Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008459

Physical Location & Seq

Genomic Coordinates Forward (+)

9810224 .. 9814806

Gene Structure 4583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1111400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGGACGACGATCGAATTCTGGCGCGAGAGAGGCGGCAGATGGAGGAGATCCGACAACTCGATCTCGAAGAACTGCAGGTCGAAGAGGTCGACGAAGATGACTCCTCCGGCGACGACCGCGACGCCACTGGTGGTCGCGGCCCGTCTGATGACTATGCTTTCGATACCTGTTTGGCTTCGTTGCATACGTATCTCGGTGAGGTTGAAGACACTCATCATAGGGTGGCTTTTCTGGATGGGGGCGCCGTCTTGAAGCTCCCTATTTTTTATCTCGAAGGAGTTGTCCTGTTCCCAGAAGCAACCCTTCCCTTAAGAGTCATTCAACCCAGTTTTGTACTTTCTATTGAGAGAGCACTAAACCAAATTGATGCTCCTTGTACCATTGGTGTGATTCATGTATTCAGAGATAATGGAAGAATAAGGTTTGCAAATGTTGGGACAACCGCAGAGATTCGGCAATATAGACGATTAGAGGATGGCTCACTGAATGTGGTAACTCGTGGCCAACAGAGATTTCATCTAAGACACCGTTGGATTGATGCGGAAGGAGCGCCATGTGGGGAAGTTCAAATCATCCAGGAAGTTATACCATCGAGGGTCCCAAGGGATGCATTCGGAGATTTGGCACCATTTAGTAATCCAAGAGGCCACAAATTCTCACTTAAACTGCCTTCAAATGATTCTCGTGCAAAGTCAGTTGGATCTATGGATGTGGACAATGATTCAGAGGCAAAGTCAGATGAAAGCTTTGAGAGTACACTGTCATTCACTGAGAGAGAAATCCACCAATCTGCAATTGGTTCCTATTCTGGATCTGATACAATGGACGAATCAACAACTTCAAGTAGTGATGATGAGAAATCGCAGTTACAATTGCAATCAAAAGACTCTGATTCTTCAGCTTCATTGCATTCATGCCCTGAGATTAGTAATGTTGATTTGGCAAGTAGTTCCATGTCAGACATGCAATCCAGCAAACAGGAAGAGCCAAATAAATGTTGGAGAAATATGGACTTAAATCAGTTTCGTAGAGTTCCAAGAGCATTTTGGCCCCATTGGGTATACCGCATGTATGACTCCTATTGTCTTGCTCAAAGAGCAGCAGAGATGTGGAAACAGATAGTTGGGGCACCAAGCATGGATCGTCTTGTGAAGAAACCTGATCTTTTGTCATTTTTTATCGCTAGTAAAATTCCTGTCTCGGAAGCTACTAGGCAGGAGCTTTTGGAGATTGATGGCATTTCATATAGGCTGCGTAGAGAAATTGAGTTGCTTCACAGTTTTGATCTTATCCGGTGTAAAACATGTCAGACTTTCATTGCAAGGCGGAGTAATATGCTGGTGATGTCAAGTGAAGGTCCTTTTGGTGCTTACGTGAACCCAAGTGGTTGCATTCATGAGATAATGACTCTCTACAAAGCAAATGGCTTGGCACTTATAGGGCCAGCAGTGAGTGAATACAGCTGGTTTCCTGGGTATGCGTGGACAGTAACAAACTGTGCCGGCTGCGAAACCCAAATGGGTTGGCTATTTACAGCCACAAAAAAGAACTTGAAGCCGAAACGTTTTTGGGGAATTCGGAGTTCCCAAGTTTCTGATGACCTGCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002009 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007423 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009887 GO:0009888 GO:0009987 GO:0010498 GO:0016567 GO:0019538 GO:0019941 GO:0030163 GO:0031461 GO:0031464 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0035107 GO:0035113 GO:0035118 GO:0035138 GO:0035239 GO:0035295 GO:0036211 GO:0042471 GO:0042472 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043583 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048729 GO:0048731 GO:0048736 GO:0048839 GO:0048856 GO:0051603 GO:0060173 GO:0060429 GO:0060562 GO:0070647 GO:0071599 GO:0071600 GO:0071704 GO:0080008 GO:0090596 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LON_substr_bdg Yippee-Mis18

