MD08G1102700.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009593
Physical Location & Seq
Reverse (-)
8716912 .. 8722210
5299 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1102700.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGTCCACCAAGAGCGAAAATCATCAGACGGTTCCGCTTTCGGTGCTGCTCAAGCGTGAATTGGCCAATGAGAAGATTGAGAGACCGGAGCTTATACACGGTCAGGCGAGTCAGAGCAAGAAAGGAGAGGATTTCACGTTGATAAAGACGGAATGTCAAAGAATTGTGGGAGATGGAGTTTCTACGTATTCAGTTTTCGGGCTATTTGATGGGCATAACGGGTCAGCAGCCGCTATCTACTCTAAGGAGAATCTTCTGAATAATGTTTTGGCCGCTATCCCTTCAGATCTAAACAGAGATGAATGGGTAGCAGCATTGCCAAGGGCTTTTGTAGCAGGCTTCGTCAAGACAGACAAAGAATTCCAAGAGAGAGCTAAAACGTCAGGAACAACTGTCACATTTGTGATTATAGAAGGATGGGTCATATCCGTTGCATCTGTTGGCGATTCTCGTTGCATAGTTGAACCTGCTGAAGGTGGAGTTTATTATTTATCAGCAGATCATAGGCTTGAAATCAATGAAGAGGAGAGGCAGCGTATCACCTCAAGTGGGGGCGAGGTTGGTCGGCTTAATACTGGTGGTGGAGCAGAGATTGGTCCTTTGAGGTGTTGGCCTGGAGGCTTGTGTCTGTCACGATCCATTGGTGATCTGGATGTGGGGGAGTTCATTGTTCCTGTTCCTTATGTGAAGCAAATCAAGTTGTCCAGCGCAGGTGGTAGAATTATCATATCAAGTGACGGAGTCTGGGATGCTTTGTCTGCAGAAGAAGCTCTTGATTGTTGCCGTGGGATGCCACCGGACACGGCAGCTGCACAAATTGTTAAGGACGCTGTAGGGCATAAGGGACTTCGGGATGATACAACCTGCATTGTGATTGATATTTTACCACAAGAGAAGCCACAAGCTCCGTTATCCCAACCAAAGAGGCACGGAAAAGGAGTGTTGTTTAAATCCATGTTTAAGAAAAAGTCCTCTGAATCATCTTCTAATGTTGAAAAAGAATATATTGAGCCAGACGAGGTGGAGGAATTATTTGAGGAAGGTTCTGCTTCACTTTCCGAAAGGTTAGATACAAAATACCCGCTCTGCAACATGTTCAAGTTGTTTATGTGTGCAGTTTGTCAAGTAGAAATTAAACCGGGAGAGGGTATTTCAATACACACTGGGTCATCGAACCCAGGAAAAGTGCGACCTTGGGATGGCCCCTTCCTTTGCTCGAGTTGCCAAGAGAAAAAAGAGGCTATGGAGGGGCAAAGACCGTCAGGAAGAAGACGCGATAGCGATAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

431

Amino Acids

46.81

Weight (kDa)

5.3

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 54 - 277 3.6e-30 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 701
Acc36I ACCTGC 3 cut(s) 475, 701, 872
AccBSI CCGCTC 1 cut(s) 1084
AccII CGCG 1 cut(s) 1275
AciI CCGC 4 cut(s) 35, 231, 273, 1082
AclWI GGATC 1 cut(s) 630
AcoI YGGCCR 2 cut(s) 63, 270
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 267, 492
AfiI CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 5 cut(s) 464, 512, 995, 1099, 1155
AhdI GACNNNNNGTC 1 cut(s) 153
AjnI CCWGG 2 cut(s) 613, 1177
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 5 cut(s) 91, 374, 770, 809, 905
AluI AGCT 5 cut(s) 91, 374, 770, 809, 905
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 1216
AoxI GGCC 4 cut(s) 63, 270, 611, 1201
ApeKI GCWGC 6 cut(s) 46, 227, 311, 532, 806, 809
ApoI RAATTY 1 cut(s) 359
AspLEI GCGC 1 cut(s) 710
AspS9I GGNCC 2 cut(s) 596, 1202
AsuC2I CCSGG 1 cut(s) 1140
AsuHPI GGTGA 2 cut(s) 532, 656
AvaI CYCGRG 1 cut(s) 1216
AvaII GGWCC 1 cut(s) 596
BalI TGGCCA 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 1276
BbvI GCAGC 6 cut(s) 33, 239, 323, 544, 796, 818
BccI CCATC 4 cut(s) 167, 203, 411, 1193
