MD15G1084200.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009593
Physical Location & Seq
Reverse (-)
5823427 .. 5828633
5207 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1084200.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGTCCACTAAGAGCGAAAATCATCAAACTGTTCCGCTTTCGGTGCTGCTCAAGCGTGAATTGGCCAATGAGAAGATTGAGAGACCGGAGATTATACACGGTCAGGCGAGTCAGAGCAAGAAAGGAGAGGATTTCACGTTGGTAAAGACGGAATGTCAAAGAATTGTGGGAGATGGAGTTTCTACGTATTCAGTTTTCGGGCTATTTGACGGGCATAATGGGTCAGCAGCCGCTATTTACTCCAAGGAGAATCTTCTAACTAATGTTTTGGCTGCTATCCCTTCAGATCTAAATAGAGATGAATGGGTAGCAGCATTGCCAAGGGCTTTTGTAGCAGGCTTCGTCAAAACAGACAAAGAATTCCAAGAGAGATCTCAAACGTCAGGAACAACTGTCACGTTTGTGATTATAGAAGGATGGGTCATATCTGTTGCATCTGTCGGCGATTCTCGTTGCATAGTTGAACCTGCTGAAGGTGGAGTTTACTATTTATCAGCAGATCATAGGCTTGAAATCAATGAAGAGGAGAGACAGCGTATCACCTCAAGTGGGGGCGAAGTTGGTCGGCTTAATACTGGTGGTGGGGCAGAGATCGGTCCTTTGAGGTGTTGGCCTGGTGGCTTGTGTCTATCACGATCCATTGGTGATATGGACGTGGGGGAGTTCATTGTTCCTGTTCCTTATGTAAAGCAAATCAAGTTGTCAACCGCAGGTGGCAGACTTATCATATCAAGCGATGGTGTCTGGGATGCTTTGTCTGCAGAAGAAGCTCTTGATTGTTGCCGTGGGATGCCACCGGACGCTGCAGCTGCACAAATTGTAAAGGATGCTGTAGGGCATAAGGGACTTCGGGATGATACAACCTGCATTGTGATCGATATTTTACCACAAGAGAAGCCACAAGCTTCGTTACCCCAACCAAAGAGGCAGGGAAAAGGAGTGTTGTTTAAATCCATGTTTAAGAAAAAATCATCTGAATCATCTTCTAGTGTTGAAAAAGAATATATTGAGCCAGATGAGGTGGAGGAGTTATTTGAGGAAGGTTCTGCTTCGCTTTCTGAAAGGTTAGATACAAAATACCCGCTCTGCAACATGTTCAAATTGTTTATGTGTGCAGTTTGTCAAGTAGAAATTAAACCAGGAGAGGGTATTTCGATACACGCTGGATCATCGAACCCAGGAAAATTGCGACCTTGGGATGGCCCCTTCCTCTGCTCGAGTTGCCAAGGGAAAAAAGAGGCTATGGAGGGGAAGAGACCATCAGGAAGAAGACATGATAGCGATAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

431

Amino Acids

46.66

Weight (kDa)

5.29

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 58 - 274 9.3e-30 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 701
Acc36I ACCTGC 3 cut(s) 475, 701, 872
AccBSI CCGCTC 1 cut(s) 1084
AciI CCGC 4 cut(s) 35, 231, 708, 1082
AclWI GGATC 2 cut(s) 630, 1174
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 267, 492
AfiI CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 4 cut(s) 464, 512, 995, 1099
AhdI GACNNNNNGTC 1 cut(s) 153
AjiI CACGTC 1 cut(s) 655
AjnI CCWGG 3 cut(s) 613, 1138, 1177
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 3 cut(s) 770, 809, 905
AluI AGCT 3 cut(s) 770, 809, 905
Alw26I GTCTC 3 cut(s) 76, 523, 1249
AlwI GGATC 2 cut(s) 630, 1174
Ama87I CYCGRG 1 cut(s) 1216
AoxI GGCC 3 cut(s) 63, 611, 1201
ApeKI GCWGC 7 cut(s) 46, 227, 272, 311, 803, 806, 809
ApoI RAATTY 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 596, 1202
AsuHPI GGTGA 2 cut(s) 532, 656
AvaI CYCGRG 1 cut(s) 1216
AvaII GGWCC 1 cut(s) 596
BalI TGGCCA 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 1276
BbvI GCAGC 7 cut(s) 33, 239, 259, 323, 790, 796, 818
BccI CCATC 5 cut(s) 167, 411, 731, 1193, 1267
BceAI ACGGC 1 cut(s) 768
BcgI CGANNNNNNTGC 2 cut(s) 856, 890
BciT130I CCWGG 3 cut(s) 615, 1140, 1179