Protein Analysis

537

Amino Acids

60.53

Weight (kDa)

5.18

Isoelectric Point (pI)

53.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	85 - 426	2.6e-28	ATP-dependent protease La (LON) substrate-binding domain
Yippee-Mis18	PF03226	431 - 531	2e-07	Yippee zinc-binding/DNA-binding /Mis18, centromere assembly

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25740 AT2G25740
fragaria_vesca	FvH4_2g35320 FvH4_2g35320 FvH4_2g35320 FvH4_2g35320
malus_domestica	MD08G1111400.v1.1 MD15G1090900.v1.1
prunus_persica	Prupe.1G445300_v2.0.a1 Prupe.1G445300_v2.0.a1
pyrus_communis	pycom08g09250 pycom15g08480 pycom15g08490
rosa_chinensis	RchiOBHm_Chr6g0306601
rosa_laevigata	RLG00000010781
rosa_multiflora	Rmu_sc0017624.1_g000002
rosa_roxburghii	Rroxscaffold_7G00161620
rosa_rugosa	Rorug06G0350900
rosa_samantha	Rh6AG463200 Rh6BG437700 Rh6CG477500 Rh6DG464000
rosa_wichuraiana	Rw6G040360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	67
AccB1I	GGYRCC	3 cut(s)	242, 625, 1129
AccI	GTMKAC	2 cut(s)	90, 1062
AccII	CGCG	3 cut(s)	25, 120, 138
AciI	CCGC	7 cut(s)	34, 118, 138, 444, 542, 1066, 1327
AclI	AACGTT	1 cut(s)	1564
AclWI	GGATC	4 cut(s)	43, 709, 820, 1149
AcoI	YGGCCR	1 cut(s)	502
AcsI	RAATTY	4 cut(s)	15, 653, 1191, 1575
AcuI	CTGAAG	1 cut(s)	882
AcyI	GRCGYC	2 cut(s)	123, 243
AfaI	GTAC	3 cut(s)	336, 379, 757
AflII	CTTAAG	1 cut(s)	310
AflIII	ACRYGT	1 cut(s)	1304
AgsI	TTSAA	7 cut(s)	206, 253, 323, 569, 675, 843, 1555
AjnI	CCWGG	2 cut(s)	576, 1471
AjuI	GAANNNNNNNTTGG	4 cut(s)	498, 530, 1347, 1379
AluBI	AGCT	6 cut(s)	256, 747, 902, 1208, 1222, 1464
AluI	AGCT	6 cut(s)	256, 747, 902, 1208, 1222, 1464
Alw21I	GWGCWC	1 cut(s)	355
Alw26I	GTCTC	1 cut(s)	1204
AlwI	GGATC	4 cut(s)	43, 709, 820, 1149
AoxI	GGCC	5 cut(s)	139, 502, 646, 1049, 1442
ApeKI	GCWGC	3 cut(s)	1100, 1252, 1506
ApoI	RAATTY	4 cut(s)	15, 653, 1191, 1575
ArsI	GACNNNNNNTTYG	4 cut(s)	143, 175, 267, 299
AspLEI	GCGC	3 cut(s)	25, 245, 553
AspS9I	GGNCC	5 cut(s)	140, 598, 1050, 1358, 1442
AsuHPI	GGTGA	2 cut(s)	209, 1354
AvaII	GGWCC	2 cut(s)	598, 1358
BaeGI	GKGCMC	1 cut(s)	1132
BalI	TGGCCA	1 cut(s)	504
BanI	GGYRCC	3 cut(s)	242, 625, 1129
BauI	CACGAG	2 cut(s)	498, 684
BbsI	GAAGAC	1 cut(s)	213
