RLG00000010703

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009593
Physical Location & Seq
Reverse (-)
3747309 .. 3753410
6102 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010703

Sequence Viewer

Length: 1557 bp
ATGCTTCTACGTCTGGCTTTTCGCCCAGGAACACGTTTGCATGAGCTTCGATCCTCTGCGGCGCTGAGTATCATAAGCCAAGCGCCGGGATCCGCGTTTCTCGATCGGACCAATCAACCGTCGTATTCGCCGTGCCGCTTCGCGCCTAAACCGCCATTGCTCTTCCGCTATCTTTCTCTCTCTATCTGTAACAGATTTGGTTTGAGAATCGGGCATGGAGGATTAGAGTTGGGAGGAGAGGCTGTGTTATTAGGGGTGAGAACAATGTCCAACAGGAGTGATAATCATCAGACGGTCCCGCTTTCGGTGTTGCTAAAGCGAGAATTGGCCAATGAGAAGATTGAGAGGCCCGAGATTATATACGGTCAGGCCAGTCAGAGCAAGAAAGGGGAGGATTTCACTATGGTAAAGACGGATTGTCAAAGAGTTGTGGGAGATGGAGTTTCTACCTATTCAGTTTTCGCGCTATTTGATGGGCATAACGGGTCTGCAGCCGCTTTATACTCCAAGGAAGCTCTTCTAAACAATATTTTAGCTGCTATCCCCACAGATCTTAACAGAGATGAATGGATTGCAGCTTTGCCAAGGTCGTTGGTAGCAGGCTTTGTCAAGACAGACAAAGATTTCCAAGAGAGAGCTCAAACGTCAGGAACCACTGTCACCTTTGTAATTATAGAGGGATGGGTAATATCTGTTGCATCGGTTGGCGATTCTCGTTGCATAGTTGAACCTGCTGAAGGTGGAGTTTATTATTTATCAGCAGATCATAGGCTTGAATGCAGCGAAGACGAGAGGATGCGTATCACTTCAAGTGGCGGTGAGGTTGGTCGGCTGAATACTGGCGGTGGGGCAGAGATTGGTCCCTTGAGGTGTTGGCCTGGTGGTTTGTGTCTTTCAAGATCCATTGGGGATATGGACGTAGGCGAGTTCATTGTTCCTGTTCCTTATGTAAAGCAAATGAAGTTGTCTACTGCAGGTGGTAGGGTAATCATATCAAGTGATGGTGTATGGGATGCTCTGTCCGCAGAAGAAGCTCTTGACTGTTGTCGTGGGATGCCACCGGATGCTGCAGCTGCCCAAGTAGTCAAGGATGCTGTTGGGGCAAAGGGACTTCGGGATGATACAACCTGTATTGTGATTGACATTTTACCACAGGAGAAGCCTGCGGCTCCATTGCCACCACCAAAGAGGCAAGGAAAAGGAGTTTTTAAGTCCATGTTTAAGAAAAAGCCTAATGAATCATCTTCTACTGTTGAAAAAGAATATCTTGAGCCAGACACGGTGGAGGAAATATTTGAGGAAGGTTCTGCTTCTCTTTCCGAAAGGTTAGATTCAAAGTACCCACTCTGCAACATGTTTAAGCTCTTTATGTGTGCTGTTTGTCAAGTTGAAATCAAACCAGGGGAGGGTATTTCAATACACTCCGGGTCATCCTCTAACCCAGAAAAAGTGCGACCTTGGGATGGCCCCTTCCTTTGTACTAGTTGCCAAGAGAAGAAAGAGGCTATGGAAGGGAAAAGACCATCAGGAAATCGGCGTGACAGCGATAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

519

Amino Acids

56.19

Weight (kDa)

