Rh6AG475500

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012231
Physical Location & Seq
Reverse (-)
65708090 .. 65710760
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG475500.1

Sequence Viewer

Length: 609 bp
ATGGGAATGAGCAGCATGAAGGTGCATCAGTTCGCACGTGGACTCTGGGACTCATCCACCACCAATCTTGAACCCTCCCTCAGCCTTGGATGCAAGCGCTTACGCCCTCTTGCTCCCAAACTCTCCGACACTACTGCAACCACTACTCTCGCTCCTTTCGATCTCAAGAGCTTCATCAGACCCGAAAGTGGCCCTAGAAACAAGCTAGCATCTCCTCATGACCATGAGAAGAAAGATTCCTCCCCCTCCCAGGTAGAGATGCACCCGGGAGGGACGCGTTGGAATCCAACACAAGAGCAGATAGGGATATTGGAAATGCTGTATAGAGGAGGAATGCGGACTCCGAATGCTCAGCAGATAGAGCAAATCACTGCTCAGCTTGGCAAGTACGGCAAGATTGAAGGCAAGAATGTGTTCTACTGGTTCCAAAATCACAAAGCACGCGAAAGACAAAAGCAGAAACGCAGCACTCTTGGTCTCACCCACTCTCCAAGAACCCCAACTCCTGTAATTACCACCACCGTAAGTGCTCCCTACAGCGCAGTAATTAGCTCATGTTCTTTTGTAGTTTTACTTGATCGATCGCTTATCAAACTGGGTTACATGTAA

Protein Analysis

202

Amino Acids

22.57

Weight (kDa)

