Rroxscaffold_7G00160920

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009593
Physical Location & Seq
Reverse (-)
3785185 .. 3789273
4089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160920.1

Sequence Viewer

Length: 1293 bp
ATGTCCAACAGGAGTGATAATCATCAGACGGTCCCGCTTTCGGTGTTGCTAAAGCGAGAATTGGCCAATGAGAAGATTGAGAGGCCCGAGATTATATACGGTCAGGCCAGTCAGAGCAAGAAAGGGGAGGATTTCACTATGGTAAAGACGGATTGTCAAAGAGTTGTGGGAGATGGAGTTTCTACCTATTCAGTTTTCGCGCTATTTGACGGGCATAACGGGTCTGCAGCCGCTTTATACTCCAAGGAAGCTCTTCTAAACAATATTTTAGCTGCTATCCCCACAGATCTTAACAGAGATGAATGGATTGCAGCTTTGCCAAGGTCGTTGGTAGCAGGCTTTGTCAAGACAGACAAAGATTTCCAAGAGAGAGCTCAAACGTCAGGAACCACTGTCACCTTTGTAATTATAGAGGGATGGGTAATATCTGTTGCATCGGTTGGCGATTCTCGTTGCATAGTTGAACCTGCTGAAGGTGGAGTTTATTATTTATCAGCAGATCATAGGCTTGAATGCAGCGAAGACGAGAGGATGCGTATCACTTCAAGTGGTGGTGAGGTTGGTCGGCTGAATACTGGCGGTGGGGCAGAGATTGGTCCCTTGAGGTGTTGGCCTGGTGGTTTGTGTCTTTCAAGATCCATTGGGGATATGGACGTAGGCGAGTTCATTGTTCCTGTTCCTTATGTAAAGCAAATGAAGTTGTCTACTGCAGGTGGTAGGGTAATCATATCAAGTGATGGTGTATGGGATGCTCTGTCCGCAGAAGAAGCTCTTGACTGTTGTCGTGGGATGCCACCGGATGCTGCAGCTGCCCAAGTAGTCAAGGATGCTGTTGGGGCAAAGGGACTTCGGGATGATACAACCTGTATTGTGATTGACATTTTACCACAGGAGAAGCCAGCGGCTCCATTGCCGCCACCAAAGAGGCAAGGAAAAGGAGTTTTTAAGTCCATGTTTAAGAAAAAGCCTAATGAATCATCTTCTACTGTTGAAAAAGAATATCTTGAGCCAGATACGGTGGAGGAAATATTTGAGGAAGGTTCTGCTTCTCTTTCCGAAAGGTTAGATTCAAAGTACCCACTCTGCAACATGTTTAAGCTCTTTATGTGTGCTGTTTGTCAAGTTGAAATCAAACCAGGGGAGGGTATTTCAATACACTCCGGGTCATCCTCTAACCCAGAAAAAGTGCGACCTTGGGATGGCCCCTTCCTTTGTACTAGTTGCCAAGAGAAGAAAGAGGCTATGGAAGGGAAAAGACCATCAGGAAATCGGCGTGACAGCGATAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

430

Amino Acids

46.6

Weight (kDa)

