Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007604

Physical Location & Seq

Genomic Coordinates Reverse (-)

6405400 .. 6412182

Gene Structure 6783 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1092200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGAAGATGCAGATGGACAATGGCAAGTACGTAAGGTACACGCCGGAGCAAGTGGAAGCTTTAGAGCGGTTGTACCATGAGTGCCCGAAGCCGAGCTCCATGCGCCGCCAGCAGCTCATCCGCGAGTGCCCTATCCTCTCCAACATCGAGCCGAAGCAGATCAAAGTTTGGTTCCAAAACCGCAGATGCAGAGAGAAGCAGCGCAAGGAGGCGTCGAGATTGCAGACGGTGAACAGAAAGCTGACGGCGATGAATAAGCTTCTGATGGAGGAGAATGATCGGTTGCAGAAGCAGGTTTCTCAGCTGGTCTATGAGAACACCTATTTCCGCCAGCACACACAAAATGCAACTCTAGCGACGACAGACACGAGTTGTGAGTCGGTGGTGACGAGCGGTCAACACCATTTGACTGCACAGCAGCATCCACCACCAAGGGATGCCAGCCCTGCAGGACTTCTGTCCATTGCAGAGGAAACTTTAGCAGAGTTTTTATCGAAGGCCACTGGGACTGCTGTGGAGTGGGTCCAATTGCCTGGGATGAAGCCTGGTCCGGATTCCATTGGAATCGTTGCAATTTCTCACGGTTGCACTGGTGTGGCAGCACGTGCATGCGGCCTTGTAGGTCTAGACCCTACAAGAGTCGCTGAAATCCTCAAAGATCGGCCTTCGTGGTTCCGAAATTGCCGATCTGTGGATGTGGTCAATGTAATGTCCACTGGCAATGGTGGAACCATTGAACTGCTTTACATGCAGCTCTATGCACCCACGACTTTGGCACCAGCTCGAGACTTCTGGTTGCTTCGCTACACATCGGTTCTAGAAGACGGTAGTCTTGTGGTCTGTGAAAGATCACTTAACAACACACAGAATGGTCCTAGCATGCCACCAGTGCAGAATTTTGTGAGAGCGGAAATGCTGCCGAGCGGGTATCTAATTAGACCTTGTGAAGGTGGTGGCTCGATCCTTCACATTGTTGATCATATGGATTTAGAGCCTTGGAGTGTACCTGAAGTGTTGCGCCCGCTTTATGAGTCATCAACAATTCTTGCTCAGAAGACAACTATGGCAGCATTACGCAACCTCAGGCAGATATCTCAAGAAGTTTCTCAGCCTAATTCTGCTGGTTGGGGAAGAAGGCCTGCAGCTCTCCGTGCTCTTAGTCAGAGATTGAGCAAGGGTTTCAATGAAGCAGTTAACGGGTTTACAGATGAGGGATGGTCTGTTCTAGAAAGTGATGGTGTCGATGATGTTACCCTTCTTGTGAACTCGTCTCCGAGCAAGATGATGAGTGCAAATCTTTATACCGATGGAGTTCCATCTATGAGCACTGCAGTCTTATGTGCCAAAGCATCCATGTTACTCCAAAATGTGCCTCCAGCCATCCTTCTCAGATTCTTGCGAGAACACCGATCAGAGTGGGCTGACAGAAGCATTGATGCTTATTCAGCTGCTGCTGTTAAAGCTGGTCCCTGCAACATGCTAGGGCCTCGAGTTGGAAGTTTCGGGGATCAGGTTATTCTTCCCCTGGCTCACACAATTGAGCACGAAGAGTTCATGGAGGTCATTAAGATTGAGAACATGGGCCACTATCGAGAGGATATGATTATGCCTGCAGCTGACATTTTCCTCTTGCAACTGTGTAGTGGAGTGGATGAGAATGCCGTCGGGACCTGCTCTGAACTAGTATTTGCTCCCATCGATGCATCATTTTCGGATGATGCTCCAATTCTACCTTCTGGTTTCCGGATCATCCCTCTTGATTCTCCGATGGATACTCCCAGTCCAAACCGTACGCTTGATCTGGCCTCAGCTCTTGAAGTTGGACCCGCAGGAAGCAGAGCATCTGGTGATAATGCTGGCCACTCTGGCAATACAAAGTCCGTAATGACAATAGCATTCCAGTTTGCATTTGAAATTCACCTACAAGAAAATGTAGCCTCCATGGCTCGGCAGTATGTGCGCAGTATCATAGCATCAGTGCAGAGGGTGGCACTGGCGCTCTCCCCTTCTCGTTTTGGTTCTCATGCTGGTTTCCGGCCTCCACCTGGCACTCCCCAAGCACAAACACTTGCTGGTTGGATTTGTCAGAGCTATAGGTGCTATCTAGGTGGAGAACTACTAAAAACCGAAGGAAGCGAGTCCATTCTCAAATCCCTTTGGCATCACCCGGATGCAATCTTATGCTACTCTTTGAAGGCAATGCCAATTTTTACATTCGGAAACCAGGCAGGCCTGGACATGCTTGAGACGACGTTAGTTGCACTTCAAGACATTACGCTGGAAAAGATTTTCGATGACAATGGAAGGAAGACACTATGCTCCGAGTTCCCGCAGATAATGCAGCAGGGTTTTATGTGTCATCAAGGAGGAATATGCATGTCAAGCATGGGAAGGCCGATCTCGTACGAGAGAGCGGTAGCATGGAAGGTGCTGAATGAAGAAGAAACCGCTCACTGCATCTGCTTCATGTTCATCAACTGGTCATTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0008284 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009933 GO:0010014 GO:0010016 GO:0010033 GO:0010065 GO:0010067 GO:0010072 GO:0010087 GO:0010089 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045595 GO:0045597 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048518 GO:0048522 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090421 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox MEKHLA START

Protein Analysis

831

Amino Acids

91.67

Weight (kDa)

5.93

Isoelectric Point (pI)

50.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	9 - 67	4.6e-16	Homeodomain
START	PF01852	155 - 363	3.7e-55	START domain
MEKHLA	PF08670	689 - 829	1.2e-47	MEKHLA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32880
fragaria_vesca	FvH4_2g35360 FvH4_2g35360 FvH4_2g35360 FvH4_2g35360
malus_domestica	MD08G1112900.v1.1 MD15G1092200.v1.1
prunus_persica	Prupe.1G447100_v2.0.a1 Prupe.1G447100_v2.0.a1
pyrus_communis	pycom08g09360 pycom110g00010 pycom15g08570
rosa_chinensis	RchiOBHm_Chr6g0306551
rosa_laevigata	RLG00000010786
rosa_multiflora	Rmu_sc0002971.1_g000003 Rmu_sc0002971.1_g000004
rosa_roxburghii	Rroxscaffold_7G00161680
rosa_rugosa	Rorug06G0350500 Rorug06G0350500
rosa_samantha	Rh6AG462700 Rh6BG438100 Rh6CG476800 Rh6DG463500
rosa_wichuraiana	Rw6G040320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1961
Acc36I	ACCTGC	2 cut(s)	283, 1679
AccB1I	GGYRCC	1 cut(s)	775
AccBSI	CCGCTC	6 cut(s)	67, 393, 908, 924, 2414, 2450
AccII	CGCG	1 cut(s)	123