9.96

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 92 - 151 8.2e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46480 AT1G46480
fragaria_vesca FvH4_2g34030 FvH4_2g34030
malus_domestica MD08G1096300.v1.1 MD15G1079000.v1.1
prunus_persica Prupe.1G432100_v2.0.a1
pyrus_communis pycom08g07830 pycom15g07360
rosa_chinensis RchiOBHm_Chr6g0308351
rosa_laevigata RLG00000010648
rosa_multiflora Rmu_sc0005523.1_g000011
rosa_roxburghii Rroxscaffold_7G00160220
rosa_rugosa Rorug06G0366200
rosa_samantha Rh6AG475500 Rh6BG485900 Rh6CG491100 Rh6DG477400
rosa_wichuraiana Rw6G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 277, 444
AciI CCGC 1 cut(s) 337
AcvI CACGTG 1 cut(s) 38
AfaI GTAC 1 cut(s) 389
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 188, 250
AflIII ACRYGT 2 cut(s) 275, 603
AgsI TTSAA 2 cut(s) 71, 401
AjnI CCWGG 1 cut(s) 249
AloI GAACNNNNNNTCC 2 cut(s) 487, 519
AluBI AGCT 4 cut(s) 171, 205, 379, 552
AluI AGCT 4 cut(s) 171, 205, 379, 552
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 265
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 12, 465
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 2 cut(s) 99, 542
AspS9I GGNCC 1 cut(s) 191
AsuC2I CCSGG 2 cut(s) 266, 267
AsuHPI GGTGA 1 cut(s) 472
AsuNHI GCTAGC 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 265
BbrPI CACGTG 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 532
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 24, 477
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 2 cut(s) 266, 267
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 2 cut(s) 195, 206
BfmI CTRYAG 1 cut(s) 535
BfoI RGCGCY 1 cut(s) 100
BisI GCNGC 2 cut(s) 13, 466
BlpI GCTNAGC 2 cut(s) 351, 375
BlsI GCNGC 2 cut(s) 14, 467
Bme1390I CCNGG 3 cut(s) 251, 266, 267
BmeT110I CYCGRG 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 425
BmrFI CCNGG 3 cut(s) 251, 266, 267
BmrI ACTGGG 1 cut(s) 605
BmsI GCATC 4 cut(s) 34, 80, 218, 249
BmtI GCTAGC 1 cut(s) 209
BmuI ACTGGG 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 80
Bpu1102I GCTNAGC 2 cut(s) 351, 375
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 2 cut(s) 266, 267
Bsa29I ATCGAT 1 cut(s) 580
BsaAI YACGTR 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 3 cut(s) 85, 249, 265
BsaXI ACNNNNNCTCC 6 cut(s) 136, 166, 472, 487, 502, 517
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 250
Bse1I ACTGG 2 cut(s) 425, 600
BseBI CCWGG 1 cut(s) 251
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 85, 249, 265
BseGI GGATG 2 cut(s) 53, 95
BseLI CCNNNNNNNGG 2 cut(s) 188, 250
BseMII CTCAG 3 cut(s) 94, 365, 389
BseNI ACTGG 2 cut(s) 425, 600
BseRI GAGGAG 2 cut(s) 204, 342
BseXI GCAGC 2 cut(s) 24, 477
Bsh1236I CGCG 2 cut(s) 277, 444
Bsh1285I CGRYCG 1 cut(s) 584
BshFI GGCC 1 cut(s) 192
BshVI ATCGAT 1 cut(s) 580
BsiEI CGRYCG 1 cut(s) 584
BsiHKAI GWGCWC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 2 cut(s) 62, 286
BslI CCNNNNNNNGG 2 cut(s) 188, 250
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 2 cut(s) 62, 286
BsmI GAATGC 2 cut(s) 339, 352
BsnI GGCC 1 cut(s) 192
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 532
Bsp143I GATC 3 cut(s) 160, 577, 581
Bsp1720I GCTNAGC 2 cut(s) 351, 375
BspACI CCGC 1 cut(s) 337
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 3 cut(s) 93, 364, 388
BspDI ATCGAT 1 cut(s) 580
BspFNI CGCG 2 cut(s) 277, 444
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 425
BspOI GCTAGC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 482
BsrI ACTGG 2 cut(s) 425, 600
BssECI CCNNGG 3 cut(s) 85, 249, 265
BssMI GATC 3 cut(s) 160, 577, 581
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 523
BstBAI YACGTR 1 cut(s) 38
BstC8I GCNNGC 3 cut(s) 95, 207, 442
BstDEI CTNAG 3 cut(s) 80, 351, 375
BstF5I GGATG 2 cut(s) 53, 95
BstFNI CGCG 2 cut(s) 277, 444
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 2 cut(s) 99, 542
BstKTI GATC 3 cut(s) 163, 580, 584
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 3 cut(s) 160, 577, 581
BstMCI CGRYCG 1 cut(s) 584
BstMWI GCNNNNNNNGC 3 cut(s) 90, 361, 390
BstNI CCWGG 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 607
BstSCI CCNGG 3 cut(s) 249, 264, 265
BstSFI CTRYAG 1 cut(s) 535
BstUI CGCG 2 cut(s) 277, 444
BstV1I GCAGC 2 cut(s) 24, 477
Bsu15I ATCGAT 1 cut(s) 580
BsuRI GGCC 1 cut(s) 192
BsuTUI ATCGAT 1 cut(s) 580
BtsCI GGATG 2 cut(s) 53, 95
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 95, 207, 442
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 99, 542