BceAI ACGGC 2 cut(s) 768, 819
BciT130I CCWGG 2 cut(s) 615, 1179
BcnI CCSGG 1 cut(s) 1140
BcoDI GTCTC 1 cut(s) 76
BfmI CTRYAG 2 cut(s) 759, 831
BfuAI ACCTGC 3 cut(s) 475, 701, 872
BglII AGATCT 1 cut(s) 286
BisI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 533, 807, 810
BlsI GCNGC 8 cut(s) 48, 229, 232, 274, 313, 534, 808, 811
Bme1390I CCNGG 3 cut(s) 615, 1140, 1179
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 1216
BmgT120I GGNCC 2 cut(s) 596, 1202
BmiI GGNNCC 2 cut(s) 33, 1204
BmrFI CCNGG 3 cut(s) 615, 1140, 1179
BmrI ACTGGG 1 cut(s) 1173
BmsI GCATC 3 cut(s) 443, 739, 780
BmuI ACTGGG 1 cut(s) 1173
BpiI GAAGAC 1 cut(s) 1276
BpmI CTGGAG 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 35, 529
BpuMI CCSGG 1 cut(s) 1140
BsaAI YACGTR 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 320, 784, 1177, 1193
BsaWI WCCGGW 2 cut(s) 85, 796
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
Bse1I ACTGG 2 cut(s) 580, 1168
Bse3DI GCAATG 1 cut(s) 314
BseBI CCWGG 2 cut(s) 615, 1179
BseDI CCNNGG 4 cut(s) 320, 784, 1177, 1193
BseGI GGATG 6 cut(s) 422, 658, 754, 795, 859, 1204
BseLI CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
BseMI GCAATG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 580, 1168
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 6 cut(s) 33, 239, 323, 544, 796, 818
BsgI GTGCAG 2 cut(s) 795, 1134
Bsh1236I CGCG 1 cut(s) 1275
BshFI GGCC 4 cut(s) 65, 272, 613, 1203
BsiHKCI CYCGRG 1 cut(s) 1216
BsiSI CCGG 3 cut(s) 86, 797, 1139
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 858
BsnI GGCC 4 cut(s) 65, 272, 613, 1203
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 1216
Bsp143I GATC 4 cut(s) 286, 499, 635, 646
BspACI CCGC 4 cut(s) 35, 231, 273, 1082
BspANI GGCC 4 cut(s) 65, 272, 613, 1203
BspFNI CGCG 1 cut(s) 1275
BspLI GGNNCC 2 cut(s) 33, 1204
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 3 cut(s) 475, 701, 872
BspPI GGATC 1 cut(s) 630
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 1084
BsrDI GCAATG 1 cut(s) 314
BsrI ACTGG 2 cut(s) 580, 1168
BssECI CCNNGG 4 cut(s) 320, 784, 1177, 1193
BssMI GATC 4 cut(s) 286, 499, 635, 646
BssT1I CCWWGG 2 cut(s) 320, 1193
Bst2UI CCWGG 2 cut(s) 615, 1179
Bst4CI ACNGT 4 cut(s) 31, 101, 394, 1260
Bst6I CTCTTC 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 243
BstDSI CCRYGG 1 cut(s) 784
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 6 cut(s) 422, 658, 754, 795, 859, 1204
BstFNI CGCG 1 cut(s) 1275
BstHHI GCGC 1 cut(s) 710
BstKTI GATC 4 cut(s) 289, 502, 638, 649
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 4 cut(s) 286, 499, 635, 646
BstMWI GCNNNNNNNGC 6 cut(s) 43, 52, 332, 758, 767, 1221
BstNI CCWGG 2 cut(s) 615, 1179
BstNSI RCATGY 1 cut(s) 1096
BstSCI CCNGG 3 cut(s) 613, 1138, 1177
BstSFI CTRYAG 2 cut(s) 759, 831
