Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0008459

Physical Location & Seq

Genomic Coordinates Reverse (-)

64571185 .. 64575075

Gene Structure 3891 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG477500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGGCTGATGAATATACCTTCAATACTTGTTTGGCTTCATTGCATACGTATCTTGGCGAGGTTGAAGACACTCATCATAGGATGGCGTTTTTGGATGGCGGTGCGGTATTGAACCTCCCAATCTTCTATCTCGAAGGAATTGTCCTGTTCCCAGACGCTACCCTTCCACTAAGAGTTATTCAACCCAATTTTGTAGTCGCTATTGAGAGAGCACTATCCCAAGTTGACGCTCCTTATACCATTGGTGTGATTCGTGTTTACAGAGATCCCAACAATAGAAGCATAAAGTTTGCAAATATTGGGACAACTGCGGAGATTCGCCAATATAGGAGATTAGAGGATGGCTCACTGAACGTGGTAACCCGTGGTCAACAGCGATTTCGTCTTAGACGCCGTTGGATTGATGTGGAAGGAGCGCCATACGGGGAAATCCAAATTATAGAAGAAGATGTACCATTGAGGGCTCCACGGGCTGCATTTGGAGATCTGGCACCATTTAGTAATCGAAAAGGCCATAGTTTCCCGCGTGCAATGCCTTCGCACGCTTCTCGTGGAAATTCAAATGGATCTATGGATGTGGACAATGACTCAGAGGCAAATTCAGATGAAAGCTTTGAGAGTGCACTTTCTTTGACAGAAAAGGGAATTCACCAATCTGCAATTGAATCCTATTATGGATCTGATGTGTCAACAAGCAGTGATGGTGAGAAGTCAGAATTCTTATCTCAAGGATCACAATCAAAAGACTCTGATTCCACAGATTCATTGCATTCGTGCCCTGAGAGTTTGGTTGATTTGGGGAGCAGTTCCATATCGGAGATGCAATCATCCAAACAGAAAGATCCAAGTAGTTGGAGAAATACAGACTCTAAACCGATACGTAGAGTTCCAAGAGCATTCTGGCCCTATTGGGTATATCGCATGTATGACTCCTATTGTCTTGCTCAAGGAGTGGCAGATATGTGGAAACAGATAGTTGGGGCACCATTAATGGATGGTCTGGTGAAGAAGCCTGATCTTTTGTCATTTTACATCGCTAGTAAACTTCCTGTTTTTGAATCTACAAGACAGGAGCTTTTGGAGATTGATGGTATTTCATATAGGTTACGTCGGGAAATTGAGTTGCTTCAGACTGTTAATCTTCTCCGGTGTAAAACTTGTCAGACTGTTTTTGCAAGGCGGAGTGATATGCTAGTGATGTCAAGTGATGGTCCTCTTGGTGCTTATGTAAACCCCTATGGTTGTGTACACGAGATAATGACTCTCTACAAAGCAACTGGCTTGGCACTCGTAGGTTCAGCAGTGAAAGAATACAGCTGGTTTCCTGGGTATGCATGGACAGTAACAAACTGTGCCACCTGTGAAACTCAAATGGGTTGGCTCTTCACAGCCACAAAAAAGAATTTGAAGCCTAAATTGTTTTGGGGGATTCGGAGTTCCCAAGTTTCTGATGACTTGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002009 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007423 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009887 GO:0009888 GO:0009987 GO:0010498 GO:0016567 GO:0019538 GO:0019941 GO:0030163 GO:0031461 GO:0031464 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033333 GO:0033334 GO:0033339 GO:0035107 GO:0035113 GO:0035118 GO:0035138 GO:0035239 GO:0035295 GO:0036211 GO:0042471 GO:0042472 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043583 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048513 GO:0048562 GO:0048568 GO:0048598 GO:0048729 GO:0048731 GO:0048736 GO:0048839 GO:0048856 GO:0051603 GO:0060173 GO:0060429 GO:0060562 GO:0070647 GO:0071599 GO:0071600 GO:0071704 GO:0080008 GO:0090596 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LON_substr_bdg Yippee-Mis18

Protein Analysis

487

Amino Acids

54.67

Weight (kDa)

5.81

Isoelectric Point (pI)

49.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	38 - 162	2.5e-22	ATP-dependent protease La (LON) substrate-binding domain
Yippee-Mis18	PF03226	382 - 481	1.2e-07	Yippee zinc-binding/DNA-binding /Mis18, centromere assembly