5.07

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 58 - 277 1.6e-28 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 701
Acc36I ACCTGC 2 cut(s) 475, 701
AccI GTMKAC 1 cut(s) 704
AccII CGCG 1 cut(s) 200
AciI CCGC 6 cut(s) 35, 231, 579, 759, 902, 914
AclWI GGATC 1 cut(s) 630
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 2 cut(s) 1076, 1216
AfiI CCNNNNNNNGG 4 cut(s) 40, 473, 1142, 1199
AflIII ACRYGT 1 cut(s) 1089
AgsI TTSAA 8 cut(s) 464, 512, 546, 633, 992, 1071, 1127, 1152
AhdI GACNNNNNGTC 1 cut(s) 153
AhlI ACTAGT 1 cut(s) 1217
AjnI CCWGG 2 cut(s) 613, 1135
AluBI AGCT 7 cut(s) 251, 272, 314, 374, 770, 809, 1099
AluI AGCT 7 cut(s) 251, 272, 314, 374, 770, 809, 1099
Alw21I GWGCWC 1 cut(s) 376
AlwI GGATC 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 5 cut(s) 63, 83, 105, 611, 1201
ApeKI GCWGC 7 cut(s) 227, 272, 311, 516, 803, 806, 809
Asp700I GAANNNNTTC 1 cut(s) 252
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 4 cut(s) 31, 84, 596, 1202
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 2 cut(s) 388, 566
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 2 cut(s) 31, 596
BalI TGGCCA 1 cut(s) 65
BanII GRGCYC 1 cut(s) 376
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 7 cut(s) 239, 259, 323, 528, 790, 796, 818
BccI CCATC 5 cut(s) 167, 411, 731, 1193, 1267
BciT130I CCWGG 2 cut(s) 615, 1137
BcnI CCSGG 1 cut(s) 1162
BcuI ACTAGT 1 cut(s) 1217
BfaI CTAG 1 cut(s) 1218
BfmI CTRYAG 3 cut(s) 225, 708, 804
BfuAI ACCTGC 2 cut(s) 475, 701
BglII AGATCT 1 cut(s) 286
Bme1390I CCNGG 3 cut(s) 615, 1137, 1162
Bme18I GGWCC 2 cut(s) 31, 596
BmeRI GACNNNNNGTC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 4 cut(s) 31, 84, 596, 1202
BmiI GGNNCC 5 cut(s) 33, 388, 598, 906, 1204
BmrFI CCNGG 3 cut(s) 615, 1137, 1162
BmsI GCATC 6 cut(s) 443, 522, 739, 780, 790, 817
BoxI GACNNNNGTC 1 cut(s) 780
BpiI GAAGAC 1 cut(s) 528
BpuEI CTTGAG 2 cut(s) 622, 1025
BpuMI CCSGG 1 cut(s) 1162
BsaBI GATNNNNATC 2 cut(s) 21, 536
BsaJI CCNNGG 4 cut(s) 243, 320, 1136, 1193
BsaWI WCCGGW 1 cut(s) 796
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 473, 1142, 1199
Bse1I ACTGG 2 cut(s) 108, 580
Bse3DI GCAATG 1 cut(s) 908
Bse8I GATNNNNATC 2 cut(s) 21, 536
BseBI CCWGG 2 cut(s) 615, 1137
BseDI CCNNGG 4 cut(s) 243, 320, 1136, 1193
BseGI GGATG 9 cut(s) 422, 537, 754, 795, 805, 832, 859, 1166, 1204
BseJI GATNNNNATC 2 cut(s) 21, 536
BseLI CCNNNNNNNGG 4 cut(s) 40, 473, 1142, 1199
BseMI GCAATG 1 cut(s) 908
BseNI ACTGG 2 cut(s) 108, 580
BseXI GCAGC 7 cut(s) 239, 259, 323, 528, 790, 796, 818
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 5 cut(s) 65, 85, 107, 613, 1203
BsiHKAI GWGCWC 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 2 cut(s) 797, 1161
BslFI GGGAC 3 cut(s) 17, 582, 858
BslI CCNNNNNNNGG 4 cut(s) 40, 473, 1142, 1199
BsmFI GGGAC 3 cut(s) 17, 582, 858
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 5 cut(s) 65, 85, 107, 613, 1203
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 3 cut(s) 286, 499, 635
BspACI CCGC 6 cut(s) 35, 231, 579, 759, 902, 914
BspANI GGCC 5 cut(s) 65, 85, 107, 613, 1203
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 5 cut(s) 33, 388, 598, 906, 1204
