Rh6CG491100

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012231
Physical Location & Seq
Reverse (-)
65814177 .. 65816908
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG491100.1

Sequence Viewer

Length: 444 bp
ATGCAGGTAGAGATGCACCCGGGAGGGACGCGTTGGAATCCAACACAAGAGCAGATAGGGATATTGGAAATGCTGTATAGAGGAGGAATGCGGACTCCGAATGCTCAGCAGATAGAGCAAATCACTGCTCAGCTTGGCAAGTACGGCAAGATTGAAGGCAAGAATGTGTTCTACTGGTTCCAAAATCACAAAGCACGCGAAAGACAGAAGCAGAAACGCAGCACTCTTGGTCTCACGCACTCTCCAAGAACCCCGACTCCTGTAATTACCACCACCGGGGAAGTACTGGAAAGAGAGGAAGACAGTCCATACAAGAGAAAGTGCAGGAGCTGGGCATTTGATTGTTTGGGAGACGATTCTAGTATTATAACTTGTAACAAAGACGTCGTTGAGGGAGATAGAACTCTGGAGCTCTTCCCATTACGCCCAGAAGGCATGAGATGA

Protein Analysis

147

Amino Acids

16.97

Weight (kDa)

8.82

Isoelectric Point (pI)

60.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 10 - 69 4.2e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46480 AT1G46480
fragaria_vesca FvH4_2g34030 FvH4_2g34030
malus_domestica MD08G1096300.v1.1 MD15G1079000.v1.1
prunus_persica Prupe.1G432100_v2.0.a1
pyrus_communis pycom08g07830 pycom15g07360
rosa_chinensis RchiOBHm_Chr6g0308351
rosa_laevigata RLG00000010648
rosa_multiflora Rmu_sc0005523.1_g000011
rosa_roxburghii Rroxscaffold_7G00160220
rosa_rugosa Rorug06G0366200
rosa_samantha Rh6AG475500 Rh6BG485900 Rh6CG491100 Rh6DG477400
rosa_wichuraiana Rw6G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AatII GACGTC 1 cut(s) 387
AccII CGCG 2 cut(s) 31, 198
AciI CCGC 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 2 cut(s) 143, 285
AfiI CCNNNNNNNGG 1 cut(s) 276
AflIII ACRYGT 1 cut(s) 29
AgsI TTSAA 1 cut(s) 155
AloI GAACNNNNNNTCC 2 cut(s) 241, 273
AluBI AGCT 3 cut(s) 133, 330, 412
AluI AGCT 3 cut(s) 133, 330, 412
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 236, 345
AlwNI CAGNNNCTG 1 cut(s) 330
Ama87I CYCGRG 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 219
Asp700I GAANNNNTTC 1 cut(s) 167
AsuC2I CCSGG 3 cut(s) 20, 21, 277
AvaI CYCGRG 1 cut(s) 19
BanII GRGCYC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 1 cut(s) 231
BceAI ACGGC 1 cut(s) 160
BcnI CCSGG 3 cut(s) 20, 21, 277
BcoDI GTCTC 2 cut(s) 236, 345
BfaI CTAG 1 cut(s) 360
BglI GCCNNNNNGGC 1 cut(s) 432
BisI GCNGC 1 cut(s) 220
BlpI GCTNAGC 2 cut(s) 105, 129
BlsI GCNGC 1 cut(s) 221
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 3 cut(s) 20, 21, 277
BmeT110I CYCGRG 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 179
BmrFI CCNGG 3 cut(s) 20, 21, 277
BmsI GCATC 1 cut(s) 3
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 428
Bpu1102I GCTNAGC 2 cut(s) 105, 129
BpuMI CCSGG 3 cut(s) 20, 21, 277
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 19, 276
BsaXI ACNNNNNCTCC 4 cut(s) 226, 241, 256, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse1I ACTGG 2 cut(s) 179, 291
BseDI CCNNGG 2 cut(s) 19, 276
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 2 cut(s) 119, 143
BseNI ACTGG 2 cut(s) 179, 291
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 231
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 31, 198
BsiHKAI GWGCWC 1 cut(s) 414
BsiHKCI CYCGRG 1 cut(s) 19
BsiSI CCGG 2 cut(s) 20, 276
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 236, 345
BsmBI CGTCTC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 2 cut(s) 93, 106
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 414
Bsp1720I GCTNAGC 2 cut(s) 105, 129
BspACI CCGC 1 cut(s) 91
BspCNI CTCAG 2 cut(s) 118, 142
BspFNI CGCG 2 cut(s) 31, 198
BspLI GGNNCC 1 cut(s) 179
BspQI GCTCTTC 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 236
BsrI ACTGG 2 cut(s) 179, 291
