RLG00000010648

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012231
Physical Location & Seq
Forward (+)
3294103 .. 3295084
982 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010648

Sequence Viewer

Length: 690 bp
ATGGGAATGAGCAGCATGAAGGTGCATCAGTTCGCACGTGGACTCTGGGACTCATCCACCACCAATCTTGAACCCTCCCTCAGCCTTGGATGCAAGCGCTTACGCCCTCTTGCTCCCAAACTCTCCGACACTACTGCAACCACTACCCTCGCTCCTTTCGATCTCAAGAGCTTCATCAGACCCGAAAGTGGCCCTAGAAACAAGCTAGCATCTCCTCATGACCATGAGAAGAAAGATTCCTCCCCCTCCCAGGTAGAGATGCACCCGGGAGGGACGCGTTGGAATCCAACACAAGAGCAGATAGGGATATTGGAAATGCTATATAGAGGAGGAATGCGGACTCCTAATGCTCAGCAGATAGAGCAAATCACTGCTCAGCTTGGCAAGTACGGCAAGATTGAAGGCAAGAATGTGTTCTACTGGTTCCAAAATCACAAAGCACGCGAAAGACAGAAGCAGAAACGCAGCACTCTTGGTCTCTCCCACTCTCCAAGAACCCCGACTCCTGTAATTACCACCACCGGGGAAGTACTGGAAAGAGAGGAAGACAGTCCATACAAGAGAAAGTGCAGGAGCTGGGCATTTGATTGTTTGGGAGACGATTCTAGTACTATAACTTGTAACAAAGACGTCGTTGAGGGAGATAGAACTCTGGAGCTCTTCCCATTACGCCCAGAAGGCATGAGATGA

Protein Analysis

230

Amino Acids

25.89

Weight (kDa)

9.3

Isoelectric Point (pI)