BcoDI GTCTC 3 cut(s) 76, 523, 1249
BfaI CTAG 1 cut(s) 987
BfmI CTRYAG 3 cut(s) 759, 804, 831
BfuAI ACCTGC 3 cut(s) 475, 701, 872
BglII AGATCT 2 cut(s) 286, 371
BisI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 804, 807, 810
BlsI GCNGC 8 cut(s) 48, 229, 232, 274, 313, 805, 808, 811
Bme1390I CCNGG 3 cut(s) 615, 1140, 1179
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 1216
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 596, 1202
BmiI GGNNCC 1 cut(s) 1204
BmrFI CCNGG 3 cut(s) 615, 1140, 1179
BmsI GCATC 4 cut(s) 443, 739, 780, 817
BpiI GAAGAC 1 cut(s) 1276
BpuEI CTTGAG 2 cut(s) 35, 529
Bsa29I ATCGAT 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 186
BsaI GGTCTC 2 cut(s) 76, 1249
BsaJI CCNNGG 6 cut(s) 243, 320, 784, 1177, 1193, 1225
BsaWI WCCGGW 2 cut(s) 85, 796
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
Bse1I ACTGG 1 cut(s) 580
Bse3DI GCAATG 1 cut(s) 314
BseBI CCWGG 3 cut(s) 615, 1140, 1179
BseCI ATCGAT 1 cut(s) 876
BseDI CCNNGG 6 cut(s) 243, 320, 784, 1177, 1193, 1225
BseGI GGATG 6 cut(s) 422, 754, 795, 832, 859, 1204
BseLI CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
BseMI GCAATG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 2 cut(s) 539, 1040
BseXI GCAGC 7 cut(s) 33, 239, 259, 323, 790, 796, 818
BsgI GTGCAG 2 cut(s) 795, 1134
BshFI GGCC 3 cut(s) 65, 613, 1203
BshVI ATCGAT 1 cut(s) 876
BsiHKCI CYCGRG 1 cut(s) 1216
BsiSI CCGG 2 cut(s) 86, 797
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 4 cut(s) 473, 549, 1145, 1199
BsmAI GTCTC 3 cut(s) 76, 523, 1249
BsmFI GGGAC 1 cut(s) 858
BsnI GGCC 3 cut(s) 65, 613, 1203
Bso31I GGTCTC 2 cut(s) 76, 1249
BsoBI CYCGRG 1 cut(s) 1216
Bsp143I GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
BspACI CCGC 4 cut(s) 35, 231, 708, 1082
BspANI GGCC 3 cut(s) 65, 613, 1203
BspDI ATCGAT 1 cut(s) 876
BspLI GGNNCC 1 cut(s) 1204
BspMAI CTGCAG 2 cut(s) 763, 808
BspMI ACCTGC 3 cut(s) 475, 701, 872
BspPI GGATC 2 cut(s) 630, 1174
BspTNI GGTCTC 2 cut(s) 76, 1249
BsrBI CCGCTC 1 cut(s) 1084
BsrDI GCAATG 1 cut(s) 314
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 6 cut(s) 243, 320, 784, 1177, 1193, 1225
BssMI GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
BssT1I CCWWGG 4 cut(s) 243, 320, 1193, 1225
Bst2UI CCWGG 3 cut(s) 615, 1140, 1179
Bst4CI ACNGT 3 cut(s) 31, 101, 394
Bst6I CTCTTC 2 cut(s) 516, 1247
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 784
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 6 cut(s) 422, 754, 795, 832, 859, 1204
BstKTI GATC 7 cut(s) 289, 374, 502, 594, 638, 876, 1169
BstMAI GTCTC 3 cut(s) 76, 523, 1249
BstMBI GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
BstMWI GCNNNNNNNGC 7 cut(s) 43, 52, 332, 758, 767, 809, 1221
BstNI CCWGG 3 cut(s) 615, 1140, 1179
BstNSI RCATGY 1 cut(s) 1096
BstSCI CCNGG 3 cut(s) 613, 1138, 1177
BstSFI CTRYAG 3 cut(s) 759, 804, 831