BstSNI TACGTA 1 cut(s) 186
BstUI CGCG 1 cut(s) 1275
BstV1I GCAGC 6 cut(s) 33, 239, 323, 544, 796, 818
BstV2I GAAGAC 1 cut(s) 1276
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuRI GGCC 4 cut(s) 65, 272, 613, 1203
BtgI CCRYGG 1 cut(s) 784
BtsCI GGATG 6 cut(s) 422, 658, 754, 795, 859, 1204
BtsIMutI CAGTG 1 cut(s) 1161
BveI ACCTGC 3 cut(s) 475, 701, 872
Cac8I GCNNGC 1 cut(s) 337
CfoI GCGC 1 cut(s) 710
Cfr13I GGNCC 2 cut(s) 596, 1202
CseI GACGC 2 cut(s) 836, 1281
CviAII CATG 2 cut(s) 955, 1093
DdeI CTNAG 1 cut(s) 243
DpnI GATC 4 cut(s) 288, 501, 637, 648
DpnII GATC 4 cut(s) 286, 499, 635, 646
DraI TTTAAA 1 cut(s) 949
DriI GACNNNNNGTC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 63, 270
Eam1104I CTCTTC 1 cut(s) 516
Eam1105I GACNNNNNGTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 516
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 320, 1193
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 267, 492
Eco88I CYCGRG 1 cut(s) 1216
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 613, 1177
EcoT14I CCWWGG 2 cut(s) 320, 1193
ErhI CCWWGG 2 cut(s) 320, 1193
FaeI CATG 2 cut(s) 958, 1096
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 858
FatI CATG 2 cut(s) 954, 1092
FauI CCCGC 1 cut(s) 1089
Fnu4HI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 533, 807, 810
FokI GGATG 6 cut(s) 429, 665, 761, 802, 866, 1211
Fsp4HI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 533, 807, 810
GlaI GCGC 1 cut(s) 709
GluI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 533, 807, 810
GsuI CTGGAG 1 cut(s) 636
HaeIII GGCC 4 cut(s) 65, 272, 613, 1203
HapII CCGG 3 cut(s) 86, 797, 1139
HgaI GACGC 2 cut(s) 836, 1281
HhaI GCGC 1 cut(s) 710
Hin1II CATG 2 cut(s) 958, 1096
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HinfI GANTC 5 cut(s) 109, 250, 446, 741, 977
HpaII CCGG 3 cut(s) 86, 797, 1139
HphI GGTGA 2 cut(s) 532, 656
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 6 cut(s) 27, 114, 258, 286, 976, 1060
Hpy188III TCNNGA 7 cut(s) 346, 384, 633, 650, 773, 851, 1263
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 5 cut(s) 291, 407, 467, 1034, 1216
HpyCH4III ACNGT 4 cut(s) 31, 101, 394, 1260
HpyCH4IV ACGT 3 cut(s) 137, 185, 380
HpyCH4V TGCA 7 cut(s) 434, 456, 761, 812, 867, 1089, 1115
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 52, 332, 758, 767, 1221
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 3 cut(s) 137, 185, 380
Hsp92II CATG 2 cut(s) 958, 1096
HspAI GCGC 1 cut(s) 708
Kzo9I GATC 4 cut(s) 286, 499, 635, 646
LmnI GCTCC 3 cut(s) 88, 584, 910
Lsp1109I GCAGC 6 cut(s) 33, 239, 323, 544, 796, 818
LweI GCATC 3 cut(s) 443, 739, 780
MaeII ACGT 3 cut(s) 137, 185, 380
MaeIII GTNAC 3 cut(s) 394, 630, 734
MalI GATC 4 cut(s) 288, 501, 637, 648
MbiI CCGCTC 1 cut(s) 1084
MboI GATC 4 cut(s) 286, 499, 635, 646
