Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007604

Physical Location & Seq

Genomic Coordinates Reverse (-)

10009716 .. 10016565

Gene Structure 6850 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1112900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGAAGATGCAGATGGACAATGGCAAGTACGTAAGGTACACGCCGGAGCAGGTGGAAGCTTTGGAGCGGTTGTACCATGAGTGCCCGAAGCCGAGCTCCATGCGCCGCCAGCAGCTCATCCGTGAGTGCCCTATCCTCTCCAACATCGAGCCTAAGCAGATCAAAGTCTGGTTCCAAAACCGCAGATGTAGAGAGAAGCAGCGGAAGGAGGCGTCGAGGTTGCAGACCGTGAACAGAAAGCTGACGGCGATGAATAAGCTTCTGATGGAGGAGAATGATCGGTTGCAGAAGCAGGTGTCTCAGCTGGTCTACGAGAACAGCTACTTCCGCCAACAGACACAAAATGCGACTTTAGCCACGACAGACACAAGTTGTGATTCGGTGGTGACGAGCGGTCAACACCATTTGACTCCGCAGCAGCATCCACCACCACCGCCAAGGGATGCGAGCCCTGCAGGACTTCTGTCCATTGCAGAGGAAACTTTAGCAGAGTTTTTATCAAAGGCCACTGGGACTGCTGTGGAGTGGGTCCAATTGCCTGGGATGAAGCCTGGTCCGGATTCCATTGGAATCGTTGCAATTTCTCACGGTTGCACCGGTGTGGCAGCACGTGCATGCGGCCTTGTAGGTCTAGACCCTACAAGAGTCGCTGAAATCCTCAAAGATCGGCCTTCGTGGTTCCGAAACTGCCGATCTGTGGATGTGCTGAACGTGTTGTCCACTGGCAATGGTGGAACCATTGAACTGCTTTACATGCAGCTCTATGCACCCACAACTTTGGCTCCAGCTCGAGATTTCTGGTTGCTTCGCTACACATCGGTACTAGAAGATGGTAGTCTCGTGGTCTGTGAAAGATCACTTAACAACACACAGAATGGTCCTAGCATGCCACCGGTGCAGAATTTTGTGAGAGCAGAAATGCTGCCGAGCGGGTATCTAATTAGACCTTGTGAAGGTGGTGGCTCGATCCTTCACATTGTTGATCATATGGATTTGGAGCCTTGGAGTGTACCCGAAGTGTTGCGCCCGCTTTATGAGTCATCAACACTTCTTGCTCAGAAGACAACTATGGCGGCATTACGCAACCTGAGGCAGATTTCTCAAGAAGTTTCTCAGCCTAATTCCGCTGGTTGGGGAAGAAGGCCTGCAGCTCTCCGTGCTCTTAGTCAGAGATTGAGCAAAGGTTTCAATGAAGCAGTTAACGGGTTTACAGATGAGGGATGGTCTGTTCTAGAAAGTGATGGTGTTGATGATGTTACCCTTCTTGTGAACTCGTCTCCGGGCAAGATGATGAGTGCAAATCTTTATACCAATGGAGTTCCATCTATGAGCACTGCAGTCTTATGTGCCAAAGCATCCATGTTACTCCAAAATGTGCCTCCAGCCATACTTCTTAGATTCTTGCGAGAACACCGATCAGAGTGGGCTGACAGAAGCATTGATGCTTATTCAGCTGCTGCTATCAAAGCTGGTCCCTGCAACATGCTAGGGCCTCGAGCTGGAAGTTTCGGGGATCAGGTTATTCTTCCCCTGGCTCACACAATTGAGCATGAAGAGTTCATGGAGGTCATTAAGATTGAAAACATGGGCCACTATCGAGAGGATATGATGATGCCTGCAGCTGACATTTTCCTCTTGCAACTGTGCAGCGGAGTGGATGAGAATGCCGTCGGGACCTGCGCTGAACTAGTATTTGCTCCCATTGATGCATCATTTTCGGATGATGCTCCAATTCTACCTTCTGGTTTTCGCATCATCCCTCTTGATTCTCGGATGGATACTCCGAGTCCAAACCGTACGCTTGATCTTGCCTCAGCTCTTGAAGTTGGACCTGCAGGAAGCAGAGCATCTGGTGATAATGCTGGCCACTCTGGCAATACAAAGTCTGTGATGACAATAGCATTCCAGTTTGCATTTGAAATTCACCTCCAAGAAAATATAGCCGCCATGGCTCGGCAGTATGTGCGCAGTATCATAGCATCAGTGCAGAGGGTGGCACTGGCACTCTCCCCGTCTCATTTTGGTTCGCATGCTGGTTTCCGGCCTCCACCTGGCACCCCTGAAGCACAAACACTTGCTGGTTGGATCTGTCAGAGCTATAGGTGCTATCTGGGTGGAGAACTACTAAAAACCGAAGGAAGCGAGTCCATTCTCAAATCCCTTTGGCATCACTCGGATGCAATCTTATGTTGCTCCTTGAAGGCAATGCCAGTTTTTACATTCGCAAACCAGGCAGGCCTTGACATGCTTGAGACGACGTTAGTTGCACTTCAAGACATAACACTGGAGAAGATTTTTGATGACAACGGAAGGAAGACCCTATGCTCCGAGTTCCCGCAGATAATGCAGCAGGGTTTTATGTGTCTTCAAGGAGGAATATGCATGTCAAGCATGGGAAGGCCGATCTCGTACGAGAGAGCAGTGGCATGGAAGGTGTTGAACGAAGAAGAAACTGCTCACTGCATCTGCTTCATGTTCATCAACTGGTCATTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0008284 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009933 GO:0010014 GO:0010016 GO:0010033 GO:0010065 GO:0010067 GO:0010072 GO:0010087 GO:0010089 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045595 GO:0045597 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048518 GO:0048522 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090421 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox MEKHLA START

Protein Analysis

833

Amino Acids

91.75

Weight (kDa)

5.9

Isoelectric Point (pI)

50.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	9 - 67	4.6e-16	Homeodomain
START	PF01852	157 - 365	3.5e-55	START domain
MEKHLA	PF08670	691 - 831	3.4e-48	MEKHLA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32880
fragaria_vesca	FvH4_2g35360 FvH4_2g35360 FvH4_2g35360 FvH4_2g35360
malus_domestica	MD08G1112900.v1.1 MD15G1092200.v1.1
prunus_persica	Prupe.1G447100_v2.0.a1 Prupe.1G447100_v2.0.a1
pyrus_communis	pycom08g09360 pycom110g00010 pycom15g08570
rosa_chinensis	RchiOBHm_Chr6g0306551
rosa_laevigata	RLG00000010786
rosa_multiflora	Rmu_sc0002971.1_g000003 Rmu_sc0002971.1_g000004
rosa_roxburghii	Rroxscaffold_7G00161680
rosa_rugosa	Rorug06G0350500 Rorug06G0350500
rosa_samantha	Rh6AG462700 Rh6BG438100 Rh6CG476800 Rh6DG463500
rosa_wichuraiana	Rw6G040320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	40, 283
Acc16I	TGCGCA	1 cut(s)	1967
Acc36I	ACCTGC	4 cut(s)	40, 283, 1685, 1840
AccB1I	GGYRCC	1 cut(s)	2054
AccBSI	CCGCTC	3 cut(s)	67, 393, 930
AccI	GTMKAC	1 cut(s)	309
AccIII	TCCGGA	1 cut(s)	556
AclWI	GGATC	3 cut(s)	961, 1521, 2093