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25740 AT2G25740
fragaria_vesca	FvH4_2g35320 FvH4_2g35320 FvH4_2g35320 FvH4_2g35320
malus_domestica	MD08G1111400.v1.1 MD15G1090900.v1.1
prunus_persica	Prupe.1G445300_v2.0.a1 Prupe.1G445300_v2.0.a1
pyrus_communis	pycom08g09250 pycom15g08480 pycom15g08490
rosa_chinensis	RchiOBHm_Chr6g0306601
rosa_laevigata	RLG00000010781
rosa_multiflora	Rmu_sc0017624.1_g000002
rosa_roxburghii	Rroxscaffold_7G00161620
rosa_rugosa	Rorug06G0350900
rosa_samantha	Rh6AG463200 Rh6BG437700 Rh6CG477500 Rh6DG464000
rosa_wichuraiana	Rw6G040360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	490, 982
AccII	CGCG	1 cut(s)	526
AciI	CCGC	5 cut(s)	99, 104, 311, 524, 1180
AclWI	GGATC	5 cut(s)	260, 574, 685, 739, 836
AcsI	RAATTY	5 cut(s)	556, 598, 645, 716, 1402
AcuI	CTGAAG	1 cut(s)	1112
AcyI	GRCGYC	1 cut(s)	391
AfaI	GTAC	2 cut(s)	453, 1248
AgsI	TTSAA	8 cut(s)	22, 65, 112, 182, 561, 665, 1058, 1408
AjnI	CCWGG	1 cut(s)	1324
AloI	GAACNNNNNNTCC	2 cut(s)	1420, 1452
AluBI	AGCT	3 cut(s)	612, 1075, 1317
AluI	AGCT	3 cut(s)	612, 1075, 1317
Alw21I	GWGCWC	2 cut(s)	214, 625
Alw44I	GTGCAC	1 cut(s)	621
AlwI	GGATC	5 cut(s)	260, 574, 685, 739, 836
AoxI	GGCC	2 cut(s)	511, 902
ApaLI	GTGCAC	1 cut(s)	621
ApeKI	GCWGC	1 cut(s)	473
ApoI	RAATTY	5 cut(s)	556, 598, 645, 716, 1402
ArsI	GACNNNNNNTTYG	2 cut(s)	126, 158
AseI	ATTAAT	1 cut(s)	989
AspLEI	GCGC	1 cut(s)	418
AspS9I	GGNCC	2 cut(s)	903, 1211
AsuHPI	GGTGA	3 cut(s)	641, 716, 1015
AvaII	GGWCC	1 cut(s)	1211
BaeGI	GKGCMC	3 cut(s)	625, 779, 985
BanI	GGYRCC	2 cut(s)	490, 982
BanII	GRGCYC	1 cut(s)	466
BarI	GAAGNNNNNNTAC	2 cut(s)	435, 467
BauI	CACGAG	2 cut(s)	549, 1250
BbsI	GAAGAC	1 cut(s)	72
Bbv12I	GWGCWC	2 cut(s)	214, 625
BbvI	GCAGC	1 cut(s)	460
BccI	CCATC	7 cut(s)	76, 89, 335, 695, 989, 1082, 1202
BceAI	ACGGC	1 cut(s)	378
BcgI	CGANNNNNNTGC	4 cut(s)	519, 530, 553, 564
BciT130I	CCWGG	1 cut(s)	1326
BfaI	CTAG	2 cut(s)	1038, 1193
BfoI	RGCGCY	1 cut(s)	419
BglII	AGATCT	1 cut(s)	484
BisI	GCNGC	1 cut(s)	474
BlsI	GCNGC	1 cut(s)	475
Bme1390I	CCNGG	1 cut(s)	1326
Bme18I	GGWCC	1 cut(s)	1211
BmgT120I	GGNCC	2 cut(s)	903, 1211
BmiI	GGNNCC	3 cut(s)	465, 492, 984
BmrFI	CCNGG	1 cut(s)	1326
BmsI	GCATC	1 cut(s)	810
BpiI	GAAGAC	1 cut(s)	72
BplI	GAGNNNNNCTC	2 cut(s)	329, 361
BpuEI	CTTGAG	2 cut(s)	711, 930
BsaAI	YACGTR	2 cut(s)	48, 881
BsaBI	GATNNNNATC	1 cut(s)	736
BsaHI	GRCGYC	1 cut(s)	391
BsaJI	CCNNGG	3 cut(s)	364, 467, 1325
BsaWI	WCCGGW	1 cut(s)	1146
Bse1I	ACTGG	1 cut(s)	1282
