MD08G1096300.v1.1

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012231
Physical Location & Seq
Forward (+)
8081170 .. 8082937
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1096300.v1.1.491

Sequence Viewer

Length: 990 bp
ATGCATACATGTCTCTCTTTCTCTCTCTTTCTCTCTCTCACACACAAACACAACATTCTTTTTTTTCTTGCTTTATCTTTTCATATTTTTAGGTGGAAAATCCCAAGTCAACCTCACCTCATCATTTGGTCTTGGTTGTTCCAAACACCACCTCATTTCCCACTTTGTCACTTTAATAACTGTGATCATGATCTTCGTTTTTGGCCCTCTTATATAATTACTTGCTATCTAGCTCTAAGGCTCTATCATTCAGTACTCCACTCACCACATATGGGAATGAGCAGCATGAAGGTGCATCAGTTCGCACGTGGACTCTGGGAGCACGAACCCTCCCTTACGCTTGGCTGCACCAAACGCTTACGCCCTCTTGCTCCCAAACTGGCCAACACCGCGGCCGCCACCTCCGACACTACTACCACTCTCGCTCCTTTCGATCTCAAGAGTTTCATCAGACCTGAAAGTGGCCCCAGAAAACTTGGATCTTCTGACCATCTTCAGGAGAAGAAAGATTCCCCCCACCCTCAGGTAGAGACGCACCCGGGAGGGACACGCTGGAACCCTACGCAAGAACAGATAGGGATACTTGAAATGTTGTATAGAGGAGGAATGCGCACTCCTAATGCACAACAAATAGAGCAAATCACGGCTCAACTTGGCAAGTACGGCAAGATCGAAGGAAAGAATGTGTTTTACTGGTTCCAGAATCACAAGGCACGTGAGAGGCAGAAGCAAAAGCGCAGCAGCCTCGGCCTCAGCCACTCTCCAAGAACACCGACTCCTGTAATTACCACCCCGGGAGAAATAGTAGTGGAAAGAGAGGAAGATAGTCCGTACAAGAGAAAGTGTAGGAGCTGGGGATTTGATTGCTTGGTAGAGGGTGACCATGGTAGTGTAGTATGTAAGGTCGACAACGACGACGCCGTTAAGGGAGGTAGTGTTGGAGTTATGACTCTGGAACTCTTCCCATTACATCCAGAAGGAAGAAAATGA

Protein Analysis

330

Amino Acids

37.59

Weight (kDa)

9.23

Isoelectric Point (pI)