5.77

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 146 - 365 2.5e-28 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 965
Acc36I ACCTGC 2 cut(s) 739, 965
AccI GTMKAC 1 cut(s) 968
AccII CGCG 3 cut(s) 95, 143, 464
AclWI GGATC 4 cut(s) 45, 84, 97, 894
AcoI YGGCCR 1 cut(s) 327
AcuI CTGAAG 1 cut(s) 756
AfaI GTAC 2 cut(s) 1340, 1480
AfiI CCNNNNNNNGG 5 cut(s) 85, 304, 737, 1406, 1463
AflIII ACRYGT 2 cut(s) 32, 1353
AgsI TTSAA 8 cut(s) 728, 776, 810, 897, 1256, 1335, 1391, 1416
AhdI GACNNNNNGTC 1 cut(s) 417
AhlI ACTAGT 1 cut(s) 1481
AjnI CCWGG 3 cut(s) 25, 877, 1399
AluBI AGCT 8 cut(s) 46, 515, 536, 578, 638, 1034, 1073, 1363
AluI AGCT 8 cut(s) 46, 515, 536, 578, 638, 1034, 1073, 1363
Alw21I GWGCWC 1 cut(s) 640
AlwI GGATC 4 cut(s) 45, 84, 97, 894
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 5 cut(s) 327, 347, 369, 875, 1465
ApeKI GCWGC 7 cut(s) 491, 536, 575, 780, 1067, 1070, 1073
Asp700I GAANNNNTTC 1 cut(s) 516
AspLEI GCGC 4 cut(s) 64, 85, 145, 466
AspS9I GGNCC 5 cut(s) 108, 295, 348, 860, 1466
AsuC2I CCSGG 2 cut(s) 87, 1426
AsuHPI GGTGA 3 cut(s) 268, 652, 830
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 108, 295, 860
BalI TGGCCA 1 cut(s) 329
BamHI GGATCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 640
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 7 cut(s) 503, 523, 587, 792, 1054, 1060, 1082
BccI CCATC 6 cut(s) 431, 467, 675, 995, 1457, 1531
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 3 cut(s) 27, 879, 1401
BcnI CCSGG 2 cut(s) 87, 1426
BcuI ACTAGT 1 cut(s) 1481
BfaI CTAG 1 cut(s) 1482
BfmI CTRYAG 3 cut(s) 489, 972, 1068
BfoI RGCGCY 2 cut(s) 65, 86
BfuAI ACCTGC 2 cut(s) 739, 965
BglII AGATCT 1 cut(s) 550
Bme1390I CCNGG 5 cut(s) 27, 87, 879, 1401, 1426
Bme18I GGWCC 3 cut(s) 108, 295, 860
BmeRI GACNNNNNGTC 1 cut(s) 417
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 5 cut(s) 108, 295, 348, 860, 1466
BmiI GGNNCC 6 cut(s) 91, 297, 652, 862, 1170, 1468
BmrFI CCNGG 5 cut(s) 27, 87, 879, 1401, 1426
BmsI GCATC 6 cut(s) 707, 786, 1003, 1044, 1054, 1081
BoxI GACNNNNGTC 1 cut(s) 1044
BpiI GAAGAC 1 cut(s) 792
BpuEI CTTGAG 2 cut(s) 886, 1289
BpuMI CCSGG 2 cut(s) 87, 1426
BsaBI GATNNNNATC 2 cut(s) 285, 800
BsaJI CCNNGG 5 cut(s) 25, 507, 584, 1400, 1457
BsaWI WCCGGW 1 cut(s) 1060
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 426, 456
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 304, 737, 1406, 1463
Bse1I ACTGG 2 cut(s) 372, 844
Bse3DI GCAATG 2 cut(s) 155, 1172
Bse8I GATNNNNATC 2 cut(s) 285, 800
BseBI CCWGG 3 cut(s) 27, 879, 1401
BseDI CCNNGG 5 cut(s) 25, 507, 584, 1400, 1457
BseGI GGATG 9 cut(s) 686, 801, 1018, 1059, 1069, 1096, 1123, 1430, 1468
BseJI GATNNNNATC 2 cut(s) 285, 800
BseLI CCNNNNNNNGG 5 cut(s) 85, 304, 737, 1406, 1463