AcoI	YGGCCR	1 cut(s)	1864
AcsI	RAATTY	2 cut(s)	901, 1920
AcuI	CTGAAG	1 cut(s)	2082
AcvI	CACGTG	1 cut(s)	611
AcyI	GRCGYC	1 cut(s)	212
AfaI	GTAC	7 cut(s)	29, 38, 74, 822, 1011, 1798, 2411
AfiI	CCNNNNNNNGG	6 cut(s)	697, 953, 1131, 1499, 1953, 2051
AflIII	ACRYGT	1 cut(s)	711
AgeI	ACCGGT	2 cut(s)	596, 892
AgsI	TTSAA	9 cut(s)	743, 1189, 1580, 1823, 1919, 2200, 2273, 2369, 2440
AhdI	GACNNNNNGTC	1 cut(s)	393
AhlI	ACTAGT	1 cut(s)	1687
AjnI	CCWGG	5 cut(s)	538, 550, 1530, 2050, 2229
AjuI	GAANNNNNNNTTGG	2 cut(s)	1723, 1755
AleI	CACNNNNGTG	1 cut(s)	599
AloI	GAACNNNNNNTCC	4 cut(s)	701, 733, 1212, 1244
Alw21I	GWGCWC	3 cut(s)	98, 1162, 1334
Alw26I	GTCTC	5 cut(s)	303, 842, 1281, 2019, 2246
AlwI	GGATC	3 cut(s)	961, 1521, 2093
AlwNI	CAGNNNCTG	1 cut(s)	1457
Ama87I	CYCGRG	2 cut(s)	789, 1494
Aor13HI	TCCGGA	1 cut(s)	556
ApoI	RAATTY	2 cut(s)	901, 1920
AsiGI	ACCGGT	2 cut(s)	596, 892
AspLEI	GCGC	4 cut(s)	105, 1026, 1682, 1968
AspS9I	GGNCC	8 cut(s)	529, 554, 878, 1472, 1490, 1588, 1674, 1829
AsuC2I	CCSGG	1 cut(s)	1281
AsuHPI	GGTGA	3 cut(s)	397, 1865, 1916
AvaI	CYCGRG	2 cut(s)	789, 1494
AvaII	GGWCC	6 cut(s)	529, 554, 878, 1472, 1674, 1829
AxyI	CCTNAGG	1 cut(s)	1088
BaeGI	GKGCMC	2 cut(s)	86, 131
BalI	TGGCCA	1 cut(s)	1866
BanI	GGYRCC	1 cut(s)	2054
BanII	GRGCYC	2 cut(s)	98, 452
BauI	CACGAG	1 cut(s)	839
BbrPI	CACGTG	1 cut(s)	611
BbsI	GAAGAC	3 cut(s)	1067, 2321, 2357
Bbv12I	GWGCWC	3 cut(s)	98, 1162, 1334
BbvCI	CCTCAGC	1 cut(s)	1813
BccI	CCATC	7 cut(s)	7, 259, 824, 1215, 1235, 1330, 1768
BceAI	ACGGC	2 cut(s)	261, 1652
BcgI	CGANNNNNNTGC	4 cut(s)	82, 116, 1698, 1732
BciT130I	CCWGG	5 cut(s)	540, 552, 1532, 2052, 2231
BciVI	GTATCC	1 cut(s)	1771
BclI	TGATCA	1 cut(s)	982
BcnI	CCSGG	1 cut(s)	1281
BcoDI	GTCTC	5 cut(s)	303, 842, 1281, 2019, 2246
BcuI	ACTAGT	1 cut(s)	1687
BfaI	CTAG	6 cut(s)	632, 824, 882, 1232, 1487, 1688
BfmI	CTRYAG	6 cut(s)	453, 1146, 1335, 1617, 1833, 2098
BfuAI	ACCTGC	4 cut(s)	40, 283, 1685, 1840
BfuI	GTATCC	1 cut(s)	1771
BglI	GCCNNNNNGGC	2 cut(s)	1872, 1949
Bme1390I	CCNGG	6 cut(s)	540, 552, 1281, 1532, 2052, 2231
Bme18I	GGWCC	6 cut(s)	529, 554, 878, 1472, 1674, 1829
BmeRI	GACNNNNNGTC	1 cut(s)	393
BmeT110I	CYCGRG	2 cut(s)	789, 1494
BmgT120I	GGNCC	8 cut(s)	529, 554, 878, 1472, 1490, 1588, 1674, 1829