57.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 183 - 242 1.9e-14 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46480 AT1G46480
fragaria_vesca FvH4_2g34030 FvH4_2g34030
malus_domestica MD08G1096300.v1.1 MD15G1079000.v1.1
prunus_persica Prupe.1G432100_v2.0.a1
pyrus_communis pycom08g07830 pycom15g07360
rosa_chinensis RchiOBHm_Chr6g0308351
rosa_laevigata RLG00000010648
rosa_multiflora Rmu_sc0005523.1_g000011
rosa_roxburghii Rroxscaffold_7G00160220
rosa_rugosa Rorug06G0366200
rosa_samantha Rh6AG475500 Rh6BG485900 Rh6CG491100 Rh6DG477400
rosa_wichuraiana Rw6G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 611
AccI GTMKAC 1 cut(s) 906
AccII CGCG 1 cut(s) 392
AciI CCGC 3 cut(s) 390, 392, 396
AclWI GGATC 1 cut(s) 487
AcoI YGGCCR 2 cut(s) 381, 393
AcuI CTGAAG 1 cut(s) 479
AcvI CACGTG 2 cut(s) 308, 716
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 3 cut(s) 255, 662, 833
AfiI CCNNNNNNNGG 4 cut(s) 272, 461, 496, 523
AflIII ACRYGT 1 cut(s) 8
AgsI TTSAA 1 cut(s) 587
AloI GAACNNNNNNTCC 2 cut(s) 760, 792
AluBI AGCT 2 cut(s) 233, 852
AluI AGCT 2 cut(s) 233, 852
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 2 cut(s) 17, 524
AlwI GGATC 1 cut(s) 487
Ama87I CYCGRG 2 cut(s) 538, 793
AoxI GGCC 5 cut(s) 203, 381, 393, 463, 748
ApeKI GCWGC 4 cut(s) 282, 345, 738, 741
AspLEI GCGC 2 cut(s) 612, 738
AspS9I GGNCC 2 cut(s) 204, 464
AsuC2I CCSGG 4 cut(s) 539, 540, 794, 795
AsuHPI GGTGA 3 cut(s) 107, 255, 890
AvaI CYCGRG 2 cut(s) 538, 793
AxyI CCTNAGG 1 cut(s) 522
BalI TGGCCA 1 cut(s) 383
BbrPI CACGTG 2 cut(s) 308, 716
Bbv12I GWGCWC 1 cut(s) 324
BbvCI CCTCAGC 1 cut(s) 752
BbvI GCAGC 4 cut(s) 294, 332, 750, 753
BccI CCATC 1 cut(s) 498
BceAI ACGGC 3 cut(s) 660, 679, 905
BcgI CGANNNNNNTGC 2 cut(s) 727, 761
BciVI GTATCC 1 cut(s) 573
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 4 cut(s) 539, 540, 794, 795
BcoDI GTCTC 2 cut(s) 17, 524
BfaI CTAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 573
BisI GCNGC 6 cut(s) 283, 346, 393, 396, 739, 742
BlsI GCNGC 6 cut(s) 284, 347, 394, 397, 740, 743
BmcAI AGTACT 1 cut(s) 255
Bme1390I CCNGG 4 cut(s) 539, 540, 794, 795
BmeT110I CYCGRG 2 cut(s) 538, 793
BmgT120I GGNCC 2 cut(s) 204, 464
BmiI GGNNCC 3 cut(s) 466, 557, 698
BmrFI CCNGG 4 cut(s) 539, 540, 794, 795
BmsI GCATC 1 cut(s) 304
Bpu10I CCTNAGC 1 cut(s) 752
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 4 cut(s) 539, 540, 794, 795
BsaAI YACGTR 2 cut(s) 308, 716
BsaBI GATNNNNATC 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 6 cut(s) 390, 538, 745, 792, 793, 883
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 461, 496, 523
Bse1I ACTGG 2 cut(s) 384, 698
Bse21I CCTNAGG 1 cut(s) 522
Bse8I GATNNNNATC 1 cut(s) 189
BseDI CCNNGG 6 cut(s) 390, 538, 745, 792, 793, 883
BseGI GGATG 1 cut(s) 970
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 4 cut(s) 272, 461, 496, 523
BseMII CTCAG 2 cut(s) 536, 766
BseNI ACTGG 2 cut(s) 384, 698
BseRI GAGGAG 1 cut(s) 615
BseX3I CGGCCG 1 cut(s) 393
BseXI GCAGC 4 cut(s) 294, 332, 750, 753
BseYI CCCAGC 1 cut(s) 852
BsgI GTGCAG 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 5 cut(s) 205, 383, 395, 465, 750
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 324
BsiHKCI CYCGRG 2 cut(s) 538, 793
BsiSI CCGG 2 cut(s) 539, 794
BslFI GGGAC 1 cut(s) 559
BslI CCNNNNNNNGG 4 cut(s) 272, 461, 496, 523
BsmAI GTCTC 2 cut(s) 17, 524
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 1 cut(s) 559
BsmI GAATGC 1 cut(s) 612
BsnI GGCC 5 cut(s) 205, 383, 395, 465, 750
BsoBI CYCGRG 2 cut(s) 538, 793
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 5 cut(s) 184, 190, 433, 479, 669
Bsp19I CCATGG 1 cut(s) 883