BseMI GCAATG 2 cut(s) 155, 1172
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 372, 844
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 7 cut(s) 503, 523, 587, 792, 1054, 1060, 1082
Bsh1236I CGCG 3 cut(s) 95, 143, 464
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 5 cut(s) 329, 349, 371, 877, 1467
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 640
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 3 cut(s) 86, 1061, 1425
BslFI GGGAC 3 cut(s) 281, 846, 1122
BslI CCNNNNNNNGG 5 cut(s) 85, 304, 737, 1406, 1463
BsmFI GGGAC 3 cut(s) 281, 846, 1122
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 5 cut(s) 329, 349, 371, 877, 1467
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 6 cut(s) 50, 89, 103, 550, 763, 899
BspANI GGCC 5 cut(s) 329, 349, 371, 877, 1467
BspCNI CTCAG 1 cut(s) 57
BspFNI CGCG 3 cut(s) 95, 143, 464
BspLI GGNNCC 6 cut(s) 91, 297, 652, 862, 1170, 1468
BspMAI CTGCAG 3 cut(s) 493, 976, 1072
BspMI ACCTGC 2 cut(s) 739, 965
BspPI GGATC 4 cut(s) 45, 84, 97, 894
BspQI GCTCTTC 2 cut(s) 167, 522
BsrDI GCAATG 2 cut(s) 155, 1172
BsrI ACTGG 2 cut(s) 372, 844
BssECI CCNNGG 5 cut(s) 25, 507, 584, 1400, 1457
BssMI GATC 6 cut(s) 50, 89, 103, 550, 763, 899
BssT1I CCWWGG 3 cut(s) 507, 584, 1457
Bst2UI CCWGG 3 cut(s) 27, 879, 1401
Bst4CI ACNGT 7 cut(s) 120, 295, 365, 658, 1043, 1252, 1282
Bst6I CTCTTC 2 cut(s) 167, 522
BstC8I GCNNGC 2 cut(s) 601, 1164
BstDEI CTNAG 1 cut(s) 65
BstENI CCTNNNNNAGG 1 cut(s) 735
BstF5I GGATG 9 cut(s) 686, 801, 1018, 1059, 1069, 1096, 1123, 1430, 1468
BstFNI CGCG 3 cut(s) 95, 143, 464
BstH2I RGCGCY 2 cut(s) 65, 86
BstHHI GCGC 4 cut(s) 64, 85, 145, 466
BstKTI GATC 6 cut(s) 53, 92, 106, 553, 766, 902
BstMBI GATC 6 cut(s) 50, 89, 103, 550, 763, 899
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 5 cut(s) 151, 1022, 1031, 1073, 1100
BstNI CCWGG 3 cut(s) 27, 879, 1401
BstNSI RCATGY 1 cut(s) 1357
BstPAI GACNNNNGTC 1 cut(s) 1044
BstSCI CCNGG 5 cut(s) 25, 85, 877, 1399, 1424
BstSFI CTRYAG 3 cut(s) 489, 972, 1068
BstUI CGCG 3 cut(s) 95, 143, 464
BstV1I GCAGC 7 cut(s) 503, 523, 587, 792, 1054, 1060, 1082
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 3 cut(s) 89, 550, 899
BstYI RGATCY 3 cut(s) 89, 550, 899
BsuRI GGCC 5 cut(s) 329, 349, 371, 877, 1467
BtsCI GGATG 9 cut(s) 686, 801, 1018, 1059, 1069, 1096, 1123, 1430, 1468
BtsIMutI CAGTG 1 cut(s) 654
BveI ACCTGC 2 cut(s) 739, 965
Cac8I GCNNGC 2 cut(s) 601, 1164
CfoI GCGC 4 cut(s) 64, 85, 145, 466
Cfr13I GGNCC 5 cut(s) 108, 295, 348, 860, 1466
Csp6I GTAC 2 cut(s) 1339, 1479
CviAII CATG 4 cut(s) 41, 215, 1216, 1354
CviQI GTAC 2 cut(s) 1339, 1479
DdeI CTNAG 1 cut(s) 65
DpnI GATC 6 cut(s) 52, 91, 105, 552, 765, 901