Bbv12I	GWGCWC	1 cut(s)	355
BbvI	GCAGC	3 cut(s)	1112, 1239, 1493
BccI	CCATC	5 cut(s)	34, 230, 470, 598, 1229
BceAI	ACGGC	1 cut(s)	230
BciT130I	CCWGG	2 cut(s)	578, 1473
BcoDI	GTCTC	1 cut(s)	1204
BfaI	CTAG	3 cut(s)	1185, 1212, 1609
BfmI	CTRYAG	1 cut(s)	74
BfoI	RGCGCY	2 cut(s)	246, 554
BfrI	CTTAAG	1 cut(s)	310
BfuAI	ACCTGC	1 cut(s)	67
BisI	GCNGC	5 cut(s)	35, 139, 1101, 1253, 1507
BlsI	GCNGC	5 cut(s)	36, 140, 1102, 1254, 1508
Bme1390I	CCNGG	2 cut(s)	578, 1473
Bme18I	GGWCC	2 cut(s)	598, 1358
BmgT120I	GGNCC	5 cut(s)	140, 598, 1050, 1358, 1442
BmiI	GGNNCC	7 cut(s)	244, 599, 600, 627, 802, 1052, 1131
BmrFI	CCNGG	2 cut(s)	578, 1473
BmrI	ACTGGG	1 cut(s)	321
BmsI	GCATC	3 cut(s)	358, 529, 598
BmuI	ACTGGG	1 cut(s)	321
BpiI	GAAGAC	1 cut(s)	213
BplI	GAGNNNNNCTC	2 cut(s)	464, 496
BsaAI	YACGTR	2 cut(s)	189, 1375
BsaHI	GRCGYC	2 cut(s)	123, 243
BsaJI	CCNNGG	2 cut(s)	602, 1472
BsaWI	WCCGGW	1 cut(s)	1293
Bse118I	RCCGGY	1 cut(s)	1502
Bse1I	ACTGG	2 cut(s)	133, 327
Bse3DI	GCAATG	2 cut(s)	905, 1317
BseBI	CCWGG	2 cut(s)	578, 1473
BseDI	CCNNGG	2 cut(s)	602, 1472
BseGI	GGATG	5 cut(s)	241, 481, 573, 613, 715
BseMI	GCAATG	2 cut(s)	905, 1317
BseMII	CTCAG	2 cut(s)	762, 912
BseNI	ACTGG	2 cut(s)	133, 327
BseRI	GAGGAG	2 cut(s)	59, 94
BseSI	GKGCMC	1 cut(s)	1132
BseXI	GCAGC	3 cut(s)	1112, 1239, 1493
BsgI	GTGCAG	1 cut(s)	1589
Bsh1236I	CGCG	3 cut(s)	25, 120, 138
Bsh1285I	CGRYCG	2 cut(s)	13, 118
BshFI	GGCC	5 cut(s)	141, 504, 648, 1051, 1444
BshNI	GGYRCC	3 cut(s)	242, 625, 1129
BsiEI	CGRYCG	2 cut(s)	13, 118
BsiHKAI	GWGCWC	1 cut(s)	355
BsiSI	CCGG	3 cut(s)	108, 1294, 1503
BslFI	GGGAC	2 cut(s)	451, 584
BsmAI	GTCTC	1 cut(s)	1204
BsmFI	GGGAC	2 cut(s)	451, 584
BsmI	GAATGC	3 cut(s)	611, 910, 1392
BsnI	GGCC	5 cut(s)	141, 504, 648, 1051, 1444
Bsp1286I	GDGCHC	2 cut(s)	355, 1132
Bsp143I	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
BspACI	CCGC	7 cut(s)	34, 118, 138, 444, 542, 1066, 1327
BspANI	GGCC	5 cut(s)	141, 504, 648, 1051, 1444
BspCNI	CTCAG	2 cut(s)	763, 913
BspFNI	CGCG	3 cut(s)	25, 120, 138
BspHI	TCATGA	1 cut(s)	1396
BspLI	GGNNCC	7 cut(s)	244, 599, 600, 627, 802, 1052, 1131
BspMAI	CTGCAG	1 cut(s)	78
BspMI	ACCTGC	1 cut(s)	67
BspPI	GGATC	4 cut(s)	43, 709, 820, 1149
BspQI	GCTCTTC	1 cut(s)	978