Bse3DI	GCAATG	3 cut(s)	38, 537, 764
Bse8I	GATNNNNATC	1 cut(s)	736
BseBI	CCWGG	1 cut(s)	1326
BseDI	CCNNGG	3 cut(s)	364, 467, 1325
BseGI	GGATG	6 cut(s)	87, 100, 346, 580, 827, 1000
BseJI	GATNNNNATC	1 cut(s)	736
BseMI	GCAATG	3 cut(s)	38, 537, 764
BseMII	CTCAG	2 cut(s)	603, 771
BseNI	ACTGG	1 cut(s)	1282
BseSI	GKGCMC	3 cut(s)	625, 779, 985
BseXI	GCAGC	1 cut(s)	460
Bsh1236I	CGCG	1 cut(s)	526
BshFI	GGCC	2 cut(s)	513, 904
BshNI	GGYRCC	2 cut(s)	490, 982
BsiHKAI	GWGCWC	2 cut(s)	214, 625
BsiSI	CCGG	1 cut(s)	1147
BslFI	GGGAC	1 cut(s)	316
BsmFI	GGGAC	1 cut(s)	316
BsmI	GAATGC	2 cut(s)	769, 896
BsnI	GGCC	2 cut(s)	513, 904
Bsp1286I	GDGCHC	5 cut(s)	214, 466, 625, 779, 985
Bsp1407I	TGTACA	1 cut(s)	1246
Bsp143I	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
BspACI	CCGC	5 cut(s)	99, 104, 311, 524, 1180
BspANI	GGCC	2 cut(s)	513, 904
BspCNI	CTCAG	2 cut(s)	602, 772
BspFNI	CGCG	1 cut(s)	526
BspLI	GGNNCC	3 cut(s)	465, 492, 984
BspPI	GGATC	5 cut(s)	260, 574, 685, 739, 836
BspQI	GCTCTTC	1 cut(s)	1388
BspT107I	GGYRCC	2 cut(s)	490, 982
BsrDI	GCAATG	3 cut(s)	38, 537, 764
BsrGI	TGTACA	1 cut(s)	1246
BsrI	ACTGG	1 cut(s)	1282
BssECI	CCNNGG	3 cut(s)	364, 467, 1325
BssMI	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
BssNI	GRCGYC	1 cut(s)	391
BssSI	CACGAG	2 cut(s)	549, 1250
Bst2BI	CACGAG	2 cut(s)	549, 1250
Bst2UI	CCWGG	1 cut(s)	1326
Bst4CI	ACNGT	4 cut(s)	1135, 1168, 1342, 1352
Bst6I	CTCTTC	1 cut(s)	1388
BstACI	GRCGYC	1 cut(s)	391
BstAUI	TGTACA	1 cut(s)	1246
BstBAI	YACGTR	2 cut(s)	48, 881
BstC8I	GCNNGC	2 cut(s)	528, 543
BstDEI	CTNAG	4 cut(s)	170, 386, 589, 780
BstDSI	CCRYGG	2 cut(s)	364, 467
BstEII	GGTNACC	1 cut(s)	358
BstF5I	GGATG	6 cut(s)	87, 100, 346, 580, 827, 1000
BstFNI	CGCG	1 cut(s)	526
BstH2I	RGCGCY	1 cut(s)	419
BstHHI	GCGC	1 cut(s)	418
BstKTI	GATC	7 cut(s)	268, 487, 569, 680, 734, 844, 1018
BstMBI	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
BstMWI	GCNNNNNNNGC	2 cut(s)	470, 532
BstNI	CCWGG	1 cut(s)	1326
BstNSI	RCATGY	1 cut(s)	925
BstPI	GGTNACC	1 cut(s)	358
BstSCI	CCNGG	1 cut(s)	1324
BstSLI	GKGCMC	3 cut(s)	625, 779, 985
BstSNI	TACGTA	2 cut(s)	48, 881
BstUI	CGCG	1 cut(s)	526
BstV1I	GCAGC	1 cut(s)	460
BstV2I	GAAGAC	1 cut(s)	72
BstX2I	RGATCY	5 cut(s)	265, 484, 566, 677, 841
BstXI	CCANNNNNNTGG	1 cut(s)	852
BstYI	RGATCY	5 cut(s)	265, 484, 566, 677, 841
BsuRI	GGCC	2 cut(s)	513, 904
BtgI	CCRYGG	2 cut(s)	364, 467
BtgZI	GCGATG	1 cut(s)	1018
BtsCI	GGATG	6 cut(s)	87, 100, 346, 580, 827, 1000
BtsI	GCAGTG	2 cut(s)	703, 1308
BtsIMutI	CAGTG	3 cut(s)	347, 703, 1308