BspACI CCGC 3 cut(s) 390, 392, 396
BspANI GGCC 5 cut(s) 205, 383, 395, 465, 750
BspCNI CTCAG 2 cut(s) 535, 765
BspFNI CGCG 1 cut(s) 392
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 3 cut(s) 466, 557, 698
BspPI GGATC 1 cut(s) 487
BsrI ACTGG 2 cut(s) 384, 698
BssECI CCNNGG 6 cut(s) 390, 538, 745, 792, 793, 883
BssMI GATC 5 cut(s) 184, 190, 433, 479, 669
BssNI GRCGYC 1 cut(s) 918
BssT1I CCWWGG 1 cut(s) 883
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 965
BstACI GRCGYC 1 cut(s) 918
BstBAI YACGTR 2 cut(s) 308, 716
BstDEI CTNAG 3 cut(s) 236, 522, 752
BstDSI CCRYGG 2 cut(s) 390, 883
BstEII GGTNACC 1 cut(s) 878
BstF5I GGATG 1 cut(s) 970
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 2 cut(s) 612, 738
BstKTI GATC 5 cut(s) 187, 193, 436, 482, 672
BstMAI GTCTC 2 cut(s) 17, 524
BstMBI GATC 5 cut(s) 184, 190, 433, 479, 669
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 4 cut(s) 354, 389, 663, 747
BstNSI RCATGY 1 cut(s) 12
BstPI GGTNACC 1 cut(s) 878
BstSCI CCNGG 4 cut(s) 537, 538, 792, 793
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 4 cut(s) 294, 332, 750, 753
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BstZI CGGCCG 1 cut(s) 393
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 573
BsuRI GGCC 5 cut(s) 205, 383, 395, 465, 750
BtgI CCRYGG 2 cut(s) 390, 883
BtsCI GGATG 1 cut(s) 970
CciI TCATGA 1 cut(s) 187
CciNI GCGGCCGC 1 cut(s) 393
CfoI GCGC 2 cut(s) 612, 738
Cfr13I GGNCC 2 cut(s) 204, 464
Cfr42I CCGCGG 1 cut(s) 393
Cfr9I CCCGGG 2 cut(s) 538, 793
CseI GACGC 2 cut(s) 541, 926
Csp6I GTAC 3 cut(s) 254, 661, 832
CviAII CATG 4 cut(s) 9, 188, 286, 884
CviQI GTAC 3 cut(s) 254, 661, 832
DdeI CTNAG 3 cut(s) 236, 522, 752
DpnI GATC 5 cut(s) 186, 192, 435, 481, 671
DpnII GATC 5 cut(s) 184, 190, 433, 479, 669
EaeI YGGCCR 2 cut(s) 381, 393
EagI CGGCCG 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 965
EarI CTCTTC 1 cut(s) 965
EclXI CGGCCG 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 883
Eco52I CGGCCG 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 479
Eco72I CACGTG 2 cut(s) 308, 716
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 2 cut(s) 538, 793
Eco91I GGTNACC 1 cut(s) 878
EcoO65I GGTNACC 1 cut(s) 878
EcoT14I CCWWGG 1 cut(s) 883
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 883
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 4 cut(s) 12, 191, 289, 887
FaqI GGGAC 1 cut(s) 559
FatI CATG 4 cut(s) 8, 187, 285, 883
FauNDI CATATG 1 cut(s) 270
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 906
Fnu4HI GCNGC 6 cut(s) 283, 346, 393, 396, 739, 742
FokI GGATG 1 cut(s) 957
Fsp4HI GCNGC 6 cut(s) 283, 346, 393, 396, 739, 742
FspAI RTGCGCAY 1 cut(s) 611
FspBI CTAG 1 cut(s) 230
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 2 cut(s) 611, 737
GluI GCNGC 6 cut(s) 283, 346, 393, 396, 739, 742
GsaI CCCAGC 1 cut(s) 856
HaeIII GGCC 5 cut(s) 205, 383, 395, 465, 750
HapII CCGG 2 cut(s) 539, 794
HgaI GACGC 2 cut(s) 541, 926
HhaI GCGC 2 cut(s) 612, 738
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 4 cut(s) 12, 191, 289, 887
Hin6I GCGC 2 cut(s) 610, 736
HinP1I GCGC 2 cut(s) 610, 736
HincII GTYRAC 2 cut(s) 110, 907
HindII GTYRAC 2 cut(s) 110, 907
HinfI GANTC 5 cut(s) 312, 509, 703, 775, 949
HpaII CCGG 2 cut(s) 539, 794
HphI GGTGA 3 cut(s) 107, 255, 890
Hpy166II GTNNAC 3 cut(s) 110, 311, 907
Hpy188I TCNGA 3 cut(s) 406, 452, 487
Hpy188III TCNNGA 6 cut(s) 188, 439, 497, 700, 953, 974
Hpy8I GTNNAC 3 cut(s) 110, 311, 907
Hpy99I CGWCG 2 cut(s) 917, 920
HpyAV CCTTC 3 cut(s) 283, 668, 971
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 2 cut(s) 307, 715
HpyCH4V TGCA 4 cut(s) 4, 295, 348, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 354, 389, 663, 747