BmiI	GGNNCC	9 cut(s)	173, 530, 680, 736, 783, 999, 1474, 1675, 2056
BmrFI	CCNGG	6 cut(s)	540, 552, 1281, 1532, 2052, 2231
BmrI	ACTGGG	1 cut(s)	519
BmuI	ACTGGG	1 cut(s)	519
BoxI	GACNNNNGTC	1 cut(s)	463
BpiI	GAAGAC	3 cut(s)	1067, 2321, 2357
BpmI	CTGGAG	3 cut(s)	768, 1365, 2306
Bpu10I	CCTNAGC	2 cut(s)	153, 1813
BpuEI	CTTGAG	2 cut(s)	1086, 2270
BpuMI	CCSGG	1 cut(s)	1281
BsaAI	YACGTR	2 cut(s)	31, 611
BsaHI	GRCGYC	1 cut(s)	212
BsaJI	CCNNGG	5 cut(s)	437, 539, 1001, 1530, 1947
BsaWI	WCCGGW	3 cut(s)	556, 596, 892
BsaXI	ACNNNNNCTCC	4 cut(s)	56, 86, 766, 796
Bsc4I	CCNNNNNNNGG	6 cut(s)	697, 953, 1131, 1499, 1953, 2051
Bse118I	RCCGGY	2 cut(s)	596, 892
Bse1I	ACTGG	7 cut(s)	514, 727, 1906, 2004, 2210, 2289, 2489
Bse21I	CCTNAGG	1 cut(s)	1088
Bse3DI	GCAATG	3 cut(s)	468, 733, 2211
BseAI	TCCGGA	1 cut(s)	556
BseBI	CCWGG	5 cut(s)	540, 552, 1532, 2052, 2231
BseDI	CCNNGG	5 cut(s)	437, 539, 1001, 1530, 1947
BseLI	CCNNNNNNNGG	6 cut(s)	697, 953, 1131, 1499, 1953, 2051
BseMI	GCAATG	3 cut(s)	468, 733, 2211
BseMII	CTCAG	5 cut(s)	314, 1070, 1079, 1127, 1827
BseNI	ACTGG	7 cut(s)	514, 727, 1906, 2004, 2210, 2289, 2489
BseRI	GAGGAG	1 cut(s)	284
BseSI	GKGCMC	2 cut(s)	86, 131
BsgI	GTGCAG	3 cut(s)	917, 1666, 2006
BshNI	GGYRCC	1 cut(s)	2054
BshTI	ACCGGT	2 cut(s)	596, 892
BsiHKAI	GWGCWC	3 cut(s)	98, 1162, 1334
BsiHKCI	CYCGRG	2 cut(s)	789, 1494
BsiSI	CCGG	6 cut(s)	44, 557, 597, 893, 1280, 2041
BsiWI	CGTACG	2 cut(s)	1796, 2409
BslFI	GGGAC	3 cut(s)	526, 1458, 1687
BslI	CCNNNNNNNGG	6 cut(s)	697, 953, 1131, 1499, 1953, 2051
BsmAI	GTCTC	5 cut(s)	303, 842, 1281, 2019, 2246
BsmBI	CGTCTC	3 cut(s)	1281, 2019, 2246
BsmFI	GGGAC	3 cut(s)	526, 1458, 1687
BsmI	GAATGC	2 cut(s)	1669, 1901
BsoBI	CYCGRG	2 cut(s)	789, 1494
Bsp1286I	GDGCHC	6 cut(s)	86, 98, 131, 452, 1162, 1334
Bsp13I	TCCGGA	1 cut(s)	556
Bsp19I	CCATGG	1 cut(s)	1947
BspCNI	CTCAG	5 cut(s)	313, 1069, 1080, 1126, 1826
BspEI	TCCGGA	1 cut(s)	556
BspLI	GGNNCC	9 cut(s)	173, 530, 680, 736, 783, 999, 1474, 1675, 2056
BspMAI	CTGCAG	5 cut(s)	457, 1150, 1339, 1621, 1837
BspMI	ACCTGC	4 cut(s)	40, 283, 1685, 1840
BspPI	GGATC	3 cut(s)	961, 1521, 2093
BspT107I	GGYRCC	1 cut(s)	2054
BsrBI	CCGCTC	3 cut(s)	67, 393, 930
BsrDI	GCAATG	3 cut(s)	468, 733, 2211