DpnII GATC 6 cut(s) 50, 89, 103, 550, 763, 899
DriI GACNNNNNGTC 1 cut(s) 417
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 167, 522
Eam1105I GACNNNNNGTC 1 cut(s) 417
EarI CTCTTC 2 cut(s) 167, 522
Ecl136II GAGCTC 1 cut(s) 638
Eco130I CCWWGG 3 cut(s) 507, 584, 1457
Eco24I GRGCYC 1 cut(s) 640
Eco47I GGWCC 3 cut(s) 108, 295, 860
Eco53kI GAGCTC 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 638
EcoNI CCTNNNNNAGG 1 cut(s) 735
EcoRII CCWGG 3 cut(s) 25, 877, 1399
EcoT14I CCWWGG 3 cut(s) 507, 584, 1457
EcoT38I GRGCYC 1 cut(s) 640
ErhI CCWWGG 3 cut(s) 507, 584, 1457
FaeI CATG 4 cut(s) 44, 218, 1219, 1357
FalI AAGNNNNNCTT 4 cut(s) 1020, 1052, 1251, 1283
FaqI GGGAC 3 cut(s) 281, 846, 1122
FatI CATG 4 cut(s) 40, 214, 1215, 1353
FauI CCCGC 1 cut(s) 306
FblI GTMKAC 1 cut(s) 968
FokI GGATG 9 cut(s) 693, 808, 1025, 1066, 1076, 1103, 1130, 1417, 1475
FriOI GRGCYC 1 cut(s) 640
FspBI CTAG 1 cut(s) 1482
GlaI GCGC 4 cut(s) 63, 84, 144, 465
HaeII RGCGCY 2 cut(s) 65, 86
HaeIII GGCC 5 cut(s) 329, 349, 371, 877, 1467
HapII CCGG 3 cut(s) 86, 1061, 1425
HhaI GCGC 4 cut(s) 64, 85, 145, 466
Hin1II CATG 4 cut(s) 44, 218, 1219, 1357
Hin6I GCGC 4 cut(s) 62, 83, 143, 464
HinP1I GCGC 4 cut(s) 62, 83, 143, 464
HinfI GANTC 4 cut(s) 207, 710, 1238, 1331
HpaII CCGG 3 cut(s) 86, 1061, 1425
HphI GGTGA 3 cut(s) 268, 652, 830
Hpy166II GTNNAC 1 cut(s) 969
Hpy188I TCNGA 4 cut(s) 108, 291, 378, 1321
Hpy188III TCNNGA 8 cut(s) 101, 610, 648, 897, 1037, 1115, 1268, 1527
Hpy8I GTNNAC 1 cut(s) 969
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 4 cut(s) 731, 1295, 1480, 1505
HpyCH4III ACNGT 7 cut(s) 120, 295, 365, 658, 1043, 1252, 1282
HpyCH4IV ACGT 4 cut(s) 10, 34, 644, 918
HpyCH4V TGCA 9 cut(s) 40, 491, 575, 698, 720, 780, 974, 1070, 1350
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 1022, 1031, 1073, 1100
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 4 cut(s) 10, 34, 644, 918
Hsp92II CATG 4 cut(s) 44, 218, 1219, 1357
HspAI GCGC 4 cut(s) 62, 83, 143, 464
Kzo9I GATC 6 cut(s) 50, 89, 103, 550, 763, 899
LguI GCTCTTC 2 cut(s) 167, 522
LmnI GCTCC 1 cut(s) 1174
Lsp1109I GCAGC 7 cut(s) 503, 523, 587, 792, 1054, 1060, 1082
LweI GCATC 6 cut(s) 707, 786, 1003, 1044, 1054, 1081
MaeI CTAG 1 cut(s) 1482
MaeII ACGT 4 cut(s) 10, 34, 644, 918
MaeIII GTNAC 3 cut(s) 188, 658, 1538
MalI GATC 6 cut(s) 52, 91, 105, 552, 765, 901
MboI GATC 6 cut(s) 50, 89, 103, 550, 763, 899
MboII GAAGA 7 cut(s) 154, 349, 509, 797, 1040, 1236, 1507
MflI RGATCY 3 cut(s) 89, 550, 899
MhlI GDGCHC 1 cut(s) 640
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 2 cut(s) 323, 669
MluNI TGGCCA 1 cut(s) 329
MmeI TCCRAC 1 cut(s) 294
Mox20I TGGCCA 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 516
MscI TGGCCA 1 cut(s) 329