BspT107I	GGYRCC	3 cut(s)	242, 625, 1129
BspTI	CTTAAG	1 cut(s)	310
BsrDI	GCAATG	2 cut(s)	905, 1317
BsrFI	RCCGGY	1 cut(s)	1502
BsrI	ACTGG	2 cut(s)	133, 327
BssAI	RCCGGY	1 cut(s)	1502
BssECI	CCNNGG	2 cut(s)	602, 1472
BssMI	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
BssNAI	GTATAC	1 cut(s)	1063
BssNI	GRCGYC	2 cut(s)	123, 243
BssSI	CACGAG	2 cut(s)	498, 684
BssT1I	CCWWGG	1 cut(s)	602
Bst1107I	GTATAC	1 cut(s)	1063
Bst2BI	CACGAG	2 cut(s)	498, 684
Bst2UI	CCWGG	2 cut(s)	578, 1473
Bst4CI	ACNGT	5 cut(s)	530, 762, 1280, 1489, 1499
Bst6I	CTCTTC	2 cut(s)	78, 978
BstACI	GRCGYC	2 cut(s)	123, 243
BstAFI	CTTAAG	1 cut(s)	310
BstBAI	YACGTR	2 cut(s)	189, 1375
BstC8I	GCNNGC	2 cut(s)	1446, 1504
BstDEI	CTNAG	3 cut(s)	521, 771, 921
BstF5I	GGATG	5 cut(s)	241, 481, 573, 613, 715
BstFNI	CGCG	3 cut(s)	25, 120, 138
BstH2I	RGCGCY	2 cut(s)	246, 554
BstHHI	GCGC	3 cut(s)	25, 245, 553
BstKTI	GATC	8 cut(s)	13, 51, 64, 704, 815, 1144, 1165, 1288
BstMAI	GTCTC	1 cut(s)	1204
BstMBI	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
BstMCI	CGRYCG	2 cut(s)	13, 118
BstMWI	GCNNNNNNNGC	4 cut(s)	31, 117, 548, 1097
BstNI	CCWGG	2 cut(s)	578, 1473
BstNSI	RCATGY	3 cut(s)	967, 1072, 1308
BstSCI	CCNGG	2 cut(s)	576, 1471
BstSFI	CTRYAG	1 cut(s)	74
BstSLI	GKGCMC	1 cut(s)	1132
BstSNI	TACGTA	1 cut(s)	189
BstUI	CGCG	3 cut(s)	25, 120, 138
BstV1I	GCAGC	3 cut(s)	1112, 1239, 1493
BstV2I	GAAGAC	1 cut(s)	213
BstX2I	RGATCY	3 cut(s)	48, 701, 812
BstYI	RGATCY	3 cut(s)	48, 701, 812
BstZ17I	GTATAC	1 cut(s)	1063
BsuRI	GGCC	5 cut(s)	141, 504, 648, 1051, 1444
BtsCI	GGATG	5 cut(s)	241, 481, 573, 613, 715
BtsI	GCAGTG	1 cut(s)	1455
BtsIMutI	CAGTG	5 cut(s)	126, 482, 758, 768, 1455
BveI	ACCTGC	1 cut(s)	67
Cac8I	GCNNGC	2 cut(s)	1446, 1504
CciI	TCATGA	1 cut(s)	1396
CfoI	GCGC	3 cut(s)	25, 245, 553
Cfr10I	RCCGGY	1 cut(s)	1502
Cfr13I	GGNCC	5 cut(s)	140, 598, 1050, 1358, 1442
CseI	GACGC	1 cut(s)	131
Csp6I	GTAC	3 cut(s)	335, 378, 756
CviAII	CATG	9 cut(s)	395, 555, 915, 955, 964, 1069, 1138, 1305, 1397
CviQI	GTAC	3 cut(s)	335, 378, 756
DdeI	CTNAG	3 cut(s)	521, 771, 921
DinI	GGCGCC	1 cut(s)	244
DpnI	GATC	8 cut(s)	12, 50, 63, 703, 814, 1143, 1164, 1287
DpnII	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