BstSNI TACGTA 1 cut(s) 186
BstV1I GCAGC 7 cut(s) 33, 239, 259, 323, 790, 796, 818
BstV2I GAAGAC 1 cut(s) 1276
BstX2I RGATCY 2 cut(s) 286, 371
BstYI RGATCY 2 cut(s) 286, 371
Bsu15I ATCGAT 1 cut(s) 876
BsuRI GGCC 3 cut(s) 65, 613, 1203
BsuTUI ATCGAT 1 cut(s) 876
BtgI CCRYGG 1 cut(s) 784
BtgZI GCGATG 1 cut(s) 750
BtrI CACGTC 1 cut(s) 655
BtsCI GGATG 6 cut(s) 422, 754, 795, 832, 859, 1204
BveI ACCTGC 3 cut(s) 475, 701, 872
Cac8I GCNNGC 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 596, 1202
ClaI ATCGAT 1 cut(s) 876
CseI GACGC 1 cut(s) 809
CviAII CATG 3 cut(s) 955, 1093, 1274
DdeI CTNAG 1 cut(s) 9
DpnI GATC 7 cut(s) 288, 373, 501, 593, 637, 875, 1168
DpnII GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
DraI TTTAAA 1 cut(s) 949
DriI GACNNNNNGTC 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 516, 1247
Eam1105I GACNNNNNGTC 1 cut(s) 153
EarI CTCTTC 2 cut(s) 516, 1247
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 4 cut(s) 243, 320, 1193, 1225
Eco31I GGTCTC 2 cut(s) 76, 1249
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 267, 492
Eco88I CYCGRG 1 cut(s) 1216
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 613, 1138, 1177
EcoT14I CCWWGG 4 cut(s) 243, 320, 1193, 1225
ErhI CCWWGG 4 cut(s) 243, 320, 1193, 1225
FaeI CATG 3 cut(s) 958, 1096, 1277
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 858
FatI CATG 3 cut(s) 954, 1092, 1273
FauI CCCGC 1 cut(s) 1089
Fnu4HI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 804, 807, 810
FokI GGATG 6 cut(s) 429, 761, 802, 839, 866, 1211
Fsp4HI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 804, 807, 810
FspBI CTAG 1 cut(s) 987
GluI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 804, 807, 810
HaeIII GGCC 3 cut(s) 65, 613, 1203
HapII CCGG 2 cut(s) 86, 797
HgaI GACGC 1 cut(s) 809
Hin1II CATG 3 cut(s) 958, 1096, 1277
HincII GTYRAC 1 cut(s) 705
HindII GTYRAC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 903
HinfI GANTC 4 cut(s) 109, 250, 446, 977
HpaII CCGG 2 cut(s) 86, 797
HphI GGTGA 2 cut(s) 532, 656
Hpy166II GTNNAC 3 cut(s) 6, 484, 705
Hpy188I TCNGA 4 cut(s) 114, 286, 976, 1060
Hpy188III TCNNGA 5 cut(s) 384, 633, 773, 851, 1263
Hpy8I GTNNAC 3 cut(s) 6, 484, 705
HpyAV CCTTC 5 cut(s) 291, 407, 467, 1034, 1216
HpyCH4III ACNGT 3 cut(s) 31, 101, 394
HpyCH4IV ACGT 5 cut(s) 137, 185, 380, 398, 654
HpyCH4V TGCA 8 cut(s) 434, 456, 761, 806, 812, 867, 1089, 1115
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 52, 332, 758, 767, 809, 1221
HpyF3I CTNAG 1 cut(s) 9
HpySE526I ACGT 5 cut(s) 137, 185, 380, 398, 654
Hsp92II CATG 3 cut(s) 958, 1096, 1277
Kzo9I GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
Lsp1109I GCAGC 7 cut(s) 33, 239, 259, 323, 790, 796, 818
LweI GCATC 4 cut(s) 443, 739, 780, 817
MaeI CTAG 1 cut(s) 987
MaeII ACGT 5 cut(s) 137, 185, 380, 398, 654
MaeIII GTNAC 2 cut(s) 394, 909