HpyF3I CTNAG 3 cut(s) 236, 522, 752
HpySE526I ACGT 2 cut(s) 307, 715
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 4 cut(s) 12, 191, 289, 887
HspAI GCGC 2 cut(s) 610, 736
Ksp22I TGATCA 1 cut(s) 184
KspI CCGCGG 1 cut(s) 393
Kzo9I GATC 5 cut(s) 184, 190, 433, 479, 669
LmnI GCTCC 4 cut(s) 319, 376, 430, 849
Lsp1109I GCAGC 4 cut(s) 294, 332, 750, 753
LweI GCATC 1 cut(s) 304
MaeI CTAG 1 cut(s) 230
MaeII ACGT 2 cut(s) 307, 715
MaeIII GTNAC 2 cut(s) 167, 878
MalI GATC 5 cut(s) 186, 192, 435, 481, 671
MboI GATC 5 cut(s) 184, 190, 433, 479, 669
MboII GAAGA 6 cut(s) 185, 474, 485, 514, 833, 952
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 1 cut(s) 324
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 2 cut(s) 216, 783
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 3 cut(s) 306, 769, 943
MmeI TCCRAC 2 cut(s) 429, 919
Mox20I TGGCCA 1 cut(s) 383
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 383
MseI TTAA 2 cut(s) 174, 924
MslI CAYNNNNRTG 2 cut(s) 290, 888
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 2 cut(s) 539, 794
MspR9I CCNGG 4 cut(s) 539, 540, 794, 795
Mva1269I GAATGC 1 cut(s) 612
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 4 cut(s) 354, 389, 663, 747
NciI CCSGG 4 cut(s) 539, 540, 794, 795
NcoI CCATGG 1 cut(s) 883
NdeI CATATG 1 cut(s) 270
NdeII GATC 5 cut(s) 184, 190, 433, 479, 669
NlaIII CATG 4 cut(s) 12, 191, 289, 887
NlaIV GGNNCC 3 cut(s) 466, 557, 698
NmeAIII GCCGAG 1 cut(s) 726
NmuCI GTSAC 2 cut(s) 167, 878
NotI GCGGCCGC 1 cut(s) 393
NsbI TGCGCA 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 12
PagI TCATGA 1 cut(s) 187
PciI ACATGT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 4 cut(s) 429, 669, 912, 918
PctI GAATGC 1 cut(s) 612
PfeI GAWTC 2 cut(s) 509, 703
PkrI GCNGC 6 cut(s) 284, 347, 394, 397, 740, 743
PleI GAGTC 3 cut(s) 306, 769, 943
PmaCI CACGTG 2 cut(s) 308, 716
PmlI CACGTG 2 cut(s) 308, 716
PpsI GAGTC 3 cut(s) 306, 769, 943
Ppu21I YACGTR 2 cut(s) 308, 716
PscI ACATGT 1 cut(s) 8
PspCI CACGTG 2 cut(s) 308, 716
PspEI GGTNACC 1 cut(s) 878
PspFI CCCAGC 1 cut(s) 852
PspN4I GGNNCC 3 cut(s) 466, 557, 698
PspPI GGNCC 2 cut(s) 204, 464
PsuI RGATCY 1 cut(s) 479
RsaI GTAC 3 cut(s) 255, 662, 833
RsaNI GTAC 3 cut(s) 254, 661, 832
RseI CAYNNNNRTG 2 cut(s) 290, 888
SacII CCGCGG 1 cut(s) 393
SalI GTCGAC 1 cut(s) 905
SaqAI TTAA 2 cut(s) 174, 924
SatI GCNGC 6 cut(s) 283, 346, 393, 396, 739, 742
Sau3AI GATC 5 cut(s) 184, 190, 433, 479, 669
Sau96I GGNCC 2 cut(s) 204, 464
ScaI AGTACT 1 cut(s) 255
SchI GAGTC 3 cut(s) 306, 769, 943
ScrFI CCNGG 4 cut(s) 539, 540, 794, 795
SduI GDGCHC 1 cut(s) 324
SfaNI GCATC 1 cut(s) 304
Sfr303I CCGCGG 1 cut(s) 393
SgrBI CCGCGG 1 cut(s) 393
SmaI CCCGGG 2 cut(s) 540, 795
SmiMI CAYNNNNRTG 2 cut(s) 290, 888
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 2 cut(s) 216, 783
SsiI CCGC 3 cut(s) 390, 392, 396
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 4 cut(s) 537, 538, 792, 793
StyI CCWWGG 1 cut(s) 883
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 2 cut(s) 310, 718
TaqI TCGA 3 cut(s) 432, 672, 906
TasI AATT 2 cut(s) 216, 783
TatI WGTACW 1 cut(s) 253
TauI GCSGC 2 cut(s) 395, 398
TfiI GAWTC 2 cut(s) 509, 703
Tru1I TTAA 2 cut(s) 174, 924
Tru9I TTAA 2 cut(s) 174, 924
TseFI GTSAC 2 cut(s) 167, 878
TseI GCWGC 4 cut(s) 282, 345, 738, 741
Tsp45I GTSAC 2 cut(s) 167, 878
TspDTI ATGAA 3 cut(s) 71, 302, 436
TspGWI ACGGA 1 cut(s) 819
TspMI CCCGGG 2 cut(s) 538, 793
XceI RCATGY 1 cut(s) 12
XmaI CCCGGG 2 cut(s) 538, 793
XmiI GTMKAC 1 cut(s) 906
XspI CTAG 1 cut(s) 230
ZrmI AGTACT 1 cut(s) 255
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.