BssECI CCNNGG 2 cut(s) 19, 276
BssNI GRCGYC 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 2 cut(s) 105, 129
BstFNI CGCG 2 cut(s) 31, 198
BstMAI GTCTC 2 cut(s) 236, 345
BstMWI GCNNNNNNNGC 3 cut(s) 115, 144, 432
BstSCI CCNGG 3 cut(s) 18, 19, 275
BstUI CGCG 2 cut(s) 31, 198
BstV1I GCAGC 1 cut(s) 231
BstV2I GAAGAC 1 cut(s) 306
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 330
Cfr9I CCCGGG 1 cut(s) 19
CseI GACGC 1 cut(s) 37
Csp6I GTAC 2 cut(s) 142, 284
CviAII CATG 1 cut(s) 436
CviJI RGCY 3 cut(s) 133, 330, 412
CviKI_1 RGCY 3 cut(s) 133, 330, 412
CviQI GTAC 2 cut(s) 142, 284
DdeI CTNAG 2 cut(s) 105, 129
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 412
Eco88I CYCGRG 1 cut(s) 19
EcoICRI GAGCTC 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 345
FaeI CATG 1 cut(s) 439
FaiI YATR 4 cut(s) 78, 310, 368, 437
FaqI GGGAC 1 cut(s) 40
FatI CATG 1 cut(s) 435
Fnu4HI GCNGC 1 cut(s) 220
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 220
FspBI CTAG 1 cut(s) 360
GluI GCNGC 1 cut(s) 220
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 428
HapII CCGG 2 cut(s) 20, 276
HgaI GACGC 1 cut(s) 37
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 1 cut(s) 439
HinfI GANTC 4 cut(s) 37, 94, 256, 356
HpaII CCGG 2 cut(s) 20, 276
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 1 cut(s) 407
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 149, 425
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 3 cut(s) 4, 16, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 144, 432
HpyF3I CTNAG 2 cut(s) 105, 129
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 1 cut(s) 439
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 2 cut(s) 327, 409
LpnPI CCDG 8 cut(s) 33, 160, 272, 273, 289, 310, 316, 392
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 1 cut(s) 3
MaeI CTAG 1 cut(s) 360
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 374
MboII GAAGA 2 cut(s) 311, 406
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 1 cut(s) 264
MluI ACGCGT 1 cut(s) 29
MlyI GAGTC 2 cut(s) 88, 250
MmeI TCCRAC 2 cut(s) 14, 65
MnlI CCTC 5 cut(s) 17, 74, 77, 289, 385
MroXI GAANNNNTTC 1 cut(s) 167
MspI CCGG 2 cut(s) 20, 276
MspR9I CCNGG 3 cut(s) 20, 21, 277
Mva1269I GAATGC 2 cut(s) 93, 106
MvnI CGCG 2 cut(s) 31, 198
MwoI GCNNNNNNNGC 3 cut(s) 115, 144, 432
NciI CCSGG 3 cut(s) 20, 21, 277
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 419
PctI GAATGC 2 cut(s) 93, 106
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 37, 356
PkrI GCNGC 1 cut(s) 221
PleI GAGTC 2 cut(s) 88, 250
PpsI GAGTC 2 cut(s) 88, 250
PsiI TTATAA 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 414
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 330
RsaI GTAC 2 cut(s) 143, 285
RsaNI GTAC 2 cut(s) 142, 284
SacI GAGCTC 1 cut(s) 414
SapI GCTCTTC 1 cut(s) 419
SatI GCNGC 1 cut(s) 220
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 2 cut(s) 88, 250
ScrFI CCNGG 3 cut(s) 20, 21, 277
SduI GDGCHC 1 cut(s) 414
SetI ASST 5 cut(s) 9, 135, 332, 387, 414
SfaNI GCATC 1 cut(s) 3
SmaI CCCGGG 1 cut(s) 21
Sse9I AATT 1 cut(s) 264
SsiI CCGC 1 cut(s) 91
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 18, 19, 275
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 387
TasI AATT 1 cut(s) 264
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 2 cut(s) 37, 356
TscAI CASTG 1 cut(s) 130
TseI GCWGC 1 cut(s) 219
TspMI CCCGGG 1 cut(s) 19
TspRI CASTG 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 1 cut(s) 360
ZraI GACGTC 1 cut(s) 385
ZrmI AGTACT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.