BspMAI CTGCAG 3 cut(s) 229, 712, 808
BspMI ACCTGC 2 cut(s) 475, 701
BspPI GGATC 1 cut(s) 630
BspQI GCTCTTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 908
BsrI ACTGG 2 cut(s) 108, 580
BssECI CCNNGG 4 cut(s) 243, 320, 1136, 1193
BssMI GATC 3 cut(s) 286, 499, 635
BssT1I CCWWGG 3 cut(s) 243, 320, 1193
Bst2UI CCWGG 2 cut(s) 615, 1137
Bst4CI ACNGT 6 cut(s) 31, 101, 394, 779, 988, 1018
Bst6I CTCTTC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 337, 900
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 9 cut(s) 422, 537, 754, 795, 805, 832, 859, 1166, 1204
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 3 cut(s) 289, 502, 638
BstMBI GATC 3 cut(s) 286, 499, 635
BstMWI GCNNNNNNNGC 4 cut(s) 758, 767, 809, 836
BstNI CCWGG 2 cut(s) 615, 1137
BstNSI RCATGY 1 cut(s) 1093
BstPAI GACNNNNGTC 1 cut(s) 780
BstSCI CCNGG 3 cut(s) 613, 1135, 1160
BstSFI CTRYAG 3 cut(s) 225, 708, 804
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 7 cut(s) 239, 259, 323, 528, 790, 796, 818
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 2 cut(s) 286, 635
BstYI RGATCY 2 cut(s) 286, 635
BsuRI GGCC 5 cut(s) 65, 85, 107, 613, 1203
BtsCI GGATG 9 cut(s) 422, 537, 754, 795, 805, 832, 859, 1166, 1204
BtsIMutI CAGTG 1 cut(s) 390
BveI ACCTGC 2 cut(s) 475, 701
Cac8I GCNNGC 2 cut(s) 337, 900
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 4 cut(s) 31, 84, 596, 1202
Csp6I GTAC 2 cut(s) 1075, 1215
CviAII CATG 2 cut(s) 952, 1090
CviQI GTAC 2 cut(s) 1075, 1215
DpnI GATC 3 cut(s) 288, 501, 637
DpnII GATC 3 cut(s) 286, 499, 635
DriI GACNNNNNGTC 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 258
Eam1105I GACNNNNNGTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 374
Eco130I CCWWGG 3 cut(s) 243, 320, 1193
Eco24I GRGCYC 1 cut(s) 376
Eco47I GGWCC 2 cut(s) 31, 596
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 374
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 613, 1135
EcoT14I CCWWGG 3 cut(s) 243, 320, 1193
EcoT38I GRGCYC 1 cut(s) 376
ErhI CCWWGG 3 cut(s) 243, 320, 1193
FaeI CATG 2 cut(s) 955, 1093
FalI AAGNNNNNCTT 4 cut(s) 756, 788, 987, 1019
FaqI GGGAC 3 cut(s) 17, 582, 858
FatI CATG 2 cut(s) 951, 1089
FauI CCCGC 1 cut(s) 42
FblI GTMKAC 1 cut(s) 704
FokI GGATG 9 cut(s) 429, 544, 761, 802, 812, 839, 866, 1153, 1211
FriOI GRGCYC 1 cut(s) 376
FspBI CTAG 1 cut(s) 1218
GlaI GCGC 1 cut(s) 201
HaeIII GGCC 5 cut(s) 65, 85, 107, 613, 1203
HapII CCGG 2 cut(s) 797, 1161
HhaI GCGC 1 cut(s) 202
Hin1II CATG 2 cut(s) 955, 1093
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 3 cut(s) 446, 974, 1067
HpaII CCGG 2 cut(s) 797, 1161
HphI GGTGA 2 cut(s) 388, 566
Hpy166II GTNNAC 1 cut(s) 705
Hpy188I TCNGA 3 cut(s) 27, 114, 1057
Hpy188III TCNNGA 7 cut(s) 346, 384, 633, 773, 851, 1004, 1263
Hpy8I GTNNAC 1 cut(s) 705
HpyAV CCTTC 4 cut(s) 467, 1031, 1216, 1241
HpyCH4III ACNGT 6 cut(s) 31, 101, 394, 779, 988, 1018
HpyCH4IV ACGT 2 cut(s) 380, 654
HpyCH4V TGCA 8 cut(s) 227, 311, 434, 456, 516, 710, 806, 1086
HpyF10VI GCNNNNNNNGC 4 cut(s) 758, 767, 809, 836
HpySE526I ACGT 2 cut(s) 380, 654
Hsp92II CATG 2 cut(s) 955, 1093
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 3 cut(s) 286, 499, 635