AccIII	TCCGGA	2 cut(s)	550, 1743
AclWI	GGATC	3 cut(s)	955, 1515, 1754
AcoI	YGGCCR	1 cut(s)	1858
AcsI	RAATTY	2 cut(s)	895, 1914
AcuI	CTGAAG	1 cut(s)	1029
AcvI	CACGTG	1 cut(s)	605
AcyI	GRCGYC	1 cut(s)	212
AfaI	GTAC	6 cut(s)	29, 38, 74, 1005, 1792, 2405
AfiI	CCNNNNNNNGG	5 cut(s)	691, 947, 1493, 1947, 2045
AgsI	TTSAA	6 cut(s)	737, 1183, 1817, 1913, 2194, 2267
AhdI	GACNNNNNGTC	1 cut(s)	393
AhlI	ACTAGT	1 cut(s)	1681
AjnI	CCWGG	6 cut(s)	532, 544, 1524, 2044, 2223, 2232
AjuI	GAANNNNNNNTTGG	2 cut(s)	1717, 1749
AleI	CACNNNNGTG	1 cut(s)	593
AloI	GAACNNNNNNTCC	2 cut(s)	1206, 1238
Alw21I	GWGCWC	4 cut(s)	98, 1156, 1328, 1545
Alw26I	GTCTC	3 cut(s)	780, 1275, 2240
AlwI	GGATC	3 cut(s)	955, 1515, 1754
AlwNI	CAGNNNCTG	1 cut(s)	1451
Ama87I	CYCGRG	2 cut(s)	783, 1488
Aor13HI	TCCGGA	2 cut(s)	550, 1743
ApoI	RAATTY	2 cut(s)	895, 1914
AspLEI	GCGC	5 cut(s)	105, 204, 1020, 1962, 1999
AspS9I	GGNCC	8 cut(s)	523, 548, 872, 1466, 1484, 1582, 1668, 1823
AsuC2I	CCSGG	1 cut(s)	2168
AsuHPI	GGTGA	5 cut(s)	241, 397, 1859, 1910, 2156
AvaI	CYCGRG	2 cut(s)	783, 1488
AvaII	GGWCC	6 cut(s)	523, 548, 872, 1466, 1668, 1823
AxyI	CCTNAGG	1 cut(s)	1082
BaeGI	GKGCMC	2 cut(s)	86, 131
BalI	TGGCCA	1 cut(s)	1860
BanI	GGYRCC	1 cut(s)	775
BanII	GRGCYC	1 cut(s)	98
BauI	CACGAG	1 cut(s)	367
BbrPI	CACGTG	1 cut(s)	605
BbsI	GAAGAC	3 cut(s)	828, 1061, 2315
Bbv12I	GWGCWC	4 cut(s)	98, 1156, 1328, 1545
BbvCI	CCTCAGC	1 cut(s)	1807
BccI	CCATC	9 cut(s)	7, 259, 1209, 1229, 1301, 1324, 1388, 1703, 1762
BceAI	ACGGC	2 cut(s)	261, 1646
BcgI	CGANNNNNNTGC	4 cut(s)	82, 116, 1692, 1726
BciT130I	CCWGG	6 cut(s)	534, 546, 1526, 2046, 2225, 2234
BciVI	GTATCC	1 cut(s)	1765
BclI	TGATCA	1 cut(s)	976
BcnI	CCSGG	1 cut(s)	2168
BcoDI	GTCTC	3 cut(s)	780, 1275, 2240
BcuI	ACTAGT	1 cut(s)	1681
BfaI	CTAG	8 cut(s)	353, 626, 818, 876, 1226, 1481, 1682, 2105
BfmI	CTRYAG	5 cut(s)	447, 1140, 1329, 1611, 2092
BfoI	RGCGCY	1 cut(s)	2000
BfuAI	ACCTGC	2 cut(s)	283, 1679
BfuI	GTATCC	1 cut(s)	1765
BglI	GCCNNNNNGGC	2 cut(s)	1866, 1943
Bme1390I	CCNGG	7 cut(s)	534, 546, 1526, 2046, 2168, 2225, 2234
Bme18I	GGWCC	6 cut(s)	523, 548, 872, 1466, 1668, 1823
BmeRI	GACNNNNNGTC	1 cut(s)	393
BmeT110I	CYCGRG	2 cut(s)	783, 1488
