGCTGCTGCAGTGGCAAACAGCATTCAATCAGCAAAGGATGCCATCTCTGGTGGTGCCAAAGAATCCAAAGATGATAAGACCAGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

9.21

Weight (kDa)

5.38

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 230
AciI CCGC 2 cut(s) 77, 138
AclWI GGATC 1 cut(s) 25
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 2 cut(s) 113, 203
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
Alw26I GTCTC 2 cut(s) 47, 126
AlwI GGATC 1 cut(s) 25
AlwNI CAGNNNCTG 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 176, 179, 182
Asp700I GAANNNNTTC 1 cut(s) 117
AsuHPI GGTGA 2 cut(s) 63, 152
AsuII TTCGAA 1 cut(s) 11
BanI GGYRCC 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 166, 169, 188
BccI CCATC 1 cut(s) 227
BceAI ACGGC 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 47, 126
BfmI CTRYAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 3 cut(s) 177, 180, 183
BlsI GCNGC 3 cut(s) 178, 181, 184
BmiI GGNNCC 1 cut(s) 232
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 90
Bpu14I TTCGAA 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 67
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 258
BseCI ATCGAT 1 cut(s) 67
BseGI GGATG 2 cut(s) 10, 220
BseLI CCNNNNNNNGG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 258
BseXI GCAGC 3 cut(s) 166, 169, 188
BsgI GTGCAG 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 67
BsiSI CCGG 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 47, 126
BsmBI CGTCTC 2 cut(s) 47, 126
BsmI GAATGC 1 cut(s) 198
Bsp119I TTCGAA 1 cut(s) 11
Bsp143I GATC 2 cut(s) 17, 68
BspACI CCGC 2 cut(s) 77, 138
BspDI ATCGAT 1 cut(s) 67
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 1 cut(s) 25
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 1 cut(s) 258
BssAI RCCGGY 1 cut(s) 73
BssMI GATC 2 cut(s) 17, 68
Bst4CI ACNGT 1 cut(s) 58
BstAPI GCANNNNNTGC 2 cut(s) 182, 215
BstBI TTCGAA 1 cut(s) 11
BstENI CCTNNNNNAGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 10, 220
BstKTI GATC 2 cut(s) 20, 71
BstMAI GTCTC 2 cut(s) 47, 126
BstMBI GATC 2 cut(s) 17, 68
BstMWI GCNNNNNNNGC 4 cut(s) 176, 182, 188, 215
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 166, 169, 188
BstV2I GAAGAC 1 cut(s) 90
Bsu15I ATCGAT 1 cut(s) 67
BsuTUI ATCGAT 1 cut(s) 67
BtsCI GGATG 2 cut(s) 10, 220
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 192
BveI ACCTGC 1 cut(s) 161
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 73
ClaI ATCGAT 1 cut(s) 67
CviAII CATG 1 cut(s) 122
CviJI RGCY 2 cut(s) 95, 179
CviKI_1 RGCY 2 cut(s) 95, 179
DpnI GATC 2 cut(s) 19, 70
DpnII GATC 2 cut(s) 17, 68
EciI GGCGGA 1 cut(s) 153
EcoNI CCTNNNNNAGG 1 cut(s) 168
Esp3I CGTCTC 2 cut(s) 47, 126
FaeI CATG 1 cut(s) 125
FaiI YATR 1 cut(s) 123
FatI CATG 1 cut(s) 121
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 3 cut(s) 177, 180, 183
FokI GGATG 2 cut(s) 17, 227
Fsp4HI GCNGC 3 cut(s) 177, 180, 183
GluI GCNGC 3 cut(s) 177, 180, 183
HapII CCGG 1 cut(s) 74
Hin1II CATG 1 cut(s) 125
HinfI GANTC 2 cut(s) 127, 239
HpaII CCGG 1 cut(s) 74
HphI GGTGA 2 cut(s) 63, 152
Hpy166II GTNNAC 1 cut(s) 160
Hpy188III TCNNGA 2 cut(s) 122, 131
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 7, 94
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 170, 176, 185
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 182, 188, 215
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 1 cut(s) 125
Kzo9I GATC 2 cut(s) 17, 68
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 4 cut(s) 31, 87, 156, 210
Lsp1109I GCAGC 3 cut(s) 166, 169, 188
LweI GCATC 1 cut(s) 205
MaeII ACGT 1 cut(s) 108
MalI GATC 2 cut(s) 19, 70
MboI GATC 2 cut(s) 17, 68
MboII GAAGA 1 cut(s) 95
MnlI CCTC 3 cut(s) 31, 97, 145
MroXI GAANNNNTTC 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 74
Mva1269I GAATGC 1 cut(s) 198
MwoI GCNNNNNNNGC 4 cut(s) 176, 182, 188, 215
NdeII GATC 2 cut(s) 17, 68
NlaIII CATG 1 cut(s) 125
NlaIV GGNNCC 1 cut(s) 232
NspV TTCGAA 1 cut(s) 11
PagI TCATGA 1 cut(s) 121
PaqCI CACCTGC 1 cut(s) 161
PctI GAATGC 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 127, 239
PkrI GCNGC 3 cut(s) 178, 181, 184
PspN4I GGNNCC 1 cut(s) 232
PstI CTGCAG 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 179
SatI GCNGC 3 cut(s) 177, 180, 183
Sau3AI GATC 2 cut(s) 17, 68
SetI ASST 3 cut(s) 111, 175, 181
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 183
SfuI TTCGAA 1 cut(s) 11
SgeI CNNG 8 cut(s) 58, 86, 90, 109, 134, 143, 183, 237
SgrAI CRCCGGYG 1 cut(s) 73
SsiI CCGC 2 cut(s) 77, 138
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 111
TaqI TCGA 3 cut(s) 11, 67, 130
TfiI GAWTC 2 cut(s) 127, 239
TscAI CASTG 1 cut(s) 192
TseI GCWGC 3 cut(s) 176, 179, 182
TspDTI ATGAA 1 cut(s) 110
TspRI CASTG 1 cut(s) 192
XagI CCTNNNNNAGG 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.