63.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 92 - 151 1e-14 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46480 AT1G46480
fragaria_vesca FvH4_2g34030 FvH4_2g34030
malus_domestica MD08G1096300.v1.1 MD15G1079000.v1.1
prunus_persica Prupe.1G432100_v2.0.a1
pyrus_communis pycom08g07830 pycom15g07360
rosa_chinensis RchiOBHm_Chr6g0308351
rosa_laevigata RLG00000010648
rosa_multiflora Rmu_sc0005523.1_g000011
rosa_roxburghii Rroxscaffold_7G00160220
rosa_rugosa Rorug06G0366200
rosa_samantha Rh6AG475500 Rh6BG485900 Rh6CG491100 Rh6DG477400
rosa_wichuraiana Rw6G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 633
AccII CGCG 2 cut(s) 277, 444
AciI CCGC 1 cut(s) 337
AcvI CACGTG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 3 cut(s) 389, 531, 610
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 3 cut(s) 188, 250, 522
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 2 cut(s) 71, 401
AjnI CCWGG 1 cut(s) 249
AloI GAACNNNNNNTCC 2 cut(s) 487, 519
AluBI AGCT 5 cut(s) 171, 205, 379, 576, 658
AluI AGCT 5 cut(s) 171, 205, 379, 576, 658
Alw21I GWGCWC 1 cut(s) 660
Alw26I GTCTC 2 cut(s) 482, 591
AlwNI CAGNNNCTG 1 cut(s) 576
Ama87I CYCGRG 1 cut(s) 265
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 12, 465
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 191
AsuC2I CCSGG 3 cut(s) 266, 267, 523
AsuNHI GCTAGC 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 265
BanII GRGCYC 1 cut(s) 660
BbrPI CACGTG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 660
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 24, 477
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 3 cut(s) 266, 267, 523
BcoDI GTCTC 2 cut(s) 482, 591
BfaI CTAG 3 cut(s) 195, 206, 606
BfoI RGCGCY 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 678
BisI GCNGC 2 cut(s) 13, 466
BlpI GCTNAGC 2 cut(s) 351, 375
BlsI GCNGC 2 cut(s) 14, 467
BmcAI AGTACT 2 cut(s) 531, 610
Bme1390I CCNGG 4 cut(s) 251, 266, 267, 523
BmeT110I CYCGRG 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 425
BmrFI CCNGG 4 cut(s) 251, 266, 267, 523
BmsI GCATC 4 cut(s) 34, 80, 218, 249
BmtI GCTAGC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 552
BpmI CTGGAG 1 cut(s) 674
Bpu10I CCTNAGC 1 cut(s) 80
Bpu1102I GCTNAGC 2 cut(s) 351, 375
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 3 cut(s) 266, 267, 523
BsaAI YACGTR 1 cut(s) 38
BsaHI GRCGYC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 4 cut(s) 85, 249, 265, 522
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 487, 517
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 250, 522
Bse1I ACTGG 2 cut(s) 425, 537
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 4 cut(s) 85, 249, 265, 522
BseGI GGATG 2 cut(s) 53, 95
BseLI CCNNNNNNNGG 3 cut(s) 188, 250, 522
BseMII CTCAG 3 cut(s) 94, 365, 389
BseNI ACTGG 2 cut(s) 425, 537
BseRI GAGGAG 2 cut(s) 204, 342
BseXI GCAGC 2 cut(s) 24, 477
BseYI CCCAGC 1 cut(s) 576
BsgI GTGCAG 1 cut(s) 589
Bsh1236I CGCG 2 cut(s) 277, 444
BshFI GGCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 660
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 2 cut(s) 266, 522
BslFI GGGAC 2 cut(s) 62, 286
BslI CCNNNNNNNGG 3 cut(s) 188, 250, 522
BsmAI GTCTC 2 cut(s) 482, 591
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 2 cut(s) 62, 286
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 1 cut(s) 192
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 1 cut(s) 160
Bsp1720I GCTNAGC 2 cut(s) 351, 375
BspACI CCGC 1 cut(s) 337
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 3 cut(s) 93, 364, 388
BspFNI CGCG 2 cut(s) 277, 444
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 425
BspOI GCTAGC 1 cut(s) 209
BspQI GCTCTTC 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 482
BsrI ACTGG 2 cut(s) 425, 537
BssECI CCNNGG 4 cut(s) 85, 249, 265, 522
BssMI GATC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 665
BstACI GRCGYC 1 cut(s) 630
BstBAI YACGTR 1 cut(s) 38
BstC8I GCNNGC 3 cut(s) 95, 207, 442
BstDEI CTNAG 3 cut(s) 80, 351, 375
BstF5I GGATG 2 cut(s) 53, 95
BstFNI CGCG 2 cut(s) 277, 444
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 2 cut(s) 482, 591
BstMBI GATC 1 cut(s) 160
BstMWI GCNNNNNNNGC 4 cut(s) 90, 361, 390, 678
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 4 cut(s) 249, 264, 265, 521
BstUI CGCG 2 cut(s) 277, 444
BstV1I GCAGC 2 cut(s) 24, 477
BstV2I GAAGAC 1 cut(s) 552
BsuRI GGCC 1 cut(s) 192
BtsCI GGATG 2 cut(s) 53, 95
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 95, 207, 442
CaiI CAGNNNCTG 1 cut(s) 576
CciI TCATGA 1 cut(s) 217
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 1 cut(s) 191
Cfr9I CCCGGG 1 cut(s) 265