EaeI	YGGCCR	1 cut(s)	502
Eam1104I	CTCTTC	2 cut(s)	78, 978
EarI	CTCTTC	2 cut(s)	78, 978
EciI	GGCGGA	1 cut(s)	1342
Eco105I	TACGTA	1 cut(s)	189
Eco130I	CCWWGG	1 cut(s)	602
Eco47I	GGWCC	2 cut(s)	598, 1358
Eco57I	CTGAAG	1 cut(s)	882
EcoO109I	RGGNCCY	2 cut(s)	598, 1358
EcoRI	GAATTC	2 cut(s)	15, 1575
EcoRII	CCWGG	2 cut(s)	576, 1471
EcoT14I	CCWWGG	1 cut(s)	602
EcoT22I	ATGCAT	1 cut(s)	613
EgeI	GGCGCC	1 cut(s)	244
EheI	GGCGCC	1 cut(s)	244
ErhI	CCWWGG	1 cut(s)	602
FaeI	CATG	9 cut(s)	398, 558, 918, 958, 967, 1072, 1141, 1308, 1400
FalI	AAGNNNNNCTT	2 cut(s)	288, 320
FaqI	GGGAC	2 cut(s)	451, 584
FatI	CATG	9 cut(s)	394, 554, 914, 954, 963, 1068, 1137, 1304, 1396
FblI	GTMKAC	2 cut(s)	90, 1062
Fnu4HI	GCNGC	5 cut(s)	35, 139, 1101, 1253, 1507
FokI	GGATG	5 cut(s)	248, 488, 560, 620, 722
Fsp4HI	GCNGC	5 cut(s)	35, 139, 1101, 1253, 1507
FspBI	CTAG	3 cut(s)	1185, 1212, 1609
GlaI	GCGC	3 cut(s)	24, 244, 552
GluI	GCNGC	5 cut(s)	35, 139, 1101, 1253, 1507
HaeII	RGCGCY	2 cut(s)	246, 554
HaeIII	GGCC	5 cut(s)	141, 504, 648, 1051, 1444
HapII	CCGG	3 cut(s)	108, 1294, 1503
HgaI	GACGC	1 cut(s)	131
HhaI	GCGC	3 cut(s)	25, 245, 553
Hin1I	GRCGYC	2 cut(s)	123, 243
Hin1II	CATG	9 cut(s)	398, 558, 918, 958, 967, 1072, 1141, 1308, 1400
Hin6I	GCGC	3 cut(s)	23, 243, 551
HinP1I	GCGC	3 cut(s)	23, 243, 551
HincII	GTYRAC	1 cut(s)	91
HindII	GTYRAC	1 cut(s)	91
HindIII	AAGCTT	1 cut(s)	745
HpaII	CCGG	3 cut(s)	108, 1294, 1503
HphI	GGTGA	2 cut(s)	209, 1354
Hpy166II	GTNNAC	6 cut(s)	91, 715, 758, 1063, 1378, 1485
Hpy188III	TCNNGA	6 cut(s)	65, 233, 250, 272, 810, 1397
Hpy8I	GTNNAC	6 cut(s)	91, 715, 758, 1063, 1378, 1485
Hpy99I	CGWCG	4 cut(s)	11, 95, 116, 125
HpyAV	CCTTC	5 cut(s)	269, 314, 539, 681, 1349
HpyCH4III	ACNGT	5 cut(s)	530, 762, 1280, 1489, 1499
HpyCH4IV	ACGT	3 cut(s)	188, 1374, 1564
HpyF10VI	GCNNNNNNNGC	4 cut(s)	31, 117, 548, 1097
HpyF3I	CTNAG	3 cut(s)	521, 771, 921
HpySE526I	ACGT	3 cut(s)	188, 1374, 1564
Hsp92I	GRCGYC	2 cut(s)	123, 243
Hsp92II	CATG	9 cut(s)	398, 558, 918, 958, 967, 1072, 1141, 1308, 1400
HspAI	GCGC	3 cut(s)	23, 243, 551
KasI	GGCGCC	1 cut(s)	242
KflI	GGGWCCC	1 cut(s)	598
KroI	GCCGGC	1 cut(s)	1502
KroNI	GCCGGC	1 cut(s)	1504
Kzo9I	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