MboII GAAGA 7 cut(s) 85, 245, 533, 776, 975, 1278, 1281
MflI RGATCY 1 cut(s) 286
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 7 cut(s) 59, 162, 359, 720, 816, 1028, 1131
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 2 cut(s) 118, 750
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 5 cut(s) 570, 822, 948, 960, 1134
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 3 cut(s) 86, 797, 1139
MspR9I CCNGG 3 cut(s) 615, 1140, 1179
MvaI CCWGG 2 cut(s) 615, 1179
MvnI CGCG 1 cut(s) 1275
MwoI GCNNNNNNNGC 6 cut(s) 43, 52, 332, 758, 767, 1221
NciI CCSGG 1 cut(s) 1140
NdeII GATC 4 cut(s) 286, 499, 635, 646
NlaIII CATG 2 cut(s) 958, 1096
NlaIV GGNNCC 2 cut(s) 33, 1204
NmuCI GTSAC 3 cut(s) 394, 630, 734
NspI RCATGY 1 cut(s) 1096
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 1216
PaqCI CACCTGC 1 cut(s) 701
PciI ACATGT 1 cut(s) 1092
PfeI GAWTC 3 cut(s) 250, 446, 977
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 8 cut(s) 48, 229, 232, 274, 313, 534, 808, 811
PleI GAGTC 2 cut(s) 117, 749
PpsI GAGTC 2 cut(s) 117, 749
Ppu21I YACGTR 1 cut(s) 186
PscI ACATGT 1 cut(s) 1092
Psp6I CCWGG 2 cut(s) 613, 1177
PspGI CCWGG 2 cut(s) 613, 1177
PspN4I GGNNCC 2 cut(s) 33, 1204
PspPI GGNCC 2 cut(s) 596, 1202
PspXI VCTCGAGB 1 cut(s) 1216
PstI CTGCAG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 286
PsyI GACNNNGTC 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 809
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 5 cut(s) 570, 822, 948, 960, 1134
SatI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 533, 807, 810
Sau3AI GATC 4 cut(s) 286, 499, 635, 646
Sau96I GGNCC 2 cut(s) 596, 1202
SchI GAGTC 2 cut(s) 118, 750
ScrFI CCNGG 3 cut(s) 615, 1140, 1179
SfaNI GCATC 3 cut(s) 443, 739, 780
SfcI CTRYAG 2 cut(s) 759, 831
Sfr274I CTCGAG 1 cut(s) 1216
SinI GGWCC 1 cut(s) 596
SlaI CTCGAG 1 cut(s) 1216
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 3 cut(s) 50, 544, 1216
SmoI CTYRAG 3 cut(s) 50, 544, 1216
SnaBI TACGTA 1 cut(s) 186
Sse9I AATT 7 cut(s) 59, 162, 359, 720, 816, 1028, 1131
SsiI CCGC 4 cut(s) 35, 231, 273, 1082
StyD4I CCNGG 3 cut(s) 613, 1138, 1177
StyI CCWWGG 2 cut(s) 320, 1193
TaaI ACNGT 4 cut(s) 31, 101, 394, 1260
TaiI ACGT 3 cut(s) 140, 188, 383
TaqI TCGA 2 cut(s) 1172, 1217
TasI AATT 7 cut(s) 59, 162, 359, 720, 816, 1028, 1131
TauI GCSGC 2 cut(s) 233, 275
TfiI GAWTC 3 cut(s) 250, 446, 977
Tru1I TTAA 5 cut(s) 570, 822, 948, 960, 1134
Tru9I TTAA 5 cut(s) 570, 822, 948, 960, 1134
TscAI CASTG 1 cut(s) 1168
TseFI GTSAC 3 cut(s) 394, 630, 734
TseI GCWGC 6 cut(s) 46, 227, 311, 532, 806, 809
Tsp45I GTSAC 3 cut(s) 394, 630, 734
TspDTI ATGAA 3 cut(s) 315, 534, 655
TspGWI ACGGA 5 cut(s) 164, 418, 753, 897, 945
TspRI CASTG 1 cut(s) 1168
Tth111I GACNNNGTC 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.