BmgT120I	GGNCC	8 cut(s)	523, 548, 872, 1466, 1484, 1582, 1668, 1823
BmiI	GGNNCC	8 cut(s)	173, 524, 674, 730, 777, 1468, 1669, 1825
BmrFI	CCNGG	7 cut(s)	534, 546, 1526, 2046, 2168, 2225, 2234
BmrI	ACTGGG	2 cut(s)	513, 1773
BmuI	ACTGGG	2 cut(s)	513, 1773
BoxI	GACNNNNGTC	2 cut(s)	457, 828
BpiI	GAAGAC	3 cut(s)	828, 1061, 2315
BpmI	CTGGAG	1 cut(s)	1359
Bpu10I	CCTNAGC	1 cut(s)	1807
BpuEI	CTTGAG	2 cut(s)	1080, 2264
BpuMI	CCSGG	1 cut(s)	2168
Bsa29I	ATCGAT	1 cut(s)	1698
BsaAI	YACGTR	2 cut(s)	31, 605
BsaHI	GRCGYC	1 cut(s)	212
BsaJI	CCNNGG	5 cut(s)	431, 533, 995, 1524, 1941
BsaWI	WCCGGW	2 cut(s)	550, 1743
Bsc4I	CCNNNNNNNGG	5 cut(s)	691, 947, 1493, 1947, 2045
Bse1I	ACTGG	8 cut(s)	508, 595, 721, 887, 1779, 1900, 1998, 2483
Bse21I	CCTNAGG	1 cut(s)	1082
Bse3DI	GCAATG	3 cut(s)	462, 727, 2205
BseAI	TCCGGA	2 cut(s)	550, 1743
BseBI	CCWGG	6 cut(s)	534, 546, 1526, 2046, 2225, 2234
BseCI	ATCGAT	1 cut(s)	1698
BseDI	CCNNGG	5 cut(s)	431, 533, 995, 1524, 1941
BseLI	CCNNNNNNNGG	5 cut(s)	691, 947, 1493, 1947, 2045
BseMI	GCAATG	3 cut(s)	462, 727, 2205
BseMII	CTCAG	6 cut(s)	314, 1064, 1096, 1121, 1402, 1821
BseNI	ACTGG	8 cut(s)	508, 595, 721, 887, 1779, 1900, 1998, 2483
BseRI	GAGGAG	1 cut(s)	284
BseSI	GKGCMC	2 cut(s)	86, 131
BsgI	GTGCAG	3 cut(s)	396, 911, 2000
Bsh1236I	CGCG	1 cut(s)	123
BshNI	GGYRCC	1 cut(s)	775
BshVI	ATCGAT	1 cut(s)	1698
BsiHKAI	GWGCWC	4 cut(s)	98, 1156, 1328, 1545
BsiHKCI	CYCGRG	2 cut(s)	783, 1488
BsiSI	CCGG	5 cut(s)	44, 551, 1744, 2035, 2168
BsiWI	CGTACG	2 cut(s)	1790, 2403
BslFI	GGGAC	3 cut(s)	520, 1452, 1681
BslI	CCNNNNNNNGG	5 cut(s)	691, 947, 1493, 1947, 2045
BsmAI	GTCTC	3 cut(s)	780, 1275, 2240
BsmBI	CGTCTC	2 cut(s)	1275, 2240
BsmFI	GGGAC	3 cut(s)	520, 1452, 1681
BsmI	GAATGC	2 cut(s)	1663, 1895
BsoBI	CYCGRG	2 cut(s)	783, 1488
Bsp1286I	GDGCHC	6 cut(s)	86, 98, 131, 1156, 1328, 1545
Bsp13I	TCCGGA	2 cut(s)	550, 1743
Bsp19I	CCATGG	1 cut(s)	1941
BspCNI	CTCAG	6 cut(s)	313, 1063, 1095, 1120, 1401, 1820
BspDI	ATCGAT	1 cut(s)	1698
BspEI	TCCGGA	2 cut(s)	550, 1743
BspFNI	CGCG	1 cut(s)	123
BspLI	GGNNCC	8 cut(s)	173, 524, 674, 730, 777, 1468, 1669, 1825
BspMAI	CTGCAG	4 cut(s)	451, 1144, 1333, 1615
BspMI	ACCTGC	2 cut(s)	283, 1679
BspPI	GGATC	3 cut(s)	955, 1515, 1754