LguI	GCTCTTC	1 cut(s)	978
LmnI	GCTCC	4 cut(s)	261, 376, 548, 1219
Lsp1109I	GCAGC	3 cut(s)	1112, 1239, 1493
LweI	GCATC	3 cut(s)	358, 529, 598
MaeI	CTAG	3 cut(s)	1185, 1212, 1609
MaeII	ACGT	3 cut(s)	188, 1374, 1564
MaeIII	GTNAC	3 cut(s)	493, 867, 1489
MalI	GATC	8 cut(s)	12, 50, 63, 703, 814, 1143, 1164, 1287
MboI	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
MboII	GAAGA	8 cut(s)	80, 95, 107, 218, 426, 888, 995, 1165
MfeI	CAATTG	2 cut(s)	795, 872
MflI	RGATCY	3 cut(s)	48, 701, 812
MhlI	GDGCHC	2 cut(s)	355, 1132
MlsI	TGGCCA	1 cut(s)	504
MluCI	AATT	8 cut(s)	15, 363, 653, 795, 872, 1191, 1263, 1575
MluNI	TGGCCA	1 cut(s)	504
Mly113I	GGCGCC	1 cut(s)	243
MlyI	GAGTC	5 cut(s)	95, 324, 881, 1070, 1402
MmeI	TCCRAC	4 cut(s)	76, 512, 679, 980
MnlI	CCTC	9 cut(s)	24, 37, 79, 115, 193, 466, 588, 638, 721
Mox20I	TGGCCA	1 cut(s)	504
Mph1103I	ATGCAT	1 cut(s)	613
MroNI	GCCGGC	1 cut(s)	1502
MscI	TGGCCA	1 cut(s)	504
MseI	TTAA	3 cut(s)	311, 663, 1016
MslI	CAYNNNNRTG	1 cut(s)	386
Msp20I	TGGCCA	1 cut(s)	504
MspA1I	CMGCKG	1 cut(s)	1464
MspCI	CTTAAG	1 cut(s)	310
MspI	CCGG	3 cut(s)	108, 1294, 1503
MspR9I	CCNGG	2 cut(s)	578, 1473
MunI	CAATTG	2 cut(s)	795, 872
Mva1269I	GAATGC	3 cut(s)	611, 910, 1392
MvaI	CCWGG	2 cut(s)	578, 1473
MvnI	CGCG	3 cut(s)	25, 120, 138
MwoI	GCNNNNNNNGC	4 cut(s)	31, 117, 548, 1097
NaeI	GCCGGC	1 cut(s)	1504
NarI	GGCGCC	1 cut(s)	243
NdeII	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
NgoMIV	GCCGGC	1 cut(s)	1502
NlaIII	CATG	9 cut(s)	398, 558, 918, 958, 967, 1072, 1141, 1308, 1400
NlaIV	GGNNCC	7 cut(s)	244, 599, 600, 627, 802, 1052, 1131
NsiI	ATGCAT	1 cut(s)	613
NspI	RCATGY	3 cut(s)	967, 1072, 1308
PagI	TCATGA	1 cut(s)	1396
PciI	ACATGT	1 cut(s)	1304
PciSI	GCTCTTC	1 cut(s)	978
PcsI	WCGNNNNNNNCGW	2 cut(s)	87, 185
PctI	GAATGC	3 cut(s)	611, 910, 1392
PdiI	GCCGGC	1 cut(s)	1504
PfeI	GAWTC	6 cut(s)	391, 451, 680, 722, 830, 893
PfoI	TCCNGGA	1 cut(s)	576
PkrI	GCNGC	5 cut(s)	36, 140, 1102, 1254, 1508
Ple19I	CGATCG	1 cut(s)	13
PleI	GAGTC	5 cut(s)	95, 323, 881, 1070, 1402
PluTI	GGCGCC	1 cut(s)	246
PpsI	GAGTC	5 cut(s)	95, 323, 881, 1070, 1402
Ppu21I	YACGTR	2 cut(s)	189, 1375
PpuMI	RGGWCCY	2 cut(s)	598, 1358
PscI	ACATGT	1 cut(s)	1304