Cac8I	GCNNGC	2 cut(s)	528, 543
CfoI	GCGC	1 cut(s)	418
Cfr13I	GGNCC	2 cut(s)	903, 1211
CseI	GACGC	3 cut(s)	164, 236, 399
Csp6I	GTAC	2 cut(s)	452, 1247
CviAII	CATG	2 cut(s)	922, 1335
CviQI	GTAC	2 cut(s)	452, 1247
DdeI	CTNAG	4 cut(s)	170, 386, 589, 780
DpnI	GATC	7 cut(s)	267, 486, 568, 679, 733, 843, 1017
DpnII	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
Eam1104I	CTCTTC	1 cut(s)	1388
EarI	CTCTTC	1 cut(s)	1388
EciI	GGCGGA	1 cut(s)	1195
Eco105I	TACGTA	2 cut(s)	48, 881
Eco24I	GRGCYC	1 cut(s)	466
Eco47I	GGWCC	1 cut(s)	1211
Eco57I	CTGAAG	1 cut(s)	1112
Eco91I	GGTNACC	1 cut(s)	358
EcoO65I	GGTNACC	1 cut(s)	358
EcoRI	GAATTC	2 cut(s)	645, 716
EcoRII	CCWGG	1 cut(s)	1324
EcoT22I	ATGCAT	1 cut(s)	1336
EcoT38I	GRGCYC	1 cut(s)	466
FaeI	CATG	2 cut(s)	925, 1338
FaqI	GGGAC	1 cut(s)	316
FatI	CATG	2 cut(s)	921, 1334
FauI	CCCGC	1 cut(s)	531
Fnu4HI	GCNGC	1 cut(s)	474
FokI	GGATG	6 cut(s)	94, 107, 353, 587, 814, 1007
FriOI	GRGCYC	1 cut(s)	466
Fsp4HI	GCNGC	1 cut(s)	474
FspBI	CTAG	2 cut(s)	1038, 1193
GlaI	GCGC	1 cut(s)	417
GluI	GCNGC	1 cut(s)	474
HaeII	RGCGCY	1 cut(s)	419
HaeIII	GGCC	2 cut(s)	513, 904
HapII	CCGG	1 cut(s)	1147
HgaI	GACGC	3 cut(s)	164, 236, 399
HhaI	GCGC	1 cut(s)	418
Hin1I	GRCGYC	1 cut(s)	391
Hin1II	CATG	2 cut(s)	925, 1338
Hin6I	GCGC	1 cut(s)	416
HinP1I	GCGC	1 cut(s)	416
HincII	GTYRAC	3 cut(s)	226, 371, 690
HindII	GTYRAC	3 cut(s)	226, 371, 690
HindIII	AAGCTT	1 cut(s)	610
HpaII	CCGG	1 cut(s)	1147
HphI	GGTGA	3 cut(s)	641, 716, 1015
Hpy188III	TCNNGA	2 cut(s)	131, 1112
Hpy99I	CGWCG	1 cut(s)	1113
HpyAV	CCTTC	5 cut(s)	28, 128, 173, 404, 546
HpyCH4III	ACNGT	4 cut(s)	1135, 1168, 1342, 1352
HpyCH4IV	ACGT	4 cut(s)	47, 354, 880, 1108
HpyF10VI	GCNNNNNNNGC	2 cut(s)	470, 532
HpyF3I	CTNAG	4 cut(s)	170, 386, 589, 780
HpySE526I	ACGT	4 cut(s)	47, 354, 880, 1108
Hsp92I	GRCGYC	1 cut(s)	391
Hsp92II	CATG	2 cut(s)	925, 1338
HspAI	GCGC	1 cut(s)	416
Kzo9I	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
LguI	GCTCTTC	1 cut(s)	1388
LmnI	GCTCC	5 cut(s)	235, 413, 469, 801, 1072
Lsp1109I	GCAGC	1 cut(s)	460
LweI	GCATC	1 cut(s)	810
MaeI	CTAG	2 cut(s)	1038, 1193
MaeII	ACGT	4 cut(s)	47, 354, 880, 1108
MaeIII	GTNAC	3 cut(s)	358, 1104, 1342
MalI	GATC	7 cut(s)	267, 486, 568, 679, 733, 843, 1017
MboI	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
MboII	GAAGA	7 cut(s)	77, 115, 455, 458, 1018, 1133, 1375
MfeI	CAATTG	1 cut(s)	660
MflI	RGATCY	5 cut(s)	265, 484, 566, 677, 841
MhlI	GDGCHC	5 cut(s)	214, 466, 625, 779, 985