BsrFI	RCCGGY	2 cut(s)	596, 892
BsrI	ACTGG	7 cut(s)	514, 727, 1906, 2004, 2210, 2289, 2489
BssAI	RCCGGY	2 cut(s)	596, 892
BssECI	CCNNGG	5 cut(s)	437, 539, 1001, 1530, 1947
BssNI	GRCGYC	1 cut(s)	212
BssSI	CACGAG	1 cut(s)	839
BssT1I	CCWWGG	3 cut(s)	437, 1001, 1947
Bst2BI	CACGAG	1 cut(s)	839
Bst2UI	CCWGG	5 cut(s)	540, 552, 1532, 2052, 2231
Bst4CI	ACNGT	4 cut(s)	229, 590, 1644, 1796
Bst6I	CTCTTC	1 cut(s)	1548
BstACI	GRCGYC	1 cut(s)	212
BstAPI	GCANNNNNTGC	3 cut(s)	611, 1963, 2344
BstBAI	YACGTR	2 cut(s)	31, 611
BstDEI	CTNAG	8 cut(s)	153, 300, 1056, 1088, 1113, 1163, 1394, 1813
BstDSI	CCRYGG	1 cut(s)	1947
BstENI	CCTNNNNNAGG	1 cut(s)	951
BstHHI	GCGC	4 cut(s)	105, 1026, 1682, 1968
BstMAI	GTCTC	5 cut(s)	303, 842, 1281, 2019, 2246
BstNI	CCWGG	5 cut(s)	540, 552, 1532, 2052, 2231
BstNSI	RCATGY	7 cut(s)	618, 757, 889, 1486, 2033, 2248, 2386
BstPAI	GACNNNNGTC	1 cut(s)	463
BstSCI	CCNGG	6 cut(s)	538, 550, 1279, 1530, 2050, 2229
BstSFI	CTRYAG	6 cut(s)	453, 1146, 1335, 1617, 1833, 2098
BstSLI	GKGCMC	2 cut(s)	86, 131
BstSNI	TACGTA	1 cut(s)	31
BstV2I	GAAGAC	3 cut(s)	1067, 2321, 2357
BstX2I	RGATCY	1 cut(s)	2085
BstXI	CCANNNNNNTGG	2 cut(s)	539, 778
BstYI	RGATCY	1 cut(s)	2085
Bsu36I	CCTNAGG	1 cut(s)	1088
BsuI	GTATCC	1 cut(s)	1771
BtgI	CCRYGG	1 cut(s)	1947
BtgZI	GCGATG	1 cut(s)	263
BtsI	GCAGTG	3 cut(s)	1332, 2427, 2458
BtsIMutI	CAGTG	8 cut(s)	507, 720, 1332, 1989, 1997, 2282, 2427, 2458
BveI	ACCTGC	4 cut(s)	40, 283, 1685, 1840
CaiI	CAGNNNCTG	1 cut(s)	1457
CfoI	GCGC	4 cut(s)	105, 1026, 1682, 1968
Cfr10I	RCCGGY	2 cut(s)	596, 892
Cfr13I	GGNCC	8 cut(s)	529, 554, 878, 1472, 1490, 1588, 1674, 1829
CseI	GACGC	1 cut(s)	201
Csp6I	GTAC	7 cut(s)	28, 37, 73, 821, 1010, 1797, 2410
CspAI	ACCGGT	2 cut(s)	596, 892
CviQI	GTAC	7 cut(s)	28, 37, 73, 821, 1010, 1797, 2410
DdeI	CTNAG	8 cut(s)	153, 300, 1056, 1088, 1113, 1163, 1394, 1813
DriI	GACNNNNNGTC	1 cut(s)	393
EaeI	YGGCCR	1 cut(s)	1864
Eam1104I	CTCTTC	1 cut(s)	1548
Eam1105I	GACNNNNNGTC	1 cut(s)	393
EarI	CTCTTC	1 cut(s)	1548
EciI	GGCGGA	1 cut(s)	317
Ecl136II	GAGCTC	1 cut(s)	96
Eco105I	TACGTA	1 cut(s)	31
Eco130I	CCWWGG	3 cut(s)	437, 1001, 1947
Eco147I	AGGCCT	2 cut(s)	1144, 2238
Eco24I	GRGCYC	2 cut(s)	98, 452