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 910
Lsp1109I GCAGC 7 cut(s) 239, 259, 323, 528, 790, 796, 818
LweI GCATC 6 cut(s) 443, 522, 739, 780, 790, 817
MaeI CTAG 1 cut(s) 1218
MaeII ACGT 2 cut(s) 380, 654
MaeIII GTNAC 2 cut(s) 394, 1274
MalI GATC 3 cut(s) 288, 501, 637
MboI GATC 3 cut(s) 286, 499, 635
MboII GAAGA 6 cut(s) 85, 245, 533, 776, 972, 1243
MflI RGATCY 2 cut(s) 286, 635
MhlI GDGCHC 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 2 cut(s) 59, 405
MluNI TGGCCA 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 30
Mox20I TGGCCA 1 cut(s) 65
MroXI GAANNNNTTC 1 cut(s) 252
MscI TGGCCA 1 cut(s) 65
MseI TTAA 4 cut(s) 291, 945, 957, 1095
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 809, 902
MspI CCGG 2 cut(s) 797, 1161
MspR9I CCNGG 3 cut(s) 615, 1137, 1162
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 2 cut(s) 615, 1137
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 4 cut(s) 758, 767, 809, 836
NciI CCSGG 1 cut(s) 1162
NdeII GATC 3 cut(s) 286, 499, 635
NlaIII CATG 2 cut(s) 955, 1093
NlaIV GGNNCC 5 cut(s) 33, 388, 598, 906, 1204
NmuCI GTSAC 2 cut(s) 394, 1274
NspI RCATGY 1 cut(s) 1093
PaqCI CACCTGC 1 cut(s) 701
PciI ACATGT 1 cut(s) 1089
PciSI GCTCTTC 1 cut(s) 258
PctI GAATGC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 3 cut(s) 446, 974, 1067
PscI ACATGT 1 cut(s) 1089
PshAI GACNNNNGTC 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 376
Psp6I CCWGG 2 cut(s) 613, 1135
PspGI CCWGG 2 cut(s) 613, 1135
PspN4I GGNNCC 5 cut(s) 33, 388, 598, 906, 1204
PspPI GGNCC 4 cut(s) 31, 84, 596, 1202
PstI CTGCAG 3 cut(s) 229, 712, 808
PsuI RGATCY 2 cut(s) 286, 635
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 2 cut(s) 1076, 1216
RsaNI GTAC 2 cut(s) 1075, 1215
SacI GAGCTC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 4 cut(s) 291, 945, 957, 1095
Sau3AI GATC 3 cut(s) 286, 499, 635
Sau96I GGNCC 4 cut(s) 31, 84, 596, 1202
ScrFI CCNGG 3 cut(s) 615, 1137, 1162
SduI GDGCHC 1 cut(s) 376
SfaNI GCATC 6 cut(s) 443, 522, 739, 780, 790, 817
SfcI CTRYAG 3 cut(s) 225, 708, 804
SinI GGWCC 2 cut(s) 31, 596
SmlI CTYRAG 2 cut(s) 601, 1004
SmoI CTYRAG 2 cut(s) 601, 1004
SpeI ACTAGT 1 cut(s) 1217
Sse9I AATT 2 cut(s) 59, 405
SsiI CCGC 6 cut(s) 35, 231, 579, 759, 902, 914
SspI AATATT 2 cut(s) 265, 1029
SspMI CTAG 1 cut(s) 1218
SstI GAGCTC 1 cut(s) 376
StyD4I CCNGG 3 cut(s) 613, 1135, 1160
StyI CCWWGG 3 cut(s) 243, 320, 1193
TaaI ACNGT 6 cut(s) 31, 101, 394, 779, 988, 1018
TaiI ACGT 2 cut(s) 383, 657
TasI AATT 2 cut(s) 59, 405
TatI WGTACW 1 cut(s) 1214
TauI GCSGC 3 cut(s) 233, 905, 916
TfiI GAWTC 3 cut(s) 446, 974, 1067
Tru1I TTAA 4 cut(s) 291, 945, 957, 1095
Tru9I TTAA 4 cut(s) 291, 945, 957, 1095
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 2 cut(s) 394, 1274
TseI GCWGC 7 cut(s) 227, 272, 311, 516, 803, 806, 809
Tsp45I GTSAC 2 cut(s) 394, 1274
TspDTI ATGAA 4 cut(s) 315, 655, 710, 987
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 2 cut(s) 31, 596
XagI CCTNNNNNAGG 1 cut(s) 471
XceI RCATGY 1 cut(s) 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 704
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.