Psp1406I	AACGTT	1 cut(s)	1564
Psp5II	RGGWCCY	2 cut(s)	598, 1358
Psp6I	CCWGG	2 cut(s)	576, 1471
PspGI	CCWGG	2 cut(s)	576, 1471
PspN4I	GGNNCC	7 cut(s)	244, 599, 600, 627, 802, 1052, 1131
PspPI	GGNCC	5 cut(s)	140, 598, 1050, 1358, 1442
PspPPI	RGGWCCY	2 cut(s)	598, 1358
PstI	CTGCAG	1 cut(s)	78
PsuI	RGATCY	3 cut(s)	48, 701, 812
PvuI	CGATCG	1 cut(s)	13
PvuII	CAGCTG	1 cut(s)	1464
RsaI	GTAC	3 cut(s)	336, 379, 757
RsaNI	GTAC	3 cut(s)	335, 378, 756
RseI	CAYNNNNRTG	1 cut(s)	386
SalI	GTCGAC	1 cut(s)	89
SapI	GCTCTTC	1 cut(s)	978
SaqAI	TTAA	3 cut(s)	311, 663, 1016
SatI	GCNGC	5 cut(s)	35, 139, 1101, 1253, 1507
Sau3AI	GATC	8 cut(s)	10, 48, 61, 701, 812, 1141, 1162, 1285
Sau96I	GGNCC	5 cut(s)	140, 598, 1050, 1358, 1442
SchI	GAGTC	5 cut(s)	95, 324, 881, 1070, 1402
ScrFI	CCNGG	2 cut(s)	578, 1473
SduI	GDGCHC	2 cut(s)	355, 1132
SfaNI	GCATC	3 cut(s)	358, 529, 598
SfcI	CTRYAG	1 cut(s)	74
SfoI	GGCGCC	1 cut(s)	244
SinI	GGWCC	2 cut(s)	598, 1358
SmiMI	CAYNNNNRTG	1 cut(s)	386
SmlI	CTYRAG	1 cut(s)	310
SmoI	CTYRAG	1 cut(s)	310
SnaBI	TACGTA	1 cut(s)	189
Sse9I	AATT	8 cut(s)	15, 363, 653, 795, 872, 1191, 1263, 1575
SsiI	CCGC	7 cut(s)	34, 118, 138, 444, 542, 1066, 1327
SspDI	GGCGCC	1 cut(s)	242
SspMI	CTAG	3 cut(s)	1185, 1212, 1609
StyD4I	CCNGG	2 cut(s)	576, 1471
StyI	CCWWGG	1 cut(s)	602
TaaI	ACNGT	5 cut(s)	530, 762, 1280, 1489, 1499
TaiI	ACGT	3 cut(s)	191, 1377, 1567
TaqI	TCGA	8 cut(s)	13, 60, 66, 81, 90, 162, 273, 593
TasI	AATT	8 cut(s)	15, 363, 653, 795, 872, 1191, 1263, 1575
TatI	WGTACW	2 cut(s)	334, 755
TauI	GCSGC	2 cut(s)	37, 141
TfiI	GAWTC	6 cut(s)	391, 451, 680, 722, 830, 893
Tru1I	TTAA	3 cut(s)	311, 663, 1016
Tru9I	TTAA	3 cut(s)	311, 663, 1016
TscAI	CASTG	5 cut(s)	133, 489, 765, 775, 1455
TseI	GCWGC	3 cut(s)	1100, 1252, 1506
TspDTI	ATGAA	8 cut(s)	383, 506, 756, 894, 903, 1233, 1306, 1385
TspRI	CASTG	5 cut(s)	133, 489, 765, 775, 1455
Vha464I	CTTAAG	1 cut(s)	310
VpaK11BI	GGWCC	2 cut(s)	598, 1358
XapI	RAATTY	4 cut(s)	15, 653, 1191, 1575
XceI	RCATGY	3 cut(s)	967, 1072, 1308
XcmI	CCANNNNNNNNNTGG	3 cut(s)	794, 996, 1044
XmiI	GTMKAC	2 cut(s)	90, 1062
XspI	CTAG	3 cut(s)	1185, 1212, 1609
Zsp2I	ATGCAT	1 cut(s)	613

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.