Eco47I	GGWCC	6 cut(s)	529, 554, 878, 1472, 1674, 1829
Eco53kI	GAGCTC	1 cut(s)	96
Eco57I	CTGAAG	1 cut(s)	2082
Eco72I	CACGTG	1 cut(s)	611
Eco81I	CCTNAGG	1 cut(s)	1088
Eco88I	CYCGRG	2 cut(s)	789, 1494
EcoICRI	GAGCTC	1 cut(s)	96
EcoNI	CCTNNNNNAGG	1 cut(s)	951
EcoO109I	RGGNCCY	2 cut(s)	1490, 1674
EcoRII	CCWGG	5 cut(s)	538, 550, 1530, 2050, 2229
EcoT14I	CCWWGG	3 cut(s)	437, 1001, 1947
EcoT22I	ATGCAT	2 cut(s)	1711, 2384
EcoT38I	GRGCYC	2 cut(s)	98, 452
ErhI	CCWWGG	3 cut(s)	437, 1001, 1947
Esp3I	CGTCTC	3 cut(s)	1281, 2019, 2246
FaqI	GGGAC	3 cut(s)	526, 1458, 1687
FauI	CCCGC	3 cut(s)	923, 1035, 2343
FauNDI	CATATG	1 cut(s)	987
FbaI	TGATCA	1 cut(s)	982
FblI	GTMKAC	1 cut(s)	309
FriOI	GRGCYC	2 cut(s)	98, 452
FspBI	CTAG	6 cut(s)	632, 824, 882, 1232, 1487, 1688
FspI	TGCGCA	1 cut(s)	1967
GlaI	GCGC	4 cut(s)	104, 1025, 1681, 1967
GsuI	CTGGAG	3 cut(s)	768, 1365, 2306
HapII	CCGG	6 cut(s)	44, 557, 597, 893, 1280, 2041
HgaI	GACGC	1 cut(s)	201
HhaI	GCGC	4 cut(s)	105, 1026, 1682, 1968
Hin1I	GRCGYC	1 cut(s)	212
Hin6I	GCGC	4 cut(s)	103, 1024, 1680, 1966
HinP1I	GCGC	4 cut(s)	103, 1024, 1680, 1966
HincII	GTYRAC	2 cut(s)	398, 1201
HindII	GTYRAC	2 cut(s)	398, 1201
HindIII	AAGCTT	2 cut(s)	57, 257
HpaI	GTTAAC	1 cut(s)	1201
HpaII	CCGG	6 cut(s)	44, 557, 597, 893, 1280, 2041
HphI	GGTGA	3 cut(s)	397, 1865, 1916
Hpy166II	GTNNAC	9 cut(s)	39, 232, 310, 398, 720, 1010, 1201, 1209, 1270
Hpy8I	GTNNAC	9 cut(s)	39, 232, 310, 398, 720, 1010, 1201, 1209, 1270
Hpy99I	CGWCG	3 cut(s)	217, 1673, 2260
HpyCH4III	ACNGT	4 cut(s)	229, 590, 1644, 1796
HpyCH4IV	ACGT	4 cut(s)	30, 610, 711, 2258
HpyF3I	CTNAG	8 cut(s)	153, 300, 1056, 1088, 1113, 1163, 1394, 1813
HpySE526I	ACGT	4 cut(s)	30, 610, 711, 2258
Hsp92I	GRCGYC	1 cut(s)	212
HspAI	GCGC	4 cut(s)	103, 1024, 1680, 1966
Kpn2I	TCCGGA	1 cut(s)	556
Ksp22I	TGATCA	1 cut(s)	982
KspAI	GTTAAC	1 cut(s)	1201
LmnI	GCTCC	9 cut(s)	46, 64, 101, 787, 997, 1702, 1732, 2198, 2330
MaeI	CTAG	6 cut(s)	632, 824, 882, 1232, 1487, 1688
MaeII	ACGT	4 cut(s)	30, 610, 711, 2258
MaeIII	GTNAC	3 cut(s)	385, 1255, 1362
MbiI	CCGCTC	3 cut(s)	67, 393, 930
MfeI	CAATTG	2 cut(s)	533, 1542
MflI	RGATCY	1 cut(s)	2085
MhlI	GDGCHC	6 cut(s)	86, 98, 131, 452, 1162, 1334
MlsI	TGGCCA	1 cut(s)	1866