CseI GACGC 1 cut(s) 283
Csp6I GTAC 3 cut(s) 388, 530, 609
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 4 cut(s) 16, 218, 224, 682
CviJI RGCY 7 cut(s) 84, 171, 192, 205, 379, 576, 658
CviKI_1 RGCY 7 cut(s) 84, 171, 192, 205, 379, 576, 658
CviQI GTAC 3 cut(s) 388, 530, 609
DdeI CTNAG 3 cut(s) 80, 351, 375
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 665
EarI CTCTTC 1 cut(s) 665
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 482
Eco47III AGCGCT 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 658
Eco72I CACGTG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 4 cut(s) 19, 221, 227, 685
FaiI YATR 8 cut(s) 17, 219, 225, 322, 324, 556, 614, 683
FaqI GGGAC 2 cut(s) 62, 286
FatI CATG 4 cut(s) 15, 217, 223, 681
Fnu4HI GCNGC 2 cut(s) 13, 466
FokI GGATG 2 cut(s) 40, 102
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 2 cut(s) 13, 466
FspBI CTAG 3 cut(s) 195, 206, 606
GlaI GCGC 1 cut(s) 98
GluI GCNGC 2 cut(s) 13, 466
GsaI CCCAGC 1 cut(s) 580
GsuI CTGGAG 1 cut(s) 674
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 1 cut(s) 192
HapII CCGG 2 cut(s) 266, 522
HgaI GACGC 1 cut(s) 283
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 4 cut(s) 19, 221, 227, 685
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 7 cut(s) 42, 50, 236, 283, 340, 502, 602
HpaII CCGG 2 cut(s) 266, 522
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 2 cut(s) 127, 179
Hpy188III TCNNGA 4 cut(s) 68, 166, 218, 653
Hpy8I GTNNAC 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 3 cut(s) 13, 395, 671
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 2 cut(s) 37, 630
HpyCH4V TGCA 5 cut(s) 25, 93, 137, 262, 570
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 361, 390, 678
HpyF3I CTNAG 3 cut(s) 80, 351, 375
HpySE526I ACGT 2 cut(s) 37, 630
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 4 cut(s) 19, 221, 227, 685
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 1 cut(s) 160
LguI GCTCTTC 1 cut(s) 665
LmnI GCTCC 4 cut(s) 118, 157, 573, 655
Lsp1109I GCAGC 2 cut(s) 24, 477
LweI GCATC 4 cut(s) 34, 80, 218, 249
MaeI CTAG 3 cut(s) 195, 206, 606
MaeII ACGT 2 cut(s) 37, 630
MaeIII GTNAC 1 cut(s) 620
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 3 cut(s) 241, 557, 652
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 1 cut(s) 510
MluI ACGCGT 1 cut(s) 275
MlyI GAGTC 4 cut(s) 36, 44, 334, 496
MmeI TCCRAC 3 cut(s) 150, 260, 311
MroXI GAANNNNTTC 1 cut(s) 413
MslI CAYNNNNRTG 2 cut(s) 20, 222
MspI CCGG 2 cut(s) 266, 522
MspR9I CCNGG 4 cut(s) 251, 266, 267, 523
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 2 cut(s) 277, 444
MwoI GCNNNNNNNGC 4 cut(s) 90, 361, 390, 678
NciI CCSGG 3 cut(s) 266, 267, 523
NdeII GATC 1 cut(s) 160
NheI GCTAGC 1 cut(s) 205
NlaIII CATG 4 cut(s) 19, 221, 227, 685
NlaIV GGNNCC 1 cut(s) 425
PagI TCATGA 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 665
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 3 cut(s) 236, 283, 602
PkrI GCNGC 2 cut(s) 14, 467
PleI GAGTC 4 cut(s) 36, 44, 334, 496
PmaCI CACGTG 1 cut(s) 38
PmlI CACGTG 1 cut(s) 38
PpsI GAGTC 4 cut(s) 36, 44, 334, 496
Ppu21I YACGTR 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 249
PspCI CACGTG 1 cut(s) 38
PspFI CCCAGC 1 cut(s) 576
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 425
PspPI GGNCC 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 576
RsaI GTAC 3 cut(s) 389, 531, 610
RsaNI GTAC 3 cut(s) 388, 530, 609
RseI CAYNNNNRTG 2 cut(s) 20, 222
SacI GAGCTC 1 cut(s) 660
SapI GCTCTTC 1 cut(s) 665
SatI GCNGC 2 cut(s) 13, 466
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 1 cut(s) 191
ScaI AGTACT 2 cut(s) 531, 610
SchI GAGTC 4 cut(s) 36, 44, 334, 496
ScrFI CCNGG 4 cut(s) 251, 266, 267, 523
SduI GDGCHC 1 cut(s) 660
SetI ASST 9 cut(s) 24, 40, 173, 207, 255, 381, 578, 633, 660
SfaNI GCATC 4 cut(s) 34, 80, 218, 249
SmaI CCCGGG 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 20, 222
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 1 cut(s) 510
SsiI CCGC 1 cut(s) 337
SspMI CTAG 3 cut(s) 195, 206, 606
SstI GAGCTC 1 cut(s) 660
StyD4I CCNGG 4 cut(s) 249, 264, 265, 521
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 2 cut(s) 40, 633
TaqI TCGA 1 cut(s) 159
TasI AATT 1 cut(s) 510
TatI WGTACW 2 cut(s) 529, 608
TfiI GAWTC 3 cut(s) 236, 283, 602
TscAI CASTG 1 cut(s) 376
TseI GCWGC 2 cut(s) 12, 465
TspDTI ATGAA 2 cut(s) 32, 163
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 1 cut(s) 376
XmaI CCCGGG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 3 cut(s) 195, 206, 606
ZraI GACGTC 1 cut(s) 631
ZrmI AGTACT 2 cut(s) 531, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.