MalI GATC 7 cut(s) 288, 373, 501, 593, 637, 875, 1168
MbiI CCGCTC 1 cut(s) 1084
MboI GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
MboII GAAGA 8 cut(s) 85, 245, 533, 776, 975, 1264, 1278, 1281
MflI RGATCY 2 cut(s) 286, 371
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 7 cut(s) 59, 162, 359, 816, 1100, 1131, 1184
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 118
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 4 cut(s) 570, 948, 960, 1134
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 2 cut(s) 86, 797
MspR9I CCNGG 3 cut(s) 615, 1140, 1179
MvaI CCWGG 3 cut(s) 615, 1140, 1179
MwoI GCNNNNNNNGC 7 cut(s) 43, 52, 332, 758, 767, 809, 1221
NdeII GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
NlaIII CATG 3 cut(s) 958, 1096, 1277
NlaIV GGNNCC 1 cut(s) 1204
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 1096
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 1216
PaqCI CACCTGC 1 cut(s) 701
PciI ACATGT 1 cut(s) 1092
PfeI GAWTC 3 cut(s) 250, 446, 977
PkrI GCNGC 8 cut(s) 48, 229, 232, 274, 313, 805, 808, 811
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 186
PscI ACATGT 1 cut(s) 1092
Psp6I CCWGG 3 cut(s) 613, 1138, 1177
PspGI CCWGG 3 cut(s) 613, 1138, 1177
PspN4I GGNNCC 1 cut(s) 1204
PspPI GGNCC 2 cut(s) 596, 1202
PspXI VCTCGAGB 1 cut(s) 1216
PstI CTGCAG 2 cut(s) 763, 808
PsuI RGATCY 2 cut(s) 286, 371
PvuII CAGCTG 1 cut(s) 809
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 4 cut(s) 570, 948, 960, 1134
SatI GCNGC 8 cut(s) 47, 228, 231, 273, 312, 804, 807, 810
Sau3AI GATC 7 cut(s) 286, 371, 499, 591, 635, 873, 1166
Sau96I GGNCC 2 cut(s) 596, 1202
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 3 cut(s) 615, 1140, 1179
SfaNI GCATC 4 cut(s) 443, 739, 780, 817
SfcI CTRYAG 3 cut(s) 759, 804, 831
Sfr274I CTCGAG 1 cut(s) 1216
SinI GGWCC 1 cut(s) 596
SlaI CTCGAG 1 cut(s) 1216
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 3 cut(s) 50, 544, 1216
SmoI CTYRAG 3 cut(s) 50, 544, 1216
SnaBI TACGTA 1 cut(s) 186
Sse9I AATT 7 cut(s) 59, 162, 359, 816, 1100, 1131, 1184
SsiI CCGC 4 cut(s) 35, 231, 708, 1082
SspMI CTAG 1 cut(s) 987
StyD4I CCNGG 3 cut(s) 613, 1138, 1177
StyI CCWWGG 4 cut(s) 243, 320, 1193, 1225
TaaI ACNGT 3 cut(s) 31, 101, 394
TaiI ACGT 5 cut(s) 140, 188, 383, 401, 657
TaqI TCGA 4 cut(s) 876, 1154, 1172, 1217
TaqII GACCGA 1 cut(s) 584
TasI AATT 7 cut(s) 59, 162, 359, 816, 1100, 1131, 1184
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 3 cut(s) 250, 446, 977
Tru1I TTAA 4 cut(s) 570, 948, 960, 1134
Tru9I TTAA 4 cut(s) 570, 948, 960, 1134
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 7 cut(s) 46, 227, 272, 311, 803, 806, 809
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 315, 534, 655
TspGWI ACGGA 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 1216
XspI CTAG 1 cut(s) 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.