MluCI	AATT	8 cut(s)	533, 579, 901, 939, 1120, 1542, 1731, 1920
MluNI	TGGCCA	1 cut(s)	1866
MlyI	GAGTC	5 cut(s)	403, 654, 1046, 1795, 2153
MmeI	TCCRAC	3 cut(s)	165, 1807, 2063
Mox20I	TGGCCA	1 cut(s)	1866
Mph1103I	ATGCAT	2 cut(s)	1711, 2384
MroI	TCCGGA	1 cut(s)	556
MscI	TGGCCA	1 cut(s)	1866
MseI	TTAA	4 cut(s)	861, 1200, 1572, 2497
MslI	CAYNNNNRTG	3 cut(s)	599, 613, 2175
Msp20I	TGGCCA	1 cut(s)	1866
MspA1I	CMGCKG	6 cut(s)	202, 304, 1127, 1454, 1622, 1650
MspI	CCGG	6 cut(s)	44, 557, 597, 893, 1280, 2041
MspR9I	CCNGG	6 cut(s)	540, 552, 1281, 1532, 2052, 2231
MunI	CAATTG	2 cut(s)	533, 1542
Mva1269I	GAATGC	2 cut(s)	1669, 1901
MvaI	CCWGG	5 cut(s)	540, 552, 1532, 2052, 2231
NciI	CCSGG	1 cut(s)	1281
NcoI	CCATGG	1 cut(s)	1947
NdeI	CATATG	1 cut(s)	987
NlaIV	GGNNCC	9 cut(s)	173, 530, 680, 736, 783, 999, 1474, 1675, 2056
NmeAIII	GCCGAG	3 cut(s)	117, 951, 1933
NmuCI	GTSAC	1 cut(s)	385
NsbI	TGCGCA	1 cut(s)	1967
NsiI	ATGCAT	2 cut(s)	1711, 2384
NspI	RCATGY	7 cut(s)	618, 757, 889, 1486, 2033, 2248, 2386
OliI	CACNNNNGTG	1 cut(s)	599
PaeI	GCATGC	3 cut(s)	618, 889, 2033
PaeR7I	CTCGAG	2 cut(s)	789, 1494
PaqCI	CACCTGC	2 cut(s)	40, 283
PceI	AGGCCT	2 cut(s)	1144, 2238
PcsI	WCGNNNNNNNCGW	1 cut(s)	386
PctI	GAATGC	2 cut(s)	1669, 1901
PfeI	GAWTC	5 cut(s)	377, 560, 570, 1398, 1766
Pfl23II	CGTACG	2 cut(s)	1796, 2409
PinAI	ACCGGT	2 cut(s)	596, 892
PleI	GAGTC	5 cut(s)	403, 653, 1045, 1794, 2152
PmaCI	CACGTG	1 cut(s)	611
PmlI	CACGTG	1 cut(s)	611
PpsI	GAGTC	5 cut(s)	403, 653, 1045, 1794, 2152
Ppu21I	YACGTR	2 cut(s)	31, 611
PpuMI	RGGWCCY	1 cut(s)	1674
PshAI	GACNNNNGTC	1 cut(s)	463
Psp124BI	GAGCTC	1 cut(s)	98
Psp5II	RGGWCCY	1 cut(s)	1674
Psp6I	CCWGG	5 cut(s)	538, 550, 1530, 2050, 2229
PspCI	CACGTG	1 cut(s)	611
PspGI	CCWGG	5 cut(s)	538, 550, 1530, 2050, 2229
PspLI	CGTACG	2 cut(s)	1796, 2409
PspN4I	GGNNCC	9 cut(s)	173, 530, 680, 736, 783, 999, 1474, 1675, 2056
PspPI	GGNCC	8 cut(s)	529, 554, 878, 1472, 1490, 1588, 1674, 1829
PspPPI	RGGWCCY	1 cut(s)	1674
PspXI	VCTCGAGB	1 cut(s)	1494
PstI	CTGCAG	5 cut(s)	457, 1150, 1339, 1621, 1837
PstNI	CAGNNNCTG	1 cut(s)	1457
PsuI	RGATCY	1 cut(s)	2085
PvuII	CAGCTG	3 cut(s)	304, 1454, 1622
RsaI	GTAC	7 cut(s)	29, 38, 74, 822, 1011, 1798, 2411
RsaNI	GTAC	7 cut(s)	28, 37, 73, 821, 1010, 1797, 2410