Cfr13I GGNCC 1 cut(s) 191
Cfr9I CCCGGG 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 580
CseI GACGC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 388
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 5 cut(s) 16, 218, 224, 555, 604
CviJI RGCY 6 cut(s) 84, 171, 192, 205, 379, 552
CviKI_1 RGCY 6 cut(s) 84, 171, 192, 205, 379, 552
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 80, 351, 375
DpnI GATC 3 cut(s) 162, 579, 583
DpnII GATC 3 cut(s) 160, 577, 581
Eco130I CCWWGG 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 482
Eco47III AGCGCT 1 cut(s) 98
Eco72I CACGTG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 5 cut(s) 19, 221, 227, 558, 607
FaiI YATR 6 cut(s) 17, 219, 225, 324, 556, 605
FaqI GGGAC 2 cut(s) 62, 286
FatI CATG 5 cut(s) 15, 217, 223, 554, 603
Fnu4HI GCNGC 2 cut(s) 13, 466
FokI GGATG 2 cut(s) 40, 102
Fsp4HI GCNGC 2 cut(s) 13, 466
FspBI CTAG 2 cut(s) 195, 206
GlaI GCGC 2 cut(s) 98, 541
GluI GCNGC 2 cut(s) 13, 466
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 1 cut(s) 192
HapII CCGG 1 cut(s) 266
HgaI GACGC 1 cut(s) 283
HhaI GCGC 2 cut(s) 99, 542
Hin1II CATG 5 cut(s) 19, 221, 227, 558, 607
Hin6I GCGC 2 cut(s) 97, 540
HinP1I GCGC 2 cut(s) 97, 540
HinfI GANTC 5 cut(s) 42, 50, 236, 283, 340
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 3 cut(s) 127, 179, 345
Hpy188III TCNNGA 3 cut(s) 68, 166, 218
Hpy8I GTNNAC 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 13, 395
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 4 cut(s) 25, 93, 137, 262
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 361, 390
HpyF3I CTNAG 3 cut(s) 80, 351, 375
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 5 cut(s) 19, 221, 227, 558, 607
HspAI GCGC 2 cut(s) 97, 540
Kzo9I GATC 3 cut(s) 160, 577, 581
LmnI GCTCC 3 cut(s) 118, 157, 535
LpnPI CCDG 7 cut(s) 31, 236, 263, 279, 406, 519, 581
Lsp1109I GCAGC 2 cut(s) 24, 477
LweI GCATC 4 cut(s) 34, 80, 218, 249
MaeI CTAG 2 cut(s) 195, 206
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 599
MalI GATC 3 cut(s) 162, 579, 583
MboI GATC 3 cut(s) 160, 577, 581
MboII GAAGA 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 2 cut(s) 510, 546
MluI ACGCGT 1 cut(s) 275
MlyI GAGTC 3 cut(s) 36, 44, 334
MmeI TCCRAC 3 cut(s) 150, 260, 311
MnlI CCTC 9 cut(s) 85, 89, 117, 225, 250, 256, 263, 320, 323
MroXI GAANNNNTTC 1 cut(s) 413
MslI CAYNNNNRTG 2 cut(s) 20, 222
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 3 cut(s) 251, 266, 267
Mva1269I GAATGC 2 cut(s) 339, 352
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 2 cut(s) 277, 444
MwoI GCNNNNNNNGC 3 cut(s) 90, 361, 390
NciI CCSGG 2 cut(s) 266, 267
NdeII GATC 3 cut(s) 160, 577, 581
NheI GCTAGC 1 cut(s) 205
NlaIII CATG 5 cut(s) 19, 221, 227, 558, 607
NlaIV GGNNCC 1 cut(s) 425
NspI RCATGY 1 cut(s) 607
PagI TCATGA 1 cut(s) 217
PciI ACATGT 1 cut(s) 603
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 2 cut(s) 339, 352
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 236, 283
PkrI GCNGC 2 cut(s) 14, 467
Ple19I CGATCG 1 cut(s) 584
PleI GAGTC 3 cut(s) 36, 44, 334
PmaCI CACGTG 1 cut(s) 38
PmlI CACGTG 1 cut(s) 38
PpsI GAGTC 3 cut(s) 36, 44, 334
Ppu21I YACGTR 1 cut(s) 38
PscI ACATGT 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 249
PspCI CACGTG 1 cut(s) 38
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 425
PspPI GGNCC 1 cut(s) 191
PvuI CGATCG 1 cut(s) 584
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 2 cut(s) 20, 222
SatI GCNGC 2 cut(s) 13, 466
Sau3AI GATC 3 cut(s) 160, 577, 581
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 3 cut(s) 36, 44, 334
ScrFI CCNGG 3 cut(s) 251, 266, 267
SduI GDGCHC 1 cut(s) 532
SetI ASST 7 cut(s) 24, 40, 173, 207, 255, 381, 554
SfaNI GCATC 4 cut(s) 34, 80, 218, 249
SfcI CTRYAG 1 cut(s) 535
SmaI CCCGGG 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 20, 222
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 510, 546
SsiI CCGC 1 cut(s) 337
SspMI CTAG 2 cut(s) 195, 206
StyD4I CCNGG 3 cut(s) 249, 264, 265
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 523
TaiI ACGT 1 cut(s) 40
TaqI TCGA 2 cut(s) 159, 580
TasI AATT 2 cut(s) 510, 546
TfiI GAWTC 2 cut(s) 236, 283
TscAI CASTG 1 cut(s) 376
TseI GCWGC 2 cut(s) 12, 465
TspDTI ATGAA 2 cut(s) 32, 163
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 1 cut(s) 376
XceI RCATGY 1 cut(s) 607
XmaI CCCGGG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 2 cut(s) 195, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.