MlyI	GAGTC	5 cut(s)	581, 740, 860, 923, 1255
MmeI	TCCRAC	2 cut(s)	377, 833
MnlI	CCTC	6 cut(s)	52, 125, 331, 453, 586, 1224
Mph1103I	ATGCAT	1 cut(s)	1336
MseI	TTAA	2 cut(s)	989, 1137
MslI	CAYNNNNRTG	1 cut(s)	245
MspA1I	CMGCKG	1 cut(s)	1317
MspI	CCGG	1 cut(s)	1147
MspR9I	CCNGG	1 cut(s)	1326
MunI	CAATTG	1 cut(s)	660
Mva1269I	GAATGC	2 cut(s)	769, 896
MvaI	CCWGG	1 cut(s)	1326
MvnI	CGCG	1 cut(s)	526
MwoI	GCNNNNNNNGC	2 cut(s)	470, 532
NdeII	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
NlaIII	CATG	2 cut(s)	925, 1338
NlaIV	GGNNCC	3 cut(s)	465, 492, 984
NsiI	ATGCAT	1 cut(s)	1336
NspI	RCATGY	1 cut(s)	925
PciSI	GCTCTTC	1 cut(s)	1388
PctI	GAATGC	2 cut(s)	769, 896
PfeI	GAWTC	7 cut(s)	250, 316, 665, 752, 761, 1058, 1429
PkrI	GCNGC	1 cut(s)	475
PleI	GAGTC	5 cut(s)	581, 740, 860, 923, 1255
PpsI	GAGTC	5 cut(s)	581, 740, 860, 923, 1255
Ppu21I	YACGTR	2 cut(s)	48, 881
PshBI	ATTAAT	1 cut(s)	989
Psp6I	CCWGG	1 cut(s)	1324
PspEI	GGTNACC	1 cut(s)	358
PspGI	CCWGG	1 cut(s)	1324
PspN4I	GGNNCC	3 cut(s)	465, 492, 984
PspPI	GGNCC	2 cut(s)	903, 1211
PsuI	RGATCY	5 cut(s)	265, 484, 566, 677, 841
PvuII	CAGCTG	1 cut(s)	1317
RsaI	GTAC	2 cut(s)	453, 1248
RsaNI	GTAC	2 cut(s)	452, 1247
RseI	CAYNNNNRTG	1 cut(s)	245
SapI	GCTCTTC	1 cut(s)	1388
SaqAI	TTAA	2 cut(s)	989, 1137
SatI	GCNGC	1 cut(s)	474
Sau3AI	GATC	7 cut(s)	265, 484, 566, 677, 731, 841, 1015
Sau96I	GGNCC	2 cut(s)	903, 1211
SchI	GAGTC	5 cut(s)	581, 740, 860, 923, 1255
ScrFI	CCNGG	1 cut(s)	1326
SduI	GDGCHC	5 cut(s)	214, 466, 625, 779, 985
SfaNI	GCATC	1 cut(s)	810
SinI	GGWCC	1 cut(s)	1211
SmiMI	CAYNNNNRTG	1 cut(s)	245
SmlI	CTYRAG	2 cut(s)	726, 945
SmoI	CTYRAG	2 cut(s)	726, 945
SnaBI	TACGTA	2 cut(s)	48, 881
SsiI	CCGC	5 cut(s)	99, 104, 311, 524, 1180
SspI	AATATT	1 cut(s)	298
SspMI	CTAG	2 cut(s)	1038, 1193
StyD4I	CCNGG	1 cut(s)	1324
TaaI	ACNGT	4 cut(s)	1135, 1168, 1342, 1352
TaiI	ACGT	4 cut(s)	50, 357, 883, 1111
TaqI	TCGA	2 cut(s)	132, 505
TatI	WGTACW	1 cut(s)	1246
TfiI	GAWTC	7 cut(s)	250, 316, 665, 752, 761, 1058, 1429
Tru1I	TTAA	2 cut(s)	989, 1137
Tru9I	TTAA	2 cut(s)	989, 1137
TscAI	CASTG	3 cut(s)	354, 703, 1308
TseI	GCWGC	1 cut(s)	473
TspDTI	ATGAA	5 cut(s)	24, 27, 621, 753, 1086
TspRI	CASTG	3 cut(s)	354, 703, 1308
VneI	GTGCAC	1 cut(s)	621
VpaK11BI	GGWCC	1 cut(s)	1211
VspI	ATTAAT	1 cut(s)	989
XapI	RAATTY	5 cut(s)	556, 598, 645, 716, 1402
XceI	RCATGY	1 cut(s)	925
XcmI	CCANNNNNNNNNTGG	1 cut(s)	897
XspI	CTAG	2 cut(s)	1038, 1193
Zsp2I	ATGCAT	1 cut(s)	1336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.