RseI	CAYNNNNRTG	3 cut(s)	599, 613, 2175
SacI	GAGCTC	1 cut(s)	98
SaqAI	TTAA	4 cut(s)	861, 1200, 1572, 2497
Sau96I	GGNCC	8 cut(s)	529, 554, 878, 1472, 1490, 1588, 1674, 1829
SbfI	CCTGCAGG	2 cut(s)	457, 1837
SchI	GAGTC	5 cut(s)	403, 654, 1046, 1795, 2153
ScrFI	CCNGG	6 cut(s)	540, 552, 1281, 1532, 2052, 2231
SdaI	CCTGCAGG	2 cut(s)	457, 1837
SduI	GDGCHC	6 cut(s)	86, 98, 131, 452, 1162, 1334
SfcI	CTRYAG	6 cut(s)	453, 1146, 1335, 1617, 1833, 2098
Sfr274I	CTCGAG	2 cut(s)	789, 1494
SgrAI	CRCCGGYG	2 cut(s)	596, 892
SinI	GGWCC	6 cut(s)	529, 554, 878, 1472, 1674, 1829
SlaI	CTCGAG	2 cut(s)	789, 1494
SmiMI	CAYNNNNRTG	3 cut(s)	599, 613, 2175
SmlI	CTYRAG	4 cut(s)	789, 1101, 1494, 2249
SmoI	CTYRAG	4 cut(s)	789, 1101, 1494, 2249
SnaBI	TACGTA	1 cut(s)	31
SpeI	ACTAGT	1 cut(s)	1687
SphI	GCATGC	3 cut(s)	618, 889, 2033
Sse8387I	CCTGCAGG	2 cut(s)	457, 1837
Sse9I	AATT	8 cut(s)	533, 579, 901, 939, 1120, 1542, 1731, 1920
SseBI	AGGCCT	2 cut(s)	1144, 2238
SspMI	CTAG	6 cut(s)	632, 824, 882, 1232, 1487, 1688
SstI	GAGCTC	1 cut(s)	98
StuI	AGGCCT	2 cut(s)	1144, 2238
StyD4I	CCNGG	6 cut(s)	538, 550, 1279, 1530, 2050, 2229
StyI	CCWWGG	3 cut(s)	437, 1001, 1947
TaaI	ACNGT	4 cut(s)	229, 590, 1644, 1796
TaiI	ACGT	4 cut(s)	33, 613, 714, 2261
TaqI	TCGA	6 cut(s)	147, 215, 790, 965, 1495, 1597
TasI	AATT	8 cut(s)	533, 579, 901, 939, 1120, 1542, 1731, 1920
TauI	GCSGC	4 cut(s)	108, 621, 1076, 1946
TfiI	GAWTC	5 cut(s)	377, 560, 570, 1398, 1766
Tru1I	TTAA	4 cut(s)	861, 1200, 1572, 2497
Tru9I	TTAA	4 cut(s)	861, 1200, 1572, 2497
TscAI	CASTG	8 cut(s)	514, 727, 1339, 1989, 2004, 2289, 2427, 2465
TseFI	GTSAC	1 cut(s)	385
Tsp45I	GTSAC	1 cut(s)	385
TspDTI	ATGAA	8 cut(s)	17, 266, 560, 1206, 1549, 1566, 2461, 2467
TspGWI	ACGGA	3 cut(s)	110, 1145, 2322
TspRI	CASTG	8 cut(s)	514, 727, 1339, 1989, 2004, 2289, 2427, 2465
VpaK11BI	GGWCC	6 cut(s)	529, 554, 878, 1472, 1674, 1829
XagI	CCTNNNNNAGG	1 cut(s)	951
XapI	RAATTY	2 cut(s)	901, 1920
XbaI	TCTAGA	2 cut(s)	631, 1231
XceI	RCATGY	7 cut(s)	618, 757, 889, 1486, 2033, 2248, 2386
XhoI	CTCGAG	2 cut(s)	789, 1494
XmiI	GTMKAC	1 cut(s)	309
XspI	CTAG	6 cut(s)	632, 824, 882, 1232, 1487, 1688
Zsp2I	ATGCAT	2 cut(s)	1711, 2384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.