BspT107I	GGYRCC	1 cut(s)	775
BsrBI	CCGCTC	6 cut(s)	67, 393, 908, 924, 2414, 2450
BsrDI	GCAATG	3 cut(s)	462, 727, 2205
BsrI	ACTGG	8 cut(s)	508, 595, 721, 887, 1779, 1900, 1998, 2483
BssECI	CCNNGG	5 cut(s)	431, 533, 995, 1524, 1941
BssNI	GRCGYC	1 cut(s)	212
BssSI	CACGAG	1 cut(s)	367
BssT1I	CCWWGG	3 cut(s)	431, 995, 1941
Bst2BI	CACGAG	1 cut(s)	367
Bst2UI	CCWGG	6 cut(s)	534, 546, 1526, 2046, 2225, 2234
Bst4CI	ACNGT	5 cut(s)	229, 584, 827, 1638, 1790
Bst6I	CTCTTC	1 cut(s)	1542
BstACI	GRCGYC	1 cut(s)	212
BstAPI	GCANNNNNTGC	3 cut(s)	605, 1957, 2338
BstBAI	YACGTR	2 cut(s)	31, 605
BstDEI	CTNAG	7 cut(s)	300, 1050, 1082, 1107, 1157, 1388, 1807
BstDSI	CCRYGG	1 cut(s)	1941
BstENI	CCTNNNNNAGG	1 cut(s)	945
BstFNI	CGCG	1 cut(s)	123
BstH2I	RGCGCY	1 cut(s)	2000
BstHHI	GCGC	5 cut(s)	105, 204, 1020, 1962, 1999
BstMAI	GTCTC	3 cut(s)	780, 1275, 2240
BstNI	CCWGG	6 cut(s)	534, 546, 1526, 2046, 2225, 2234
BstNSI	RCATGY	6 cut(s)	612, 751, 883, 1480, 2242, 2380
BstPAI	GACNNNNGTC	2 cut(s)	457, 828
BstSCI	CCNGG	7 cut(s)	532, 544, 1524, 2044, 2166, 2223, 2232
BstSFI	CTRYAG	5 cut(s)	447, 1140, 1329, 1611, 2092
BstSLI	GKGCMC	2 cut(s)	86, 131
BstSNI	TACGTA	1 cut(s)	31
BstUI	CGCG	1 cut(s)	123
BstV2I	GAAGAC	3 cut(s)	828, 1061, 2315
BstXI	CCANNNNNNTGG	2 cut(s)	533, 772
Bsu15I	ATCGAT	1 cut(s)	1698
Bsu36I	CCTNAGG	1 cut(s)	1082
BsuI	GTATCC	1 cut(s)	1765
BsuTUI	ATCGAT	1 cut(s)	1698
BtgI	CCRYGG	1 cut(s)	1941
BtgZI	GCGATG	1 cut(s)	263
BtsI	GCAGTG	2 cut(s)	1326, 2452
BtsIMutI	CAGTG	8 cut(s)	501, 588, 714, 894, 1326, 1983, 1991, 2452
BveI	ACCTGC	2 cut(s)	283, 1679
CaiI	CAGNNNCTG	1 cut(s)	1451
CfoI	GCGC	5 cut(s)	105, 204, 1020, 1962, 1999
Cfr13I	GGNCC	8 cut(s)	523, 548, 872, 1466, 1484, 1582, 1668, 1823
ClaI	ATCGAT	1 cut(s)	1698
CseI	GACGC	1 cut(s)	201
Csp6I	GTAC	6 cut(s)	28, 37, 73, 1004, 1791, 2404
CviQI	GTAC	6 cut(s)	28, 37, 73, 1004, 1791, 2404
DdeI	CTNAG	7 cut(s)	300, 1050, 1082, 1107, 1157, 1388, 1807
DriI	GACNNNNNGTC	1 cut(s)	393
EaeI	YGGCCR	1 cut(s)	1858
Eam1104I	CTCTTC	1 cut(s)	1542
Eam1105I	GACNNNNNGTC	1 cut(s)	393
EarI	CTCTTC	1 cut(s)	1542
EciI	GGCGGA	1 cut(s)	317
Ecl136II	GAGCTC	1 cut(s)	96
Eco105I	TACGTA	1 cut(s)	31
Eco130I	CCWWGG	3 cut(s)	431, 995, 1941