MseI TTAA 4 cut(s) 555, 1209, 1221, 1359
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 3 cut(s) 86, 1061, 1425
MspR9I CCNGG 5 cut(s) 27, 87, 879, 1401, 1426
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 3 cut(s) 27, 879, 1401
MvnI CGCG 3 cut(s) 95, 143, 464
MwoI GCNNNNNNNGC 5 cut(s) 151, 1022, 1031, 1073, 1100
NciI CCSGG 2 cut(s) 87, 1426
NdeII GATC 6 cut(s) 50, 89, 103, 550, 763, 899
NlaIII CATG 4 cut(s) 44, 218, 1219, 1357
NlaIV GGNNCC 6 cut(s) 91, 297, 652, 862, 1170, 1468
NmuCI GTSAC 2 cut(s) 658, 1538
NspI RCATGY 1 cut(s) 1357
PaqCI CACCTGC 1 cut(s) 965
PciI ACATGT 1 cut(s) 1353
PciSI GCTCTTC 2 cut(s) 167, 522
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 4 cut(s) 207, 710, 1238, 1331
Ple19I CGATCG 1 cut(s) 106
PscI ACATGT 1 cut(s) 1353
PshAI GACNNNNGTC 1 cut(s) 1044
Psp124BI GAGCTC 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 25, 877, 1399
PspGI CCWGG 3 cut(s) 25, 877, 1399
PspN4I GGNNCC 6 cut(s) 91, 297, 652, 862, 1170, 1468
PspPI GGNCC 5 cut(s) 108, 295, 348, 860, 1466
PstI CTGCAG 3 cut(s) 493, 976, 1072
PsuI RGATCY 3 cut(s) 89, 550, 899
PvuI CGATCG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 2 cut(s) 1340, 1480
RsaNI GTAC 2 cut(s) 1339, 1479
SacI GAGCTC 1 cut(s) 640
SapI GCTCTTC 2 cut(s) 167, 522
SaqAI TTAA 4 cut(s) 555, 1209, 1221, 1359
Sau3AI GATC 6 cut(s) 50, 89, 103, 550, 763, 899
Sau96I GGNCC 5 cut(s) 108, 295, 348, 860, 1466
ScrFI CCNGG 5 cut(s) 27, 87, 879, 1401, 1426
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 6 cut(s) 707, 786, 1003, 1044, 1054, 1081
SfcI CTRYAG 3 cut(s) 489, 972, 1068
SinI GGWCC 3 cut(s) 108, 295, 860
SmlI CTYRAG 2 cut(s) 865, 1268
SmoI CTYRAG 2 cut(s) 865, 1268
SpeI ACTAGT 1 cut(s) 1481
Sse9I AATT 2 cut(s) 323, 669
SspI AATATT 2 cut(s) 529, 1293
SspMI CTAG 1 cut(s) 1482
SstI GAGCTC 1 cut(s) 640
StyD4I CCNGG 5 cut(s) 25, 85, 877, 1399, 1424
StyI CCWWGG 3 cut(s) 507, 584, 1457
TaaI ACNGT 7 cut(s) 120, 295, 365, 658, 1043, 1252, 1282
TaiI ACGT 4 cut(s) 13, 37, 647, 921
TaqI TCGA 2 cut(s) 49, 102
TasI AATT 2 cut(s) 323, 669
TatI WGTACW 1 cut(s) 1478
TauI GCSGC 4 cut(s) 62, 138, 497, 1169
TfiI GAWTC 4 cut(s) 207, 710, 1238, 1331
Tru1I TTAA 4 cut(s) 555, 1209, 1221, 1359
Tru9I TTAA 4 cut(s) 555, 1209, 1221, 1359
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 2 cut(s) 658, 1538
TseI GCWGC 7 cut(s) 491, 536, 575, 780, 1067, 1070, 1073
Tsp45I GTSAC 2 cut(s) 658, 1538
TspDTI ATGAA 4 cut(s) 579, 919, 974, 1251
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 1 cut(s) 661
VpaK11BI GGWCC 3 cut(s) 108, 295, 860
XagI CCTNNNNNAGG 1 cut(s) 735
XceI RCATGY 1 cut(s) 1357
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
XmiI GTMKAC 1 cut(s) 968
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.