Eco147I	AGGCCT	2 cut(s)	1138, 2232
Eco24I	GRGCYC	1 cut(s)	98
Eco32I	GATATC	1 cut(s)	1092
Eco47I	GGWCC	6 cut(s)	523, 548, 872, 1466, 1668, 1823
Eco53kI	GAGCTC	1 cut(s)	96
Eco57I	CTGAAG	1 cut(s)	1029
Eco72I	CACGTG	1 cut(s)	605
Eco81I	CCTNAGG	1 cut(s)	1082
Eco88I	CYCGRG	2 cut(s)	783, 1488
EcoICRI	GAGCTC	1 cut(s)	96
EcoNI	CCTNNNNNAGG	1 cut(s)	945
EcoO109I	RGGNCCY	2 cut(s)	1484, 1668
EcoRII	CCWGG	6 cut(s)	532, 544, 1524, 2044, 2223, 2232
EcoRV	GATATC	1 cut(s)	1092
EcoT14I	CCWWGG	3 cut(s)	431, 995, 1941
EcoT22I	ATGCAT	2 cut(s)	1705, 2378
EcoT38I	GRGCYC	1 cut(s)	98
ErhI	CCWWGG	3 cut(s)	431, 995, 1941
Esp3I	CGTCTC	2 cut(s)	1275, 2240
FaqI	GGGAC	3 cut(s)	520, 1452, 1681
FauI	CCCGC	4 cut(s)	917, 1029, 1834, 2337
FauNDI	CATATG	1 cut(s)	981
FbaI	TGATCA	1 cut(s)	976
FriOI	GRGCYC	1 cut(s)	98
FspBI	CTAG	8 cut(s)	353, 626, 818, 876, 1226, 1481, 1682, 2105
FspI	TGCGCA	1 cut(s)	1961
GlaI	GCGC	5 cut(s)	104, 203, 1019, 1961, 1998
GsuI	CTGGAG	1 cut(s)	1359
HaeII	RGCGCY	1 cut(s)	2000
HapII	CCGG	5 cut(s)	44, 551, 1744, 2035, 2168
HgaI	GACGC	1 cut(s)	201
HhaI	GCGC	5 cut(s)	105, 204, 1020, 1962, 1999
Hin1I	GRCGYC	1 cut(s)	212
Hin6I	GCGC	5 cut(s)	103, 202, 1018, 1960, 1997
HinP1I	GCGC	5 cut(s)	103, 202, 1018, 1960, 1997
HincII	GTYRAC	2 cut(s)	398, 1195
HindII	GTYRAC	2 cut(s)	398, 1195
HindIII	AAGCTT	2 cut(s)	57, 257
HinfI	GANTC	8 cut(s)	377, 554, 564, 639, 1031, 1392, 1760, 2138
HpaI	GTTAAC	1 cut(s)	1195
HpaII	CCGG	5 cut(s)	44, 551, 1744, 2035, 2168
HphI	GGTGA	5 cut(s)	241, 397, 1859, 1910, 2156
Hpy166II	GTNNAC	8 cut(s)	39, 232, 398, 714, 1004, 1195, 1203, 1264
Hpy8I	GTNNAC	8 cut(s)	39, 232, 398, 714, 1004, 1195, 1203, 1264
Hpy99I	CGWCG	4 cut(s)	217, 361, 1667, 2254
HpyCH4III	ACNGT	5 cut(s)	229, 584, 827, 1638, 1790
HpyCH4IV	ACGT	3 cut(s)	30, 604, 2252
HpyF3I	CTNAG	7 cut(s)	300, 1050, 1082, 1107, 1157, 1388, 1807
HpySE526I	ACGT	3 cut(s)	30, 604, 2252
Hsp92I	GRCGYC	1 cut(s)	212
HspAI	GCGC	5 cut(s)	103, 202, 1018, 1960, 1997
Kpn2I	TCCGGA	2 cut(s)	550, 1743
Ksp22I	TGATCA	1 cut(s)	976
KspAI	GTTAAC	1 cut(s)	1195
LmnI	GCTCC	5 cut(s)	46, 101, 1696, 1726, 2324
MaeI	CTAG	8 cut(s)	353, 626, 818, 876, 1226, 1481, 1682, 2105
MaeII	ACGT	3 cut(s)	30, 604, 2252
MaeIII	GTNAC	3 cut(s)	385, 1249, 1356
MbiI	CCGCTC	6 cut(s)	67, 393, 908, 924, 2414, 2450
MboII	GAAGA	9 cut(s)	16, 833, 1066, 1143, 1511, 1559, 2320, 2450, 2453
MfeI	CAATTG	2 cut(s)	527, 1536
MhlI	GDGCHC	6 cut(s)	86, 98, 131, 1156, 1328, 1545
MlsI	TGGCCA	1 cut(s)	1860
MluNI	TGGCCA	1 cut(s)	1860
MlyI	GAGTC	4 cut(s)	386, 648, 1040, 2147
MmeI	TCCRAC	4 cut(s)	165, 1474, 1801, 2057
Mox20I	TGGCCA	1 cut(s)	1860
Mph1103I	ATGCAT	2 cut(s)	1705, 2378
MroI	TCCGGA	2 cut(s)	550, 1743
MscI	TGGCCA	1 cut(s)	1860
MseI	TTAA	5 cut(s)	855, 1194, 1458, 1566, 2491
MslI	CAYNNNNRTG	3 cut(s)	593, 607, 2169
Msp20I	TGGCCA	1 cut(s)	1860
MspA1I	CMGCKG	3 cut(s)	304, 1448, 1616
MspI	CCGG	5 cut(s)	44, 551, 1744, 2035, 2168
MspR9I	CCNGG	7 cut(s)	534, 546, 1526, 2046, 2168, 2225, 2234
MunI	CAATTG	2 cut(s)	527, 1536
Mva1269I	GAATGC	2 cut(s)	1663, 1895
MvaI	CCWGG	6 cut(s)	534, 546, 1526, 2046, 2225, 2234
MvnI	CGCG	1 cut(s)	123
NciI	CCSGG	1 cut(s)	2168
NcoI	CCATGG	1 cut(s)	1941
NdeI	CATATG	1 cut(s)	981
NlaIV	GGNNCC	8 cut(s)	173, 524, 674, 730, 777, 1468, 1669, 1825
NmeAIII	GCCGAG	3 cut(s)	117, 945, 1927
NmuCI	GTSAC	1 cut(s)	385
NsbI	TGCGCA	1 cut(s)	1961
NsiI	ATGCAT	2 cut(s)	1705, 2378
NspI	RCATGY	6 cut(s)	612, 751, 883, 1480, 2242, 2380
OliI	CACNNNNGTG	1 cut(s)	593
PaeI	GCATGC	2 cut(s)	612, 883
PaeR7I	CTCGAG	2 cut(s)	783, 1488
PceI	AGGCCT	2 cut(s)	1138, 2232
PcsI	WCGNNNNNNNCGW	2 cut(s)	365, 386
PctI	GAATGC	2 cut(s)	1663, 1895
PfeI	GAWTC	4 cut(s)	554, 564, 1392, 1760
Pfl23II	CGTACG	2 cut(s)	1790, 2403
PleI	GAGTC	4 cut(s)	385, 647, 1039, 2146
PmaCI	CACGTG	1 cut(s)	605
PmlI	CACGTG	1 cut(s)	605
PpsI	GAGTC	4 cut(s)	385, 647, 1039, 2146
Ppu21I	YACGTR	2 cut(s)	31, 605
PpuMI	RGGWCCY	1 cut(s)	1668
PshAI	GACNNNNGTC	2 cut(s)	457, 828
Psp124BI	GAGCTC	1 cut(s)	98
Psp5II	RGGWCCY	1 cut(s)	1668
Psp6I	CCWGG	6 cut(s)	532, 544, 1524, 2044, 2223, 2232
PspCI	CACGTG	1 cut(s)	605
PspGI	CCWGG	6 cut(s)	532, 544, 1524, 2044, 2223, 2232
PspLI	CGTACG	2 cut(s)	1790, 2403
PspN4I	GGNNCC	8 cut(s)	173, 524, 674, 730, 777, 1468, 1669, 1825
PspPI	GGNCC	8 cut(s)	523, 548, 872, 1466, 1484, 1582, 1668, 1823
PspPPI	RGGWCCY	1 cut(s)	1668
PspXI	VCTCGAGB	1 cut(s)	1488
PstI	CTGCAG	4 cut(s)	451, 1144, 1333, 1615
PstNI	CAGNNNCTG	1 cut(s)	1451
PvuII	CAGCTG	3 cut(s)	304, 1448, 1616
RsaI	GTAC	6 cut(s)	29, 38, 74, 1005, 1792, 2405
RsaNI	GTAC	6 cut(s)	28, 37, 73, 1004, 1791, 2404
RseI	CAYNNNNRTG	3 cut(s)	593, 607, 2169
SacI	GAGCTC	1 cut(s)	98
SaqAI	TTAA	5 cut(s)	855, 1194, 1458, 1566, 2491
Sau96I	GGNCC	8 cut(s)	523, 548, 872, 1466, 1484, 1582, 1668, 1823
SbfI	CCTGCAGG	1 cut(s)	451
SchI	GAGTC	4 cut(s)	386, 648, 1040, 2147
ScrFI	CCNGG	7 cut(s)	534, 546, 1526, 2046, 2168, 2225, 2234
SdaI	CCTGCAGG	1 cut(s)	451
SduI	GDGCHC	6 cut(s)	86, 98, 131, 1156, 1328, 1545
SfcI	CTRYAG	5 cut(s)	447, 1140, 1329, 1611, 2092
Sfr274I	CTCGAG	2 cut(s)	783, 1488
SinI	GGWCC	6 cut(s)	523, 548, 872, 1466, 1668, 1823
SlaI	CTCGAG	2 cut(s)	783, 1488
SmiMI	CAYNNNNRTG	3 cut(s)	593, 607, 2169
SmlI	CTYRAG	4 cut(s)	783, 1095, 1488, 2243
SmoI	CTYRAG	4 cut(s)	783, 1095, 1488, 2243
SnaBI	TACGTA	1 cut(s)	31
SpeI	ACTAGT	1 cut(s)	1681
SphI	GCATGC	2 cut(s)	612, 883
Sse8387I	CCTGCAGG	1 cut(s)	451
SseBI	AGGCCT	2 cut(s)	1138, 2232
SspMI	CTAG	8 cut(s)	353, 626, 818, 876, 1226, 1481, 1682, 2105
SstI	GAGCTC	1 cut(s)	98
StuI	AGGCCT	2 cut(s)	1138, 2232
StyD4I	CCNGG	7 cut(s)	532, 544, 1524, 2044, 2166, 2223, 2232
StyI	CCWWGG	3 cut(s)	431, 995, 1941
TaaI	ACNGT	5 cut(s)	229, 584, 827, 1638, 1790
TaiI	ACGT	3 cut(s)	33, 607, 2255
TauI	GCSGC	2 cut(s)	108, 615
TfiI	GAWTC	4 cut(s)	554, 564, 1392, 1760
Tru1I	TTAA	5 cut(s)	855, 1194, 1458, 1566, 2491
Tru9I	TTAA	5 cut(s)	855, 1194, 1458, 1566, 2491
TscAI	CASTG	8 cut(s)	508, 595, 721, 894, 1333, 1983, 1998, 2459
TseFI	GTSAC	1 cut(s)	385
Tsp45I	GTSAC	1 cut(s)	385
TspDTI	ATGAA	8 cut(s)	17, 266, 554, 1200, 1543, 2451, 2455, 2461
TspGWI	ACGGA	2 cut(s)	1139, 1870
TspRI	CASTG	8 cut(s)	508, 595, 721, 894, 1333, 1983, 1998, 2459
VpaK11BI	GGWCC	6 cut(s)	523, 548, 872, 1466, 1668, 1823
XagI	CCTNNNNNAGG	1 cut(s)	945
XapI	RAATTY	2 cut(s)	895, 1914
XbaI	TCTAGA	3 cut(s)	625, 817, 1225
XceI	RCATGY	6 cut(s)	612, 751, 883, 1480, 2242, 2380
XhoI	CTCGAG	2 cut(s)	783, 1488
XspI	CTAG	8 cut(s)	353, 626, 818, 876, 1226, 1481, 1682, 2105
Zsp2I	ATGCAT	2 cut(s)	1705, 2378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.